Status
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Gene information
General information
TC031307
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001804,TC001806,TC001803,TC001805
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid897: 74456..123943
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031307
    iBeetle screen iB_03256
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    Fertility
    egg chamber decreased - Number of animals showing the phenotype: 4

    lateral oviduct structure irregular - Number of animals showing the phenotype: 4

    vitellogenic egg chamber late mostlynot present - Number of animals showing the phenotype: 4

    previtellogenic egg chamber potentiallynot present - Number of animals showing the phenotype: 4
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    iBeetle screen iB_07783
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 60.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 5 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Adult morphology
    abdominal odoriferous glands content fragmented - Number of animals showing the phenotype: 4 (13 dpi)
    Days post injection



    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031307 RA
      MSPTNPRKK FNFLSHVFF SWQIPLIIK TWKQSGQHE 
      SYFNLDEHD SEQLGNRLE FNWKKEGSQ KKFSFKRAL 
      IKTFGHELL TCNTLFLLA DVAFTLAQP MLIKLLLDE 
      LAAKQTDKV LFYVLGLLG VPILRFMSF HLFCLTITS 
      LGLKLRIAC STLIYRKIL TMRKTALEK ISLGNIVNL 
      LSNDTERFD NILLIPHIW LTPIKLIAA TLYLGLNFD 
      YSALTGIGL LVILLISQS FVYKNIASR RMNVAKKTD 
      QRIRWMNDF INGIQAIKM YTWEKLFEK FIFEARESE 
      LKEISKANY LRCCNNSFR LYLAKVSVY LCIMALVLV 
      TKIPLTSSY IFALIAIYE ILRLSTGEL LLVGVIQLA 
      EIGISVNRI ETFLLNDIA KSETLFSKQ RNKLLGVYV 
      QGLIVKRDT KIILEDVNF EALSNQLVA LVGAAGAGK 
      STFLETLLK ETEIYKGIV DLNGSVSYA PQEPWIFSS 
      SVKQNIIFC EEFDEDRYR QVIKICGLE HDLALLSHG 
      DNTLVGENG VMLSGGQKA RISLARAVY RNADIYLLD 
      DPLSAVDVY VAKQIFEQC IVDFLKGKC VVLVTHQLQ 
      FVRSVEKAY FVVNGKIVA AENTRQLDV PRPELKVAR 
      TDDDFLKHN VEKLTLESN IAGSSDALK KYLQAGGTF 
      SLTLLLSFL FILTQSLSI FNDYFLAVW VNLTETYQK 
      SSNYFYVYS IILFFLVLV TNVTFLSFV KYCMRASRR 
      LHDTLLRQV IRGSMTIFH SYTSGRIFN LFSKDIGVI 
      DEFVPLSLY LTLTQFLLI IGSIVFIFI VNYWMILPI 
      VALILMLKV CSGPFRTTI KSLRRLEAI RRNPIYNHV 
      VSTMQGITV IKSSNSRKM LEAEFSNYQ NDQASTFYL 
      FKATHFAFA FWTDIMSFI HLSCVILFY FTFTEDIYL 
      GNAGMIITH ANLLNCVIQ YFVKLWGEL DANLISVER 
      VSNFQVTQE FDEGVFEVP QNWPLTGKV VFDSVSMRY 
      SSDKNLVLN KVDIKIKGG EKVGIVGRT GAGKSSLIL 
      ALFRLFHFD GSILIDNVD TKSIPLQVL RSNISVIPQ 
      DPVLFQGSL RKNLDPFDE FSDAEVWTA LEDVQLKKI 
      VKSLDIAVN ERGSNFSVG QKQLLCLAR AILKKTKVI 
      AIDEATANV DYETDVFIQ NTIRIKFRE TTVLTIAHR 
      VNTILDSDK ILVMEEGRV VEFGPVEEL MKNVQGQFY 
      RIVKYSYSP TYKKAILKP KNQSSKESP PQKERETPR 
      PNDNYYGVV APIHYQPRR TTETEGRGQ PSTNVRTQE 
      ATDSTAVDL FCGKALIKY RCVRSRIDN WNALVTELK 
      KEFLPLDYD DLLWDEIKK RHQHKDEPT AIYVAVMES 
      LFNCLSELP TETVKVRYL ALHEILNKM EQGPSVCAM 
      TSGHVCSVP GCTNKHAPM FRFPNPKRG LDICLRWRQ 
      AVNNPKWPE LSDCRLNRI ARVCYKHFI DDDFLSGTR 
      RLTRFAVPS LNLPTGRRD STPSPRPLE LGQSSPSEV 
      SLQPKFVTP TKEFPFWLG AVGVTPRKR ETPMKGNVP 
      AKHTSEQPN AQLEVSPPC LERRIPSAG PATSKKGNR 
      HPVLSRLGI SKKPHTFGE DERRLYKFA QQLNARVKR 
      LEKSKNNLK AAMVLAKDE HVRHLIEDL TPVQQRFFE 
      SQLRNYKYK PQGRSFTFE EKADAVAIY KSSPRTYRL 
      LSKMFILPS ERTLKATLA QVSIRPGIS NHLFSVLSR 
      KIGRNKRDR ICTLIFDEI QIQPHLDYL PHEDRVIGF 
      EDDGTKRSG AVADHVAVF MLRGVFKRW KQPVAFAFC 
      KSAMKANSI VSFYKQIVA EATAVGFNI IASVCDMGS 
      NNVKAINDL LDYSKRLQT SEDDVVPDN VMRIPLRDN 
      QYHDVIPLF DPPHLLKAF RNGLLERDC QFSTHGARG 
      QQQRVVAKW DHIKRVVDI DREKNRVFR LLPKISAKH 
      VDKFKIPKM KVSIAAQVM SQRVAAVMN EFASPESGR 
      LPPDAQYTA KLLLKMDQI FDSVNGDND GLLVTIRDF 
      LSEPPEPVD RDGSGDQWH PPPPVHDDS DDLDSPSSS 
      SSDSSEGPD LVAICKQLK KALEDVKGH TRKRDLPMC 
      KLKLAPQWG RLVLPSLSV FKTKERPPH S
    CDS sequence
    • >TC031307 RA
      atgtcccca actaatccg agaaaaaaa ttcaatttt 
      ctgtcgcac gtatttttt agttggcaa ataccgcta 
      attataaaa acctggaag caaagtggt caacatgaa 
      tcgtacttc aatcttgat gaacatgac tctgaacaa 
      cttggaaat cgtctggaa ttcaactgg aaaaaagaa 
      ggttcccag aaaaaattt tcattcaaa cgtgcactt 
      atcaaaaca tttgggcat gaacttttg acttgcaat 
      actttattt ctgttagca gacgtggct tttacctta 
      gctcaaccg atgttaatc aagctactt cttgatgaa 
      cttgctgcc aaacaaacc gacaaagtg cttttttat 
      gtattggga ttattggga gtgccaatt cttcggttc 
      atgtctttt catctgttt tgtttgacg ataacttct 
      ttgggtctc aaacttcga attgcttgt agtaccctc 
      atctatcgg aaaatacta accatgaga aaaaccgct 
      ttggaaaag atttctctg ggaaacatt gtaaattta 
      ttatcaaat gatactgaa aggtttgac aacattttg 
      cttataccg catatttgg ttgaccccc atcaaacta 
      atagctgca actttgtat ctagggttg aattttgat 
      tattctgct ttgactggg attggactt ttagtaata 
      ttgttgatc tctcaaagt ttcgtatac aaaaatatc 
      gcttctaga agaatgaat gtggcaaag aaaaccgac 
      caaagaatt cgttggatg aatgacttt attaacggc 
      attcaagca attaaaatg tacacttgg gagaaattg 
      tttgaaaaa tttatattt gaggctcga gagtctgaa 
      ctgaaggag ataagcaaa gcaaattac cttcgttgt 
      tgtaacaac agttttagg ttatattta gcgaaggtt 
      tctgtttat ttgtgtatt atggcatta gtgttagtt 
      acaaaaata ccactaaca tcatcgtac atttttgca 
      ttgattgca atttacgaa attttgaga ctttccact 
      ggagaactt cttcttgtt ggtgtgata caacttgca 
      gaaatagga atttctgtc aacagaatt gaaacattt 
      cttttgaat gatatagcc aagtctgaa actcttttt 
      tccaaacaa agaaataaa ttgctaggt gtttacgtt 
      caagggtta atagttaag cgggatacg aaaattatt 
      ttagaagat gttaatttt gaagctctt tctaaccag 
      ctagtagcc ctagtaggt gctgctggt gctggaaaa 
      tcaacattt cttgaaaca ctgttaaaa gaaactgaa 
      atctataaa ggaattgtt gacttaaac ggaagtgtt 
      tcctacgct ccacaagaa ccttggatt ttttcatcc 
      tcagtgaaa cagaatatt atattttgc gaggaattt 
      gacgaggac cggtatcgc caagttatc aaaatttgt 
      ggactggag cacgattta gctcttctt tctcacggt 
      gacaacaca ttagttggt gaaaacgga gtcatgctg 
      agcggaggg cagaaagct cgcattagt ttagctaga 
      gctgtttat agaaatgcc gatatttat ctcttagat 
      gatccgtta tcggctgtt gatgtttac gtagctaaa 
      caaattttt gagcaatgt atcgtagac tttttgaaa 
      ggaaaatgc gttgtgtta gtgacccac cagttacag 
      tttgtcaga agtgtagaa aaagcctat ttcgtggtt 
      aacggaaaa atagtggct gctgaaaac acaagacag 
      cttgatgtt ccaagacca gaacttaaa gtagcaaga 
      actgacgac gattttctt aaacacaat gttgaaaaa 
      ctgacatta gaatccaat atcgcaggt tcaagtgac 
      gcattaaaa aagtatcta caagctgga gggacattt 
      tcattgact ttgttatta tcattttta tttattttg 
      acacaaagt ttgagtatt tttaatgat tattttctg 
      gcagtttgg gtcaattta actgaaaca tatcaaaag 
      tcttcaaac tacttctat gtctatagc ataattcta 
      ttttttctt gttttagta actaatgtt acttttctt 
      agtttcgtc aaatattgt atgagagct tcaagacgt 
      cttcacgat acattgctt agacaagtt ataagaggc 
      tcaatgaca attttccat tcttatact tcaggtcgt 
      atttttaat ttattttct aaagatatt ggcgtaatt 
      gacgagttc gtgccatta tcactctat ctgactttg 
      acacaattt cttctaatc attggatca attgttttt 
      atttttata gtaaattac tggatgatt ttgcctata 
      gttgcacta atattaatg ttaaaagtc tgcagtggg 
      ccatttcga acgactata aaaagttta agacgattg 
      gaagccata agaaggaat ccaatctat aatcatgtt 
      gtttcaaca atgcaagga atcacagta attaaaagt 
      tccaactcc cggaaaatg ttagaagca gaattttcc 
      aactatcaa aatgatcaa gcgtccacg ttttatctg 
      tttaaagca actcatttt gcttttgct ttttggaca 
      gatataatg tcatttatt cacctatca tgtgttatt 
      ctattctac ttcacattt actgaagat atttattta 
      ggaaatgct gggatgatt ataacacac gcaaattta 
      ttgaattgt gttatacaa tattttgtc aagctttgg 
      ggtgaatta gatgcaaat ttgatttca gtcgaacgt 
      gtgtccaat tttcaagta acacaggaa tttgacgaa 
      ggggttttt gaagtaccg caaaactgg cctctgaca 
      ggaaaagtc gtttttgat tccgtttct atgcgttac 
      tcatcagat aaaaatctc gtgcttaac aaagttgac 
      attaaaata aaaggggga gaaaaagtt ggcatagtt 
      ggtagaacc ggtgcagga aaatcatca ctaattttg 
      gcacttttc cgtttgttt catttcgac ggctcaatt 
      ctaattgat aatgtcgac accaaatca attcctctt 
      caagttctg cgttcgaac atatcggtt atccctcag 
      gatcctgtt ttatttcaa ggaagttta cgtaaaaat 
      ttagacccg ttcgatgaa tttagcgat gcagaagtg 
      tggacagct ttggaagat gtccaactg aaaaaaatt 
      gttaaaagt ctagatatt gcggtaaat gagagaggg 
      tcgaatttt agcgttgga cagaaacaa ctgttgtgt 
      ttggctcga gctattctg aaaaaaacc aaagttata 
      gcaattgat gaagctact gctaatgtt gattacgag 
      actgatgta tttattcaa aacactatt agaataaaa 
      ttcagggag acaacagtt ttgacgatt gcacatagg 
      gtgaacact attctggat tcggataaa attttggtg 
      atggaggaa ggaagagtt gtggagttt ggaccggtc 
      gaggagctt atgaagaat gtacaggga cagttttat 
      cgaattgtt aaatacagc tatagccct acgtacaaa 
      aaggccata ttgaagccc aaaaatcaa agctcaaaa 
      gaatcgccc ccacagaag gagagggag acgccacga 
      ccaaatgac aactactac ggggtggtg gcacccatc 
      cactaccag cctcgtcga actactgag actgaagga 
      agggggcag cccagcaca aatgttaga acacaagaa 
      gcgactgat agtactgca gtagatctg ttttgcggt 
      aaagctctt atcaaatac cgttgcgtt cgttcccgt 
      atcgacaat tggaacgcc ctagtaact gaacttaag 
      aaagaattt ttgccttta gactatgac gacctcttg 
      tgggacgag attaagaaa agacatcaa cataaagac 
      gaaccaaca gctatttac gttgcggtt atggaatcg 
      ttattcaat tgtctttca gaacttccc acagagacc 
      gtcaaagtt cgatacctc gcgttacat gaaattttg 
      aataaaatg gaacaagga cctagcgtc tgtgctatg 
      acctcgggg catgtttgt tcggtacca gggtgtacc 
      aacaagcat gcacccatg ttcaggttt cccaaccct 
      aagagaggg ttggacatc tgtttgaga tggaggcag 
      gccgtcaat aatccaaag tggcctgaa ttgtcggac 
      tgtcgtctc aacagaatt gcgcgtgtt tgttataaa 
      cattttata gacgacgat ttcctgtcc gggaccaga 
      cgtttaacc cgttttgct gttccttcc ctcaactta 
      ccaacaggt cgtcgggac agtacacca agtccaaga 
      ccgttggag ttggggcag tcatctcca tccgaggtg 
      tcacttcaa cccaagttt gttactccc acaaaagag 
      tttcctttt tggctagga gcagtggga gtgacaccc 
      aggaagaga gagacacct atgaaagga aatgtacct 
      gcaaaacac acatcagag caaccaaat gctcaatta 
      gaagtgtct cctccctgt cttgaacgg cgaattcct 
      tctgctggt cctgctaca agtaaaaag ggaaatcgt 
      catccagta ttatcgaga ctggggata agcaagaaa 
      ccccacaca tttggtgaa gacgaaaga agattatat 
      aaatttgcc caacagttg aacgcgaga gttaaaagg 
      ctggaaaaa tcgaagaat aacttgaaa gcagcaatg 
      gttttggct aaggatgag catgtgcgc catttaatt 
      gaagatctt actccggta caacaaaga ttttttgaa 
      agtcagttg agaaattat aagtataaa ccacaggga 
      agaagtttt acgtttgag gaaaaggct gatgccgtt 
      gccatttac aagagcagt cctcgaacg tatagactt 
      ctttctaag atgtttata ctgccgtca gaacgcaca 
      ctgaaggca actcttgca caagtttct attcgaccg 
      ggtatttca aatcatttg ttcagtgtt ctcagtcgg 
      aaaataggc cgaaataag agggatcgt atttgtact 
      ctcatcttt gatgagata caaatacaa cctcacttg 
      gactattta ccgcatgag gacagagtc ataggattt 
      gaggatgac ggtacgaaa aggtctgga gcggttgct 
      gaccatgtt gctgtcttt atgttgcga ggtgttttc 
      aaacggtgg aagcaacct gtagctttt gccttttgt 
      aaatccgca atgaaagcc aatagcatt gttagtttc 
      tacaaacaa attgttgct gaagctact gctgtgggg 
      tttaacatt atcgcctcc gtctgtgat atgggtagc 
      aataacgtt aaggcaatt aacgatcta ttagactat 
      tctaaaaga ttgcaaact tcagaagat gatgtggtg 
      ccagacaat gtgatgcgg attccactt cgtgataat 
      caatatcat gatgtgatt ccattgttt gacccacct 
      catcttctg aaggcattc cggaatggt cttttagaa 
      cgtgattgc cagttttct acccatgga gcaaggggg 
      caacagcaa cgggttgtt gcaaagtgg gatcatatt 
      aaacgggtg gttgacatt gatagggaa aagaacagg 
      gttttccgc ctgttgcca aagatttcg gctaaacat 
      gtggacaaa ttcaaaatt ccaaaaatg aaagtttca 
      atagcggca caggtaatg agtcagcga gtagcggca 
      gtgatgaat gagtttgcc agtccagaa agcgggagg 
      ttaccaccg gatgctcag tacaccgca aaacttttg 
      ttgaagatg gaccagatc tttgacagt gtcaacgga 
      gacaatgac ggcctgctg gtcacaatt agagacttt 
      ctttctgag cctccagag ccagttgat cgcgatggt 
      agtggggat cagtggcat ccaccacca ccagtacat 
      gatgatagt gatgatctt gacagcccg tcttcgagc 
      tcgtcagac tcgtcggag ggccctgac ttggttgcc 
      atttgcaaa caacttaag aaggcccta gaagacgtc 
      aaaggccat actcgtaag agagacttg cccatgtgc 
      aagctcaaa ttagcaccg caatggggg cggttggta 
      ctgccctca ctttctgtt ttcaaaacg aaggagaga 
      ccacctcac agctaa
    mRNA sequence
    • >TC031307 RA
      tggaatttg atcgtagct tagaaagta gaaattttt 
      atttgaact tcagcagtt ctagaatca tttattgaa 
      ttttaatta caacacctc gacatcagt ctccaccag 
      ttctagtgt aagcatgtc cccaactaa tccgagaaa 
      aaaattcaa ttttctgtc gcacgtatt ttttagttg 
      gcaaatacc gctaattat aaaaacctg gaagcaaag 
      tggtcaaca tgaatcgta cttcaatct tgatgaaca 
      tgactctga acaacttgg aaatcgtct ggaattcaa 
      ctggaaaaa agaaggttc ccagaaaaa attttcatt 
      caaacgtgc acttatcaa aacatttgg gcatgaact 
      tttgacttg caatacttt atttctgtt agcagacgt 
      ggcttttac cttagctca accgatgtt aatcaagct 
      acttcttga tgaacttgc tgccaaaca aaccgacaa 
      agtgctttt ttatgtatt gggattatt gggagtgcc 
      aattcttcg gttcatgtc ttttcatct gttttgttt 
      gacgataac ttctttggg tctcaaact tcgaattgc 
      ttgtagtac cctcatcta tcggaaaat actaaccat 
      gagaaaaac cgctttgga aaagatttc tctgggaaa 
      cattgtaaa tttattatc aaatgatac tgaaaggtt 
      tgacaacat tttgcttat accgcatat ttggttgac 
      ccccatcaa actaatagc tgcaacttt gtatctagg 
      gttgaattt tgattattc tgctttgac tgggattgg 
      acttttagt aatattgtt gatctctca aagtttcgt 
      atacaaaaa tatcgcttc tagaagaat gaatgtggc 
      aaagaaaac cgaccaaag aattcgttg gatgaatga 
      ctttattaa cggcattca agcaattaa aatgtacac 
      ttgggagaa attgtttga aaaatttat atttgaggc 
      tcgagagtc tgaactgaa ggagataag caaagcaaa 
      ttaccttcg ttgttgtaa caacagttt taggttata 
      tttagcgaa ggtttctgt ttatttgtg tattatggc 
      attagtgtt agttacaaa aataccact aacatcatc 
      gtacatttt tgcattgat tgcaattta cgaaatttt 
      gagactttc cactggaga acttcttct tgttggtgt 
      gatacaact tgcagaaat aggaatttc tgtcaacag 
      aattgaaac atttctttt gaatgatat agccaagtc 
      tgaaactct tttttccaa acaaagaaa taaattgct 
      aggtgttta cgttcaagg gttaatagt taagcggga 
      tacgaaaat tattttaga agatgttaa ttttgaagc 
      tctttctaa ccagctagt agccctagt aggtgctgc 
      tggtgctgg aaaatcaac atttcttga aacactgtt 
      aaaagaaac tgaaatcta taaaggaat tgttgactt 
      aaacggaag tgtttccta cgctccaca agaaccttg 
      gattttttc atcctcagt gaaacagaa tattatatt 
      ttgcgagga atttgacga ggaccggta tcgccaagt 
      tatcaaaat ttgtggact ggagcacga tttagctct 
      tctttctca cggtgacaa cacattagt tggtgaaaa 
      cggagtcat gctgagcgg agggcagaa agctcgcat 
      tagtttagc tagagctgt ttatagaaa tgccgatat 
      ttatctctt agatgatcc gttatcggc tgttgatgt 
      ttacgtagc taaacaaat ttttgagca atgtatcgt 
      agacttttt gaaaggaaa atgcgttgt gttagtgac 
      ccaccagtt acagtttgt cagaagtgt agaaaaagc 
      ctatttcgt ggttaacgg aaaaatagt ggctgctga 
      aaacacaag acagcttga tgttccaag accagaact 
      taaagtagc aagaactga cgacgattt tcttaaaca 
      caatgttga aaaactgac attagaatc caatatcgc 
      aggttcaag tgacgcatt aaaaaagta tctacaagc 
      tggagggac attttcatt gactttgtt attatcatt 
      tttatttat tttgacaca aagtttgag tatttttaa 
      tgattattt tctggcagt ttgggtcaa tttaactga 
      aacatatca aaagtcttc aaactactt ctatgtcta 
      tagcataat tctattttt tcttgtttt agtaactaa 
      tgttacttt tcttagttt cgtcaaata ttgtatgag 
      agcttcaag acgtcttca cgatacatt gcttagaca 
      agttataag aggctcaat gacaatttt ccattctta 
      tacttcagg tcgtatttt taatttatt ttctaaaga 
      tattggcgt aattgacga gttcgtgcc attatcact 
      ctatctgac tttgacaca atttcttct aatcattgg 
      atcaattgt ttttatttt tatagtaaa ttactggat 
      gattttgcc tatagttgc actaatatt aatgttaaa 
      agtctgcag tgggccatt tcgaacgac tataaaaag 
      tttaagacg attggaagc cataagaag gaatccaat 
      ctataatca tgttgtttc aacaatgca aggaatcac 
      agtaattaa aagttccaa ctcccggaa aatgttaga 
      agcagaatt ttccaacta tcaaaatga tcaagcgtc 
      cacgtttta tctgtttaa agcaactca ttttgcttt 
      tgctttttg gacagatat aatgtcatt tattcacct 
      atcatgtgt tattctatt ctacttcac atttactga 
      agatattta tttaggaaa tgctgggat gattataac 
      acacgcaaa tttattgaa ttgtgttat acaatattt 
      tgtcaagct ttggggtga attagatgc aaatttgat 
      ttcagtcga acgtgtgtc caattttca agtaacaca 
      ggaatttga cgaaggggt ttttgaagt accgcaaaa 
      ctggcctct gacaggaaa agtcgtttt tgattccgt 
      ttctatgcg ttactcatc agataaaaa tctcgtgct 
      taacaaagt tgacattaa aataaaagg gggagaaaa 
      agttggcat agttggtag aaccggtgc aggaaaatc 
      atcactaat tttggcact tttccgttt gtttcattt 
      cgacggctc aattctaat tgataatgt cgacaccaa 
      atcaattcc tcttcaagt tctgcgttc gaacatatc 
      ggttatccc tcaggatcc tgttttatt tcaaggaag 
      tttacgtaa aaatttaga cccgttcga tgaatttag 
      cgatgcaga agtgtggac agctttgga agatgtcca 
      actgaaaaa aattgttaa aagtctaga tattgcggt 
      aaatgagag agggtcgaa ttttagcgt tggacagaa 
      acaactgtt gtgtttggc tcgagctat tctgaaaaa 
      aaccaaagt tatagcaat tgatgaagc tactgctaa 
      tgttgatta cgagactga tgtatttat tcaaaacac 
      tattagaat aaaattcag ggagacaac agttttgac 
      gattgcaca tagggtgaa cactattct ggattcgga 
      taaaatttt ggtgatgga ggaaggaag agttgtgga 
      gtttggacc ggtcgagga gcttatgaa gaatgtaca 
      gggacagtt ttatcgaat tgttaaata cagctatag 
      ccctacgta caaaaaggc catattgaa gcccaaaaa 
      tcaaagctc aaaagaatc gcccccaca gaaggagag 
      ggagacgcc acgaccaaa tgacaacta ctacggggt 
      ggtggcacc catccacta ccagcctcg tcgaactac 
      tgagactga aggaagggg gcagcccag cacaaatgt 
      tagaacaca agaagcgac tgatagtac tgcagtaga 
      tctgttttg cggtaaagc tcttatcaa ataccgttg 
      cgttcgttc ccgtatcga caattggaa cgccctagt 
      aactgaact taagaaaga atttttgcc tttagacta 
      tgacgacct cttgtggga cgagattaa gaaaagaca 
      tcaacataa agacgaacc aacagctat ttacgttgc 
      ggttatgga atcgttatt caattgtct ttcagaact 
      tcccacaga gaccgtcaa agttcgata cctcgcgtt 
      acatgaaat tttgaataa aatggaaca aggacctag 
      cgtctgtgc tatgacctc ggggcatgt ttgttcggt 
      accagggtg taccaacaa gcatgcacc catgttcag 
      gtttcccaa ccctaagag agggttgga catctgttt 
      gagatggag gcaggccgt caataatcc aaagtggcc 
      tgaattgtc ggactgtcg tctcaacag aattgcgcg 
      tgtttgtta taaacattt tatagacga cgatttcct 
      gtccgggac cagacgttt aacccgttt tgctgttcc 
      ttccctcaa cttaccaac aggtcgtcg ggacagtac 
      accaagtcc aagaccgtt ggagttggg gcagtcatc 
      tccatccga ggtgtcact tcaacccaa gtttgttac 
      tcccacaaa agagtttcc tttttggct aggagcagt 
      gggagtgac acccaggaa gagagagac acctatgaa 
      aggaaatgt acctgcaaa acacacatc agagcaacc 
      aaatgctca attagaagt gtctcctcc ctgtcttga 
      acggcgaat tccttctgc tggtcctgc tacaagtaa 
      aaagggaaa tcgtcatcc agtattatc gagactggg 
      gataagcaa gaaacccca cacatttgg tgaagacga 
      aagaagatt atataaatt tgcccaaca gttgaacgc 
      gagagttaa aaggctgga aaaatcgaa gaataactt 
      gaaagcagc aatggtttt ggctaagga tgagcatgt 
      gcgccattt aattgaaga tcttactcc ggtacaaca 
      aagattttt tgaaagtca gttgagaaa ttataagta 
      taaaccaca gggaagaag ttttacgtt tgaggaaaa 
      ggctgatgc cgttgccat ttacaagag cagtcctcg 
      aacgtatag acttctttc taagatgtt tatactgcc 
      gtcagaacg cacactgaa ggcaactct tgcacaagt 
      ttctattcg accgggtat ttcaaatca tttgttcag 
      tgttctcag tcggaaaat aggccgaaa taagaggga 
      tcgtatttg tactctcat ctttgatga gatacaaat 
      acaacctca cttggacta tttaccgca tgaggacag 
      agtcatagg atttgagga tgacggtac gaaaaggtc 
      tggagcggt tgctgacca tgttgctgt ctttatgtt 
      gcgaggtgt tttcaaacg gtggaagca acctgtagc 
      ttttgcctt ttgtaaatc cgcaatgaa agccaatag 
      cattgttag tttctacaa acaaattgt tgctgaagc 
      tactgctgt ggggtttaa cattatcgc ctccgtctg 
      tgatatggg tagcaataa cgttaaggc aattaacga 
      tctattaga ctattctaa aagattgca aacttcaga 
      agatgatgt ggtgccaga caatgtgat gcggattcc 
      acttcgtga taatcaata tcatgatgt gattccatt 
      gtttgaccc acctcatct tctgaaggc attccggaa 
      tggtctttt agaacgtga ttgccagtt ttctaccca 
      tggagcaag ggggcaaca gcaacgggt tgttgcaaa 
      gtgggatca tattaaacg ggtggttga cattgatag 
      ggaaaagaa cagggtttt ccgcctgtt gccaaagat 
      ttcggctaa acatgtgga caaattcaa aattccaaa 
      aatgaaagt ttcaatagc ggcacaggt aatgagtca 
      gcgagtagc ggcagtgat gaatgagtt tgccagtcc 
      agaaagcgg gaggttacc accggatgc tcagtacac 
      cgcaaaact tttgttgaa gatggacca gatctttga 
      cagtgtcaa cggagacaa tgacggcct gctggtcac 
      aattagaga ctttctttc tgagcctcc agagccagt 
      tgatcgcga tggtagtgg ggatcagtg gcatccacc 
      accaccagt acatgatga tagtgatga tcttgacag 
      cccgtcttc gagctcgtc agactcgtc ggagggccc 
      tgacttggt tgccatttg caaacaact taagaaggc 
      cctagaaga cgtcaaagg ccatactcg taagagaga 
      cttgcccat gtgcaagct caaattagc accgcaatg 
      ggggcggtt ggtactgcc ctcactttc tgttttcaa 
      aacgaagga gagaccacc tcacagcta atcgagtta 
      ctgggtgac gcggggaaa ctcctggct tccgttcac 
      tttgccgag tcccaatct cgtaaaatc ttggcctca 
      gcaggcatt aataaatta acattgacg gc