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Gene information
General information
TC001866 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 505217..509580
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001866
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
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    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03260

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001866 RA
      MVVYPYSED NLDPKITIS VLSDKIPDM PDNQLTVST 
ILSISSDIP PKELEAENV KVESLYNGK SLESIKSIA 
ISEAEAEVA AETIEKEHW YSITLQVFI PFMIAGIGT 
IGAGIVLGN VEEYEVFKE VKALYILVP SIIGLKGNL 
DMCLASRLS TQAHMGTMK SKRELFKMI VGNVGLVQV 
QAIVASCLV AIFAVAASA LVNGGFDWE HALLLSASG 
ILTATMSCF ILDFVLIAV IIITHKMKL NPDNVATPF 
AASIGDVVS LLVLSSWAS LLYSIHETH PWVMVAILG 
VYIVLVLPV WVLIVRKNK YTKKVLTHG WTPVLCALI 
ISGMGGLVL DSAVDQFTG FVVFQPIIN GIGGNLVSV 
QASRISTML HKTSLMGVI PPHTQQWVW PWKALIRGV 
LPAKSARIL VLMMIPGQA VFLFVADLI YNEGVSCVE 
PAFALTYIG VSLIQIMLL LYIAHIIIH TMWRFKMDP 
DNSAIPYLT ALGDLLGSS LLLLAFMFL REIHQEYNP 
IILANE
    CDS sequence
    • >TC001866 RA
      atggtggtg tatccgtac tcagaggac aatctcgat 
ccgaaaata acgataagt gttttgtcg gacaaaatc 
ccggatatg cccgacaac cagctaact gtttcgaca 
attttgagt atatcatca gacataccg cccaaagaa 
ttggaagca gaaaacgtt aaagttgaa tctctatat 
aacggcaag tccttggag tcgatcaaa tccatcgca 
atcagcgag gcggaggcc gaagttgcg gccgaaacc 
atcgagaaa gagcactgg tattccatc acgttacag 
gttttcatc ccgtttatg atcgcaggg atcggcaca 
atcggggcc gggatcgtt ttaggcaac gttgaggaa 
tacgaggtt ttcaaagag gtcaaagcg ctgtacatt 
ctcgtgccg tcgataatc gggctgaag ggcaacctg 
gacatgtgc ctggcctcg cgcctgtcc acccaggcc 
cacatgggc accatgaag tccaagcgc gagctgttc 
aaaatgatc gtgggcaac gtggggttg gtgcaagtc 
caggccatc gtcgcctct tgccttgtt gcgattttc 
gcggtcgcg gcctcggct ctagtcaac gggggcttt 
gattgggaa catgcgttg ttgttatca gcctcgggt 
atcttgaca gccacaatg tcatgtttc attttagat 
ttcgtacta attgctgtg ataattatt acacataag 
atgaagctc aatccggac aatgttgcc acgcccttc 
gccgctagc atcggagac gtcgtctca cttctagtc 
ctgtcgtcc tgggccagc ctgctgtac tccatccac 
gaaacgcac ccgtgggtc atggtggcg attttgggg 
gtttacatc gtcctggtg ctcccagtc tgggtgcta 
atcgtacgc aaaaataag tacacgaag aaggttcta 
acgcacggc tggactcca gttctctgc gctctcatt 
attagcggg atgggcgga ctggttctg gattctgcg 
gtggatcaa tttactggt ttcgtggtt ttccagccg 
ataatcaac ggaatcggc ggcaacctg gtctcggtg 
caggcgagt cgcatctcg acgatgctg cacaagacc 
tccctcatg ggggtcatc ccgccgcac acccagcag 
tgggtctgg ccctggaag gcgctcatc cggggcgtg 
ctgccggcg aagtcggcc cggattctg gtcctgatg 
atgattccg ggacaggcg gtcttcctc ttcgtcgcc 
gacttgatc tacaacgag ggggtgtcg tgcgtggag 
ccggccttc gccttgacg tacattgga gttagtctg 
atacagatc atgctcttg ctgtacatt gcccatatc 
atcatccac acgatgtgg aggttcaag atggacccg 
gacaactcc gccataccg tacttaaca gccctgggg 
gacctcctg ggctcgagt ctcctgctc ctggctttc 
atgttcttg agggagatt caccaagaa tacaacccg 
attattcta gccaatgaa taa
    mRNA sequence
    • >TC001866 RA
      tccgttcgt tttcccctt agatttaat ctttttgaa 
tggtcgtca aggactcat tcacgtcga aaattgagc 
gcgtttctg agaagtgaa attacaaga gacgttttg 
gacggaatt cagcctttt catacttcg gatccataa 
aaacgcaaa tcaacaatg gtggtgtat ccgtactca 
gaggacaat ctcgatccg aaaataacg ataagtgtt 
ttgtcggac aaaatcccg gatatgccc gacaaccag 
ctaactgtt tcgacaatt ttgagtata tcatcagac 
ataccgccc aaagaattg gaagcagaa aacgttaaa 
gttgaatct ctatataac ggcaagtcc ttggagtcg 
atcaaatcc atcgcaatc agcgaggcg gaggccgaa 
gttgcggcc gaaaccatc gagaaagag cactggtat 
tccatcacg ttacaggtt ttcatcccg tttatgatc 
gcagggatc ggcacaatc ggggccggg atcgtttta 
ggcaacgtt gaggaatac gaggttttc aaagaggtc 
aaagcgctg tacattctc gtgccgtcg ataatcggg 
ctgaagggc aacctggac atgtgcctg gcctcgcgc 
ctgtccacc caggcccac atgggcacc atgaagtcc 
aagcgcgag ctgttcaaa atgatcgtg ggcaacgtg 
gggttggtg caagtccag gccatcgtc gcctcttgc 
cttgttgcg attttcgcg gtcgcggcc tcggctcta 
gtcaacggg ggctttgat tgggaacat gcgttgttg 
ttatcagcc tcgggtatc ttgacagcc acaatgtca 
tgtttcatt ttagatttc gtactaatt gctgtgata 
attattaca cataagatg aagctcaat ccggacaat 
gttgccacg cccttcgcc gctagcatc ggagacgtc 
gtctcactt ctagtcctg tcgtcctgg gccagcctg 
ctgtactcc atccacgaa acgcacccg tgggtcatg 
gtggcgatt ttgggggtt tacatcgtc ctggtgctc 
ccagtctgg gtgctaatc gtacgcaaa aataagtac 
acgaagaag gttctaacg cacggctgg actccagtt 
ctctgcgct ctcattatt agcgggatg ggcggactg 
gttctggat tctgcggtg gatcaattt actggtttc 
gtggttttc cagccgata atcaacgga atcggcggc 
aacctggtc tcggtgcag gcgagtcgc atctcgacg 
atgctgcac aagacctcc ctcatgggg gtcatcccg 
ccgcacacc cagcagtgg gtctggccc tggaaggcg 
ctcatccgg ggcgtgctg ccggcgaag tcggcccgg 
attctggtc ctgatgatg attccggga caggcggtc 
ttcctcttc gtcgccgac ttgatctac aacgagggg 
gtgtcgtgc gtggagccg gccttcgcc ttgacgtac 
attggagtt agtctgata cagatcatg ctcttgctg 
tacattgcc catatcatc atccacacg atgtggagg 
ttcaagatg gacccggac aactccgcc ataccgtac 
ttaacagcc ctgggggac ctcctgggc tcgagtctc 
ctgctcctg gctttcatg ttcttgagg gagattcac 
caagaatac aacccgatt attctagcc aatgaataa 
acgcctatc cgttcgatt taactattt tacaaattt 
gatgatgta caaacttat agtattaca tttattttt 
aaatttacc gccaatgta aatttaaat ttcgtgatt 
ctattgcca gtaatttta cttgttaac gctaggaac 
aattttaat aaacataaa catgaaca
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY
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