Status
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Gene information
General information
TC001874 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 261171..272568
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001874
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03263

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (11 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001874 RA
      MDLKRWGHF YMKVFLLFG TGGLVLAME PMCSPGLDF 
      GGYTVPQGD IVIEYGNPL DIICVLLNN SKNILGPDA 
      SLNLSFVRN QDAVPPEMI EVINSTAIK LHIDKPPKV 
      SSDTYSCLQ YNKSAVCMN CVTVGTKPQ PVTDFECIS 
      YNYDNLTCT WTPPENYIK TTYNLTFTL GGRAARYIQ 
      ACPHIDYSE KTKRMSCMW NVSTTPQYR QVHEVYNFN 
      LTMENKFGT HRQSYPFQH FAHVLSGPP ENLTVINTT 
      SSSIYLYWM VPLSMRTFW PGVHHRILY QCEYYEKKW 
      HFGGEIKEN PKRYEIYFN LTNLKYAHA LCDIRVSLR 
      SAKALPSDE SMWSNNASI TVWTSSKIP DEPPETNVG 
      SFEVVAFGN SLQNREAYV YWKQIKEEQ KNGPDFHYG 
      IAVEGEPAI RPVEFTNTY AKFENLSIS TSYTFNIWS 
      ENSNGTSAR KSSVFIPKQ TDRIKEPWS FTKVEFGDG 
      VYELTWVAP KLLDNQYIT NYTIFSCSN ERDRPYQCN 
      GMLNWTVVS SNTTSKTMI VEKNKIFQF AISANTHNG 
      SSGMLWASC TVIHNKNMG KMKNVWINS VGSTFIEVG 
      WKLDCADRI GSVEGYVVY YCPIKSPMH TECKAPQAN 
      RTFDGTSML SSGNITDLN PYTTYMLTV SVKTKHTTF 
      SQQSDPLYN TTLEDAPST PPRDVTIQK VTNSSIRVA 
      WKPPEALNG IIRDYVVYC CVKGSLCEK IPVKTELNY 
      TITGLKSFE NYSIELEAC TTKCSGKSE RLQVTTSMG 
      YPSKIRKPT VDLRNDTSI TIVWNEPEE PSGRNDYYV 
      VQFRQKHKS ENKTFQYNT TKRKFVIEN CGDGGKFNA 
      FYISVKAVN KIDNRSYDG PWSDELENY CETPINYIM 
      YLLLFVAIV AVVSVGFFS TRLYNHCKF MRDVEVKLP 
      PGLAPVVAD HTLVPWSTE KRQDPGDSH SPPDEELLL 
      VKMSEGRHF SGDSSGCSS GHESVTSSL ESGTHISSS 
      SDSGTEQPA AYSVMEASS DTPYISVTG GSEASSPYV 
      MTGDAVKTI NPGYIPFTQ PPDFGGGLD AKGDSPYIS 
      VTATDATKM GYKPSSEVS DESSPYVMT GDAPKTINP 
      GYIPFNQTE PMKNSYVHI TF
    CDS sequence
    • >TC001874 RA
      atggattta aagagatgg ggtcacttc tatatgaaa 
      gttttcctt ttgttcggc accggtgga cttgtttta 
      gccatggaa ccgatgtgt tcgccgggc ttagatttc 
      ggagggtac acggtaccc caaggagac atcgtaata 
      gaatacgga aatccatta gatataata tgcgtctta 
      cttaataat agtaaaaat atactcggc cccgatgct 
      agtcttaat ttatcgttt gtgagaaac caggatgcg 
      gttccgccc gaaatgatc gaagtgata aactccaca 
      gccattaaa ttacacatt gataagcca cctaaagta 
      tcaagtgat acctactcc tgtcttcaa tataacaaa 
      agtgcggtt tgtatgaac tgtgtcact gttggaacg 
      aaacctcaa cctgtcacc gattttgaa tgcataagc 
      tacaattat gacaacctc acgtgcaca tggacgcct 
      cccgaaaat tacattaaa acaacctac aacctgaca 
      ttcactcta gggggcagg gcagctcgg tacattcaa 
      gcgtgccct catattgac tactccgaa aaaacgaaa 
      cgcatgagc tgcatgtgg aacgtctcc acgacccca 
      caataccgc caagtgcac gaggtgtac aatttcaat 
      ctcacgatg gaaaacaaa ttcggaaca catcggcag 
      agttacccc ttccaacac tttgcgcac gtattgtct 
      ggccctccc gagaatctc acagttata aacacaacc 
      tcgtcttcg atctatctc tactggatg gtgcccctt 
      tccatgcgc acgttctgg ccaggggtg caccaccgg 
      attctctac caatgcgaa tactacgaa aagaagtgg 
      cacttcggc ggcgaaatc aaggaaaac ccaaaaaga 
      tacgagatt tatttcaac ttgaccaac ctaaaatac 
      gcacacgcc ttatgcgac atccgcgtc tcgctaaga 
      tcggccaaa gctttgcct tccgacgag tcaatgtgg 
      tcgaacaac gcctccatc acagtctgg acgagtagc 
      aaaataccg gacgagccg ccggaaaca aacgtcggg 
      agtttcgaa gtggtggcc tttggtaac agcttgcaa 
      aatcgggag gcttacgtt tactggaaa caaatcaaa 
      gaggagcaa aagaacgga cccgatttt cactacggc 
      attgctgtg gaaggcgaa ccggcgata cgaccggtc 
      gaattcacc aacacttac gccaagttc gaaaatttg 
      tccatctcg accagctac acgttcaat atctggtcg 
      gaaaattca aacgggact tcggcgcgc aaatcgtcg 
      gtttttata ccgaaacag acagatcgt attaaggag 
      ccgtggagt ttcactaaa gttgagttc ggggacggt 
      gtctacgaa ttgacttgg gtggcccca aagttgttg 
      gataaccag tacattaca aactacacg attttttcc 
      tgtagcaat gagcgagat aggccctac cagtgcaac 
      ggcatgttg aactggacc gtggtctcg agcaacacg 
      acttcgaaa actatgata gttgagaag aacaaaatt 
      ttccaattt gcgatttcg gcaaacacg cataacggg 
      agcagcggc atgttgtgg gcgtcctgt accgtcatc 
      cacaacaaa aatatgggc aaaatgaaa aatgtttgg 
      ataaatagc gtcggctca actttcata gaagtgggc 
      tggaagctg gactgcgcc gatcggatc ggcagcgtc 
      gaaggttac gttgtttat tattgcccg atcaaatcg 
      ccaatgcat acggaatgc aaagcgcct caagccaat 
      cgcaccttc gacggaact tcgatgctc tcaagcgga 
      aatataacc gatctgaac ccttacacg acatacatg 
      ttgacggtt tcggtgaaa accaagcat acgactttt 
      agccaacaa agcgatcct ttgtacaac accacgctg 
      gaagatgct cccagcaca cctccgcgt gatgtgact 
      atacaaaaa gttacgaat tcgtcgatt agagtcgcg 
      tggaagcca cccgaagct ctcaatggc ataattcgc 
      gattatgtt gtttattgt tgcgttaaa ggctcgctc 
      tgcgagaaa attccagtc aaaacggaa ctaaactac 
      acgataact ggcctcaaa agcttcgaa aactattcg 
      atagaactc gaggcttgt acgacgaag tgttcaggg 
      aagtcggaa agattacaa gtgacgact agcatgggc 
      tatccgagc aaaatccgt aaaccaacg gtggatttg 
      cgtaacgat acttcgatt acgatcgtt tggaatgag 
      ccagaagag cccagtggg cgcaacgat tattacgtt 
      gtacagttc cgtcaaaaa cacaagagt gagaataag 
      actttccaa tacaacaca acgaagcgc aaattcgtg 
      attgagaat tgcggcgat ggtggtaaa ttcaacgcg 
      ttttacata tcagtcaag gctgttaac aaaatagac 
      aatcggagt tacgacggc ccctggagc gacgaactc 
      gaaaactac tgtgaaacc ccaatcaac tacatcatg 
      tacttgctg ctgttcgtc gcaatcgtt gccgtggtc 
      tccgtcggt ttcttctcg actagatta tacaaccac 
      tgcaagttc atgcgtgat gttgaggta aaactcccc 
      cctgggttg gcccccgtg gtggccgac cacacctta 
      gtcccctgg agcacggag aagcggcaa gaccccggc 
      gattcgcac tcgcccccc gacgaggag ctcctgctt 
      gtgaagatg agtgaaggg agacatttt agtggtgat 
      tcgagtggt tgcagttca ggacatgag agtgtgacc 
      tcgtccctc gaatcgggc acgcacatt tcgagcagt 
      tcggactcg gggaccgaa cagccggcg gcctacagc 
      gtgatggaa gcgagttcg gacacgccg tacatttcc 
      gttactggg ggctcggag gccagctcg ccgtacgtc 
      atgacaggg gatgcggtc aagacgatc aacccgggg 
      tacatcccc ttcacccag cccccggac ttcggcggc 
      gggctggac gcgaagggg gactcgccc tacatctcg 
      gtgacggcc acggacgcg accaaaatg gggtacaag 
      cccagtagt gaggtttca gacgagtct tcgccatat 
      gttatgacg ggggacgcg cccaagacg atcaaccct 
      ggttacata ccgttcaat cagactgaa ccgatgaag 
      aattcatat gtacatatt acattttag
    mRNA sequence
    • >TC001874 RA
      aagtattgc tgttttcaa gaatggtgc atcatacaa 
      cttaaattg tttgaaatc gaacaaaaa tggatttaa 
      agagatggg gtcacttct atatgaaag ttttccttt 
      tgttcggca ccggtggac ttgttttag ccatggaac 
      cgatgtgtt cgccgggct tagatttcg gagggtaca 
      cggtacccc aaggagaca tcgtaatag aatacggaa 
      atccattag atataatat gcgtcttac ttaataata 
      gtaaaaata tactcggcc ccgatgcta gtcttaatt 
      tatcgtttg tgagaaacc aggatgcgg ttccgcccg 
      aaatgatcg aagtgataa actccacag ccattaaat 
      tacacattg ataagccac ctaaagtat caagtgata 
      cctactcct gtcttcaat ataacaaaa gtgcggttt 
      gtatgaact gtgtcactg ttggaacga aacctcaac 
      ctgtcaccg attttgaat gcataagct acaattatg 
      acaacctca cgtgcacat ggacgcctc ccgaaaatt 
      acattaaaa caacctaca acctgacat tcactctag 
      ggggcaggg cagctcggt acattcaag cgtgccctc 
      atattgact actccgaaa aaacgaaac gcatgagct 
      gcatgtgga acgtctcca cgaccccac aataccgcc 
      aagtgcacg aggtgtaca atttcaatc tcacgatgg 
      aaaacaaat tcggaacac atcggcaga gttacccct 
      tccaacact ttgcgcacg tattgtctg gccctcccg 
      agaatctca cagttataa acacaacct cgtcttcga 
      tctatctct actggatgg tgccccttt ccatgcgca 
      cgttctggc caggggtgc accaccgga ttctctacc 
      aatgcgaat actacgaaa agaagtggc acttcggcg 
      gcgaaatca aggaaaacc caaaaagat acgagattt 
      atttcaact tgaccaacc taaaatacg cacacgcct 
      tatgcgaca tccgcgtct cgctaagat cggccaaag 
      ctttgcctt ccgacgagt caatgtggt cgaacaacg 
      cctccatca cagtctgga cgagtagca aaataccgg 
      acgagccgc cggaaacaa acgtcggga gtttcgaag 
      tggtggcct ttggtaaca gcttgcaaa atcgggagg 
      cttacgttt actggaaac aaatcaaag aggagcaaa 
      agaacggac ccgattttc actacggca ttgctgtgg 
      aaggcgaac cggcgatac gaccggtcg aattcacca 
      acacttacg ccaagttcg aaaatttgt ccatctcga 
      ccagctaca cgttcaata tctggtcgg aaaattcaa 
      acgggactt cggcgcgca aatcgtcgg tttttatac 
      cgaaacaga cagatcgta ttaaggagc cgtggagtt 
      tcactaaag ttgagttcg gggacggtg tctacgaat 
      tgacttggg tggccccaa agttgttgg ataaccagt 
      acattacaa actacacga ttttttcct gtagcaatg 
      agcgagata ggccctacc agtgcaacg gcatgttga 
      actggaccg tggtctcga gcaacacga cttcgaaaa 
      ctatgatag ttgagaaga acaaaattt tccaatttg 
      cgatttcgg caaacacgc ataacggga gcagcggca 
      tgttgtggg cgtcctgta ccgtcatcc acaacaaaa 
      atatgggca aaatgaaaa atgtttgga taaatagcg 
      tcggctcaa ctttcatag aagtgggct ggaagctgg 
      actgcgccg atcggatcg gcagcgtcg aaggttacg 
      ttgtttatt attgcccga tcaaatcgc caatgcata 
      cggaatgca aagcgcctc aagccaatc gcaccttcg 
      acggaactt cgatgctct caagcggaa atataaccg 
      atctgaacc cttacacga catacatgt tgacggttt 
      cggtgaaaa ccaagcata cgactttta gccaacaaa 
      gcgatcctt tgtacaaca ccacgctgg aagatgctc 
      ccagcacac ctccgcgtg atgtgacta tacaaaaag 
      ttacgaatt cgtcgatta gagtcgcgt ggaagccac 
      ccgaagctc tcaatggca taattcgcg attatgttg 
      tttattgtt gcgttaaag gctcgctct gcgagaaaa 
      ttccagtca aaacggaac taaactaca cgataactg 
      gcctcaaaa gcttcgaaa actattcga tagaactcg 
      aggcttgta cgacgaagt gttcaggga agtcggaaa 
      gattacaag tgacgacta gcatgggct atccgagca 
      aaatccgta aaccaacgg tggatttgc gtaacgata 
      cttcgatta cgatcgttt ggaatgagc cagaagagc 
      ccagtgggc gcaacgatt attacgttg tacagttcc 
      gtcaaaaac acaagagtg agaataaga ctttccaat 
      acaacacaa cgaagcgca aattcgtga ttgagaatt 
      gcggcgatg gtggtaaat tcaacgcgt tttacatat 
      cagtcaagg ctgttaaca aaatagaca atcggagtt 
      acgacggcc cctggagcg acgaactcg aaaactact 
      gtgaaaccc caatcaact acatcatgt acttgctgc 
      tgttcgtcg caatcgttg ccgtggtct ccgtcggtt 
      tcttctcga ctagattat acaaccact gcaagttca 
      tgcgtgatg ttgaggtaa aactccccc ctgggttgg 
      cccccgtgg tggccgacc acaccttag tcccctgga 
      gcacggaga agcggcaag accccggcg attcgcact 
      cgccccccg acgaggagc tcctgcttg tgaagatga 
      gtgaaggga gacatttta gtggtgatt cgagtggtt 
      gcagttcag gacatgaga gtgtgacct cgtccctcg 
      aatcgggca cgcacattt cgagcagtt cggactcgg 
      ggaccgaac agccggcgg cctacagcg tgatggaag 
      cgagttcgg acacgccgt acatttccg ttactgggg 
      gctcggagg ccagctcgc cgtacgtca tgacagggg 
      atgcggtca agacgatca acccggggt acatcccct 
      tcacccagc ccccggact tcggcggcg ggctggacg 
      cgaaggggg actcgccct acatctcgg tgacggcca 
      cggacgcga ccaaaatgg ggtacaagc ccagtagtg 
      aggtttcag acgagtctt cgccatatg ttatgacgg 
      gggacgcgc ccaagacga tcaaccctg gttacatac 
      cgttcaatc agactgaac cgatgaaga attcatatg 
      tacatatta cattttagg ctaagttat gtaaatagt 
      tagcggtag gatcaggcg gatgacttg gtggtcgaa 
      actgtgcct taactatgg actgaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY