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Gene information
General information
TC001745 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8099648..8103765
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001745
    iBeetle screen iB_03245

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Fertility
    vitellogenic egg chamber late decreased - Number of animals showing the phenotype: 4

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001745 RA
      MFPQLSDIG TIWNRLFDH KSFLCGEIH FTLQEFELK 
      RGETEVDNL FKVIENITD IKDTEIGRF KQTVDQVLP 
      QTNNELQQA LSVCSCFAE LENKFKECD HLEAAKQQR 
      KEDWESFMD YITTTYSEI NSLTESVAN ILYKNKVKQ 
      DSNSKPADF KYLLTGEDR QFIKSLKLN SKIDITKLP 
      LSEEAIIHK NDKLLNLQD LHWLYDYIQ KENEGKADK 
      KYLHILLEG SEIILPKNS EIPRNAELE KRCERLRAS 
      QQNKAYNNM TKNVDNVRR KHPEDTISY QMKQMNRHL 
      IAIGQFIIS VLAGFAFGF IGLELLLGS LDFGFRLLL 
      GIICALTIA LAELYFLAK KLNEDLKFE TVAEAKKKG 
      TKLD
    CDS sequence
    • >TC001745 RA
      atgtttcca caactttca gatattgga acaatttgg 
      aatcgactg tttgaccac aagagcttt ctatgcgga 
      gaaattcat ttcacgtta caggaattc gagctcaaa 
      aggggtgaa acagaagtg gataatttg ttcaaagtt 
      atagaaaac atcaccgat attaaagac accgagatt 
      ggtcgcttc aagcagact gttgatcaa gtgctacct 
      caaactaac aacgaattg caacaagcg ttaagtgtg 
      tgcagctgc tttgctgag ttggaaaac aaattcaaa 
      gagtgtgat catttggag gcagctaag caacagaga 
      aaagaggat tgggaaagt tttatggac tatattacc 
      acaacatac tccgaaatc aattccctg accgaaagt 
      gtagctaac attctttac aaaaacaaa gtgaagcaa 
      gattcgaat agtaaacca gcagatttt aaatatttg 
      ttaactgga gaagatcgc cagtttata aaatcgcta 
      aaactcaat tctaaaatt gacattaca aagttgcct 
      ttatccgaa gaagcaata attcacaag aatgataag 
      ctcttgaat ttgcaagat ctgcattgg ttgtacgat 
      tacatccaa aaagaaaat gagggtaaa gcagacaag 
      aaatacctt cacatactt cttgaaggt tcagagatt 
      attttgccc aaaaacagt gaaatacca cgcaatgca 
      gaactagaa aaacgttgt gaacgattg agagcctca 
      cagcaaaat aaagcttac aacaacatg acaaaaaac 
      gttgataat gtgaggcga aagcatcca gaagacaca 
      atttcgtac caaatgaaa caaatgaat cgccacttg 
      attgctatt ggtcagttc attatctca gtccttgct 
      ggatttgcg tttggattc attgggttg gaattgctg 
      ctaggaagt ctggatttt ggatttaga ctgctttta 
      gggattatt tgtgcgtta actatagca ctcgccgaa 
      ttgtacttc ttagctaag aaacttaac gaagatttg 
      aagtttgaa accgtcgct gaagctaag aaaaaaggg 
      acgaaactg gattaa
    mRNA sequence
    • >TC001745 RA
      tcaaaacaa aacaaaata aatcaaaaa tcgaaaagt 
      agcatcctg ttttcaatc attttatct ctaattaat 
      acagaaaca ctatttttt tataattga cgtcatatc 
      agaggattt aggtcggca accaataaa acgcaagct 
      gtaagtttg tcaactaca ccccgatca tatgatcaa 
      actgaaaat gtctgacat ttcaaaggg tgcggtata 
      aacatcaga aaaaccaaa attatcaaa tgtttccac 
      aactttcag atattggaa caatttgga atcgactgt 
      ttgaccaca agagctttc tatgcggag aaattcatt 
      tcacgttac aggaattcg agctcaaaa ggggtgaaa 
      cagaagtgg ataatttgt tcaaagtta tagaaaaca 
      tcaccgata ttaaagaca ccgagattg gtcgcttca 
      agcagactg ttgatcaag tgctacctc aaactaaca 
      acgaattgc aacaagcgt taagtgtgt gcagctgct 
      ttgctgagt tggaaaaca aattcaaag agtgtgatc 
      atttggagg cagctaagc aacagagaa aagaggatt 
      gggaaagtt ttatggact atattacca caacatact 
      ccgaaatca attccctga ccgaaagtg tagctaaca 
      ttctttaca aaaacaaag tgaagcaag attcgaata 
      gtaaaccag cagatttta aatatttgt taactggag 
      aagatcgcc agtttataa aatcgctaa aactcaatt 
      ctaaaattg acattacaa agttgcctt tatccgaag 
      aagcaataa ttcacaaga atgataagc tcttgaatt 
      tgcaagatc tgcattggt tgtacgatt acatccaaa 
      aagaaaatg agggtaaag cagacaaga aataccttc 
      acatacttc ttgaaggtt cagagatta ttttgccca 
      aaaacagtg aaataccac gcaatgcag aactagaaa 
      aacgttgtg aacgattga gagcctcac agcaaaata 
      aagcttaca acaacatga caaaaaacg ttgataatg 
      tgaggcgaa agcatccag aagacacaa tttcgtacc 
      aaatgaaac aaatgaatc gccacttga ttgctattg 
      gtcagttca ttatctcag tccttgctg gatttgcgt 
      ttggattca ttgggttgg aattgctgc taggaagtc 
      tggattttg gatttagac tgcttttag ggattattt 
      gtgcgttaa ctatagcac tcgccgaat tgtacttct 
      tagctaaga aacttaacg aagatttga agtttgaaa 
      ccgtcgctg aagctaaga aaaaaggga cgaaactgg 
      attaaggga tattgtgat acacgtacc tactgtttg 
      agttattct tttgttcaa taaatttca atttaaact
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY