| General information | |||
| TC001867 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 493730..503940 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001867 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03261 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001867 RA
MSSGRGLIG RRRGGNTWL RTGLIIFII IFISLQINF LSYRGSSDS NSELNDSNA AILSMVPAV LHKFLTPRP RNVSSSLGL NGTTGANAA YSKWTLNIS DIKRNIAQY NLQQTVYNE DIFGPLQND SVVIVIQIH DRITYLRHL IVSLAQARG ISQALLVFS HDYYDEEIN SLVQSVDFC KVIQIFYPY SIQTHPHEF PGEDPNDCP RDIKREQAL IQKCNNALY PDLYGHYRE AKFTQTKHH WWWKANRVF NQLEITRNH TGLVVFLEE DHYVAEDFI YILKLMERT CKESCKHCN ILSLGTYLK TFNYYGDAK KKEALRAIK QQSILSAGQ VHPSWTFQV LPSLYQHSQ KVEITPWIS SKHNMGMAF NRSTWMDIV GCADYFCKY DDYNWDWSL QHISQNCLK HKFHAMVVR GPRVFHIGE CGVHHKKNN CESTAVISK VQQVLNTAK RHLYPNYLT LTYTTLLKK TKLRKGNGG WGDRRDHKL CMSMTLR
- >TC001867 RA
atgagcagc gggcgtggc ctaataggg cgtcgccga gggggaaac acatggctt cgaactgga ctaataatc ttcataata atttttatc tcccttcag ataaacttt ttaagttac cgcggttca agcgattca aattccgag ctaaacgat tcaaatgcg gcgatactg agtatggta ccggccgtg ttgcacaag ttcctaact ccaaggcct agaaacgtg agcagtagt ttgggccta aacggcacc acgggtgcc aacgcggcc tacagcaag tggacgctc aatatatcg gatatcaaa cggaacatc gcacagtac aatctccaa caaaccgtc tacaacgaa gatattttc gggcctttg caaaacgat tctgtagtt atagtaatc cagatacat gaccgcata acttatctc cgccacctt attgtgagt ctagcccaa gctcgcggc atttcccag gccctcctg gtgttctcg catgactat tacgacgag gaaataaac agtttggtc caaagcgtc gatttttgt aaagtcatc cagatattt tacccatac tcgatccaa acacatccg cacgaattc cctggcgag gatccaaac gactgtcca cgcgatatt aaacgagaa caggcccta atccagaaa tgcaacaat gccctctac cccgacctc tacggccac taccgggag gcgaaattc acccaaacg aagcaccac tggtggtgg aaggccaac cgggtgttc aaccagcta gaaatcaca aggaaccac acgggactt gtggtattt ttggaagag gatcattac gtagccgag gattttatt tacattctg aaactgatg gaacggact tgcaaggag tcttgcaaa cactgcaac atcctctcg ttgggtacc tatctaaaa acttttaat tactacggg gatgctaaa aagaaagag gctttgcga gctatcaag caacaatct attcttagc gcaggtcaa gttcatccc tcctggaca tttcaagtg ctgccttca ctctatcag cactcacag aaggtggaa attacacct tggattagc agtaagcat aacatgggg atggctttc aaccgctcc acgtggatg gatatcgta ggctgtgct gattatttt tgcaaatac gacgactac aactgggac tggtctttg caacatatt tcgcaaaac tgtttgaaa cataaattt catgcgatg gtggtacga ggtccaaga gtcttccat ataggagaa tgtggtgtg caccataag aaaaacaac tgtgaatcg acggctgta atttcaaaa gttcaacaa gtgctaaac actgctaaa agacatttg tatccgaac tatttaaca ctaacgtat acaacactt ttgaagaaa actaaatta aggaagggg aacggagga tggggggac cgaagagac cataaactt tgtatgagt atgacgctt aggtag
- >TC001867 RA
tgagttcag gctcccacg gctagatgg cttgcgttt gtcgattgt aaaataata ttctgaaaa cactgaaga caggatggg agtgagtta gtttggtgg cagtggcga gtgtttgtt gcatgtact agagagaca gctgatagt taccattaa taaattttc aaaatacgg tttctagta aatgaaatg acataaaac tggtgtaat gtgcaacga ctgtataaa atctatatt atggaccga cataaagcg aaaaggtct cgaataaat tgttgtcat gtaataaat aatcgaata atagatgaa ttggtaaat aaattaaat aatgacgag aataacaac agcgaaagg cgcaacaat aaactaacg atttaaagg tcttgtgaa tctggcatg cactaccat gagcagcgg gcgtggcct aatagggcg tcgccgagg gggaaacac atggcttcg aactggact aataatctt cataataat ttttatctc ccttcagat aaacttttt aagttaccg cggttcaag cgattcaaa ttccgagct aaacgattc aaatgcggc gatactgag tatggtacc ggccgtgtt gcacaagtt cctaactcc aaggcctag aaacgtgag cagtagttt gggcctaaa cggcaccac gggtgccaa cgcggccta cagcaagtg gacgctcaa tatatcgga tatcaaacg gaacatcgc acagtacaa tctccaaca aaccgtcta caacgaaga tattttcgg gcctttgca aaacgattc tgtagttat agtaatcca gatacatga ccgcataac ttatctccg ccaccttat tgtgagtct agcccaagc tcgcggcat ttcccaggc cctcctggt gttctcgca tgactatta cgacgagga aataaacag tttggtcca aagcgtcga tttttgtaa agtcatcca gatatttta cccatactc gatccaaac acatccgca cgaattccc tggcgagga tccaaacga ctgtccacg cgatattaa acgagaaca ggccctaat ccagaaatg caacaatgc cctctaccc cgacctcta cggccacta ccgggaggc gaaattcac ccaaacgaa gcaccactg gtggtggaa ggccaaccg ggtgttcaa ccagctaga aatcacaag gaaccacac gggacttgt ggtattttt ggaagagga tcattacgt agccgagga ttttattta cattctgaa actgatgga acggacttg caaggagtc ttgcaaaca ctgcaacat cctctcgtt gggtaccta tctaaaaac ttttaatta ctacgggga tgctaaaaa gaaagaggc tttgcgagc tatcaagca acaatctat tcttagcgc aggtcaagt tcatccctc ctggacatt tcaagtgct gccttcact ctatcagca ctcacagaa ggtggaaat tacaccttg gattagcag taagcataa catggggat ggctttcaa ccgctccac gtggatgga tatcgtagg ctgtgctga ttatttttg caaatacga cgactacaa ctgggactg gtctttgca acatatttc gcaaaactg tttgaaaca taaatttca tgcgatggt ggtacgagg tccaagagt cttccatat aggagaatg tggtgtgca ccataagaa aaacaactg tgaatcgac ggctgtaat ttcaaaagt tcaacaagt gctaaacac tgctaaaag acatttgta tccgaacta tttaacact aacgtatac aacactttt gaagaaaac taaattaag gaaggggaa cggaggatg gggggaccg aagagacca taaactttg tatgagtat gacgcttag gtagttaaa tttcgcatt tggtgatat tgtttcgat ttttgagag ccgggactg ataggtatt gttttcgta aggtgcttt agttaactt actttgttt cacctaaaa ggcgttata tttttataa cattttgtt acgtttcat cttagtcaa cattttgta tctagtctt agttattgt tagtacgtg aaaagtcag aagctagct ggtttttta ccacgcaac aaactgtgt atataaatg tgtccattt tgttgcatc ttttctaca tactcttcc tgtccaatt ctataaacc tcttgccac tttctacat acatacact aattttatc aggaagatg ctcaactat tcataagtt tttacttac atgatagca agtatttgt ttcaattct agtcatgtc tcgtagcag gaccaaaat ttttgatgt caaagctta aagttgttt tacattttc gttaaccat gcttagcgc agtataatg ttgtaactg agttcagtg tccagaaac atgtaactg cgtagcgaa taaaccata gtcagttga
