Status
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Gene information
General information
TC031834
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001981,TC001980 		Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450289.1: 122975..160863
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC031834
    iBeetle screen iB_03283

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Fertility
    mature egg deposition blocked number 1st lateral oviduct: 2 number 2nd lateral oviduct: 3 - Number of animals showing the phenotype: 3
    ovary without detectable defects - Number of animals showing the phenotype: 1
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031834 RA
      MLPRICDVS VACSLVLCC LIGASLANS EFNDQDAVA 
CSIRQFACA NKKCIPISY VCDGDDDCE DGSDEGVKE 
CPSNNREHT EHQEPEYST STLKFLTTL VVCTSIIVT 
MDLILKIAI VALLNVYAV TALVEENKT CEKNEFRCS 
SGRCIPAHW QCDHEQDCN DGSDEDEHV CQQKVCGVD 
EFTCRSASG ECVPLTWMC DDNPDCSDG SDEKACNET 
CRSDEFTCK NGKCIQKRW VCDLDNDCG DNSDEKDCP 
PVTCAPDSE FQCAENFCI TSKWRCDGE YDCVDGKDE 
QGCPKMATS SSFCLSEEY ECGDHLTCI HKGWLCDGT 
RDCPDGSDE SPDNCQNIT CRADQFQCK DRSCIAGHL 
HCSGHPECP DGSDELNCG SPAPKCDPK TEFDCGGGN 
CIPLSQVCD KRQHCPDGE DEPVGRCGI NECNENNGG 
CSQRCVDTP SSFYCDCNH GYKLVDNRT CKDINECEI 
PGSCSQICI NDKGGFKCD CQSGYMRDP RDLTKCKAI 
EGHASLLFA RRRDIRKIS LDHHEMTSI VNETNSATA 
LDFVFRTGM IFWSDVTDK KIYKAPIDE GNERSVVIS 
NDITTSDGL AVDWIYNHI YWTDTGKNT IELANFDGK 
MRKVLIKDE LEEPRALAL NPLEGWMFW TDWGSDPKI 
ERSGMDGSH RQAIVTYDV KWPNGLTLD LVKKRVYWV 
DAKLNVISS CDYNGRNRR LILFSPDSL RHPFSITTF 
EDWVYWTDW DKAAVFKAN KFTGSDVQP VTATAMIQN 
PMVIHVYHP YRQPDGENH CQAVNGHCS HLCLPAPQI 
NAKSPRISC ACPENLRML KDGLTCVQD ETYTTTVNS 
ITYTDYSSM SDYMTESTT NKSHTTNVV PESSDDGAV 
AVIVIGISF AVIVIIGVV VYFLYRKYV KRNMTSMNF 
DNPVYRKTT EDQFSLEKN QYPPSRPYL STVGEEAQQ 
PLTGANNQN DNV
    CDS sequence
    • >TC031834 RA
      atgctgccg cgaatatgc gatgtttcc gttgcatgc 
agcttggtt ttgtgttgt ctaatcggt gcttctttg 
gccaattcg gaatttaac gaccaggat gctgttgcc 
tgttcgata aggcagttt gcttgtgcc aacaaaaaa 
tgcatcccg atatcgtac gtctgcgac ggtgacgac 
gattgtgaa gacggatca gacgaaggc gtcaaagaa 
tgcccaagt aataatagg gagcataca gaacatcaa 
gaaccggag tactccaca tccacactc aaattcctg 
acaactctc gtggtgtgc acatcaatt attgtgaca 
atggattta atcctcaaa attgccatc gtggccctc 
ctcaacgtc tacgccgtc acggccctg gtggaagag 
aataaaacg tgtgaaaaa aatgagttc cgatgcagc 
agtggtcgc tgtataccg gcccattgg caatgtgat 
cacgaacaa gactgtaac gacggcagc gacgaagat 
gagcatgtt tgtcaacaa aaagtgtgc ggcgttgac 
gagttcaca tgtcgctcg gcgtcaggt gaatgcgtt 
cccctaaca tggatgtgc gatgacaac cccgactgt 
tccgacggc agtgacgag aaagcgtgc aatgagact 
tgtagatcg gatgagttc acttgtaaa aacgggaaa 
tgtatacag aaaagatgg gtttgtgat ttagataac 
gactgcgga gataactcg gatgagaag gattgtccg 
cctgtcact tgtgccccc gattcggag tttcaatgc 
gctgaaaac ttttgtatc acgagcaag tggaggtgc 
gacggcgag tatgactgc gtcgatgga aaagatgaa 
caaggatgc ccgaaaatg gcgactagc agttccttc 
tgcctctcc gaagagtac gagtgcgga gatcattta 
acttgcatc cacaaaggc tggttgtgt gatggtaca 
agggactgc cccgatggc tccgacgag tctcccgac 
aattgtcaa aacatcact tgtagagcc gatcagttc 
cagtgtaag gatcgctcg tgtattgca ggacattta 
cattgttcg ggacatccg gaatgtcca gacggcagc 
gacgaactc aattgtggc tctccggcg ccgaagtgc 
gacccgaag acggagttc gattgcggc ggaggtaat 
tgcatacct ctctcccaa gtttgcgat aagcgtcag 
cattgtcct gacggcgag gacgagcca gttggccga 
tgtgggatt aatgaatgc aatgaaaat aatgggggt 
tgtagccag cgttgtgtc gacactcca tcgtcgttt 
tactgcgac tgtaaccat ggctacaaa ctggttgat 
aacaggacg tgtaaagat atcaatgag tgcgagatt 
ccgggcagt tgttcgcag atttgtatc aatgataaa 
gggggcttc aagtgcgat tgccagagc gggtacatg 
cgcgaccct cgcgacctt accaagtgc aaagcaatc 
gaagggcac gcctccctg ctcttcgcc cggaggagg 
gacatccgg aagatttct ttggatcat cacgaaatg 
accagtatt gtgaatgag acgaattcg gccacagcg 
ctcgatttt gtctttcgg actggaatg atcttctgg 
agcgacgtc acggacaaa aagatatac aaagctccg 
attgatgaa ggaaatgag aggtcggtc gtgatcagt 
aatgacata acgacttca gacggcctc gcggttgac 
tggatctac aaccacatc tattggaca gataccggg 
aagaacaca atagaactg gcgaatttc gatgggaaa 
atgcgcaaa gttttaatc aaagacgaa ttagaagaa 
cccagagcg ctcgcactt aaccctttg gaaggctgg 
atgttctgg acggattgg ggaagtgac ccaaaaatt 
gaaagatcg ggcatggac ggatcacac agacaggct 
attgtaaca tatgacgtt aaatggccc aatggattg 
actctggat ttagtcaaa aaacgggtc tattgggtc 
gatgctaag ctaaatgtg atttcctcc tgtgattat 
aatggaagg aacaggaga cttatactc ttctcgccg 
gattctctc agacatcca ttttcgatc actaccttc 
gaagattgg gtctattgg accgactgg gataaagcc 
gccgttttc aaagccaac aaattcacc ggaagcgac 
gtacaaccc gttacagcc actgctatg atccagaac 
ccgatggtc atccacgtc taccaccct taccgccag 
cccgatggg gagaaccat tgccaggcg gtgaacggg 
cattgttcg cacctgtgc ttgcctgcg ccccaaata 
aacgcgaaa tctccgcgt atttcctgt gcatgtccc 
gagaactta aggatgttg aaagatgga ttgacttgc 
gttcaagac gaaacatat acgaccacc gtaaattct 
ataacttat acagattat tcatcaatg tcagattat 
atgactgaa agtacaact aacaagtcg cacacaact 
aacgtagtc cctgaaagt tccgatgac ggtgctgtt 
gctgttatt gtaattgga atatctttt gccgtgata 
gtcataatc ggtgttgtg gtgtacttc ctgtataga 
aaatatgtg aaacgtaac atgacgtct atgaatttc 
gataatcct gtttatcgt aaaacaacc gaagatcaa 
ttttcctta gagaaaaac caatatccg ccatcccga 
ccgtattta agtacagtt ggtgaagag gctcaacag 
cctctaaca ggtgccaac aaccaaaat gacaatgtt 
taa
    mRNA sequence
    • >TC031834 RA
      ctcaaagca cgtcagtct tcgacgaag gtgggtctt 
gtgcacggc agacggtcg tctgcatag aaataagca 
tagtcggtg cagagaaaa attggttat gtcgtaaag 
cacgtgcga gcccaccct taagaaaat gctgccgcg 
aatatgcga tgtttccgt tgcatgcag cttggtttt 
gtgttgtct aatcggtgc ttctttggc caattcgga 
atttaacga ccaggatgc tgttgcctg ttcgataag 
gcagtttgc ttgtgccaa caaaaaatg catcccgat 
atcgtacgt ctgcgacgg tgacgacga ttgtgaaga 
cggatcaga cgaaggcgt caaagaatg cccaagtaa 
taataggga gcatacaga acatcaaga accggagta 
ctccacatc cacactcaa attcctgac aactctcgt 
ggtgtgcac atcaattat tgtgacaat ggatttaat 
cctcaaaat tgccatcgt ggccctcct caacgtcta 
cgccgtcac ggccctggt ggaagagaa taaaacgtg 
tgaaaaaaa tgagttccg atgcagcag tggtcgctg 
tataccggc ccattggca atgtgatca cgaacaaga 
ctgtaacga cggcagcga cgaagatga gcatgtttg 
tcaacaaaa agtgtgcgg cgttgacga gttcacatg 
tcgctcggc gtcaggtga atgcgttcc cctaacatg 
gatgtgcga tgacaaccc cgactgttc cgacggcag 
tgacgagaa agcgtgcaa tgagacttg tagatcgga 
tgagttcac ttgtaaaaa cgggaaatg tatacagaa 
aagatgggt ttgtgattt agataacga ctgcggaga 
taactcgga tgagaagga ttgtccgcc tgtcacttg 
tgcccccga ttcggagtt tcaatgcgc tgaaaactt 
ttgtatcac gagcaagtg gaggtgcga cggcgagta 
tgactgcgt cgatggaaa agatgaaca aggatgccc 
gaaaatggc gactagcag ttccttctg cctctccga 
agagtacga gtgcggaga tcatttaac ttgcatcca 
caaaggctg gttgtgtga tggtacaag ggactgccc 
cgatggctc cgacgagtc tcccgacaa ttgtcaaaa 
catcacttg tagagccga tcagttcca gtgtaagga 
tcgctcgtg tattgcagg acatttaca ttgttcggg 
acatccgga atgtccaga cggcagcga cgaactcaa 
ttgtggctc tccggcgcc gaagtgcga cccgaagac 
ggagttcga ttgcggcgg aggtaattg catacctct 
ctcccaagt ttgcgataa gcgtcagca ttgtcctga 
cggcgagga cgagccagt tggccgatg tgggattaa 
tgaatgcaa tgaaaataa tgggggttg tagccagcg 
ttgtgtcga cactccatc gtcgtttta ctgcgactg 
taaccatgg ctacaaact ggttgataa caggacgtg 
taaagatat caatgagtg cgagattcc gggcagttg 
ttcgcagat ttgtatcaa tgataaagg gggcttcaa 
gtgcgattg ccagagcgg gtacatgcg cgaccctcg 
cgaccttac caagtgcaa agcaatcga agggcacgc 
ctccctgct cttcgcccg gaggaggga catccggaa 
gatttcttt ggatcatca cgaaatgac cagtattgt 
gaatgagac gaattcggc cacagcgct cgattttgt 
ctttcggac tggaatgat cttctggag cgacgtcac 
ggacaaaaa gatatacaa agctccgat tgatgaagg 
aaatgagag gtcggtcgt gatcagtaa tgacataac 
gacttcaga cggcctcgc ggttgactg gatctacaa 
ccacatcta ttggacaga taccgggaa gaacacaat 
agaactggc gaatttcga tgggaaaat gcgcaaagt 
tttaatcaa agacgaatt agaagaacc cagagcgct 
cgcacttaa ccctttgga aggctggat gttctggac 
ggattgggg aagtgaccc aaaaattga aagatcggg 
catggacgg atcacacag acaggctat tgtaacata 
tgacgttaa atggcccaa tggattgac tctggattt 
agtcaaaaa acgggtcta ttgggtcga tgctaagct 
aaatgtgat ttcctcctg tgattataa tggaaggaa 
caggagact tatactctt ctcgccgga ttctctcag 
acatccatt ttcgatcac taccttcga agattgggt 
ctattggac cgactggga taaagccgc cgttttcaa 
agccaacaa attcaccgg aagcgacgt acaacccgt 
tacagccac tgctatgat ccagaaccc gatggtcat 
ccacgtcta ccaccctta ccgccagcc cgatgggga 
gaaccattg ccaggcggt gaacgggca ttgttcgca 
cctgtgctt gcctgcgcc ccaaataaa cgcgaaatc 
tccgcgtat ttcctgtgc atgtcccga gaacttaag 
gatgttgaa agatggatt gacttgcgt tcaagacga 
aacatatac gaccaccgt aaattctat aacttatac 
agattattc atcaatgtc agattatat gactgaaag 
tacaactaa caagtcgca cacaactaa cgtagtccc 
tgaaagttc cgatgacgg tgctgttgc tgttattgt 
aattggaat atcttttgc cgtgatagt cataatcgg 
tgttgtggt gtacttcct gtatagaaa atatgtgaa 
acgtaacat gacgtctat gaatttcga taatcctgt 
ttatcgtaa aacaaccga agatcaatt ttccttaga 
gaaaaacca atatccgcc atcccgacc gtatttaag 
tacagttgg tgaagaggc tcaacagcc tctaacagg 
tgccaacaa ccaaaatga caatgttta aacagtgaa 
attttttta ctcaaagtg ctcaattac agtgaaatt 
aaagactta gatttaatt tgacaaagt catcatttc 
tttttgtat agatgtgta tttattgta ccatcgtaa 
aaagaacta actattagt gtgttgtta aaattccag 
tttcgtgtc aaaccacag actgaatta agttcattg 
cgaaagtcc agaggaatt tgtcaaaat ttttcgtaa 
actgatttc ctaatttac tatttgtga tctcagtgg 
aaaatggga ttttaacga tttggatga aagtgagtg 
agattcgag aggtatggt taatttgtc caaagagtc 
gtattagca attaatcta agccaattg taattttta 
atagtcacg ttttctcca atgttaagt ttatttttc 
gaattcgta cctcgatat ttttaattt atctatttt 
cattgctca tgtttgtat atattaaat tttttagaa 
atgtttgta ttgttaaat tatttatta cactacgat 
gttaagctt gtaactatt ttactgcga gaaatcaaa 
atgtaaaaa gttgtatat ttcagtcat aaacatata 
ctaatgtag ctctaaaat tctgtgatt atattgttt 
gtattcctt ttcaatttt ctaggaatt tacattttt 
actgtctag attatttat cacatccta actttttgt 
tttatttgt gtaattatt aaaatttaa ataaatata 
tgtacaaga t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY