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Gene information
General information
TC001878 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 278810..283689
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001878
    iBeetle screen iB_03264

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001878 RA
      MASNDESSS DDENNRFFK KGSVSRTDE CITCCKNFD 
      RSVEKCSCE VSNNLNQNG QETKTLPEN PPVTLTNGQ 
      IKSDLPENN HLTSKENSS DSDTVTNQK SSEVSLTES 
      IKPETSDFL PHFKEENGV TEVNQNSFD SDDKSVSEN 
      GCDQSCNIG AVNLTIDTN NGSLNQETS KTSGLQINS 
      NVNNNDILN DLNLTNDSN KFKPKSPCT PVKTELPAI 
      EEQHFTSQV NCSPRNART RSVETATLP KKTAKKRAL 
      SADADHRGV KLRRLDFNK AKPSATPTK NEHKDDRSK 
      SRRHESTKK HDKRRSSSS RRKTVGTQT RPRDLAVEP 
      SLMTNGNYS HPPADTNLK YRRFYHIET HSNGGAKIL 
      RMYDDEIKH LRQEEQVEL AQEFFTLAF KENSDGHAV 
      FVIAVVHGS ARYLPDILE YMAEKYPNL TVKNGLLNK 
      SSDIETTTF SEYNKNVIK HYESGTVRY GPLHQISIV 
      GTAHEEVGG YFPDLLDML EANHFLKLT MPWGRLSIC 
      RDMKRTESN DGPIVWCRP GEQLVPTAD SRTPLKRKR 
      TGINELKNL QYLPRMSEA REHLFEDRT KAHADHVGA 
      GLDRKTTAA VGILKAIHG GQNEGPINR ITKDVVAFS 
      AKYFDILAE KLQLDLHEP PISQCVTWI EDAKLNQLR 
      REGIEYARV NLYDNDIYF LPRNIIHQF RTVTAVTSI 
      AWHVRLQQY YPTCETKNF DENEINNKV LIRNDSCES 
      SKNNHVKNV LKVKQKLDF EKQVIETDH KYKYKDKNR 
      SKDKIRDEN KEKREKDRD KHSHKSRDK DRHEHKDKK 
      YDKDKDRHH YHNHKEHKS KDEKSRHHS HKDKYKSSS 
      EHKSRHKSS HSHHKDAKK APSEKNNCI KTSVSSSTS 
      SLSSTSVPK TPSTPNSND NTPMQRLLG DSLTSPLKH 
      KSEKKPQKI IKRPRLSQS ADVLGDILK DMNKCDPHI
    CDS sequence
    • >TC001878 RA
      atggcgtcg aatgacgaa tcttcgagt gacgatgaa 
      aataataga ttttttaaa aaagggtcg gtttcacga 
      acagacgag tgcatcact tgttgcaaa aattttgat 
      agaagcgtc gaaaagtgc tcttgcgaa gtgtccaac 
      aaccttaac caaaatgga caagaaaca aaaactttg 
      cctgaaaat cccccagta acgttaaca aacggccaa 
      atcaagtcg gatttgccg gagaacaat catcttact 
      tccaaggaa aactctagt gatagtgat acagtgacg 
      aatcaaaaa tccagtgaa gtgagtttg acggagtca 
      atcaagcct gaaacgtcc gatttttta ccacacttc 
      aaggaagag aacggtgtg actgaagtt aaccaaaac 
      tcgtttgat agtgacgat aagtcagtt tctgaaaac 
      ggctgtgat caaagttgc aatattggt gctgtgaat 
      cttaccata gacactaat aacggttct ctaaatcag 
      gaaacttca aaaaccagt ggccttcag ataaattca 
      aatgttaat aataacgat atattaaac gacttaaat 
      ttaacaaat gacagcaat aaattcaaa ccaaagagc 
      ccttgtacc cccgtcaag accgaacta ccagccatt 
      gaagaacaa catttcaca agccaagtc aattgttcg 
      ccaagaaat gcacgcacg agatccgtc gaaacggcc 
      actcttccc aaaaagacc gccaaaaaa cgcgccctc 
      tcggccgac gccgaccac aggggcgtc aagctgcgg 
      aggctcgac ttcaacaag gccaaacca agcgccaca 
      ccgaccaaa aacgaacac aaggacgac agaagcaag 
      tccagaaga cacgaaagt acaaaaaag cacgacaag 
      agaagaagc agctcttcc aggaggaag accgtcggc 
      acccagacg aggccgcgc gatctggcc gtcgagccc 
      tccctgatg accaacggc aactacagc caccccccg 
      gccgatacg aacttgaag taccgccgt ttctaccac 
      atcgagacg cattcgaac ggaggggcc aaaattttg 
      cgaatgtac gacgacgaa atcaagcat ttgcgtcaa 
      gaggagcaa gtggagttg gcccaggag ttttttact 
      ctcgctttt aaggaaaat tcggacggt catgctgtg 
      tttgttatc gctgtggtc cacggttcg gctcggtat 
      ctccccgat attttggaa tacatggcc gagaaatac 
      ccgaatttg actgtgaag aacggcctt ttgaacaaa 
      agttccgac atcgaaacg accactttt tcggagtat 
      aacaagaat gttatcaag cattacgaa agtgggacg 
      gttcgatac ggtcctttg caccagata agtatcgtt 
      gggacggcc catgaagaa gtggggggc tatttcccc 
      gacttgttg gacatgcta gaagcgaac catttttta 
      aaacttaca atgccttgg ggccgactg tcgatttgt 
      cgcgacatg aaacgaacc gagtcgaac gatggccca 
      attgtctgg tgcagacca ggcgaacag ttagtacca 
      acagctgat tcaagaaca ccattaaaa agaaaacgg 
      actggcata aacgaactt aaaaatctc cagtactta 
      ccgcgcatg tctgaagcg cgagaacac ttgtttgag 
      gaccgcacc aaagcccac gctgaccac gttggcgct 
      ggattggac cgcaaaacc actgcggcg gtgggcatc 
      cttaaagca atacacggg gggcaaaat gaaggacca 
      atcaatcga attactaaa gacgttgtg gctttttcc 
      gccaaatat ttcgatatt ctcgcggaa aaactgcaa 
      ctggatttg cacgaaccg ccgatttca cagtgtgtt 
      acttggatc gaagatgcc aaactcaac cagttaagg 
      agagaaggt atcgagtat gctagagtg aatttgtac 
      gataacgat atatacttt ttgccaaga aatatcata 
      caccagttt cggaccgtg acggcggtg acgagcata 
      gcatggcat gtgcgtctc caacagtat taccccact 
      tgcgaaacc aaaaacttc gacgagaac gaaataaac 
      aacaaagtc ttaatcagg aacgactcc tgcgagtcg 
      tcgaaaaac aaccacgtg aagaacgtc ctcaaagtg 
      aagcaaaag ctcgacttc gagaaacaa gtaatcgag 
      accgaccac aaatacaaa tacaaggac aaaaaccgc 
      tcgaaggac aaaatccgg gacgaaaac aaggaaaag 
      cgggagaaa gatcgcgac aagcacagc cacaagtcg 
      cgcgataag gaccgacac gagcacaaa gacaagaaa 
      tacgataaa gacaaagac cgccaccac taccacaac 
      cacaaagaa cacaagtcg aaagacgaa aagtcgcgt 
      caccactcg cataaagac aagtataag agcagtagc 
      gagcataag agtcgccat aaaagtagt cactcgcac 
      cacaaagat gcgaagaaa gccccgagt gagaaaaat 
      aattgcatc aaaacctcg gtctcgagt agcacgagc 
      tcgctctcg agcacgtcg gtgccgaaa acaccgtcg 
      acgcccaac agcaatgat aatactccc atgcagagg 
      cttctgggg gacagtctc acgtcgcct ttgaagcac 
      aagtcggag aaaaagccg cagaaaatt atcaaacgt 
      ccacgactg tcacaatca gccgatgtc ctcggcgat 
      attctgaaa gacatgaac aagtgcgac ccgcatata 
      taa
    mRNA sequence
    • >TC001878 RA
      cggttacga ggagcatgg cggcaagcg tggaggttt 
      tctagtttt acacgtttt ttgtacgta ttttaaaag 
      taattatcg ttgattttg aaaaattac tcgtgttgt 
      gttacggtt ttagtgtaa gcgataatt atgtgactg 
      tttgtatat ccgatatac gcaaaagtt tgtaaataa 
      acagtaacc cgggctttt acaatcatg gcgtcgaat 
      gacgaatct tcgagtgac gatgaaaat aatagattt 
      tttaaaaaa gggtcggtt tcacgaaca gacgagtgc 
      atcacttgt tgcaaaaat tttgataga agcgtcgaa 
      aagtgctct tgcgaagtg tccaacaac cttaaccaa 
      aatggacaa gaaacaaaa actttgcct gaaaatccc 
      ccagtaacg ttaacaaac ggccaaatc aagtcggat 
      ttgccggag aacaatcat cttacttcc aaggaaaac 
      tctagtgat agtgataca gtgacgaat caaaaatcc 
      agtgaagtg agtttgacg gagtcaatc aagcctgaa 
      acgtccgat tttttacca cacttcaag gaagagaac 
      ggtgtgact gaagttaac caaaactcg tttgatagt 
      gacgataag tcagtttct gaaaacggc tgtgatcaa 
      agttgcaat attggtgct gtgaatctt accatagac 
      actaataac ggttctcta aatcaggaa acttcaaaa 
      accagtggc cttcagata aattcaaat gttaataat 
      aacgatata ttaaacgac ttaaattta acaaatgac 
      agcaataaa ttcaaacca aagagccct tgtaccccc 
      gtcaagacc gaactacca gccattgaa gaacaacat 
      ttcacaagc caagtcaat tgttcgcca agaaatgca 
      cgcacgaga tccgtcgaa acggccact cttcccaaa 
      aagaccgcc aaaaaacgc gccctctcg gccgacgcc 
      gaccacagg ggcgtcaag ctgcggagg ctcgacttc 
      aacaaggcc aaaccaagc gccacaccg accaaaaac 
      gaacacaag gacgacaga agcaagtcc agaagacac 
      gaaagtaca aaaaagcac gacaagaga agaagcagc 
      tcttccagg aggaagacc gtcggcacc cagacgagg 
      ccgcgcgat ctggccgtc gagccctcc ctgatgacc 
      aacggcaac tacagccac cccccggcc gatacgaac 
      ttgaagtac cgccgtttc taccacatc gagacgcat 
      tcgaacgga ggggccaaa attttgcga atgtacgac 
      gacgaaatc aagcatttg cgtcaagag gagcaagtg 
      gagttggcc caggagttt tttactctc gcttttaag 
      gaaaattcg gacggtcat gctgtgttt gttatcgct 
      gtggtccac ggttcggct cggtatctc cccgatatt 
      ttggaatac atggccgag aaatacccg aatttgact 
      gtgaagaac ggccttttg aacaaaagt tccgacatc 
      gaaacgacc actttttcg gagtataac aagaatgtt 
      atcaagcat tacgaaagt gggacggtt cgatacggt 
      cctttgcac cagataagt atcgttggg acggcccat 
      gaagaagtg gggggctat ttccccgac ttgttggac 
      atgctagaa gcgaaccat tttttaaaa cttacaatg 
      ccttggggc cgactgtcg atttgtcgc gacatgaaa 
      cgaaccgag tcgaacgat ggcccaatt gtctggtgc 
      agaccaggc gaacagtta gtaccaaca gctgattca 
      agaacacca ttaaaaaga aaacggact ggcataaac 
      gaacttaaa aatctccag tacttaccg cgcatgtct 
      gaagcgcga gaacacttg tttgaggac cgcaccaaa 
      gcccacgct gaccacgtt ggcgctgga ttggaccgc 
      aaaaccact gcggcggtg ggcatcctt aaagcaata 
      cacgggggg caaaatgaa ggaccaatc aatcgaatt 
      actaaagac gttgtggct ttttccgcc aaatatttc 
      gatattctc gcggaaaaa ctgcaactg gatttgcac 
      gaaccgccg atttcacag tgtgttact tggatcgaa 
      gatgccaaa ctcaaccag ttaaggaga gaaggtatc 
      gagtatgct agagtgaat ttgtacgat aacgatata 
      tactttttg ccaagaaat atcatacac cagtttcgg 
      accgtgacg gcggtgacg agcatagca tggcatgtg 
      cgtctccaa cagtattac cccacttgc gaaaccaaa 
      aacttcgac gagaacgaa ataaacaac aaagtctta 
      atcaggaac gactcctgc gagtcgtcg aaaaacaac 
      cacgtgaag aacgtcctc aaagtgaag caaaagctc 
      gacttcgag aaacaagta atcgagacc gaccacaaa 
      tacaaatac aaggacaaa aaccgctcg aaggacaaa 
      atccgggac gaaaacaag gaaaagcgg gagaaagat 
      cgcgacaag cacagccac aagtcgcgc gataaggac 
      cgacacgag cacaaagac aagaaatac gataaagac 
      aaagaccgc caccactac cacaaccac aaagaacac 
      aagtcgaaa gacgaaaag tcgcgtcac cactcgcat 
      aaagacaag tataagagc agtagcgag cataagagt 
      cgccataaa agtagtcac tcgcaccac aaagatgcg 
      aagaaagcc ccgagtgag aaaaataat tgcatcaaa 
      acctcggtc tcgagtagc acgagctcg ctctcgagc 
      acgtcggtg ccgaaaaca ccgtcgacg cccaacagc 
      aatgataat actcccatg cagaggctt ctgggggac 
      agtctcacg tcgcctttg aagcacaag tcggagaaa 
      aagccgcag aaaattatc aaacgtcca cgactgtca 
      caatcagcc gatgtcctc ggcgatatt ctgaaagac 
      atgaacaag tgcgacccg catatataa cagtaactt 
      gtcatgttg tgtatatag ttgtaaata gtctggcgt 
      tagctggga tgaagtgtc atccgcctg aattctaaa 
      gagagttgg acggcttgt ccaagtttt acgaatctc 
      gcctgtttt agccaggct tttggtaat ttatttatt 
      ttgtttcat cgtgtagcg cgtactaga aattatcga 
      tctcattta ttgtgtttt gccattact aatgtattc 
      tagttaggc ctttttaaa tgttttatt tatggttct 
      aattgtaca tatgtatag gtagagaaa tatgtacag 
      atttttacg tagtcaaga ccttataag cgttgctgt 
      aaaaattca aatatataa caatgttag ag
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY