Status
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Gene information
General information
TC031573
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001748,TC001747
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8028505..8052253
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC031573
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03247

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC031573 RA
      MLEPRDVAE KKLNAETNR LIGEVQLLQ YGNFLDKYS 
      ADLNHFKEK LPNRFNVRL EPILLQSSR TENVSLLHL 
      VHSDNKLLS RILASLAAV CEEINLLVE EARTYYPVF 
      VFYGEGKNS TKEDLKSIS SVVEVLQKV SCFIERCYH 
      VIALLFQQL CAILGKEYN TIRSGTCFP ELLDCLAEL 
      LVCLLTFDS LFSNQTLLK DHWLLYWRS VKGILHNPG 
      KFQLTREQV RSLDKNLAS IDNTLLTGN IFKTALEKC 
      LDEKVILVM KNSSLSNEI SAYINQLLT ELERDEENI 
      FFTQVWLQV NCLVVLEHC LFGSTDKKL VKRLIEVNK 
      KASACTLCG NVLWYPEQF LLKHVPSLS KFLDEKTFD 
      NIRLAKIAL RSQNLPKET HLLSLQACH WMIEIDRIT 
      QRNLTSFDV KHLHETSNL LIDGIKLIK KISEVVKWI 
      TNLHADKDR PMAKSVLLS LCRLVEILK CFQFIFHKK 
      LQPLVYIIS LILQHLSHK ALTLLRNIR KSLTQEKSY 
      KEQQLDVLS ALNVCEQNL KGPNTNQRI LIAKLSLSA 
      SGLSCDHLT EIRSVINRL ELICCVHSI LNRLCDCSF 
      LYWHQIYML PVYFSKLIE TKADLSRHY LILSALSDC 
      ALIAGDKIS GSTDTCLKQ NLFGPLNQI VETNLRLQT 
      HLHLQLPPS DPFKNTLPL NFAKFNPAR MDKYYISIK 
      KETEHYLST MFYNLTTVV LHDWKTYGE MRRLARLQY 
      GLETVEDNL PMQTLEQGL DVLEIMRNI NVFVGKYLY 
      NLNNQVFVE ESSNNKHLN TINISHVAN SIRTHGIGI 
      MNTTVNFTY QFLQKKFYI FSQFMFDEH IKSRLLKDM 
      RFFSEHKLD LSQMYPYER ADKFNIGIR NLGLNQNGL 
      SYLDLFRKL ITQIGNAMG YVRLIRSGG RRFLADSTC 
      FIPNLKEID QLNELISQE AASELTKQA ANCLMSDLK 
      ALVENFEEA TEYFKLLVK VFIPVFRNP SNIHLKNFY 
      IIVPPLTIN FVEHSLTCK ERLNKKNKN DAAFTDDGF 
      VIGLAYIIE LLGQESQLN SLHWFNSVQ AKFELDKKK 
      IEEQKAVAS KQDDKLQQT LQLSEKRIN AFQRRDMHP 
      WRSPFFLIV LCVFVHVLV LKAAFDIYL SSPIEHGTP 
      FKSTEKPPA GRAVIIVSD GLRAKDLFG EKQEMALPN 
      LTKLRKTRA SWGVARTEL PTVLLAGAY EHSASIFLG 
      LSGNRNSFD SVINQSTFA WCWGGPQIA EMCSEDDLG 
      HIHSYVYDS ANPDFGLRV YEDVETFIL ERSNCEVYC 
      LDFIERGNV FFLHLSGRG FKPNSMEYY NNIGLVDKI 
      IPKIEELFE TAFPDDSTA YIFTSGSGV TDWVSEPAN 
      EIPFIAWGA GVKTINGQK DIKQVDIAL LISALIGLN 
      YPVNSIGTL PRDLIDTSK ENLGEMSLS NVRQLLETY 
      LLKSQWKMN NAYFHGFDK IILGKFRQL EDLLSDKKF 
      AQFNQECDL FIGYLTQGP EYQVSILIS VTIGFAAFI 
      IYVAVFNIP VRRQSRVTT STIKFIQIF IIIPAYLNV 
      LIVLKAQKS SLLYYLYFL FPIGMIHAL VRRYPQIVA 
      AMKYIRRYG FKNTAKRSV LFLVGLRLL AESFYNRMA 
      LAVLMFVMG LAVYVTPSM RRHTVSAQK LAWLAATVF 
      LGVLQLIPV TGIVTKFSA YMMGYGFWW ITMDMVIVR 
      QYEHPFYNI KLIEIVDLQ HFFVKLTPL YTIAVQFEF 
      IAHDSWWNV FAWIWALYP VVIIPCSSQ HVFVRLTSI 
      FVAFAPFCL MVTFGFEMF FLIIFVLGL YAWLTIENT 
      CYGYEKIMR ANFLEEFEL NRRLTADDF RRGFFFVSF 
      VFVGFIGTG SSFDPEWLR TTFISAELF TMANFLFLR 
      ILIPFIYTS CTYRAINIF HKASTLTMF CAVLVFLDL 
      MLLELFFNL NNDGTWHEI STRLSCFIV MQLFVLALI 
      MLHGVGYFL TTLFYRLRL NCLLCHF
    CDS sequence
    • >TC031573 RA
      atgcttgaa ccccgagat gtagctgag aagaaatta 
      aatgctgaa accaataga ttaattggt gaagtacag 
      ctgcttcag tacggcaat tttttagat aagtacagt 
      gctgattta aatcatttt aaagagaag cttccaaat 
      agattcaac gtgcgatta gagccaatt ttacttcag 
      agctctaga actgaaaat gtgtctctg ttgcattta 
      gtacactca gataataag cttttgagt cgaattttg 
      gccagttta gctgctgtt tgtgaagaa attaattta 
      ctagtggag gaagccagg acgtactat cccgttttt 
      gttttctat ggggaaggg aaaaatagt acaaaggaa 
      gacctgaaa tcaatttcg tcagttgtt gaagttctg 
      caaaaagtt agttgtttt attgaacgt tgttaccac 
      gttattgct ttattattc caacaacta tgtgccatt 
      ctgggaaag gaatataat acgattcga tcaggaaca 
      tgctttcct gagttgtta gattgcttg gccgagttg 
      ttggtttgt ttgttgact tttgacagc ttattttca 
      aatcaaacg ttactaaag gatcattgg ttgttatac 
      tggaggtct gttaagggt attttacac aatccaggg 
      aagtttcag ctcactcga gaacaagtt aggtcgctt 
      gataaaaat ttggcaagc attgataat acacttttg 
      acgggaaat atatttaag actgcgtta gaaaaatgt 
      cttgatgaa aaagtgatc ctcgttatg aaaaacagc 
      agtctttcg aatgagatt tcggcctac attaatcag 
      ttactaacc gaactagaa cgggacgaa gaaaatata 
      ttttttacc caagtttgg ttgcaagtg aattgtctt 
      gttgttctg gaacattgc ttgtttggt tcgactgat 
      aaaaaacta gttaagaga ctgattgaa gtcaataaa 
      aaggcctct gcttgcacc ctctgtggg aatgttttg 
      tggtaccct gaacaattt ttgttaaaa catgttccg 
      tctttaagc aaatttctt gatgaaaaa acgtttgat 
      aatatcaga cttgcaaaa attgccttg cgaagtcag 
      aatttaccg aaggaaacg catctcctg tcgctccag 
      gcttgccat tggatgatt gagatagat agaatcacc 
      caaagaaat ttaaccagt tttgatgtc aaacatcta 
      cacgaaacg tccaattta ttaatagac gggataaag 
      ctaattaaa aaaatcagc gaagtggtc aaatggatt 
      acaaattta catgccgac aaagaccgg ccgatggcc 
      aaatccgtg cttttgtcg ctctgtcgc ctcgttgaa 
      attttgaag tgtttccaa tttattttc cataagaaa 
      ttgcaaccg cttgtttat ataatttcg ctcattttg 
      caacatttg tcgcacaaa gccttgaca ttgttgcgc 
      aatatacgt aagagttta actcaagaa aaatcgtac 
      aaggaacaa cagttagat gttctatcc gctttaaat 
      gtttgcgag caaaatcta aagggacct aacacaaac 
      caacgaatt ttaatagca aaattgtct ttgtcagcc 
      agtggatta tcctgcgat catttaact gagataagg 
      tctgtgata aaccgttta gagttaatt tgttgtgta 
      cattctata ttaaatcga ctatgtgat tgctcgttt 
      ttatattgg caccaaatc tatatgttg cctgtgtat 
      ttttcaaaa ctaattgaa accaaagct gacttatct 
      agacattat ttaatactc agtgcttta agtgattgt 
      gctcttatt gccggagat aaaatatcc ggatcgact 
      gacacttgt cttaaacaa aacctgttt ggaccttta 
      aatcaaatt gttgaaact aatttacgc cttcaaacg 
      catctacat ttacaactg cccccctca gatccattc 
      aaaaacacc ctaccatta aacttcgcc aaatttaat 
      ccagcgagg atggacaag tattacata agtattaaa 
      aaagaaact gaacattat ttgagtacg atgttttat 
      aatttaaca acggtggtc ttgcatgat tggaaaacc 
      tacggcgag atgagaaga ctagcacgt cttcaatac 
      gggttggaa acagtcgag gacaatctt ccaatgcaa 
      acacttgaa caaggttta gatgtttta gaaataatg 
      aggaatata aatgttttt gtcggaaaa tatttgtac 
      aacttaaac aatcaagta ttcgttgag gaatcaagc 
      aataataaa catttaaac accattaac atttcgcat 
      gttgccaac tccatccga actcacgga ataggtata 
      atgaacacg acggtcaat tttacctac cagtttttg 
      caaaaaaag ttctatatt ttttcgcaa tttatgttt 
      gatgagcat ataaagtcc cgcttgctg aaagacatg 
      aggtttttt tcggaacat aagctagat ctgagccag 
      atgtatccg tatgagcgg gcagataag ttcaatatc 
      gggatcaga aatttagga ttgaaccag aacggttta 
      agttacttg gacttgttc cggaaactg atcacacaa 
      attggcaat gccatgggt tacgtccgg ttaatccgc 
      tcaggtgga cgtcgtttt cttgccgac agcacttgt 
      tttatccca aatttaaaa gaaatcgac caattaaac 
      gagcttata agccaggag gccgcgtcc gaattgacg 
      aaacaagcc gcaaactgt ttgatgagc gatctaaag 
      gcactagtt gaaaatttt gaagaagca acagagtat 
      tttaaactt cttgttaag gtttttata ccggtcttc 
      agaaacccg agcaacata catttgaaa aatttttac 
      ataatcgtg ccaccactg acaataaat ttcgttgag 
      cattcgttg acttgcaaa gaacgatta aataagaaa 
      aataaaaat gacgccgca tttactgac gatgggttt 
      gttatcggg cttgcttac attattgaa cttttgggg 
      caagaaagc cagttaaat tcgctacac tggttcaac 
      tcagttcaa gccaagttt gaacttgat aaaaagaag 
      atcgaggaa cagaaagcc gttgcgagc aagcaagac 
      gataaattg caacagacc ttgcaactt tctgagaaa 
      cgaattaat gccttccaa cggcgagac atgcatccg 
      tggcgctcg ccgttcttt cttatcgtg ttgtgtgtc 
      ttcgtacac gtattagta ctgaaagcc gcatttgac 
      atctatctt tcgagcccc atcgaacat gggactcca 
      tttaaatca actgaaaaa ccgccggca ggccgcgcc 
      gttatcatc gtttctgat ggtcttcga gctaaagac 
      cttttcggg gaaaagcaa gaaatggca ttaccgaat 
      ttgactaaa ttacgtaaa actcgcgcc tcgtggggc 
      gtcgcccgc acggaactt ccgaccgtt ttgttagca 
      ggggcttat gagcactcg gcttcgatt tttctgggg 
      ctgtctgga aatcgaaac agttttgat tcggttatt 
      aaccagagt acgttcgcc tggtgctgg ggtggtccc 
      caaattgct gaaatgtgc agcgaagat gacctaggc 
      catattcat tcctacgtt tatgactct gcgaatccc 
      gatttcggc cttcgggtg tacgaagac gtcgagact 
      tttattctg gaaagatca aactgtgag gtttattgt 
      ctggacttt atagaacgg ggaaacgta tttttcttg 
      cacttgtct ggtcgtgga tttaaaccg aactcaatg 
      gaatattat aacaacatc gggctcgtt gacaaaatc 
      attccaaag atcgaggag ctgtttgag actgcattt 
      cccgacgat tccaccgcg tacattttt acttccggc 
      agtggggtg acagattgg gtgtcggaa cccgcaaat 
      gaaatcccc ttcatagcg tggggcgct ggcgttaaa 
      accataaac ggacagaaa gacatcaaa caggtggat 
      attgcgctt ttgatttca gccttaatt ggtttaaat 
      taccccgtg aattcaatc gggacttta ccgcgcgat 
      ttgattgat acatccaaa gaaaatttg ggcgaaatg 
      tcgctttca aatgttcgg caactcctg gaaacatat 
      ttgttgaaa agtcaatgg aagatgaac aatgcgtat 
      tttcacggt tttgacaaa ataattttg ggtaaattt 
      aggcaattg gaggatttg ctaagtgac aagaagttt 
      gcacaattt aaccaagag tgcgacctg tttatcgga 
      tacctaacc caaggtcct gaataccag gtctcgatt 
      ctaatttct gtaacaata ggatttgcg gcttttatt 
      atttatgta gccgttttc aatatacct gtacgcaga 
      caatcacgg gtcactacg tctacaata aaattcata 
      caaatattt attatcatc ccggcgtat ctgaacgtc 
      ttaattgtc ttaaaggct caaaaatct tcattgctg 
      tattaccta tatttccta tttcctatt ggtatgata 
      cacgcactt gtacgccgg taccctcaa atcgtggct 
      gccatgaaa tacatcagg cgttacggc tttaaaaac 
      actgccaaa aggtctgtt ttgtttttg gttggactt 
      cgtctatta gcggaatcg ttctacaat cgtatggct 
      ttggctgtc ttgatgttc gttatgggt ttggccgta 
      tatgtcaca ccgtcaatg agaagacat acagtgagt 
      gcgcaaaaa ttggcttgg cttgcagca acggttttt 
      ttgggtgtc ttgcagcta atccctgtt actgggata 
      gtaactaaa ttttcggct tacatgatg gggtatggt 
      ttctggtgg attactatg gatatggtg atagtacgt 
      cagtatgaa catccgttt tataacata aaattgata 
      gaaatagtt gatttgcaa cactttttt gtgaaatta 
      accccactg tatactatt gcggtccag tttgaattc 
      attgcacac gattcatgg tggaatgtt tttgcatgg 
      atttgggcc ctttatccc gttgttatt ataccttgc 
      tcgtcacaa catgttttc gtacgcctc acaagtata 
      tttgttgct tttgcacca ttttgctta atggtgaca 
      tttggtttt gaaatgttc tttttgata atatttgtg 
      ctggggctg tacgcttgg cttacgatt gagaatacg 
      tgctacggt tacgaaaaa attatgcgg gcgaatttt 
      ttggaagaa tttgagctc aaccgtaga ctgactgct 
      gacgacttc cgtcgaggg ttctttttt gtatctttc 
      gtttttgtc gggtttatt ggtaccgga agctccttt 
      gatccagag tggctgcgt accactttc atttctgct 
      gaactgttt acaatggca aatttccta tttttaagg 
      attctgata ccatttatc tacactagt tgtacttac 
      cgggctata aatattttt cataaagca agcaccctt 
      acgatgttt tgcgctgtt ttggtattt ttggattta 
      atgcttttg gaactcttt tttaatctt aataatgac 
      ggaacttgg catgaaatt tctaccagg ctttcatgc 
      ttcatcgtt atgcagctg tttgttttg gcactcata 
      atgttgcac ggtgtgggg tactttttg acgactcta 
      ttctataga cttagactg aattgcctt ttgtgtcat 
      ttttag
    mRNA sequence
    • >TC031573 RA
      tttcaaaag tggttccaa cctctgttc ctgttgtcg 
      tcaacctgt caaaatgtg ctttaagtt ggtagttgt 
      gttattaaa attgttttc tttttaaac gttttaaaa 
      caatgttat aattactgt aatagaaac tgtgtacgc 
      ttaaaacgt caataaaat gcttgaacc ccgagatgt 
      agctgagaa gaaattaaa tgctgaaac caatagatt 
      aattggtga agtacagct gcttcagta cggcaattt 
      tttagataa gtacagtgc tgatttaaa tcattttaa 
      agagaagct tccaaatag attcaacgt gcgattaga 
      gccaatttt acttcagag ctctagaac tgaaaatgt 
      gtctctgtt gcatttagt acactcaga taataagct 
      tttgagtcg aattttggc cagtttagc tgctgtttg 
      tgaagaaat taatttact agtggagga agccaggac 
      gtactatcc cgtttttgt tttctatgg ggaagggaa 
      aaatagtac aaaggaaga cctgaaatc aatttcgtc 
      agttgttga agttctgca aaaagttag ttgttttat 
      tgaacgttg ttaccacgt tattgcttt attattcca 
      acaactatg tgccattct gggaaagga atataatac 
      gattcgatc aggaacatg ctttcctga gttgttaga 
      ttgcttggc cgagttgtt ggtttgttt gttgacttt 
      tgacagctt attttcaaa tcaaacgtt actaaagga 
      tcattggtt gttatactg gaggtctgt taagggtat 
      tttacacaa tccagggaa gtttcagct cactcgaga 
      acaagttag gtcgcttga taaaaattt ggcaagcat 
      tgataatac acttttgac gggaaatat atttaagac 
      tgcgttaga aaaatgtct tgatgaaaa agtgatcct 
      cgttatgaa aaacagcag tctttcgaa tgagatttc 
      ggcctacat taatcagtt actaaccga actagaacg 
      ggacgaaga aaatatatt ttttaccca agtttggtt 
      gcaagtgaa ttgtcttgt tgttctgga acattgctt 
      gtttggttc gactgataa aaaactagt taagagact 
      gattgaagt caataaaaa ggcctctgc ttgcaccct 
      ctgtgggaa tgttttgtg gtaccctga acaattttt 
      gttaaaaca tgttccgtc tttaagcaa atttcttga 
      tgaaaaaac gtttgataa tatcagact tgcaaaaat 
      tgccttgcg aagtcagaa tttaccgaa ggaaacgca 
      tctcctgtc gctccaggc ttgccattg gatgattga 
      gatagatag aatcaccca aagaaattt aaccagttt 
      tgatgtcaa acatctaca cgaaacgtc caatttatt 
      aatagacgg gataaagct aattaaaaa aatcagcga 
      agtggtcaa atggattac aaatttaca tgccgacaa 
      agaccggcc gatggccaa atccgtgct tttgtcgct 
      ctgtcgcct cgttgaaat tttgaagtg tttccaatt 
      tattttcca taagaaatt gcaaccgct tgtttatat 
      aatttcgct cattttgca acatttgtc gcacaaagc 
      cttgacatt gttgcgcaa tatacgtaa gagtttaac 
      tcaagaaaa atcgtacaa ggaacaaca gttagatgt 
      tctatccgc tttaaatgt ttgcgagca aaatctaaa 
      gggacctaa cacaaacca acgaatttt aatagcaaa 
      attgtcttt gtcagccag tggattatc ctgcgatca 
      tttaactga gataaggtc tgtgataaa ccgtttaga 
      gttaatttg ttgtgtaca ttctatatt aaatcgact 
      atgtgattg ctcgttttt atattggca ccaaatcta 
      tatgttgcc tgtgtattt ttcaaaact aattgaaac 
      caaagctga cttatctag acattattt aatactcag 
      tgctttaag tgattgtgc tcttattgc cggagataa 
      aatatccgg atcgactga cacttgtct taaacaaaa 
      cctgtttgg acctttaaa tcaaattgt tgaaactaa 
      tttacgcct tcaaacgca tctacattt acaactgcc 
      cccctcaga tccattcaa aaacaccct accattaaa 
      cttcgccaa atttaatcc agcgaggat ggacaagta 
      ttacataag tattaaaaa agaaactga acattattt 
      gagtacgat gttttataa tttaacaac ggtggtctt 
      gcatgattg gaaaaccta cggcgagat gagaagact 
      agcacgtct tcaatacgg gttggaaac agtcgagga 
      caatcttcc aatgcaaac acttgaaca aggtttaga 
      tgttttaga aataatgag gaatataaa tgtttttgt 
      cggaaaata tttgtacaa cttaaacaa tcaagtatt 
      cgttgagga atcaagcaa taataaaca tttaaacac 
      cattaacat ttcgcatgt tgccaactc catccgaac 
      tcacggaat aggtataat gaacacgac ggtcaattt 
      tacctacca gtttttgca aaaaaagtt ctatatttt 
      ttcgcaatt tatgtttga tgagcatat aaagtcccg 
      cttgctgaa agacatgag gtttttttc ggaacataa 
      gctagatct gagccagat gtatccgta tgagcgggc 
      agataagtt caatatcgg gatcagaaa tttaggatt 
      gaaccagaa cggtttaag ttacttgga cttgttccg 
      gaaactgat cacacaaat tggcaatgc catgggtta 
      cgtccggtt aatccgctc aggtggacg tcgttttct 
      tgccgacag cacttgttt tatcccaaa tttaaaaga 
      aatcgacca attaaacga gcttataag ccaggaggc 
      cgcgtccga attgacgaa acaagccgc aaactgttt 
      gatgagcga tctaaaggc actagttga aaattttga 
      agaagcaac agagtattt taaacttct tgttaaggt 
      ttttatacc ggtcttcag aaacccgag caacataca 
      tttgaaaaa tttttacat aatcgtgcc accactgac 
      aataaattt cgttgagca ttcgttgac ttgcaaaga 
      acgattaaa taagaaaaa taaaaatga cgccgcatt 
      tactgacga tgggtttgt tatcgggct tgcttacat 
      tattgaact tttggggca agaaagcca gttaaattc 
      gctacactg gttcaactc agttcaagc caagtttga 
      acttgataa aaagaagat cgaggaaca gaaagccgt 
      tgcgagcaa gcaagacga taaattgca acagacctt 
      gcaactttc tgagaaacg aattaatgc cttccaacg 
      gcgagacat gcatccgtg gcgctcgcc gttctttct 
      tatcgtgtt gtgtgtctt cgtacacgt attagtact 
      gaaagccgc atttgacat ctatctttc gagccccat 
      cgaacatgg gactccatt taaatcaac tgaaaaacc 
      gccggcagg ccgcgccgt tatcatcgt ttctgatgg 
      tcttcgagc taaagacct tttcgggga aaagcaaga 
      aatggcatt accgaattt gactaaatt acgtaaaac 
      tcgcgcctc gtggggcgt cgcccgcac ggaacttcc 
      gaccgtttt gttagcagg ggcttatga gcactcggc 
      ttcgatttt tctggggct gtctggaaa tcgaaacag 
      ttttgattc ggttattaa ccagagtac gttcgcctg 
      gtgctgggg tggtcccca aattgctga aatgtgcag 
      cgaagatga cctaggcca tattcattc ctacgttta 
      tgactctgc gaatcccga tttcggcct tcgggtgta 
      cgaagacgt cgagacttt tattctgga aagatcaaa 
      ctgtgaggt ttattgtct ggactttat agaacgggg 
      aaacgtatt tttcttgca cttgtctgg tcgtggatt 
      taaaccgaa ctcaatgga atattataa caacatcgg 
      gctcgttga caaaatcat tccaaagat cgaggagct 
      gtttgagac tgcatttcc cgacgattc caccgcgta 
      catttttac ttccggcag tggggtgac agattgggt 
      gtcggaacc cgcaaatga aatcccctt catagcgtg 
      gggcgctgg cgttaaaac cataaacgg acagaaaga 
      catcaaaca ggtggatat tgcgctttt gatttcagc 
      cttaattgg tttaaatta ccccgtgaa ttcaatcgg 
      gactttacc gcgcgattt gattgatac atccaaaga 
      aaatttggg cgaaatgtc gctttcaaa tgttcggca 
      actcctgga aacatattt gttgaaaag tcaatggaa 
      gatgaacaa tgcgtattt tcacggttt tgacaaaat 
      aattttggg taaatttag gcaattgga ggatttgct 
      aagtgacaa gaagtttgc acaatttaa ccaagagtg 
      cgacctgtt tatcggata cctaaccca aggtcctga 
      ataccaggt ctcgattct aatttctgt aacaatagg 
      atttgcggc ttttattat ttatgtagc cgttttcaa 
      tatacctgt acgcagaca atcacgggt cactacgtc 
      tacaataaa attcataca aatatttat tatcatccc 
      ggcgtatct gaacgtctt aattgtctt aaaggctca 
      aaaatcttc attgctgta ttacctata tttcctatt 
      tcctattgg tatgataca cgcacttgt acgccggta 
      ccctcaaat cgtggctgc catgaaata catcaggcg 
      ttacggctt taaaaacac tgccaaaag gtctgtttt 
      gtttttggt tggacttcg tctattagc ggaatcgtt 
      ctacaatcg tatggcttt ggctgtctt gatgttcgt 
      tatgggttt ggccgtata tgtcacacc gtcaatgag 
      aagacatac agtgagtgc gcaaaaatt ggcttggct 
      tgcagcaac ggttttttt gggtgtctt gcagctaat 
      ccctgttac tgggatagt aactaaatt ttcggctta 
      catgatggg gtatggttt ctggtggat tactatgga 
      tatggtgat agtacgtca gtatgaaca tccgtttta 
      taacataaa attgataga aatagttga tttgcaaca 
      cttttttgt gaaattaac cccactgta tactattgc 
      ggtccagtt tgaattcat tgcacacga ttcatggtg 
      gaatgtttt tgcatggat ttgggccct ttatcccgt 
      tgttattat accttgctc gtcacaaca tgttttcgt 
      acgcctcac aagtatatt tgttgcttt tgcaccatt 
      ttgcttaat ggtgacatt tggttttga aatgttctt 
      tttgataat atttgtgct ggggctgta cgcttggct 
      tacgattga gaatacgtg ctacggtta cgaaaaaat 
      tatgcgggc gaatttttt ggaagaatt tgagctcaa 
      ccgtagact gactgctga cgacttccg tcgagggtt 
      cttttttgt atctttcgt ttttgtcgg gtttattgg 
      taccggaag ctcctttga tccagagtg gctgcgtac 
      cactttcat ttctgctga actgtttac aatggcaaa 
      tttcctatt tttaaggat tctgatacc atttatcta 
      cactagttg tacttaccg ggctataaa tatttttca 
      taaagcaag cacccttac gatgttttg cgctgtttt 
      ggtattttt ggatttaat gcttttgga actcttttt 
      taatcttaa taatgacgg aacttggca tgaaatttc 
      taccaggct ttcatgctt catcgttat gcagctgtt 
      tgttttggc actcataat gttgcacgg tgtggggta 
      ctttttgac gactctatt ctatagact tagactgaa 
      ttgcctttt gtgtcattt ttagtgaaa ttgaccgtt 
      tttgcagtt tgtgttgtc agtatttgt cttcttaaa 
      ttagactaa agtttgatg ttattt
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