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Gene information
General information
TC001762 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 7611230..7614665
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001762
    iBeetle screen iB_03251
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature partially degenerated - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    larval musculature partially spotted - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature partially not present - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001762 RA
      MNRSEGLVQ RRNIIRDSG EGVKESQNN EKKQEHDED 
      IDEGDSKET RLTLMEEVL LLGLKDKEG YTSFWNDCI 
      SSGLRGCIL IELGLRGRV ELERAGMRR KALLTRKLI 
      LKNDTPTGD VLLDEALKH LKETDPPET VQSWIEYLS 
      GETWNPLKL RYQLKNVRE RLAKNLVEK GVLTTEKQN 
      FLLFDMTTH PLTDNVTKS RLIKKIQDA VLTKWVNDP 
      QRMDKRMLA LIFLAHASD VLENAFAPL NDDDYELAI 
      KRVRELLDL DFEQESVKP NTSEVLWAV FAAFTK
    CDS sequence
    • >TC001762 RA
      atgaatcgg agtgaaggg ctggtgcaa agacgaaac 
      ataatacgt gactcaggt gaaggtgtg aaagaatcg 
      cagaacaat gagaagaag caggagcac gacgaagac 
      atagacgag ggcgattcc aaagaaacc cgattaact 
      ttaatggaa gaagtatta ttattaggc ttaaaagac 
      aaagaaggt tacacatcg ttttggaac gactgcatt 
      tcgagcggt ctccggggg tgtattcta atcgagtta 
      ggtttacgt ggacgtgtg gaactcgag cgggctggt 
      atgcgccga aaggcgctc ctcacccgc aaacttatc 
      ctcaaaaac gacacacct accggagac gtcctccta 
      gatgaagct ttaaaacat ctcaaggaa acagatcca 
      ccagaaacg gttcaaagc tggattgag tacctcagc 
      ggcgaaact tggaacccc ctcaaactg cgctaccag 
      ctgaagaac gtgcgggaa cgcctagcg aaaaacctc 
      gtcgagaag ggggttctc acaacggag aagcaaaac 
      tttctgctg tttgatatg accacgcac cctctgacc 
      gataacgtc accaaaagc cgtttaata aaaaagatc 
      caggatgcc gtacttacg aagtgggtg aacgacccc 
      cagcgcatg gacaagcgg atgttggcg ttgattttc 
      ctggcccat gctagtgac gttctggag aacgctttt 
      gcgccccta aatgacgac gactacgaa ttggcaata 
      aagcgagta cgagagtta cttgacttg gactttgag 
      caagaatca gtcaaacca aacacttcg gaggtactg 
      tgggccgtt ttcgccgct ttcacgaaa tga
    mRNA sequence
    • >TC001762 RA
      gggtgctga ttacttggc attgttaga ttttctgta 
      caaagggat taaattgga attgataag agcgtgacg 
      taatcgtag gacgtaatc actttttcc cgatttttg 
      attttttat ccaaaaaaa aggcttgaa ggaaagtga 
      gcgaatttg tcgattttt cggacggag tggttttct 
      gccagcagc ctacatgct tttgacatt tgccatgtt 
      gatggaagt cagttaaac gtcaagtct tgtagaaaa 
      taaataagt tatttgata tttaattaa aatgaatcg 
      gagtgaagg gctggtgca aagacgaaa cataatacg 
      tgactcagg tgaaggtgt gaaagaatc gcagaacaa 
      tgagaagaa gcaggagca cgacgaaga catagacga 
      gggcgattc caaagaaac ccgattaac tttaatgga 
      agaagtatt attattagg cttaaaaga caaagaagg 
      ttacacatc gttttggaa cgactgcat ttcgagcgg 
      tctccgggg gtgtattct aatcgagtt aggtttacg 
      tggacgtgt ggaactcga gcgggctgg tatgcgccg 
      aaaggcgct cctcacccg caaacttat cctcaaaaa 
      cgacacacc taccggaga cgtcctcct agatgaagc 
      tttaaaaca tctcaagga aacagatcc accagaaac 
      ggttcaaag ctggattga gtacctcag cggcgaaac 
      ttggaaccc cctcaaact gcgctacca gctgaagaa 
      cgtgcggga acgcctagc gaaaaacct cgtcgagaa 
      gggggttct cacaacgga gaagcaaaa ctttctgct 
      gtttgatat gaccacgca ccctctgac cgataacgt 
      caccaaaag ccgtttaat aaaaaagat ccaggatgc 
      cgtacttac gaagtgggt gaacgaccc ccagcgcat 
      ggacaagcg gatgttggc gttgatttt cctggccca 
      tgctagtga cgttctgga gaacgcttt tgcgcccct 
      aaatgacga cgactacga attggcaat aaagcgagt 
      acgagagtt acttgactt ggactttga gcaagaatc 
      agtcaaacc aaacacttc ggaggtact gtgggccgt 
      tttcgccgc tttcacgaa atgaaaata aactgtttt 
      tgctactaa attttaact ataaataaa ttacgttga 
      aatattgaa gttatcagt tttgtattt aatgtgatg 
      ttagtttgt acattggaa attttttct atgtggagt 
      acacaggct agactagga taatcagtt tcagtggta 
      atacacagg gtgtttagc ttcgttgat gtttagagg 
      cttcggaca tcaggaaac tatgtactc caactttta 
      tttgtacat aacttcgaa ctaaatgta ttgttcatg 
      tatgtcaaa tattaccta attattatt attttataa 
      attataatt ctactctca ttgcaggag ttagcctta 
      atttgtatt aaaatattc ccattttct ttataaaaa 
      taaagttgt atcaatatt