Status
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Gene information
General information
TC031771
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001880,TC001879
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 285447..293651
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031771
    iBeetle screen iB_03265

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031771 RA
      MEDALSIDS KLIKMGATG NPNITRLAT LQERKKTIE 
      ESLAKCNQE LKQICLQEA DLTGIIPTE MPLEPGESP 
      PLIRRRVGT AYQLPENLI KSSKDESLK DLELQIQVH 
      ANMAEAALG LANEQNISK TLRRQHMAE YQQHKIQFT 
      ALQEKLSAL KDKAQQNEL HKQKKKPRP AEQDDNVSL 
      GTNMNEPFG KSDLRHSMR SNKCDTDVE QRYLLSNPR 
      MTYKNVTNS YMEGSFTRQ EESIARGLY ALSFNGYKN 
      YIERQESLA NNHIGGYST LQYSQLQHS PVNSHYSQS 
      YQPNPRPNT LYNYPVNQN FQVPQVIQH HSPQSTRSQ 
      HRQSSPNLN TTKMFYAQN PDYRLHSEN PHQIQPHQQ 
      YEHMTLGLG GYWKRLDSG QTVWCNSSP TESNWQRDK 
      RFGSLDRRK NKRLHKRVS PSVSSKSAT LATVPVYAE 
      QMRTPSIKS PQVISRRSQ DNRQLVRTQ SLGSVGGQT 
      VDSVWPSDD NSSCESDNR SINEAGQNA RKQKQKEWM 
      ETFLDGPVS PTHSVISRS QSALPSVLS PEEKYIVSP 
      PQPIPPPQT PKPPLEIPA ESNPSPRIP ETNIELFNN 
      NIPKNCTIV QAGHYKPYH EETKPFEMS DYYKYSAKF 
      KKSPQKQQE DTLKKSQGG ASFQKNLNE AFDKPVKNH 
      NFSPGSPRL DNSVNSSFD LSQNVAERF SDEMNAWYF 
      VNCCIYFIP NGWSKMGKE KTHINIVVI GHVDSGKST 
      TTGHLIYKC GGIDKRTIE KFEKEAQEM GKGSFKYAW 
      VLDKLKAER ERGITIDIA LWKFETSKY YVTIIDAPG 
      HRDFIKNMI TGTSQADCA VLIVAAGTG EFEAGISKN 
      GQTREHALL AFTLGVKQL IVGVNKMDS TEPPYSEAR 
      YEEIKKEVS SYIKKIGYN PAAVAFVPI SGWHGDNML 
      EPSTKMPWF KGWNIERKE GKAEGKCLI EALDAILPP 
      TRPTDKPLR LPLQDVYKI GGIGTVPVG RVETGVLKP 
      GMVVVFAPA NITTEVKSV EMHHEALPE AVPGDNVGF 
      NVKNVSVKE LRRGYVAGD SKASPPKGA ADFLAQVIV 
      LNHPGQISN GYTPVLDCH TAHIACKFA EIKEKVDRR 
      SGKTTEENP KAIKSGDAA IVTLVPSKP MCVESFQEF 
      PPLGRFAVR DMRQTVAVG VIKSVNFKD AGAGKVTKA 
      AEKAAKKK
    CDS sequence
    • >TC031771 RA
      atggaggat gctctcagc attgatagc aagttgatt 
      aagatgggg gccaccggg aacccgaac ataactcgc 
      ctcgccacc ctgcaagag cggaagaaa acgatcgag 
      gaaagtcta gccaaatgc aaccaggaa ttgaaacaa 
      atatgcctc caggaggcc gacttgacc gggataatc 
      cccacggaa atgcccctg gagcctgga gagtctccg 
      ccgttgatt cgacgacgt gttggcact gcgtatcag 
      ttacctgaa aatttaatc aagtcgagt aaagatgag 
      tcgcttaaa gacctagag ctgcaaata caggtgcat 
      gctaatatg gccgaggct gcactaggc ctagccaat 
      gagcaaaat attagcaag acattgcgt cgccaacac 
      atggccgag tatcagcag cataaaata cagtttacg 
      gcgcttcag gagaaatta tcggcgttg aaagataaa 
      gcgcaacaa aatgagtta cataaacag aagaaaaaa 
      ccaagaccg gccgaacaa gatgacaac gtatcgttg 
      ggcactaac atgaatgaa cctttcggc aagtctgac 
      ctgagacat agtatgagg tctaacaag tgtgacact 
      gatgttgaa caaaggtac ctgctttca aaccctaga 
      atgacttac aaaaacgtt actaactcg tacatggag 
      ggttcgttt acccggcaa gaggagagc atcgcgcgt 
      ggtttatac gctttgagc ttcaacgga tataaaaac 
      tacatcgag cgacaagaa agtctagcc aacaatcac 
      attggcggt tatagcact ttacaatac agccaactc 
      caacactcg cccgtcaac tcgcactac tcccagtcc 
      taccagcca aacccacga ccaaatact ctctacaat 
      taccccgtt aaccagaat ttccaagtt cctcaagtc 
      atccaacat cacagcccc caatcgacg cgatcgcaa 
      caccggcag tctagtccc aacctcaac acaacaaaa 
      atgttctac gcccaaaac cccgactac agattgcac 
      tccgaaaac ccgcaccaa atacagccc caccagcag 
      tacgaacac atgaccctg ggcttgggg ggctactgg 
      aagcggcta gacagcggc cagacggtt tggtgcaac 
      tctagcccc actgaatcc aactggcaa agggacaag 
      aggtttgga agcttggat cgcaggaaa aacaagagg 
      ttgcacaag cgagtctca ccgtcggtt agtagcaag 
      tcggcgacg cttgccact gttccggtg tatgctgag 
      cagatgcgg actccgtcc atcaaatcg cctcaagtg 
      attagtagg aggtctcag gataatagg cagctagtt 
      agaacccaa tcacttggt agtgtaggg ggccaaact 
      gttgacagt gtctggccg agtgatgat aactcatct 
      tgtgaaagt gataatcgt agtattaat gaagcgggg 
      caaaacgcc cggaagcag aagcaaaaa gaatggatg 
      gagacgttc ctcgacggc ccggtctcc cctacacac 
      tccgttatc tcgcgttca caatcagcg ttaccttcg 
      gtgttatca cccgaagag aagtacatc gtgagcccc 
      cctcaaccc atcccgcct ccacaaacc cctaaaccg 
      cccctggaa attccagcc gaatcaaat ccgtccccg 
      cgtatacct gaaacaaat atagaatta tttaataac 
      aatattccc aaaaactgt actatcgtc caagccggt 
      cattacaag ccgtaccat gaggaaacc aagccgttt 
      gaaatgtcc gactattac aaatactcg gcaaagttc 
      aaaaagtcg ccccagaag cagcaggaa gacactttg 
      aaaaagtcg caaggcggg gcctcgttt cagaagaat 
      ttaaacgaa gcgtttgat aagccggtg aaaaaccac 
      aacttcagc ccgggatcg ccgcgtttg gacaattcg 
      gtcaattcg agcttcgat ctcagccag aatgttgcc 
      gaacggttc tccgacgaa atgaacgcc tggtatttt 
      gttaactgt tgcatttat ttcattcca aacggttgg 
      agcaagatg ggtaaagaa aagactcat atcaacatc 
      gtcgttatc ggccacgta gattctggt aaatctacc 
      accactggt cacttgatc tacaaatgc ggtggcatc 
      gataagcgt acgatcgaa aagttcgag aaggaggcc 
      caggagatg ggcaagggt tcgttcaaa tacgcctgg 
      gttttggac aagctcaaa gccgagcgt gaacgtggt 
      atcaccatc gatatcgcg ctgtggaaa ttcgaaact 
      tccaaatac tacgttact atcattgac gctcccggc 
      cacagagat ttcatcaag aacatgatc acggggacg 
      tctcaggcc gattgcgcc gttttgatc gtggccgca 
      ggtaccggt gagttcgaa gccggcatc tccaagaac 
      ggacagacc cgcgaacac gcccttctg gccttcact 
      ctgggagtc aaacaactg atcgttggt gtgaacaag 
      atggactcc actgaacca ccatacagc gaagctaga 
      tacgaagaa atcaaaaag gaagtgtct tcctacatc 
      aagaagatc ggctacaac ccagccgct gttgccttc 
      gtacccatc tccgggtgg cacggggac aacatgttg 
      gaaccctcc accaaaatg ccctggttc aaggggtgg 
      aacatcgaa cgcaaggag ggcaaagct gagggcaaa 
      tgcttgatc gaggccctt gatgccatc ctcccgccc 
      acgcgcccc accgacaag cccctccgt cttccgctc 
      caggacgtg tacaagatc ggcggtatt ggaacagta 
      ccagtcggt cgtgtcgaa accggagtc ttgaaaccc 
      ggaatggtg gtcgtgttc gcgcccgct aacatcacg 
      actgaagtc aagtccgtc gaaatgcac cacgaggcg 
      ctcccggag gccgtgccc ggcgacaac gtcggcttc 
      aacgtgaag aacgtgtcc gtgaaggag ttgcgtcgt 
      gggtacgtg gccggcgac tcgaaggcc agccccccg 
      aaaggcgcg gctgacttc ttggcgcaa gttatcgtg 
      ttgaaccac cctggacaa atttcgaac ggttacacc 
      cccgttctt gactgtcac accgctcac atcgcgtgc 
      aagttcgcc gaaatcaaa gaaaaggtc gaccgtcgt 
      tcaggaaag accactgaa gagaatccc aaagctatc 
      aagtctgga gacgctgct attgtcact ttggtaccg 
      tcgaaacca atgtgcgtc gaatcattc caggagttc 
      ccgccattg ggacgtttc gccgtacgt gatatgagg 
      caaaccgtt gccgtaggt gtcatcaag agtgtcaac 
      ttcaaggat gctggtgct gggaaagtc acaaaggcc 
      gcagaaaaa gccgccaag aagaaatga
    mRNA sequence
    • >TC031771 RA
      acagatgag accatgtaa aatggagga tgctctcag 
      cattgatag caagttgat taagatggg ggccaccgg 
      gaacccgaa cataactcg cctcgccac cctgcaaga 
      gcggaagaa aacgatcga ggaaagtct agccaaatg 
      caaccagga attgaaaca aatatgcct ccaggaggc 
      cgacttgac cgggataat ccccacgga aatgcccct 
      ggagcctgg agagtctcc gccgttgat tcgacgacg 
      tgttggcac tgcgtatca gttacctga aaatttaat 
      caagtcgag taaagatga gtcgcttaa agacctaga 
      gctgcaaat acaggtgca tgctaatat ggccgaggc 
      tgcactagg cctagccaa tgagcaaaa tattagcaa 
      gacattgcg tcgccaaca catggccga gtatcagca 
      gcataaaat acagtttac ggcgcttca ggagaaatt 
      atcggcgtt gaaagataa agcgcaaca aaatgagtt 
      acataaaca gaagaaaaa accaagacc ggccgaaca 
      agatgacaa cgtatcgtt gggcactaa catgaatga 
      acctttcgg caagtctga cctgagaca tagtatgag 
      gtctaacaa gtgtgacac tgatgttga acaaaggta 
      cctgctttc aaaccctag aatgactta caaaaacgt 
      tactaactc gtacatgga gggttcgtt tacccggca 
      agaggagag catcgcgcg tggtttata cgctttgag 
      cttcaacgg atataaaaa ctacatcga gcgacaaga 
      aagtctagc caacaatca cattggcgg ttatagcac 
      tttacaata cagccaact ccaacactc gcccgtcaa 
      ctcgcacta ctcccagtc ctaccagcc aaacccacg 
      accaaatac tctctacaa ttaccccgt taaccagaa 
      tttccaagt tcctcaagt catccaaca tcacagccc 
      ccaatcgac gcgatcgca acaccggca gtctagtcc 
      caacctcaa cacaacaaa aatgttcta cgcccaaaa 
      ccccgacta cagattgca ctccgaaaa cccgcacca 
      aatacagcc ccaccagca gtacgaaca catgaccct 
      gggcttggg gggctactg gaagcggct agacagcgg 
      ccagacggt ttggtgcaa ctctagccc cactgaatc 
      caactggca aagggacaa gaggtttgg aagcttgga 
      tcgcaggaa aaacaagag gttgcacaa gcgagtctc 
      accgtcggt tagtagcaa gtcggcgac gcttgccac 
      tgttccggt gtatgctga gcagatgcg gactccgtc 
      catcaaatc gcctcaagt gattagtag gaggtctca 
      ggataatag gcagctagt tagaaccca atcacttgg 
      tagtgtagg gggccaaac tgttgacag tgtctggcc 
      gagtgatga taactcatc ttgtgaaag tgataatcg 
      tagtattaa tgaagcggg gcaaaacgc ccggaagca 
      gaagcaaaa agaatggat ggagacgtt cctcgacgg 
      cccggtctc ccctacaca ctccgttat ctcgcgttc 
      acaatcagc gttaccttc ggtgttatc acccgaaga 
      gaagtacat cgtgagccc ccctcaacc catcccgcc 
      tccacaaac ccctaaacc gcccctgga aattccagc 
      cgaatcaaa tccgtcccc gcgtatacc tgaaacaaa 
      tatagaatt atttaataa caatattcc caaaaactg 
      tactatcgt ccaagccgg tcattacaa gccgtacca 
      tgaggaaac caagccgtt tgaaatgtc cgactatta 
      caaatactc ggcaaagtt caaaaagtc gccccagaa 
      gcagcagga agacacttt gaaaaagtc gcaaggcgg 
      ggcctcgtt tcagaagaa tttaaacga agcgtttga 
      taagccggt gaaaaacca caacttcag cccgggatc 
      gccgcgttt ggacaattc ggtcaattc gagcttcga 
      tctcagcca gaatgttgc cgaacggtt ctccgacga 
      aatgaacgc ctggtattt tgttaactg ttgcattta 
      tttcattcc aaacggttg gagcaagat gggtaaaga 
      aaagactca tatcaacat cgtcgttat cggccacgt 
      agattctgg taaatctac caccactgg tcacttgat 
      ctacaaatg cggtggcat cgataagcg tacgatcga 
      aaagttcga gaaggaggc ccaggagat gggcaaggg 
      ttcgttcaa atacgcctg ggttttgga caagctcaa 
      agccgagcg tgaacgtgg tatcaccat cgatatcgc 
      gctgtggaa attcgaaac ttccaaata ctacgttac 
      tatcattga cgctcccgg ccacagaga tttcatcaa 
      gaacatgat cacggggac gtctcaggc cgattgcgc 
      cgttttgat cgtggccgc aggtaccgg tgagttcga 
      agccggcat ctccaagaa cggacagac ccgcgaaca 
      cgcccttct ggccttcac tctgggagt caaacaact 
      gatcgttgg tgtgaacaa gatggactc cactgaacc 
      accatacag cgaagctag atacgaaga aatcaaaaa 
      ggaagtgtc ttcctacat caagaagat cggctacaa 
      cccagccgc tgttgcctt cgtacccat ctccgggtg 
      gcacgggga caacatgtt ggaaccctc caccaaaat 
      gccctggtt caaggggtg gaacatcga acgcaagga 
      gggcaaagc tgagggcaa atgcttgat cgaggccct 
      tgatgccat cctcccgcc cacgcgccc caccgacaa 
      gcccctccg tcttccgct ccaggacgt gtacaagat 
      cggcggtat tggaacagt accagtcgg tcgtgtcga 
      aaccggagt cttgaaacc cggaatggt ggtcgtgtt 
      cgcgcccgc taacatcac gactgaagt caagtccgt 
      cgaaatgca ccacgaggc gctcccgga ggccgtgcc 
      cggcgacaa cgtcggctt caacgtgaa gaacgtgtc 
      cgtgaagga gttgcgtcg tgggtacgt ggccggcga 
      ctcgaaggc cagcccccc gaaaggcgc ggctgactt 
      cttggcgca agttatcgt gttgaacca ccctggaca 
      aatttcgaa cggttacac ccccgttct tgactgtca 
      caccgctca catcgcgtg caagttcgc cgaaatcaa 
      agaaaaggt cgaccgtcg ttcaggaaa gaccactga 
      agagaatcc caaagctat caagtctgg agacgctgc 
      tattgtcac tttggtacc gtcgaaacc aatgtgcgt 
      cgaatcatt ccaggagtt cccgccatt gggacgttt 
      cgccgtacg tgatatgag gcaaaccgt tgccgtagg 
      tgtcatcaa gagtgtcaa cttcaagga tgctggtgc 
      tgggaaagt cacaaaggc cgcagaaaa agccgccaa 
      gaagaaatg agcttaaac tccgtttat tcattttat 
      aagttcagt tttcaataa atttggagt tttaaagat 
      attttattt tataacttg tgattttat agaataatt 
      ccacagttt gacatttcc atgtttatt acttactaa 
      taaatgatt tcgtaaatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY