General information | |||
TC034858 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 1610563..1624708 | |
Sequence information | |||
Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
Closest fly homologs |
|
TC034858 |
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
Gene | {{ goGene }} |
|
---|---|---|
GO ID | * {{ blurIdResult }} |
|
Evidence | {{ blurEvidenceResult }} |
|
Reference | * {{ blurReferenceResult }} |
|
Quotation | ||
Name | ||
Publish name | ||
{{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_00316 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (3 dpi)adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)
pupation delayed (still prepupa) - Number of animals showing the phenotype: 8 (11 dpi)
remarks: animals lost, pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 8 (19 dpi)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
General information | ||||
iB_03275 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC034858 RA
MMVKFFVCL LLFFASDVR SMLNGKFKL DNSTDVLTI KHVKNDVVL KSCVGFMIS FTRHWLVSS AFCLHSNPE DKIIVEVGI EDGNNYKVI HYNDTKAVI HPGYDEKTG KHDIGLLFV KETITDKFP YRLKVKFVH FPQMNGFVE SWNMDPATG QKSTRVVYS LFDIYPMSE CKLKFAKFD GIASLNEDF QVCAFPKEN GTGLCEPDS GAPLYVQLD KQRQLYGIV GTFNDIFAV TSGRQHHCD PHHRHPVIF TQLWPYFPW IAEITKYSP LIRLKLLIP VHHANISRC IMFVYSGLV LWNFLAEEL STKVLAVFL MVLFLVRLV QMLKEASAL PPGPWGLPI LGSLPFLKG DLHLHFRDL THKYGSLIS TRLGSQLIV VLSDYKMIR DAFRKEEFT GRPITEFTT LLDGYGVIN TAGKLWKDQ RRFLHDGLR HFGMSYIGS RKTQMENRI MREVEEFLS VLTARKDTP IDLNPVLAV SLSNVICDI LMSVRFSHN DERFKRFMF LIDEGFKLF SSLEASFFI PILKYLPGQ RQTREKIAK NRAEMAQFL QETIEEHRK SFDPSHLRD LLDTYLYEI QKADEEGTG DHLFEGKDH DRQMQQIMG DLFSAGMET IKSSLQWAV LFMLHHPEV MKAVQEELD QVVGRRRLP KLEDLPYLP VTESTMLEV LRISSIVPM GTTHAPTRD LKLNGFHLP RHAQIVPLL HSVHMDPSL WHEPERFNP SRFINAEGK VVKPEYFLP FGVGRRMCL GEILARMEI FSFFSSLLH SFDICVPTG ETLPSLKGV AGVTISPNA FRVCLKPRP MEWDSMGTI RPAGSH
- >TC034858 RA
atgatggtc aagtttttt gtttgtttg ctgcttttc tttgcctct gatgtcagg tcaatgttg aatggaaag tttaaatta gacaatagc accgatgtt ttgaccatc aaacatgta aaaaatgat gtagtgctg aaatcatgt gtgggattt atgatttcc tttacgcga cattggctt gtctcatca gctttttgt ctccatagc aatccggag gataaaata atagtagaa gttggaatc gaagatgga aacaattac aaagtcatt cattataat gacacaaaa gctgtaatt catccaggt tatgacgag aagactgga aaacacgac atcggtttg ctttttgtt aaggaaact attaccgac aaatttcca tacagatta aaagttaaa tttgttcat tttccccaa atgaatgga tttgtagaa agctggaat atggaccca gcaactggc cagaaatca actcgcgtt gtttattca ctctttgat atttatccc atgtctgaa tgcaagttg aaatttgcg aaattcgac ggtattgcg agtttaaat gaagatttt caagtgtgc gcttttcca aaggaaaat ggaacaggt ttatgcgag ccagactct ggtgcgcct ttatatgta caactggat aaacagaga cagctctat ggaatagta gggacattc aacgacatt tttgcagta acaagtgga cgacaacat cattgtgac ccacatcat agacacccg gttattttt acacaactt tggccctat tttccttgg attgccgaa attactaaa tactcccca ctcattcgt cttaagttg ctcatacct gtgcaccac gcgaatatt tcacggtgc atcatgttc gtgtacagt ggcttagtg ttgtggaat ttcctcgcc gaggagctg tccaccaaa gtgttggcc gtcttcctc atggtactg ttcctggta cgtctcgtc cagatgctg aaggaggcc agcgcgctg ccaccgggc ccatggggg ttgcccatc ttgggctcg ctgcccttc ctcaaaggc gacctgcac ctgcacttc agggatctg acccataaa tacggctcg cttatctcg acccggctc gggtcgcaa cttattgtc gtgttgagt gattacaag atgattcgg gacgcgttc aggaaggag gagtttact gggaggccc atcaccgaa tttaccaca cttttggac ggttatggt gtgattaat acggccggg aaattgtgg aaggaccag agacggttc ttacacgat ggtttgcga cactttggg atgtcgtac atcgggagt cgcaaaaca caaatggaa aatcgcatc atgagagag gtcgaagag ttcctctcg gttctgacc gcgcgcaaa gatactccc attgacttg aaccctgtc ttggccgtg tctttatcg aacgtgatt tgtgatatt ttaatgtcg gtcagattc tcgcataat gacgagcgt ttcaaacgc ttcatgttt ctcattgac gaaggattc aaactgttc agttcactg gaagcttcg ttcttcatc cccatcttg aaatattta ccgggacaa cggcaaaca cgcgaaaaa atcgcaaag aaccgtgcc gaaatggcg caattcctc caagaaacc atcgaagag caccgcaaa agcttcgac ccatcccac ctgcgtgac ctgttggac acctacttg tacgagatt cagaaggcg gatgaggaa ggcaccggt gatcacctc ttcgagggc aaagaccac gaccgtcag atgcagcaa atcatgggc gatttgttc tccgccggg atggaaacc atcaaaagt tcgctgcag tgggccgtc ctcttcatg ctgcatcac cccgaagtg atgaaggca gtgcaagag gagttggac caagtggtt ggacgtcgt cggctcccc aagttggag gatttgccc tatttgcct gtgacggag tcgacgatg ttggaagtg ttgcggatt tccagcatt gttccaatg ggcacgaca cacgcaccc acaagggat ttgaaactg aacgggttc catctgcca cggcatgcc cagatcgtg ccccttctg cactctgtc cacatggac cccagtctg tggcacgag ccggagcgc ttcaacccg agccggttc atcaacgct gaaggcaaa gtggtcaag cctgagtac tttctgccc ttcggggtg gggaggagg atgtgtttg ggggagatc ttggcccgc atggagatc ttctccttc ttctcctcg ctgctgcac tcgttcgac atctgcgtg cccacgggg gagaccctc cccagcttg aagggcgtg gcgggggtg accatcagt cccaacgcg ttccgggtg tgtctcaag cccagaccc atggagtgg gactcgatg ggcaccatc agaccagcg ggcagccac tag
- >TC034858 RA
tttaaaatc tcgcaatga tggtcaagt tttttgttt gtttgctgc ttttctttg cctctgatg tcaggtcaa tgttgaatg gaaagttta aattagaca atagcaccg atgttttga ccatcaaac atgtaaaaa atgatgtag tgctgaaat catgtgtgg gatttatga tttccttta cgcgacatt ggcttgtct catcagctt tttgtctcc atagcaatc cggaggata aaataatag tagaagttg gaatcgaag atggaaaca attacaaag tcattcatt ataatgaca caaaagctg taattcatc caggttatg acgagaaga ctggaaaac acgacatcg gtttgcttt ttgttaagg aaactatta ccgacaaat ttccataca gattaaaag ttaaatttg ttcattttc cccaaatga atggatttg tagaaagct ggaatatgg acccagcaa ctggccaga aatcaactc gcgttgttt attcactct ttgatattt atcccatgt ctgaatgca agttgaaat ttgcgaaat tcgacggta ttgcgagtt taaatgaag attttcaag tgtgcgctt ttccaaagg aaaatggaa caggtttat gcgagccag actctggtg cgcctttat atgtacaac tggataaac agagacagc tctatggaa tagtaggga cattcaacg acatttttg cagtaacaa gtggacgac aacatcatt gtgacccac atcatagac acccggtta tttttacac aactttggc cctattttc cttggattg ccgaaatta ctaaatact ccccactca ttcgtctta agttgctca tacctgtgc accacgcga atatttcac ggtgcatca tgttcgtgt acagtggct tagtgttgt ggaatttcc tcgccgagg agctgtcca ccaaagtgt tggccgtct tcctcatgg tactgttcc tggtacgtc tcgtccaga tgctgaagg aggccagcg cgctgccac cgggcccat gggggttgc ccatcttgg gctcgctgc ccttcctca aaggcgacc tgcacctgc acttcaggg atctgaccc ataaatacg gctcgctta tctcgaccc ggctcgggt cgcaactta ttgtcgtgt tgagtgatt acaagatga ttcgggacg cgttcagga aggaggagt ttactggga ggcccatca ccgaattta ccacacttt tggacggtt atggtgtga ttaatacgg ccgggaaat tgtggaagg accagagac ggttcttac acgatggtt tgcgacact ttgggatgt cgtacatcg ggagtcgca aaacacaaa tggaaaatc gcatcatga gagaggtcg aagagttcc tctcggttc tgaccgcgc gcaaagata ctcccattg acttgaacc ctgtcttgg ccgtgtctt tatcgaacg tgatttgtg atattttaa tgtcggtca gattctcgc ataatgacg agcgtttca aacgcttca tgtttctca ttgacgaag gattcaaac tgttcagtt cactggaag cttcgttct tcatcccca tcttgaaat atttaccgg gacaacggc aaacacgcg aaaaaatcg caaagaacc gtgccgaaa tggcgcaat tcctccaag aaaccatcg aagagcacc gcaaaagct tcgacccat cccacctgc gtgacctgt tggacacct acttgtacg agattcaga aggcggatg aggaaggca ccggtgatc acctcttcg agggcaaag accacgacc gtcagatgc agcaaatca tgggcgatt tgttctccg ccgggatgg aaaccatca aaagttcgc tgcagtggg ccgtcctct tcatgctgc atcaccccg aagtgatga aggcagtgc aagaggagt tggaccaag tggttggac gtcgtcggc tccccaagt tggaggatt tgccctatt tgcctgtga cggagtcga cgatgttgg aagtgttgc ggatttcca gcattgttc caatgggca cgacacacg cacccacaa gggatttga aactgaacg ggttccatc tgccacggc atgcccaga tcgtgcccc ttctgcact ctgtccaca tggacccca gtctgtggc acgagccgg agcgcttca acccgagcc ggttcatca acgctgaag gcaaagtgg tcaagcctg agtactttc tgcccttcg gggtgggga ggaggatgt gtttggggg agatcttgg cccgcatgg agatcttct ccttcttct cctcgctgc tgcactcgt tcgacatct gcgtgccca cgggggaga ccctcccca gcttgaagg gcgtggcgg gggtgacca tcagtccca acgcgttcc gggtgtgtc tcaagccca gacccatgg agtgggact cgatgggca ccatcagac cagcgggca gccactagt gggtcagga gcacggtga ccgaatgcg tttcctata tttatatat cgaggcaca gtacttaga cattcctca gttagataa gagatctcc gacttgttc ggctgtgca ttgcaagtg ttcattcat gttagtatt aatttttaa gtgctagtc acgtcatcg tccagttgc cgaaaagta ccattgtat cacgttacc tgctgtaaa ttggcaact ttcacacca ttttatcgt tctaggtta agccacttg taaatatca ccctatttt attttttga tcttgctaa ctgtacaag atgataatt gtgtaatta at
If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.
