| General information | |||
| TC001878 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 278810..283689 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001878 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03264 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001878 RA
MASNDESSS DDENNRFFK KGSVSRTDE CITCCKNFD RSVEKCSCE VSNNLNQNG QETKTLPEN PPVTLTNGQ IKSDLPENN HLTSKENSS DSDTVTNQK SSEVSLTES IKPETSDFL PHFKEENGV TEVNQNSFD SDDKSVSEN GCDQSCNIG AVNLTIDTN NGSLNQETS KTSGLQINS NVNNNDILN DLNLTNDSN KFKPKSPCT PVKTELPAI EEQHFTSQV NCSPRNART RSVETATLP KKTAKKRAL SADADHRGV KLRRLDFNK AKPSATPTK NEHKDDRSK SRRHESTKK HDKRRSSSS RRKTVGTQT RPRDLAVEP SLMTNGNYS HPPADTNLK YRRFYHIET HSNGGAKIL RMYDDEIKH LRQEEQVEL AQEFFTLAF KENSDGHAV FVIAVVHGS ARYLPDILE YMAEKYPNL TVKNGLLNK SSDIETTTF SEYNKNVIK HYESGTVRY GPLHQISIV GTAHEEVGG YFPDLLDML EANHFLKLT MPWGRLSIC RDMKRTESN DGPIVWCRP GEQLVPTAD SRTPLKRKR TGINELKNL QYLPRMSEA REHLFEDRT KAHADHVGA GLDRKTTAA VGILKAIHG GQNEGPINR ITKDVVAFS AKYFDILAE KLQLDLHEP PISQCVTWI EDAKLNQLR REGIEYARV NLYDNDIYF LPRNIIHQF RTVTAVTSI AWHVRLQQY YPTCETKNF DENEINNKV LIRNDSCES SKNNHVKNV LKVKQKLDF EKQVIETDH KYKYKDKNR SKDKIRDEN KEKREKDRD KHSHKSRDK DRHEHKDKK YDKDKDRHH YHNHKEHKS KDEKSRHHS HKDKYKSSS EHKSRHKSS HSHHKDAKK APSEKNNCI KTSVSSSTS SLSSTSVPK TPSTPNSND NTPMQRLLG DSLTSPLKH KSEKKPQKI IKRPRLSQS ADVLGDILK DMNKCDPHI
- >TC001878 RA
atggcgtcg aatgacgaa tcttcgagt gacgatgaa aataataga ttttttaaa aaagggtcg gtttcacga acagacgag tgcatcact tgttgcaaa aattttgat agaagcgtc gaaaagtgc tcttgcgaa gtgtccaac aaccttaac caaaatgga caagaaaca aaaactttg cctgaaaat cccccagta acgttaaca aacggccaa atcaagtcg gatttgccg gagaacaat catcttact tccaaggaa aactctagt gatagtgat acagtgacg aatcaaaaa tccagtgaa gtgagtttg acggagtca atcaagcct gaaacgtcc gatttttta ccacacttc aaggaagag aacggtgtg actgaagtt aaccaaaac tcgtttgat agtgacgat aagtcagtt tctgaaaac ggctgtgat caaagttgc aatattggt gctgtgaat cttaccata gacactaat aacggttct ctaaatcag gaaacttca aaaaccagt ggccttcag ataaattca aatgttaat aataacgat atattaaac gacttaaat ttaacaaat gacagcaat aaattcaaa ccaaagagc ccttgtacc cccgtcaag accgaacta ccagccatt gaagaacaa catttcaca agccaagtc aattgttcg ccaagaaat gcacgcacg agatccgtc gaaacggcc actcttccc aaaaagacc gccaaaaaa cgcgccctc tcggccgac gccgaccac aggggcgtc aagctgcgg aggctcgac ttcaacaag gccaaacca agcgccaca ccgaccaaa aacgaacac aaggacgac agaagcaag tccagaaga cacgaaagt acaaaaaag cacgacaag agaagaagc agctcttcc aggaggaag accgtcggc acccagacg aggccgcgc gatctggcc gtcgagccc tccctgatg accaacggc aactacagc caccccccg gccgatacg aacttgaag taccgccgt ttctaccac atcgagacg cattcgaac ggaggggcc aaaattttg cgaatgtac gacgacgaa atcaagcat ttgcgtcaa gaggagcaa gtggagttg gcccaggag ttttttact ctcgctttt aaggaaaat tcggacggt catgctgtg tttgttatc gctgtggtc cacggttcg gctcggtat ctccccgat attttggaa tacatggcc gagaaatac ccgaatttg actgtgaag aacggcctt ttgaacaaa agttccgac atcgaaacg accactttt tcggagtat aacaagaat gttatcaag cattacgaa agtgggacg gttcgatac ggtcctttg caccagata agtatcgtt gggacggcc catgaagaa gtggggggc tatttcccc gacttgttg gacatgcta gaagcgaac catttttta aaacttaca atgccttgg ggccgactg tcgatttgt cgcgacatg aaacgaacc gagtcgaac gatggccca attgtctgg tgcagacca ggcgaacag ttagtacca acagctgat tcaagaaca ccattaaaa agaaaacgg actggcata aacgaactt aaaaatctc cagtactta ccgcgcatg tctgaagcg cgagaacac ttgtttgag gaccgcacc aaagcccac gctgaccac gttggcgct ggattggac cgcaaaacc actgcggcg gtgggcatc cttaaagca atacacggg gggcaaaat gaaggacca atcaatcga attactaaa gacgttgtg gctttttcc gccaaatat ttcgatatt ctcgcggaa aaactgcaa ctggatttg cacgaaccg ccgatttca cagtgtgtt acttggatc gaagatgcc aaactcaac cagttaagg agagaaggt atcgagtat gctagagtg aatttgtac gataacgat atatacttt ttgccaaga aatatcata caccagttt cggaccgtg acggcggtg acgagcata gcatggcat gtgcgtctc caacagtat taccccact tgcgaaacc aaaaacttc gacgagaac gaaataaac aacaaagtc ttaatcagg aacgactcc tgcgagtcg tcgaaaaac aaccacgtg aagaacgtc ctcaaagtg aagcaaaag ctcgacttc gagaaacaa gtaatcgag accgaccac aaatacaaa tacaaggac aaaaaccgc tcgaaggac aaaatccgg gacgaaaac aaggaaaag cgggagaaa gatcgcgac aagcacagc cacaagtcg cgcgataag gaccgacac gagcacaaa gacaagaaa tacgataaa gacaaagac cgccaccac taccacaac cacaaagaa cacaagtcg aaagacgaa aagtcgcgt caccactcg cataaagac aagtataag agcagtagc gagcataag agtcgccat aaaagtagt cactcgcac cacaaagat gcgaagaaa gccccgagt gagaaaaat aattgcatc aaaacctcg gtctcgagt agcacgagc tcgctctcg agcacgtcg gtgccgaaa acaccgtcg acgcccaac agcaatgat aatactccc atgcagagg cttctgggg gacagtctc acgtcgcct ttgaagcac aagtcggag aaaaagccg cagaaaatt atcaaacgt ccacgactg tcacaatca gccgatgtc ctcggcgat attctgaaa gacatgaac aagtgcgac ccgcatata taa
- >TC001878 RA
cggttacga ggagcatgg cggcaagcg tggaggttt tctagtttt acacgtttt ttgtacgta ttttaaaag taattatcg ttgattttg aaaaattac tcgtgttgt gttacggtt ttagtgtaa gcgataatt atgtgactg tttgtatat ccgatatac gcaaaagtt tgtaaataa acagtaacc cgggctttt acaatcatg gcgtcgaat gacgaatct tcgagtgac gatgaaaat aatagattt tttaaaaaa gggtcggtt tcacgaaca gacgagtgc atcacttgt tgcaaaaat tttgataga agcgtcgaa aagtgctct tgcgaagtg tccaacaac cttaaccaa aatggacaa gaaacaaaa actttgcct gaaaatccc ccagtaacg ttaacaaac ggccaaatc aagtcggat ttgccggag aacaatcat cttacttcc aaggaaaac tctagtgat agtgataca gtgacgaat caaaaatcc agtgaagtg agtttgacg gagtcaatc aagcctgaa acgtccgat tttttacca cacttcaag gaagagaac ggtgtgact gaagttaac caaaactcg tttgatagt gacgataag tcagtttct gaaaacggc tgtgatcaa agttgcaat attggtgct gtgaatctt accatagac actaataac ggttctcta aatcaggaa acttcaaaa accagtggc cttcagata aattcaaat gttaataat aacgatata ttaaacgac ttaaattta acaaatgac agcaataaa ttcaaacca aagagccct tgtaccccc gtcaagacc gaactacca gccattgaa gaacaacat ttcacaagc caagtcaat tgttcgcca agaaatgca cgcacgaga tccgtcgaa acggccact cttcccaaa aagaccgcc aaaaaacgc gccctctcg gccgacgcc gaccacagg ggcgtcaag ctgcggagg ctcgacttc aacaaggcc aaaccaagc gccacaccg accaaaaac gaacacaag gacgacaga agcaagtcc agaagacac gaaagtaca aaaaagcac gacaagaga agaagcagc tcttccagg aggaagacc gtcggcacc cagacgagg ccgcgcgat ctggccgtc gagccctcc ctgatgacc aacggcaac tacagccac cccccggcc gatacgaac ttgaagtac cgccgtttc taccacatc gagacgcat tcgaacgga ggggccaaa attttgcga atgtacgac gacgaaatc aagcatttg cgtcaagag gagcaagtg gagttggcc caggagttt tttactctc gcttttaag gaaaattcg gacggtcat gctgtgttt gttatcgct gtggtccac ggttcggct cggtatctc cccgatatt ttggaatac atggccgag aaatacccg aatttgact gtgaagaac ggccttttg aacaaaagt tccgacatc gaaacgacc actttttcg gagtataac aagaatgtt atcaagcat tacgaaagt gggacggtt cgatacggt cctttgcac cagataagt atcgttggg acggcccat gaagaagtg gggggctat ttccccgac ttgttggac atgctagaa gcgaaccat tttttaaaa cttacaatg ccttggggc cgactgtcg atttgtcgc gacatgaaa cgaaccgag tcgaacgat ggcccaatt gtctggtgc agaccaggc gaacagtta gtaccaaca gctgattca agaacacca ttaaaaaga aaacggact ggcataaac gaacttaaa aatctccag tacttaccg cgcatgtct gaagcgcga gaacacttg tttgaggac cgcaccaaa gcccacgct gaccacgtt ggcgctgga ttggaccgc aaaaccact gcggcggtg ggcatcctt aaagcaata cacgggggg caaaatgaa ggaccaatc aatcgaatt actaaagac gttgtggct ttttccgcc aaatatttc gatattctc gcggaaaaa ctgcaactg gatttgcac gaaccgccg atttcacag tgtgttact tggatcgaa gatgccaaa ctcaaccag ttaaggaga gaaggtatc gagtatgct agagtgaat ttgtacgat aacgatata tactttttg ccaagaaat atcatacac cagtttcgg accgtgacg gcggtgacg agcatagca tggcatgtg cgtctccaa cagtattac cccacttgc gaaaccaaa aacttcgac gagaacgaa ataaacaac aaagtctta atcaggaac gactcctgc gagtcgtcg aaaaacaac cacgtgaag aacgtcctc aaagtgaag caaaagctc gacttcgag aaacaagta atcgagacc gaccacaaa tacaaatac aaggacaaa aaccgctcg aaggacaaa atccgggac gaaaacaag gaaaagcgg gagaaagat cgcgacaag cacagccac aagtcgcgc gataaggac cgacacgag cacaaagac aagaaatac gataaagac aaagaccgc caccactac cacaaccac aaagaacac aagtcgaaa gacgaaaag tcgcgtcac cactcgcat aaagacaag tataagagc agtagcgag cataagagt cgccataaa agtagtcac tcgcaccac aaagatgcg aagaaagcc ccgagtgag aaaaataat tgcatcaaa acctcggtc tcgagtagc acgagctcg ctctcgagc acgtcggtg ccgaaaaca ccgtcgacg cccaacagc aatgataat actcccatg cagaggctt ctgggggac agtctcacg tcgcctttg aagcacaag tcggagaaa aagccgcag aaaattatc aaacgtcca cgactgtca caatcagcc gatgtcctc ggcgatatt ctgaaagac atgaacaag tgcgacccg catatataa cagtaactt gtcatgttg tgtatatag ttgtaaata gtctggcgt tagctggga tgaagtgtc atccgcctg aattctaaa gagagttgg acggcttgt ccaagtttt acgaatctc gcctgtttt agccaggct tttggtaat ttatttatt ttgtttcat cgtgtagcg cgtactaga aattatcga tctcattta ttgtgtttt gccattact aatgtattc tagttaggc ctttttaaa tgttttatt tatggttct aattgtaca tatgtatag gtagagaaa tatgtacag atttttacg tagtcaaga ccttataag cgttgctgt aaaaattca aatatataa caatgttag ag
