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Gene information
General information
TC001738 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8333123..8347551
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001738
    iBeetle screen iB_03242

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue potentially present, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 30% - 50%

    embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001738 RA
      MDKTDQKKN SKRHGANES HGTRHISNS SSGVLMVGP 
      NFRVGKKIG CGNFGELRL GKNLYNNEH VAIKMEPMK 
      SKAPQLHLE YRFYKFLGS HEGIPDVYY FGPCGKYNA 
      LVMELLGPS LEDLFDMCD RKFSLKTVL QIAIQLLHR 
      IEYVHSRHL IYRDVKPEN FLIGRSSTK REKIIHIID 
      FGLAKEYVE METNKHIPY REHKSLTGT ARYMSINTH 
      LGKEQSRRD DLEALGHMF MYFLRGSLP WQGLKADTL 
      KERYQKIGD TKRATPIEV LCEGHPEEM ATYLRYVRR 
      LDFFETPDY EYLRNLFQN LFNRKGYPD DNEFDWSGK 
      TMSTPVGSL QTTAHEVVV SPNRERHIT NKTTSKANA 
      WPEASKQST LGNLTPADR HGSVQVVSS TNGELNADD 
      PTAGHSNTP ITQPQEIEL LDETKCCCF FKRKKKKGV 
      RTK
    • >TC001738 RB
      MDKTDQKKN SKRHGANES HGTRHISNS SSGVLMVGP 
      NFRVGKKIG CGNFGELRL GKNLYNNEH VAIKMEPMK 
      SKAPQLHLE YRFYKFLGS HEGIPDVYY FGPCGKYNA 
      LVMELLGPS LEDLFDMCD RKFSLKTVL QIAIQLLHR 
      IEYVHSRHL IYRDVKPEN FLIGRSSTK REKIIHIID 
      FGLAKEYVE METNKHIPY REHKSLTGT ARYMSINTH 
      LGKEQSRRD DLEALGHMF MYFLRGSLP WQGLKADTL 
      KERYQKIGD TKRATPIEV LCEGHPEEM ATYLRYVRR 
      LDFFETPDY EYLRNLFQN LFNRKGYPD DNEFDWSGK 
      TMSTPVGSL QTTAHEVVV SPNRERHIT NKTTSKANA 
      WPEASKQST LGNLTPADR HGSVQVVSS TNGELNADD 
      PTAGHSNTP ITQPQEIEL LDETKKRIC VWLSFRCCC 
      FFKRKKKKG VRTK
    CDS sequence
    • >TC001738 RA
      atggacaaa accgatcag aaaaagaat tcaaaacgc 
      catggtgcc aacgagtct catgggaca cgccatatc 
      tccaactcc agttctggg gttttaatg gtgggtcca 
      aatttccga gttgggaag aaaataggc tgtggaaac 
      ttcggcgaa ttacgccta ggaaaaaac ctttataat 
      aacgaacat gtagccatt aaaatggaa cccatgaaa 
      tcgaaagct ccgcaatta caccttgag tatagattt 
      tacaagttt ttgggtagt catgaagga ataccagat 
      gtttattat ttcggccct tgtgggaaa tataatgct 
      ctggtgatg gaattactc ggccctagt ttagaggat 
      cttttcgac atgtgtgac cgaaaattt tccttaaag 
      actgtttta caaatagcg atccaactc ttgcacagg 
      attgagtat gtacatagt cgtcacctt atttatcgg 
      gatgttaaa cccgaaaac ttccttatt ggacgaagt 
      agtacaaaa cgcgaaaaa ataattcac attatagat 
      tttggttta gctaaagaa tacgtggaa atggaaaca 
      aataaacat attccgtat agagaacac aaatcgttg 
      actggaaca gcgcgatat atgtcgatc aacacccat 
      ctaggcaag gagcaatct cgtcgtgat gatttggaa 
      gctttgggt catatgttt atgtatttt ttgcgagga 
      tctctccct tggcaagga ctcaaagcc gacacgtta 
      aaggagcga tatcaaaaa attggtgac acgaaacgt 
      gccacacca attgaagtt ctatgtgaa ggacatccg 
      gaagaaatg gcaacttat ctgagatac gtacgacga 
      ttggatttt ttcgaaact ccagactat gaatatctg 
      aggaatcta ttccaaaat ttattcaac cgtaaaggt 
      tatcccgac gataacgaa tttgattgg tcgggaaag 
      acaatgagt accccagtc ggttctcta caaaccaca 
      gcacacgaa gtagtagtt tctccaaat agggaaagg 
      catattaca aataagaca actagcaaa gcaaatgca 
      tggcctgaa gcttcgaag cagagtaca ttgggcaac 
      ttgacacca gctgaccgt cacggttct gtacaggtt 
      gtcagttcg accaatggt gaattaaac gctgatgac 
      cccacagct ggacacagc aacactcct ataacacaa 
      cctcaggaa attgaactt cttgacgaa accaagtgc 
      tgctgtttc tttaagaga aagaagaag aaaggtgta 
      cgcactaaa tga
    • >TC001738 RB
      atggacaaa accgatcag aaaaagaat tcaaaacgc 
      catggtgcc aacgagtct catgggaca cgccatatc 
      tccaactcc agttctggg gttttaatg gtgggtcca 
      aatttccga gttgggaag aaaataggc tgtggaaac 
      ttcggcgaa ttacgccta ggaaaaaac ctttataat 
      aacgaacat gtagccatt aaaatggaa cccatgaaa 
      tcgaaagct ccgcaatta caccttgag tatagattt 
      tacaagttt ttgggtagt catgaagga ataccagat 
      gtttattat ttcggccct tgtgggaaa tataatgct 
      ctggtgatg gaattactc ggccctagt ttagaggat 
      cttttcgac atgtgtgac cgaaaattt tccttaaag 
      actgtttta caaatagcg atccaactc ttgcacagg 
      attgagtat gtacatagt cgtcacctt atttatcgg 
      gatgttaaa cccgaaaac ttccttatt ggacgaagt 
      agtacaaaa cgcgaaaaa ataattcac attatagat 
      tttggttta gctaaagaa tacgtggaa atggaaaca 
      aataaacat attccgtat agagaacac aaatcgttg 
      actggaaca gcgcgatat atgtcgatc aacacccat 
      ctaggcaag gagcaatct cgtcgtgat gatttggaa 
      gctttgggt catatgttt atgtatttt ttgcgagga 
      tctctccct tggcaagga ctcaaagcc gacacgtta 
      aaggagcga tatcaaaaa attggtgac acgaaacgt 
      gccacacca attgaagtt ctatgtgaa ggacatccg 
      gaagaaatg gcaacttat ctgagatac gtacgacga 
      ttggatttt ttcgaaact ccagactat gaatatctg 
      aggaatcta ttccaaaat ttattcaac cgtaaaggt 
      tatcccgac gataacgaa tttgattgg tcgggaaag 
      acaatgagt accccagtc ggttctcta caaaccaca 
      gcacacgaa gtagtagtt tctccaaat agggaaagg 
      catattaca aataagaca actagcaaa gcaaatgca 
      tggcctgaa gcttcgaag cagagtaca ttgggcaac 
      ttgacacca gctgaccgt cacggttct gtacaggtt 
      gtcagttcg accaatggt gaattaaac gctgatgac 
      cccacagct ggacacagc aacactcct ataacacaa 
      cctcaggaa attgaactt cttgacgaa accaaaaaa 
      cgaatatgt gtgtggttg tcttttagg tgctgctgt 
      ttctttaag agaaagaag aagaaaggt gtacgcact 
      aaatga
    mRNA sequence
    • >TC001738 RA
      atggtacag cacactctc cgtctccgg gacgagtgc 
      gtgaaaatg gtgaattcc taggttctg aaagcaagt 
      ctagacttg tccatgtag tgatatccg actaaagtt 
      ttatggtgt gctcctgta agtgtttga gtggcccaa 
      atcatcaaa ttctctaaa tgacgttga ccgggtctg 
      agtcagtaa tggccgtca gacctaacc acggtagaa 
      gatattgaa atgacattt cacaacatc ccatcagga 
      ggaatattc aaagaaaaa taattgtta gtgcaattg 
      tacgtcaga acgcttcgc taggctacg gaaatttgt 
      gtaatacac tgtgatact aacaaaatg tgatataaa 
      cgactgaaa tgaatttat aaaagtgca tcctggccc 
      cgaaaacgt ttctatgca gtgctttcc ttggatgaa 
      ccgcacgat tcaactgaa acaactgtt aaaataact 
      attttgagg gtataacca tggacaaaa ccgatcaga 
      aaaagaatt caaaacgcc atggtgcca acgagtctc 
      atgggacac gccatatct ccaactcca gttctgggg 
      ttttaatgg tgggtccaa atttccgag ttgggaaga 
      aaataggct gtggaaact tcggcgaat tacgcctag 
      gaaaaaacc tttataata acgaacatg tagccatta 
      aaatggaac ccatgaaat cgaaagctc cgcaattac 
      accttgagt atagatttt acaagtttt tgggtagtc 
      atgaaggaa taccagatg tttattatt tcggccctt 
      gtgggaaat ataatgctc tggtgatgg aattactcg 
      gccctagtt tagaggatc ttttcgaca tgtgtgacc 
      gaaaatttt ccttaaaga ctgttttac aaatagcga 
      tccaactct tgcacagga ttgagtatg tacatagtc 
      gtcacctta tttatcggg atgttaaac ccgaaaact 
      tccttattg gacgaagta gtacaaaac gcgaaaaaa 
      taattcaca ttatagatt ttggtttag ctaaagaat 
      acgtggaaa tggaaacaa ataaacata ttccgtata 
      gagaacaca aatcgttga ctggaacag cgcgatata 
      tgtcgatca acacccatc taggcaagg agcaatctc 
      gtcgtgatg atttggaag ctttgggtc atatgttta 
      tgtattttt tgcgaggat ctctccctt ggcaaggac 
      tcaaagccg acacgttaa aggagcgat atcaaaaaa 
      ttggtgaca cgaaacgtg ccacaccaa ttgaagttc 
      tatgtgaag gacatccgg aagaaatgg caacttatc 
      tgagatacg tacgacgat tggattttt tcgaaactc 
      cagactatg aatatctga ggaatctat tccaaaatt 
      tattcaacc gtaaaggtt atcccgacg ataacgaat 
      ttgattggt cgggaaaga caatgagta ccccagtcg 
      gttctctac aaaccacag cacacgaag tagtagttt 
      ctccaaata gggaaaggc atattacaa ataagacaa 
      ctagcaaag caaatgcat ggcctgaag cttcgaagc 
      agagtacat tgggcaact tgacaccag ctgaccgtc 
      acggttctg tacaggttg tcagttcga ccaatggtg 
      aattaaacg ctgatgacc ccacagctg gacacagca 
      acactccta taacacaac ctcaggaaa ttgaacttc 
      ttgacgaaa ccaagtgct gctgtttct ttaagagaa 
      agaagaaga aaggtgtac gcactaaat gactacaat 
      acagcgaga cagaccaac atctcacac tgttaccgt 
      gtggacata gtagtgcag caacactta attgactat 
      gtttaaatg aagactttt gtcgattgt tacgttgta 
      cggtttaat agctaattt aggttagcc ttaatttaa 
      aagtaagtt agtttaagt cataccata tctgtgaac 
      gtctacgga agccttact cgtcctcgg ttctgtact 
      tttttccac ttgtatttt aatcttacc tagtttgtc 
      gtatttatt atttaccaa atactacgt tgtatgaat 
      tatttgact tagtgtagt gtgtgttct agtaaacat 
      ttaacatat tattttatc tatgaaagg agagaacgt 
      gttgatttt tgcgagtaa ggccgagtt ttagtatgt 
      ttgataggg gatgattac tgaacaatt gaggtgtaa 
      ataatcaaa cagcctttc accgcattt acaattgtt 
      tggaacacg cgtcattct taacgccaa attatcatt 
      gctattttt taagcttgc tcttttgtt tgtatatag 
      taatgttcg catgttgaa cgttaaaat atttaattc 
      gcagtcttt attcttttt atatacaca atatatttg 
      gcacgttca atatgggga cctgaagga attttaacg 
      taaagttgt gatttaatc gttgtataa atttgcttt 
      gttgttgat ctcaagtaa aaactgatc gactgctta 
      actcttctt tgaaccaca agcaaccta caagttgat 
      aacttgtgg atctgcttt ataccaaca atttgattt 
      tatttcttt tgtggctcg tataaatat agaatgtta 
      gaacac
    • >TC001738 RB
      atggtacag cacactctc cgtctccgg gacgagtgc 
      gtgaaaatg gtgaattcc taggttctg aaagcaagt 
      ctagacttg tccatgtag tgatatccg actaaagtt 
      ttatggtgt gctcctgta agtgtttga gtggcccaa 
      atcatcaaa ttctctaaa tgacgttga ccgggtctg 
      agtcagtaa tggccgtca gacctaacc acggtagaa 
      gatattgaa atgacattt cacaacatc ccatcagga 
      ggaatattc aaagaaaaa taattgtta gtgcaattg 
      tacgtcaga acgcttcgc taggctacg gaaatttgt 
      gtaatacac tgtgatact aacaaaatg tgatataaa 
      cgactgaaa tgaatttat aaaagtgca tcctggccc 
      cgaaaacgt ttctatgca gtgctttcc ttggatgaa 
      ccgcacgat tcaactgaa acaactgtt aaaataact 
      attttgagg gtataacca tggacaaaa ccgatcaga 
      aaaagaatt caaaacgcc atggtgcca acgagtctc 
      atgggacac gccatatct ccaactcca gttctgggg 
      ttttaatgg tgggtccaa atttccgag ttgggaaga 
      aaataggct gtggaaact tcggcgaat tacgcctag 
      gaaaaaacc tttataata acgaacatg tagccatta 
      aaatggaac ccatgaaat cgaaagctc cgcaattac 
      accttgagt atagatttt acaagtttt tgggtagtc 
      atgaaggaa taccagatg tttattatt tcggccctt 
      gtgggaaat ataatgctc tggtgatgg aattactcg 
      gccctagtt tagaggatc ttttcgaca tgtgtgacc 
      gaaaatttt ccttaaaga ctgttttac aaatagcga 
      tccaactct tgcacagga ttgagtatg tacatagtc 
      gtcacctta tttatcggg atgttaaac ccgaaaact 
      tccttattg gacgaagta gtacaaaac gcgaaaaaa 
      taattcaca ttatagatt ttggtttag ctaaagaat 
      acgtggaaa tggaaacaa ataaacata ttccgtata 
      gagaacaca aatcgttga ctggaacag cgcgatata 
      tgtcgatca acacccatc taggcaagg agcaatctc 
      gtcgtgatg atttggaag ctttgggtc atatgttta 
      tgtattttt tgcgaggat ctctccctt ggcaaggac 
      tcaaagccg acacgttaa aggagcgat atcaaaaaa 
      ttggtgaca cgaaacgtg ccacaccaa ttgaagttc 
      tatgtgaag gacatccgg aagaaatgg caacttatc 
      tgagatacg tacgacgat tggattttt tcgaaactc 
      cagactatg aatatctga ggaatctat tccaaaatt 
      tattcaacc gtaaaggtt atcccgacg ataacgaat 
      ttgattggt cgggaaaga caatgagta ccccagtcg 
      gttctctac aaaccacag cacacgaag tagtagttt 
      ctccaaata gggaaaggc atattacaa ataagacaa 
      ctagcaaag caaatgcat ggcctgaag cttcgaagc 
      agagtacat tgggcaact tgacaccag ctgaccgtc 
      acggttctg tacaggttg tcagttcga ccaatggtg 
      aattaaacg ctgatgacc ccacagctg gacacagca 
      acactccta taacacaac ctcaggaaa ttgaacttc 
      ttgacgaaa ccaaaaaac gaatatgtg tgtggttgt 
      cttttaggt gctgctgtt tctttaaga gaaagaaga 
      agaaaggtg tacgcacta aatgactac aatacagcg 
      agacagacc aacatctca cactgttac cgtgtggac 
      atagtagtg cagcaacac ttaattgac tatgtttaa 
      atgaagact tttgtcgat tgttacgtt gtacggttt 
      aatagctaa tttaggtta gccttaatt taaaagtaa 
      gttagttta agtcatacc atatctgtg aacgtctac 
      ggaagcctt actcgtcct cggttctgt acttttttc 
      cacttgtat tttaatctt acctagttt gtcgtattt 
      attatttac caaatacta cgttgtatg aattatttg 
      acttagtgt agtgtgtgt tctagtaaa catttaaca 
      tattatttt atctatgaa aggagagaa cgtgttgat 
      ttttgcgag taaggccga gttttagta tgtttgata 
      ggggatgat tactgaaca attgaggtg taaataatc 
      aaacagcct ttcaccgca tttacaatt gtttggaac 
      acgcgtcat tcttaacgc caaattatc attgctatt 
      ttttaagct tgctctttt gtttgtata tagtaatgt 
      tcgcatgtt gaacgttaa aatatttaa ttcgcagtc 
      tttattctt tttatatac acaatatat ttggcacgt 
      tcaatatgg ggacctgaa ggaatttta acgtaaagt 
      tgtgattta atcgttgta taaatttgc tttgttgtt 
      gatctcaag taaaaactg atcgactgc ttaactctt 
      ctttgaacc acaagcaac ctacaagtt gataacttg 
      tggatctgc tttatacca acaatttga ttttatttc 
      ttttgtggc tcgtataaa tatagaatg ttagaacac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY