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Gene information
General information
TC002077 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid75: 1856887..1858895
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002077
    iBeetle screen iB_03295
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002077 RA
      MAKNNLNGD SDVSSNCED DEMVLYKKL SLNVITFRT 
      VRDKQDLED PPRKGSSFK PDMAHQIFG ETEMIFGYK 
      KLAINLFYL HNSAKCYVE VQTGGKIHE NADDIMKAL 
      NPWLPENYR TEKSDFLAD LDEEKPGLM FGDVIDTFS 
      TDSENVYKV TVCDLKNPD FRQFHQRFE TLIVWFIDG 
      ANFIDLEDP RWVIFYVYE EGPESHFTP VGFCTVYKF 
      FLYPNLTRP RISQFFVLP THQKRGIGT NLYKAVYNY 
      LKQMPDSGD ITVEEPTEV FQKIRDFCD SLHIYEDLE 
      GKGINITTQ NLKQINEFL KKHKIGKRQ AQRVFDILE 
      CLTAHKNGY KEYVKFTEG IRSRIANEI EKETRGSKR 
      LCNLERAGI VTEPIDKKA AIDAEYNKY VDSLEWPVN 
      RLRALLVKK
    CDS sequence
    • >TC002077 RA
      atggctaaa aacaacctt aacggagac agtgatgtt 
      tcctcgaac tgcgaagac gacgaaatg gtcttgtac 
      aagaagctg agcctcaac gtgatcacg ttccggacc 
      gtgcgagac aagcaggat ctggaggac cccccgagg 
      aagggcagc tcgtttaag cccgacatg gcccaccag 
      atattcggg gagacagag atgattttt gggtacaaa 
      aaactggcc ataaatttg ttttatttg cacaattcg 
      gccaagtgt tacgttgag gtgcaaacc ggggggaaa 
      atacacgaa aacgccgat gatatcatg aaagcgcta 
      aatccctgg ctgccggaa aactaccgg actgaaaag 
      agcgatttt ctcgccgat ttggacgag gaaaagccc 
      ggtctgatg ttcggggac gtgatcgac acgttttcc 
      accgattct gaaaatgtg tataaagtg acagtttgc 
      gacttgaaa aaccccgac ttccgccag ttccaccaa 
      cgtttcgag acgctaatc gtgtggttc atcgacggc 
      gccaacttc atagacctg gaggacccc cgctgggtg 
      atcttctac gtctatgaa gagggcccc gagtcgcac 
      ttcacccca gtcggtttt tgcaccgtt tacaaattt 
      ttcctctac cccaactta acgcgcccc cgaattagc 
      caatttttc gtgctcccc acgcaccag aagcgcgga 
      atcggcacg aatttgtac aaagctgtt tacaattac 
      ttgaaacaa atgcccgac tcgggcgat attacagtg 
      gaagaaccg accgaggtg ttccagaaa atacgcgat 
      ttttgcgac agcttgcac atttatgag gatttggag 
      gggaaaggg atcaacatc acgacgcaa aatctgaag 
      caaattaac gagtttttg aagaaacac aagattggg 
      aaacggcaa gcgcagcgc gtttttgat attttagaa 
      tgtctgact gcgcataaa aacgggtat aaggagtac 
      gtcaagttc acggagggg attcggagc aggattgcc 
      aacgagatt gagaaggag actcggggc agtaagaga 
      ttgtgtaac ttggagcgg gccggaatt gtgaccgaa 
      cctatcgac aaaaaggca gctattgat gcagagtat 
      aataaatat gtggatagt ttggagtgg cctgtgaat 
      cggttgagg gcactgttg gtcaagaag tga
    mRNA sequence
    • >TC002077 RA
      caaatttcc cgccacatt acgtgacat gcgcaaaaa 
      atcgcgcgc cagccgact tgaccacag ttaactagt 
      gttcggtta tgtggcccc aaaggccct caaaattgt 
      tctaagtgt tttcaattt cgaaatttg tggtcacgt 
      atcacttac gccgtcttt tatcacggc acagtcact 
      ttttgtcgg aaaaaaaac ggcaaaaaa ttcgagatg 
      gctaaaaac aaccttaac ggagacagt gatgtttcc 
      tcgaactgc gaagacgac gaaatggtc ttgtacaag 
      aagctgagc ctcaacgtg atcacgttc cggaccgtg 
      cgagacaag caggatctg gaggacccc ccgaggaag 
      ggcagctcg tttaagccc gacatggcc caccagata 
      ttcggggag acagagatg atttttggg tacaaaaaa 
      ctggccata aatttgttt tatttgcac aattcggcc 
      aagtgttac gttgaggtg caaaccggg gggaaaata 
      cacgaaaac gccgatgat atcatgaaa gcgctaaat 
      ccctggctg ccggaaaac taccggact gaaaagagc 
      gattttctc gccgatttg gacgaggaa aagcccggt 
      ctgatgttc ggggacgtg atcgacacg ttttccacc 
      gattctgaa aatgtgtat aaagtgaca gtttgcgac 
      ttgaaaaac cccgacttc cgccagttc caccaacgt 
      ttcgagacg ctaatcgtg tggttcatc gacggcgcc 
      aacttcata gacctggag gacccccgc tgggtgatc 
      ttctacgtc tatgaagag ggccccgag tcgcacttc 
      accccagtc ggtttttgc accgtttac aaatttttc 
      ctctacccc aacttaacg cgcccccga attagccaa 
      tttttcgtg ctccccacg caccagaag cgcggaatc 
      ggcacgaat ttgtacaaa gctgtttac aattacttg 
      aaacaaatg cccgactcg ggcgatatt acagtggaa 
      gaaccgacc gaggtgttc cagaaaata cgcgatttt 
      tgcgacagc ttgcacatt tatgaggat ttggagggg 
      aaagggatc aacatcacg acgcaaaat ctgaagcaa 
      attaacgag tttttgaag aaacacaag attgggaaa 
      cggcaagcg cagcgcgtt tttgatatt ttagaatgt 
      ctgactgcg cataaaaac gggtataag gagtacgtc 
      aagttcacg gaggggatt cggagcagg attgccaac 
      gagattgag aaggagact cggggcagt aagagattg 
      tgtaacttg gagcgggcc ggaattgtg accgaacct 
      atcgacaaa aaggcagct attgatgca gagtataat 
      aaatatgtg gatagtttg gagtggcct gtgaatcgg 
      ttgagggca ctgttggtc aagaagtga cctgtttat 
      ttattctca tttttgcat ttttcatta aattttcac 
      taataaagg tttttctac tag