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Gene information
General information
TC001970 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid161: 52604..55444
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001970
    iBeetle screen iB_03281
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001970 RA
      MGVTQLLPF LAKSTRPCH ISEFRGRTV AIDSYCWLH 
      RGARGCAMQ LAKNEDVDS YVHYCMKYI KTLLSHDIR 
      PIMVFDGRH LPAKMKTEE KRRESKKLA RKQGMECLR 
      LGHTSDAYK FFAQSIDVT PEMALKLIR ECRKLHIDC 
      IVAPYEADA QLAYLNIKG FADCVITED SDLLLFGCL 
      KVMYKMDSH GQGQLVEAD KIHISMKMK QACFTMEKF 
      RYMCILSGC DYLDSLPGI GLRKALKFI SMTAETNPN 
      IFLDKLARY LKMPSLIVT DEYKQNFLV ANATFLHQI 
      VFDPIQRKL VPLTPVTTD VDYCQNAGE FFDPNTAFQ 
      LALGNLNPL TLQPIDNWT PTKGIVSDF SIWSQRFRF 
      RSPPRRAAP AASASISTP LFKFETFDR AAIEASLAQ 
      HDQEVEKEL AFYNMPPPA SEQKMDVDS IESRESSSS 
      GSPVLNKNP FMKKKLSKF QSTSASMPR VVSRFFSSS 
      DGSENVDSS PSSVQTPES TSPVILTPD SGSPSEGIS 
      TLVECECIL EVEDSVLLS VENLPNTER KRRSEDEHG 
      GKPGKKAKR QPTIQEMFK KMQTR
    CDS sequence
    • >TC001970 RA
      atgggagtt acacaactc ttgcccttc ctggcgaag 
      agcacccgg ccttgccat atctccgag tttcggggg 
      cgcacagtg gccatcgac agctactgc tggcttcat 
      cgaggtgcc cgaggctgt gccatgcaa ttggcaaag 
      aacgaggac gtcgattcg tacgtgcac tactgcatg 
      aagtacatc aagacgctc ctcagccat gacattaga 
      cctataatg gtgtttgac ggtcggcac ttgccggct 
      aaaatgaag actgaagaa aaaagacgc gaatcgaaa 
      aaactggcc cgaaaacag ggcatggag tgtctcaga 
      ttggggcac accagcgac gcttacaag tttttcgcg 
      cagtcgatc gacgtcacc cccgaaatg gccctaaag 
      cttattaga gagtgcagg aaactgcat attgattgc 
      attgtcgcc ccctatgag gccgacgcc cagttggcc 
      tacttgaac ataaagggg ttcgcggac tgcgttata 
      accgaggac tccgacttg ctcttgttc ggatgcctc 
      aaagtcatg tataagatg gattcacat gggcagggg 
      caacttgtt gaggccgac aagatccac atttcaatg 
      aaaatgaag caagcgtgc ttcactatg gagaagttt 
      cgttacatg tgcattttg tcaggctgc gattattta 
      gattctctt ccgggaatc gggcttcga aaggccttg 
      aaatttata tccatgacg gcggagact aacccgaat 
      atattttta gacaaatta gcgcgctac ttgaagatg 
      ccttcactc attgtgact gatgagtat aaacagaat 
      ttcctagtc gctaacgca acatttttg catcagatt 
      gtatttgac ccaattcaa aggaaatta gtccctttg 
      acgccggtc actaccgat gttgactac tgtcaaaac 
      gcaggggaa ttttttgac ccaaacacg gccttccaa 
      ttggctttg ggcaacctg aatcccctc acgttgcag 
      ccaatagat aactggact cccaccaaa ggcattgtg 
      agtgatttc agtatttgg tcgcaaagg ttccgattc 
      cgctctccg cctcgtcga gccgcacca gcggcaagt 
      gcgtcgatt tcaacgccc ttgttcaag ttcgaaacc 
      tttgatcgc gctgccatt gaagcatca ttggcgcaa 
      cacgaccaa gaagtagag aaagagctc gctttctac 
      aacatgcct ccacccgcc agtgagcaa aaaatggat 
      gtggacagc atcgaatct cgcgagtcg agctcttcc 
      ggctcgccc gtcttgaat aagaaccca tttatgaaa 
      aagaaactc agcaaattt cagtcgact agcgcgagc 
      atgccgaga gttgttagt cgcttcttc tcgtcatct 
      gatggctcg gagaatgtc gatagctcc ccctcgtcc 
      gtgcaaact cccgaaagt actagtcct gtcattttg 
      acgcctgat agtggtagc ccttccgaa ggcatttcg 
      acgcttgta gaatgcgaa tgcattcta gaagtggaa 
      gactcggtt ttgctcagc gtcgaaaat ttgccaaat 
      actgagcgc aagcggcgc agtgaagat gaacatggt 
      ggcaaaccg ggaaaaaag gccaaaaga caacctacg 
      attcaagaa atgttcaag aaaatgcag actcgttaa
    mRNA sequence
    • >TC001970 RA
      ggcggcgcc acctctgtt tgtaaacaa catttggag 
      ggaaagtag tagaaatgt gagctgagt ttttagctg 
      cttattgcc ctttagaat ctttagatt gctcaaaaa 
      atttgtgtt agtgtttcg ttagtattc cttagtgtt 
      agattcaac aattaaata gtcgcaatg ggagttaca 
      caactcttg cccttcctg gcgaagagc acccggcct 
      tgccatatc tccgagttt cgggggcgc acagtggcc 
      atcgacagc tactgctgg cttcatcga ggtgcccga 
      ggctgtgcc atgcaattg gcaaagaac gaggacgtc 
      gattcgtac gtgcactac tgcatgaag tacatcaag 
      acgctcctc agccatgac attagacct ataatggtg 
      tttgacggt cggcacttg ccggctaaa atgaagact 
      gaagaaaaa agacgcgaa tcgaaaaaa ctggcccga 
      aaacagggc atggagtgt ctcagattg gggcacacc 
      agcgacgct tacaagttt ttcgcgcag tcgatcgac 
      gtcaccccc gaaatggcc ctaaagctt attagagag 
      tgcaggaaa ctgcatatt gattgcatt gtcgccccc 
      tatgaggcc gacgcccag ttggcctac ttgaacata 
      aaggggttc gcggactgc gttataacc gaggactcc 
      gacttgctc ttgttcgga tgcctcaaa gtcatgtat 
      aagatggat tcacatggg caggggcaa cttgttgag 
      gccgacaag atccacatt tcaatgaaa atgaagcaa 
      gcgtgcttc actatggag aagtttcgt tacatgtgc 
      attttgtca ggctgcgat tatttagat tctcttccg 
      ggaatcggg cttcgaaag gccttgaaa tttatatcc 
      atgacggcg gagactaac ccgaatata tttttagac 
      aaattagcg cgctacttg aagatgcct tcactcatt 
      gtgactgat gagtataaa cagaatttc ctagtcgct 
      aacgcaaca tttttgcat cagattgta tttgaccca 
      attcaaagg aaattagtc cctttgacg ccggtcact 
      accgatgtt gactactgt caaaacgca ggggaattt 
      tttgaccca aacacggcc ttccaattg gctttgggc 
      aacctgaat cccctcacg ttgcagcca atagataac 
      tggactccc accaaaggc attgtgagt gatttcagt 
      atttggtcg caaaggttc cgattccgc tctccgcct 
      cgtcgagcc gcaccagcg gcaagtgcg tcgatttca 
      acgcccttg ttcaagttc gaaaccttt gatcgcgct 
      gccattgaa gcatcattg gcgcaacac gaccaagaa 
      gtagagaaa gagctcgct ttctacaac atgcctcca 
      cccgccagt gagcaaaaa atggatgtg gacagcatc 
      gaatctcgc gagtcgagc tcttccggc tcgcccgtc 
      ttgaataag aacccattt atgaaaaag aaactcagc 
      aaatttcag tcgactagc gcgagcatg ccgagagtt 
      gttagtcgc ttcttctcg tcatctgat ggctcggag 
      aatgtcgat agctccccc tcgtccgtg caaactccc 
      gaaagtact agtcctgtc attttgacg cctgatagt 
      ggtagccct tccgaaggc atttcgacg cttgtagaa 
      tgcgaatgc attctagaa gtggaagac tcggttttg 
      ctcagcgtc gaaaatttg ccaaatact gagcgcaag 
      cggcgcagt gaagatgaa catggtggc aaaccggga 
      aaaaaggcc aaaagacaa cctacgatt caagaaatg 
      ttcaagaaa atgcagact cgttaaaca tttcttcta 
      gttccttaa attaccaaa gagctttaa atcaaagta 
      ttattccat ttttagttt ttagtctta agacttgtg 
      atgatatta ttttgtgat ttacttttt tgcattttt 
      cactttttg aaagttttt ggtattatt cgttcaaat 
      cattttaat agttgctaa acagtggtt atgtactcc 
      aatattgaa atttcactc cactcgttt tcattgcag 
      ttttaataa attaactat gtatttgtt ccagttact 
      catgccaaa ggagtttag gctaagaat tttgttaca 
      ttaatatat attttttta atcgttcga atgttccac 
      gttttgaaa gtttttaaa taaattaat ctttgaaat