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Gene information
General information
TC001997 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid861: 247115..247953
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC001997
      iBeetle screen iB_03284
      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 100.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
      Days post injection


      adult lethality - Number of animals showing the phenotype: 5 (11 dpi)
      Days post injection


      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001997 RA
        MAASPSFNE NELAMCYYY GFTSSQLVE 
        DFREKTQDV IKTNITSLE EIINLKFPG 
        QQEAIKEQT TNLLRLFLE IAETPLRQF 
        EIKSREIFA LPQNASAFT EVNIEPVES 
        GEIEKLEKE VSELMKEYR EEACYKSYL 
        EKQIERYEQ VEPLRQEID EILKFHEKN 
        KVKPGEKTI KELHNAMKT LELQVYEKQ 
        LIK
      CDS sequence
      • >TC001997 RA
        atggcagcc agtcctagt tttaacgaa aacgaactt 
        gcgatgtgt tattactac ggttttact tcgtctcaa 
        ttagtggaa gattttcgt gaaaaaacc caggatgtg 
        attaaaacc aacataact tcccttgaa gagattata 
        aatttaaaa tttcctggc cagcaggag gcgattaag 
        gagcagaca accaatctg ttgaggctt tttcttgaa 
        attgccgaa actccttta agacaattt gaaattaaa 
        tcacgggag attttcgca cttccacag aatgctagc 
        gctttcacc gaagtgaac attgaacct gttgagagt 
        ggagagatt gaaaaattg gagaaggag gtttctgag 
        ttaatgaag gagtacaga gaagaggct tgttataaa 
        agttatttg gagaaacag atagagcgg tatgagcaa 
        gttgaacct ttacgtcag gaaatcgat gagatattg 
        aaatttcac gaaaaaaat aaagtaaaa ccgggcgag 
        aaaactatc aaagagtta cacaacgcg atgaagact 
        ttggagctc caggtttac gagaagcaa ttaataaag 
        tga
      mRNA sequence
      • >TC001997 RA
        cgtagttca caacgagct tagtgacaa acacaaact 
        aaaaatccc tagattcaa atttgtgcg acgagtttg 
        ttggttaaa aataatctt actttttta attatgtgt 
        cttgtttaa atacgaaat ggcagccag tcctagttt 
        taacgaaaa cgaacttgc gatgtgtta ttactacgg 
        ttttacttc gtctcaatt agtggaaga ttttcgtga 
        aaaaaccca ggatgtgat taaaaccaa cataacttc 
        ccttgaaga gattataaa tttaaaatt tcctggcca 
        gcaggaggc gattaagga gcagacaac caatctgtt 
        gaggctttt tcttgaaat tgccgaaac tcctttaag 
        acaatttga aattaaatc acgggagat tttcgcact 
        tccacagaa tgctagcgc tttcaccga agtgaacat 
        tgaacctgt tgagagtgg agagattga aaaattgga 
        gaaggaggt ttctgagtt aatgaagga gtacagaga 
        agaggcttg ttataaaag ttatttgga gaaacagat 
        agagcggta tgagcaagt tgaaccttt acgtcagga 
        aatcgatga gatattgaa atttcacga aaaaaataa 
        agtaaaacc gggcgagaa aactatcaa agagttaca 
        caacgcgat gaagacttt ggagctcca ggtttacga 
        gaagcaatt aataaagtg aggtggact gtacttttt 
        tataaacaa atgcaaaaa tatttaagt aattgtcta 
        tttctactt ttcattaaa ataaaatta aaaaattaa 
        a