| General information | |||
| TC001965 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NW_015450289.1: 163712..168463 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001965 | |
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03279 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 3 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001965 RA
MSSVSEYNS AFKSLALVL LIIPICVFT SELNDVWMR NVSKELGED LGIIKNEEL GISYIKKLY KTFNYTSAI IDANKTVNE IVYKLERKI DDIFSALLT TKAAIQNLQ MLNYTLKIR STLLPCIKN QTDKSEEAI ISLLDKIKT HVITKNDTK QQYFISTSD LQCHYSPFI NCERIDHML LWKNFMSRN NYASKNIVL LLDHGGSLN KRHFAIVLS IAKQIVENL DEDDKIAIL AISNDWSSP LLNEECPVT NQVPSVVDD VPNFTHVTA SRKKLLYNF IDGMHKGSG ATNHSLGLQ KALKVIENS QIQNETVMI LYISRGLLS SLTEAKAVL ETIVEASQN ISCPFVINT CAVIDDSRP TIYETQFLE DIALQNYQK YNISHPGNV FRGIMVPIN STETVGFAL ARFYMDYNQ NYNLQKLIS LPTWDERSN DLTISFSLG YLHNSNFGL LGLDVYFAS WARELIYFS NSHKNYYAF LTDMAGRVI LHPKFASVM SVKRQLQYV DISYFENVA YPHIVKKRL LNEREGFQL TTQFGSPVK WTWKRVGDW FIVCIVTHE SPTPLKPHH ITWPFNTSD DYSIRLVYR HLLPSESNL CRHFNQAAT LDAGSLYLS SSCFQSEKK TIEEDLHIT KNNTQQLED LGLKPEIKD EVAALAYIL SFLRSQHLS SFKSKYIVR RYVASYSGV LQMFPGSIL DQGLDPTKR AWFVQAVEH KSKIIMIPP YLDEGGAGY IVTIAYATS DVVVALDVS YGFVFRTLL KRMPFCLAT NVTCFLMDD RGYLIYHPN LINPNEHGP VEKQHIVHK ESLVANDIL SHKYFIKKV LCNSYGSGT VQRYYKLNT SYSDVLVSS VQGEHCVSY HIAAIRNSN VFVGMVNAT CNVVVTFCP CSIVDRLCL NCNRMEQKE CECPCECPL EQLKCTDYN HSNNPVCNW FPEHVTLKA RFVEDATSE MKSCFPASC KREKTHLRC LGVTGCEWC KYDIDGSPL ESPFCASMA TCFNGIIGS VTPYRNSLH EIDLPEESF SVPISVITL FIFGVLLLL CMFYVYHRS LAPQATERL YLSSTQENH LRMSDLNLS DNYHAMGNH RDKLLHEDK PDPISPYCV SSNYKRTTL AADSDHGYS TMTQHDESE HMSLAPVEF DSLDDDLGS DSVSTHISV PNKGHVEPA QFTCIPRNK CIVVPVTVH RNMETT
- >TC001965 RA
atgagtagt gttagtgaa tataatagt gcgtttaaa agtttagct ttagttttg ttaattata ccaatctgt gtctttaca agcgaatta aatgatgtg tggatgcga aatgtttcg aaagaattg ggtgaagac ttgggtata ataaaaaac gaggaattg gggattagt tacatcaag aaattgtac aaaacgttt aattacacc tcggctatc attgatgcc aacaagacg gtaaacgaa attgtttat aaactagag aggaaaata gacgacatt ttttccgcg ctactgacc actaaagcg gcaattcag aatttgcaa atgctgaat tacacactc aaaatacgc tctactctc ctcccatgt atcaaaaat caaacggat aagagtgaa gaagcaata atatcattg ttggacaag attaaaacc catgtgatt acaaagaat gacaccaaa cagcagtat tttatttca acgtctgat ctccagtgc cattattcg ccttttatt aattgcgag agaattgac cacatgttg ttgtggaaa aatttcatg tctcgaaac aattatgct tccaaaaac attgtcctg ttgctggac catgggggc tcgctcaac aagcgccac tttgccata gttttatca attgccaaa caaattgtt gaaaatttg gacgaagac gataaaatc gccatactt gccatatca aacgactgg tcatctccg ctcttaaat gaggagtgt ccggtgaca aaccaagtt ccttcagtt gttgacgac gttccgaac tttactcac gttaccgcc agtaggaag aagttattg tacaatttt atcgacggc atgcacaaa gggagcggt gccacaaac cactcgttg ggtctccag aaagcttta aaagtaatc gagaacagc cagatacaa aacgaaact gtgatgatt ctgtacata agccggggt ttactgtcg tcgttgacg gaggccaaa gccgtgctt gagacaata gtggaggct tcgcaaaat atctcctgt ccgtttgtt atcaacacg tgtgccgtt attgacgat agtagaccc actatttac gaaacgcaa ttcctcgaa gacattgcg ttacaaaat taccaaaag tataacatc tcgcatccc gggaacgta ttcagaggg attatggtg ccgattaat tcgacggaa acggtgggc tttgccttg gctcgcttt tacatggac tataatcaa aactataac ttgcaaaaa ctgatttcg cttccaacg tgggatgaa agatcgaac gacttgaca atctccttt tcgttgggg tacttacac aacagtaat tttgggttg ttgggcctt gatgtttat tttgccagt tgggccaga gaacttatt tatttcagc aattcgcac aaaaactac tacgctttt ttgaccgat atggcagga cgggttatc ttacatccg aaattcgca tcagttatg tcagtaaaa cggcaatta cagtacgtc gatatatca tatttcgaa aacgttgca tatccacat attgttaaa aagagactt ttgaacgaa cgtgaaggg tttcaatta actactcag tttggatca cctgtcaaa tggacttgg aagcgagtg ggtgattgg tttatcgtg tgcatagtc acacacgag tcaccaaca cctctcaag cctcaccac atcacgtgg ccttttaat acgtcggac gactacagt attagactt gtgtaccga catttattg ccgtctgaa tcaaattta tgtcgccat ttcaaccaa gctgcaact ctggacgct ggttccctc tacttgagt tcgtcgtgc ttccaatcc gaaaaaaag acaatcgag gaggacctc catataaca aaaaacaat actcagcaa ttggaagat ttgggcttg aagcctgaa atcaaggac gaagtggca gctcttgct tatattttg agttttttg cgcagccaa cacttgtct agcttcaaa tcgaagtat atagttcgc cggtatgtg gcctcctac agcggcgta cttcaaatg tttccgggc agtatcctc gatcaaggt ctagatccg acgaagcga gcctggttt gtgcaagct gttgagcac aagtcgaag attattatg attccgccg tatttggac gaaggcggc gctggttat attgtgaca attgcctac gccacctcg gatgtggtt gtggcactt gatgttagt tacggattt gtgttccgc acacttttg aaacgtatg cccttttgt ttggccaca aatgtcact tgttttctg atggatgat cgggggtac ttgatttat catccgaat ttgataaat ccgaacgag cacgggccg gttgagaag caacatatt gtgcataaa gaatcactg gttgctaat gatattttg agccataaa tatttcatt aagaaggtg ctatgtaac agttacggg agtggcacc gtccagagg tactacaag ctaaatact agttacagc gatgttttg gttagttcg gtccaggga gagcattgc gtcagttac cacatagct gcgatacga aatagcaac gttttcgtt gggatggtt aacgcgact tgtaacgtc gtggttaca ttctgtccg tgtagtatc gtggaccgg ttatgtctg aactgcaac cgcatggaa caaaaagaa tgtgaatgt ccgtgcgag tgtccgtta gaacagctc aaatgtacg gactataac cacagtaac aatccggtt tgtaactgg tttccggag cacgtgact ctcaaagct cgctttgtt gaggatgcc acaagtgag atgaagtca tgctttccg gccagttgc aagcgcgag aaaactcat ttgaggtgt cttggagta accggttgc gaatggtgc aagtatgat attgatggt agtccactg gagagtccg ttttgcgcc tccatggcc acttgtttc aatggaatc atcgggtct gtgacaccc tacaggaac tccctccac gaaattgat cttccagaa gagtccttc agtgtgccc ataagtgtc ataactttg ttcatattc ggagtcctc ctcctcctt tgtatgttc tacgtgtat catcgctct ctggcgccg caagccacc gaaagattg tacctttcg tccactcag gagaatcat ttacgaatg agtgatctt aatttgagt gataattac cacgctatg ggcaaccat agagataag ttgcttcac gaggacaag ccggatccg atttcgcca tactgtgtg tcgagcaat tacaagaga acgactttg gcggcggat tcggatcat ggctactcg acgatgact caacatgac gagtccgag catatgtct ttggctccg gttgagttt gattcgttg gatgacgat ttggggtcg gacagtgtt tccactcat atcagtgtg ccaaataag ggacatgtc gaaccagcg cagttcaca tgtattccc agaaacaaa tgtattgtg gttccagtc acggtccac agaaacatg gaaacgacg taa
- >TC001965 RA
aacatcgcc gtcattttg tttgtttac tgtttagag gtttaattc tatctgcct aaaaaatgg gtagataat aagaaaatt cacagtaaa accagtaat aattcacta aaatgggat gaggttgaa agttcggta gctccagca tgaattagg gtgtcctct ttggctaaa ttcactata taaccctta tcaataatg agtagtgtt agtgaatat aatagtgcg tttaaaagt ttagcttta gttttgtta attatacca atctgtgtc tttacaagc gaattaaat gatgtgtgg atgcgaaat gtttcgaaa gaattgggt gaagacttg ggtataata aaaaacgag gaattgggg attagttac atcaagaaa ttgtacaaa acgtttaat tacacctcg gctatcatt gatgccaac aagacggta aacgaaatt gtttataaa ctagagagg aaaatagac gacattttt tccgcgcta ctgaccact aaagcggca attcagaat ttgcaaatg ctgaattac acactcaaa atacgctct actctcctc ccatgtatc aaaaatcaa acggataag agtgaagaa gcaataata tcattgttg gacaagatt aaaacccat gtgattaca aagaatgac accaaacag cagtatttt atttcaacg tctgatctc cagtgccat tattcgcct tttattaat tgcgagaga attgaccac atgttgttg tggaaaaat ttcatgtct cgaaacaat tatgcttcc aaaaacatt gtcctgttg ctggaccat gggggctcg ctcaacaag cgccacttt gccatagtt ttatcaatt gccaaacaa attgttgaa aatttggac gaagacgat aaaatcgcc atacttgcc atatcaaac gactggtca tctccgctc ttaaatgag gagtgtccg gtgacaaac caagttcct tcagttgtt gacgacgtt ccgaacttt actcacgtt accgccagt aggaagaag ttattgtac aattttatc gacggcatg cacaaaggg agcggtgcc acaaaccac tcgttgggt ctccagaaa gctttaaaa gtaatcgag aacagccag atacaaaac gaaactgtg atgattctg tacataagc cggggttta ctgtcgtcg ttgacggag gccaaagcc gtgcttgag acaatagtg gaggcttcg caaaatatc tcctgtccg tttgttatc aacacgtgt gccgttatt gacgatagt agacccact atttacgaa acgcaattc ctcgaagac attgcgtta caaaattac caaaagtat aacatctcg catcccggg aacgtattc agagggatt atggtgccg attaattcg acggaaacg gtgggcttt gccttggct cgcttttac atggactat aatcaaaac tataacttg caaaaactg atttcgctt ccaacgtgg gatgaaaga tcgaacgac ttgacaatc tccttttcg ttggggtac ttacacaac agtaatttt gggttgttg ggccttgat gtttatttt gccagttgg gccagagaa cttatttat ttcagcaat tcgcacaaa aactactac gcttttttg accgatatg gcaggacgg gttatctta catccgaaa ttcgcatca gttatgtca gtaaaacgg caattacag tacgtcgat atatcatat ttcgaaaac gttgcatat ccacatatt gttaaaaag agacttttg aacgaacgt gaagggttt caattaact actcagttt ggatcacct gtcaaatgg acttggaag cgagtgggt gattggttt atcgtgtgc atagtcaca cacgagtca ccaacacct ctcaagcct caccacatc acgtggcct tttaatacg tcggacgac tacagtatt agacttgtg taccgacat ttattgccg tctgaatca aatttatgt cgccatttc aaccaagct gcaactctg gacgctggt tccctctac ttgagttcg tcgtgcttc caatccgaa aaaaagaca atcgaggag gacctccat ataacaaaa aacaatact cagcaattg gaagatttg ggcttgaag cctgaaatc aaggacgaa gtggcagct cttgcttat attttgagt tttttgcgc agccaacac ttgtctagc ttcaaatcg aagtatata gttcgccgg tatgtggcc tcctacagc ggcgtactt caaatgttt ccgggcagt atcctcgat caaggtcta gatccgacg aagcgagcc tggtttgtg caagctgtt gagcacaag tcgaagatt attatgatt ccgccgtat ttggacgaa ggcggcgct ggttatatt gtgacaatt gcctacgcc acctcggat gtggttgtg gcacttgat gttagttac ggatttgtg ttccgcaca cttttgaaa cgtatgccc ttttgtttg gccacaaat gtcacttgt tttctgatg gatgatcgg gggtacttg atttatcat ccgaatttg ataaatccg aacgagcac gggccggtt gagaagcaa catattgtg cataaagaa tcactggtt gctaatgat attttgagc cataaatat ttcattaag aaggtgcta tgtaacagt tacgggagt ggcaccgtc cagaggtac tacaagcta aatactagt tacagcgat gttttggtt agttcggtc cagggagag cattgcgtc agttaccac atagctgcg atacgaaat agcaacgtt ttcgttggg atggttaac gcgacttgt aacgtcgtg gttacattc tgtccgtgt agtatcgtg gaccggtta tgtctgaac tgcaaccgc atggaacaa aaagaatgt gaatgtccg tgcgagtgt ccgttagaa cagctcaaa tgtacggac tataaccac agtaacaat ccggtttgt aactggttt ccggagcac gtgactctc aaagctcgc tttgttgag gatgccaca agtgagatg aagtcatgc tttccggcc agttgcaag cgcgagaaa actcatttg aggtgtctt ggagtaacc ggttgcgaa tggtgcaag tatgatatt gatggtagt ccactggag agtccgttt tgcgcctcc atggccact tgtttcaat ggaatcatc gggtctgtg acaccctac aggaactcc ctccacgaa attgatctt ccagaagag tccttcagt gtgcccata agtgtcata actttgttc atattcgga gtcctcctc ctcctttgt atgttctac gtgtatcat cgctctctg gcgccgcaa gccaccgaa agattgtac ctttcgtcc actcaggag aatcattta cgaatgagt gatcttaat ttgagtgat aattaccac gctatgggc aaccataga gataagttg cttcacgag gacaagccg gatccgatt tcgccatac tgtgtgtcg agcaattac aagagaacg actttggcg gcggattcg gatcatggc tactcgacg atgactcaa catgacgag tccgagcat atgtctttg gctccggtt gagtttgat tcgttggat gacgatttg gggtcggac agtgtttcc actcatatc agtgtgcca aataaggga catgtcgaa ccagcgcag ttcacatgt attcccaga aacaaatgt attgtggtt ccagtcacg gtccacaga aacatggaa acgacgtaa cagtagata atagcttac ttaataatg cctgtgtat atttctgca ccagtatat ttatttccg tagtgtact taattgtat attttgtaa aatttattt attttaact gtagtttta cccttgtag cttaatttg gtatccatc catcgtgcg catataaag gacttacaa attttttac attacttaa ccgccacac gtccatgtt tgtacaaaa tttgttata tttggactt tgcgataaa ttgtttgag acattctgt gccttagct ccgtttcaa tgtttgttt cctttgcca ctttcgagt aatttgtcg gaaatgtcc cattttgac tgttattga aaaaattgt tacggatta ttgtgcaat acaaacgtt caatcgtag ctatccatt gcataaagt gtacctgtt tttaaggtt tgtgtactt ttttaaaaa taaattctg ttcaattga
