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Gene information
General information
TC002002 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450989.1: 158163..166979
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC002002
    iBeetle screen iB_03285

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC002002 RA
      MGIHEGLAK EITKIGNII RSNWSRESL VEHKKQLEL 
      NLSTQVWIE ETKPQFAQT YAEEVAFDG IPDVFPKEE 
      NTEQDPLEI IGQEEASTS ETFDKNKTL LSLSKFLKT 
      KNNSKKVTI EDLQQFCKE KKCKVIVCK NDTRELSAQ 
      LKMQQQIIE TMDKKIEQI SKKVDQLEQ QQNTTQGPF 
      RLDISRTFD SPTSEACTI LLPSRKPEP ANQLIKPPT 
      RPILSARKV GKDVVLKWR MPYCSSKVY APVASYEVY 
      CYILNGSKR WRRMSKVES SELSMTFTL KNIDDDLIY 
      YFGVRAIDV YKRRGLFST EVTINNSKI SSGCS
    CDS sequence
    • >TC002002 RA
      atgggaata catgaagga ttggcaaaa gaaatcact 
      aaaattgga aacattatt cgttctaac tggagtaga 
      gaatcgctg gttgagcat aaaaaacaa cttgaattg 
      aacctctcg acacaagtg tggattgaa gaaaccaaa 
      ccacaattt gcacaaact tacgcagaa gaagttgct 
      tttgatgga atcccagac gtatttccc aaagaagaa 
      aacactgag caagatccc ctcgaaatc ataggacaa 
      gaagaagcg agcacaagt gaaaccttc gacaaaaac 
      aaaactctc ttatcgcta tcaaaattt ttaaaaacg 
      aaaaacaat agtaaaaaa gtaacaatt gaagacttg 
      cagcagttt tgcaaagag aagaaatgt aaagtaatt 
      gtgtgtaaa aatgataca agggagctt agcgcacag 
      ttaaaaatg caacaacaa ataatcgag acaatggat 
      aagaaaatt gaacaaatc tcgaaaaaa gtcgatcag 
      ttagagcaa caacaaaat acaacacaa ggaccattc 
      cgtttagat atttctcga acgttcgat tcaccaaca 
      agtgaagct tgtactatt ttattaccc tctaggaag 
      ccagaacct gcaaatcaa cttatcaaa cctccaaca 
      cgacctata ctaagtgct agaaaagtg ggcaaagat 
      gttgttctt aaatggaga atgccttac tgttctagt 
      aaggtttac gcacctgtt gcaagttat gaagtctat 
      tgttatata ttgaatggt agtaaaaga tggcggagg 
      atgtctaaa gtggaatcg tctgaactt tctatgact 
      tttacttta aagaatatt gatgatgac ttgatttat 
      tattttggt gttcgtgcg attgatgtt tataaaaga 
      aggggtttg tttagtaca gaagtgact attaataat 
      tccaagata tcttccggt tgtagctag
    mRNA sequence
    • >TC002002 RA
      agagttgtc aaactgtta ttatttata ttatatgta 
      acaatgggt cggagaaat aaaaaggca aatgtgaaa 
      aaattatgg tgggaccgt aaaaggtac aaaatgcct 
      catcttata acgaactat agcaccgga caatgggaa 
      tacatgaag gattggcaa aagaaatca ctaaaattg 
      gaaacatta ttcgttcta actggagta gagaatcgc 
      tggttgagc ataaaaaac aacttgaat tgaacctct 
      cgacacaag tgtggattg aagaaacca aaccacaat 
      ttgcacaaa cttacgcag aagaagttg cttttgatg 
      gaatcccag acgtatttc ccaaagaag aaaacactg 
      agcaagatc ccctcgaaa tcataggac aagaagaag 
      cgagcacaa gtgaaacct tcgacaaaa acaaaactc 
      tcttatcgc tatcaaaat ttttaaaaa cgaaaaaca 
      atagtaaaa aagtaacaa ttgaagact tgcagcagt 
      tttgcaaag agaagaaat gtaaagtaa ttgtgtgta 
      aaaatgata caagggagc ttagcgcac agttaaaaa 
      tgcaacaac aaataatcg agacaatgg ataagaaaa 
      ttgaacaaa tctcgaaaa aagtcgatc agttagagc 
      aacaacaaa atacaacac aaggaccat tccgtttag 
      atatttctc gaacgttcg attcaccaa caagtgaag 
      cttgtacta ttttattac cctctagga agccagaac 
      ctgcaaatc aacttatca aacctccaa cacgaccta 
      tactaagtg ctagaaaag tgggcaaag atgttgttc 
      ttaaatgga gaatgcctt actgttcta gtaaggttt 
      acgcacctg ttgcaagtt atgaagtct attgttata 
      tattgaatg gtagtaaaa gatggcgga ggatgtcta 
      aagtggaat cgtctgaac tttctatga cttttactt 
      taaagaata ttgatgatg acttgattt attattttg 
      gtgttcgtg cgattgatg tttataaaa gaaggggtt 
      tgtttagta cagaagtga ctattaata attccaaga 
      tatcttccg gttgtagct agggttttt tctattaaa 
      taaaagtta aaaaattat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY