Status
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Gene information
General information
TC002072 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007425.3: 6107775..6127524
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002072
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03293

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 4 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002072 RA
      MATLCPGVQ YGLSSQQNF GENKDLIFV KLTDSAVRA 
      IYDFLKNQN KFNCQGPTI KFLGNEGQL SFPSVQNGY 
      TSFSFSMSS TADMEGPGG SFECVQQTG PPRGSLEVL 
      GSIPYKVRV RANDDIYEA TRHRMTIAE ENHKNKCTR 
      EIKPNEKDI GRKVKLKQS RASLPPIRR EPPPPAPKP 
      SYQPSKPAP PPPVISSSN GLSNGLGSN HVSSSRPQM 
      NKPSVPDIA RRPIKERLI HLLALRPFK KVELHDRIT 
      KEGVREKNG ITSILKQIS FMKDNCYHL NRAMWNEVN 
      EDWPFYTES ERQMLKRRK PANLTPPGS SDGGSSGSG 
      QSPTSTHPG SPPPISTTS SSSKRPGYY DNQDGLPTK 
      RQRISHYRK PSEQNYRSP IENNSSQRR PPTDSRDAS 
      NMNPRSRES PTTATATAA AAAAPTVAL SNGDTKLRA 
      DDDKQRCDS HSLSYGGRN SPLTNNKTT TTENGYVNG 
      KCTPREKER MRERVSSSS QRSVRVSPD SQSEAMGGG 
      DRVSPIREK GAVAKVDSS SEFPDYLTE YTTIKDADQ 
      RRRFKADFN ADYAEYKDL HGIVEKVSR RFVDLELKL 
      KQEDASSPR FKDLKKQIV REYNENKND LEHQKLKRR 
      FQYLHDKLS HIKRLVLEY DQGLANNRY
    CDS sequence
    • >TC002072 RA
      atggcgact ctctgtcca ggtgttcag tatggttta 
      tcatcgcag caaaatttc ggtgaaaat aaagactta 
      atttttgtg aaactgacg gattccgcc gttcgtgca 
      atatacgac tttttaaaa aatcagaat aaattcaac 
      tgccagggt cccacgata aagttccta ggaaatgaa 
      gggcaatta tctttcccc tccgtgcaa aacggctac 
      acttcgttc agtttttcg atgtctagt acggcggac 
      atggagggc cccgggggc agtttcgaa tgcgtccag 
      cagacgggt cccccccgg ggctccctc gaagtcctc 
      ggttcgata ccatacaaa gtccgtgtc cgcgccaat 
      gacgacatc tacgaagcc acccgtcat cgtatgacc 
      atcgccgaa gaaaatcac aagaacaaa tgcacaagg 
      gagataaaa ccgaacgaa aaagacatc ggccgcaaa 
      gtgaaactg aaacagagt cgcgcctct ctgccgccc 
      attcgtcgg gagccgccg cccccggcc cccaaaccg 
      tcttatcag ccctccaag ccggccccg ccgcccccc 
      gtcatctcc tccagcaat ggcctctcc aacggcttg 
      ggcagcaat cacgtgagc agctccagg ccccaaatg 
      aataaaccc tccgtcccc gatatcgcc cggaggccc 
      atcaaagag cgattaatt caccttctt gcgttgagg 
      ccctttaaa aaagttgaa ttacacgac cgaataacg 
      aaagagggt gtgagggag aaaaatggg ataacttcg 
      atcctcaaa cagatttcc tttatgaaa gataactgt 
      tatcacctc aatagggcg atgtggaac gaagtgaac 
      gaagactgg cctttttac accgaatcg gaacgacaa 
      atgttgaaa aggcggaag ccggcaaac ctaacccct 
      cccggcagc agcgacggc ggcagctcc ggcagcggt 
      caatccccc accagcacc caccccggt tccccccct 
      cccatttcg acgacgtca tcatcatcg aaacgtccc 
      ggttactac gacaaccag gacggtctt ccgacgaaa 
      cgtcaacgt atctcccac tatcgcaaa ccctccgaa 
      caaaattat cgttcgcca atcgaaaac aacagcagc 
      caacgtcgt ccaccgacg gattcacgt gacgccagc 
      aacatgaat ccgcgcagc agggaatcc ccgacaacg 
      gcaacggcg acggcggcg gcggcggcg gcgcctact 
      gttgccctt agcaacggc gatacgaaa ttgcgcgcc 
      gacgatgat aagcaacgt tgtgatagt catagttta 
      agctacggt ggacgtaat tcgccgtta acgaacaac 
      aaaacgacg acaacggaa aacggttac gttaacggg 
      aaatgcaca ccgagagag aaagagagg atgagagag 
      agggtgtcg agttcgagt cagaggagt gtcagggtc 
      agtccagac agccaatcg gaggcgatg gggggcggg 
      gatagggtg tcgccaatt agggaaaaa ggggcggtg 
      gctaaagtc gacagcagt tccgaattt ccggattat 
      ttaactgaa tacactacg ataaaagat gctgatcaa 
      aggcggcgt ttcaaagcc gatttcaac gccgactat 
      gccgaatat aaggacctt cacggaatc gttgaaaaa 
      gtgtctcgg aggtttgtc gatttagaa ttgaagctt 
      aagcaagaa gacgcgtct agtcccaga tttaaagat 
      ctaaaaaag caaatcgta cgagaatac aatgagaac 
      aagaacgat ctggaacac caaaaacta aaacgccgc 
      tttcaatat ctccacgac aaactgtcg cacataaaa 
      cgtctcgtc ttagagtac gaccagggt ttagccaac 
      aaccgatac tga
    mRNA sequence
    • >TC002072 RA
      cgtcagctg gttgtgaag tgcgacaga ctggactac 
      actggacag aaatggcga ctctctgtc caggtgttc 
      agtatggtt tatcatcgc agcaaaatt tcggtgaaa 
      ataaagact taatttttg tgaaactga cggattccg 
      ccgttcgtg caatatacg actttttaa aaaatcaga 
      ataaattca actgccagg gtcccacga taaagttcc 
      taggaaatg aagggcaat tatctttcc cctccgtgc 
      aaaacggct acacttcgt tcagttttt cgatgtcta 
      gtacggcgg acatggagg gccccgggg gcagtttcg 
      aatgcgtcc agcagacgg gtccccccc ggggctccc 
      tcgaagtcc tcggttcga taccataca aagtccgtg 
      tccgcgcca atgacgaca tctacgaag ccacccgtc 
      atcgtatga ccatcgccg aagaaaatc acaagaaca 
      aatgcacaa gggagataa aaccgaacg aaaaagaca 
      tcggccgca aagtgaaac tgaaacaga gtcgcgcct 
      ctctgccgc ccattcgtc gggagccgc cgcccccgg 
      cccccaaac cgtcttatc agccctcca agccggccc 
      cgccgcccc ccgtcatct cctccagca atggcctct 
      ccaacggct tgggcagca atcacgtga gcagctcca 
      ggccccaaa tgaataaac cctccgtcc ccgatatcg 
      cccggaggc ccatcaaag agcgattaa ttcaccttc 
      ttgcgttga ggcccttta aaaaagttg aattacacg 
      accgaataa cgaaagagg gtgtgaggg agaaaaatg 
      ggataactt cgatcctca aacagattt cctttatga 
      aagataact gttatcacc tcaataggg cgatgtgga 
      acgaagtga acgaagact ggccttttt acaccgaat 
      cggaacgac aaatgttga aaaggcgga agccggcaa 
      acctaaccc ctcccggca gcagcgacg gcggcagct 
      ccggcagcg gtcaatccc ccaccagca cccaccccg 
      gttcccccc ctcccattt cgacgacgt catcatcat 
      cgaaacgtc ccggttact acgacaacc aggacggtc 
      ttccgacga aacgtcaac gtatctccc actatcgca 
      aaccctccg aacaaaatt atcgttcgc caatcgaaa 
      acaacagca gccaacgtc gtccaccga cggattcac 
      gtgacgcca gcaacatga atccgcgca gcagggaat 
      ccccgacaa cggcaacgg cgacggcgg cggcggcgg 
      cggcgccta ctgttgccc ttagcaacg gcgatacga 
      aattgcgcg ccgacgatg ataagcaac gttgtgata 
      gtcatagtt taagctacg gtggacgta attcgccgt 
      taacgaaca acaaaacga cgacaacgg aaaacggtt 
      acgttaacg ggaaatgca caccgagag agaaagaga 
      ggatgagag agagggtgt cgagttcga gtcagagga 
      gtgtcaggg tcagtccag acagccaat cggaggcga 
      tggggggcg gggataggg tgtcgccaa ttagggaaa 
      aaggggcgg tggctaaag tcgacagca gttccgaat 
      ttccggatt atttaactg aatacacta cgataaaag 
      atgctgatc aaaggcggc gtttcaaag ccgatttca 
      acgccgact atgccgaat ataaggacc ttcacggaa 
      tcgttgaaa aagtgtctc ggaggtttg tcgatttag 
      aattgaagc ttaagcaag aagacgcgt ctagtccca 
      gatttaaag atctaaaaa agcaaatcg tacgagaat 
      acaatgaga acaagaacg atctggaac accaaaaac 
      taaaacgcc gctttcaat atctccacg acaaactgt 
      cgcacataa aacgtctcg tcttagagt acgaccagg 
      gtttagcca acaaccgat actgaccct gggcgcttt 
      cggcctcga ggccccacg ccccggcac attgctatg 
      gactcctgc catcggacg actcaatac taacatcac 
      gaaccacaa aaatcccat tgaaagttt aaaaactag 
      acacacaca cacacacac aaattagtc gcttcggtt 
      atttattat ttataggtt aagtggcgg ccattgctc 
      aaattgctt tgtgatacg attgattga ttgattttg 
      ttttggcac aaacgggac gccactggt aatttggtt 
      gagttcgag tgccataat aacgatccg aaaatcccc 
      aaattgaca acagtattg ccattttga ccagttacc 
      attgatctt ccgcgctct ttttttata tgtatatgt 
      tgtttcttc cgattttcc tgtgccaaa ctcctttta 
      tttattgcg ataatctca ttggacgtc cctggacgc 
      gccaactag atctaattt cgtaggcgt agaaaaaat 
      atattattg aactgtgat gaccacgct cattccgtt 
      agtgttacg ttgtccagt agcagtgcc cttcctgcc 
      aatcagact gtccgtcga tgtggttgt gattgtaca 
      tatgatctt cgatgacga cctggcctg accttgact 
      catcaaata tatatacag taccatgta tctatatta 
      catatataa ttaatatgt tagagtatg cttaatcaa 
      ctttattct gtcttctcg cttatttta tcgtttttt 
      ttccttctg agaacaaag caaaaacca aaaaaaata 
      aaaaatatt aaatgtcgt tagtccggg gttgtgtaa 
      ttctatacg taccgagtc aattgttga aaatttgcc 
      tgaatgtta tattaattg attacatga cacgtggat 
      taatgtgat aaaagtaat ggttcattg gttgtttgg 
      tttggttgg ttatattgt ttttttttg tttattgta 
      tgagtaatt ccagtgacc gtacgtaga ctaacatgt 
      tttcgagcg cattgattg ctctctttc tctctctgt 
      tgttatgta aaacgtgcc acttgttgc caagctcgg 
      tctgttgtc gatttatgt tatatatca ctgtaaata 
      agtaaaaca gtctgttta cgtttttgt ccagttcgt 
      aatcaagtg gctagggtt ttataacga cattatgtt 
      ttattgtaa attttgaac tatgcaata tatttaaaa 
      gcatccc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY