Status
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Gene information
General information
TC031748
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC002005 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450989.1: 282244..320648
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031748
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03286

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
     embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    Comment: Strong phenotypes do not show any head or thoracic segments.
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    head shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: Strong phenotypes do not show any head or thoracic segments.
    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax position irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    thorax shape irregular - Penetrance (percentage of animals/eggs on slide): less than 30%
    abdomen not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Comment: Strong phenotypes do not show any head or thoracic segments.
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031748 RA
      MADTELAKA LKDLPYRVQ TASLKQRKE VIESVIDVL 
QNPAINESI IKGICRVIQ LTLPRYRDS SSQVLVRNL 
IVALLEKHS DFAIKCLTL MLVDIATQH KNLVVTTNT 
CQTGLYALH WSCLLVTVG WRVNENILQ THLSEIVEV 
QAVFLTTVV AAGIEKRST KAFNLLNNS WNSIPESEK 
LYLDKLSKS EQTQYVVVL AAAIAKRLT CINKNDLIA 
PYINSLLET FIKNFVSCK TRPLPNVVI ATYPLLKQK 
AMLRNPEVI LECVGLVIS GLNLDLSTC AVEIGNSLI 
ANLYSKDDQ ARNEAADAC KRLAQQIKE PNAVTDLLK 
KTFAVFHGS EGKLTVVDH KISVLQAIG YFSYNNVPE 
DQLGEVLVN VSDCFLKIL ESEVHEKTL CFALEMFVL 
WGCKFEKEV PAKVVDAFK NGMGLKTST PLVRISYIK 
CMLTCFNRN TINQATCLV PTLLKSVDR AVAQPAQCL 
PVTEGLCAS CMLLKIIAV SGEKETNFQ NLWNVILDM 
DKQIFVSEK FLSTTGDDG LIHVMHLCE KLLIDHSKI 
LNGKNSPLH RAVLYCVIF GSAKVRRKC LTILKRMVT 
NSPKAALAR ALLRELLSF LETVKIQGE NNTEMSSHA 
LVECITSLC SAQDLALED TNENALATT VSLNAEVIL 
PSLIETIAT HLNDPRICQ VSKDDYFTF LTPEGELYD 
KSVVPGNET NETMNLKRE SKVYSYKEQ LEELQLRRE 
IEEKKKKDG KIKPPQYTP KQLEAIKNQ KMKEQAIRS 
RLAMLNTSI INCVSMIRA AAKGNPLQL SLYFKDLLP 
YILSGLQSP LAAPYLSKL YTDLRSTVF THQLETLGE 
LIAFVTLRL LKPQCDIDA NWEAENLNK AMVRTVSLI 
HEKTVAKNE TQNCFTVPA FCYTFVLLK LSLLSNYAR 
NHDELVHDG LQIISEHAR LRATEQNGL KDLFDPQYL 
PIKQMFELL VEIISSSSG RVQSQAEAC LLDVARCAS 
GDPGCTKAT MDEIDVLLT GLQNPAVVV RDAALRSLT 
IILLS
    CDS sequence
    • >TC031748 RA
      atggcagat acagagctt gcaaaagcc ctgaaggat 
cttccatac cgcgtccaa accgcgagt ttaaaacag 
cgaaaagag gttatagag agtgtgatt gatgttttg 
caaaatccc gctatcaat gagagtatc atcaaggga 
atttgtcgg gtgattcag ttaactttg ccccgatac 
cgggacagt tcgtctcaa gttctggtg agaaattta 
atcgtggca ttgcttgaa aaacattcc gattttgcc 
ataaaatgc ctcactttg atgcttgtg gacattgcc 
acccaacac aaaaatctg gtcgtgact acgaacaca 
tgtcagaca gggttgtat gccctacac tggtcatgt 
ctcttggtc acagttggt tggagagtc aacgaaaac 
attctccaa acccactta tcggaaatc gtcgaagtt 
caagccgtt tttttgaca acagttgtg gcagcaggg 
attgaaaaa cgctcaact aaagcgttc aatctttta 
aataattca tggaattca ataccagaa agtgaaaaa 
ttgtattta gataaatta agcaagtcc gaacagacc 
caatatgtg gttgtttta gcagcagcg atagctaaa 
cgactgact tgtattaat aaaaatgat ttaatcgcc 
ccgtacatt aattcactt ttggagact tttattaaa 
aattttgtc agttgtaaa acgagacct ttgccaaat 
gttgtaata gccacttat ccgttatta aaacaaaaa 
gctatgttg cgaaacccc gaagtgatt ttagagtgt 
gttggactt gtgatttca ggtcttaat ctagacttg 
agcacttgt gctgttgaa attggaaac agtttaatt 
gcaaatcta tactcaaag gacgaccaa gcaagaaac 
gaagccgcc gatgcgtgc aaacgactc gctcaacaa 
attaaagaa cccaatgct gttacagat ttattgaag 
aagactttt gccgttttt cacggttct gagggaaaa 
ttgactgtt gtcgatcat aaaataagt gttttgcaa 
gccattggt tatttcagt tataacaat gtgcctgaa 
gatcagttg ggggaagta ctggtcaat gtgtctgat 
tgtttcttg aagattttg gagagtgag gttcatgag 
aagacgctt tgtttcgct ttggagatg tttgttttg 
tggggttgc aagtttgag aaggaggtg cctgccaaa 
gttgtggac gcatttaag aatggcatg ggcttgaaa 
acctctact cctctagtc cgtatatca tacataaaa 
tgcatgctc acctgtttc aaccgaaac acaattaat 
caagccacc tgtttagta ccaacatta ctaaaatca 
gttgatcga gcagttgca caacctgca caatgtctc 
cctgtaaca gaaggcttg tgtgcaagt tgcatgctc 
ctgaaaata atcgcagta agtggtgaa aaagaaacc 
aactttcaa aacctctgg aacgttatt ctcgacatg 
gacaagcag attttcgtc tcggaaaaa ttcctatca 
acaactggc gacgacggc ttgattcac gttatgcat 
ctgtgcgaa aaactgcta attgatcat tcgaaaatc 
ctaaatggg aaaaattca ccactacat cgtgcagtt 
ctatactgt gtgattttt ggttcggcc aaagtgcgt 
cgtaaatgt ttgacgatt ctcaagaga atggttaca 
aattctccg aaggcagct ctggcaagg gctttgctc 
agggagttg ttgagtttt ctcgaaact gttaaaatt 
caaggagag aataatacg gaaatgtcc agtcatgca 
ctagttgag tgtatcact tctttgtgt tcggcacag 
gatttggca cttgaagat acgaatgaa aatgctttg 
gcaacgact gtatcacta aacgccgaa gtaatttta 
ccttcttta atcgaaaca attgccact catttgaat 
gacccacga atatgtcaa gtatctaaa gacgattat 
ttcactttt ttgactcct gaaggagaa ctttacgac 
aaaagtgtt gttccagga aacgaaacg aatgaaaca 
atgaatttg aaacgcgaa agtaaagtt tacagttat 
aaagaacaa ttggaagaa ttgcaattg agacgcgaa 
attgaagaa aagaaaaag aaagatgga aaaattaaa 
ccaccacag tatacacct aaacaattg gaagccatc 
aaaaatcaa aaaatgaaa gaacaagcg atacgatca 
cgactagca atgttgaac acttctatc atcaattgt 
gtttcgatg ataagagcc gctgcaaag ggcaatccc 
ttgcaatta tctttatat ttcaaagac ttgcttccc 
tacattttg agcggtttg caatcacca cttgccgct 
ccttattta tccaaactt tacaccgac cttcgaagt 
acagttttc acacaccag ttggaaact ttgggcgaa 
cttattgcg ttcgttaca ttgagatta ttgaaacca 
caatgtgat attgacgcg aattgggaa gccgagaat 
ttaaacaaa gcaatggtc cgaactgta tcattgata 
catgaaaaa accgttgcg aaaaatgag actcagaat 
tgttttaca gttcctgcg ttttgttac acttttgtg 
ttgttgaaa ttgagtctt ttatcgaat tatgcgaga 
aatcacgac gagttggtt catgatggc ttacagatt 
atttccgaa catgcaagg ttaagggcg acggaacaa 
aatgggttg aaggatctt tttgatcca cagtatttg 
cccatcaag cagatgttt gagttactg gtggaaatt 
ataagtagt tcaagcggt cgagtgcaa tcgcaagcc 
gaagcttgt cttctggat gtcgctcgt tgtgctagt 
ggtgatcca ggttgtaca aaagcaaca atggacgaa 
atcgacgtc cttttgacc ggtttacaa aacccagct 
gttgtcgta agagatgca gctttacga agcttaaca 
ataattctg ttgtcgtaa
    mRNA sequence
    • >TC031748 RA
      tgggaaaga tggcagata cagagcttg caaaagccc 
tgaaggatc ttccatacc gcgtccaaa ccgcgagtt 
taaaacagc gaaaagagg ttatagaga gtgtgattg 
atgttttgc aaaatcccg ctatcaatg agagtatca 
tcaagggaa tttgtcggg tgattcagt taactttgc 
cccgatacc gggacagtt cgtctcaag ttctggtga 
gaaatttaa tcgtggcat tgcttgaaa aacattccg 
attttgcca taaaatgcc tcactttga tgcttgtgg 
acattgcca cccaacaca aaaatctgg tcgtgacta 
cgaacacat gtcagacag ggttgtatg ccctacact 
ggtcatgtc tcttggtca cagttggtt ggagagtca 
acgaaaaca ttctccaaa cccacttat cggaaatcg 
tcgaagttc aagccgttt ttttgacaa cagttgtgg 
cagcaggga ttgaaaaac gctcaacta aagcgttca 
atcttttaa ataattcat ggaattcaa taccagaaa 
gtgaaaaat tgtatttag ataaattaa gcaagtccg 
aacagaccc aatatgtgg ttgttttag cagcagcga 
tagctaaac gactgactt gtattaata aaaatgatt 
taatcgccc cgtacatta attcacttt tggagactt 
ttattaaaa attttgtca gttgtaaaa cgagacctt 
tgccaaatg ttgtaatag ccacttatc cgttattaa 
aacaaaaag ctatgttgc gaaaccccg aagtgattt 
tagagtgtg ttggacttg tgatttcag gtcttaatc 
tagacttga gcacttgtg ctgttgaaa ttggaaaca 
gtttaattg caaatctat actcaaagg acgaccaag 
caagaaacg aagccgccg atgcgtgca aacgactcg 
ctcaacaaa ttaaagaac ccaatgctg ttacagatt 
tattgaaga agacttttg ccgtttttc acggttctg 
agggaaaat tgactgttg tcgatcata aaataagtg 
ttttgcaag ccattggtt atttcagtt ataacaatg 
tgcctgaag atcagttgg gggaagtac tggtcaatg 
tgtctgatt gtttcttga agattttgg agagtgagg 
ttcatgaga agacgcttt gtttcgctt tggagatgt 
ttgttttgt ggggttgca agtttgaga aggaggtgc 
ctgccaaag ttgtggacg catttaaga atggcatgg 
gcttgaaaa cctctactc ctctagtcc gtatatcat 
acataaaat gcatgctca cctgtttca accgaaaca 
caattaatc aagccacct gtttagtac caacattac 
taaaatcag ttgatcgag cagttgcac aacctgcac 
aatgtctcc ctgtaacag aaggcttgt gtgcaagtt 
gcatgctcc tgaaaataa tcgcagtaa gtggtgaaa 
aagaaacca actttcaaa acctctgga acgttattc 
tcgacatgg acaagcaga ttttcgtct cggaaaaat 
tcctatcaa caactggcg acgacggct tgattcacg 
ttatgcatc tgtgcgaaa aactgctaa ttgatcatt 
cgaaaatcc taaatggga aaaattcac cactacatc 
gtgcagttc tatactgtg tgatttttg gttcggcca 
aagtgcgtc gtaaatgtt tgacgattc tcaagagaa 
tggttacaa attctccga aggcagctc tggcaaggg 
ctttgctca gggagttgt tgagttttc tcgaaactg 
ttaaaattc aaggagaga ataatacgg aaatgtcca 
gtcatgcac tagttgagt gtatcactt ctttgtgtt 
cggcacagg atttggcac ttgaagata cgaatgaaa 
atgctttgg caacgactg tatcactaa acgccgaag 
taattttac cttctttaa tcgaaacaa ttgccactc 
atttgaatg acccacgaa tatgtcaag tatctaaag 
acgattatt tcacttttt tgactcctg aaggagaac 
tttacgaca aaagtgttg ttccaggaa acgaaacga 
atgaaacaa tgaatttga aacgcgaaa gtaaagttt 
acagttata aagaacaat tggaagaat tgcaattga 
gacgcgaaa ttgaagaaa agaaaaaga aagatggaa 
aaattaaac caccacagt atacaccta aacaattgg 
aagccatca aaaatcaaa aaatgaaag aacaagcga 
tacgatcac gactagcaa tgttgaaca cttctatca 
tcaattgtg tttcgatga taagagccg ctgcaaagg 
gcaatccct tgcaattat ctttatatt tcaaagact 
tgcttccct acattttga gcggtttgc aatcaccac 
ttgccgctc cttatttat ccaaacttt acaccgacc 
ttcgaagta cagttttca cacaccagt tggaaactt 
tgggcgaac ttattgcgt tcgttacat tgagattat 
tgaaaccac aatgtgata ttgacgcga attgggaag 
ccgagaatt taaacaaag caatggtcc gaactgtat 
cattgatac atgaaaaaa ccgttgcga aaaatgaga 
ctcagaatt gttttacag ttcctgcgt tttgttaca 
cttttgtgt tgttgaaat tgagtcttt tatcgaatt 
atgcgagaa atcacgacg agttggttc atgatggct 
tacagatta tttccgaac atgcaaggt taagggcga 
cggaacaaa atgggttga aggatcttt ttgatccac 
agtatttgc ccatcaagc agatgtttg agttactgg 
tggaaatta taagtagtt caagcggtc gagtgcaat 
cgcaagccg aagcttgtc ttctggatg tcgctcgtt 
gtgctagtg gtgatccag gttgtacaa aagcaacaa 
tggacgaaa tcgacgtcc ttttgaccg gtttacaaa 
acccagctg ttgtcgtaa gagatgcag ctttacgaa 
gcttaacaa taattctgt tgtcgtaag agatgcagc 
tttacgaag cttaacaat aattgtctc ctcactacc 
gaattacga aagcgatta tgaatacgc aatgaaaat 
tagcaaaag aatatggat tgcgaaatt tgacgaaaa 
tgaagataa ccaaatctt ggcagaaaa attatggga 
attggcaaa attacaatt tccagcttg tctcagcga 
agaattaat gcccgatat tgaacatcc agttgagtg 
tgttcaagt tgcagcatc gagagctct tgctgctct 
cttgcaaaa taatcaaga acaagtgca ggaaacttt 
gcagaagct tttgaaatt gtacaatca acgattaca 
ggttagtaa tttacataa g
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY