Status
Please wait ...
Gene information
General information
TC031577 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7857039..7865648
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031577
    iBeetle screen iB_03252

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    head not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    thorax not present - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen seta pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    abdomen segment partially fused - Penetrance (percentage of animals/eggs on slide): less than 30%

    abdomen segment number decreased - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    abdomen circumference irregular -

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031577 RA
      MDCICSKPR VIHCKIILL DEQELIHEI EDSCTGQAL 
      LDVVFRNLN LLETAYFGL RYLDQTGQT HWLDPAKTL 
      SKQIKGPDP FTFYFGVKF YAADPCKLL EEITRYQFF 
      LQVKQDILQ ERLLVPFEL AAELGAYVV QSELGDYDP 
      RRHSKGYVS EFRFLPNQS IDLETRIAE IHKTLVGQL 
      PSVAELNYL EKVKWLEMY GVDLHPVLG EDSVEYFLG 
      LTPSGIIVL RNKTTVGNY YWPRITKIY FKGRYFMLR 
      VIDKNNDES TYGFETPSK SGCKHLWKC CVEHHAFFR 
      LVQVTPTTP DIFALGSRF RYSGRTEKQ AVRDAQMKV 
      RTPPAFSRT PSRRYQRRV IEGIAEHLP TDETYGRSQ 
      YQPQPQETK FISVPQPAQ NFGGSHPSF NANSDETKR 
      ADSPRSIKS APWSQPHSK GLYNNSVPI SPRSVRSAG 
      PRYRSSSLD SQSSNDSRS CKKRKHRSK LTSDNESEL 
      SKSSNRSHR KHRRRRSKS RRDSESEPE SERKYSSKL 
      RQVDHQYEL VNSDYQWRE VQKKLYNES TGNSIQQAN 
      VISSRRSCQ QIYDGKNEC EMIYPNKVR KHRKHRSRS 
      RSPSEPKSW LSDELKKHL EFDLIDTTG MSDSQLKEI 
      PYTVVQTNK PKQIKLKPQ WNKSIHDDK KCNTPPPPY 
      SPHITETHR IRNVCLGHY NLNEDLSPQ LTYNGNDNS 
      ADAVLPRMF SSLEVADFP NNFSHHPKS YNPGLERRQ 
      ENSDHLGIS HEHTDSGLG TEQDCIYNS EKSNERNGF 
      NNVEDYLLV QRDYQMLLN TMRYHKQIQ KEAAPVKLL 
      QR
    • >TC031577 RB
      MDCICSKPR VIHCKIILL DEQELIHEI EDSCTGQAL 
      LDVVFRNLN LLETAYFGL RYLDQTGQT HWLDPAKTL 
      SKQIKGPDP FTFYFGVKF YAADPCKLL EEITRYQFF 
      LQVKQDILQ ERLLVPFEL AAELGAYVV QSELGDYDP 
      RRHSKGYVS EFRFLPNQS IDLETRIAE IHKTLVGQL 
      PSVAELNYL EKVKWLEMY GVDLHPVLG EDSVEYFLG 
      LTPSGIIVL RNKTTVGNY YWPRITKIY FKGRYFMLR 
      VIDKNNDES TYGFETPSK SGCKHLWKC CVEHHAFFR 
      LVQVTPTTP DIFALGSRF RYSGRTEKQ AVRDAQMKV 
      RTPPAFSRT PSRRYQRRV IEGIAEHLP TDETYGRSQ 
      YQPQPQETK FISVPQPAQ NFGGSHPSF NANSDETKR 
      ADSPRSIKS APWSQPHSK GLYNNSVPI SPRSVRSAG 
      PRYRSSSLD SQSSNDSRS CKKRKHRSK LTSDNESEL 
      SKSSNRSHR KHRRRRSKS RRDSESEPE SERKYSSKL 
      RQVDHQYEL VNSDYQWRE VQKKLYNES TGNSIQQAN 
      VISSRRSCQ QIYDGKNEC EMIYPNKVR KHRKHRSRS 
      RSPSEPKSW LSDELKKHL EFDLIDTTG MSDSQLKEI 
      PYTVVQTNK PKQIKLKPQ WNKSIHDDK KCNTPPPPY 
      SPHITETHR IRNVCLGHY NLNEDLSPQ LTYNGNDNS 
      ADAVLPRMF SSLEVADFP NNFSHHPKS YNPGLERRQ 
      ENSDHLGIS HEHTDSGLG TEQDCIYNS EKSNERNGF 
      NNVEDYLLV SGPERLSNV TEHNALPQA NSEGGGASE 
      ATAAVDLSE MSTEL
    CDS sequence
    • >TC031577 RA
      atggactgt atttgttcg aaaccgaga gtaatccac 
      tgcaaaata attcttttg gatgaacaa gagctaatt 
      cacgagatc gaagattct tgcaccgga caagcttta 
      ctcgacgtc gtttttcgc aatttaaat ctactcgaa 
      acggcctat ttcggcttg cgatattta gaccaaacc 
      ggacaaact cactggctc gacccagcc aaaactcta 
      agcaaacaa attaaagga cctgatcct ttcactttc 
      tattttgga gtcaaattt tacgccgcc gatccctgc 
      aaactcctc gaagaaatc acaagatat cagtttttt 
      ttgcaagtg aagcaggac attttgcaa gagcgcttg 
      ctggtgccc tttgagttg gcggccgag ttgggggcg 
      tatgtggtg caatcggag cttggtgac tatgacccc 
      cggcgtcac agcaaaggc tacgtatcg gaatttcgt 
      ttcctgcct aatcagagc atagatctt gaaacgcga 
      attgctgaa attcataaa actctagta ggtcagtta 
      ccatccgta gcggaacta aactacctc gaaaaggtg 
      aaatggctg gagatgtac ggcgttgat ctgcacccc 
      gttctgggg gaggacagt gtggagtat ttcctggga 
      ttaacgccg agcgggata atcgtgctg aggaacaaa 
      acaacggtg ggcaattat tactggcca aggattaca 
      aaaatttat ttcaaaggc cgatatttc atgttaaga 
      gtcatcgat aaaaacaac gatgaaagc acgtacgga 
      tttgaaaca ccatcaaaa tcgggatgc aaacatctc 
      tggaaatgt tgcgtcgaa caccacgcc tttttcagg 
      ctggttcag gtgacccca actacgccc gacattttc 
      gcactgggt tcaaggttc agatacagc ggccggacg 
      gagaagcaa gccgtcaga gacgcccag atgaaggtg 
      cggaccccc ccggccttc tccaggaca ccttcgcga 
      cgataccaa agacgagtt attgaggga atagctgaa 
      cacttgcca actgatgaa acttacgga cgatctcaa 
      tatcaacca cagccacag gaaaccaag tttatttcc 
      gtgcctcag cccgcacaa aattttgga ggttcacac 
      ccgtctttc aatgcaaat tccgacgag acgaagcga 
      gcagactcg cccaggagc atcaaaagt gccccctgg 
      tcgcagccg cattccaaa ggactttac aataattcc 
      gtcccgatc agcccgcga tcggtgcgg tcggcggga 
      cccaggtac aggtcctcc tcactcgat agccaaagt 
      tccaacgat tcgcgctct tgcaaaaaa cgcaaacat 
      cggagtaaa ttaacttca gacaatgag agtgaattg 
      tcgaaaagc tccaatcga tcacacagg aaacacagg 
      agacgcaga tccaaaagt cgacgcgac tcggagtca 
      gagcccgag tccgagcgg aaatactcc agcaaatta 
      aggcaagtg gaccaccag tacgagttg gtgaattcg 
      gactaccag tggcgcgag gtccagaaa aaattgtac 
      aacgaaagc accggcaac agtattcag caggcgaat 
      gtgataagc agtcgtcgt tcgtgtcag caaatttac 
      gacggcaaa aatgagtgc gagatgatt tatccgaat 
      aaagtgcgt aaacaccgc aaacaccga tcgcgctcc 
      cgctctccg tccgagccc aagtcctgg ctttccgac 
      gaattgaaa aagcattta gaattcgat cttatcgat 
      actacgggt atgtccgac tctcagctg aaggaaatt 
      ccttatacg gtagtccaa acgaataaa cctaaacag 
      atcaagctg aaaccacaa tggaataag agcattcac 
      gacgataaa aaatgcaac actccgcct ccgccatac 
      tcacctcac atcacggaa acgcataga atacgaaat 
      gtttgccta ggtcattat aatttaaac gaagattta 
      tcgccacaa ttgacttat aacggaaat gataacagc 
      gcggacgcg gttctgcca aggatgttt agctcgctg 
      gaagtggcc gattttccg aataatttc agccaccac 
      cccaagagc tacaatccc gggcttgag cggcgacag 
      gaaaactcc gatcaccta ggaataagt catgaacac 
      acggattcg gggctcggg accgagcag gactgcatc 
      tacaacagc gaaaagtcc aacgaacgg aacggattt 
      aacaacgtc gaggattat ttactagtc cagagagat 
      tatcaaatg ttactgaac acaatgcgt taccacaag 
      caaattcag aaggaggcg gcgccagtg aagctactg 
      cagcggtag
    • >TC031577 RB
      atggactgt atttgttcg aaaccgaga gtaatccac 
      tgcaaaata attcttttg gatgaacaa gagctaatt 
      cacgagatc gaagattct tgcaccgga caagcttta 
      ctcgacgtc gtttttcgc aatttaaat ctactcgaa 
      acggcctat ttcggcttg cgatattta gaccaaacc 
      ggacaaact cactggctc gacccagcc aaaactcta 
      agcaaacaa attaaagga cctgatcct ttcactttc 
      tattttgga gtcaaattt tacgccgcc gatccctgc 
      aaactcctc gaagaaatc acaagatat cagtttttt 
      ttgcaagtg aagcaggac attttgcaa gagcgcttg 
      ctggtgccc tttgagttg gcggccgag ttgggggcg 
      tatgtggtg caatcggag cttggtgac tatgacccc 
      cggcgtcac agcaaaggc tacgtatcg gaatttcgt 
      ttcctgcct aatcagagc atagatctt gaaacgcga 
      attgctgaa attcataaa actctagta ggtcagtta 
      ccatccgta gcggaacta aactacctc gaaaaggtg 
      aaatggctg gagatgtac ggcgttgat ctgcacccc 
      gttctgggg gaggacagt gtggagtat ttcctggga 
      ttaacgccg agcgggata atcgtgctg aggaacaaa 
      acaacggtg ggcaattat tactggcca aggattaca 
      aaaatttat ttcaaaggc cgatatttc atgttaaga 
      gtcatcgat aaaaacaac gatgaaagc acgtacgga 
      tttgaaaca ccatcaaaa tcgggatgc aaacatctc 
      tggaaatgt tgcgtcgaa caccacgcc tttttcagg 
      ctggttcag gtgacccca actacgccc gacattttc 
      gcactgggt tcaaggttc agatacagc ggccggacg 
      gagaagcaa gccgtcaga gacgcccag atgaaggtg 
      cggaccccc ccggccttc tccaggaca ccttcgcga 
      cgataccaa agacgagtt attgaggga atagctgaa 
      cacttgcca actgatgaa acttacgga cgatctcaa 
      tatcaacca cagccacag gaaaccaag tttatttcc 
      gtgcctcag cccgcacaa aattttgga ggttcacac 
      ccgtctttc aatgcaaat tccgacgag acgaagcga 
      gcagactcg cccaggagc atcaaaagt gccccctgg 
      tcgcagccg cattccaaa ggactttac aataattcc 
      gtcccgatc agcccgcga tcggtgcgg tcggcggga 
      cccaggtac aggtcctcc tcactcgat agccaaagt 
      tccaacgat tcgcgctct tgcaaaaaa cgcaaacat 
      cggagtaaa ttaacttca gacaatgag agtgaattg 
      tcgaaaagc tccaatcga tcacacagg aaacacagg 
      agacgcaga tccaaaagt cgacgcgac tcggagtca 
      gagcccgag tccgagcgg aaatactcc agcaaatta 
      aggcaagtg gaccaccag tacgagttg gtgaattcg 
      gactaccag tggcgcgag gtccagaaa aaattgtac 
      aacgaaagc accggcaac agtattcag caggcgaat 
      gtgataagc agtcgtcgt tcgtgtcag caaatttac 
      gacggcaaa aatgagtgc gagatgatt tatccgaat 
      aaagtgcgt aaacaccgc aaacaccga tcgcgctcc 
      cgctctccg tccgagccc aagtcctgg ctttccgac 
      gaattgaaa aagcattta gaattcgat cttatcgat 
      actacgggt atgtccgac tctcagctg aaggaaatt 
      ccttatacg gtagtccaa acgaataaa cctaaacag 
      atcaagctg aaaccacaa tggaataag agcattcac 
      gacgataaa aaatgcaac actccgcct ccgccatac 
      tcacctcac atcacggaa acgcataga atacgaaat 
      gtttgccta ggtcattat aatttaaac gaagattta 
      tcgccacaa ttgacttat aacggaaat gataacagc 
      gcggacgcg gttctgcca aggatgttt agctcgctg 
      gaagtggcc gattttccg aataatttc agccaccac 
      cccaagagc tacaatccc gggcttgag cggcgacag 
      gaaaactcc gatcaccta ggaataagt catgaacac 
      acggattcg gggctcggg accgagcag gactgcatc 
      tacaacagc gaaaagtcc aacgaacgg aacggattt 
      aacaacgtc gaggattat ttactagtt tcaggtcca 
      gagagatta tcaaatgtt actgaacac aatgcgtta 
      ccacaagca aattcagaa ggaggcggc gccagtgaa 
      gctactgca gcggtagat ttgtcggaa atgagcacc 
      gagctgtaa
    mRNA sequence
    • >TC031577 RA
      agtaaatta gacccgttt tcccacctc ttcacttgc 
      caagacgat tcgtaaacc gaacagcat gtaagcaat 
      ttaccgtgt caatttcca tgggtcggt tgttctctt 
      ctgtagtta gaagcaggt ctctgtggt gtgctgttc 
      ttacctgta ccttgattt aaaactgaa cttcaaagt 
      tgaatggcc taggatgta catcgctat ttaactgta 
      tattgctag tgcagtgga gtgatgttg agttatatt 
      ttaataacg atgattaat tgattttat ttattacga 
      gttgaggat tattttcac ggccactcc aggttttta 
      cttagtatg gactgtatt tgttcgaaa ccgagagta 
      atccactgc aaaataatt cttttggat gaacaagag 
      ctaattcac gagatcgaa gattcttgc accggacaa 
      gctttactc gacgtcgtt tttcgcaat ttaaatcta 
      ctcgaaacg gcctatttc ggcttgcga tatttagac 
      caaaccgga caaactcac tggctcgac ccagccaaa 
      actctaagc aaacaaatt aaaggacct gatcctttc 
      actttctat tttggagtc aaattttac gccgccgat 
      ccctgcaaa ctcctcgaa gaaatcaca agatatcag 
      ttttttttg caagtgaag caggacatt ttgcaagag 
      cgcttgctg gtgcccttt gagttggcg gccgagttg 
      ggggcgtat gtggtgcaa tcggagctt ggtgactat 
      gacccccgg cgtcacagc aaaggctac gtatcggaa 
      tttcgtttc ctgcctaat cagagcata gatcttgaa 
      acgcgaatt gctgaaatt cataaaact ctagtaggt 
      cagttacca tccgtagcg gaactaaac tacctcgaa 
      aaggtgaaa tggctggag atgtacggc gttgatctg 
      caccccgtt ctgggggag gacagtgtg gagtatttc 
      ctgggatta acgccgagc gggataatc gtgctgagg 
      aacaaaaca acggtgggc aattattac tggccaagg 
      attacaaaa atttatttc aaaggccga tatttcatg 
      ttaagagtc atcgataaa aacaacgat gaaagcacg 
      tacggattt gaaacacca tcaaaatcg ggatgcaaa 
      catctctgg aaatgttgc gtcgaacac cacgccttt 
      ttcaggctg gttcaggtg accccaact acgcccgac 
      attttcgca ctgggttca aggttcaga tacagcggc 
      cggacggag aagcaagcc gtcagagac gcccagatg 
      aaggtgcgg acccccccg gccttctcc aggacacct 
      tcgcgacga taccaaaga cgagttatt gagggaata 
      gctgaacac ttgccaact gatgaaact tacggacga 
      tctcaatat caaccacag ccacaggaa accaagttt 
      atttccgtg cctcagccc gcacaaaat tttggaggt 
      tcacacccg tctttcaat gcaaattcc gacgagacg 
      aagcgagca gactcgccc aggagcatc aaaagtgcc 
      ccctggtcg cagccgcat tccaaagga ctttacaat 
      aattccgtc ccgatcagc ccgcgatcg gtgcggtcg 
      gcgggaccc aggtacagg tcctcctca ctcgatagc 
      caaagttcc aacgattcg cgctcttgc aaaaaacgc 
      aaacatcgg agtaaatta acttcagac aatgagagt 
      gaattgtcg aaaagctcc aatcgatca cacaggaaa 
      cacaggaga cgcagatcc aaaagtcga cgcgactcg 
      gagtcagag cccgagtcc gagcggaaa tactccagc 
      aaattaagg caagtggac caccagtac gagttggtg 
      aattcggac taccagtgg cgcgaggtc cagaaaaaa 
      ttgtacaac gaaagcacc ggcaacagt attcagcag 
      gcgaatgtg ataagcagt cgtcgttcg tgtcagcaa 
      atttacgac ggcaaaaat gagtgcgag atgatttat 
      ccgaataaa gtgcgtaaa caccgcaaa caccgatcg 
      cgctcccgc tctccgtcc gagcccaag tcctggctt 
      tccgacgaa ttgaaaaag catttagaa ttcgatctt 
      atcgatact acgggtatg tccgactct cagctgaag 
      gaaattcct tatacggta gtccaaacg aataaacct 
      aaacagatc aagctgaaa ccacaatgg aataagagc 
      attcacgac gataaaaaa tgcaacact ccgcctccg 
      ccatactca cctcacatc acggaaacg catagaata 
      cgaaatgtt tgcctaggt cattataat ttaaacgaa 
      gatttatcg ccacaattg acttataac ggaaatgat 
      aacagcgcg gacgcggtt ctgccaagg atgtttagc 
      tcgctggaa gtggccgat tttccgaat aatttcagc 
      caccacccc aagagctac aatcccggg cttgagcgg 
      cgacaggaa aactccgat cacctagga ataagtcat 
      gaacacacg gattcgggg ctcgggacc gagcaggac 
      tgcatctac aacagcgaa aagtccaac gaacggaac 
      ggatttaac aacgtcgag gattattta ctagtccag 
      agagattat caaatgtta ctgaacaca atgcgttac 
      cacaagcaa attcagaag gaggcggcg ccagtgaag 
      ctactgcag cggtagatt tgtcggaaa tgagcaccg 
      agctgtaac tcatactct agcgatcca aataatttc 
      atgtaattt ta
    • >TC031577 RB
      agtaaatta gacccgttt tcccacctc ttcacttgc 
      caagacgat tcgtaaacc gaacagcat gtaagcaat 
      ttaccgtgt caatttcca tgggtcggt tgttctctt 
      ctgtagtta gaagcaggt ctctgtggt gtgctgttc 
      ttacctgta ccttgattt aaaactgaa cttcaaagt 
      tgaatggcc taggatgta catcgctat ttaactgta 
      tattgctag tgcagtgga gtgatgttg agttatatt 
      ttaataacg atgattaat tgattttat ttattacga 
      gttgaggat tattttcac ggccactcc aggttttta 
      cttagtatg gactgtatt tgttcgaaa ccgagagta 
      atccactgc aaaataatt cttttggat gaacaagag 
      ctaattcac gagatcgaa gattcttgc accggacaa 
      gctttactc gacgtcgtt tttcgcaat ttaaatcta 
      ctcgaaacg gcctatttc ggcttgcga tatttagac 
      caaaccgga caaactcac tggctcgac ccagccaaa 
      actctaagc aaacaaatt aaaggacct gatcctttc 
      actttctat tttggagtc aaattttac gccgccgat 
      ccctgcaaa ctcctcgaa gaaatcaca agatatcag 
      ttttttttg caagtgaag caggacatt ttgcaagag 
      cgcttgctg gtgcccttt gagttggcg gccgagttg 
      ggggcgtat gtggtgcaa tcggagctt ggtgactat 
      gacccccgg cgtcacagc aaaggctac gtatcggaa 
      tttcgtttc ctgcctaat cagagcata gatcttgaa 
      acgcgaatt gctgaaatt cataaaact ctagtaggt 
      cagttacca tccgtagcg gaactaaac tacctcgaa 
      aaggtgaaa tggctggag atgtacggc gttgatctg 
      caccccgtt ctgggggag gacagtgtg gagtatttc 
      ctgggatta acgccgagc gggataatc gtgctgagg 
      aacaaaaca acggtgggc aattattac tggccaagg 
      attacaaaa atttatttc aaaggccga tatttcatg 
      ttaagagtc atcgataaa aacaacgat gaaagcacg 
      tacggattt gaaacacca tcaaaatcg ggatgcaaa 
      catctctgg aaatgttgc gtcgaacac cacgccttt 
      ttcaggctg gttcaggtg accccaact acgcccgac 
      attttcgca ctgggttca aggttcaga tacagcggc 
      cggacggag aagcaagcc gtcagagac gcccagatg 
      aaggtgcgg acccccccg gccttctcc aggacacct 
      tcgcgacga taccaaaga cgagttatt gagggaata 
      gctgaacac ttgccaact gatgaaact tacggacga 
      tctcaatat caaccacag ccacaggaa accaagttt 
      atttccgtg cctcagccc gcacaaaat tttggaggt 
      tcacacccg tctttcaat gcaaattcc gacgagacg 
      aagcgagca gactcgccc aggagcatc aaaagtgcc 
      ccctggtcg cagccgcat tccaaagga ctttacaat 
      aattccgtc ccgatcagc ccgcgatcg gtgcggtcg 
      gcgggaccc aggtacagg tcctcctca ctcgatagc 
      caaagttcc aacgattcg cgctcttgc aaaaaacgc 
      aaacatcgg agtaaatta acttcagac aatgagagt 
      gaattgtcg aaaagctcc aatcgatca cacaggaaa 
      cacaggaga cgcagatcc aaaagtcga cgcgactcg 
      gagtcagag cccgagtcc gagcggaaa tactccagc 
      aaattaagg caagtggac caccagtac gagttggtg 
      aattcggac taccagtgg cgcgaggtc cagaaaaaa 
      ttgtacaac gaaagcacc ggcaacagt attcagcag 
      gcgaatgtg ataagcagt cgtcgttcg tgtcagcaa 
      atttacgac ggcaaaaat gagtgcgag atgatttat 
      ccgaataaa gtgcgtaaa caccgcaaa caccgatcg 
      cgctcccgc tctccgtcc gagcccaag tcctggctt 
      tccgacgaa ttgaaaaag catttagaa ttcgatctt 
      atcgatact acgggtatg tccgactct cagctgaag 
      gaaattcct tatacggta gtccaaacg aataaacct 
      aaacagatc aagctgaaa ccacaatgg aataagagc 
      attcacgac gataaaaaa tgcaacact ccgcctccg 
      ccatactca cctcacatc acggaaacg catagaata 
      cgaaatgtt tgcctaggt cattataat ttaaacgaa 
      gatttatcg ccacaattg acttataac ggaaatgat 
      aacagcgcg gacgcggtt ctgccaagg atgtttagc 
      tcgctggaa gtggccgat tttccgaat aatttcagc 
      caccacccc aagagctac aatcccggg cttgagcgg 
      cgacaggaa aactccgat cacctagga ataagtcat 
      gaacacacg gattcgggg ctcgggacc gagcaggac 
      tgcatctac aacagcgaa aagtccaac gaacggaac 
      ggatttaac aacgtcgag gattattta ctagtttca 
      ggtccagag agattatca aatgttact gaacacaat 
      gcgttacca caagcaaat tcagaagga ggcggcgcc 
      agtgaagct actgcagcg gtagatttg tcggaaatg 
      agcaccgag ctgtaactc atactctag cgatccaaa 
      taatttcat gtaatttta
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY