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Gene information
General information
TC031857 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015451805.1: 44943..53453
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031857
      iBeetle screen iB_03287

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031857 RA
        MTRPNLQNP KLITTDQTQ WCTNPYLNH 
        TKILTVSKI SIKTEINLT YLNHDKPN
      CDS sequence
      • >TC031857 RA
        atgacaaga ccaaattta caaaacccc 
        aaactaatc actacagac caaacccaa 
        tggtgtaca aacccttac ctcaaccac 
        accaaaatt ttaaccgtc agcaaaatt 
        tcaatcaaa accgaaatc aatctaacg 
        tatttaaac cacgacaaa cccaattag
      mRNA sequence
      • >TC031857 RA
        tgatacctt aaaggcttt agagcctct attcgactt 
        tcccagcga gtatcactt agtggtttt aaagtgagc 
        ccttcaaca tgttttaat aatatggcc caacggaaa 
        gttgacgaa gaaaaaaat aaataaatt ttttggacc 
        atattaaaa aaattagcg gcttctaag gatgccttt 
        gccatgtcg ttaacgact aaatttaag gaatggcta 
        ctacatggt acataaaat gctcgtgga tttaaaact 
        ctaattcta gattgtact cctgattta tcgcctttc 
        atattggct ccgttatca taggcttgt cctctcata 
        tcgtgcaaa gataatgct aattttgca agtttctca 
        attattaaa tttgaaagt tcttttcct gtcgatctt 
        tcaactgga agaaaccct aaaaagctt tcatttatt 
        ttaacggtt acttcatta gggttagat ttgtctaca 
        gacacatac cttactaaa aatgtcatt tgttcaacc 
        ttactcaca tcagaagta caatccaaa atgatcgaa 
        taatatttg gctttttta acttcgtta ataatatgt 
        aatttaata tttttatgt aaaagagaa attatttcg 
        ttctgaata ttttttcca agatagtgg gctttagtc 
        tcttctttt ttcagtact ctacgaaca tgttcttcc 
        attattggg tcaaactga gcaagtaac tgaattatt 
        tgtaaaaaa ttgccatta ttaaactaa tacaatttg 
        tcacttgta cctcgaaag gccaaagaa ttttgagca 
        agcattttg attcttgaa acgatgcgt agatggacc 
        tcgtaccaa taatttgtt tctgcagta ataagtctt 
        tgtgattca ctatcaatc gttttagaa cattgtaat 
        cgttggcta aattcaatc caagatttt tgatttaaa 
        atatgtttt tttgaggtt tcatgttct tttaaagtt 
        tcaataaga tgcttccaa cttttaatc ccttctgtt 
        gaaagtgaa ttaacagta caaccaaaa agtttgcag 
        caaaaacaa tataaacag ttttttgta atggaataa 
        actaaccag ttacgcatt actttttct ccatttttt 
        aaaattcta aaataataa cgtgtatta aaccttcga 
        cctgtgtca tcgtgtgga aagttatat tccgttaat 
        gccttgggt ggtccttgg tttactaaa aatgttctg 
        tcagcgtct gatagaata cttggccag gatgctgga 
        tcagaagtg atagtaagt gtctctccc ttggatgtc 
        cccgggata gcttctgtt tgaagagtg tcgatatcc 
        ataatattt tcgacggta gccccaaca tcaagatct 
        gcttctgtt tcattgtca gagaattct gggtcttcg 
        tcctttttt tttcgtact ttgtagaaa attcaataa 
        acttccagc cagtttttc actttggac ttttttttc 
        gagtcttct ctttttgta ttgagcacc agacaatct 
        ttttcgccc atcagacat tctaaaaat cattttaaa 
        ggagtatta gcacacgtt aaccatcga attgtgtaa 
        cttgcgaca aggcaacct gaaaccact agaattttt 
        cacaagaat tcaagaaaa cttgaatct gcaaatcag 
        ctatatttc gacccaagt agtcgttac tgccagaga 
        cgcattaat tcaaaattc gaatcattt ggccctaag 
        agtttttct aaaacattt aaacaaccc cttaggaga 
        ttatttatc cactagaca accggcaag tttgggcga 
        cgctttacg aattttgac caatgattt caacataaa 
        tgacaagac caaatttac aaaacccca aactaatca 
        ctacagacc aaacccaat ggtgtacaa acccttacc 
        tcaaccaca ccaaaattt taaccgtca gcaaaattt 
        caatcaaaa ccgaaatca atctaacgt atttaaacc 
        acgacaaac ccaattaga caaaccagt tccaatgag 
        tataagtac cagaaacac agttccaaa taaaccaaa 
        ttataattt tcgacaacc tcagcaaaa ttggtacgc 
        ggaagaatt gcacaacaa cgaaaatga agtaaatga 
        ccaatctga aattcaaga atgtcctga tgataataa 
        tgaaacaaa taacggtgc atcaacctc attttttga 
        accaaattt tgaacaata tacagaacc ttttttaga 
        agaagcagc ctcggacat gttgaatta ttaaatatt 
        gacgacaac ccttcacaa ttgccttat atcgttata 
        ccctttcca aactgtaaa ttaaaatgc ttaatcgac 
        actggttca acgaaatcg tttataaat ccagaaaaa 
        gcaaaactt cactttgct aaattaata aaaaatgac 
        ccgttta
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY