Status
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Gene information
General information
TC034858 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 1610563..1624708
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC034858
    iBeetle screen iB_00316

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 90.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 7 (3 dpi)
    Days post injection

    adult lethality immediately after eclosion - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 20 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

    affected eggs/embryos/larvae total percentage of affected eggs/embryos/larvae on slide - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: 20.0% (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: 100.0% (includes death as larva, prepupa, pupa, adult)

    larva lethality early (L5/6) - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    pupation delayed (still prepupa) - Number of animals showing the phenotype: 8 (11 dpi)
    Days post injection

    remarks: animals lost, pupal molt not fulfilled (died as prepupa) - Number of animals showing the phenotype: 8 (19 dpi)
    Days post injection

    Pupal morphology
    remarks: animals lost,
    iBeetle screen iB_03275

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC034858 RA
      MMVKFFVCL LLFFASDVR SMLNGKFKL DNSTDVLTI 
KHVKNDVVL KSCVGFMIS FTRHWLVSS AFCLHSNPE 
DKIIVEVGI EDGNNYKVI HYNDTKAVI HPGYDEKTG 
KHDIGLLFV KETITDKFP YRLKVKFVH FPQMNGFVE 
SWNMDPATG QKSTRVVYS LFDIYPMSE CKLKFAKFD 
GIASLNEDF QVCAFPKEN GTGLCEPDS GAPLYVQLD 
KQRQLYGIV GTFNDIFAV TSGRQHHCD PHHRHPVIF 
TQLWPYFPW IAEITKYSP LIRLKLLIP VHHANISRC 
IMFVYSGLV LWNFLAEEL STKVLAVFL MVLFLVRLV 
QMLKEASAL PPGPWGLPI LGSLPFLKG DLHLHFRDL 
THKYGSLIS TRLGSQLIV VLSDYKMIR DAFRKEEFT 
GRPITEFTT LLDGYGVIN TAGKLWKDQ RRFLHDGLR 
HFGMSYIGS RKTQMENRI MREVEEFLS VLTARKDTP 
IDLNPVLAV SLSNVICDI LMSVRFSHN DERFKRFMF 
LIDEGFKLF SSLEASFFI PILKYLPGQ RQTREKIAK 
NRAEMAQFL QETIEEHRK SFDPSHLRD LLDTYLYEI 
QKADEEGTG DHLFEGKDH DRQMQQIMG DLFSAGMET 
IKSSLQWAV LFMLHHPEV MKAVQEELD QVVGRRRLP 
KLEDLPYLP VTESTMLEV LRISSIVPM GTTHAPTRD 
LKLNGFHLP RHAQIVPLL HSVHMDPSL WHEPERFNP 
SRFINAEGK VVKPEYFLP FGVGRRMCL GEILARMEI 
FSFFSSLLH SFDICVPTG ETLPSLKGV AGVTISPNA 
FRVCLKPRP MEWDSMGTI RPAGSH
    CDS sequence
    • >TC034858 RA
      atgatggtc aagtttttt gtttgtttg ctgcttttc 
tttgcctct gatgtcagg tcaatgttg aatggaaag 
tttaaatta gacaatagc accgatgtt ttgaccatc 
aaacatgta aaaaatgat gtagtgctg aaatcatgt 
gtgggattt atgatttcc tttacgcga cattggctt 
gtctcatca gctttttgt ctccatagc aatccggag 
gataaaata atagtagaa gttggaatc gaagatgga 
aacaattac aaagtcatt cattataat gacacaaaa 
gctgtaatt catccaggt tatgacgag aagactgga 
aaacacgac atcggtttg ctttttgtt aaggaaact 
attaccgac aaatttcca tacagatta aaagttaaa 
tttgttcat tttccccaa atgaatgga tttgtagaa 
agctggaat atggaccca gcaactggc cagaaatca 
actcgcgtt gtttattca ctctttgat atttatccc 
atgtctgaa tgcaagttg aaatttgcg aaattcgac 
ggtattgcg agtttaaat gaagatttt caagtgtgc 
gcttttcca aaggaaaat ggaacaggt ttatgcgag 
ccagactct ggtgcgcct ttatatgta caactggat 
aaacagaga cagctctat ggaatagta gggacattc 
aacgacatt tttgcagta acaagtgga cgacaacat 
cattgtgac ccacatcat agacacccg gttattttt 
acacaactt tggccctat tttccttgg attgccgaa 
attactaaa tactcccca ctcattcgt cttaagttg 
ctcatacct gtgcaccac gcgaatatt tcacggtgc 
atcatgttc gtgtacagt ggcttagtg ttgtggaat 
ttcctcgcc gaggagctg tccaccaaa gtgttggcc 
gtcttcctc atggtactg ttcctggta cgtctcgtc 
cagatgctg aaggaggcc agcgcgctg ccaccgggc 
ccatggggg ttgcccatc ttgggctcg ctgcccttc 
ctcaaaggc gacctgcac ctgcacttc agggatctg 
acccataaa tacggctcg cttatctcg acccggctc 
gggtcgcaa cttattgtc gtgttgagt gattacaag 
atgattcgg gacgcgttc aggaaggag gagtttact 
gggaggccc atcaccgaa tttaccaca cttttggac 
ggttatggt gtgattaat acggccggg aaattgtgg 
aaggaccag agacggttc ttacacgat ggtttgcga 
cactttggg atgtcgtac atcgggagt cgcaaaaca 
caaatggaa aatcgcatc atgagagag gtcgaagag 
ttcctctcg gttctgacc gcgcgcaaa gatactccc 
attgacttg aaccctgtc ttggccgtg tctttatcg 
aacgtgatt tgtgatatt ttaatgtcg gtcagattc 
tcgcataat gacgagcgt ttcaaacgc ttcatgttt 
ctcattgac gaaggattc aaactgttc agttcactg 
gaagcttcg ttcttcatc cccatcttg aaatattta 
ccgggacaa cggcaaaca cgcgaaaaa atcgcaaag 
aaccgtgcc gaaatggcg caattcctc caagaaacc 
atcgaagag caccgcaaa agcttcgac ccatcccac 
ctgcgtgac ctgttggac acctacttg tacgagatt 
cagaaggcg gatgaggaa ggcaccggt gatcacctc 
ttcgagggc aaagaccac gaccgtcag atgcagcaa 
atcatgggc gatttgttc tccgccggg atggaaacc 
atcaaaagt tcgctgcag tgggccgtc ctcttcatg 
ctgcatcac cccgaagtg atgaaggca gtgcaagag 
gagttggac caagtggtt ggacgtcgt cggctcccc 
aagttggag gatttgccc tatttgcct gtgacggag 
tcgacgatg ttggaagtg ttgcggatt tccagcatt 
gttccaatg ggcacgaca cacgcaccc acaagggat 
ttgaaactg aacgggttc catctgcca cggcatgcc 
cagatcgtg ccccttctg cactctgtc cacatggac 
cccagtctg tggcacgag ccggagcgc ttcaacccg 
agccggttc atcaacgct gaaggcaaa gtggtcaag 
cctgagtac tttctgccc ttcggggtg gggaggagg 
atgtgtttg ggggagatc ttggcccgc atggagatc 
ttctccttc ttctcctcg ctgctgcac tcgttcgac 
atctgcgtg cccacgggg gagaccctc cccagcttg 
aagggcgtg gcgggggtg accatcagt cccaacgcg 
ttccgggtg tgtctcaag cccagaccc atggagtgg 
gactcgatg ggcaccatc agaccagcg ggcagccac 
tag
    mRNA sequence
    • >TC034858 RA
      tttaaaatc tcgcaatga tggtcaagt tttttgttt 
gtttgctgc ttttctttg cctctgatg tcaggtcaa 
tgttgaatg gaaagttta aattagaca atagcaccg 
atgttttga ccatcaaac atgtaaaaa atgatgtag 
tgctgaaat catgtgtgg gatttatga tttccttta 
cgcgacatt ggcttgtct catcagctt tttgtctcc 
atagcaatc cggaggata aaataatag tagaagttg 
gaatcgaag atggaaaca attacaaag tcattcatt 
ataatgaca caaaagctg taattcatc caggttatg 
acgagaaga ctggaaaac acgacatcg gtttgcttt 
ttgttaagg aaactatta ccgacaaat ttccataca 
gattaaaag ttaaatttg ttcattttc cccaaatga 
atggatttg tagaaagct ggaatatgg acccagcaa 
ctggccaga aatcaactc gcgttgttt attcactct 
ttgatattt atcccatgt ctgaatgca agttgaaat 
ttgcgaaat tcgacggta ttgcgagtt taaatgaag 
attttcaag tgtgcgctt ttccaaagg aaaatggaa 
caggtttat gcgagccag actctggtg cgcctttat 
atgtacaac tggataaac agagacagc tctatggaa 
tagtaggga cattcaacg acatttttg cagtaacaa 
gtggacgac aacatcatt gtgacccac atcatagac 
acccggtta tttttacac aactttggc cctattttc 
cttggattg ccgaaatta ctaaatact ccccactca 
ttcgtctta agttgctca tacctgtgc accacgcga 
atatttcac ggtgcatca tgttcgtgt acagtggct 
tagtgttgt ggaatttcc tcgccgagg agctgtcca 
ccaaagtgt tggccgtct tcctcatgg tactgttcc 
tggtacgtc tcgtccaga tgctgaagg aggccagcg 
cgctgccac cgggcccat gggggttgc ccatcttgg 
gctcgctgc ccttcctca aaggcgacc tgcacctgc 
acttcaggg atctgaccc ataaatacg gctcgctta 
tctcgaccc ggctcgggt cgcaactta ttgtcgtgt 
tgagtgatt acaagatga ttcgggacg cgttcagga 
aggaggagt ttactggga ggcccatca ccgaattta 
ccacacttt tggacggtt atggtgtga ttaatacgg 
ccgggaaat tgtggaagg accagagac ggttcttac 
acgatggtt tgcgacact ttgggatgt cgtacatcg 
ggagtcgca aaacacaaa tggaaaatc gcatcatga 
gagaggtcg aagagttcc tctcggttc tgaccgcgc 
gcaaagata ctcccattg acttgaacc ctgtcttgg 
ccgtgtctt tatcgaacg tgatttgtg atattttaa 
tgtcggtca gattctcgc ataatgacg agcgtttca 
aacgcttca tgtttctca ttgacgaag gattcaaac 
tgttcagtt cactggaag cttcgttct tcatcccca 
tcttgaaat atttaccgg gacaacggc aaacacgcg 
aaaaaatcg caaagaacc gtgccgaaa tggcgcaat 
tcctccaag aaaccatcg aagagcacc gcaaaagct 
tcgacccat cccacctgc gtgacctgt tggacacct 
acttgtacg agattcaga aggcggatg aggaaggca 
ccggtgatc acctcttcg agggcaaag accacgacc 
gtcagatgc agcaaatca tgggcgatt tgttctccg 
ccgggatgg aaaccatca aaagttcgc tgcagtggg 
ccgtcctct tcatgctgc atcaccccg aagtgatga 
aggcagtgc aagaggagt tggaccaag tggttggac 
gtcgtcggc tccccaagt tggaggatt tgccctatt 
tgcctgtga cggagtcga cgatgttgg aagtgttgc 
ggatttcca gcattgttc caatgggca cgacacacg 
cacccacaa gggatttga aactgaacg ggttccatc 
tgccacggc atgcccaga tcgtgcccc ttctgcact 
ctgtccaca tggacccca gtctgtggc acgagccgg 
agcgcttca acccgagcc ggttcatca acgctgaag 
gcaaagtgg tcaagcctg agtactttc tgcccttcg 
gggtgggga ggaggatgt gtttggggg agatcttgg 
cccgcatgg agatcttct ccttcttct cctcgctgc 
tgcactcgt tcgacatct gcgtgccca cgggggaga 
ccctcccca gcttgaagg gcgtggcgg gggtgacca 
tcagtccca acgcgttcc gggtgtgtc tcaagccca 
gacccatgg agtgggact cgatgggca ccatcagac 
cagcgggca gccactagt gggtcagga gcacggtga 
ccgaatgcg tttcctata tttatatat cgaggcaca 
gtacttaga cattcctca gttagataa gagatctcc 
gacttgttc ggctgtgca ttgcaagtg ttcattcat 
gttagtatt aatttttaa gtgctagtc acgtcatcg 
tccagttgc cgaaaagta ccattgtat cacgttacc 
tgctgtaaa ttggcaact ttcacacca ttttatcgt 
tctaggtta agccacttg taaatatca ccctatttt 
attttttga tcttgctaa ctgtacaag atgataatt 
gtgtaatta at
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY