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Gene information
General information
TC001740 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8267954..8273631
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001740
    iBeetle screen iB_03243

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 larval musculature lateral distalirregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    larval musculature lateral potentiallysize decreased -
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001740 RA
      MVHPSELFR TNSNEERTF SAPRNVTLE NQFDCGLTN 
      LPILNRCCN LERLATPKV FLAFLSLVG FLQGITIKY 
      FRETAKFWG DHYNISQNN IDWLIYTNE FFVGLCALL 
      VAYYGNRIH RISWIGGLT IFQAISSGI LVIPEVYRK 
      NYNETKEMP GPTLCSKNG TTLGSEIID QIETNYIAL 
      VILCVFQMF FAMVSVSFI SHGITYLDD NTSYRTSPR 
      YIAIALACK ELGQQAGLY LSWIPTVST ISQIFVHPV 
      WLSIASVTF VSGVLISMF PKYMPSTLI RAMSASIVD 
      LASGHQTVR RKPTNDGFF KTLFRLLKN PILMVNISS 
      IMIMESGLI NFNIFEKYF NQSRFQISY DNDTSYTQI 
      TTNILKQPL VAVSMIACG LLTAKAKHS RSLAKWNIA 
      AIILVVLFF GSTAFLKCN PTTKYEDLL DKNMCKRCN 
      CTFDSFEPV CIEDKTYYS PCCAGCKGV TTMNSRKIF 
      TSCIAGMKA DEGPCNQSE CDLFYALSQ ANRVITTAL 
      LASTIITNI IINMRCILP RDKAMALGL EATFSSLVP 
      YLPVKAIYG AVAEHYCIY RENNVCKLY SKSYATFLS 
      LCSMGFMIL SLLLAVVVL ILLKLTPLD YVDEDEDDV 
      PVELEEVNR PVEGDRLMG EEGTDEVDS SVPRKTGSA 
      NSVESMPES LLDMENVGD DHDSIDSAP RNRILESNL
    CDS sequence
    • >TC001740 RA
      atggtccat ccgagtgaa ttatttcga acaaattca 
      aacgaggaa agaactttt tcggccccg cgaaatgta 
      acgcttgag aatcaattc gactgcggt ttaacaaat 
      ttaccaatt ttgaaccgc tgttgcaac ttggaaaga 
      ctagccaca ccgaaagtg ttcctagca tttttatca 
      cttgttggg tttttacaa gggatcaca attaagtat 
      ttccgagaa actgcaaaa ttctgggga gaccattac 
      aatatttca caaaataat attgattgg ctaatttac 
      accaatgaa ttttttgtt ggactgtgc gccttgtta 
      gtggcctat tatggtaac aggatacac aggatatca 
      tggattggc ggcttaaca attttccaa gccatttca 
      tccggaatt ttagtaatt cccgaagtt tatcggaag 
      aattacaac gaaacgaaa gagatgcca ggtcctact 
      ctgtgttca aaaaatgga acgacacta ggttcagaa 
      ataattgat caaattgaa accaattac attgcactg 
      gtgattttg tgtgttttc caaatgttc tttgccatg 
      gtttcggtt tcatttata tcacatggg attacttat 
      ttggacgat aacacctca tacaggact agcccacgc 
      tatatagca atagcatta gcttgtaag gaattagga 
      caacaagct ggactatat ctgtcttgg attccaacc 
      gtatcaacg atcagtcaa atttttgtt catccagtt 
      tggctttct atagcttca gtgactttt gtgtcaggt 
      gttttaatt tcaatgttc ccaaaatac atgccttca 
      accttgata agagcaatg tcagcttcg attgttgat 
      cttgcatct gggcaccaa accgttcgg cgaaaacca 
      accaatgat gggtttttc aaaacgctt tttcgccta 
      ctaaaaaac ccaatttta atggtcaat atctcaagc 
      attatgata atggagtcg ggacttatc aatttcaat 
      atttttgag aaatatttt aatcaatcc cgtttccaa 
      atttcttac gacaatgat acgtcctac acccagata 
      acgacaaac atattgaaa caacctctg gtggcagta 
      tctatgatt gcctgcgga ctgctaact gccaaagct 
      aaacattca agatctttg gccaaatgg aacatcgct 
      gcaattata cttgttgta ctattcttc ggaagtacg 
      gcctttttg aagtgtaat cccacgacc aaatatgag 
      gatcttctg gataaaaat atgtgtaaa agatgtaac 
      tgcactttt gatagtttc gaaccggtt tgtatcgaa 
      gataagaca tattattcc ccctgctgt gctggttgt 
      aagggtgtc acaactatg aattccaga aagattttc 
      acaagttgc atcgcggga atgaaagct gacgaaggt 
      ccatgtaat caaagtgaa tgtgacttg ttttacgcc 
      ttgtctcaa gccaatcga gtcattaca acagcatta 
      ctcgcatca acgattata acaaatatt ataattaac 
      atgagatgc attttacca cgggataag gcaatggcg 
      ttaggtctt gaagcgact ttttcaagt ctagttcct 
      tatttaccc gtgaaagca atctacgga gctgtggca 
      gaacactac tgtatttat cgtgaaaat aatgtttgc 
      aagctatac tcgaaaagc tatgccacg tttttatcc 
      ttgtgttcg atgggtttt atgattctt tcgttactt 
      ttggcagtt gttgtttta attcttttg aaattaaca 
      cctctggat tatgtggat gaagatgaa gatgatgtt 
      cccgttgaa cttgaagag gtgaacaga ccggttgaa 
      ggagataga ttaatggga gaagaggga acagatgaa 
      gttgattcg agtgtccct cgaaaaact ggttcggca 
      aattcagtc gagagtatg cctgagtca cttctagac 
      atggaaaat gttggagat gatcatgat tcgatcgat 
      tcggctcct cggaataga atactagag agcaattta 
      tga
    mRNA sequence
    • >TC001740 RA
      tttcaaatg aaaatattt gagtcacac ctagtcgtg 
      cgtttacaa tggtccatc cgagtgaat tatttcgaa 
      caaattcaa acgaggaaa gaacttttt cggccccgc 
      gaaatgtaa cgcttgaga atcaattcg actgcggtt 
      taacaaatt taccaattt tgaaccgct gttgcaact 
      tggaaagac tagccacac cgaaagtgt tcctagcat 
      ttttatcac ttgttgggt ttttacaag ggatcacaa 
      ttaagtatt tccgagaaa ctgcaaaat tctggggag 
      accattaca atatttcac aaaataata ttgattggc 
      taatttaca ccaatgaat tttttgttg gactgtgcg 
      ccttgttag tggcctatt atggtaaca ggatacaca 
      ggatatcat ggattggcg gcttaacaa ttttccaag 
      ccatttcat ccggaattt tagtaattc ccgaagttt 
      atcggaaga attacaacg aaacgaaag agatgccag 
      gtcctactc tgtgttcaa aaaatggaa cgacactag 
      gttcagaaa taattgatc aaattgaaa ccaattaca 
      ttgcactgg tgattttgt gtgttttcc aaatgttct 
      ttgccatgg tttcggttt catttatat cacatggga 
      ttacttatt tggacgata acacctcat acaggacta 
      gcccacgct atatagcaa tagcattag cttgtaagg 
      aattaggac aacaagctg gactatatc tgtcttgga 
      ttccaaccg tatcaacga tcagtcaaa tttttgttc 
      atccagttt ggctttcta tagcttcag tgacttttg 
      tgtcaggtg ttttaattt caatgttcc caaaataca 
      tgccttcaa ccttgataa gagcaatgt cagcttcga 
      ttgttgatc ttgcatctg ggcaccaaa ccgttcggc 
      gaaaaccaa ccaatgatg ggtttttca aaacgcttt 
      ttcgcctac taaaaaacc caattttaa tggtcaata 
      tctcaagca ttatgataa tggagtcgg gacttatca 
      atttcaata tttttgaga aatatttta atcaatccc 
      gtttccaaa tttcttacg acaatgata cgtcctaca 
      cccagataa cgacaaaca tattgaaac aacctctgg 
      tggcagtat ctatgattg cctgcggac tgctaactg 
      ccaaagcta aacattcaa gatctttgg ccaaatgga 
      acatcgctg caattatac ttgttgtac tattcttcg 
      gaagtacgg cctttttga agtgtaatc ccacgacca 
      aatatgagg atcttctgg ataaaaata tgtgtaaaa 
      gatgtaact gcacttttg atagtttcg aaccggttt 
      gtatcgaag ataagacat attattccc cctgctgtg 
      ctggttgta agggtgtca caactatga attccagaa 
      agattttca caagttgca tcgcgggaa tgaaagctg 
      acgaaggtc catgtaatc aaagtgaat gtgacttgt 
      tttacgcct tgtctcaag ccaatcgag tcattacaa 
      cagcattac tcgcatcaa cgattataa caaatatta 
      taattaaca tgagatgca ttttaccac gggataagg 
      caatggcgt taggtcttg aagcgactt tttcaagtc 
      tagttcctt atttacccg tgaaagcaa tctacggag 
      ctgtggcag aacactact gtatttatc gtgaaaata 
      atgtttgca agctatact cgaaaagct atgccacgt 
      ttttatcct tgtgttcga tgggtttta tgattcttt 
      cgttacttt tggcagttg ttgttttaa ttcttttga 
      aattaacac ctctggatt atgtggatg aagatgaag 
      atgatgttc ccgttgaac ttgaagagg tgaacagac 
      cggttgaag gagatagat taatgggag aagagggaa 
      cagatgaag ttgattcga gtgtccctc gaaaaactg 
      gttcggcaa attcagtcg agagtatgc ctgagtcac 
      ttctagaca tggaaaatg ttggagatg atcatgatt 
      cgatcgatt cggctcctc ggaatagaa tactagaga 
      gcaatttat gattgtatt tatttacaa agactaatc 
      aaacgactc gagaggagt taagctaaa tcttcgagt 
      tcaagttca a
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