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Gene information
General information
TC002081 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6092349..6097753
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC002081
      iBeetle screen iB_03297

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC002081 RA
        MTSVLLLCT LITPILANG FYHQEYNYQ TSSSSYKNH 
        ELQHHNEDE GFYSKDGDL EGRFRPRVN AHSQHSEYV 
        NPKMQHGYR IGTDRTSSF GQLHAQNRY RMNEPFEGG 
        AYGMVTPVH DYGISSNLR QVTANLQRE MEAELQRAV 
        YDHAQRAMA YGGITQAQI DTDRRVLEE ELRRNVTMR 
        LQEALKMQY GNQGIRGAY SYSIQNGRL QPTANYNNQ 
        ELADLTRQL EEELIRHLD QQVQTYYRT EVHTHTQRN 
        TYRVNGGEY PGALQARPP VYHQPYPPP HPNPVLAKP 
        DSITTVATR VQTELNEIL NKILEDTQK RYFAENSYQ 
        NPSINYDAI LAHLQDELR ANITFHFDD QIKRAYGYS 
        NYASKYSVE DIENLRRQI EANLITKLN RDFNRQRER 
        FFHTHQPMY QPIPANHVY YPVNQVHQV DVANLQRQM 
        QEQLSRQLQ EALMRQSYS QAMYSGHSG FNSQAHYQR 
        ALEQLSAEL NRNLTRQME QLNAQQYQI GVHDAQLAT 
        MRNQLQHDL MRQLQQGLQ QSYSQYSSW SSSSRSSSN 
        SHNYRPVRG FDANLYRQY GTSSDLVGE DCMMGDDPG 
        VKRTRRGAL TQENDDLTQ QTEDLTQQS QEIAQDEGQ 
        TEDLTQQTE DLTQQSQDM TQQTEDLTQ QSQDMTQQT 
        EDLTQQVQS EDSSGKPIR SKDVFWPLS QDLTQQSQD 
        LTQQSQDLT QQSQDLTQQ LQTQNDLEK PQRAEDVFE 
        SQSQDLTQQ TQDLTQPGK PKYQVQIGP AVSQEPTPH 
        YHAEYFRPS QDLTQQSQD LTQPKYHSQ IGPAVSQEP 
        TPHYHAEYY RPSQYLTQQ SQDLTQQTQ PGEPRYYSQ 
        LGPAVSQEP TPHYHAEYF RPSQDLTQQ SQDLTQPGK 
        PMYQSQMGP AVSHEPTPN YHAEYYRPS QYLTQQNQD 
        LTQQTQDLT QPGKPIYIS QNGPSVSQE PITRYYSKY 
        NPVAIDPVP QYQDYMRPS QDLTQPKLY SQTGPGVYQ 
        KRGPIVYHQ LDNVAPSQD LTQQSQDLT QPKSYSQIG 
        PTVYRKPSP IVYQEAEYD PTNQDLTQQ SQSMIGPPV 
        ESPTPKPIY AENIRLHSQ DLTQPIDQQ KPTDTNMKY 
        VVYDPTFYD SHQTPKRQY LTQQSQDLT QQTQDIQAS 
        KPYQSMLGS PVESYTPKP TYAEILRQH SQDLTQQSP 
        YQQKPNDTN MKYVMYNPI YYHSRQTPK NNDLTQQSQ 
        DLTLNNQGQ VDIGPPDSY QQSPVDTEP LQSQDLTQQ 
        SQDLTQQSQ DLTQQSQDL TQNSEPFNQ QQQNDYGSV 
        DPLYTSTYD WRPGKQNFG NSVGQRVNY FESLNKPTS 
        RPPKFDELR FQSENPSQD LTQQSQDLT QQSQDLTQQ 
        SQDLTQQTQ NLRKPIVGT PDLTQQSQD LTQQSQDLT 
        QQSQDLTQQ SQDLSQQSQ DLTQQDLTT PPTAQNSQK 
        KLSQAQEES QRLLDLLRR QSQENQNIG PMNYSPKPV 
        YADYGGQQI QVGTTRQLR PNEAPGRKL EPQTAQVDT 
        IPDQETQTV RDIEPVEPQ VASEVQQVD DTTGTTTKK 
        PGWWTRFKN KIG
      CDS sequence
      • >TC002081 RA
        atgacctcg gtgctcctc ctttgcacc ctcatcacc 
        ccaatcctc gccaacggc ttctaccac caagaatac 
        aactaccaa acatcctca tcgagttac aaaaaccac 
        gaactccag caccacaac gaagacgaa ggtttctat 
        tcgaaagat ggcgacttg gaggggcgg ttccgcccc 
        cgtgtcaac gcccacagc cagcacagc gaatacgtt 
        aacccgaaa atgcaacac ggttatcgc attggcacc 
        gatcgcacc tcctccttc ggccaactc cacgcccaa 
        aaccgctac cgaatgaac gaaccgttc gaaggcggg 
        gcttacgga atggtcact cctgtgcac gactacgga 
        atttcgtca aatttgcgc caagtaacg gcgaacttg 
        caacgcgaa atggaggcg gagctacag cgcgcggtt 
        tatgaccac gcccaacgc gcaatggcc tatggcggc 
        atcacgcaa gcgcagatt gacaccgat aggcgtgtg 
        ttagaggag gagcttcgg cgcaacgtc acaatgcga 
        ctacaggag gctttgaaa atgcaatac ggtaaccaa 
        gggatcaga ggggcgtat tcgtacagc atccaaaat 
        gggcgttta caaccaact gccaattac aacaaccag 
        gaattggcc gatttgacg cgacaattg gaggaggag 
        cttatccgc catcttgac caacaagtc cagacttac 
        taccggacg gaagtccac acccacaca caacgaaac 
        acctatcgg gtcaatggt ggggagtat ccaggggcg 
        ttacaggcc cgcccaccg gtgtaccac cagccctac 
        cccccacca caccctaac ccggttcta gccaaacct 
        gactcaatc acaaccgta gccacccga gtccaaacg 
        gaacttaac gaaatccta aataaaatt ttagaagat 
        actcaaaaa cgctacttt gccgaaaat tcctatcaa 
        aatccttcg attaattac gacgccatt ttggcacac 
        ctccaggac gaacttcgc gcaaacata accttccat 
        ttcgatgat caaatcaag cgtgcgtat ggttactca 
        aactatgcg tcaaaatac agcgttgag gacattgag 
        aatttgagg aggcaaatc gaggccaac ttgatcacc 
        aaacttaat cgtgatttt aaccgacag agagagcgc 
        tttttccac acccatcaa ccaatgtat caaccaatc 
        ccagccaat catgtctac taccccgtc aatcaagtg 
        catcaagtc gacgttgca aatttacaa agacaaatg 
        caagagcaa ctttcaagg caattacag gaggcgttg 
        atgcgtcaa tcgtactca caggcgatg tattctgga 
        cattctggt tttaattca caagctcat taccagagg 
        gcgctcgaa caactctca gctgagctt aatcgaaat 
        ttgacacga caaatggaa caattgaat gcgcaacag 
        taccagatt ggtgtacat gatgcacag cttgctacg 
        atgaggaat caattgcaa catgatttg atgaggcag 
        ttgcaacaa gggttgcaa cagtcctac agtcagtac 
        agctcgtgg agtagttcg tcgaggagt agttcaaat 
        agtcataat tataggcca gttagaggg tttgatgcc 
        aatttgtat aggcaatat ggcacctcc agtgatttg 
        gtgggggaa gattgtatg atgggggat gatccgggg 
        gttaaacgg acaaggagg ggagctttg acacaggaa 
        aatgacgat ttgacacaa cagaccgaa gatttgaca 
        cagcaaagt caagaaatt gcgcaggat gaaggacaa 
        accgaagat ctgacacaa cagactgaa gatttgaca 
        cagcaaagt caagatatg acacaacag acagaagat 
        ttgacacag caaagtcaa gatatgaca caacagact 
        gaagacttg acacagcaa gtccaaagt gaagacagt 
        tcgggcaaa ccgattaga tcaaaagac gttttttgg 
        cctttaagt caggatttg acacaacaa agtcaggat 
        ttgacacaa caaagtcag gatttgaca caacagagt 
        caagatttg acacagcaa cttcaaaca caaaacgat 
        ttagaaaaa ccgcaaaga gcagaagac gtttttgag 
        tctcaaagt caggatttg acacaacag actcaagat 
        ttaacacaa cctggcaaa cctaaatat caagtgcaa 
        ataggccca gctgtttcc caagaacct acacctcat 
        tatcacgct gaatatttt cgcccaagt caagatttg 
        acacagcaa agtcaagat ttgacacaa cctaagtat 
        cacagtcaa ataggtcca gctgtttct caagaacct 
        acacctcat tatcacgct gaatattat cgcccaagt 
        caatatttg acacaacaa agtcaggat ttgacacaa 
        caaactcaa cctggcgaa cctaggtat tatagtcaa 
        ttaggacca gctgtttcg caagaacct acacctcat 
        tatcacgct gaatatttt cgcccaagt caagatttg 
        acacagcaa agtcaagat ttgacacaa cctggcaaa 
        cctatgtat caaagtcaa atgggtcct gctgtttca 
        cacgaacct acacctaat tatcacgct gaatattat 
        cgcccaagt caatatttg acacaacaa aatcaggat 
        ttgacacaa caaactcaa gatttgaca caacctggc 
        aaacccatt tatataagt caaaacggt ccgagtgtt 
        tcacaagaa ccaataact cgttattat tccaagtat 
        aaccctgta gctatagat ccagtgcct caatatcaa 
        gactatatg cgtccaagt caagatttg acacaaccg 
        aagctttat agtcaaact ggtccaggt gtatatcaa 
        aaacgaggt ccaattgtc tatcaccaa cttgacaat 
        gtggcacca agtcaagat ttgacacag caaagtcaa 
        gatttgaca caacctaaa tcttatagt caaattggt 
        ccaactgtt tatcgtaaa ccatcgcca attgtgtat 
        caggaagct gaatatgac cccacaaac caagatttg 
        acacaacaa agtcaatcg atgattgga ccacctgtt 
        gaatccccc actccgaag cccatttat gccgaaaat 
        attagacta catagtcaa gatttgact caacctatc 
        gatcaacaa aagccaact gatacaaat atgaaatac 
        gtagtgtat gatcctaca ttttatgat tcacatcaa 
        acacccaaa cgtcaatat ttgacacaa caaagtcaa 
        gatttgaca caacaaacg caagatata caagcttcg 
        aaaccgtac caatcaatg cttggatca cctgttgaa 
        tcttacaca cctaaaccc acatatgcc gaaattctt 
        agacaacat agtcaagat ttgacacaa caatcgcct 
        tatcaacaa aaaccaaac gatacaaat atgaaatac 
        gtgatgtat aatcctata tattatcat tcacgtcaa 
        acacccaaa aataatgat ttgacacaa caaagtcaa 
        gatttgaca ctaaacaat caaggtcag gtagatatt 
        ggccctccg gactcttat cagcagtca ccggtggat 
        accgagcct ttacaaagt caagatttg acacaacaa 
        agtcaagat ttgacacaa caaagtcaa gatttgaca 
        caacagagt caagatttg acacaaaac agtgaacct 
        ttcaaccaa caacaacaa aatgactac ggatcagtc 
        gatcctcta tacacatca acatacgat tggcgccct 
        ggaaaacaa aactttggc aacagtgtt ggacaaagg 
        gtaaattat ttcgaaagt ctaaacaaa ccaacgtcg 
        cgaccgcca aaattcgat gagctgaga tttcagagt 
        gaaaatcca agtcaagat ttgacacag caaagtcaa 
        gatttgaca cagcaaagt caagatttg acacaacaa 
        agtcaagat ttgacacaa caaacgcaa aatcttaga 
        aaaccaatc gtaggcacg ccagacttg acacaacaa 
        agtcaagat ttgacacag caaagtcaa gatttgaca 
        cagcaaagt caagatttg acacaacaa agtcaagat 
        ttgtcacaa caaagtcaa gatttgaca caacaagac 
        ctcacaacg cctccaacg gcccaaaac tcccagaaa 
        aaattgtct caagcccaa gaggagtct caacgcctg 
        ctcgacctc ctccgaaga caatcacaa gaaaatcaa 
        aatattggc cccatgaac tactccccc aagccggtc 
        tatgccgac tatggtggc caacaaatc caggtcggt 
        accacccga caattgcgc cccaatgaa gcccccgga 
        agaaaactg gagccccaa actgcccaa gtggacaca 
        atcccggac caagaaaca caaactgtg cgagatatc 
        gaaccggtg gagccccaa gtggccagc gaagtgcaa 
        caagttgac gatactaca ggaacaaca acgaaaaag 
        cccggctgg tggacccga ttcaagaat aagataggt 
        taa
      mRNA sequence
      • >TC002081 RA
        atttttgtg aaagtgaaa gaatagtga agcaaaggg 
        cacaaaaat gacctcggt gctcctcct ttgcaccct 
        catcacccc aatcctcgc caacggctt ctaccacca 
        agaatacaa ctaccaaac atcctcatc gagttacaa 
        aaaccacga actccagca ccacaacga agacgaagg 
        tttctattc gaaagatgg cgacttgga ggggcggtt 
        ccgcccccg tgtcaacgc ccacagcca gcacagcga 
        atacgttaa cccgaaaat gcaacacgg ttatcgcat 
        tggcaccga tcgcacctc ctccttcgg ccaactcca 
        cgcccaaaa ccgctaccg aatgaacga accgttcga 
        aggcggggc ttacggaat ggtcactcc tgtgcacga 
        ctacggaat ttcgtcaaa tttgcgcca agtaacggc 
        gaacttgca acgcgaaat ggaggcgga gctacagcg 
        cgcggttta tgaccacgc ccaacgcgc aatggccta 
        tggcggcat cacgcaagc gcagattga caccgatag 
        gcgtgtgtt agaggagga gcttcggcg caacgtcac 
        aatgcgact acaggaggc tttgaaaat gcaatacgg 
        taaccaagg gatcagagg ggcgtattc gtacagcat 
        ccaaaatgg gcgtttaca accaactgc caattacaa 
        caaccagga attggccga tttgacgcg acaattgga 
        ggaggagct tatccgcca tcttgacca acaagtcca 
        gacttacta ccggacgga agtccacac ccacacaca 
        acgaaacac ctatcgggt caatggtgg ggagtatcc 
        aggggcgtt acaggcccg cccaccggt gtaccacca 
        gccctaccc cccaccaca ccctaaccc ggttctagc 
        caaacctga ctcaatcac aaccgtagc cacccgagt 
        ccaaacgga acttaacga aatcctaaa taaaatttt 
        agaagatac tcaaaaacg ctactttgc cgaaaattc 
        ctatcaaaa tccttcgat taattacga cgccatttt 
        ggcacacct ccaggacga acttcgcgc aaacataac 
        cttccattt cgatgatca aatcaagcg tgcgtatgg 
        ttactcaaa ctatgcgtc aaaatacag cgttgagga 
        cattgagaa tttgaggag gcaaatcga ggccaactt 
        gatcaccaa acttaatcg tgattttaa ccgacagag 
        agagcgctt tttccacac ccatcaacc aatgtatca 
        accaatccc agccaatca tgtctacta ccccgtcaa 
        tcaagtgca tcaagtcga cgttgcaaa tttacaaag 
        acaaatgca agagcaact ttcaaggca attacagga 
        ggcgttgat gcgtcaatc gtactcaca ggcgatgta 
        ttctggaca ttctggttt taattcaca agctcatta 
        ccagagggc gctcgaaca actctcagc tgagcttaa 
        tcgaaattt gacacgaca aatggaaca attgaatgc 
        gcaacagta ccagattgg tgtacatga tgcacagct 
        tgctacgat gaggaatca attgcaaca tgatttgat 
        gaggcagtt gcaacaagg gttgcaaca gtcctacag 
        tcagtacag ctcgtggag tagttcgtc gaggagtag 
        ttcaaatag tcataatta taggccagt tagagggtt 
        tgatgccaa tttgtatag gcaatatgg cacctccag 
        tgatttggt gggggaaga ttgtatgat gggggatga 
        tccgggggt taaacggac aaggagggg agctttgac 
        acaggaaaa tgacgattt gacacaaca gaccgaaga 
        tttgacaca gcaaagtca agaaattgc gcaggatga 
        aggacaaac cgaagatct gacacaaca gactgaaga 
        tttgacaca gcaaagtca agatatgac acaacagac 
        agaagattt gacacagca aagtcaaga tatgacaca 
        acagactga agacttgac acagcaagt ccaaagtga 
        agacagttc gggcaaacc gattagatc aaaagacgt 
        tttttggcc tttaagtca ggatttgac acaacaaag 
        tcaggattt gacacaaca aagtcagga tttgacaca 
        acagagtca agatttgac acagcaact tcaaacaca 
        aaacgattt agaaaaacc gcaaagagc agaagacgt 
        ttttgagtc tcaaagtca ggatttgac acaacagac 
        tcaagattt aacacaacc tggcaaacc taaatatca 
        agtgcaaat aggcccagc tgtttccca agaacctac 
        acctcatta tcacgctga atattttcg cccaagtca 
        agatttgac acagcaaag tcaagattt gacacaacc 
        taagtatca cagtcaaat aggtccagc tgtttctca 
        agaacctac acctcatta tcacgctga atattatcg 
        cccaagtca atatttgac acaacaaag tcaggattt 
        gacacaaca aactcaacc tggcgaacc taggtatta 
        tagtcaatt aggaccagc tgtttcgca agaacctac 
        acctcatta tcacgctga atattttcg cccaagtca 
        agatttgac acagcaaag tcaagattt gacacaacc 
        tggcaaacc tatgtatca aagtcaaat gggtcctgc 
        tgtttcaca cgaacctac acctaatta tcacgctga 
        atattatcg cccaagtca atatttgac acaacaaaa 
        tcaggattt gacacaaca aactcaaga tttgacaca 
        acctggcaa acccattta tataagtca aaacggtcc 
        gagtgtttc acaagaacc aataactcg ttattattc 
        caagtataa ccctgtagc tatagatcc agtgcctca 
        atatcaaga ctatatgcg tccaagtca agatttgac 
        acaaccgaa gctttatag tcaaactgg tccaggtgt 
        atatcaaaa acgaggtcc aattgtcta tcaccaact 
        tgacaatgt ggcaccaag tcaagattt gacacagca 
        aagtcaaga tttgacaca acctaaatc ttatagtca 
        aattggtcc aactgttta tcgtaaacc atcgccaat 
        tgtgtatca ggaagctga atatgaccc cacaaacca 
        agatttgac acaacaaag tcaatcgat gattggacc 
        acctgttga atcccccac tccgaagcc catttatgc 
        cgaaaatat tagactaca tagtcaaga tttgactca 
        acctatcga tcaacaaaa gccaactga tacaaatat 
        gaaatacgt agtgtatga tcctacatt ttatgattc 
        acatcaaac acccaaacg tcaatattt gacacaaca 
        aagtcaaga tttgacaca acaaacgca agatataca 
        agcttcgaa accgtacca atcaatgct tggatcacc 
        tgttgaatc ttacacacc taaacccac atatgccga 
        aattcttag acaacatag tcaagattt gacacaaca 
        atcgcctta tcaacaaaa accaaacga tacaaatat 
        gaaatacgt gatgtataa tcctatata ttatcattc 
        acgtcaaac acccaaaaa taatgattt gacacaaca 
        aagtcaaga tttgacact aaacaatca aggtcaggt 
        agatattgg ccctccgga ctcttatca gcagtcacc 
        ggtggatac cgagccttt acaaagtca agatttgac 
        acaacaaag tcaagattt gacacaaca aagtcaaga 
        tttgacaca acagagtca agatttgac acaaaacag 
        tgaaccttt caaccaaca acaacaaaa tgactacgg 
        atcagtcga tcctctata cacatcaac atacgattg 
        gcgccctgg aaaacaaaa ctttggcaa cagtgttgg 
        acaaagggt aaattattt cgaaagtct aaacaaacc 
        aacgtcgcg accgccaaa attcgatga gctgagatt 
        tcagagtga aaatccaag tcaagattt gacacagca 
        aagtcaaga tttgacaca gcaaagtca agatttgac 
        acaacaaag tcaagattt gacacaaca aacgcaaaa 
        tcttagaaa accaatcgt aggcacgcc agacttgac 
        acaacaaag tcaagattt gacacagca aagtcaaga 
        tttgacaca gcaaagtca agatttgac acaacaaag 
        tcaagattt gtcacaaca aagtcaaga tttgacaca 
        acaagacct cacaacgcc tccaacggc ccaaaactc 
        ccagaaaaa attgtctca agcccaaga ggagtctca 
        acgcctgct cgacctcct ccgaagaca atcacaaga 
        aaatcaaaa tattggccc catgaacta ctcccccaa 
        gccggtcta tgccgacta tggtggcca acaaatcca 
        ggtcggtac cacccgaca attgcgccc caatgaagc 
        ccccggaag aaaactgga gccccaaac tgcccaagt 
        ggacacaat cccggacca agaaacaca aactgtgcg 
        agatatcga accggtgga gccccaagt ggccagcga 
        agtgcaaca agttgacga tactacagg aacaacaac 
        gaaaaagcc cggctggtg gacccgatt caagaataa 
        gataggtta agacaagcg agttaattt attgtaatt 
        ttgatcgat tttaataaa atgtggtaa aactacacg 
        tttttgtgc acatacctg gggtaaatt gttgaaaaa 
        gtccacaaa taggcgttg ttgtttgaa atctgaaag 
        tgaggttac gtcacgtaa aaaatgtta cgtaaaggt 
        ggtaaagaa aaataccta aatttagcg cttccgcgg 
        ggtgtgtta ttgacattg ttgcggttt ttggcacaa 
        ttaacacac aagttacgc tattaattg tttaattaa 
        ttgttagtg gcgggaatg ttgaactgt cactggtga 
        gtcagagtg ggcaacaca aaaccacca caatcacca 
        gttttgttt gtttttact acgt
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