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Gene information
General information
TC001866 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 249793..254156
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001866
    iBeetle screen iB_03260
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: < 50 embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001866 RA
      MVVYPYSED NLDPKITIS VLSDKIPDM PDNQLTVST 
      ILSISSDIP PKELEAENV KVESLYNGK SLESIKSIA 
      ISEAEAEVA AETIEKEHW YSITLQVFI PFMIAGIGT 
      IGAGIVLGN VEEYEVFKE VKALYILVP SIIGLKGNL 
      DMCLASRLS TQAHMGTMK SKRELFKMI VGNVGLVQV 
      QAIVASCLV AIFAVAASA LVNGGFDWE HALLLSASG 
      ILTATMSCF ILDFVLIAV IIITHKMKL NPDNVATPF 
      AASIGDVVS LLVLSSWAS LLYSIHETH PWVMVAILG 
      VYIVLVLPV WVLIVRKNK YTKKVLTHG WTPVLCALI 
      ISGMGGLVL DSAVDQFTG FVVFQPIIN GIGGNLVSV 
      QASRISTML HKTSLMGVI PPHTQQWVW PWKALIRGV 
      LPAKSARIL VLMMIPGQA VFLFVADLI YNEGVSCVE 
      PAFALTYIG VSLIQIMLL LYIAHIIIH TMWRFKMDP 
      DNSAIPYLT ALGDLLGSS LLLLAFMFL REIHQEYNP 
      IILANE
    CDS sequence
    • >TC001866 RA
      atggtggtg tatccgtac tcagaggac aatctcgat 
      ccgaaaata acgataagt gttttgtcg gacaaaatc 
      ccggatatg cccgacaac cagctaact gtttcgaca 
      attttgagt atatcatca gacataccg cccaaagaa 
      ttggaagca gaaaacgtt aaagttgaa tctctatat 
      aacggcaag tccttggag tcgatcaaa tccatcgca 
      atcagcgag gcggaggcc gaagttgcg gccgaaacc 
      atcgagaaa gagcactgg tattccatc acgttacag 
      gttttcatc ccgtttatg atcgcaggg atcggcaca 
      atcggggcc gggatcgtt ttaggcaac gttgaggaa 
      tacgaggtt ttcaaagag gtcaaagcg ctgtacatt 
      ctcgtgccg tcgataatc gggctgaag ggcaacctg 
      gacatgtgc ctggcctcg cgcctgtcc acccaggcc 
      cacatgggc accatgaag tccaagcgc gagctgttc 
      aaaatgatc gtgggcaac gtggggttg gtgcaagtc 
      caggccatc gtcgcctct tgccttgtt gcgattttc 
      gcggtcgcg gcctcggct ctagtcaac gggggcttt 
      gattgggaa catgcgttg ttgttatca gcctcgggt 
      atcttgaca gccacaatg tcatgtttc attttagat 
      ttcgtacta attgctgtg ataattatt acacataag 
      atgaagctc aatccggac aatgttgcc acgcccttc 
      gccgctagc atcggagac gtcgtctca cttctagtc 
      ctgtcgtcc tgggccagc ctgctgtac tccatccac 
      gaaacgcac ccgtgggtc atggtggcg attttgggg 
      gtttacatc gtcctggtg ctcccagtc tgggtgcta 
      atcgtacgc aaaaataag tacacgaag aaggttcta 
      acgcacggc tggactcca gttctctgc gctctcatt 
      attagcggg atgggcgga ctggttctg gattctgcg 
      gtggatcaa tttactggt ttcgtggtt ttccagccg 
      ataatcaac ggaatcggc ggcaacctg gtctcggtg 
      caggcgagt cgcatctcg acgatgctg cacaagacc 
      tccctcatg ggggtcatc ccgccgcac acccagcag 
      tgggtctgg ccctggaag gcgctcatc cggggcgtg 
      ctgccggcg aagtcggcc cggattctg gtcctgatg 
      atgattccg ggacaggcg gtcttcctc ttcgtcgcc 
      gacttgatc tacaacgag ggggtgtcg tgcgtggag 
      ccggccttc gccttgacg tacattgga gttagtctg 
      atacagatc atgctcttg ctgtacatt gcccatatc 
      atcatccac acgatgtgg aggttcaag atggacccg 
      gacaactcc gccataccg tacttaaca gccctgggg 
      gacctcctg ggctcgagt ctcctgctc ctggctttc 
      atgttcttg agggagatt caccaagaa tacaacccg 
      attattcta gccaatgaa taa
    mRNA sequence
    • >TC001866 RA
      tccgttcgt tttcccctt agatttaat ctttttgaa 
      tggtcgtca aggactcat tcacgtcga aaattgagc 
      gcgtttctg agaagtgaa attacaaga gacgttttg 
      gacggaatt cagcctttt catacttcg gatccataa 
      aaacgcaaa tcaacaatg gtggtgtat ccgtactca 
      gaggacaat ctcgatccg aaaataacg ataagtgtt 
      ttgtcggac aaaatcccg gatatgccc gacaaccag 
      ctaactgtt tcgacaatt ttgagtata tcatcagac 
      ataccgccc aaagaattg gaagcagaa aacgttaaa 
      gttgaatct ctatataac ggcaagtcc ttggagtcg 
      atcaaatcc atcgcaatc agcgaggcg gaggccgaa 
      gttgcggcc gaaaccatc gagaaagag cactggtat 
      tccatcacg ttacaggtt ttcatcccg tttatgatc 
      gcagggatc ggcacaatc ggggccggg atcgtttta 
      ggcaacgtt gaggaatac gaggttttc aaagaggtc 
      aaagcgctg tacattctc gtgccgtcg ataatcggg 
      ctgaagggc aacctggac atgtgcctg gcctcgcgc 
      ctgtccacc caggcccac atgggcacc atgaagtcc 
      aagcgcgag ctgttcaaa atgatcgtg ggcaacgtg 
      gggttggtg caagtccag gccatcgtc gcctcttgc 
      cttgttgcg attttcgcg gtcgcggcc tcggctcta 
      gtcaacggg ggctttgat tgggaacat gcgttgttg 
      ttatcagcc tcgggtatc ttgacagcc acaatgtca 
      tgtttcatt ttagatttc gtactaatt gctgtgata 
      attattaca cataagatg aagctcaat ccggacaat 
      gttgccacg cccttcgcc gctagcatc ggagacgtc 
      gtctcactt ctagtcctg tcgtcctgg gccagcctg 
      ctgtactcc atccacgaa acgcacccg tgggtcatg 
      gtggcgatt ttgggggtt tacatcgtc ctggtgctc 
      ccagtctgg gtgctaatc gtacgcaaa aataagtac 
      acgaagaag gttctaacg cacggctgg actccagtt 
      ctctgcgct ctcattatt agcgggatg ggcggactg 
      gttctggat tctgcggtg gatcaattt actggtttc 
      gtggttttc cagccgata atcaacgga atcggcggc 
      aacctggtc tcggtgcag gcgagtcgc atctcgacg 
      atgctgcac aagacctcc ctcatgggg gtcatcccg 
      ccgcacacc cagcagtgg gtctggccc tggaaggcg 
      ctcatccgg ggcgtgctg ccggcgaag tcggcccgg 
      attctggtc ctgatgatg attccggga caggcggtc 
      ttcctcttc gtcgccgac ttgatctac aacgagggg 
      gtgtcgtgc gtggagccg gccttcgcc ttgacgtac 
      attggagtt agtctgata cagatcatg ctcttgctg 
      tacattgcc catatcatc atccacacg atgtggagg 
      ttcaagatg gacccggac aactccgcc ataccgtac 
      ttaacagcc ctgggggac ctcctgggc tcgagtctc 
      ctgctcctg gctttcatg ttcttgagg gagattcac 
      caagaatac aacccgatt attctagcc aatgaataa 
      acgcctatc cgttcgatt taactattt tacaaattt 
      gatgatgta caaacttat agtattaca tttattttt 
      aaatttacc gccaatgta aatttaaat ttcgtgatt 
      ctattgcca gtaatttta cttgttaac gctaggaac 
      aattttaat aaacataaa catgaaca