Status
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Gene information
General information
TC031574
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001749
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7995640..8008547
Sequence information
Transcripts / Proteins (3) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031574
    iBeetle screen iB_03248

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Fertility
    egg chamber partially degenerated - Number of animals showing the phenotype: 2

    egg chamber late partiallynot present - Number of animals showing the phenotype: 2

    ovary without detectable defects - Number of animals showing the phenotype: 2
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031574 RA
      MEYMQYENI WLTKSIYED AETKYYENL AKASVTPLA 
      GEVAKARQL LIQSRDSFK DAPASETQS TDHGAIKFF 
      EKKAVEFEK TINAMVESI KQLELRLSK VEKSVNSAP 
      KPAPAPKPA ADDDDDVDL FGSESEEEN EAAAKLREE 
      RLAAYNAKK SKKPALIAK SNVILDVKP WDDETDMKE 
      LENQVRKIS SDGLLWGAA KLVPLAYGI HKLQISCVV 
      EDEKISIDW LQDEITAIE DYVQSVDIA AFNKI
    • >TC031574 RB
      MPPMSPQPV VISDESSSD EEDEMETLF IISSDSENE 
      KDDHEPDGA DGEKMELKD GENEDSENA AMALVEMSR 
      TTRQTDEAN KENIDRSNI SSEKDPGDL SEGAYGYEN 
      ADDIPLPEL LEIHGKQLV SGFEFGEAT KRRAQQNMQ 
      SARNKTQGR EEPSTSESA DDSKLTQVH KTPHSRKRQ 
      RVASARTKR QSERDFSKV SRQVPECLR GHPDCVEVW 
      DFMIYKEEA LLKLRNPRL FNDFKSVTP RMRAILLDW 
      LMEVAAVYH LRRVTYYLS VDYFDRFLS IRPDIPKSL 
      LQLVGITCL YIAAKVEEI YPPNLNEFS YVCDGACQS 
      KDMISCEVL ILNSLGWEV VLTTPTDWL NLYMQLHHK 
      STDIVRTKL NMDFNKDFV FPQYSAYQF TRASQLIDL 
      LSLDPGFLK FGYSVIAAA AMYYMYGRD IALAVSGFD 
      WSQLEPCVD YMEVFYIII KDAPDPRLY SCLGGPHPE 
      EMSRLSAGL LRRVQSGPQ SENYSFQTH IASMEYFET 
      ATIYRLNKL RPSTANEDT TSVPATEEN NETVDLTEE 
      LENLTELAK EDASATTVD HNLMTLDEI FELILGQEG 
      TAMEYMQYE NIWLTKSIY EDAETKYYE NLAKASVTP 
      LAGEVAKAR QLLIQSRDS FKDAPASET QSTDHGAIK 
      FFEKKAVEF EKTINAMVE SIKQLELRL SKVEKSVNS 
      APKPAPAPK PAADDDDDV DLFGSESEE ENEAAAKLR 
      EERLAAYNA KKSKKPALI AKSNVILDV KPWDDETDM 
      KELENQVRK ISSDGLLWG AAKLVPLAY GIHKLQISC 
      VVEDEKISI DWLQDEITA IEDYVQSVD IAAFNKI
    • >TC031574 RC
      MPPMSPQPV VISDESSSD EEDEMETLF IISSDSENE 
      KDDHEPDGA DGEKMELKD GENEDSENA AMALVEMSR 
      TTRQTDEAN KENIDRSNI SSEKDPGDL SEGAYGYEN 
      ADDIPLPEL LEIHGKQLV SGFEFGEAT KRRAQQNMQ 
      SARNKTQGR EEPSTSESA DDSKLTQVH KTPHSRKRQ 
      RVASARTKR QSERDFSKV SRQVPECLR GHPDCVEVW 
      DFMIYKEEA LLKLRNPRL FNDFKSVTP RMRAILLDW 
      LMEVAAVYH LRRVTYYLS VDYFDRFLS IRPDIPKSL 
      LQLVGITCL YIAAKVEEI YPPNLNEFS YVCDGACQS 
      KDMISCEVL ILNSLGWEV VLTTPTDWL NLYMQLHHK 
      STDIVRTKL NMDFNKDFV FPQYSAYQF TRASQLIDL 
      LSLDPGFLK FGYSVIAAA AMYYMYGRD IALAVSGFD 
      WSQLEPCVD YMEVFYIII KDAPDPRLY SCLGGPHPE 
      EMSRLSAGL LRRVQSGPQ SENYSFQTH IASMEYFET 
      ATIYRLNKL RPSTANEDT TSVPATEEN NETVDLTEE 
      LENLTELAK EDASATTVD HNLMTLDEI FELILGQEG 
      IKMCIL
    CDS sequence
    • >TC031574 RA
      atggagtac atgcaatac gagaatatt tggctcacc 
      aaatcaatt tacgaggac gcagagaca aagtattac 
      gagaacttg gcaaaggcg tccgtaact cctttggcg 
      ggcgaagtt gccaaggcg cgacaactt cttattcaa 
      agtagggat tcttttaaa gatgcccct gcgtccgag 
      acccaatcc actgatcac ggcgcgatc aaattcttc 
      gagaaaaag gccgtcgaa tttgagaaa actattaac 
      gcgatggtg gagagcata aaacagcta gaattgcgt 
      ctttccaaa gtcgagaaa agcgtcaat tccgctccc 
      aaacctgca ccagccccg aaacccgcc gctgacgac 
      gacgacgac gtcgatctg ttcggctcc gaatccgag 
      gaggagaac gaagccgcc gccaagctg agggaggag 
      cgtctggct gcttacaac gcgaaaaag tctaaaaag 
      ccggcttta atcgcaaaa tcgaacgtg atcctcgac 
      gtgaagccc tgggatgac gaaaccgac atgaaagag 
      ctcgaaaac caagtcagg aagatttca tcggacggc 
      ctcctctgg ggggccgcc aaactggtc cctttggct 
      tacggcatc cacaaattg caaatctcg tgtgttgtt 
      gaagacgag aaaatttca attgactgg ctacaggac 
      gaaatcaca gcaattgaa gactacgtc cagagtgtg 
      gatattgcg gccttcaac aagatctaa
    • >TC031574 RB
      atgcctcct atgtcgcca cagccagta gttattagc 
      gatgagtcg tcaagtgat gaagaagac gaaatggaa 
      acgcttttc atcatttca agcgactcg gaaaacgaa 
      aaagatgac catgagccg gatggagct gatggggaa 
      aaaatggaa ctaaaggat ggtgaaaat gaggacagc 
      gagaacgct gcgatggct ttggtcgaa atgagccgc 
      acgactcgt caaaccgac gaagcaaac aaagagaac 
      atagaccgc agcaacata tcaagcgaa aaagatccg 
      ggtgacttg tccgagggc gcgtacggg tacgaaaat 
      gccgacgat attccgcta cccgaactg ctcgaaatc 
      cacggtaaa caattagtc tccgggttc gagtttggc 
      gaggcaacg aaacgtcgc gcccaacaa aacatgcaa 
      tcggctcga aacaaaaca caagggagg gaagaaccg 
      tcaacgtcc gagtccgct gacgattcc aaattaaca 
      caagtgcac aaaacgcca cattcccgc aagcggcag 
      cgcgtggct tcggcgcga accaagcga cagtccgag 
      agagatttc agtaaagtt tcccgccaa gtccccgag 
      tgccttcgg ggtcacccc gattgcgtc gaggtctgg 
      gatttcatg atttacaaa gaggaggct ttgctcaaa 
      ttgcgaaac ccgagactg tttaacgac tttaaaagc 
      gttacgccg cgcatgcgg gcgatactt cttgattgg 
      ttgatggaa gttgccgcc gtttaccac ctcaggaga 
      gtcacgtat tatttaagt gttgattat ttcgaccgt 
      ttcctgtct ataagaccc gatatcccc aaaagtctg 
      ctacagcta gtggggatc acttgtttg tacattgct 
      gccaaagtc gaggagatt tatcctccg aatcttaac 
      gaattttcc tacgtctgt gatggcgca tgccaatcc 
      aaagacatg atctcgtgt gaagtttta attttaaat 
      agtttaggt tgggaggtt gttctcaca acgcctact 
      gactggctt aatttatac atgcagttg caccataag 
      agtactgat atcgtccga actaaactc aatatggac 
      ttcaataaa gacttcgtt tttccacaa tattccgcc 
      tatcagttc actcgtgct tcgcaactg attgatttg 
      ctgtcactt gatcccgga tttcttaaa tttggatac 
      agtgtgatt gctgcagcc gcaatgtac tacatgtac 
      ggacgcgac atcgcctta gctgtctca ggtttcgat 
      tggtcgcaa ctggagcct tgcgtcgac tacatggag 
      gtgttctac ataattatt aaagacgcc cccgatccg 
      cgtctgtac tcctgtctc ggtggccct catcccgag 
      gaaatgagc cgactttcg gccgggctt ctccgccgg 
      gtgcaatcg ggaccccaa agtgaaaat tactcgttt 
      caaacccac attgccagc atggagtac tttgagaca 
      gcaacaatc tacagattg aataaatta cggccttct 
      actgctaat gaagatacc acaagtgtt cctgcaaca 
      gaggaaaac aatgaaacg gttgatttg actgaagaa 
      cttgagaat ttaacggag ctggcaaaa gaggatgcc 
      agcgctact actgttgac cacaatttg atgactttg 
      gacgaaatt tttgagctt attttaggc caagaaggg 
      accgcaatg gagtacatg caatacgag aatatttgg 
      ctcaccaaa tcaatttac gaggacgca gagacaaag 
      tattacgag aacttggca aaggcgtcc gtaactcct 
      ttggcgggc gaagttgcc aaggcgcga caacttctt 
      attcaaagt agggattct tttaaagat gcccctgcg 
      tccgagacc caatccact gatcacggc gcgatcaaa 
      ttcttcgag aaaaaggcc gtcgaattt gagaaaact 
      attaacgcg atggtggag agcataaaa cagctagaa 
      ttgcgtctt tccaaagtc gagaaaagc gtcaattcc 
      gctcccaaa cctgcacca gccccgaaa cccgccgct 
      gacgacgac gacgacgtc gatctgttc ggctccgaa 
      tccgaggag gagaacgaa gccgccgcc aagctgagg 
      gaggagcgt ctggctgct tacaacgcg aaaaagtct 
      aaaaagccg gctttaatc gcaaaatcg aacgtgatc 
      ctcgacgtg aagccctgg gatgacgaa accgacatg 
      aaagagctc gaaaaccaa gtcaggaag atttcatcg 
      gacggcctc ctctggggg gccgccaaa ctggtccct 
      ttggcttac ggcatccac aaattgcaa atctcgtgt 
      gttgttgaa gacgagaaa atttcaatt gactggcta 
      caggacgaa atcacagca attgaagac tacgtccag 
      agtgtggat attgcggcc ttcaacaag atctaa
    • >TC031574 RC
      atgcctcct atgtcgcca cagccagta gttattagc 
      gatgagtcg tcaagtgat gaagaagac gaaatggaa 
      acgcttttc atcatttca agcgactcg gaaaacgaa 
      aaagatgac catgagccg gatggagct gatggggaa 
      aaaatggaa ctaaaggat ggtgaaaat gaggacagc 
      gagaacgct gcgatggct ttggtcgaa atgagccgc 
      acgactcgt caaaccgac gaagcaaac aaagagaac 
      atagaccgc agcaacata tcaagcgaa aaagatccg 
      ggtgacttg tccgagggc gcgtacggg tacgaaaat 
      gccgacgat attccgcta cccgaactg ctcgaaatc 
      cacggtaaa caattagtc tccgggttc gagtttggc 
      gaggcaacg aaacgtcgc gcccaacaa aacatgcaa 
      tcggctcga aacaaaaca caagggagg gaagaaccg 
      tcaacgtcc gagtccgct gacgattcc aaattaaca 
      caagtgcac aaaacgcca cattcccgc aagcggcag 
      cgcgtggct tcggcgcga accaagcga cagtccgag 
      agagatttc agtaaagtt tcccgccaa gtccccgag 
      tgccttcgg ggtcacccc gattgcgtc gaggtctgg 
      gatttcatg atttacaaa gaggaggct ttgctcaaa 
      ttgcgaaac ccgagactg tttaacgac tttaaaagc 
      gttacgccg cgcatgcgg gcgatactt cttgattgg 
      ttgatggaa gttgccgcc gtttaccac ctcaggaga 
      gtcacgtat tatttaagt gttgattat ttcgaccgt 
      ttcctgtct ataagaccc gatatcccc aaaagtctg 
      ctacagcta gtggggatc acttgtttg tacattgct 
      gccaaagtc gaggagatt tatcctccg aatcttaac 
      gaattttcc tacgtctgt gatggcgca tgccaatcc 
      aaagacatg atctcgtgt gaagtttta attttaaat 
      agtttaggt tgggaggtt gttctcaca acgcctact 
      gactggctt aatttatac atgcagttg caccataag 
      agtactgat atcgtccga actaaactc aatatggac 
      ttcaataaa gacttcgtt tttccacaa tattccgcc 
      tatcagttc actcgtgct tcgcaactg attgatttg 
      ctgtcactt gatcccgga tttcttaaa tttggatac 
      agtgtgatt gctgcagcc gcaatgtac tacatgtac 
      ggacgcgac atcgcctta gctgtctca ggtttcgat 
      tggtcgcaa ctggagcct tgcgtcgac tacatggag 
      gtgttctac ataattatt aaagacgcc cccgatccg 
      cgtctgtac tcctgtctc ggtggccct catcccgag 
      gaaatgagc cgactttcg gccgggctt ctccgccgg 
      gtgcaatcg ggaccccaa agtgaaaat tactcgttt 
      caaacccac attgccagc atggagtac tttgagaca 
      gcaacaatc tacagattg aataaatta cggccttct 
      actgctaat gaagatacc acaagtgtt cctgcaaca 
      gaggaaaac aatgaaacg gttgatttg actgaagaa 
      cttgagaat ttaacggag ctggcaaaa gaggatgcc 
      agcgctact actgttgac cacaatttg atgactttg 
      gacgaaatt tttgagctt attttaggc caagaaggg 
      attaagatg tgtatttta tga
    mRNA sequence
    • >TC031574 RA
      tggggattt tattttttg tgttggcga ccctgtcga 
      cataacctt ttcgagaac agctgtctc cccatataa 
      cagagtaca agaaatcta ttaaaaaac agcaaaaat 
      tctagccgc aatggagta catgcaata cgagaatat 
      ttggctcac caaatcaat ttacgagga cgcagagac 
      aaagtatta cgagaactt ggcaaaggc gtccgtaac 
      tcctttggc gggcgaagt tgccaaggc gcgacaact 
      tcttattca aagtaggga ttcttttaa agatgcccc 
      tgcgtccga gacccaatc cactgatca cggcgcgat 
      caaattctt cgagaaaaa ggccgtcga atttgagaa 
      aactattaa cgcgatggt ggagagcat aaaacagct 
      agaattgcg tctttccaa agtcgagaa aagcgtcaa 
      ttccgctcc caaacctgc accagcccc gaaacccgc 
      cgctgacga cgacgacga cgtcgatct gttcggctc 
      cgaatccga ggaggagaa cgaagccgc cgccaagct 
      gagggagga gcgtctggc tgcttacaa cgcgaaaaa 
      gtctaaaaa gccggcttt aatcgcaaa atcgaacgt 
      gatcctcga cgtgaagcc ctgggatga cgaaaccga 
      catgaaaga gctcgaaaa ccaagtcag gaagatttc 
      atcggacgg cctcctctg gggggccgc caaactggt 
      ccctttggc ttacggcat ccacaaatt gcaaatctc 
      gtgtgttgt tgaagacga gaaaatttc aattgactg 
      gctacagga cgaaatcac agcaattga agactacgt 
      ccagagtgt ggatattgc ggccttcaa caagatcta 
      agcgaccct ttgtacttt tttatttaa ataaataaa 
      gtggccccg gtttcattt ccaaataaa tatagttaa 
      ttttt
    • >TC031574 RB
      aagatatcc aaatttgtt ctttatcgc accaggtat 
      taaagacca aaagccatc ctcatggaa aatcgtaag 
      tacgcgcat gctttcatc tttgccgcg tagtatttg 
      ttgatattg ttcagaaat aatgtgatt ctcacctga 
      taaacccaa atgtagaag ttcgtgaaa cggtccctt 
      taaaccctt taactgtgt tttaaaaat ttagttttc 
      cgtgattta ttgtgcctc aaaacgagt ttttttgtt 
      tgattttga tctcttggt ggtaattta gctccgtgg 
      gtgatatta ttatcagct gttaaaacc gccaacatg 
      ttattacaa agaaccagc tgcccctgc tgtagaatc 
      ggcagaacc tcagccagg accaagtgg tgttgagcc 
      gattgacgt tgatattat tgagattgt cgatagccc 
      ggaacccgg gttcccagc tggtgaaga agctgcagc 
      cttgcaacc ctacggtgg gaacttcgc ggcggacac 
      atttgattc agacgttga tgattttct ggatgaggc 
      gttcgaact tttcattct gcaaccaca acgttggca 
      acagtttcg gcagttttg ctacaatcc ttactacaa 
      ttcgtcgtt gatgttcga catgcctcc tatgtcgcc 
      acagccagt agttattag cgatgagtc gtcaagtga 
      tgaagaaga cgaaatgga aacgctttt catcatttc 
      aagcgactc ggaaaacga aaaagatga ccatgagcc 
      ggatggagc tgatgggga aaaaatgga actaaagga 
      tggtgaaaa tgaggacag cgagaacgc tgcgatggc 
      tttggtcga aatgagccg cacgactcg tcaaaccga 
      cgaagcaaa caaagagaa catagaccg cagcaacat 
      atcaagcga aaaagatcc gggtgactt gtccgaggg 
      cgcgtacgg gtacgaaaa tgccgacga tattccgct 
      acccgaact gctcgaaat ccacggtaa acaattagt 
      ctccgggtt cgagtttgg cgaggcaac gaaacgtcg 
      cgcccaaca aaacatgca atcggctcg aaacaaaac 
      acaagggag ggaagaacc gtcaacgtc cgagtccgc 
      tgacgattc caaattaac acaagtgca caaaacgcc 
      acattcccg caagcggca gcgcgtggc ttcggcgcg 
      aaccaagcg acagtccga gagagattt cagtaaagt 
      ttcccgcca agtccccga gtgccttcg gggtcaccc 
      cgattgcgt cgaggtctg ggatttcat gatttacaa 
      agaggaggc tttgctcaa attgcgaaa cccgagact 
      gtttaacga ctttaaaag cgttacgcc gcgcatgcg 
      ggcgatact tcttgattg gttgatgga agttgccgc 
      cgtttacca cctcaggag agtcacgta ttatttaag 
      tgttgatta tttcgaccg tttcctgtc tataagacc 
      cgatatccc caaaagtct gctacagct agtggggat 
      cacttgttt gtacattgc tgccaaagt cgaggagat 
      ttatcctcc gaatcttaa cgaattttc ctacgtctg 
      tgatggcgc atgccaatc caaagacat gatctcgtg 
      tgaagtttt aattttaaa tagtttagg ttgggaggt 
      tgttctcac aacgcctac tgactggct taatttata 
      catgcagtt gcaccataa gagtactga tatcgtccg 
      aactaaact caatatgga cttcaataa agacttcgt 
      ttttccaca atattccgc ctatcagtt cactcgtgc 
      ttcgcaact gattgattt gctgtcact tgatcccgg 
      atttcttaa atttggata cagtgtgat tgctgcagc 
      cgcaatgta ctacatgta cggacgcga catcgcctt 
      agctgtctc aggtttcga ttggtcgca actggagcc 
      ttgcgtcga ctacatgga ggtgttcta cataattat 
      taaagacgc ccccgatcc gcgtctgta ctcctgtct 
      cggtggccc tcatcccga ggaaatgag ccgactttc 
      ggccgggct tctccgccg ggtgcaatc gggacccca 
      aagtgaaaa ttactcgtt tcaaaccca cattgccag 
      catggagta ctttgagac agcaacaat ctacagatt 
      gaataaatt acggccttc tactgctaa tgaagatac 
      cacaagtgt tcctgcaac agaggaaaa caatgaaac 
      ggttgattt gactgaaga acttgagaa tttaacgga 
      gctggcaaa agaggatgc cagcgctac tactgttga 
      ccacaattt gatgacttt ggacgaaat ttttgagct 
      tattttagg ccaagaagg gaccgcaat ggagtacat 
      gcaatacga gaatatttg gctcaccaa atcaattta 
      cgaggacgc agagacaaa gtattacga gaacttggc 
      aaaggcgtc cgtaactcc tttggcggg cgaagttgc 
      caaggcgcg acaacttct tattcaaag tagggattc 
      ttttaaaga tgcccctgc gtccgagac ccaatccac 
      tgatcacgg cgcgatcaa attcttcga gaaaaaggc 
      cgtcgaatt tgagaaaac tattaacgc gatggtgga 
      gagcataaa acagctaga attgcgtct ttccaaagt 
      cgagaaaag cgtcaattc cgctcccaa acctgcacc 
      agccccgaa acccgccgc tgacgacga cgacgacgt 
      cgatctgtt cggctccga atccgagga ggagaacga 
      agccgccgc caagctgag ggaggagcg tctggctgc 
      ttacaacgc gaaaaagtc taaaaagcc ggctttaat 
      cgcaaaatc gaacgtgat cctcgacgt gaagccctg 
      ggatgacga aaccgacat gaaagagct cgaaaacca 
      agtcaggaa gatttcatc ggacggcct cctctgggg 
      ggccgccaa actggtccc tttggctta cggcatcca 
      caaattgca aatctcgtg tgttgttga agacgagaa 
      aatttcaat tgactggct acaggacga aatcacagc 
      aattgaaga ctacgtcca gagtgtgga tattgcggc 
      cttcaacaa gatctaagc gaccctttg tactttttt 
      atttaaata aataaagtg gccccggtt tcatttcca 
      aataaatat agttaattt tt
    • >TC031574 RC
      aagatatcc aaatttgtt ctttatcgc accaggtat 
      taaagacca aaagccatc ctcatggaa aatcgtaag 
      tacgcgcat gctttcatc tttgccgcg tagtatttg 
      ttgatattg ttcagaaat aatgtgatt ctcacctga 
      taaacccaa atgtagaag ttcgtgaaa cggtccctt 
      taaaccctt taactgtgt tttaaaaat ttagttttc 
      cgtgattta ttgtgcctc aaaacgagt ttttttgtt 
      tgattttga tctcttggt ggtaattta gctccgtgg 
      gtgatatta ttatcagct gttaaaacc gccaacatg 
      ttattacaa agaaccagc tgcccctgc tgtagaatc 
      ggcagaacc tcagccagg accaagtgg tgttgagcc 
      gattgacgt tgatattat tgagattgt cgatagccc 
      ggaacccgg gttcccagc tggtgaaga agctgcagc 
      cttgcaacc ctacggtgg gaacttcgc ggcggacac 
      atttgattc agacgttga tgattttct ggatgaggc 
      gttcgaact tttcattct gcaaccaca acgttggca 
      acagtttcg gcagttttg ctacaatcc ttactacaa 
      ttcgtcgtt gatgttcga catgcctcc tatgtcgcc 
      acagccagt agttattag cgatgagtc gtcaagtga 
      tgaagaaga cgaaatgga aacgctttt catcatttc 
      aagcgactc ggaaaacga aaaagatga ccatgagcc 
      ggatggagc tgatgggga aaaaatgga actaaagga 
      tggtgaaaa tgaggacag cgagaacgc tgcgatggc 
      tttggtcga aatgagccg cacgactcg tcaaaccga 
      cgaagcaaa caaagagaa catagaccg cagcaacat 
      atcaagcga aaaagatcc gggtgactt gtccgaggg 
      cgcgtacgg gtacgaaaa tgccgacga tattccgct 
      acccgaact gctcgaaat ccacggtaa acaattagt 
      ctccgggtt cgagtttgg cgaggcaac gaaacgtcg 
      cgcccaaca aaacatgca atcggctcg aaacaaaac 
      acaagggag ggaagaacc gtcaacgtc cgagtccgc 
      tgacgattc caaattaac acaagtgca caaaacgcc 
      acattcccg caagcggca gcgcgtggc ttcggcgcg 
      aaccaagcg acagtccga gagagattt cagtaaagt 
      ttcccgcca agtccccga gtgccttcg gggtcaccc 
      cgattgcgt cgaggtctg ggatttcat gatttacaa 
      agaggaggc tttgctcaa attgcgaaa cccgagact 
      gtttaacga ctttaaaag cgttacgcc gcgcatgcg 
      ggcgatact tcttgattg gttgatgga agttgccgc 
      cgtttacca cctcaggag agtcacgta ttatttaag 
      tgttgatta tttcgaccg tttcctgtc tataagacc 
      cgatatccc caaaagtct gctacagct agtggggat 
      cacttgttt gtacattgc tgccaaagt cgaggagat 
      ttatcctcc gaatcttaa cgaattttc ctacgtctg 
      tgatggcgc atgccaatc caaagacat gatctcgtg 
      tgaagtttt aattttaaa tagtttagg ttgggaggt 
      tgttctcac aacgcctac tgactggct taatttata 
      catgcagtt gcaccataa gagtactga tatcgtccg 
      aactaaact caatatgga cttcaataa agacttcgt 
      ttttccaca atattccgc ctatcagtt cactcgtgc 
      ttcgcaact gattgattt gctgtcact tgatcccgg 
      atttcttaa atttggata cagtgtgat tgctgcagc 
      cgcaatgta ctacatgta cggacgcga catcgcctt 
      agctgtctc aggtttcga ttggtcgca actggagcc 
      ttgcgtcga ctacatgga ggtgttcta cataattat 
      taaagacgc ccccgatcc gcgtctgta ctcctgtct 
      cggtggccc tcatcccga ggaaatgag ccgactttc 
      ggccgggct tctccgccg ggtgcaatc gggacccca 
      aagtgaaaa ttactcgtt tcaaaccca cattgccag 
      catggagta ctttgagac agcaacaat ctacagatt 
      gaataaatt acggccttc tactgctaa tgaagatac 
      cacaagtgt tcctgcaac agaggaaaa caatgaaac 
      ggttgattt gactgaaga acttgagaa tttaacgga 
      gctggcaaa agaggatgc cagcgctac tactgttga 
      ccacaattt gatgacttt ggacgaaat ttttgagct 
      tattttagg ccaagaagg gattaagat gtgtatttt 
      atgacttgt tcttaagtg ttagtttcc tcgttcgta 
      ttatttatc tcactacac tagtgcctt ttctaaact 
      gtgatcaat agttttaaa tattgagat ctatttgta 
      aagaagcgt tgtaacctt tgtaaattt ttgctaaac 
      atttcactt caattttag tcatagaca aatcatatt 
      tattagcat aaattagtc aattgtaat atattcgat 
      tttaactta attgtttta gcgtgacgc gagatctat 
      gatgtaact tttatagcg tttggtgtc gttccagtt 
      ttagtcaag caaattgtt ttggattat tttaatcgt 
      aatttagag tcttgtagt cacttaaaa agactgtat 
      tttatacat atttttgta tatttggaa tttgaaata 
      aactgtttt ggtactact tgagtttgg ggaatggct 
      gcttgccca aataaaaat tttgcaaaa aa
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