Status
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Gene information
General information
TC001977 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450289.1: 59315..64957
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001977
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03282

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (died as pupa) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001977 RA
      MNIPSKLKP CHPFDILKR IREPAICLA ISVPLPIRN 
      ALASARTDA SPSSTKWYP IDEVFSSSS KVLVLSLQM 
      FGQRGLFSD KMGTAQSVP IKKLDEIRF HLHDTEHVV 
      KTDSINPDT TLNSYLRQN LNLTGTKAM CHEGGCGSC 
      VVVLQKRDP LTQKDSFLA VNSCLIPIL SCNGWRIYT 
      VEGIGSPLV GYHPVQQIL AKFNGTQCG FCSPGMVMN 
      MYALYESGK LTKEEVENS FGGNICRCT GYRSILAAF 
      KSLCTDACP EMRSPDIED LRVCQRKNC EKKCVEILE 
      EPFYHLVGG SRWIKVYTL RDLFSTLYS YSSLNYKLV 
      AGNTAQGVF KTYSQPVDL YVDVTSIPE LTSQDFKNN 
      SLVLGANTT LTNAIEIFT ETSRKNPNF VYLKQLAQH 
      IDLVANVPV RNKGTLAGN LMMKHDHND FPSDVFLIL 
      ETVGVQFTI ALINGQETT LSPLDFIKS DMKLKILQN 
      IIFPEFASN VKFVSYKIM PRAQNTHAH VNAGFLFKF 
      DKDLIQEAR IIYGNINPT FVHATETEK FLVGKHLFD 
      NSVLQQAYG ILSKELDPN LIPPDPSPE FRKKLAVAL 
      FYKAILSIA PSDKTTLKN KSGGSLLQR PISKGVQDY 
      DTKKSLYPL TQPIPKLEA LAQTSGQAQ YIDDMPDLP 
      NQLFGALVL AESPPNSII KNINPKKAL EQDDIVAFF 
      SKDDIPGDN NFTPLNIAY IVAKEEIFC SGRVQYYEQ 
      PLGILVGKN FQAVQAAVK LVEVTYDGP NVEPLLSVR 
      QILKAGRKD RILETKTIK PKRRGNDIK HVIKGTFDI 
      HHQYHFHME TQCCNVVPT EDGLDIYPS SQWMDLTQV 
      SAANMLKIP NNKINVFVR RCGGAFGAK ISRNGLVSC 
      AAALASWKL RKPVKLSLP LSTNIAAIG KRWPLSTDY 
      EVGVDDKGV IQYLDCTHY SDVGAISNE DGTGELLNL 
      FMASYNPET FHIQMNKAI TDTHTNTWA RAPGTTEGL 
      AAIEAIIEH ISYVVNVDP LQVRLANFP KNSPLVKYV 
      NDIKSWANL DQRKKEIET FNQNNRWKK KGLAVVPMD 
      YELNLAGPF ATTVSIFHG DGSVQISHG GVEIGQGIN 
      TKAAQVCAY KLGIPLEKV SVIPSNSFV APNSMLTGS 
      SITSEAVCY GVIQACDQL LARIEPYRE QSGKVTWEE 
      LIQKCFEDY VNLSASGQF SPKEPNVAS YPIYGICAC 
      EVLVDILTG QHIVSRVDL VEDTGQSMS PEIDIGQIE 
      GAFVMGMGY YTMEHIVFN YEGKILTNN TWTYHPPGA 
      KDIPVEFNV KFPKNNPNP VGVLKSKAT GEPAVCLTI 
      AVPLAIRNA VASARLDAD AANTKWFPF DGPTNVENV 
      FMNSSNSYS QYVL
    CDS sequence
    • >TC001977 RA
      atgaacatt ccgtcaaaa ctgaaaccg tgtcacccc 
      ttcgacatc cttaaacga atacgagaa cctgccata 
      tgcctggca atatccgtg cccttgcca atccgaaac 
      gctttagcc tctgctcgc accgacgct agcccttct 
      agcaccaag tggtacccc atagatgaa gtcttcagt 
      agttcttcc aaagttcta gtactttct ctccagatg 
      ttcgggcaa cgtggtttg tttagtgac aaaatggga 
      acagcacaa tcggttccc ataaaaaaa cttgatgaa 
      atcaggttt catctgcac gacaccgag catgtcgtc 
      aaaacagat agcatcaac cctgacacc actttaaac 
      tcgtacctg cgacaaaat ttaaactta accggaact 
      aaagcaatg tgccatgag ggcggttgc ggctcttgt 
      gtcgttgtt ttgcaaaaa agggaccca ctcacccaa 
      aaagactca tttttggct gtcaattct tgcctgatt 
      ccaattttg tcatgtaac ggttggagg atttacaca 
      gttgagggt atagggagc ccacttgtg ggctaccac 
      cctgttcag caaatccta gctaaattc aacggcact 
      caatgcggt ttttgctcc cctggcatg gttatgaat 
      atgtacgct ttatacgag tcgggaaaa ttgacaaag 
      gaggaagtc gagaattcg tttgggggg aacatttgc 
      aggtgcacg gggtacagg tccatactt gcggctttt 
      aagtctctt tgcactgat gcgtgtccc gaaatgcgg 
      tccccggac attgaggat ttgagggtt tgtcagcga 
      aaaaattgc gaaaagaaa tgtgtcgag attttggag 
      gagccgttc tatcacctc gtggggggt tcaagatgg 
      attaaagtg tacactttg agggattta ttttcaacg 
      ttgtattct tactcgagt ttgaattat aaactagtt 
      gctgggaac acagcccaa ggagtgttc aaaacttac 
      tcacaacct gtcgatctt tacgtagat gtaacttca 
      attcctgaa cttacatcc caagacttt aaaaataat 
      tcacttgtt ttgggggct aacactaca cttacaaat 
      gcaatagaa atttttacc gaaacgtca agaaaaaat 
      cctaatttt gtttacttg aaacagttg gcccaacat 
      attgattta gtagctaat gttccagtg agaaataaa 
      ggtacttta gctggaaat ttaatgatg aaacatgat 
      cataacgac tttccttcg gatgttttt ctaatactg 
      gaaacagtg ggtgtccag ttcactatt gctttaata 
      aatgggcaa gagacgacg ttaagtcct ctcgatttt 
      attaaaagt gacatgaaa ttaaaaatt ctgcaaaat 
      attatcttt cccgagttt gcaagtaat gttaagttt 
      gtgagctac aagataatg cccagagcc cagaatacg 
      cacgcacat gtcaacgcc ggttttctg tttaaattt 
      gataaagat ttaatacaa gaagctcgc atcatttat 
      ggaaatatc aaccccacc tttgttcac gccactgaa 
      actgagaaa tttctagtg ggcaaacat ttgtttgac 
      aatagcgtg ttgcaacaa gcttatggt attttgtca 
      aaagagttg gatccaaac ttgatacca cccgatcct 
      agtccagaa tttcgaaaa aaattggcc gtagcatta 
      ttttataaa gcaattttg agtattgcg ccgtctgat 
      aaaacaaca ctaaaaaat aaatcgggt ggttcactg 
      ttgcaacgg ccaatttca aaaggagta caagactac 
      gacaccaag aagtctttg tatcctctc actcaacca 
      attccaaaa ttagaagcc ctagcccag acatcaggt 
      caagcccaa tacattgac gacatgcca gacctaccc 
      aaccaatta tttggtgcc ctggtactt gcagaatca 
      cctccaaac tcaataatc aaaaacatc aaccccaaa 
      aaagcgcta gaacaagac gacattgtt gcttttttc 
      agcaaagac gatattccc ggtgacaac aacttcacc 
      cctctcaat atcgcttat atcgtagca aaggaagaa 
      attttttgc agtggcaga gttcaatat tacgaacaa 
      ccattgggc attttagtt ggcaaaaat tttcaagcc 
      gtccaagcg gctgttaag ctcgtggaa gtgacttat 
      gacgggcca aatgttgaa ccgttacta tcagtacgc 
      caaattttg aaagcgggc cgaaaagac cgaattcta 
      gaaaccaag acaatcaaa ccgaaacga cgcggcaat 
      gacatcaaa cacgtaata aaagggact tttgatatc 
      caccaccag taccatttc catatggaa actcaatgt 
      tgcaatgtt gtaccaacc gaggatggg cttgatatc 
      tacccctca agccagtgg atggatttg acccaagtc 
      tcggccgca aacatgctc aaaatccca aataacaaa 
      atcaatgtt tttgtgcgc cgatgtggg ggcgctttc 
      ggggccaaa atcagtcga aatgggctg gtttcatgt 
      gctgctgct ttggcgtct tggaaatta cgaaaaccg 
      gtcaaatta tcgctgcct ttgagcacc aacatcgcc 
      gcaatcggc aaacggtgg ccgctttca actgactac 
      gaagtcggt gttgatgat aagggggtc atacagtac 
      cttgactgc acccactat tctgatgtt ggcgccata 
      agtaacgag gatggtaca ggcgaactt ttaaacctg 
      ttcatggct agttataac cctgagact tttcacatt 
      caaatgaat aaagcaatc acagacact catactaat 
      acctgggct agggcccca gggaccact gaaggcctt 
      gcggcaatt gaggccata attgagcac atttcctat 
      gttgtcaat gttgatccc ttacaagta cggcttgca 
      aattttcca aaaaactca ccgcttgta aaatatgtc 
      aatgacatt aaatcgtgg gctaatctt gatcagaga 
      aaaaaagaa attgagact tttaaccag aataacaga 
      tggaagaaa aagggcctt gctgtggtc cccatggac 
      tacgaattg aacctagct ggtcccttt gctaccaca 
      gtgtctatt ttccacggc gatggctcc gtccagata 
      tcacacgga ggggttgaa attggacaa ggaattaac 
      acaaaagct gcacaagtg tgtgcctac aaactaggc 
      atccctctt gagaaggtt tcggtgatt ccgagtaac 
      agtttcgtg gcacctaac tcaatgctg accggtagt 
      agcatcact tctgaggca gtttgctat ggagttatt 
      caagcttgt gaccaactc ctggcccga attgaaccg 
      tatcgcgag cagtctgga aaagtgact tgggaagag 
      ctaatccag aagtgtttc gaagactat gtcaaccta 
      tcggcgagt ggccagttt tcgcccaaa gagccaaac 
      gtggcctcg taccccatc tacggtatc tgtgcgtgt 
      gaagttttg gtcgacatt ttgacaggg caacacata 
      gtgtcccga gtcgaccta gtcgaagac accggacag 
      tcaatgagc cccgaaatc gacattggg caaatcgag 
      ggcgccttt gttatggga atggggtac tacacaatg 
      gaacacatt gtttttaat tacgagggc aaaattttg 
      accaacaac acttggact tatcatccc ccgggtgct 
      aaggacatc ccggttgag tttaacgtc aagtttccg 
      aaaaataat cccaaccct gttggagtc ctaaagtcg 
      aaagcgacc ggcgagcct gcggtttgt ctcacaata 
      gcagttcct ttggcaatt cgaaacgct gtggcctct 
      gctagactc gacgcagat gctgccaat acaaagtgg 
      tttcccttt gatgggcca actaatgtc gaaaatgtt 
      ttcatgaac agctcgaat agttactcg caatatgtt 
      ttgtga
    mRNA sequence
    • >TC001977 RA
      ctcctgtaa taattgttg ttttttaat aaaaatggg 
      agccgcaca gtcgatccc gccagaaaa taaagtgaa 
      aagaatcaa gacaataaa attttattt gcaagacaa 
      tgaacattc cgtcaaaac tgaaaccgt gtcacccct 
      tcgacatcc ttaaacgaa tacgagaac ctgccatat 
      gcctggcaa tatccgtgc ccttgccaa tccgaaacg 
      ctttagcct ctgctcgca ccgacgcta gcccttcta 
      gcaccaagt ggtacccca tagatgaag tcttcagta 
      gttcttcca aagttctag tactttctc tccagatgt 
      tcgggcaac gtggtttgt ttagtgaca aaatgggaa 
      cagcacaat cggttccca taaaaaaac ttgatgaaa 
      tcaggtttc atctgcacg acaccgagc atgtcgtca 
      aaacagata gcatcaacc ctgacacca ctttaaact 
      cgtacctgc gacaaaatt taaacttaa ccggaacta 
      aagcaatgt gccatgagg gcggttgcg gctcttgtg 
      tcgttgttt tgcaaaaaa gggacccac tcacccaaa 
      aagactcat ttttggctg tcaattctt gcctgattc 
      caattttgt catgtaacg gttggagga tttacacag 
      ttgagggta tagggagcc cacttgtgg gctaccacc 
      ctgttcagc aaatcctag ctaaattca acggcactc 
      aatgcggtt tttgctccc ctggcatgg ttatgaata 
      tgtacgctt tatacgagt cgggaaaat tgacaaagg 
      aggaagtcg agaattcgt ttgggggga acatttgca 
      ggtgcacgg ggtacaggt ccatacttg cggctttta 
      agtctcttt gcactgatg cgtgtcccg aaatgcggt 
      ccccggaca ttgaggatt tgagggttt gtcagcgaa 
      aaaattgcg aaaagaaat gtgtcgaga ttttggagg 
      agccgttct atcacctcg tggggggtt caagatgga 
      ttaaagtgt acactttga gggatttat tttcaacgt 
      tgtattctt actcgagtt tgaattata aactagttg 
      ctgggaaca cagcccaag gagtgttca aaacttact 
      cacaacctg tcgatcttt acgtagatg taacttcaa 
      ttcctgaac ttacatccc aagacttta aaaataatt 
      cacttgttt tgggggcta acactacac ttacaaatg 
      caatagaaa tttttaccg aaacgtcaa gaaaaaatc 
      ctaattttg tttacttga aacagttgg cccaacata 
      ttgatttag tagctaatg ttccagtga gaaataaag 
      gtactttag ctggaaatt taatgatga aacatgatc 
      ataacgact ttccttcgg atgtttttc taatactgg 
      aaacagtgg gtgtccagt tcactattg ctttaataa 
      atgggcaag agacgacgt taagtcctc tcgatttta 
      ttaaaagtg acatgaaat taaaaattc tgcaaaata 
      ttatctttc ccgagtttg caagtaatg ttaagtttg 
      tgagctaca agataatgc ccagagccc agaatacgc 
      acgcacatg tcaacgccg gttttctgt ttaaatttg 
      ataaagatt taatacaag aagctcgca tcatttatg 
      gaaatatca accccacct ttgttcacg ccactgaaa 
      ctgagaaat ttctagtgg gcaaacatt tgtttgaca 
      atagcgtgt tgcaacaag cttatggta ttttgtcaa 
      aagagttgg atccaaact tgataccac ccgatccta 
      gtccagaat ttcgaaaaa aattggccg tagcattat 
      tttataaag caattttga gtattgcgc cgtctgata 
      aaacaacac taaaaaata aatcgggtg gttcactgt 
      tgcaacggc caatttcaa aaggagtac aagactacg 
      acaccaaga agtctttgt atcctctca ctcaaccaa 
      ttccaaaat tagaagccc tagcccaga catcaggtc 
      aagcccaat acattgacg acatgccag acctaccca 
      accaattat ttggtgccc tggtacttg cagaatcac 
      ctccaaact caataatca aaaacatca accccaaaa 
      aagcgctag aacaagacg acattgttg cttttttca 
      gcaaagacg atattcccg gtgacaaca acttcaccc 
      ctctcaata tcgcttata tcgtagcaa aggaagaaa 
      ttttttgca gtggcagag ttcaatatt acgaacaac 
      cattgggca ttttagttg gcaaaaatt ttcaagccg 
      tccaagcgg ctgttaagc tcgtggaag tgacttatg 
      acgggccaa atgttgaac cgttactat cagtacgcc 
      aaattttga aagcgggcc gaaaagacc gaattctag 
      aaaccaaga caatcaaac cgaaacgac gcggcaatg 
      acatcaaac acgtaataa aagggactt ttgatatcc 
      accaccagt accatttcc atatggaaa ctcaatgtt 
      gcaatgttg taccaaccg aggatgggc ttgatatct 
      acccctcaa gccagtgga tggatttga cccaagtct 
      cggccgcaa acatgctca aaatcccaa ataacaaaa 
      tcaatgttt ttgtgcgcc gatgtgggg gcgctttcg 
      gggccaaaa tcagtcgaa atgggctgg tttcatgtg 
      ctgctgctt tggcgtctt ggaaattac gaaaaccgg 
      tcaaattat cgctgcctt tgagcacca acatcgccg 
      caatcggca aacggtggc cgctttcaa ctgactacg 
      aagtcggtg ttgatgata agggggtca tacagtacc 
      ttgactgca cccactatt ctgatgttg gcgccataa 
      gtaacgagg atggtacag gcgaacttt taaacctgt 
      tcatggcta gttataacc ctgagactt ttcacattc 
      aaatgaata aagcaatca cagacactc atactaata 
      cctgggcta gggccccag ggaccactg aaggccttg 
      cggcaattg aggccataa ttgagcaca tttcctatg 
      ttgtcaatg ttgatccct tacaagtac ggcttgcaa 
      attttccaa aaaactcac cgcttgtaa aatatgtca 
      atgacatta aatcgtggg ctaatcttg atcagagaa 
      aaaaagaaa ttgagactt ttaaccaga ataacagat 
      ggaagaaaa agggccttg ctgtggtcc ccatggact 
      acgaattga acctagctg gtccctttg ctaccacag 
      tgtctattt tccacggcg atggctccg tccagatat 
      cacacggag gggttgaaa ttggacaag gaattaaca 
      caaaagctg cacaagtgt gtgcctaca aactaggca 
      tccctcttg agaaggttt cggtgattc cgagtaaca 
      gtttcgtgg cacctaact caatgctga ccggtagta 
      gcatcactt ctgaggcag tttgctatg gagttattc 
      aagcttgtg accaactcc tggcccgaa ttgaaccgt 
      atcgcgagc agtctggaa aagtgactt gggaagagc 
      taatccaga agtgtttcg aagactatg tcaacctat 
      cggcgagtg gccagtttt cgcccaaag agccaaacg 
      tggcctcgt accccatct acggtatct gtgcgtgtg 
      aagttttgg tcgacattt tgacagggc aacacatag 
      tgtcccgag tcgacctag tcgaagaca ccggacagt 
      caatgagcc ccgaaatcg acattgggc aaatcgagg 
      gcgcctttg ttatgggaa tggggtact acacaatgg 
      aacacattg tttttaatt acgagggca aaattttga 
      ccaacaaca cttggactt atcatcccc cgggtgcta 
      aggacatcc cggttgagt ttaacgtca agtttccga 
      aaaataatc ccaaccctg ttggagtcc taaagtcga 
      aagcgaccg gcgagcctg cggtttgtc tcacaatag 
      cagttcctt tggcaattc gaaacgctg tggcctctg 
      ctagactcg acgcagatg ctgccaata caaagtggt 
      ttccctttg atgggccaa ctaatgtcg aaaatgttt 
      tcatgaaca gctcgaata gttactcgc aatatgttt 
      tgtgaaatt tgtttatgc actgataaa agatttcta 
      actagtgga gttatcagt gaaatattt attttcact 
      tgtttgcaa aaaatacaa gtaaactaa taaaattgt 
      ttgttttat
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