Status
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Gene information
General information
TC031632
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002063,TC002064
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid75: 1705705..1717964
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031632
    iBeetle screen iB_03292
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    cuticle remnants with some identifiable segments - Penetrance (percentage of animals/eggs on slide): less than 30%

    larva everted (inside-out) - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head capsule decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head appendages random not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031632 RA
      MPGIFTPFL LWILSTVLA LQNEVDLIE RANIWNEPN 
      GLMRILGRC LNNSENKDI LRTDWAYRF DENQVVLST 
      LDLFPQGFP RDFSILTAV RLTPGDIYP LVTVYSESG 
      EEQISVSLG NEIIFTYES TNVTFNVSL DDGEWHRLA 
      FSIKGDSIN MIYECNNTI TQALNRSTN DLMTDGITL 
      IGYQLTDEK FLKGDIQLL TVVPTPDAA YELCNFYAQ 
      DCDTVVSLR EANFYAKLT NQNSLSWVV GNNASRGDL 
      DYDEEFVTV LPTVDFVTT GRGFPGLRG FPGPSGPPG 
      LPGAKGDSG RNGLDGVRG PVGPPGHVF MIPLNLQSN 
      EKGPDSQSE NFRQLLSQH MMSMRGIEG PMGLTGVPG 
      PQGPPGPEG LKGEPGDLG EPGPMGEKG LPGNPGREG 
      RRGRPGRDG ERGITGPPG PKGEQGLLG LPGLPGDKG 
      ERGLPGLVG EPGLRGHDG MQGEEGPPG LSGMPGEMG 
      PRGFTGPRG LPGVPGPPG IPGNEGSAG SKGQAGPPG 
      SPGAPGLVG PPGAVGPPG PHGLLGPPG VMGPHGKPG 
      IPGLPGADG APGSPGSPG AMGPKGEQG LAGPAGPVG 
      FPGPRGVKG DEGKRGILG EKGDKGDRG LEGEKGDLG 
      MKGSVPKDL RDLMVREEK PEMLAYLGR KEKTDLRVF 
      PDIPEIWVK KGIKGRVAK LGIMERKEI EEIRELQVN 
      VANQDLVDS GVRVVAVAV QVNKVPRET PANLDHLDL 
      KANEASRVL KDLGVLPEH RVLLESMEK TAIRDHPEN 
      VDLPKTRHV GPIGEHGPP GLPGVPGEA GLPGEAGKE 
      GAPGSPGPE GKPGPEGPT GLPGFPGER GLTGLPGIP 
      GLKGDTGPP GAPGVTGDK GQQGEPGKD GPQGPEGRK 
      GPPGTAGSP GAKGERGEA GPPGPSGRD GLPGLRGLP 
      GGPGPVGPP GEDGDKGDV GPPGEKGFK GSQGETGPS 
      GSTGPPGSR GEPGPVGPP GDKGPPGEM GRQGSKGED 
      GPVGLPGPS GPGGLQGLP GPQGPKGEI GDMGQKGPT 
      GAAGIPGEP GPRGTKGAD GLPGLPGPE GPPGIKGDS 
      GTPGLPGPP GIDGKPGER GPQGQKGED GKTGLQGPP 
      GPRGPPGTE GPKGSPGPE GFPGPPGES GLQGAKGEP 
      GKDGEDGRQ GETGPPGPP GPVGPMGTP GLPGKTGLD 
      GPAGLQGPS GPPGEKGDI GEIGPPGPQ GMTGPQGLP 
      GPQGPPGLT GAPGVAGEN GAPGSPGLT GSPGKTGSP 
      GPAGQKGDR GRRGPKGHR GEMGLSGIK GDLGEKGNK 
      GDKGPPGPE GRKGETGPE GPIGIKGSD GPAGPPGME 
      GPPGPKGVE GQTGPKGEP GPPGPAGPP GPPAEMPLL 
      PPEMLFERT FSRERRNVE EIISEYEDM NTEAETDDN 
      GKFLEMYNT IYSMRQDLE RVKKPIGTR ENPVRSCKD 
      LFYGHPQFP DGWYWIDPN SGMTDDAIY VYCNMTAEG 
      ETCVFPDAH SANMPNIPW RQEGGKKDW YSHLRGGFK 
      ITYETVGVV QMTFLRLLY QEAYQNFTF SCINTIAWF 
      NENTQDHKM ALKFRGDNE YEFAHKKTG LKVIQDGCQ 
      DGTTKSETI FELRTTKLD RLPIVDFFP LDYGAPNQA 
      FGFTVGPIC LK
    CDS sequence
    • >TC031632 RA
      atgcccgga attttcaca ccattcctc ctatggatc 
      ctctccacc gtcttagcg ctacaaaac gaagtggat 
      ctaatcgag agagctaac atttggaac gaaccaaac 
      ggacttatg cgcatttta ggccgatgt ttgaacaat 
      tccgaaaat aaagacatt ctacgaacg gattgggct 
      tacagattt gacgaaaat caagtggtc ttaagcaca 
      cttgattta tttccgcag gggtttccc cgggatttt 
      tccattttg acagctgtc agactcaca cctggcgac 
      atctatccg ctagtcact gtatacagt gaaagcggg 
      gaagaacag atttctgtt tcactagga aatgaaatt 
      attttcacc tacgaaagc actaatgtg acctttaac 
      gtatcacta gatgatgga gaatggcac cgactagct 
      tttagcatc aaaggcgac tcgattaat atgatatat 
      gagtgcaat aacaccata acccaggct ctaaaccgc 
      tcaaccaat gatttaatg acagatggc atcactctg 
      attggttac caactgaca gacgagaaa ttcctcaag 
      ggtgatatt caactcctg acagtggta ccaacccca 
      gacgccgcc tacgaattg tgcaacttt tatgcacaa 
      gattgtgac acggttgta agtcttcga gaggccaat 
      ttttacgcc aagttaaca aaccaaaat tccctaagt 
      tgggttgtt ggcaacaat gcaagtcgt ggcgatctt 
      gattacgac gaggaattt gttacagtg ttgccaacc 
      gttgatttt gtgacaacc gggcgcggt tttccgggt 
      ctgaggggt tttccggga ccttcggga ccccccgga 
      cttccgggt gccaaaggt gactccgga aggaacggt 
      ttggacggg gtgaggggt ccggttggc ccccctggg 
      catgtcttt atgattccg ttaaatttg caaagtaat 
      gaaaaaggg ccggactcc cagtctgag aacttccgc 
      cagttgttg tcgcaacat atgatgtcg atgcgtggg 
      attgaaggt ccgatggga ttaacggga gttcccggt 
      cctcaaggt cccccaggt cccgaggga ttaaagggg 
      gaacctggg gatctgggt gaaccggga cctatgggt 
      gaaaaaggc cttccggga aatcccgga cgtgaaggg 
      agacgtgga agaccaggc cgtgatggg gaacgtggg 
      attacaggc cctccgggt cctaaaggg gaacaggga 
      ttattggga ttaccggga cttccgggt gataaaggg 
      gagaggggg ctcccgggt ttggtcggc gaaccggga 
      ttacgtggt cacgacggg atgcaaggc gaggaaggt 
      cctccggga ctttcggga atgcctgga gaaatgggc 
      cctcggggg ttcaccggg cctagggga ttacccgga 
      gtgccaggc ccgcccgga attccgggt aatgaagga 
      tcggcgggt tccaaaggc caggcggga cctcccgga 
      tcacctgga gcaccggga ttagttgga ccccctgga 
      gcggtgggt cctccaggt cctcacggg ttacttgga 
      ccaccggga gttatggga ccgcatggg aaacccggg 
      attccggga ttaccgggt gcagatggc gcacccgga 
      agtcccggg agtcccgga gcaatgggt cctaaagga 
      gagcaaggt ttggcggga ccggcgggt cctgtgggt 
      tttccggga ccacgtggt gttaagggg gatgaaggg 
      aaaaggggg attctgggt gaaaaaggg gataagggt 
      gataggggt ttggagggg gaaaaaggg gatttgggg 
      atgaaggga agtgtcccg aaggaccta agggatttg 
      atggtccga gaggagaaa ccggaaatg ttggcttac 
      ctggggaga aaggaaaag acggacctc cgggtcttc 
      ccggatatc ccggaaatc tgggtgaaa aaggggata 
      aagggtcga gtggcaaaa ttggggata atggagaga 
      aaggagata gaggaaatc cgggaactc caggtgaac 
      gtggcgaac caggacctc gtggattcc ggggttcgc 
      gtggtcgcc gtggccgtg caggtcaac aaggtccca 
      agggagaca ccggccaac ctggaccac ctggacctc 
      aaggcgaac gaggcatcc cgggtcctg aaggacctc 
      ggggttttg ccggagcac cgggtcctc ctggaatca 
      atggaaaag acggccatc cgggatcac ccggagaac 
      gtggacctc ccgaaaacc cggcatgtt ggtccaatt 
      ggggaacat ggacctccc ggacttccg ggggttccg 
      ggtgaggct ggtctgcct ggagaagca gggaaagag 
      ggagctcca ggaagtcct ggaccggag gggaaacca 
      ggtcctgaa ggcccaact ggattacct ggttttccg 
      ggtgaacgg ggattgacg ggattaccg ggaattccg 
      ggtcttaaa ggcgatacg ggaccgcct ggagctccg 
      ggagttacg ggagataag gggcagcag ggagaaccg 
      gggaaggat ggaccacaa ggtcccgaa ggtcggaaa 
      ggtcctcca ggaacagcg ggatccccc ggtgctaaa 
      ggggaacgt ggcgaggct ggacctccc ggtccgtca 
      ggacgtgat ggtttaccg ggactcaga gggttacct 
      ggaggtcca ggtcctgta ggacccccg ggtgaagat 
      ggcgataag ggcgatgtt ggtccacct ggggaaaag 
      ggatttaaa ggaagtcaa ggagaaacg gggccgtct 
      ggaagtacc ggtcctcct ggatcaaga ggggaacca 
      ggtcccgtc ggaccccca ggtgataaa ggaccacca 
      ggtgaaatg ggacgtcaa gggtcaaag ggtgaggat 
      ggtcccgtg ggactcccg ggaccttcc ggacctgga 
      ggattacaa gggttaccc ggacctcaa ggtcctaaa 
      ggagaaata ggagatatg ggtcagaaa ggtcccaca 
      ggagcagca ggaataccg ggagaaccg ggtcctaga 
      ggaacgaaa ggagccgat ggtttaccc ggattgcct 
      ggacccgaa ggtcctccg ggaattaaa ggcgattcg 
      ggaactcct ggactacca ggacctcca gggattgat 
      ggaaaaccg ggagaaagg ggaccacaa ggacaaaaa 
      ggcgaagac ggaaaaacg gggcttcaa gggcctcct 
      ggcccgaga ggtcccccg ggaacagaa ggtccaaaa 
      ggatcacca ggacctgaa ggttttccc ggccccccg 
      ggcgaatca ggccttcaa ggagctaaa ggcgaacct 
      ggaaaagac ggtgaagac ggtcgtcaa ggtgaaact 
      ggaccccca ggaccccca ggtcctgta ggtcccatg 
      ggaacaccg ggacttccg ggaaaaacg ggacttgac 
      ggtcctgca ggacttcaa ggaccttcg ggacccccg 
      ggtgaaaaa ggcgacatc ggcgaaatc ggccctcca 
      ggtcctcaa ggaatgaca ggacctcaa ggcctacct 
      ggccctcaa ggacctccc ggattaaca ggagctcca 
      ggcgttgct ggggaaaat ggagctcca ggttctcca 
      ggcttaaca ggatcacct ggaaaaacc ggaagtccg 
      ggtcctgca ggacagaag ggagaccgg ggaaggaga 
      ggtccgaaa ggacaccga ggcgaaatg ggattatcg 
      ggaattaaa ggggatttg ggcgagaag gggaataaa 
      ggggataag ggacctcca ggtcctgaa ggacgtaaa 
      ggagagaca ggacctgaa ggtcccatc ggaattaaa 
      ggaagtgat ggtcctgcg ggacctcca ggaatggaa 
      ggaccaccg ggacctaaa ggagtggaa ggtcagacg 
      ggaccaaag ggcgaacct ggaccccca ggtcctgca 
      ggaccacct ggacctccg gcggaaatg ccgctactt 
      ccaccggaa atgctcttc gaacgaact tttagtcgg 
      gaaaggcgc aatgttgag gaaattatt tccgaatat 
      gaagatatg aacacggaa gcggaaaca gacgataat 
      ggaaaattc ctggaaatg tacaacaca atatattcc 
      atgagacag gacttagaa agggtgaaa aagcctata 
      gggacccga gaaaaccca gtccggtcc tgcaaagac 
      ttgttttac ggccaccct caattccct gatggatgg 
      tactggatt gaccccaac tcgggtatg accgacgac 
      gccatctat gtttattgc aacatgaca gctgagggc 
      gaaacgtgc gtttttccg gacgcccac agcgccaac 
      atgcccaac ataccctgg agacaagaa gggggcaaa 
      aaagattgg tactcccac ctacgcggc ggctttaaa 
      ataacttat gaaacagta ggcgtcgtc caaatgaca 
      ttcctccgc cttctgtac caagaagcc taccaaaat 
      ttcaccttt tcttgcata aacaccatc gcatggttt 
      aacgaaaac acgcaagac cacaaaatg gctcttaaa 
      ttccgcggc gacaacgag tacgaattt gcgcacaag 
      aaaaccggt ctgaaagtt atccaagat ggttgccaa 
      gatggcacc accaaaagt gagacaatt ttcgagtta 
      cggaccaca aaattggac cgtttgccc atcgttgac 
      ttttttccg cttgactat ggcgccccc aaccaagcg 
      tttgggttc acagtgggc cccatttgc ctcaagtag
    mRNA sequence
    • >TC031632 RA
      ttccactta agagccagt gtgcgagtg tgctgtggc 
      atggagtga gcaagtttt gaactaatt agtgccatc 
      gacccaagc gatgattta tcggacaaa atgcccgga 
      attttcaca ccattcctc ctatggatc ctctccacc 
      gtcttagcg ctacaaaac gaagtggat ctaatcgag 
      agagctaac atttggaac gaaccaaac ggacttatg 
      cgcatttta ggccgatgt ttgaacaat tccgaaaat 
      aaagacatt ctacgaacg gattgggct tacagattt 
      gacgaaaat caagtggtc ttaagcaca cttgattta 
      tttccgcag gggtttccc cgggatttt tccattttg 
      acagctgtc agactcaca cctggcgac atctatccg 
      ctagtcact gtatacagt gaaagcggg gaagaacag 
      atttctgtt tcactagga aatgaaatt attttcacc 
      tacgaaagc actaatgtg acctttaac gtatcacta 
      gatgatgga gaatggcac cgactagct tttagcatc 
      aaaggcgac tcgattaat atgatatat gagtgcaat 
      aacaccata acccaggct ctaaaccgc tcaaccaat 
      gatttaatg acagatggc atcactctg attggttac 
      caactgaca gacgagaaa ttcctcaag ggtgatatt 
      caactcctg acagtggta ccaacccca gacgccgcc 
      tacgaattg tgcaacttt tatgcacaa gattgtgac 
      acggttgta agtcttcga gaggccaat ttttacgcc 
      aagttaaca aaccaaaat tccctaagt tgggttgtt 
      ggcaacaat gcaagtcgt ggcgatctt gattacgac 
      gaggaattt gttacagtg ttgccaacc gttgatttt 
      gtgacaacc gggcgcggt tttccgggt ctgaggggt 
      tttccggga ccttcggga ccccccgga cttccgggt 
      gccaaaggt gactccgga aggaacggt ttggacggg 
      gtgaggggt ccggttggc ccccctggg catgtcttt 
      atgattccg ttaaatttg caaagtaat gaaaaaggg 
      ccggactcc cagtctgag aacttccgc cagttgttg 
      tcgcaacat atgatgtcg atgcgtggg attgaaggt 
      ccgatggga ttaacggga gttcccggt cctcaaggt 
      cccccaggt cccgaggga ttaaagggg gaacctggg 
      gatctgggt gaaccggga cctatgggt gaaaaaggc 
      cttccggga aatcccgga cgtgaaggg agacgtgga 
      agaccaggc cgtgatggg gaacgtggg attacaggc 
      cctccgggt cctaaaggg gaacaggga ttattggga 
      ttaccggga cttccgggt gataaaggg gagaggggg 
      ctcccgggt ttggtcggc gaaccggga ttacgtggt 
      cacgacggg atgcaaggc gaggaaggt cctccggga 
      ctttcggga atgcctgga gaaatgggc cctcggggg 
      ttcaccggg cctagggga ttacccgga gtgccaggc 
      ccgcccgga attccgggt aatgaagga tcggcgggt 
      tccaaaggc caggcggga cctcccgga tcacctgga 
      gcaccggga ttagttgga ccccctgga gcggtgggt 
      cctccaggt cctcacggg ttacttgga ccaccggga 
      gttatggga ccgcatggg aaacccggg attccggga 
      ttaccgggt gcagatggc gcacccgga agtcccggg 
      agtcccgga gcaatgggt cctaaagga gagcaaggt 
      ttggcggga ccggcgggt cctgtgggt tttccggga 
      ccacgtggt gttaagggg gatgaaggg aaaaggggg 
      attctgggt gaaaaaggg gataagggt gataggggt 
      ttggagggg gaaaaaggg gatttgggg atgaaggga 
      agtgtcccg aaggaccta agggatttg atggtccga 
      gaggagaaa ccggaaatg ttggcttac ctggggaga 
      aaggaaaag acggacctc cgggtcttc ccggatatc 
      ccggaaatc tgggtgaaa aaggggata aagggtcga 
      gtggcaaaa ttggggata atggagaga aaggagata 
      gaggaaatc cgggaactc caggtgaac gtggcgaac 
      caggacctc gtggattcc ggggttcgc gtggtcgcc 
      gtggccgtg caggtcaac aaggtccca agggagaca 
      ccggccaac ctggaccac ctggacctc aaggcgaac 
      gaggcatcc cgggtcctg aaggacctc ggggttttg 
      ccggagcac cgggtcctc ctggaatca atggaaaag 
      acggccatc cgggatcac ccggagaac gtggacctc 
      ccgaaaacc cggcatgtt ggtccaatt ggggaacat 
      ggacctccc ggacttccg ggggttccg ggtgaggct 
      ggtctgcct ggagaagca gggaaagag ggagctcca 
      ggaagtcct ggaccggag gggaaacca ggtcctgaa 
      ggcccaact ggattacct ggttttccg ggtgaacgg 
      ggattgacg ggattaccg ggaattccg ggtcttaaa 
      ggcgatacg ggaccgcct ggagctccg ggagttacg 
      ggagataag gggcagcag ggagaaccg gggaaggat 
      ggaccacaa ggtcccgaa ggtcggaaa ggtcctcca 
      ggaacagcg ggatccccc ggtgctaaa ggggaacgt 
      ggcgaggct ggacctccc ggtccgtca ggacgtgat 
      ggtttaccg ggactcaga gggttacct ggaggtcca 
      ggtcctgta ggacccccg ggtgaagat ggcgataag 
      ggcgatgtt ggtccacct ggggaaaag ggatttaaa 
      ggaagtcaa ggagaaacg gggccgtct ggaagtacc 
      ggtcctcct ggatcaaga ggggaacca ggtcccgtc 
      ggaccccca ggtgataaa ggaccacca ggtgaaatg 
      ggacgtcaa gggtcaaag ggtgaggat ggtcccgtg 
      ggactcccg ggaccttcc ggacctgga ggattacaa 
      gggttaccc ggacctcaa ggtcctaaa ggagaaata 
      ggagatatg ggtcagaaa ggtcccaca ggagcagca 
      ggaataccg ggagaaccg ggtcctaga ggaacgaaa 
      ggagccgat ggtttaccc ggattgcct ggacccgaa 
      ggtcctccg ggaattaaa ggcgattcg ggaactcct 
      ggactacca ggacctcca gggattgat ggaaaaccg 
      ggagaaagg ggaccacaa ggacaaaaa ggcgaagac 
      ggaaaaacg gggcttcaa gggcctcct ggcccgaga 
      ggtcccccg ggaacagaa ggtccaaaa ggatcacca 
      ggacctgaa ggttttccc ggccccccg ggcgaatca 
      ggccttcaa ggagctaaa ggcgaacct ggaaaagac 
      ggtgaagac ggtcgtcaa ggtgaaact ggaccccca 
      ggaccccca ggtcctgta ggtcccatg ggaacaccg 
      ggacttccg ggaaaaacg ggacttgac ggtcctgca 
      ggacttcaa ggaccttcg ggacccccg ggtgaaaaa 
      ggcgacatc ggcgaaatc ggccctcca ggtcctcaa 
      ggaatgaca ggacctcaa ggcctacct ggccctcaa 
      ggacctccc ggattaaca ggagctcca ggcgttgct 
      ggggaaaat ggagctcca ggttctcca ggcttaaca 
      ggatcacct ggaaaaacc ggaagtccg ggtcctgca 
      ggacagaag ggagaccgg ggaaggaga ggtccgaaa 
      ggacaccga ggcgaaatg ggattatcg ggaattaaa 
      ggggatttg ggcgagaag gggaataaa ggggataag 
      ggacctcca ggtcctgaa ggacgtaaa ggagagaca 
      ggacctgaa ggtcccatc ggaattaaa ggaagtgat 
      ggtcctgcg ggacctcca ggaatggaa ggaccaccg 
      ggacctaaa ggagtggaa ggtcagacg ggaccaaag 
      ggcgaacct ggaccccca ggtcctgca ggaccacct 
      ggacctccg gcggaaatg ccgctactt ccaccggaa 
      atgctcttc gaacgaact tttagtcgg gaaaggcgc 
      aatgttgag gaaattatt tccgaatat gaagatatg 
      aacacggaa gcggaaaca gacgataat ggaaaattc 
      ctggaaatg tacaacaca atatattcc atgagacag 
      gacttagaa agggtgaaa aagcctata gggacccga 
      gaaaaccca gtccggtcc tgcaaagac ttgttttac 
      ggccaccct caattccct gatggatgg tactggatt 
      gaccccaac tcgggtatg accgacgac gccatctat 
      gtttattgc aacatgaca gctgagggc gaaacgtgc 
      gtttttccg gacgcccac agcgccaac atgcccaac 
      ataccctgg agacaagaa gggggcaaa aaagattgg 
      tactcccac ctacgcggc ggctttaaa ataacttat 
      gaaacagta ggcgtcgtc caaatgaca ttcctccgc 
      cttctgtac caagaagcc taccaaaat ttcaccttt 
      tcttgcata aacaccatc gcatggttt aacgaaaac 
      acgcaagac cacaaaatg gctcttaaa ttccgcggc 
      gacaacgag tacgaattt gcgcacaag aaaaccggt 
      ctgaaagtt atccaagat ggttgccaa gatggcacc 
      accaaaagt gagacaatt ttcgagtta cggaccaca 
      aaattggac cgtttgccc atcgttgac ttttttccg 
      cttgactat ggcgccccc aaccaagcg tttgggttc 
      acagtgggc cccatttgc ctcaagtag attctaacc 
      caagttttg tcatagcac cttatttat gaatttact 
      taggaaatg taaatattt tgcaacact gccaacttc 
      catatcgtg tgtacatac atttttaaa taaattttt 
      aaagctctg