Status
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Gene information
General information
TC031748
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002005
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450989.1: 282244..320648
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031748
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03286

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Strong phenotypes do not show any head or thoracic segments.
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: Strong phenotypes do not show any head or thoracic segments.
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: Strong phenotypes do not show any head or thoracic segments.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031748 RA
      MADTELAKA LKDLPYRVQ TASLKQRKE VIESVIDVL 
      QNPAINESI IKGICRVIQ LTLPRYRDS SSQVLVRNL 
      IVALLEKHS DFAIKCLTL MLVDIATQH KNLVVTTNT 
      CQTGLYALH WSCLLVTVG WRVNENILQ THLSEIVEV 
      QAVFLTTVV AAGIEKRST KAFNLLNNS WNSIPESEK 
      LYLDKLSKS EQTQYVVVL AAAIAKRLT CINKNDLIA 
      PYINSLLET FIKNFVSCK TRPLPNVVI ATYPLLKQK 
      AMLRNPEVI LECVGLVIS GLNLDLSTC AVEIGNSLI 
      ANLYSKDDQ ARNEAADAC KRLAQQIKE PNAVTDLLK 
      KTFAVFHGS EGKLTVVDH KISVLQAIG YFSYNNVPE 
      DQLGEVLVN VSDCFLKIL ESEVHEKTL CFALEMFVL 
      WGCKFEKEV PAKVVDAFK NGMGLKTST PLVRISYIK 
      CMLTCFNRN TINQATCLV PTLLKSVDR AVAQPAQCL 
      PVTEGLCAS CMLLKIIAV SGEKETNFQ NLWNVILDM 
      DKQIFVSEK FLSTTGDDG LIHVMHLCE KLLIDHSKI 
      LNGKNSPLH RAVLYCVIF GSAKVRRKC LTILKRMVT 
      NSPKAALAR ALLRELLSF LETVKIQGE NNTEMSSHA 
      LVECITSLC SAQDLALED TNENALATT VSLNAEVIL 
      PSLIETIAT HLNDPRICQ VSKDDYFTF LTPEGELYD 
      KSVVPGNET NETMNLKRE SKVYSYKEQ LEELQLRRE 
      IEEKKKKDG KIKPPQYTP KQLEAIKNQ KMKEQAIRS 
      RLAMLNTSI INCVSMIRA AAKGNPLQL SLYFKDLLP 
      YILSGLQSP LAAPYLSKL YTDLRSTVF THQLETLGE 
      LIAFVTLRL LKPQCDIDA NWEAENLNK AMVRTVSLI 
      HEKTVAKNE TQNCFTVPA FCYTFVLLK LSLLSNYAR 
      NHDELVHDG LQIISEHAR LRATEQNGL KDLFDPQYL 
      PIKQMFELL VEIISSSSG RVQSQAEAC LLDVARCAS 
      GDPGCTKAT MDEIDVLLT GLQNPAVVV RDAALRSLT 
      IILLS
    CDS sequence
    • >TC031748 RA
      atggcagat acagagctt gcaaaagcc ctgaaggat 
      cttccatac cgcgtccaa accgcgagt ttaaaacag 
      cgaaaagag gttatagag agtgtgatt gatgttttg 
      caaaatccc gctatcaat gagagtatc atcaaggga 
      atttgtcgg gtgattcag ttaactttg ccccgatac 
      cgggacagt tcgtctcaa gttctggtg agaaattta 
      atcgtggca ttgcttgaa aaacattcc gattttgcc 
      ataaaatgc ctcactttg atgcttgtg gacattgcc 
      acccaacac aaaaatctg gtcgtgact acgaacaca 
      tgtcagaca gggttgtat gccctacac tggtcatgt 
      ctcttggtc acagttggt tggagagtc aacgaaaac 
      attctccaa acccactta tcggaaatc gtcgaagtt 
      caagccgtt tttttgaca acagttgtg gcagcaggg 
      attgaaaaa cgctcaact aaagcgttc aatctttta 
      aataattca tggaattca ataccagaa agtgaaaaa 
      ttgtattta gataaatta agcaagtcc gaacagacc 
      caatatgtg gttgtttta gcagcagcg atagctaaa 
      cgactgact tgtattaat aaaaatgat ttaatcgcc 
      ccgtacatt aattcactt ttggagact tttattaaa 
      aattttgtc agttgtaaa acgagacct ttgccaaat 
      gttgtaata gccacttat ccgttatta aaacaaaaa 
      gctatgttg cgaaacccc gaagtgatt ttagagtgt 
      gttggactt gtgatttca ggtcttaat ctagacttg 
      agcacttgt gctgttgaa attggaaac agtttaatt 
      gcaaatcta tactcaaag gacgaccaa gcaagaaac 
      gaagccgcc gatgcgtgc aaacgactc gctcaacaa 
      attaaagaa cccaatgct gttacagat ttattgaag 
      aagactttt gccgttttt cacggttct gagggaaaa 
      ttgactgtt gtcgatcat aaaataagt gttttgcaa 
      gccattggt tatttcagt tataacaat gtgcctgaa 
      gatcagttg ggggaagta ctggtcaat gtgtctgat 
      tgtttcttg aagattttg gagagtgag gttcatgag 
      aagacgctt tgtttcgct ttggagatg tttgttttg 
      tggggttgc aagtttgag aaggaggtg cctgccaaa 
      gttgtggac gcatttaag aatggcatg ggcttgaaa 
      acctctact cctctagtc cgtatatca tacataaaa 
      tgcatgctc acctgtttc aaccgaaac acaattaat 
      caagccacc tgtttagta ccaacatta ctaaaatca 
      gttgatcga gcagttgca caacctgca caatgtctc 
      cctgtaaca gaaggcttg tgtgcaagt tgcatgctc 
      ctgaaaata atcgcagta agtggtgaa aaagaaacc 
      aactttcaa aacctctgg aacgttatt ctcgacatg 
      gacaagcag attttcgtc tcggaaaaa ttcctatca 
      acaactggc gacgacggc ttgattcac gttatgcat 
      ctgtgcgaa aaactgcta attgatcat tcgaaaatc 
      ctaaatggg aaaaattca ccactacat cgtgcagtt 
      ctatactgt gtgattttt ggttcggcc aaagtgcgt 
      cgtaaatgt ttgacgatt ctcaagaga atggttaca 
      aattctccg aaggcagct ctggcaagg gctttgctc 
      agggagttg ttgagtttt ctcgaaact gttaaaatt 
      caaggagag aataatacg gaaatgtcc agtcatgca 
      ctagttgag tgtatcact tctttgtgt tcggcacag 
      gatttggca cttgaagat acgaatgaa aatgctttg 
      gcaacgact gtatcacta aacgccgaa gtaatttta 
      ccttcttta atcgaaaca attgccact catttgaat 
      gacccacga atatgtcaa gtatctaaa gacgattat 
      ttcactttt ttgactcct gaaggagaa ctttacgac 
      aaaagtgtt gttccagga aacgaaacg aatgaaaca 
      atgaatttg aaacgcgaa agtaaagtt tacagttat 
      aaagaacaa ttggaagaa ttgcaattg agacgcgaa 
      attgaagaa aagaaaaag aaagatgga aaaattaaa 
      ccaccacag tatacacct aaacaattg gaagccatc 
      aaaaatcaa aaaatgaaa gaacaagcg atacgatca 
      cgactagca atgttgaac acttctatc atcaattgt 
      gtttcgatg ataagagcc gctgcaaag ggcaatccc 
      ttgcaatta tctttatat ttcaaagac ttgcttccc 
      tacattttg agcggtttg caatcacca cttgccgct 
      ccttattta tccaaactt tacaccgac cttcgaagt 
      acagttttc acacaccag ttggaaact ttgggcgaa 
      cttattgcg ttcgttaca ttgagatta ttgaaacca 
      caatgtgat attgacgcg aattgggaa gccgagaat 
      ttaaacaaa gcaatggtc cgaactgta tcattgata 
      catgaaaaa accgttgcg aaaaatgag actcagaat 
      tgttttaca gttcctgcg ttttgttac acttttgtg 
      ttgttgaaa ttgagtctt ttatcgaat tatgcgaga 
      aatcacgac gagttggtt catgatggc ttacagatt 
      atttccgaa catgcaagg ttaagggcg acggaacaa 
      aatgggttg aaggatctt tttgatcca cagtatttg 
      cccatcaag cagatgttt gagttactg gtggaaatt 
      ataagtagt tcaagcggt cgagtgcaa tcgcaagcc 
      gaagcttgt cttctggat gtcgctcgt tgtgctagt 
      ggtgatcca ggttgtaca aaagcaaca atggacgaa 
      atcgacgtc cttttgacc ggtttacaa aacccagct 
      gttgtcgta agagatgca gctttacga agcttaaca 
      ataattctg ttgtcgtaa
    mRNA sequence
    • >TC031748 RA
      tgggaaaga tggcagata cagagcttg caaaagccc 
      tgaaggatc ttccatacc gcgtccaaa ccgcgagtt 
      taaaacagc gaaaagagg ttatagaga gtgtgattg 
      atgttttgc aaaatcccg ctatcaatg agagtatca 
      tcaagggaa tttgtcggg tgattcagt taactttgc 
      cccgatacc gggacagtt cgtctcaag ttctggtga 
      gaaatttaa tcgtggcat tgcttgaaa aacattccg 
      attttgcca taaaatgcc tcactttga tgcttgtgg 
      acattgcca cccaacaca aaaatctgg tcgtgacta 
      cgaacacat gtcagacag ggttgtatg ccctacact 
      ggtcatgtc tcttggtca cagttggtt ggagagtca 
      acgaaaaca ttctccaaa cccacttat cggaaatcg 
      tcgaagttc aagccgttt ttttgacaa cagttgtgg 
      cagcaggga ttgaaaaac gctcaacta aagcgttca 
      atcttttaa ataattcat ggaattcaa taccagaaa 
      gtgaaaaat tgtatttag ataaattaa gcaagtccg 
      aacagaccc aatatgtgg ttgttttag cagcagcga 
      tagctaaac gactgactt gtattaata aaaatgatt 
      taatcgccc cgtacatta attcacttt tggagactt 
      ttattaaaa attttgtca gttgtaaaa cgagacctt 
      tgccaaatg ttgtaatag ccacttatc cgttattaa 
      aacaaaaag ctatgttgc gaaaccccg aagtgattt 
      tagagtgtg ttggacttg tgatttcag gtcttaatc 
      tagacttga gcacttgtg ctgttgaaa ttggaaaca 
      gtttaattg caaatctat actcaaagg acgaccaag 
      caagaaacg aagccgccg atgcgtgca aacgactcg 
      ctcaacaaa ttaaagaac ccaatgctg ttacagatt 
      tattgaaga agacttttg ccgtttttc acggttctg 
      agggaaaat tgactgttg tcgatcata aaataagtg 
      ttttgcaag ccattggtt atttcagtt ataacaatg 
      tgcctgaag atcagttgg gggaagtac tggtcaatg 
      tgtctgatt gtttcttga agattttgg agagtgagg 
      ttcatgaga agacgcttt gtttcgctt tggagatgt 
      ttgttttgt ggggttgca agtttgaga aggaggtgc 
      ctgccaaag ttgtggacg catttaaga atggcatgg 
      gcttgaaaa cctctactc ctctagtcc gtatatcat 
      acataaaat gcatgctca cctgtttca accgaaaca 
      caattaatc aagccacct gtttagtac caacattac 
      taaaatcag ttgatcgag cagttgcac aacctgcac 
      aatgtctcc ctgtaacag aaggcttgt gtgcaagtt 
      gcatgctcc tgaaaataa tcgcagtaa gtggtgaaa 
      aagaaacca actttcaaa acctctgga acgttattc 
      tcgacatgg acaagcaga ttttcgtct cggaaaaat 
      tcctatcaa caactggcg acgacggct tgattcacg 
      ttatgcatc tgtgcgaaa aactgctaa ttgatcatt 
      cgaaaatcc taaatggga aaaattcac cactacatc 
      gtgcagttc tatactgtg tgatttttg gttcggcca 
      aagtgcgtc gtaaatgtt tgacgattc tcaagagaa 
      tggttacaa attctccga aggcagctc tggcaaggg 
      ctttgctca gggagttgt tgagttttc tcgaaactg 
      ttaaaattc aaggagaga ataatacgg aaatgtcca 
      gtcatgcac tagttgagt gtatcactt ctttgtgtt 
      cggcacagg atttggcac ttgaagata cgaatgaaa 
      atgctttgg caacgactg tatcactaa acgccgaag 
      taattttac cttctttaa tcgaaacaa ttgccactc 
      atttgaatg acccacgaa tatgtcaag tatctaaag 
      acgattatt tcacttttt tgactcctg aaggagaac 
      tttacgaca aaagtgttg ttccaggaa acgaaacga 
      atgaaacaa tgaatttga aacgcgaaa gtaaagttt 
      acagttata aagaacaat tggaagaat tgcaattga 
      gacgcgaaa ttgaagaaa agaaaaaga aagatggaa 
      aaattaaac caccacagt atacaccta aacaattgg 
      aagccatca aaaatcaaa aaatgaaag aacaagcga 
      tacgatcac gactagcaa tgttgaaca cttctatca 
      tcaattgtg tttcgatga taagagccg ctgcaaagg 
      gcaatccct tgcaattat ctttatatt tcaaagact 
      tgcttccct acattttga gcggtttgc aatcaccac 
      ttgccgctc cttatttat ccaaacttt acaccgacc 
      ttcgaagta cagttttca cacaccagt tggaaactt 
      tgggcgaac ttattgcgt tcgttacat tgagattat 
      tgaaaccac aatgtgata ttgacgcga attgggaag 
      ccgagaatt taaacaaag caatggtcc gaactgtat 
      cattgatac atgaaaaaa ccgttgcga aaaatgaga 
      ctcagaatt gttttacag ttcctgcgt tttgttaca 
      cttttgtgt tgttgaaat tgagtcttt tatcgaatt 
      atgcgagaa atcacgacg agttggttc atgatggct 
      tacagatta tttccgaac atgcaaggt taagggcga 
      cggaacaaa atgggttga aggatcttt ttgatccac 
      agtatttgc ccatcaagc agatgtttg agttactgg 
      tggaaatta taagtagtt caagcggtc gagtgcaat 
      cgcaagccg aagcttgtc ttctggatg tcgctcgtt 
      gtgctagtg gtgatccag gttgtacaa aagcaacaa 
      tggacgaaa tcgacgtcc ttttgaccg gtttacaaa 
      acccagctg ttgtcgtaa gagatgcag ctttacgaa 
      gcttaacaa taattctgt tgtcgtaag agatgcagc 
      tttacgaag cttaacaat aattgtctc ctcactacc 
      gaattacga aagcgatta tgaatacgc aatgaaaat 
      tagcaaaag aatatggat tgcgaaatt tgacgaaaa 
      tgaagataa ccaaatctt ggcagaaaa attatggga 
      attggcaaa attacaatt tccagcttg tctcagcga 
      agaattaat gcccgatat tgaacatcc agttgagtg 
      tgttcaagt tgcagcatc gagagctct tgctgctct 
      cttgcaaaa taatcaaga acaagtgca ggaaacttt 
      gcagaagct tttgaaatt gtacaatca acgattaca 
      ggttagtaa tttacataa g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY