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Gene information
General information
TC001920 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid63: 315446..332377
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001920
    iBeetle screen iB_03269
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001920 RA
      MSKKFKLSA SLFGHSLDV RSVVVTNNN SIISGSRDK 
      TAKFWTPNR IDTGYTPIQ TFRDQKNFV IAVLYLEPT 
      SEYPDGLVI TGGNDNTIY VYKPSEPFA TFTLKEHTN 
      AVSCFSKYS TSNNNAFLS GSWDCSAKL WTLGNSTST 
      VTFSGHLAA IWSIIQLAD SRIVTASAD KTIGIWSSE 
      GARLNSLTG HTDCVRGLV DLSELHQFV SVANDATIR 
      VWSYAGESQ GVLYGHTNY IYSIARCKS AGENCFVTS 
      DEDRTVRFW QNGENIETI QLPAQSVWS VACLSNGDI 
      VTGSSDGVV RVFTQNESR YADEATLNK FNEEVEALT 
      RQSTQEIGG YKISDLPGK EALYDPGRK AGQMKMIRE 
      GTGVVAYTW VEDGDKSHW EKVGDVLGS TDKTNQDKT 
      MYEGKAYDF VFSVDVEDG KPPLKLPYN KGDDPYQAA 
      HNFLAKNFL PASYLEQVV DFILKNTQE KYVPPSTEY 
      VDPFTGGSR YTPSTGNNS QDFAGRNFD PFTGGSSYS 
      TSATQAKQT VSSSTISSQ VSKFFPINT YRTFEMGDS 
      NVILVKLKE FNEKTGDSQ SPPVNEHYL VELVKLCNG 
      PPDDPNAFD TLFKLLEWP DEIVFPVVD VIRMAVRFK 
      KNNEIIATA NSGSLLRKL LSFINENCN IINNVIVAL 
      RTLSNLLMH EFGEDLVFE HRFDVVENI TALGPLNKN 
      GQIALSTLL LNLCVASLK KRDDLGISV LADVIPDIL 
      TKLSDPESQ FRSYVALGT LLSSPQSAE VKAKVKSNV 
      GFISALESH VLSGQGDLE EKRRNCASQ VQEILNLSF
    CDS sequence
    • >TC001920 RA
      atgtccaaa aaattcaag ttaagtgct tcacttttt 
      ggacattcg ttagatgta agaagtgta gtcgtgaca 
      aacaataat tctataatt agcggatct cgtgataaa 
      actgccaag ttttggacg cccaacagg atcgacact 
      ggatataca cctattcag acttttcga gaccagaag 
      aactttgtt attgccgtt ttgtacttg gaaccgact 
      agtgaatat ccagacggt ttggttata acagggggc 
      aatgacaac acgatttat gtctacaaa ccctcagag 
      ccttttgct acgtttacg ttgaaggaa cacacaaat 
      gcagtgagt tgtttttcg aagtattcg acttcgaat 
      aacaatgca tttttgagc ggatcgtgg gattgttcg 
      gccaaatta tggacattg ggtaattcc acgtcaacg 
      gtgactttc tcaggccat ttggctgca atttggtca 
      atcattcaa ttagccgat tcacgaatt gtaaccgct 
      tcagctgat aaaaccatt ggaatttgg agtagtgaa 
      ggtgcaagg ttgaattcg ctcactggt cacacggat 
      tgtgtcaga ggtttggtg gatttatcc gaattgcat 
      cagttcgtt tcggtcgcg aacgatgcc accatcagg 
      gtttggtca tatgctgga gaaagtcaa ggagtgctt 
      tatggacac accaattat atttacagt attgcgcgt 
      tgcaaatca gccggagag aattgtttc gtgacatcg 
      gatgaagac cgaactgtt cgattttgg cagaacgga 
      gaaaatatt gaaacgatt caattaccg gcacaatcc 
      gtttggtca gtcgcctgt ttatcaaat ggcgacatt 
      gtgacaggt tctagcgat ggagttgtt cgtgttttc 
      actcaaaat gaaagcaga tatgccgat gaagccact 
      ttgaacaaa ttcaatgaa gaagtggaa gctttgacg 
      cgacaaagc actcaagaa atcggcggt tataaaatc 
      tcagatttg ccaggtaag gaagcgtta tacgatccc 
      ggaaggaag gctgggcaa atgaaaatg atcagagaa 
      ggcacagga gtggtggca tacacatgg gtagaagat 
      ggggacaag agtcattgg gaaaaagtt ggcgatgtc 
      ctcggaagt acggacaaa accaatcaa gacaaaaca 
      atgtacgaa ggaaaagcg tacgatttt gtgttttcc 
      gttgatgtg gaagatgga aaacctccg ttgaaattg 
      ccgtataat aaaggagat gatccgtat caagctgcg 
      cataatttt ttagcgaag aattttttg ccggcttcg 
      tatttggaa caagttgtt gattttatt ttgaaaaat 
      actcaggaa aaatacgtt ccacctagt acggagtat 
      gtcgatccg tttacgggt ggttcaaga tatacacca 
      agtactgga aataacagt caggatttt gcaggaagg 
      aattttgat cctttcaca ggtggcagc agctattca 
      acatcggca acccaagca aaacaaacc gttagctca 
      tccactatt tcaagccaa gttagcaaa ttcttccct 
      attaacacc taccgcacc ttcgaaatg ggcgattcc 
      aacgtaatt ttagtcaaa ttaaaagaa ttcaacgaa 
      aaaaccggc gatagtcaa agtcctcca gtaaacgaa 
      cattactta gtcgaattg gtgaaatta tgtaatgga 
      ccaccggat gatcccaac gctttcgac accttattc 
      aaactgttg gaatggcct gatgaaatc gtttttccg 
      gtcgttgat gtcataaga atggccgta aggttcaag 
      aaaaacaac gaaattatt gccactgcc aatagtgga 
      agtctttta cgaaaattg ctcagtttc atcaatgag 
      aattgtaat ataattaat aacgtaatt gttgcattg 
      cgtactttg agcaattta ttgatgcac gaatttggc 
      gaagacttg gtttttgaa caccgattt gatgttgtg 
      gaaaatatc acagctttg ggacctttg aataaaaac 
      ggacagatc gctttatcc actcttctc ctaaatctt 
      tgcgttgca agtctgaag aaacgagac gatttagga 
      atttcagtc ctagccgac gtgattcca gacatttta 
      acgaaactg agcgaccct gagtcgcaa tttcgcagt 
      tacgttgct ttagggaca cttctttcc tcacctcaa 
      agtgctgaa gtcaaagcc aaagttaag agcaacgtt 
      ggattcatc agtgcctta gaatcgcat gtgttatca 
      ggacaaggt gatttggag gagaagaga cggaattgt 
      gccagtcaa gtacaggag attcttaat ttaagtttt 
      taa
    mRNA sequence
    • >TC001920 RA
      acgtaacca aacttgctc gtcatagca gtgaaaata 
      tcgtcaaaa atgtccaaa aaattcaag ttaagtgct 
      tcacttttt ggacattcg ttagatgta agaagtgta 
      gtcgtgaca aacaataat tctataatt agcggatct 
      cgtgataaa actgccaag ttttggacg cccaacagg 
      atcgacact ggatataca cctattcag acttttcga 
      gaccagaag aactttgtt attgccgtt ttgtacttg 
      gaaccgact agtgaatat ccagacggt ttggttata 
      acagggggc aatgacaac acgatttat gtctacaaa 
      ccctcagag ccttttgct acgtttacg ttgaaggaa 
      cacacaaat gcagtgagt tgtttttcg aagtattcg 
      acttcgaat aacaatgca tttttgagc ggatcgtgg 
      gattgttcg gccaaatta tggacattg ggtaattcc 
      acgtcaacg gtgactttc tcaggccat ttggctgca 
      atttggtca atcattcaa ttagccgat tcacgaatt 
      gtaaccgct tcagctgat aaaaccatt ggaatttgg 
      agtagtgaa ggtgcaagg ttgaattcg ctcactggt 
      cacacggat tgtgtcaga ggtttggtg gatttatcc 
      gaattgcat cagttcgtt tcggtcgcg aacgatgcc 
      accatcagg gtttggtca tatgctgga gaaagtcaa 
      ggagtgctt tatggacac accaattat atttacagt 
      attgcgcgt tgcaaatca gccggagag aattgtttc 
      gtgacatcg gatgaagac cgaactgtt cgattttgg 
      cagaacgga gaaaatatt gaaacgatt caattaccg 
      gcacaatcc gtttggtca gtcgcctgt ttatcaaat 
      ggcgacatt gtgacaggt tctagcgat ggagttgtt 
      cgtgttttc actcaaaat gaaagcaga tatgccgat 
      gaagccact ttgaacaaa ttcaatgaa gaagtggaa 
      gctttgacg cgacaaagc actcaagaa atcggcggt 
      tataaaatc tcagatttg ccaggtaag gaagcgtta 
      tacgatccc ggaaggaag gctgggcaa atgaaaatg 
      atcagagaa ggcacagga gtggtggca tacacatgg 
      gtagaagat ggggacaag agtcattgg gaaaaagtt 
      ggcgatgtc ctcggaagt acggacaaa accaatcaa 
      gacaaaaca atgtacgaa ggaaaagcg tacgatttt 
      gtgttttcc gttgatgtg gaagatgga aaacctccg 
      ttgaaattg ccgtataat aaaggagat gatccgtat 
      caagctgcg cataatttt ttagcgaag aattttttg 
      ccggcttcg tatttggaa caagttgtt gattttatt 
      ttgaaaaat actcaggaa aaatacgtt ccacctagt 
      acggagtat gtcgatccg tttacgggt ggttcaaga 
      tatacacca agtactgga aataacagt caggatttt 
      gcaggaagg aattttgat cctttcaca ggtggcagc 
      agctattca acatcggca acccaagca aaacaaacc 
      gttagctca tccactatt tcaagccaa gttagcaaa 
      ttcttccct attaacacc taccgcacc ttcgaaatg 
      ggcgattcc aacgtaatt ttagtcaaa ttaaaagaa 
      ttcaacgaa aaaaccggc gatagtcaa agtcctcca 
      gtaaacgaa cattactta gtcgaattg gtgaaatta 
      tgtaatgga ccaccggat gatcccaac gctttcgac 
      accttattc aaactgttg gaatggcct gatgaaatc 
      gtttttccg gtcgttgat gtcataaga atggccgta 
      aggttcaag aaaaacaac gaaattatt gccactgcc 
      aatagtgga agtctttta cgaaaattg ctcagtttc 
      atcaatgag aattgtaat ataattaat aacgtaatt 
      gttgcattg cgtactttg agcaattta ttgatgcac 
      gaatttggc gaagacttg gtttttgaa caccgattt 
      gatgttgtg gaaaatatc acagctttg ggacctttg 
      aataaaaac ggacagatc gctttatcc actcttctc 
      ctaaatctt tgcgttgca agtctgaag aaacgagac 
      gatttagga atttcagtc ctagccgac gtgattcca 
      gacatttta acgaaactg agcgaccct gagtcgcaa 
      tttcgcagt tacgttgct ttagggaca cttctttcc 
      tcacctcaa agtgctgaa gtcaaagcc aaagttaag 
      agcaacgtt ggattcatc agtgcctta gaatcgcat 
      gtgttatca ggacaaggt gatttggag gagaagaga 
      cggaattgt gccagtcaa gtacaggag attcttaat 
      ttaagtttt taatggttt ttatttaat aaattccaa 
      cgtgtttg