Status
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Gene information
General information
TC031742
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002035,TC012989
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450989.1: 16199..38288
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031742
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03289

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larva partially dorsalnot closed - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen circumference irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031742 RA
      MSDDSESDD GTIKGPKEF IVEELSDSE DSSVAETSS 
      EDEQRERPK PEIPPEYWH IQKLVKYMK SGNQTATVI 
      SLCCLKDHD LTTEINQMA IQDIGGLEV LINLLETKD 
      LKCKLGSLS VLSELSQNE QIRRCITDL GGISLLVQN 
      LADPARDLQ ILVAETIYN VAQIRKARK HVRKFDGIP 
      KLVDLLDVN ENCLKTQKE QLTPDELEN VNIAKAAAK 
      ALWSVSRSR KNIQVMMRS GSVPLLAKL LRSVHMDVI 
      VPTVGTISQ CANEPSYQL AIQTEGMIK DIVQHLSAE 
      VEDATTRNL VRQAGGLDP LVNMARDPA TKEDKLLLA 
      AVTGAIWKT AISPENVER YDQLKTVEV LVKLLENAD 
      EDEMVLKNV VGALCECLK FKHNRDVLR RVNGIPYLV 
      NLLNYTFPP LLENVPMVL RECAEDESS MRIIEELDG 
      VRLIWSLLK NESPKVQAN AAWSLVPCI RYATDSGEM 
      VRCFVGGLE LIVNLLKSS DAHVLACVC AAIAEIAKD 
      IENLAVITD HGVVPMLVN LVHTQNVEL REHLASAIA 
      YCCAWGSNC KTFGRLGAI TPLVAYMAD SNAKVHRTT 
      ALALFHLSK NAFNCITMH ESGVVPFLL KAISTTDWD 
      LQEAAAGCL ANIRKLALE AETCHLIRN KDESSAEDD
    CDS sequence
    • >TC031742 RA
      atgtcggat gattctgaa tcagatgat ggtacaatc 
      aaaggacca aaagaattc atcgttgaa gaactttcc 
      gatagtgaa gattcatca gtagctgaa acatcatcc 
      gaagatgag caaagagaa cgcccaaaa cctgaaata 
      cctccagaa tattggcac attcaaaaa ttggttaaa 
      tacatgaaa tctggaaat caaactgcg actgttatt 
      tcgttatgt tgtttgaag gatcatgat ctcaccacg 
      gaaattaat caaatggcc atccaggat attggaggt 
      ttagaagtc ctgatcaac ctcctcgaa accaaagac 
      ttgaaatgc aaactcggt tctttgagc gttttgagc 
      gaactctcg caaaacgag caaatccgt cgctgtatc 
      accgaccta ggcggaatc tccctcctg gtccaaaac 
      cttgctgac ccagctcgt gatttacaa attctcgta 
      gctgagacc atctacaat gtggcccaa atacgcaaa 
      gcccgaaaa catgtacga aaatttgac ggtatccct 
      aaactagtc gatcttttg gacgttaac gaaaactgt 
      ctcaaaaca caaaaagaa caactcact ccagacgaa 
      ctcgaaaat gttaacatt gctaaagcg gcagctaaa 
      gccttatgg tcagtgtca cggagtagg aagaacatt 
      caagtgatg atgagaagt ggttcagtg ccacttttg 
      gcaaaacta ctcaggtcg gttcatatg gatgtgatt 
      gttccaact gttgggact atttcacag tgtgcgaat 
      gaaccatcg tatcagttg gctattcag actgaaggc 
      atgattaaa gatattgtg cagcatttg tcagcagag 
      gtggaagat gcaacaact cgtaatttg gtgcgacaa 
      gctggagga ttggaccct ttggtcaat atggcacga 
      gatccagcg accaaagaa gacaaattg ctccttgct 
      gcggtcact ggagccata tggaaaact gcaataagt 
      cccgaaaat gttgaacga tacgatcaa cttaaaacc 
      gttgaagtg ttggtaaaa ctattggaa aatgccgat 
      gaggacgaa atggttttg aaaaatgtg gttggtgcc 
      ctttgtgag tgtctcaaa tttaaacac aatcgtgat 
      gttttgaga cgtgttaat ggcattccg tatctcgtt 
      aatttgctc aattatacg tttcctcca cttttggaa 
      aatgttcca atggttttg agagaatgt gcggaagat 
      gaaagttcg atgaggatt atcgaagaa ttggatggg 
      gttagactg atttggtca cttttgaaa aacgagtct 
      cctaaggtc caagcaaac gctgcctgg tcccttgtc 
      ccttgtata cgctacgct actgattca ggcgaaatg 
      gttcgttgt ttcgtcggc ggactcgaa ctaatcgta 
      aacttgtta aaatcaagc gatgctcac gttttggct 
      tgtgtttgt gctgcaatc gctgaaata gccaaagat 
      atcgaaaat ttagctgtg ataactgat catggagtt 
      gtcccaatg ttggtaaac ctggtccac acacaaaat 
      gttgaattg agggaacat ttagcttca gctattgcc 
      tattgttgt gcatggggt tccaattgt aaaacgttc 
      ggtcgtttg ggtgccatc actcctctg gtggcctac 
      atggccgat tcgaacgca aaagtgcac cgaactaca 
      gctttggca ctctttcac ctgtcgaaa aatgccttc 
      aattgcatc acaatgcac gaaagtgga gttgttccc 
      tttctactc aaagccata tctaccaca gactgggac 
      ttgcaagag gcagcagcc ggctgtttg gccaatatc 
      aggaaattg gcactcgaa gcagaaact tgtcattta 
      atcagaaat aaagatgag tcgagtgca gaagacgac 
      taa
    mRNA sequence
    • >TC031742 RA
      ggccaacaa aaacgcaac ttaaaaagc atactagat 
      tctgaaaaa aatcaaatc ataaattta cgtacagtg 
      caacctcaa taatccgaa cccttggtg accccaaat 
      cacttaatc agcacaaaa tacaaatct ttttatttt 
      atgagcacc gataattaa taaattcga tggtccaaa 
      caccccttt ccttcaatt agttcggat taccggggt 
      tgtactgta gatattaat taaaccaga taaataatt 
      acgcaaaaa tttattcac taatgacag ctccaagtg 
      gaaagcttt gacaatttt atgacattt tgctaatca 
      aaaaacgct aaaaatgtc ggatgattc tgaatcaga 
      tgatggtac aatcaaagg accaaaaga attcatcgt 
      tgaagaact ttccgatag tgaagattc atcagtagc 
      tgaaacatc atccgaaga tgagcaaag agaacgccc 
      aaaacctga aatacctcc agaatattg gcacattca 
      aaaattggt taaatacat gaaatctgg aaatcaaac 
      tgcgactgt tatttcgtt atgttgttt gaaggatca 
      tgatctcac cacggaaat taatcaaat ggccatcca 
      ggatattgg aggtttaga agtcctgat caacctcct 
      cgaaaccaa agacttgaa atgcaaact cggttcttt 
      gagcgtttt gagcgaact ctcgcaaaa cgagcaaat 
      ccgtcgctg tatcaccga cctaggcgg aatctccct 
      cctggtcca aaaccttgc tgacccagc tcgtgattt 
      acaaattct cgtagctga gaccatcta caatgtggc 
      ccaaatacg caaagcccg aaaacatgt acgaaaatt 
      tgacggtat ccctaaact agtcgatct tttggacgt 
      taacgaaaa ctgtctcaa aacacaaaa agaacaact 
      cactccaga cgaactcga aaatgttaa cattgctaa 
      agcggcagc taaagcctt atggtcagt gtcacggag 
      taggaagaa cattcaagt gatgatgag aagtggttc 
      agtgccact tttggcaaa actactcag gtcggttca 
      tatggatgt gattgttcc aactgttgg gactatttc 
      acagtgtgc gaatgaacc atcgtatca gttggctat 
      tcagactga aggcatgat taaagatat tgtgcagca 
      tttgtcagc agaggtgga agatgcaac aactcgtaa 
      tttggtgcg acaagctgg aggattgga ccctttggt 
      caatatggc acgagatcc agcgaccaa agaagacaa 
      attgctcct tgctgcggt cactggagc catatggaa 
      aactgcaat aagtcccga aaatgttga acgatacga 
      tcaacttaa aaccgttga agtgttggt aaaactatt 
      ggaaaatgc cgatgagga cgaaatggt tttgaaaaa 
      tgtggttgg tgccctttg tgagtgtct caaatttaa 
      acacaatcg tgatgtttt gagacgtgt taatggcat 
      tccgtatct cgttaattt gctcaatta tacgtttcc 
      tccactttt ggaaaatgt tccaatggt tttgagaga 
      atgtgcgga agatgaaag ttcgatgag gattatcga 
      agaattgga tggggttag actgatttg gtcactttt 
      gaaaaacga gtctcctaa ggtccaagc aaacgctgc 
      ctggtccct tgtcccttg tatacgcta cgctactga 
      ttcaggcga aatggttcg ttgtttcgt cggcggact 
      cgaactaat cgtaaactt gttaaaatc aagcgatgc 
      tcacgtttt ggcttgtgt ttgtgctgc aatcgctga 
      aatagccaa agatatcga aaatttagc tgtgataac 
      tgatcatgg agttgtccc aatgttggt aaacctggt 
      ccacacaca aaatgttga attgaggga acatttagc 
      ttcagctat tgcctattg ttgtgcatg gggttccaa 
      ttgtaaaac gttcggtcg tttgggtgc catcactcc 
      tctggtggc ctacatggc cgattcgaa cgcaaaagt 
      gcaccgaac tacagcttt ggcactctt tcacctgtc 
      gaaaaatgc cttcaattg catcacaat gcacgaaag 
      tggagttgt tccctttct actcaaagc catatctac 
      cacagactg ggacttgca agaggcagc agccggctg 
      tttggccaa tatcaggaa attggcact cgaagcaga 
      aacttgtca tttaatcag aaataaaga tgagtcgag 
      tgcagaaga cgactaatt gaaggacaa agaatggcc 
      ttgttttgg tcaaagtac caactttga cgtattata 
      attaaaatt tacattttt attattaaa ataaatttt 
      taccttcat t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY