Status
Please wait ...
Gene information
General information
TC001741 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8183157..8217106
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001741
    Gene ontology

    GO terms for Tribolium

    • {{ goTerm.term }}
      ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}
      {{goTerm.showAll ? '[-]' : '[+]'}}
      Status: {{ goTerm.status }}
      Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
      Reference: {{ goTerm.referenceText }}
      Quotation: {{ goTerm.quotation }}
      Submitter: {{ goTerm.name }}

    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03244

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001741 RA
      METFAEAWV AANSKGAPL SDVATQSQA LALTRTCKT 
      PSPPNTTPA ATGRSSRES AERAATPPP PRTPPESTV 
      SPPSVKEEF DAAKTSTAK SLPVHCIVE AICTLDENR 
      ALQHGAWRR RPMVETDSY VIIPVGTPF HSLVQAALL 
      RLGYSADSA AAAKGSVVI KNWKALNFE QISDDPLVT 
      VGDILGELT TIATLRIQV FRGRPGTFN DIKDKLLRF 
      LLLQSHGLL ISSGCPLDE IILSQICRT SPNCAPIPE 
      ISEEIRRKF DQWWSTQFS PQSPASRSQ FGGSFSNQY 
      SVPPQASKS SERTLSESG HPAMQTVHN QFPTQKTRM 
      RTSFDPELE LPKLQRWFT ENQHPSRQQ IQQYVKELN 
      SLESRRGRK PLDVNNVVY WFKNARAAQ KRAEIRNVT 
      PGLPCHLAV NGYSNHSPT NGTGFLISD GYLNSERLA 
      DHRLKNSLG ARRGPQASH TSDEFSNAG SDLEDEDMN 
      EVQPSSPTG PLSLTTTRK HDSRENSPR PSTPPPPPN 
      IQGELCKVE PKDEPINYH SPGDHINNN KINLSDMEE 
      DDLEAENDN KSHFEGSNL NSPASRQIT QRYCNSPEL 
      RTPSDGIER LAAGFPLVP NSMFSHSIM YMSHYIPGL 
      SHTATSPPG SSALGMSGL TADERRKRN RTFIDPVTE 
      VPRLEQWFS LNTHPSHNL ILKYTEELN CMPYRQKFP 
      RLEPKNVQF WFKNRRAKC KRLKMSLFD NQPAGQYHH 
      NERD
    CDS sequence
    • >TC001741 RA
      atggaaacg tttgctgaa gcctgggtt gcagccaac 
      tccaagggt gctcctctg tcagacgtt gccactcag 
      agccaggcg ctagcgctg acgaggacg tgcaaaacg 
      ccgtctcct ccaaacacg acacccgct gccaccggc 
      cgttccagt cgagagtcg gccgagcgc gctgccacc 
      ccgccgccc ccgcggacg cctccagag agcaccgtg 
      tctccgccg tccgtgaaa gaggagttc gacgccgcc 
      aaaacctcc acagccaag tccctccct gttcattgc 
      atcgttgaa gcgatttgc accttggat gagaatcga 
      gcgctacaa cacggagct tggagacga agacctatg 
      gttgaaact gatagctac gtgataatt ccagttgga 
      acccctttt cacagcctc gtgcaagca gcccttctt 
      cgattaggc tattccgct gacagtgca gctgccgcg 
      aaaggttcc gtggttatc aaaaattgg aaagcactt 
      aattttgag caaatatcc gatgatcct ctcgtaact 
      gttggtgac atattaggg gaattaact acgatagcc 
      acgctgagg atccaagtg tttagagga agaccgggc 
      acgttcaac gacattaag gataaactt ttgcgtttc 
      ctgttgctg caatcacat ggattacta atctcttca 
      ggatgtcca ctcgacgag ataatactt tcgcaaatt 
      tgtcgcacg tcaccgaat tgcgccccg attccggaa 
      atatcggaa gaaatccga cgaaaattc gaccagtgg 
      tggtcaaca cagttcagt ccgcagtca ccagcaagc 
      cgaagtcaa ttcggtggt tccttctca aaccaatat 
      tcagtacct ccccaagcg tccaaatcc tccgaacgc 
      acattatcc gaatccggc catccagca atgcaaaca 
      gttcacaat caattcccc acccaaaaa acccgaatg 
      cggaccagt ttcgacccg gaacttgaa ctccccaag 
      ttgcaacgt tggttcacc gaaaatcaa caccccagc 
      cggcagcaa atccaacaa tacgtgaag gaactcaac 
      agtcttgag tccagaagg ggccgcaaa ccgctggac 
      gtcaacaat gtagtctac tggttcaaa aacgcaaga 
      gctgctcaa aaacgcgcg gaaatcaga aacgtcaca 
      ccaggtctg ccatgccat ctcgccgta aacggttac 
      agcaaccat agccctacc aacggtacc ggatttctc 
      atttctgat ggttatttg aactcggaa cgcttagca 
      gatcacaga ttgaaaaac agtctaggg gcgaggcgc 
      gggccgcaa gcgtcgcac acttctgat gaattttca 
      aacgcagga tctgattta gaagatgag gatatgaac 
      gaagtgcaa ccttctagt cccacaggg cccttgtcg 
      ctcactaca acgcggaaa cacgactcc agagagaat 
      agtccgcgt ccctcaaca cccccgcct ccaccaaat 
      atacaagga gagttgtgc aaggtggaa ccaaaagac 
      gagccgatt aactaccac tcgcccggc gaccacata 
      aacaacaac aaaatcaat ctttccgac atggaggag 
      gacgatctg gaagccgaa aatgacaat aaaagtcat 
      ttcgaaggg agcaatttg aactcaccg gcaagtagg 
      cagataact cagcgctac tgcaactct ccagagttg 
      cgcactccc agtgacggt attgaaaga ctagctgca 
      ggttttcct cttgtgcca aactcgatg ttcagccat 
      agcataatg tacatgagc cactatatc ccaggttta 
      tctcacacc gcgacctct ccaccgggt tccagtgct 
      ttagggatg tcgggtttg accgctgac gagcggcga 
      aaacgcaat cgtacattt atagacccc gttacggaa 
      gtaccgaga ttagaacaa tggttttca ctcaacact 
      cacccttcc cacaatctg atattaaag tataccgaa 
      gaattgaac tgtatgcct tatcgtcaa aaatttccc 
      cgattggag ccaaagaac gttcagttt tggttcaaa 
      aatcgacgg gccaagtgc aagagattg aaaatgtca 
      ctgttcgat aatcaaccc gcgggacag tatcaccat 
      aacgagcgc gattaa
    mRNA sequence
    • >TC001741 RA
      ctgaattga agattgaga cgtcctgat ctggttggc 
      tagtgggcc ggccatatt cattaagaa aaagtgtgc 
      agttgatcg ttttttaaa gcatagcgt gtttgcgca 
      aggggtggc gcgttctac ccctatctt gcgctcgca 
      ctcgcgact caggagcga ctcagtcag tcgttgccg 
      ggcgtccgt atcgatagg ttgccgtgc ttcagccgg 
      ctctgagcg ctctgtcac gttgccggt tcacgttgc 
      cgtcatgtt gctctaatt cggtttacc taaattcga 
      acctatgac gatggactt tcagagtgc gatggaaac 
      gtttgctga agcctgggt tgcagccaa ctccaaggg 
      tgctcctct gtcagacgt tgccactca gagccaggc 
      gctagcgct gacgaggac gtgcaaaac gccgtctcc 
      tccaaacac gacacccgc tgccaccgg ccgttccag 
      tcgagagtc ggccgagcg cgctgccac cccgccgcc 
      cccgcggac gcctccaga gagcaccgt gtctccgcc 
      gtccgtgaa agaggagtt cgacgccgc caaaacctc 
      cacagccaa gtccctccc tgttcattg catcgttga 
      agcgatttg caccttgga tgagaatcg agcgctaca 
      acacggagc ttggagacg aagacctat ggttgaaac 
      tgatagcta cgtgataat tccagttgg aaccccttt 
      tcacagcct cgtgcaagc agcccttct tcgattagg 
      ctattccgc tgacagtgc agctgccgc gaaaggttc 
      cgtggttat caaaaattg gaaagcact taattttga 
      gcaaatatc cgatgatcc tctcgtaac tgttggtga 
      catattagg ggaattaac tacgatagc cacgctgag 
      gatccaagt gtttagagg aagaccggg cacgttcaa 
      cgacattaa ggataaact tttgcgttt cctgttgct 
      gcaatcaca tggattact aatctcttc aggatgtcc 
      actcgacga gataatact ttcgcaaat ttgtcgcac 
      gtcaccgaa ttgcgcccc gattccgga aatatcgga 
      agaaatccg acgaaaatt cgaccagtg gtggtcaac 
      acagttcag tccgcagtc accagcaag ccgaagtca 
      attcggtgg ttccttctc aaaccaata ttcagtacc 
      tccccaagc gtccaaatc ctccgaacg cacattatc 
      cgaatccgg ccatccagc aatgcaaac agttcacaa 
      tcaattccc cacccaaaa aacccgaat gcggaccag 
      tttcgaccc ggaacttga actccccaa gttgcaacg 
      ttggttcac cgaaaatca acaccccag ccggcagca 
      aatccaaca atacgtgaa ggaactcaa cagtcttga 
      gtccagaag gggccgcaa accgctgga cgtcaacaa 
      tgtagtcta ctggttcaa aaacgcaag agctgctca 
      aaaacgcgc ggaaatcag aaacgtcac accaggtct 
      gccatgcca tctcgccgt aaacggtta cagcaacca 
      tagccctac caacggtac cggatttct catttctga 
      tggttattt gaactcgga acgcttagc agatcacag 
      attgaaaaa cagtctagg ggcgaggcg cgggccgca 
      agcgtcgca cacttctga tgaattttc aaacgcagg 
      atctgattt agaagatga ggatatgaa cgaagtgca 
      accttctag tcccacagg gcccttgtc gctcactac 
      aacgcggaa acacgactc cagagagaa tagtccgcg 
      tccctcaac acccccgcc tccaccaaa tatacaagg 
      agagttgtg caaggtgga accaaaaga cgagccgat 
      taactacca ctcgcccgg cgaccacat aaacaacaa 
      caaaatcaa tctttccga catggagga ggacgatct 
      ggaagccga aaatgacaa taaaagtca tttcgaagg 
      gagcaattt gaactcacc ggcaagtag gcagataac 
      tcagcgcta ctgcaactc tccagagtt gcgcactcc 
      cagtgacgg tattgaaag actagctgc aggttttcc 
      tcttgtgcc aaactcgat gttcagcca tagcataat 
      gtacatgag ccactatat cccaggttt atctcacac 
      cgcgacctc tccaccggg ttccagtgc tttagggat 
      gtcgggttt gaccgctga cgagcggcg aaaacgcaa 
      tcgtacatt tatagaccc cgttacgga agtaccgag 
      attagaaca atggttttc actcaacac tcacccttc 
      ccacaatct gatattaaa gtataccga agaattgaa 
      ctgtatgcc ttatcgtca aaaatttcc ccgattgga 
      gccaaagaa cgttcagtt ttggttcaa aaatcgacg 
      ggccaagtg caagagatt gaaaatgtc actgttcga 
      taatcaacc cgcgggaca gtatcacca taacgagcg 
      cgattaaaa ctattatta ttattattt ttattgata 
      ttctggtgt gatttttca acctgtact tactgtagg 
      taatgaatg atacattcg atctattac atttttaac 
      aggggttat gacgaattg gcatagaca ataaataat 
      aaaaatatt cgtacccaa tactcgttg gtttccact 
      tttgtttca tgcaaccag tatttcgtt gttagcatg 
      catgtgact agtacctca gcgcaaaat tttattcta 
      tatgttgaa tttgtaata ataattgta ccaaagatt 
      gttaattac atttgtatt gtaagacga atgtttcgt 
      tttgttgat aaataaaga catgtacaa catgttaat 
      cattctgta tcttaataa ttgtcgtgt tgtagccag 
      tggccatgt ctatgtgaa cgcagtgta ttaaaataa 
      atactccta atgtttctt tacgcagtt tgttttttt 
      attataaat atgtatgta aataaaaaa cacaataaa 
      tttttccca agatc
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY