Status
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Gene information
General information
TC031834
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001981,TC001980
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450289.1: 122975..160863
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031834
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03283

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031834 RA
      MLPRICDVS VACSLVLCC LIGASLANS EFNDQDAVA 
      CSIRQFACA NKKCIPISY VCDGDDDCE DGSDEGVKE 
      CPSNNREHT EHQEPEYST STLKFLTTL VVCTSIIVT 
      MDLILKIAI VALLNVYAV TALVEENKT CEKNEFRCS 
      SGRCIPAHW QCDHEQDCN DGSDEDEHV CQQKVCGVD 
      EFTCRSASG ECVPLTWMC DDNPDCSDG SDEKACNET 
      CRSDEFTCK NGKCIQKRW VCDLDNDCG DNSDEKDCP 
      PVTCAPDSE FQCAENFCI TSKWRCDGE YDCVDGKDE 
      QGCPKMATS SSFCLSEEY ECGDHLTCI HKGWLCDGT 
      RDCPDGSDE SPDNCQNIT CRADQFQCK DRSCIAGHL 
      HCSGHPECP DGSDELNCG SPAPKCDPK TEFDCGGGN 
      CIPLSQVCD KRQHCPDGE DEPVGRCGI NECNENNGG 
      CSQRCVDTP SSFYCDCNH GYKLVDNRT CKDINECEI 
      PGSCSQICI NDKGGFKCD CQSGYMRDP RDLTKCKAI 
      EGHASLLFA RRRDIRKIS LDHHEMTSI VNETNSATA 
      LDFVFRTGM IFWSDVTDK KIYKAPIDE GNERSVVIS 
      NDITTSDGL AVDWIYNHI YWTDTGKNT IELANFDGK 
      MRKVLIKDE LEEPRALAL NPLEGWMFW TDWGSDPKI 
      ERSGMDGSH RQAIVTYDV KWPNGLTLD LVKKRVYWV 
      DAKLNVISS CDYNGRNRR LILFSPDSL RHPFSITTF 
      EDWVYWTDW DKAAVFKAN KFTGSDVQP VTATAMIQN 
      PMVIHVYHP YRQPDGENH CQAVNGHCS HLCLPAPQI 
      NAKSPRISC ACPENLRML KDGLTCVQD ETYTTTVNS 
      ITYTDYSSM SDYMTESTT NKSHTTNVV PESSDDGAV 
      AVIVIGISF AVIVIIGVV VYFLYRKYV KRNMTSMNF 
      DNPVYRKTT EDQFSLEKN QYPPSRPYL STVGEEAQQ 
      PLTGANNQN DNV
    CDS sequence
    • >TC031834 RA
      atgctgccg cgaatatgc gatgtttcc gttgcatgc 
      agcttggtt ttgtgttgt ctaatcggt gcttctttg 
      gccaattcg gaatttaac gaccaggat gctgttgcc 
      tgttcgata aggcagttt gcttgtgcc aacaaaaaa 
      tgcatcccg atatcgtac gtctgcgac ggtgacgac 
      gattgtgaa gacggatca gacgaaggc gtcaaagaa 
      tgcccaagt aataatagg gagcataca gaacatcaa 
      gaaccggag tactccaca tccacactc aaattcctg 
      acaactctc gtggtgtgc acatcaatt attgtgaca 
      atggattta atcctcaaa attgccatc gtggccctc 
      ctcaacgtc tacgccgtc acggccctg gtggaagag 
      aataaaacg tgtgaaaaa aatgagttc cgatgcagc 
      agtggtcgc tgtataccg gcccattgg caatgtgat 
      cacgaacaa gactgtaac gacggcagc gacgaagat 
      gagcatgtt tgtcaacaa aaagtgtgc ggcgttgac 
      gagttcaca tgtcgctcg gcgtcaggt gaatgcgtt 
      cccctaaca tggatgtgc gatgacaac cccgactgt 
      tccgacggc agtgacgag aaagcgtgc aatgagact 
      tgtagatcg gatgagttc acttgtaaa aacgggaaa 
      tgtatacag aaaagatgg gtttgtgat ttagataac 
      gactgcgga gataactcg gatgagaag gattgtccg 
      cctgtcact tgtgccccc gattcggag tttcaatgc 
      gctgaaaac ttttgtatc acgagcaag tggaggtgc 
      gacggcgag tatgactgc gtcgatgga aaagatgaa 
      caaggatgc ccgaaaatg gcgactagc agttccttc 
      tgcctctcc gaagagtac gagtgcgga gatcattta 
      acttgcatc cacaaaggc tggttgtgt gatggtaca 
      agggactgc cccgatggc tccgacgag tctcccgac 
      aattgtcaa aacatcact tgtagagcc gatcagttc 
      cagtgtaag gatcgctcg tgtattgca ggacattta 
      cattgttcg ggacatccg gaatgtcca gacggcagc 
      gacgaactc aattgtggc tctccggcg ccgaagtgc 
      gacccgaag acggagttc gattgcggc ggaggtaat 
      tgcatacct ctctcccaa gtttgcgat aagcgtcag 
      cattgtcct gacggcgag gacgagcca gttggccga 
      tgtgggatt aatgaatgc aatgaaaat aatgggggt 
      tgtagccag cgttgtgtc gacactcca tcgtcgttt 
      tactgcgac tgtaaccat ggctacaaa ctggttgat 
      aacaggacg tgtaaagat atcaatgag tgcgagatt 
      ccgggcagt tgttcgcag atttgtatc aatgataaa 
      gggggcttc aagtgcgat tgccagagc gggtacatg 
      cgcgaccct cgcgacctt accaagtgc aaagcaatc 
      gaagggcac gcctccctg ctcttcgcc cggaggagg 
      gacatccgg aagatttct ttggatcat cacgaaatg 
      accagtatt gtgaatgag acgaattcg gccacagcg 
      ctcgatttt gtctttcgg actggaatg atcttctgg 
      agcgacgtc acggacaaa aagatatac aaagctccg 
      attgatgaa ggaaatgag aggtcggtc gtgatcagt 
      aatgacata acgacttca gacggcctc gcggttgac 
      tggatctac aaccacatc tattggaca gataccggg 
      aagaacaca atagaactg gcgaatttc gatgggaaa 
      atgcgcaaa gttttaatc aaagacgaa ttagaagaa 
      cccagagcg ctcgcactt aaccctttg gaaggctgg 
      atgttctgg acggattgg ggaagtgac ccaaaaatt 
      gaaagatcg ggcatggac ggatcacac agacaggct 
      attgtaaca tatgacgtt aaatggccc aatggattg 
      actctggat ttagtcaaa aaacgggtc tattgggtc 
      gatgctaag ctaaatgtg atttcctcc tgtgattat 
      aatggaagg aacaggaga cttatactc ttctcgccg 
      gattctctc agacatcca ttttcgatc actaccttc 
      gaagattgg gtctattgg accgactgg gataaagcc 
      gccgttttc aaagccaac aaattcacc ggaagcgac 
      gtacaaccc gttacagcc actgctatg atccagaac 
      ccgatggtc atccacgtc taccaccct taccgccag 
      cccgatggg gagaaccat tgccaggcg gtgaacggg 
      cattgttcg cacctgtgc ttgcctgcg ccccaaata 
      aacgcgaaa tctccgcgt atttcctgt gcatgtccc 
      gagaactta aggatgttg aaagatgga ttgacttgc 
      gttcaagac gaaacatat acgaccacc gtaaattct 
      ataacttat acagattat tcatcaatg tcagattat 
      atgactgaa agtacaact aacaagtcg cacacaact 
      aacgtagtc cctgaaagt tccgatgac ggtgctgtt 
      gctgttatt gtaattgga atatctttt gccgtgata 
      gtcataatc ggtgttgtg gtgtacttc ctgtataga 
      aaatatgtg aaacgtaac atgacgtct atgaatttc 
      gataatcct gtttatcgt aaaacaacc gaagatcaa 
      ttttcctta gagaaaaac caatatccg ccatcccga 
      ccgtattta agtacagtt ggtgaagag gctcaacag 
      cctctaaca ggtgccaac aaccaaaat gacaatgtt 
      taa
    mRNA sequence
    • >TC031834 RA
      ctcaaagca cgtcagtct tcgacgaag gtgggtctt 
      gtgcacggc agacggtcg tctgcatag aaataagca 
      tagtcggtg cagagaaaa attggttat gtcgtaaag 
      cacgtgcga gcccaccct taagaaaat gctgccgcg 
      aatatgcga tgtttccgt tgcatgcag cttggtttt 
      gtgttgtct aatcggtgc ttctttggc caattcgga 
      atttaacga ccaggatgc tgttgcctg ttcgataag 
      gcagtttgc ttgtgccaa caaaaaatg catcccgat 
      atcgtacgt ctgcgacgg tgacgacga ttgtgaaga 
      cggatcaga cgaaggcgt caaagaatg cccaagtaa 
      taataggga gcatacaga acatcaaga accggagta 
      ctccacatc cacactcaa attcctgac aactctcgt 
      ggtgtgcac atcaattat tgtgacaat ggatttaat 
      cctcaaaat tgccatcgt ggccctcct caacgtcta 
      cgccgtcac ggccctggt ggaagagaa taaaacgtg 
      tgaaaaaaa tgagttccg atgcagcag tggtcgctg 
      tataccggc ccattggca atgtgatca cgaacaaga 
      ctgtaacga cggcagcga cgaagatga gcatgtttg 
      tcaacaaaa agtgtgcgg cgttgacga gttcacatg 
      tcgctcggc gtcaggtga atgcgttcc cctaacatg 
      gatgtgcga tgacaaccc cgactgttc cgacggcag 
      tgacgagaa agcgtgcaa tgagacttg tagatcgga 
      tgagttcac ttgtaaaaa cgggaaatg tatacagaa 
      aagatgggt ttgtgattt agataacga ctgcggaga 
      taactcgga tgagaagga ttgtccgcc tgtcacttg 
      tgcccccga ttcggagtt tcaatgcgc tgaaaactt 
      ttgtatcac gagcaagtg gaggtgcga cggcgagta 
      tgactgcgt cgatggaaa agatgaaca aggatgccc 
      gaaaatggc gactagcag ttccttctg cctctccga 
      agagtacga gtgcggaga tcatttaac ttgcatcca 
      caaaggctg gttgtgtga tggtacaag ggactgccc 
      cgatggctc cgacgagtc tcccgacaa ttgtcaaaa 
      catcacttg tagagccga tcagttcca gtgtaagga 
      tcgctcgtg tattgcagg acatttaca ttgttcggg 
      acatccgga atgtccaga cggcagcga cgaactcaa 
      ttgtggctc tccggcgcc gaagtgcga cccgaagac 
      ggagttcga ttgcggcgg aggtaattg catacctct 
      ctcccaagt ttgcgataa gcgtcagca ttgtcctga 
      cggcgagga cgagccagt tggccgatg tgggattaa 
      tgaatgcaa tgaaaataa tgggggttg tagccagcg 
      ttgtgtcga cactccatc gtcgtttta ctgcgactg 
      taaccatgg ctacaaact ggttgataa caggacgtg 
      taaagatat caatgagtg cgagattcc gggcagttg 
      ttcgcagat ttgtatcaa tgataaagg gggcttcaa 
      gtgcgattg ccagagcgg gtacatgcg cgaccctcg 
      cgaccttac caagtgcaa agcaatcga agggcacgc 
      ctccctgct cttcgcccg gaggaggga catccggaa 
      gatttcttt ggatcatca cgaaatgac cagtattgt 
      gaatgagac gaattcggc cacagcgct cgattttgt 
      ctttcggac tggaatgat cttctggag cgacgtcac 
      ggacaaaaa gatatacaa agctccgat tgatgaagg 
      aaatgagag gtcggtcgt gatcagtaa tgacataac 
      gacttcaga cggcctcgc ggttgactg gatctacaa 
      ccacatcta ttggacaga taccgggaa gaacacaat 
      agaactggc gaatttcga tgggaaaat gcgcaaagt 
      tttaatcaa agacgaatt agaagaacc cagagcgct 
      cgcacttaa ccctttgga aggctggat gttctggac 
      ggattgggg aagtgaccc aaaaattga aagatcggg 
      catggacgg atcacacag acaggctat tgtaacata 
      tgacgttaa atggcccaa tggattgac tctggattt 
      agtcaaaaa acgggtcta ttgggtcga tgctaagct 
      aaatgtgat ttcctcctg tgattataa tggaaggaa 
      caggagact tatactctt ctcgccgga ttctctcag 
      acatccatt ttcgatcac taccttcga agattgggt 
      ctattggac cgactggga taaagccgc cgttttcaa 
      agccaacaa attcaccgg aagcgacgt acaacccgt 
      tacagccac tgctatgat ccagaaccc gatggtcat 
      ccacgtcta ccaccctta ccgccagcc cgatgggga 
      gaaccattg ccaggcggt gaacgggca ttgttcgca 
      cctgtgctt gcctgcgcc ccaaataaa cgcgaaatc 
      tccgcgtat ttcctgtgc atgtcccga gaacttaag 
      gatgttgaa agatggatt gacttgcgt tcaagacga 
      aacatatac gaccaccgt aaattctat aacttatac 
      agattattc atcaatgtc agattatat gactgaaag 
      tacaactaa caagtcgca cacaactaa cgtagtccc 
      tgaaagttc cgatgacgg tgctgttgc tgttattgt 
      aattggaat atcttttgc cgtgatagt cataatcgg 
      tgttgtggt gtacttcct gtatagaaa atatgtgaa 
      acgtaacat gacgtctat gaatttcga taatcctgt 
      ttatcgtaa aacaaccga agatcaatt ttccttaga 
      gaaaaacca atatccgcc atcccgacc gtatttaag 
      tacagttgg tgaagaggc tcaacagcc tctaacagg 
      tgccaacaa ccaaaatga caatgttta aacagtgaa 
      attttttta ctcaaagtg ctcaattac agtgaaatt 
      aaagactta gatttaatt tgacaaagt catcatttc 
      tttttgtat agatgtgta tttattgta ccatcgtaa 
      aaagaacta actattagt gtgttgtta aaattccag 
      tttcgtgtc aaaccacag actgaatta agttcattg 
      cgaaagtcc agaggaatt tgtcaaaat ttttcgtaa 
      actgatttc ctaatttac tatttgtga tctcagtgg 
      aaaatggga ttttaacga tttggatga aagtgagtg 
      agattcgag aggtatggt taatttgtc caaagagtc 
      gtattagca attaatcta agccaattg taattttta 
      atagtcacg ttttctcca atgttaagt ttatttttc 
      gaattcgta cctcgatat ttttaattt atctatttt 
      cattgctca tgtttgtat atattaaat tttttagaa 
      atgtttgta ttgttaaat tatttatta cactacgat 
      gttaagctt gtaactatt ttactgcga gaaatcaaa 
      atgtaaaaa gttgtatat ttcagtcat aaacatata 
      ctaatgtag ctctaaaat tctgtgatt atattgttt 
      gtattcctt ttcaatttt ctaggaatt tacattttt 
      actgtctag attatttat cacatccta actttttgt 
      tttatttgt gtaattatt aaaatttaa ataaatata 
      tgtacaaga t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY