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Gene information
General information
TC001918 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid63: 335920..348457
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001918
    iBeetle screen iB_03268
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001918 RA
      MTQEELWSY LGREIVNSD LGHDKIKFL GFLSCTRYC 
      GEKYRPNFT HDEIVKITQ AYVALGGGG LALFGSACL 
      HTWPENISQ VIPNFINQK PIDRAKFMD DSCYRGTLG 
      ACFSTTLGS VLHELCHTF DLGHTEKGI MGRGFDDIY 
      KVFVCNKRS TEMKKFDED GTFWTRSCL VLLAYHKWL 
      NNHPNDGKG LLKFDPVHK ILSSTKGLR VIELRNEGN 
      GMVLFNWVF EGRILKYSF QIPNDQLVT ENYVMIVED 
      NVGNILKHE IKLIV
    CDS sequence
    • >TC001918 RA
      atgacacaa gaagaattg tggagttat ttaggacgt 
      gaaattgtc aattcggat ttaggacac gacaaaatc 
      aagtttttg ggatttttg tcttgcacg agatactgt 
      ggcgaaaaa tatcgtcca aatttcact catgacgaa 
      atcgtcaaa ataacccag gcttacgtt gctttgggc 
      ggtggaggt ttggcactt ttcggttcg gcttgtctc 
      cacacatgg cccgaaaac atctcccaa gtcatccca 
      aactttatc aatcaaaaa ccaatcgat cgagcaaaa 
      ttcatggat gatagttgc taccgtggt actttgggc 
      gcttgcttt tcgacaact ttgggctca gttttacac 
      gaattgtgc cacactttt gatttaggg cacacggaa 
      aagggcatc atgggacga gggttcgac gatatttac 
      aaagttttc gtatgtaat aaaagaagc accgaaatg 
      aagaaattt gacgaagat ggaacgttt tggacacgg 
      agttgtttg gtgcttttg gcttatcac aaatggcta 
      aataatcac ccaaacgac ggaaaaggc ttattaaaa 
      tttgaccca gtccacaaa attctaagc tcgactaaa 
      ggtcttcga gtaatcgaa ttacgaaat gaaggcaac 
      ggaatggtt ttattcaat tgggtgttt gagggacgt 
      atcctgaaa tattccttc caaattccc aacgaccag 
      ctagtgact gaaaattat gttatgatt gtcgaagat 
      aacgttgga aatatttta aaacatgaa ataaaatta 
      atcgtgtag
    mRNA sequence
    • >TC001918 RA
      cttcacaaa tttgtaaag taaggtgaa aatagtgtt 
      gtcaaagaa ctgatgttt cgtgttgtt tgaagaaaa 
      ccttaggat gtattgtta ctgttatca aaattaatt 
      gagtgataa gtgaggtta cctggctaa aaattcgat 
      ttagtgcag gtggcgacc tctagcgta cgaattgtt 
      aagtattac agaaatctg catcaagga aataatgtc 
      atgcgctca tgaaataac aatcaataa ttttaaaac 
      cgaagaaac tgtaaatta tcccgttgt aatagttaa 
      aggaacagt tgaaaataa caagtgtaa aaaactaca 
      aatcgatga aattgagat taaaattag ttctacaaa 
      atttaattc caaaatagt tgataacaa atttaaatt 
      tttagtaga attaaacct tggtcaaaa caccataac 
      tgtgaatta ttgtaattc aggtgttac tttaaattt 
      gaattatgt tcagaagga gtcggtttt tgcggtact 
      tcccctata cataatttg tgaccaaca cgatggaac 
      atttcaagc accagaaac tatacctaa cgatagaaa 
      cagtgcttg taaaagaat taatttggg tgttaagct 
      gttacaaag tgttattgc cgaaaaact gtacgaagg 
      aaatttcgg tagaaaaac tttcaaatc actcgattg 
      taaaatttt tcatagtcg tttgaattt tcgtgatgc 
      ttggaaaat gacacaaga agaattgtg gagttattt 
      aggacgtga aattgtcaa ttcggattt aggacacga 
      caaaatcaa gtttttggg atttttgtc ttgcacgag 
      atactgtgg cgaaaaata tcgtccaaa tttcactca 
      tgacgaaat cgtcaaaat aacccaggc ttacgttgc 
      tttgggcgg tggaggttt ggcactttt cggttcggc 
      ttgtctcca cacatggcc cgaaaacat ctcccaagt 
      catcccaaa ctttatcaa tcaaaaacc aatcgatcg 
      agcaaaatt catggatga tagttgcta ccgtggtac 
      tttgggcgc ttgcttttc gacaacttt gggctcagt 
      tttacacga attgtgcca cacttttga tttagggca 
      cacggaaaa gggcatcat gggacgagg gttcgacga 
      tatttacaa agttttcgt atgtaataa aagaagcac 
      cgaaatgaa gaaatttga cgaagatgg aacgttttg 
      gacacggag ttgtttggt gcttttggc ttatcacaa 
      atggctaaa taatcaccc aaacgacgg aaaaggctt 
      attaaaatt tgacccagt ccacaaaat tctaagctc 
      gactaaagg tcttcgagt aatcgaatt acgaaatga 
      aggcaacgg aatggtttt attcaattg ggtgtttga 
      gggacgtat cctgaaata ttccttcca aattcccaa 
      cgaccagct agtgactga aaattatgt tatgattgt 
      cgaagataa cgttggaaa tattttaaa acatgaaat 
      aaaattaat cgtgtagtt ggtaattat tccctgatt 
      acataaccc ctattagcg tcttcgatg atgtttg