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Gene information
General information
TC001746 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8052690..8057760
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001746
    iBeetle screen iB_03246

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001746 RA
      MSYPPPGGQ HPGPYPTPY NGQYPPMPP GQGYGTPPP 
      PGYGPPPGY GPPPQGYPP GQYPPPGQY PPPGHYGPP 
      PVINQPMGP GPGGAPGGW MNMPQGQVN CPPGLEYLT 
      MIDQLLVHQ KVELLEALT GFETKNKFT VKNSLGQKV 
      YYAVEDSDC LTRNCCGPI RPFDMNILD NYKNEVIHL 
      HRPLACDSC CFPCCLQSI EVSAPPGTV VGSVEQEWS 
      IFCPSFAIK NPSGETVLR IEGPFCTFS ICGDVEFKI 
      MSADGNTQV GKISKQWSG LIREMFTDT DYFGITFPM 
      DLDVRMKAV MLGACFLID AMFFEKSRN KEEDSPGML
    CDS sequence
    • >TC001746 RA
      atgtcgtac cctccacct ggtggacag caccctggg 
      ccttaccct acaccctac aatggtcag tatccaccg 
      atgcccccg ggtcagggc tatggaacg ccgccgccg 
      cccggctat ggcccccct ccggggtac ggaccgccg 
      cctcagggg tacccccca gggcagtac ccaccccca 
      gggcaatac ccgccccca ggacactat gggcctcca 
      ccagtgatc aatcaaccg atggggcca ggtcctgga 
      ggtgcacca ggtggctgg atgaatatg ccgcaagga 
      caagtcaac tgccctccc ggattggaa taccttact 
      atgattgac caattgcta gtccaccaa aaagtcgaa 
      ttattggaa gctctcact ggattcgaa accaaaaat 
      aaatttact gtgaaaaac agcttaggc caaaaagta 
      tattacgcc gtcgaagac tccgattgt ttaactcga 
      aactgttgc ggtccaatt cggccgttc gacatgaac 
      attttagac aattacaaa aacgaagtg attcatttg 
      caccgtcct ttagcgtgt gattcttgt tgtttcccg 
      tgctgcctc caaagcata gaagtttca gcgcctcct 
      ggcacagtt gtaggaagt gtggagcag gaatggagc 
      atcttttgc ccaagtttc gcgattaaa aatccaagt 
      ggcgaaacg gttctccga atagaagga ccgttttgt 
      acgttcagt atttgtgga gacgttgaa tttaagata 
      atgtctgct gatggtaac acacaagtg ggaaaaatt 
      tccaaacag tggtcgggt cttatcaga gaaatgttt 
      accgatact gattatttt gggataacg tttccaatg 
      gacttggac gtaagaatg aaagctgtt atgctagga 
      gcctgcttt ttaattgat gctatgttc tttgaaaag 
      tcgagaaac aaagaagag gattctcca ggcatgctt 
      taa
    mRNA sequence
    • >TC001746 RA
      gcaatttcc gaccacaaa tttcagaaa ttcttgcgt 
      cctatttgc acccatcag taaacaatt ttaagtatt 
      acgtaccaa ttagtgtgt ttaactatc cgactgtct 
      taattagcc ccaaaaaca aataactca gcatgaccc 
      cgaggctaa cagagtgac tcataattt tgacagctt 
      ttcagtaaa caaaaaatt tttagagat gtcgtaccc 
      tccacctgg tggacagca ccctgggcc ttaccctac 
      accctacaa tggtcagta tccaccgat gcccccggg 
      tcagggcta tggaacgcc gccgccgcc cggctatgg 
      cccccctcc ggggtacgg accgccgcc tcaggggta 
      ccccccagg gcagtaccc acccccagg gcaataccc 
      gcccccagg acactatgg gcctccacc agtgatcaa 
      tcaaccgat ggggccagg tcctggagg tgcaccagg 
      tggctggat gaatatgcc gcaaggaca agtcaactg 
      ccctcccgg attggaata ccttactat gattgacca 
      attgctagt ccaccaaaa agtcgaatt attggaagc 
      tctcactgg attcgaaac caaaaataa atttactgt 
      gaaaaacag cttaggcca aaaagtata ttacgccgt 
      cgaagactc cgattgttt aactcgaaa ctgttgcgg 
      tccaattcg gccgttcga catgaacat tttagacaa 
      ttacaaaaa cgaagtgat tcatttgca ccgtccttt 
      agcgtgtga ttcttgttg tttcccgtg ctgcctcca 
      aagcataga agtttcagc gcctcctgg cacagttgt 
      aggaagtgt ggagcagga atggagcat cttttgccc 
      aagtttcgc gattaaaaa tccaagtgg cgaaacggt 
      tctccgaat agaaggacc gttttgtac gttcagtat 
      ttgtggaga cgttgaatt taagataat gtctgctga 
      tggtaacac acaagtggg aaaaatttc caaacagtg 
      gtcgggtct tatcagaga aatgtttac cgatactga 
      ttattttgg gataacgtt tccaatgga cttggacgt 
      aagaatgaa agctgttat gctaggagc ctgcttttt 
      aattgatgc tatgttctt tgaaaagtc gagaaacaa 
      agaagagga ttctccagg catgcttta atttttgtg 
      catttatgt gattgtgtg gttaatttt tattttaag 
      cgtcgttgc acaatgtat tttttattg ttgaattta 
      taaagacgt gtcaattta atattatta actgatgct 
      tagaaaatg gccttcccg actataaat taatatatt 
      atatatatt cttaatact ataactaac tgtttacat 
      attatattc tcgtaataa ttgtacaat ttaccaata 
      taacgttac cattagt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY