| General information | |||
| TC031834
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450289.1: 122975..160863 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
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| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC031834 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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| Publish name | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03283 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
ovary without detectable defects - Number of animals showing the phenotype: 1
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031834 RA
MLPRICDVS VACSLVLCC LIGASLANS EFNDQDAVA CSIRQFACA NKKCIPISY VCDGDDDCE DGSDEGVKE CPSNNREHT EHQEPEYST STLKFLTTL VVCTSIIVT MDLILKIAI VALLNVYAV TALVEENKT CEKNEFRCS SGRCIPAHW QCDHEQDCN DGSDEDEHV CQQKVCGVD EFTCRSASG ECVPLTWMC DDNPDCSDG SDEKACNET CRSDEFTCK NGKCIQKRW VCDLDNDCG DNSDEKDCP PVTCAPDSE FQCAENFCI TSKWRCDGE YDCVDGKDE QGCPKMATS SSFCLSEEY ECGDHLTCI HKGWLCDGT RDCPDGSDE SPDNCQNIT CRADQFQCK DRSCIAGHL HCSGHPECP DGSDELNCG SPAPKCDPK TEFDCGGGN CIPLSQVCD KRQHCPDGE DEPVGRCGI NECNENNGG CSQRCVDTP SSFYCDCNH GYKLVDNRT CKDINECEI PGSCSQICI NDKGGFKCD CQSGYMRDP RDLTKCKAI EGHASLLFA RRRDIRKIS LDHHEMTSI VNETNSATA LDFVFRTGM IFWSDVTDK KIYKAPIDE GNERSVVIS NDITTSDGL AVDWIYNHI YWTDTGKNT IELANFDGK MRKVLIKDE LEEPRALAL NPLEGWMFW TDWGSDPKI ERSGMDGSH RQAIVTYDV KWPNGLTLD LVKKRVYWV DAKLNVISS CDYNGRNRR LILFSPDSL RHPFSITTF EDWVYWTDW DKAAVFKAN KFTGSDVQP VTATAMIQN PMVIHVYHP YRQPDGENH CQAVNGHCS HLCLPAPQI NAKSPRISC ACPENLRML KDGLTCVQD ETYTTTVNS ITYTDYSSM SDYMTESTT NKSHTTNVV PESSDDGAV AVIVIGISF AVIVIIGVV VYFLYRKYV KRNMTSMNF DNPVYRKTT EDQFSLEKN QYPPSRPYL STVGEEAQQ PLTGANNQN DNV
- >TC031834 RA
atgctgccg cgaatatgc gatgtttcc gttgcatgc agcttggtt ttgtgttgt ctaatcggt gcttctttg gccaattcg gaatttaac gaccaggat gctgttgcc tgttcgata aggcagttt gcttgtgcc aacaaaaaa tgcatcccg atatcgtac gtctgcgac ggtgacgac gattgtgaa gacggatca gacgaaggc gtcaaagaa tgcccaagt aataatagg gagcataca gaacatcaa gaaccggag tactccaca tccacactc aaattcctg acaactctc gtggtgtgc acatcaatt attgtgaca atggattta atcctcaaa attgccatc gtggccctc ctcaacgtc tacgccgtc acggccctg gtggaagag aataaaacg tgtgaaaaa aatgagttc cgatgcagc agtggtcgc tgtataccg gcccattgg caatgtgat cacgaacaa gactgtaac gacggcagc gacgaagat gagcatgtt tgtcaacaa aaagtgtgc ggcgttgac gagttcaca tgtcgctcg gcgtcaggt gaatgcgtt cccctaaca tggatgtgc gatgacaac cccgactgt tccgacggc agtgacgag aaagcgtgc aatgagact tgtagatcg gatgagttc acttgtaaa aacgggaaa tgtatacag aaaagatgg gtttgtgat ttagataac gactgcgga gataactcg gatgagaag gattgtccg cctgtcact tgtgccccc gattcggag tttcaatgc gctgaaaac ttttgtatc acgagcaag tggaggtgc gacggcgag tatgactgc gtcgatgga aaagatgaa caaggatgc ccgaaaatg gcgactagc agttccttc tgcctctcc gaagagtac gagtgcgga gatcattta acttgcatc cacaaaggc tggttgtgt gatggtaca agggactgc cccgatggc tccgacgag tctcccgac aattgtcaa aacatcact tgtagagcc gatcagttc cagtgtaag gatcgctcg tgtattgca ggacattta cattgttcg ggacatccg gaatgtcca gacggcagc gacgaactc aattgtggc tctccggcg ccgaagtgc gacccgaag acggagttc gattgcggc ggaggtaat tgcatacct ctctcccaa gtttgcgat aagcgtcag cattgtcct gacggcgag gacgagcca gttggccga tgtgggatt aatgaatgc aatgaaaat aatgggggt tgtagccag cgttgtgtc gacactcca tcgtcgttt tactgcgac tgtaaccat ggctacaaa ctggttgat aacaggacg tgtaaagat atcaatgag tgcgagatt ccgggcagt tgttcgcag atttgtatc aatgataaa gggggcttc aagtgcgat tgccagagc gggtacatg cgcgaccct cgcgacctt accaagtgc aaagcaatc gaagggcac gcctccctg ctcttcgcc cggaggagg gacatccgg aagatttct ttggatcat cacgaaatg accagtatt gtgaatgag acgaattcg gccacagcg ctcgatttt gtctttcgg actggaatg atcttctgg agcgacgtc acggacaaa aagatatac aaagctccg attgatgaa ggaaatgag aggtcggtc gtgatcagt aatgacata acgacttca gacggcctc gcggttgac tggatctac aaccacatc tattggaca gataccggg aagaacaca atagaactg gcgaatttc gatgggaaa atgcgcaaa gttttaatc aaagacgaa ttagaagaa cccagagcg ctcgcactt aaccctttg gaaggctgg atgttctgg acggattgg ggaagtgac ccaaaaatt gaaagatcg ggcatggac ggatcacac agacaggct attgtaaca tatgacgtt aaatggccc aatggattg actctggat ttagtcaaa aaacgggtc tattgggtc gatgctaag ctaaatgtg atttcctcc tgtgattat aatggaagg aacaggaga cttatactc ttctcgccg gattctctc agacatcca ttttcgatc actaccttc gaagattgg gtctattgg accgactgg gataaagcc gccgttttc aaagccaac aaattcacc ggaagcgac gtacaaccc gttacagcc actgctatg atccagaac ccgatggtc atccacgtc taccaccct taccgccag cccgatggg gagaaccat tgccaggcg gtgaacggg cattgttcg cacctgtgc ttgcctgcg ccccaaata aacgcgaaa tctccgcgt atttcctgt gcatgtccc gagaactta aggatgttg aaagatgga ttgacttgc gttcaagac gaaacatat acgaccacc gtaaattct ataacttat acagattat tcatcaatg tcagattat atgactgaa agtacaact aacaagtcg cacacaact aacgtagtc cctgaaagt tccgatgac ggtgctgtt gctgttatt gtaattgga atatctttt gccgtgata gtcataatc ggtgttgtg gtgtacttc ctgtataga aaatatgtg aaacgtaac atgacgtct atgaatttc gataatcct gtttatcgt aaaacaacc gaagatcaa ttttcctta gagaaaaac caatatccg ccatcccga ccgtattta agtacagtt ggtgaagag gctcaacag cctctaaca ggtgccaac aaccaaaat gacaatgtt taa
- >TC031834 RA
ctcaaagca cgtcagtct tcgacgaag gtgggtctt gtgcacggc agacggtcg tctgcatag aaataagca tagtcggtg cagagaaaa attggttat gtcgtaaag cacgtgcga gcccaccct taagaaaat gctgccgcg aatatgcga tgtttccgt tgcatgcag cttggtttt gtgttgtct aatcggtgc ttctttggc caattcgga atttaacga ccaggatgc tgttgcctg ttcgataag gcagtttgc ttgtgccaa caaaaaatg catcccgat atcgtacgt ctgcgacgg tgacgacga ttgtgaaga cggatcaga cgaaggcgt caaagaatg cccaagtaa taataggga gcatacaga acatcaaga accggagta ctccacatc cacactcaa attcctgac aactctcgt ggtgtgcac atcaattat tgtgacaat ggatttaat cctcaaaat tgccatcgt ggccctcct caacgtcta cgccgtcac ggccctggt ggaagagaa taaaacgtg tgaaaaaaa tgagttccg atgcagcag tggtcgctg tataccggc ccattggca atgtgatca cgaacaaga ctgtaacga cggcagcga cgaagatga gcatgtttg tcaacaaaa agtgtgcgg cgttgacga gttcacatg tcgctcggc gtcaggtga atgcgttcc cctaacatg gatgtgcga tgacaaccc cgactgttc cgacggcag tgacgagaa agcgtgcaa tgagacttg tagatcgga tgagttcac ttgtaaaaa cgggaaatg tatacagaa aagatgggt ttgtgattt agataacga ctgcggaga taactcgga tgagaagga ttgtccgcc tgtcacttg tgcccccga ttcggagtt tcaatgcgc tgaaaactt ttgtatcac gagcaagtg gaggtgcga cggcgagta tgactgcgt cgatggaaa agatgaaca aggatgccc