Status
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Gene information
General information
TC002074 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6129018..6142082
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002074
    iBeetle screen iB_03294

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content darker - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection


    Fertility
    egg chamber number decreased - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late potentiallyfused - Number of animals showing the phenotype: 2

    vitellogenic egg chamber late degenerated - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002074 RA
      MEAKPSKMS RLFQTSSVE SKDEYEEKL ERSYQAVTA 
      LTAGLSDKD AYDAITKAL LKDKGYEEV SLGLLVTIL 
      TEPHNAARG YRDLTLISN DGFGLVLNS LSQLVLDRY 
      LRFTDVVKG QVVWLLREM IKNAVNNVD NLCWNLMRH 
      AAGGDVSAK NIALIESLL DVLVEYRSW LDKFPLLIP 
      SVVYTYLRL VEDHNAPYL ANLRQKEVN FLISLIRER 
      FNDCIGIGR DLVRLLQNV ARIPEFEQL WKDILTAPR 
      TLSPTFTGV LQLLQARTS RRYLQSRLT PDMERKLVF 
      FTSQVRFGN HKRYQDWFQ RQYLATPES QSLRCDIIR 
      FIVGVIHPT NELLCSDII PRWAVIGWL LTTCTSSVA 
      VSNAKLALF YDWLFFDSE KDNIMNIEP AILVMHHSM 
      RSHPAVSAT LLDFLCRII PNFHQPLTE KVRNGIFSS 
      LRKILEKRV VPSLVPLFD NGKLDPELR NMVRETFKE 
      FCAATVPES SGVNNHVNN HVLDNEPAF SDDEEEQPL 
      TICALDDDS DEDDDDIPL SKVRLRKVQ DKLDESIDG 
      PLGEILTKL QLEKDRESR CGHMETFLQ TLMGFEGDD 
      DDNLPMIAK TLANIIQDD HKCGELKPE QMTDDELLE 
      SIKLPLFVL FQTLYNFTK QDEDGKEIL NRLLAEMRN 
      CFPTVGYFL LYFLKVQIR TENKREDQT KASALKVSV 
      YKEYCQSCD KKIDVCLLD DLIACHISD TKLMMWIVP 
      DLFRDFKQQ TVNNAQIIR VIISAIDSR QLQTLVGKV 
      LQGHLVMFK SENLVPLLK ITLTWESIE QFFFWQLIH 
      AHDISIDTV LPLVTQLDY EQHSEALTA ITLMLKQER 
      PNADYVKYL FSRDLSPSG DLFIFTIVK YWCDEYIDK 
      VAELISSLL STRYPSTSP NKRKRSGNK TLSATVPSA 
      DQVLGHLEQ LRVICERHD CMAIYTLDS MQRALNIAM 
      NNCSDGQKK QYGQLFALL EEEEEVSSA TKSRSQGRG 
      RKPVNKSAV ATKRTAVRE ICDSSEESS EEEEIVKPK 
      QPKKRKKVM SDSD
    CDS sequence
    • >TC002074 RA
      atggaagcg aagccttcg aagatgtcc cgtctgttc 
      cagacgtcg tccgtcgaa tccaaagac gaatacgag 
      gagaaactc gaacgcagc taccaggcc gtcaccgcc 
      ctgacggcg ggcctcagc gacaaggac gcctacgac 
      gccatcacc aaggccctg ctcaaggac aagggctac 
      gaggaggtg tcgctgggc ctgctggtc accatcctc 
      accgagccg cacaacgcc gcccgcggc taccgcgac 
      ctcaccctc atctccaac gacggcttc ggtctggtg 
      ctcaactcg ctctcgcag ctggtgctc gaccgctac 
      ctgcgcttc accgacgtg gtcaagggc caggtggtg 
      tggctgctc cgggagatg atcaagaac gcggtcaac 
      aacgtcgac aacctctgc tggaacctg atgcggcac 
      gcggccggc ggcgacgtc tccgccaag aacatcgcg 
      ctcatcgag agcctcctc gacgtgctg gtggagtat 
      cgcagttgg ctggacaag ttcccgctg ctcataccc 
      tcggttgtt tacacctac ttgcgattg gtggaggac 
      cacaatgcg ccttattta gccaactta agacagaag 
      gaagttaat tttttaatt agtttgatt agggaacgg 
      ttcaatgac tgtatcggg atcgggcgt gacctagtg 
      agactcttg cagaacgtt gccagaatc ccggaattt 
      gaacaactc tggaaggat attctcact gcccccagg 
      acgctcagt cccacgttt acgggggtt ttgcaactg 
      ctgcaagcg agaacgtcc aggaggtac ctccagtcg 
      aggctcacg ccggacatg gagagaaaa ctcgtcttt 
      tttacgtcc caggtccgt ttcggcaac cacaaacgt 
      taccaggac tggttccaa cgccaatac ctcgccacc 
      cccgaatcg cagagcctc cgttgcgac ataatccgt 
      ttcatcgtt ggcgtaatc catccaaca aacgaactt 
      ctctgctcg gatataatc ccccgttgg gcggtcatc 
      ggttggctg ttaaccaca tgcacctca tccgtagcc 
      gtctcgaac gccaaactg gccctcttc tacgattgg 
      ctgttcttc gacagcgaa aaagacaac attatgaac 
      attgaaccg gcgatctta gtaatgcac cattcgatg 
      cgttcacat cctgccgtc agtgcaacc ctacttgat 
      tttttatgc cgaattatc cccaacttc caccaaccg 
      ttaaccgaa aaagtacga aacggtatt ttctcatcg 
      ctgcgtaaa atactagag aaacgggtg gtgccgtcg 
      ttggtgcca ctcttcgac aatgggaaa cttgacccg 
      gagctgagg aacatggta cgggagacg tttaaggag 
      ttttgtgct gcgacggtc ccggaaagc tccggggtc 
      aacaaccac gtgaataat catgttttg gacaacgaa 
      ccggcgttt agtgacgat gaggaggag cagccgttg 
      acgatttgt gcccttgac gacgacagc gacgaggat 
      gatgacgat ataccgttg tcgaaggtg cggttgcgg 
      aaggtccag gataagttg gacgagagt atagatggg 
      cctttgggg gagattttg accaagtta cagctggag 
      aaggatcgg gagagtcgc tgtggtcac atggagact 
      tttcttcaa accttgatg gggttcgag ggtgacgat 
      gacgataat ctaccaatg atcgcaaag actttggcc 
      aacataatc caagacgat cacaagtgc ggtgagctc 
      aagccggaa caaatgact gacgatgag ctgcttgaa 
      agcatcaag ttgccactg tttgtcctc ttccaaacg 
      ctgtacaat ttcacgaaa caggacgag gacggcaaa 
      gagatcctc aatcgctta ttggccgag atgcgcaat 
      tgtttcccc accgtcggc tacttcctc ctctatttc 
      ctcaaagtt caaatacgg acagagaac aaacgcgag 
      gaccagacc aaagcaagt gcgctgaaa gtgagtgtg 
      tacaaggag tattgccaa tcgtgtgat aaaaaaata 
      gacgtttgt ttgctcgac gacttgatc gcatgccac 
      atcagtgat accaaacta atgatgtgg atagtgcct 
      gatttattt agggatttt aaacaacaa actgttaac 
      aacgctcaa ataatacgt gtgataatc tcagcgatt 
      gactctcgc caattacaa acgctagtc ggtaaagtg 
      cttcaaggt catttagtg atgttcaaa agtgagaat 
      ctggtgccg ttgcttaaa atcacgcta acgtgggaa 
      agtatcgag cagtttttt ttctggcaa ctgatacac 
      gcccacgat atttccatt gacactgtt ctaccattg 
      gtaacacaa ctggactat gagcagcat tcggaagcg 
      ctaactgcg atcacgctg atgctgaag caggagcgg 
      cccaacgcc gactatgtc aagtattta ttttcgcgt 
      gatttgtcg ccttccggc gatttgttt atttttacc 
      attgttaag tactggtgt gatgagtat attgataag 
      gtggctgag ttgatttcg tcgttgttg agtacgcgg 
      tatccgtca acgtcgccg aataagcgg aaacgtagc 
      gggaataag acgctgagt gcgacggtg ccgtcggcc 
      gaccaggtc ctggggcat ttggaacag ttgagggtg 
      atttgtgag cggcatgac tgtatggcg atctatacg 
      ctggattcg atgcagagg gcgctgaat atcgcgatg 
      aataattgt agtgatggg cagaagaag cagtacgga 
      cagttgttt gccttgttg gaggaggag gaggaggtt 
      agtagtgcg acgaagagt cgctcgcaa gggaggggg 
      aggaagccg gtgaataag agtgcggtg gcgacgaag 
      aggacggcg gttagggag atttgtgat tcgtcggag 
      gagagtagc gaggaggag gagatcgtg aagccaaag 
      cagcctaag aagcgtaaa aaagtgatg tccgacagt 
      gattga
    mRNA sequence
    • >TC002074 RA
      tgctacact acctcaaaa attaaaaat cagcgttga 
      cttttttct ctattcatt tgtaccaaa ttttcaaat 
      ttcgcgcca tgacgacac acaatcctt gcggcaaat 
      ggaggggag agctggtta agtcattgc cgatttttg 
      gggttattt tgaaatttg gcgccacaa agatgctaa 
      caagaggtt ggtgaacct ggctttacg atgggtttt 
      ttaatcaac aacaaactg ataaatttg ggcaaatat 
      tgacacata cgaacccgt cacgtgtct tcaccgccg 
      gcggtccaa aaatcacaa gtccgtccc tgtttccgg 
      tgataaacc gcgattttg cacattttt ccgtcactt 
      cactcacta aaacctact tcactcccg aaatcaaaa 
      tttaaatcg tgtaaaaaa cgtcgtttt cggtcgttt 
      atgatcgtg cgtttccgt gtcgaaaca cgacgtata 
      tatcaccgc gcgcttgat ttccacaaa cattttccg 
      aggatggga cgatgcctt gccttccgt gtttgtcaa 
      tcgttgtcg tcttctagt gtccgttaa gcgtatcgg 
      ggccgatgg aagcgaagc cttcgaaga tgtcccgtc 
      tgttccaga cgtcgtccg tcgaatcca aagacgaat 
      acgaggaga aactcgaac gcagctacc aggccgtca 
      ccgccctga cggcgggcc tcagcgaca aggacgcct 
      acgacgcca tcaccaagg ccctgctca aggacaagg 
      gctacgagg aggtgtcgc tgggcctgc tggtcacca 
      tcctcaccg agccgcaca acgccgccc gcggctacc 
      gcgacctca ccctcatct ccaacgacg gcttcggtc 
      tggtgctca actcgctct cgcagctgg tgctcgacc 
      gctacctgc gcttcaccg acgtggtca agggccagg 
      tggtgtggc tgctccggg agatgatca agaacgcgg 
      tcaacaacg tcgacaacc tctgctgga acctgatgc 
      ggcacgcgg ccggcggcg acgtctccg ccaagaaca 
      tcgcgctca tcgagagcc tcctcgacg tgctggtgg 
      agtatcgca gttggctgg acaagttcc cgctgctca 
      taccctcgg ttgtttaca cctacttgc gattggtgg 
      aggaccaca atgcgcctt atttagcca acttaagac 
      agaaggaag ttaattttt taattagtt tgattaggg 
      aacggttca atgactgta tcgggatcg ggcgtgacc 
      tagtgagac tcttgcaga acgttgcca gaatcccgg 
      aatttgaac aactctgga aggatattc tcactgccc 
      ccaggacgc tcagtccca cgtttacgg gggttttgc 
      aactgctgc aagcgagaa cgtccagga ggtacctcc 
      agtcgaggc tcacgccgg acatggaga gaaaactcg 
      tctttttta cgtcccagg tccgtttcg gcaaccaca 
      aacgttacc aggactggt tccaacgcc aatacctcg 
      ccacccccg aatcgcaga gcctccgtt gcgacataa 
      tccgtttca tcgttggcg taatccatc caacaaacg 
      aacttctct gctcggata taatccccc gttgggcgg 
      tcatcggtt ggctgttaa ccacatgca cctcatccg 
      tagccgtct cgaacgcca aactggccc tcttctacg 
      attggctgt tcttcgaca gcgaaaaag acaacatta 
      tgaacattg aaccggcga tcttagtaa tgcaccatt 
      cgatgcgtt cacatcctg ccgtcagtg caaccctac 
      ttgattttt tatgccgaa ttatcccca acttccacc 
      aaccgttaa ccgaaaaag tacgaaacg gtattttct 
      catcgctgc gtaaaatac tagagaaac gggtggtgc 
      cgtcgttgg tgccactct tcgacaatg ggaaacttg 
      acccggagc tgaggaaca tggtacggg agacgttta 
      aggagtttt gtgctgcga cggtcccgg aaagctccg 
      gggtcaaca accacgtga ataatcatg ttttggaca 
      acgaaccgg cgtttagtg acgatgagg aggagcagc 
      cgttgacga tttgtgccc ttgacgacg acagcgacg 
      aggatgatg acgatatac cgttgtcga aggtgcggt 
      tgcggaagg tccaggata agttggacg agagtatag 
      atgggcctt tgggggaga ttttgacca agttacagc 
      tggagaagg atcgggaga gtcgctgtg gtcacatgg 
      agacttttc ttcaaacct tgatggggt tcgagggtg 
      acgatgacg ataatctac caatgatcg caaagactt 
      tggccaaca taatccaag acgatcaca agtgcggtg 
      agctcaagc cggaacaaa tgactgacg atgagctgc 
      ttgaaagca tcaagttgc cactgtttg tcctcttcc 
      aaacgctgt acaatttca cgaaacagg acgaggacg 
      gcaaagaga tcctcaatc gcttattgg ccgagatgc 
      gcaattgtt tccccaccg tcggctact tcctcctct 
      atttcctca aagttcaaa tacggacag agaacaaac 
      gcgaggacc agaccaaag caagtgcgc tgaaagtga 
      gtgtgtaca aggagtatt gccaatcgt gtgataaaa 
      aaatagacg tttgtttgc tcgacgact tgatcgcat 
      gccacatca gtgatacca aactaatga tgtggatag 
      tgcctgatt tatttaggg attttaaac aacaaactg 
      ttaacaacg ctcaaataa tacgtgtga taatctcag 
      cgattgact ctcgccaat tacaaacgc tagtcggta 
      aagtgcttc aaggtcatt tagtgatgt tcaaaagtg 
      agaatctgg tgccgttgc ttaaaatca cgctaacgt 
      gggaaagta tcgagcagt tttttttct ggcaactga 
      tacacgccc acgatattt ccattgaca ctgttctac 
      cattggtaa cacaactgg actatgagc agcattcgg 
      aagcgctaa ctgcgatca cgctgatgc tgaagcagg 
      agcggccca acgccgact atgtcaagt atttatttt 
      cgcgtgatt tgtcgcctt ccggcgatt tgtttattt 
      ttaccattg ttaagtact ggtgtgatg agtatattg 
      ataaggtgg ctgagttga tttcgtcgt tgttgagta 
      cgcggtatc cgtcaacgt cgccgaata agcggaaac 
      gtagcggga ataagacgc tgagtgcga cggtgccgt 
      cggccgacc aggtcctgg ggcatttgg aacagttga 
      gggtgattt gtgagcggc atgactgta tggcgatct 
      atacgctgg attcgatgc agagggcgc tgaatatcg 
      cgatgaata attgtagtg atgggcaga agaagcagt 
      acggacagt tgtttgcct tgttggagg aggaggagg 
      aggttagta gtgcgacga agagtcgct cgcaaggga 
      gggggagga agccggtga ataagagtg cggtggcga 
      cgaagagga cggcggtta gggagattt gtgattcgt 
      cggaggaga gtagcgagg aggaggaga tcgtgaagc 
      caaagcagc ctaagaagc gtaaaaaag tgatgtccg 
      acagtgatt gacggaacc aagtcgtag cagtggatt 
      cgtcgtgtg tgaagcaca tcggaatta aggaacgtc 
      atatcagat gcaagcttg tgatcatta gcattattc 
      agagacacg tactatggt gaagaggaa aggaaataa 
      taataaaaa atgagcgga ggaataact aaatgaagt 
      gaatacact gcctgtaga caagattca aagacaaat 
      atcgatgtc ttgattgaa taaacacaa ttgttgagt 
      accttaaga aacgttgtt aattattgt attatttta 
      catgtttat cagtaactc tcatgtgta ttctgtatt 
      atattttct gtaaattgt tactgttag gttgtacca 
      tcctctgtt gtcattttt gctattcaa gggtctatt 
      attagaaca agtttttaa gcgaactgt aaattgata 
      gtgttttat tacaagcgg atgtataaa tttgtaaat 
      atatttata aagagcagg atatgtttt gacacggag 
      tagtgtccc cgtacgatc aatataatt tgttttatt 
      ac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY