| General information | |||
| TC031632
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007425.3: 6008386..6020645 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031632 | ||
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
| Name | ||
| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03292 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%
larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head capsule decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%
head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%
affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031632 RA
MPGIFTPFL LWILSTVLA LQNEVDLIE RANIWNEPN GLMRILGRC LNNSENKDI LRTDWAYRF DENQVVLST LDLFPQGFP RDFSILTAV RLTPGDIYP LVTVYSESG EEQISVSLG NEIIFTYES TNVTFNVSL DDGEWHRLA FSIKGDSIN MIYECNNTI TQALNRSTN DLMTDGITL IGYQLTDEK FLKGDIQLL TVVPTPDAA YELCNFYAQ DCDTVVSLR EANFYAKLT NQNSLSWVV GNNASRGDL DYDEEFVTV LPTVDFVTT GRGFPGLRG FPGPSGPPG LPGAKGDSG RNGLDGVRG PVGPPGHVF MIPLNLQSN EKGPDSQSE NFRQLLSQH MMSMRGIEG PMGLTGVPG PQGPPGPEG LKGEPGDLG EPGPMGEKG LPGNPGREG RRGRPGRDG ERGITGPPG PKGEQGLLG LPGLPGDKG ERGLPGLVG EPGLRGHDG MQGEEGPPG LSGMPGEMG PRGFTGPRG LPGVPGPPG IPGNEGSAG SKGQAGPPG SPGAPGLVG PPGAVGPPG PHGLLGPPG VMGPHGKPG IPGLPGADG APGSPGSPG AMGPKGEQG LAGPAGPVG FPGPRGVKG DEGKRGILG EKGDKGDRG LEGEKGDLG MKGSVPKDL RDLMVREEK PEMLAYLGR KEKTDLRVF PDIPEIWVK KGIKGRVAK LGIMERKEI EEIRELQVN VANQDLVDS GVRVVAVAV QVNKVPRET PANLDHLDL KANEASRVL KDLGVLPEH RVLLESMEK TAIRDHPEN VDLPKTRHV GPIGEHGPP GLPGVPGEA GLPGEAGKE GAPGSPGPE GKPGPEGPT GLPGFPGER GLTGLPGIP GLKGDTGPP GAPGVTGDK GQQGEPGKD GPQGPEGRK GPPGTAGSP GAKGERGEA GPPGPSGRD GLPGLRGLP GGPGPVGPP GEDGDKGDV GPPGEKGFK GSQGETGPS GSTGPPGSR GEPGPVGPP GDKGPPGEM GRQGSKGED GPVGLPGPS GPGGLQGLP GPQGPKGEI GDMGQKGPT GAAGIPGEP GPRGTKGAD GLPGLPGPE GPPGIKGDS GTPGLPGPP GIDGKPGER GPQGQKGED GKTGLQGPP GPRGPPGTE GPKGSPGPE GFPGPPGES GLQGAKGEP GKDGEDGRQ GETGPPGPP GPVGPMGTP GLPGKTGLD GPAGLQGPS GPPGEKGDI GEIGPPGPQ GMTGPQGLP GPQGPPGLT GAPGVAGEN GAPGSPGLT GSPGKTGSP GPAGQKGDR GRRGPKGHR GEMGLSGIK GDLGEKGNK GDKGPPGPE GRKGETGPE GPIGIKGSD GPAGPPGME GPPGPKGVE GQTGPKGEP GPPGPAGPP GPPAEMPLL PPEMLFERT FSRERRNVE EIISEYEDM NTEAETDDN GKFLEMYNT IYSMRQDLE RVKKPIGTR ENPVRSCKD LFYGHPQFP DGWYWIDPN SGMTDDAIY VYCNMTAEG ETCVFPDAH SANMPNIPW RQEGGKKDW YSHLRGGFK ITYETVGVV QMTFLRLLY QEAYQNFTF SCINTIAWF NENTQDHKM ALKFRGDNE YEFAHKKTG LKVIQDGCQ DGTTKSETI FELRTTKLD RLPIVDFFP LDYGAPNQA FGFTVGPIC LK
- >TC031632 RA
atgcccgga attttcaca ccattcctc ctatggatc ctctccacc gtcttagcg ctacaaaac gaagtggat ctaatcgag agagctaac atttggaac gaaccaaac ggacttatg cgcatttta ggccgatgt ttgaacaat tccgaaaat aaagacatt ctacgaacg gattgggct tacagattt gacgaaaat caagtggtc ttaagcaca cttgattta tttccgcag gggtttccc cgggatttt tccattttg acagctgtc agactcaca cctggcgac atctatccg ctagtcact gtatacagt gaaagcggg gaagaacag atttctgtt tcactagga aatgaaatt attttcacc tacgaaagc actaatgtg acctttaac gtatcacta gatgatgga gaatggcac cgactagct tttagcatc aaaggcgac tcgattaat atgatatat gagtgcaat aacaccata acccaggct ctaaaccgc tcaaccaat gatttaatg acagatggc atcactctg attggttac caactgaca gacgagaaa ttcctcaag ggtgatatt caactcctg acagtggta ccaacccca gacgccgcc tacgaattg tgcaacttt tatgcacaa gattgtgac acggttgta agtcttcga gaggccaat ttttacgcc aagttaaca aaccaaaat tccctaagt tgggttgtt ggcaacaat gcaagtcgt ggcgatctt gattacgac gaggaattt gttacagtg ttgccaacc gttgatttt gtgacaacc gggcgcggt tttccgggt ctgaggggt tttccggga ccttcggga ccccccgga cttccgggt gccaaaggt gactccgga aggaacggt ttggacggg gtgaggggt ccggttggc ccccctggg catgtcttt atgattccg ttaaatttg caaagtaat gaaaaaggg ccggactcc cagtctgag aacttccgc cagttgttg tcgcaacat atgatgtcg atgcgtggg attgaaggt ccgatggga ttaacggga gttcccggt cctcaaggt cccccaggt cccgaggga ttaaagggg gaacctggg gatctgggt gaaccggga cctatgggt gaaaaaggc cttccggga aatcccgga cgtgaaggg agacgtgga agaccaggc cgtgatggg gaacgtggg attacaggc cctccgggt cctaaaggg gaacaggga ttattggga ttaccggga cttccgggt gataaaggg gagaggggg ctcccgggt ttggtcggc gaaccggga ttacgtggt cacgacggg atgcaaggc gaggaaggt cctccggga ctttcggga atgcctgga gaaatgggc cctcggggg ttcaccggg cctagggga ttacccgga gtgccaggc ccgcccgga attccgggt aatgaagga tcggcgggt tccaaaggc caggcggga cctcccgga tcacctgga gcaccggga ttagttgga ccccctgga gcggtgggt cctccaggt cctcacggg ttacttgga ccaccggga gttatggga ccgcatggg aaacccggg attccggga ttaccgggt gcagatggc gcacccgga agtcccggg agtcccgga gcaatgggt cctaaagga gagcaaggt ttggcggga ccggcgggt cctgtgggt tttccggga ccacgtggt gttaagggg gatgaaggg aaaaggggg attctgggt gaaaaaggg gataagggt gataggggt ttggagggg gaaaaaggg gatttgggg atgaaggga agtgtcccg aaggaccta agggatttg atggtccga gaggagaaa ccggaaatg ttggcttac ctggggaga aaggaaaag acggacctc cgggtcttc ccggatatc ccggaaatc tgggtgaaa aaggggata aagggtcga gtggcaaaa ttggggata atggagaga aaggagata gaggaaatc cgggaactc caggtgaac gtggcgaac caggacctc gtggattcc ggggttcgc gtggtcgcc gtggccgtg caggtcaac aaggtccca agggagaca ccggccaac ctggaccac ctggacctc aaggcgaac gaggcatcc cgggtcctg aaggacctc ggggttttg ccggagcac cgggtcctc ctggaatca atggaaaag acggccatc cgggatcac ccggagaac gtggacctc ccgaaaacc cggcatgtt ggtccaatt ggggaacat ggacctccc ggacttccg ggggttccg ggtgaggct ggtctgcct ggagaagca gggaaagag ggagctcca ggaagtcct ggaccggag gggaaacca ggtcctgaa ggcccaact ggattacct ggttttccg ggtgaacgg ggattgacg ggattaccg ggaattccg ggtcttaaa ggcgatacg ggaccgcct ggagctccg ggagttacg ggagataag gggcagcag ggagaaccg gggaaggat ggaccacaa ggtcccgaa ggtcggaaa ggtcctcca ggaacagcg ggatccccc ggtgctaaa ggggaacgt ggcgaggct ggacctccc ggtccgtca ggacgtgat ggtttaccg ggactcaga gggttacct ggaggtcca ggtcctgta ggacccccg ggtgaagat ggcgataag ggcgatgtt ggtccacct ggggaaaag ggatttaaa ggaagtcaa ggagaaacg gggccgtct ggaagtacc ggtcctcct ggatcaaga ggggaacca ggtcccgtc ggaccccca ggtgataaa ggaccacca ggtgaaatg ggacgtcaa gggtcaaag ggtgaggat ggtcccgtg ggactcccg ggaccttcc ggacctgga ggattacaa gggttaccc ggacctcaa ggtcctaaa ggagaaata ggagatatg ggtcagaaa ggtcccaca ggagcagca ggaataccg ggagaaccg ggtcctaga ggaacgaaa ggagccgat ggtttaccc ggattgcct ggacccgaa ggtcctccg ggaattaaa ggcgattcg ggaactcct ggactacca ggacctcca gggattgat ggaaaaccg ggagaaagg ggaccacaa ggacaaaaa ggcgaagac ggaaaaacg gggcttcaa gggcctcct ggcccgaga ggtcccccg ggaacagaa ggtccaaaa ggatcacca ggacctgaa ggttttccc ggccccccg ggcgaatca ggccttcaa ggagctaaa ggcgaacct ggaaaagac ggtgaagac ggtcgtcaa ggtgaaact ggaccccca ggaccccca ggtcctgta ggtcccatg ggaacaccg ggacttccg ggaaaaacg ggacttgac ggtcctgca ggacttcaa ggaccttcg ggacccccg ggtgaaaaa ggcgacatc ggcgaaatc ggccctcca ggtcctcaa ggaatgaca ggacctcaa ggcctacct ggccctcaa ggacctccc ggattaaca ggagctcca ggcgttgct ggggaaaat ggagctcca ggttctcca ggcttaaca ggatcacct ggaaaaacc ggaagtccg ggtcctgca ggacagaag ggagaccgg ggaaggaga ggtccgaaa ggacaccga ggcgaaatg ggattatcg ggaattaaa ggggatttg ggcgagaag gggaataaa ggggataag ggacctcca ggtcctgaa ggacgtaaa ggagagaca ggacctgaa ggtcccatc ggaattaaa ggaagtgat ggtcctgcg ggacctcca ggaatggaa ggaccaccg ggacctaaa ggagtggaa ggtcagacg ggaccaaag ggcgaacct ggaccccca ggtcctgca ggaccacct ggacctccg gcggaaatg ccgctactt ccaccggaa atgctcttc gaacgaact tttagtcgg gaaaggcgc aatgttgag gaaattatt tccgaatat gaagatatg aacacggaa gcggaaaca gacgataat ggaaaattc ctggaaatg tacaacaca atatattcc atgagacag gacttagaa agggtgaaa aagcctata gggacccga gaaaaccca gtccggtcc tgcaaagac ttgttttac ggccaccct caattccct gatggatgg tactggatt gaccccaac tcgggtatg accgacgac gccatctat gtttattgc aacatgaca gctgagggc gaaacgtgc gtttttccg gacgcccac agcgccaac atgcccaac ataccctgg agacaagaa gggggcaaa aaagattgg tactcccac ctacgcggc ggctttaaa ataacttat gaaacagta ggcgtcgtc caaatgaca ttcctccgc cttctgtac caagaagcc taccaaaat ttcaccttt tcttgcata aacaccatc gcatggttt aacgaaaac acgcaagac cacaaaatg gctcttaaa ttccgcggc gacaacgag tacgaattt gcgcacaag aaaaccggt ctgaaagtt atccaagat ggttgccaa gatggcacc accaaaagt gagacaatt ttcgagtta cggaccaca aaattggac cgtttgccc atcgttgac ttttttccg cttgactat ggcgccccc aaccaagcg tttgggttc acagtgggc cccatttgc ctcaagtag
- >TC031632 RA
ttccactta agagccagt gtgcgagtg tgctgtggc atggagtga gcaagtttt gaactaatt agtgccatc gacccaagc gatgattta tcggacaaa atgcccgga attttcaca ccattcctc ctatggatc ctctccacc gtcttagcg ctacaaaac gaagtggat ctaatcgag agagctaac atttggaac gaaccaaac ggacttatg cgcatttta ggccgatgt ttgaacaat tccgaaaat aaagacatt ctacgaacg gattgggct tacagattt gacgaaaat caagtggtc ttaagcaca cttgattta tttccgcag gggtttccc cgggatttt tccattttg acagctgtc agactcaca cctggcgac atctatccg ctagtcact gtatacagt gaaagcggg gaagaacag atttctgtt tcactagga aatgaaatt attttcacc tacgaaagc actaatgtg acctttaac gtatcacta gatgatgga gaatggcac cgactagct tttagcatc aaaggcgac tcgattaat atgatatat gagtgcaat aacaccata acccaggct ctaaaccgc tcaaccaat gatttaatg acagatggc atcactctg attggttac caactgaca gacgagaaa ttcctcaag ggtgatatt caactcctg acagtggta ccaacccca gacgccgcc tacgaattg tgcaacttt tatgcacaa gattgtgac acggttgta agtcttcga gaggccaat ttttacgcc aagttaaca aaccaaaat tccctaagt tgggttgtt ggcaacaat gcaagtcgt ggcgatctt gattacgac gaggaattt gttacagtg ttgccaacc gttgatttt gtgacaacc gggcgcggt tttccgggt ctgaggggt tttccggga ccttcggga ccccccgga cttccgggt gccaaaggt gactccgga aggaacggt ttggacggg gtgaggggt ccggttggc ccccctggg catgtcttt atgattccg ttaaatttg caaagtaat gaaaaaggg ccggactcc cagtctgag aacttccgc cagttgttg tcgcaacat atgatgtcg atgcgtggg attgaaggt ccgatggga ttaacggga gttcccggt cctcaaggt cccccaggt cccgaggga ttaaagggg gaacctggg gatctgggt gaaccggga cctatgggt gaaaaaggc cttccggga aatcccgga cgtgaaggg agacgtgga agaccaggc cgtgatggg gaacgtggg attacaggc cctccgggt cctaaaggg gaacaggga ttattggga ttaccggga cttccgggt gataaaggg gagaggggg ctcccgggt ttggtcggc gaaccggga ttacgtggt cacgacggg atgcaaggc gaggaaggt cctccggga ctttcggga atgcctgga gaaatgggc cctcggggg ttcaccggg cctagggga ttacccgga gtgccaggc ccgcccgga attccgggt aatgaagga tcggcgggt tccaaaggc caggcggga cctcccgga tcacctgga gcaccggga ttagttgga ccccctgga gcggtgggt cctccaggt cctcacggg ttacttgga ccaccggga gttatggga ccgcatggg aaacccggg attccggga ttaccgggt gcagatggc gcacccgga agtcccggg agtcccgga gcaatgggt cctaaagga gagcaaggt ttggcggga ccggcgggt cctgtgggt tttccggga ccacgtggt gttaagggg gatgaaggg aaaaggggg attctgggt gaaaaaggg gataagggt gataggggt ttggagggg gaaaaaggg gatttgggg atgaaggga agtgtcccg aaggaccta agggatttg atggtccga gaggagaaa ccggaaatg ttggcttac ctggggaga aaggaaaag acggacctc cgggtcttc ccggatatc ccggaaatc tgggtgaaa aaggggata aagggtcga gtggcaaaa ttggggata atggagaga aaggagata gaggaaatc cgggaactc caggtgaac gtggcgaac caggacctc gtggattcc ggggttcgc gtggtcgcc gtggccgtg caggtcaac aaggtccca agggagaca ccggccaac ctggaccac ctggacctc aaggcgaac gaggcatcc cgggtcctg aaggacctc ggggttttg ccggagcac cgggtcctc ctggaatca atggaaaag acggccatc cgggatcac ccggagaac gtggacctc ccgaaaacc cggcatgtt ggtccaatt ggggaacat ggacctccc ggacttccg ggggttccg ggtgaggct ggtctgcct ggagaagca gggaaagag ggagctcca ggaagtcct ggaccggag gggaaacca ggtcctgaa ggcccaact ggattacct ggttttccg ggtgaacgg ggattgacg ggattaccg ggaattccg ggtcttaaa ggcgatacg ggaccgcct ggagctccg ggagttacg ggagataag gggcagcag ggagaaccg gggaaggat ggaccacaa ggtcccgaa ggtcggaaa ggtcctcca ggaacagcg ggatccccc ggtgctaaa ggggaacgt ggcgaggct ggacctccc ggtccgtca ggacgtgat ggtttaccg ggactcaga gggttacct ggaggtcca ggtcctgta ggacccccg ggtgaagat ggcgataag ggcgatgtt ggtccacct ggggaaaag ggatttaaa ggaagtcaa ggagaaacg gggccgtct ggaagtacc ggtcctcct ggatcaaga ggggaacca ggtcccgtc ggaccccca ggtgataaa ggaccacca ggtgaaatg ggacgtcaa gggtcaaag ggtgaggat ggtcccgtg ggactcccg ggaccttcc ggacctgga ggattacaa gggttaccc ggacctcaa ggtcctaaa ggagaaata ggagatatg ggtcagaaa ggtcccaca ggagcagca ggaataccg ggagaaccg ggtcctaga ggaacgaaa ggagccgat ggtttaccc ggattgcct ggacccgaa ggtcctccg ggaattaaa ggcgattcg ggaactcct ggactacca ggacctcca gggattgat ggaaaaccg ggagaaagg ggaccacaa ggacaaaaa ggcgaagac ggaaaaacg gggcttcaa gggcctcct ggcccgaga ggtcccccg ggaacagaa ggtccaaaa ggatcacca ggacctgaa ggttttccc ggccccccg ggcgaatca ggccttcaa ggagctaaa ggcgaacct ggaaaagac ggtgaagac ggtcgtcaa ggtgaaact ggaccccca ggaccccca ggtcctgta ggtcccatg ggaacaccg ggacttccg ggaaaaacg ggacttgac ggtcctgca ggacttcaa ggaccttcg ggacccccg ggtgaaaaa ggcgacatc ggcgaaatc ggccctcca ggtcctcaa ggaatgaca ggacctcaa ggcctacct ggccctcaa ggacctccc ggattaaca ggagctcca ggcgttgct ggggaaaat ggagctcca ggttctcca ggcttaaca ggatcacct ggaaaaacc ggaagtccg ggtcctgca ggacagaag ggagaccgg ggaaggaga ggtccgaaa ggacaccga ggcgaaatg ggattatcg ggaattaaa ggggatttg ggcgagaag gggaataaa ggggataag ggacctcca ggtcctgaa ggacgtaaa ggagagaca ggacctgaa ggtcccatc ggaattaaa ggaagtgat ggtcctgcg ggacctcca ggaatggaa ggaccaccg ggacctaaa ggagtggaa ggtcagacg ggaccaaag ggcgaacct ggaccccca ggtcctgca ggaccacct ggacctccg gcggaaatg ccgctactt ccaccggaa atgctcttc gaacgaact tttagtcgg gaaaggcgc aatgttgag gaaattatt tccgaatat gaagatatg aacacggaa gcggaaaca gacgataat ggaaaattc ctggaaatg tacaacaca atatattcc atgagacag gacttagaa agggtgaaa aagcctata gggacccga gaaaaccca gtccggtcc tgcaaagac ttgttttac ggccaccct caattccct gatggatgg tactggatt gaccccaac tcgggtatg accgacgac gccatctat gtttattgc aacatgaca gctgagggc gaaacgtgc gtttttccg gacgcccac agcgccaac atgcccaac ataccctgg agacaagaa gggggcaaa aaagattgg tactcccac ctacgcggc ggctttaaa ataacttat gaaacagta ggcgtcgtc caaatgaca ttcctccgc cttctgtac caagaagcc taccaaaat ttcaccttt tcttgcata aacaccatc gcatggttt aacgaaaac acgcaagac cacaaaatg gctcttaaa ttccgcggc gacaacgag tacgaattt gcgcacaag aaaaccggt ctgaaagtt atccaagat ggttgccaa gatggcacc accaaaagt gagacaatt ttcgagtta cggaccaca aaattggac cgtttgccc atcgttgac ttttttccg cttgactat ggcgccccc aaccaagcg tttgggttc acagtgggc cccatttgc ctcaagtag attctaacc caagttttg tcatagcac cttatttat gaatttact taggaaatg taaatattt tgcaacact gccaacttc catatcgtg tgtacatac atttttaaa taaattttt aaagctctg