gaaaatggc gactagcag ttccttctg cctctccga agagtacga gtgcggaga tcatttaac ttgcatcca caaaggctg gttgtgtga tggtacaag ggactgccc cgatggctc cgacgagtc tcccgacaa ttgtcaaaa catcacttg tagagccga tcagttcca gtgtaagga tcgctcgtg tattgcagg acatttaca ttgttcggg acatccgga atgtccaga cggcagcga cgaactcaa ttgtggctc tccggcgcc gaagtgcga cccgaagac ggagttcga ttgcggcgg aggtaattg catacctct ctcccaagt ttgcgataa gcgtcagca ttgtcctga cggcgagga cgagccagt tggccgatg tgggattaa tgaatgcaa tgaaaataa tgggggttg tagccagcg ttgtgtcga cactccatc gtcgtttta ctgcgactg taaccatgg ctacaaact ggttgataa caggacgtg taaagatat caatgagtg cgagattcc gggcagttg ttcgcagat ttgtatcaa tgataaagg gggcttcaa gtgcgattg ccagagcgg gtacatgcg cgaccctcg cgaccttac caagtgcaa agcaatcga agggcacgc ctccctgct cttcgcccg gaggaggga catccggaa gatttcttt ggatcatca cgaaatgac cagtattgt gaatgagac gaattcggc cacagcgct cgattttgt ctttcggac tggaatgat cttctggag cgacgtcac ggacaaaaa gatatacaa agctccgat tgatgaagg aaatgagag gtcggtcgt gatcagtaa tgacataac gacttcaga cggcctcgc ggttgactg gatctacaa ccacatcta ttggacaga taccgggaa gaacacaat agaactggc gaatttcga tgggaaaat gcgcaaagt tttaatcaa agacgaatt agaagaacc cagagcgct cgcacttaa ccctttgga aggctggat gttctggac ggattgggg aagtgaccc aaaaattga aagatcggg catggacgg atcacacag acaggctat tgtaacata tgacgttaa atggcccaa tggattgac tctggattt agtcaaaaa acgggtcta ttgggtcga tgctaagct aaatgtgat ttcctcctg tgattataa tggaaggaa caggagact tatactctt ctcgccgga ttctctcag acatccatt ttcgatcac taccttcga agattgggt ctattggac cgactggga taaagccgc cgttttcaa agccaacaa attcaccgg aagcgacgt acaacccgt tacagccac tgctatgat ccagaaccc gatggtcat ccacgtcta ccaccctta ccgccagcc cgatgggga gaaccattg ccaggcggt gaacgggca ttgttcgca cctgtgctt gcctgcgcc ccaaataaa cgcgaaatc tccgcgtat ttcctgtgc atgtcccga gaacttaag gatgttgaa agatggatt gacttgcgt tcaagacga aacatatac gaccaccgt aaattctat aacttatac agattattc atcaatgtc agattatat gactgaaag tacaactaa caagtcgca cacaactaa cgtagtccc tgaaagttc cgatgacgg tgctgttgc tgttattgt aattggaat atcttttgc cgtgatagt cataatcgg tgttgtggt gtacttcct gtatagaaa atatgtgaa acgtaacat gacgtctat gaatttcga taatcctgt ttatcgtaa aacaaccga agatcaatt ttccttaga gaaaaacca atatccgcc atcccgacc gtatttaag tacagttgg tgaagaggc tcaacagcc tctaacagg tgccaacaa ccaaaatga caatgttta aacagtgaa attttttta ctcaaagtg ctcaattac agtgaaatt aaagactta gatttaatt tgacaaagt catcatttc tttttgtat agatgtgta tttattgta ccatcgtaa aaagaacta actattagt gtgttgtta aaattccag tttcgtgtc aaaccacag actgaatta agttcattg cgaaagtcc agaggaatt tgtcaaaat ttttcgtaa actgatttc ctaatttac tatttgtga tctcagtgg aaaatggga ttttaacga tttggatga aagtgagtg agattcgag aggtatggt taatttgtc caaagagtc gtattagca attaatcta agccaattg taattttta atagtcacg ttttctcca atgttaagt ttatttttc gaattcgta cctcgatat ttttaattt atctatttt cattgctca tgtttgtat atattaaat tttttagaa atgtttgta ttgttaaat tatttatta cactacgat gttaagctt gtaactatt ttactgcga gaaatcaaa atgtaaaaa gttgtatat ttcagtcat aaacatata ctaatgtag ctctaaaat tctgtgatt atattgttt gtattcctt ttcaatttt ctaggaatt tacattttt actgtctag attatttat cacatccta actttttgt tttatttgt gtaattatt aaaatttaa ataaatata tgtacaaga t
