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Gene information
General information
TC002080 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6101259..6105343
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002080
    iBeetle screen iB_03296

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    larva appendages sometimes shape irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages sometimes position irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    legs podomer partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    posterior terminus sometimes lighter - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002080 RA
      MDPMLQRHC ARYIYVVPD GLRELLSDI SREVLRSEP 
      EDIYTFIAD YLDALLITR ENARISAKF VQYMTEVAE 
      TIVDLLKRT GMSREEADA AANVIQEAF KSWSERKGI 
      LKEDEDLMI ARIIEEAGI TEGQVHAAA VVIQHAFRA 
      FKARQQREA QLLSGIVDW RVAARSAIR LYRKSGVSP 
      EEAKRAANL IKAAYKGYY TRRAIRQLN EEPVQEEER 
      EEEVEEEES LGSFYSEHR KSKGVRIDF NTVVPHVDF 
      GGEELISRD IVSVLEEEQ PPHDDNIMA RIFGELPEP 
      TVDEIVNDI LNDIIIGAI GTEEEPHVI LEEMDSDID 
      SFVPPLVEE KQEATMNRS QILPVMSQE ELDMEMREA 
      RLEMLALKR ASRERQSAI EIEQVERVV TPVIEEEEN 
      E
    CDS sequence
    • >TC002080 RA
      atggatcca atgctgcaa cgccactgc gcccgctac 
      atctacgtc gtccccgac ggcctccgg gagctcctg 
      agcgacatc tcccgagaa gtcctacgc tccgaaccc 
      gaggacatc tacaccttc atcgccgac tacctggac 
      gccctcctc ataacgcgc gaaaacgcc cgaatttcc 
      gccaaattc gtccaatac atgaccgaa gtggcggaa 
      accatcgtc gacctcctc aaacgcacc ggaatgagc 
      cgagaggag gccgacgcc gccgccaac gtcatccag 
      gaagctttc aaatcgtgg agcgaacgg aagggaatc 
      ctgaaagaa gacgaagat ttgatgatt gcgcgcatt 
      attgaagaa gcgggaatc accgaaggg caagtccat 
      gccgccgcc gttgtgatc caacacgcg ttccgagct 
      ttcaaagca cggcaacag agggaggca cagttgttg 
      agcgggatt gtggattgg agagtggct gcgcgaagt 
      gccattcgg ttgtatcgc aaatccggg gtttcaccc 
      gaagaggcc aaaagggcc gccaacttg atcaaggcc 
      gcctataaa ggctactac acgcgaagg gccatcaga 
      caactgaac gaagaacca gtgcaagag gaagaaagg 
      gaggaggag gtggaagaa gaggagtcg ctagggtcg 
      ttctatagc gaacacagg aagtcaaag ggggtccga 
      attgacttc aatacggtg gtaccgcac gtcgatttc 
      ggtggtgag gagctaatt tcgcgcgac atagtctca 
      gttttggag gaggagcaa cccccacac gacgataac 
      atcatggcg aggattttc ggggaactc cccgaaccc 
      acagttgac gaaatcgtc aatgacatc ctcaatgac 
      ataataatt ggggccata gggactgaa gaagagcct 
      cacgttatc ttggaagag atggattct gacatagac 
      tcgtttgtt cctcctctg gttgaagaa aaacaagaa 
      gctactatg aatcgttcg cagattctc ccggtgatg 
      tctcaggag gagttggat atggagatg agggaagcc 
      aggctggag atgttggct ttgaagagg gcgtcaagg 
      gagagacag tcggcgatt gagatcgaa caggtggaa 
      agggttgtt acaccagtg attgaggag gaggaaaat 
      gagtaa
    mRNA sequence
    • >TC002080 RA
      catgaaaga caatgggac ttgtaattt cggaaaaag 
      aagtcaaaa atccagttt tccaaatgc cagtttcga 
      caatttcgc tcccttcat cagtcaaat tttgacacc 
      cgtgtcctg ccatggatc caatgctgc aacgccact 
      gcgcccgct acatctacg tcgtccccg acggcctcc 
      gggagctcc tgagcgaca tctcccgag aagtcctac 
      gctccgaac ccgaggaca tctacacct tcatcgccg 
      actacctgg acgccctcc tcataacgc gcgaaaacg 
      cccgaattt ccgccaaat tcgtccaat acatgaccg 
      aagtggcgg aaaccatcg tcgacctcc tcaaacgca 
      ccggaatga gccgagagg aggccgacg ccgccgcca 
      acgtcatcc aggaagctt tcaaatcgt ggagcgaac 
      ggaagggaa tcctgaaag aagacgaag atttgatga 
      ttgcgcgca ttattgaag aagcgggaa tcaccgaag 
      ggcaagtcc atgccgccg ccgttgtga tccaacacg 
      cgttccgag ctttcaaag cacggcaac agagggagg 
      cacagttgt tgagcggga ttgtggatt ggagagtgg 
      ctgcgcgaa gtgccattc ggttgtatc gcaaatccg 
      gggtttcac ccgaagagg ccaaaaggg ccgccaact 
      tgatcaagg ccgcctata aaggctact acacgcgaa 
      gggccatca gacaactga acgaagaac cagtgcaag 
      aggaagaaa gggaggagg aggtggaag aagaggagt 
      cgctagggt cgttctata gcgaacaca ggaagtcaa 
      agggggtcc gaattgact tcaatacgg tggtaccgc 
      acgtcgatt tcggtggtg aggagctaa tttcgcgcg 
      acatagtct cagttttgg aggaggagc aacccccac 
      acgacgata acatcatgg cgaggattt tcggggaac 
      tccccgaac ccacagttg acgaaatcg tcaatgaca 
      tcctcaatg acataataa ttggggcca tagggactg 
      aagaagagc ctcacgtta tcttggaag agatggatt 
      ctgacatag actcgtttg ttcctcctc tggttgaag 
      aaaaacaag aagctacta tgaatcgtt cgcagattc 
      tcccggtga tgtctcagg aggagttgg atatggaga 
      tgagggaag ccaggctgg agatgttgg ctttgaaga 
      gggcgtcaa gggagagac agtcggcga ttgagatcg 
      aacaggtgg aaagggttg ttacaccag tgattgagg 
      aggaggaaa atgagtaaa aatgacaca aatacgaaa 
      g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY