Status
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Gene information
General information
TC001965 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NW_015450289.1: 163712..168463
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001965
    iBeetle screen iB_03279

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001965 RA
      MSSVSEYNS AFKSLALVL LIIPICVFT SELNDVWMR 
      NVSKELGED LGIIKNEEL GISYIKKLY KTFNYTSAI 
      IDANKTVNE IVYKLERKI DDIFSALLT TKAAIQNLQ 
      MLNYTLKIR STLLPCIKN QTDKSEEAI ISLLDKIKT 
      HVITKNDTK QQYFISTSD LQCHYSPFI NCERIDHML 
      LWKNFMSRN NYASKNIVL LLDHGGSLN KRHFAIVLS 
      IAKQIVENL DEDDKIAIL AISNDWSSP LLNEECPVT 
      NQVPSVVDD VPNFTHVTA SRKKLLYNF IDGMHKGSG 
      ATNHSLGLQ KALKVIENS QIQNETVMI LYISRGLLS 
      SLTEAKAVL ETIVEASQN ISCPFVINT CAVIDDSRP 
      TIYETQFLE DIALQNYQK YNISHPGNV FRGIMVPIN 
      STETVGFAL ARFYMDYNQ NYNLQKLIS LPTWDERSN 
      DLTISFSLG YLHNSNFGL LGLDVYFAS WARELIYFS 
      NSHKNYYAF LTDMAGRVI LHPKFASVM SVKRQLQYV 
      DISYFENVA YPHIVKKRL LNEREGFQL TTQFGSPVK 
      WTWKRVGDW FIVCIVTHE SPTPLKPHH ITWPFNTSD 
      DYSIRLVYR HLLPSESNL CRHFNQAAT LDAGSLYLS 
      SSCFQSEKK TIEEDLHIT KNNTQQLED LGLKPEIKD 
      EVAALAYIL SFLRSQHLS SFKSKYIVR RYVASYSGV 
      LQMFPGSIL DQGLDPTKR AWFVQAVEH KSKIIMIPP 
      YLDEGGAGY IVTIAYATS DVVVALDVS YGFVFRTLL 
      KRMPFCLAT NVTCFLMDD RGYLIYHPN LINPNEHGP 
      VEKQHIVHK ESLVANDIL SHKYFIKKV LCNSYGSGT 
      VQRYYKLNT SYSDVLVSS VQGEHCVSY HIAAIRNSN 
      VFVGMVNAT CNVVVTFCP CSIVDRLCL NCNRMEQKE 
      CECPCECPL EQLKCTDYN HSNNPVCNW FPEHVTLKA 
      RFVEDATSE MKSCFPASC KREKTHLRC LGVTGCEWC 
      KYDIDGSPL ESPFCASMA TCFNGIIGS VTPYRNSLH 
      EIDLPEESF SVPISVITL FIFGVLLLL CMFYVYHRS 
      LAPQATERL YLSSTQENH LRMSDLNLS DNYHAMGNH 
      RDKLLHEDK PDPISPYCV SSNYKRTTL AADSDHGYS 
      TMTQHDESE HMSLAPVEF DSLDDDLGS DSVSTHISV 
      PNKGHVEPA QFTCIPRNK CIVVPVTVH RNMETT
    CDS sequence
    • >TC001965 RA
      atgagtagt gttagtgaa tataatagt gcgtttaaa 
      agtttagct ttagttttg ttaattata ccaatctgt 
      gtctttaca agcgaatta aatgatgtg tggatgcga 
      aatgtttcg aaagaattg ggtgaagac ttgggtata 
      ataaaaaac gaggaattg gggattagt tacatcaag 
      aaattgtac aaaacgttt aattacacc tcggctatc 
      attgatgcc aacaagacg gtaaacgaa attgtttat 
      aaactagag aggaaaata gacgacatt ttttccgcg 
      ctactgacc actaaagcg gcaattcag aatttgcaa 
      atgctgaat tacacactc aaaatacgc tctactctc 
      ctcccatgt atcaaaaat caaacggat aagagtgaa 
      gaagcaata atatcattg ttggacaag attaaaacc 
      catgtgatt acaaagaat gacaccaaa cagcagtat 
      tttatttca acgtctgat ctccagtgc cattattcg 
      ccttttatt aattgcgag agaattgac cacatgttg 
      ttgtggaaa aatttcatg tctcgaaac aattatgct 
      tccaaaaac attgtcctg ttgctggac catgggggc 
      tcgctcaac aagcgccac tttgccata gttttatca 
      attgccaaa caaattgtt gaaaatttg gacgaagac 
      gataaaatc gccatactt gccatatca aacgactgg 
      tcatctccg ctcttaaat gaggagtgt ccggtgaca 
      aaccaagtt ccttcagtt gttgacgac gttccgaac 
      tttactcac gttaccgcc agtaggaag aagttattg 
      tacaatttt atcgacggc atgcacaaa gggagcggt 
      gccacaaac cactcgttg ggtctccag aaagcttta 
      aaagtaatc gagaacagc cagatacaa aacgaaact 
      gtgatgatt ctgtacata agccggggt ttactgtcg 
      tcgttgacg gaggccaaa gccgtgctt gagacaata 
      gtggaggct tcgcaaaat atctcctgt ccgtttgtt 
      atcaacacg tgtgccgtt attgacgat agtagaccc 
      actatttac gaaacgcaa ttcctcgaa gacattgcg 
      ttacaaaat taccaaaag tataacatc tcgcatccc 
      gggaacgta ttcagaggg attatggtg ccgattaat 
      tcgacggaa acggtgggc tttgccttg gctcgcttt 
      tacatggac tataatcaa aactataac ttgcaaaaa 
      ctgatttcg cttccaacg tgggatgaa agatcgaac 
      gacttgaca atctccttt tcgttgggg tacttacac 
      aacagtaat tttgggttg ttgggcctt gatgtttat 
      tttgccagt tgggccaga gaacttatt tatttcagc 
      aattcgcac aaaaactac tacgctttt ttgaccgat 
      atggcagga cgggttatc ttacatccg aaattcgca 
      tcagttatg tcagtaaaa cggcaatta cagtacgtc 
      gatatatca tatttcgaa aacgttgca tatccacat 
      attgttaaa aagagactt ttgaacgaa cgtgaaggg 
      tttcaatta actactcag tttggatca cctgtcaaa 
      tggacttgg aagcgagtg ggtgattgg tttatcgtg 
      tgcatagtc acacacgag tcaccaaca cctctcaag 
      cctcaccac atcacgtgg ccttttaat acgtcggac 
      gactacagt attagactt gtgtaccga catttattg 
      ccgtctgaa tcaaattta tgtcgccat ttcaaccaa 
      gctgcaact ctggacgct ggttccctc tacttgagt 
      tcgtcgtgc ttccaatcc gaaaaaaag acaatcgag 
      gaggacctc catataaca aaaaacaat actcagcaa 
      ttggaagat ttgggcttg aagcctgaa atcaaggac 
      gaagtggca gctcttgct tatattttg agttttttg 
      cgcagccaa cacttgtct agcttcaaa tcgaagtat 
      atagttcgc cggtatgtg gcctcctac agcggcgta 
      cttcaaatg tttccgggc agtatcctc gatcaaggt 
      ctagatccg acgaagcga gcctggttt gtgcaagct 
      gttgagcac aagtcgaag attattatg attccgccg 
      tatttggac gaaggcggc gctggttat attgtgaca 
      attgcctac gccacctcg gatgtggtt gtggcactt 
      gatgttagt tacggattt gtgttccgc acacttttg 
      aaacgtatg cccttttgt ttggccaca aatgtcact 
      tgttttctg atggatgat cgggggtac ttgatttat 
      catccgaat ttgataaat ccgaacgag cacgggccg 
      gttgagaag caacatatt gtgcataaa gaatcactg 
      gttgctaat gatattttg agccataaa tatttcatt 
      aagaaggtg ctatgtaac agttacggg agtggcacc 
      gtccagagg tactacaag ctaaatact agttacagc 
      gatgttttg gttagttcg gtccaggga gagcattgc 
      gtcagttac cacatagct gcgatacga aatagcaac 
      gttttcgtt gggatggtt aacgcgact tgtaacgtc 
      gtggttaca ttctgtccg tgtagtatc gtggaccgg 
      ttatgtctg aactgcaac cgcatggaa caaaaagaa 
      tgtgaatgt ccgtgcgag tgtccgtta gaacagctc 
      aaatgtacg gactataac cacagtaac aatccggtt 
      tgtaactgg tttccggag cacgtgact ctcaaagct 
      cgctttgtt gaggatgcc acaagtgag atgaagtca 
      tgctttccg gccagttgc aagcgcgag aaaactcat 
      ttgaggtgt cttggagta accggttgc gaatggtgc 
      aagtatgat attgatggt agtccactg gagagtccg 
      ttttgcgcc tccatggcc acttgtttc aatggaatc 
      atcgggtct gtgacaccc tacaggaac tccctccac 
      gaaattgat cttccagaa gagtccttc agtgtgccc 
      ataagtgtc ataactttg ttcatattc ggagtcctc 
      ctcctcctt tgtatgttc tacgtgtat catcgctct 
      ctggcgccg caagccacc gaaagattg tacctttcg 
      tccactcag gagaatcat ttacgaatg agtgatctt 
      aatttgagt gataattac cacgctatg ggcaaccat 
      agagataag ttgcttcac gaggacaag ccggatccg 
      atttcgcca tactgtgtg tcgagcaat tacaagaga 
      acgactttg gcggcggat tcggatcat ggctactcg 
      acgatgact caacatgac gagtccgag catatgtct 
      ttggctccg gttgagttt gattcgttg gatgacgat 
      ttggggtcg gacagtgtt tccactcat atcagtgtg 
      ccaaataag ggacatgtc gaaccagcg cagttcaca 
      tgtattccc agaaacaaa tgtattgtg gttccagtc 
      acggtccac agaaacatg gaaacgacg taa
    mRNA sequence
    • >TC001965 RA
      aacatcgcc gtcattttg tttgtttac tgtttagag 
      gtttaattc tatctgcct aaaaaatgg gtagataat 
      aagaaaatt cacagtaaa accagtaat aattcacta 
      aaatgggat gaggttgaa agttcggta gctccagca 
      tgaattagg gtgtcctct ttggctaaa ttcactata 
      taaccctta tcaataatg agtagtgtt agtgaatat 
      aatagtgcg tttaaaagt ttagcttta gttttgtta 
      attatacca atctgtgtc tttacaagc gaattaaat 
      gatgtgtgg atgcgaaat gtttcgaaa gaattgggt 
      gaagacttg ggtataata aaaaacgag gaattgggg 
      attagttac atcaagaaa ttgtacaaa acgtttaat 
      tacacctcg gctatcatt gatgccaac aagacggta 
      aacgaaatt gtttataaa ctagagagg aaaatagac 
      gacattttt tccgcgcta ctgaccact aaagcggca 
      attcagaat ttgcaaatg ctgaattac acactcaaa 
      atacgctct actctcctc ccatgtatc aaaaatcaa 
      acggataag agtgaagaa gcaataata tcattgttg 
      gacaagatt aaaacccat gtgattaca aagaatgac 
      accaaacag cagtatttt atttcaacg tctgatctc 
      cagtgccat tattcgcct tttattaat tgcgagaga 
      attgaccac atgttgttg tggaaaaat ttcatgtct 
      cgaaacaat tatgcttcc aaaaacatt gtcctgttg 
      ctggaccat gggggctcg ctcaacaag cgccacttt 
      gccatagtt ttatcaatt gccaaacaa attgttgaa 
      aatttggac gaagacgat aaaatcgcc atacttgcc 
      atatcaaac gactggtca tctccgctc ttaaatgag 
      gagtgtccg gtgacaaac caagttcct tcagttgtt 
      gacgacgtt ccgaacttt actcacgtt accgccagt 
      aggaagaag ttattgtac aattttatc gacggcatg 
      cacaaaggg agcggtgcc acaaaccac tcgttgggt 
      ctccagaaa gctttaaaa gtaatcgag aacagccag 
      atacaaaac gaaactgtg atgattctg tacataagc 
      cggggttta ctgtcgtcg ttgacggag gccaaagcc 
      gtgcttgag acaatagtg gaggcttcg caaaatatc 
      tcctgtccg tttgttatc aacacgtgt gccgttatt 
      gacgatagt agacccact atttacgaa acgcaattc 
      ctcgaagac attgcgtta caaaattac caaaagtat 
      aacatctcg catcccggg aacgtattc agagggatt 
      atggtgccg attaattcg acggaaacg gtgggcttt 
      gccttggct cgcttttac atggactat aatcaaaac 
      tataacttg caaaaactg atttcgctt ccaacgtgg 
      gatgaaaga tcgaacgac ttgacaatc tccttttcg 
      ttggggtac ttacacaac agtaatttt gggttgttg 
      ggccttgat gtttatttt gccagttgg gccagagaa 
      cttatttat ttcagcaat tcgcacaaa aactactac 
      gcttttttg accgatatg gcaggacgg gttatctta 
      catccgaaa ttcgcatca gttatgtca gtaaaacgg 
      caattacag tacgtcgat atatcatat ttcgaaaac 
      gttgcatat ccacatatt gttaaaaag agacttttg 
      aacgaacgt gaagggttt caattaact actcagttt 
      ggatcacct gtcaaatgg acttggaag cgagtgggt 
      gattggttt atcgtgtgc atagtcaca cacgagtca 
      ccaacacct ctcaagcct caccacatc acgtggcct 
      tttaatacg tcggacgac tacagtatt agacttgtg 
      taccgacat ttattgccg tctgaatca aatttatgt 
      cgccatttc aaccaagct gcaactctg gacgctggt 
      tccctctac ttgagttcg tcgtgcttc caatccgaa 
      aaaaagaca atcgaggag gacctccat ataacaaaa 
      aacaatact cagcaattg gaagatttg ggcttgaag 
      cctgaaatc aaggacgaa gtggcagct cttgcttat 
      attttgagt tttttgcgc agccaacac ttgtctagc 
      ttcaaatcg aagtatata gttcgccgg tatgtggcc 
      tcctacagc ggcgtactt caaatgttt ccgggcagt 
      atcctcgat caaggtcta gatccgacg aagcgagcc 
      tggtttgtg caagctgtt gagcacaag tcgaagatt 
      attatgatt ccgccgtat ttggacgaa ggcggcgct 
      ggttatatt gtgacaatt gcctacgcc acctcggat 
      gtggttgtg gcacttgat gttagttac ggatttgtg 
      ttccgcaca cttttgaaa cgtatgccc ttttgtttg 
      gccacaaat gtcacttgt tttctgatg gatgatcgg 
      gggtacttg atttatcat ccgaatttg ataaatccg 
      aacgagcac gggccggtt gagaagcaa catattgtg 
      cataaagaa tcactggtt gctaatgat attttgagc 
      cataaatat ttcattaag aaggtgcta tgtaacagt 
      tacgggagt ggcaccgtc cagaggtac tacaagcta 
      aatactagt tacagcgat gttttggtt agttcggtc 
      cagggagag cattgcgtc agttaccac atagctgcg 
      atacgaaat agcaacgtt ttcgttggg atggttaac 
      gcgacttgt aacgtcgtg gttacattc tgtccgtgt 
      agtatcgtg gaccggtta tgtctgaac tgcaaccgc 
      atggaacaa aaagaatgt gaatgtccg tgcgagtgt 
      ccgttagaa cagctcaaa tgtacggac tataaccac 
      agtaacaat ccggtttgt aactggttt ccggagcac 
      gtgactctc aaagctcgc tttgttgag gatgccaca 
      agtgagatg aagtcatgc tttccggcc agttgcaag 
      cgcgagaaa actcatttg aggtgtctt ggagtaacc 
      ggttgcgaa tggtgcaag tatgatatt gatggtagt 
      ccactggag agtccgttt tgcgcctcc atggccact 
      tgtttcaat ggaatcatc gggtctgtg acaccctac 
      aggaactcc ctccacgaa attgatctt ccagaagag 
      tccttcagt gtgcccata agtgtcata actttgttc 
      atattcgga gtcctcctc ctcctttgt atgttctac 
      gtgtatcat cgctctctg gcgccgcaa gccaccgaa 
      agattgtac ctttcgtcc actcaggag aatcattta 
      cgaatgagt gatcttaat ttgagtgat aattaccac 
      gctatgggc aaccataga gataagttg cttcacgag 
      gacaagccg gatccgatt tcgccatac tgtgtgtcg 
      agcaattac aagagaacg actttggcg gcggattcg 
      gatcatggc tactcgacg atgactcaa catgacgag 
      tccgagcat atgtctttg gctccggtt gagtttgat 
      tcgttggat gacgatttg gggtcggac agtgtttcc 
      actcatatc agtgtgcca aataaggga catgtcgaa 
      ccagcgcag ttcacatgt attcccaga aacaaatgt 
      attgtggtt ccagtcacg gtccacaga aacatggaa 
      acgacgtaa cagtagata atagcttac ttaataatg 
      cctgtgtat atttctgca ccagtatat ttatttccg 
      tagtgtact taattgtat attttgtaa aatttattt 
      attttaact gtagtttta cccttgtag cttaatttg 
      gtatccatc catcgtgcg catataaag gacttacaa 
      attttttac attacttaa ccgccacac gtccatgtt 
      tgtacaaaa tttgttata tttggactt tgcgataaa 
      ttgtttgag acattctgt gccttagct ccgtttcaa 
      tgtttgttt cctttgcca ctttcgagt aatttgtcg 
      gaaatgtcc cattttgac tgttattga aaaaattgt 
      tacggatta ttgtgcaat acaaacgtt caatcgtag 
      ctatccatt gcataaagt gtacctgtt tttaaggtt 
      tgtgtactt ttttaaaaa taaattctg ttcaattga
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY