Status
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Gene information
General information
TC001737 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8476315..8483406
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001737
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    Publish name
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03241

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001737 RA
      MSLLYRAKF GTDILSVTN PWKCIRNNS NAPPTQARI 
      VIAGAGVVA NSVAYHLVQ NGWSDIIVL EQGKIGSGT 
      SHFGSGTLG LFKPIAHRN LIWYSIKLY QQLQDMGYD 
      IQMRQCGSI NLAQTNDRL IALKRRLAY NIPTGLYCE 
      LLSPNELKD LHPYLRVDD LKGAVWVPE DAVANPRAI 
      CDVLAILAQ QGGAQYFEH TPVDKVLTQ NSTVYGVET 
      PKGVIKCEY FVNCAGMWA RELGLQCSP QVRIPAYPA 
      QHFYATTGR LTKIIDEST PIIRDYDAF TYAREHEGG 
      FMIGWFEEE AKPAFEDSQ VPRDWLRHV KKDFNHFFP 
      LWEQAIRRI PELSNCKKP EIVNSPDNF TPDGRWILG 
      ETAEVTNYF VACGMNGNS LQGAGGIGK AVAEWIIEG 
      EPTQDLLPF NVQRFLDVH NSRQYLQQR IKEIVGRHY 
      SILYPFQCE YKYARKLRC SPLYSVLET KGAVFGIKM 
      AYERALYFD SRYMNGDSK PIMPPGSFF KPKFFDYMK 
      EEYLACREG VGIIDMSSF SKIEIKSDH LEVVNYLQR 
      LCSNDIDIA VGGIVHTGM QNERGGYEN DCMLVRQAD 
      NKYFMVSPT SQQTRIYEW ISRNLPKKS SIDLNDVTS 
      MYTVINVVG PKAHELLNE LSNSDMRLT PFSYKKVNM 
      GYASDVMVM SFTHTGEPG YCLYVPSEY ALHIYYKLM 
      TVGRDYGVR DVGTLTQRF MRIERFIPF WAEELSSFT 
      TPFEAGNGY SVRLDKREN FIGKPALQQ QKENGVTKQ 
      LVFFHLNDI DPDKDIWPW GGEPLYRNN EFVGTVTSA 
      GYGFSADKL ICLGFIRRP KSSSVKTIT SEYILSKEA 
      KYEIDIAGT RFSIMPYLH APPVTNTVN LGQKYRPTV 
      IRYKSDVTS
    CDS sequence
    • >TC001737 RA
      atgtcactc ctgtataga gcaaaattt ggcactgat 
      attttaagt gttacaaac ccgtggaaa tgtatcaga 
      aacaactca aatgcacca ccaactcaa gcaagaata 
      gtgatcgca ggggctgga gttgtcgca aactctgtg 
      gcctatcat ttggttcag aatggttgg agcgatatt 
      attgtccta gaacagggc aaaatcggc agtggaacg 
      tctcatttc ggttccggg accttgggt ttgttcaaa 
      ccaatcgct cacagaaat ctgatctgg tacagtatt 
      aaattatac caacaattg caagacatg ggctatgac 
      atccagatg aggcagtgc gggagcatt aatttagcc 
      caaactaat gatagactg atagcttta aaaagacga 
      ttggcttat aatattccc acagggtta tactgtgaa 
      ttgcttagt ccaaatgaa cttaaagac ttgcaccct 
      tacttacga gtagatgac ttaaaggga gctgtttgg 
      gtccctgaa gacgcagta gctaacccc agagctatc 
      tgtgatgtc ctggcaata ttagcccaa caagggggc 
      gcgcaatac ttcgaacat acacctgta gacaaagta 
      cttacccaa aactcaact gtttatggc gtggagact 
      ccgaaaggg gttatcaaa tgtgaatat tttgtgaac 
      tgtgctggg atgtgggct cgggaattg gggctgcag 
      tgttcgcca caagtgcgc atccctgct tatccggca 
      caacacttc tatgcaacc acggggcgt ctcaccaaa 
      attattgac gaatcaaca ccaataata agagattat 
      gacgctttc acctacgca cgcgaacac gaagggggt 
      tttatgata ggatggttt gaagaggaa gctaagccc 
      gcattcgag gatagccaa gtgcctaga gattggttg 
      agacacgtt aaaaaggat tttaatcac ttttttcct 
      ttatgggaa caagccatc cgtcggatc cccgaacta 
      tccaactgc aaaaaaccg gaaatcgtt aactctcct 
      gataatttc actccggat ggtagatgg attctgggt 
      gaaactgct gaagtgact aattatttt gttgcctgt 
      ggcatgaac ggtaattct ttacagggt gcgggaggg 
      ataggtaaa gctgtagct gaatggatt atcgaggga 
      gagcccact caagatctt ttacctttc aatgtacaa 
      agattttta gatgttcat aacagtaga cagtactta 
      caacaaaga attaaagaa attgtgggc aggcattac 
      tcaattcta tatcccttt cagtgtgaa tataaatat 
      gcgaggaaa cttcgctgc tctcccctg tacagtgtg 
      ctagaaaca aaaggggca gtgtttgga attaagatg 
      gcatatgaa agggctctt tactttgat tcaagatac 
      atgaatggt gactctaaa ccaattatg cctccgggt 
      agttttttt aaaccgaag tttttcgat tacatgaaa 
      gaagagtat ttggcatgt cgggaaggc gtaggaatt 
      attgatatg tcttcattt tcaaaaatt gaaattaag 
      tcagaccat cttgaagtc gtaaattat ctccaacgg 
      ttgtgttcc aatgacatt gatattgcc gtgggagga 
      atagtacat acagggatg caaaatgag agaggcggc 
      tatgaaaat gactgcatg ttagtgaga caagcggat 
      aacaagtac tttatggta tcaccaact agtcaacaa 
      acgcgaatc tacgaatgg atctcgcgt aatttaccg 
      aaaaaatca tcaattgat ttaaacgat gttacatca 
      atgtacaca gtaatcaat gttgtgggt cctaaagca 
      cacgaattg ctcaacgaa ttgtccaac agtgatatg 
      cgactaaca cctttctct tacaaaaaa gtaaacatg 
      ggctatgct tccgacgtc atggttatg tcatttaca 
      cacactgga gaaccagga tactgtttg tacgtccct 
      tcagaatac gctttacac atatactac aaattaatg 
      acagtgggt cgcgactat ggtgtgcga gatgttggg 
      actttaact caacgtttt atgcgaatt gagcgtttt 
      atccctttt tgggcggaa gaattgagc agttttacg 
      acacctttt gaggcagga aacggttac agtgtacga 
      ctggacaaa agggaaaat tttatcgga aaaccagct 
      ctccagcaa cagaaagaa aacggagta actaaacaa 
      cttgtcttc ttccatttg aatgatata gaccccgat 
      aaagatatt tggccgtgg ggtggagaa ccgttgtac 
      cgaaacaat gaatttgtt ggaactgtt acatccgct 
      ggttatgga ttttcagcc gataagttg atttgtttg 
      ggttttatt cgcagacca aaatcgtca agcgttaaa 
      acgattact tccgaatat atactgtcg aaagaggct 
      aagtatgaa atcgatatt gcgggcact cgattttct 
      ataatgcca tatcttcat gccccacct gtcacaaat 
      acggttaat ttaggtcag aagtatcga ccgactgtt 
      attaggtat aaaagtgat gtgacaagt tag
    mRNA sequence
    • >TC001737 RA
      gtggtgtgg cagtgtgtt gttggttgc cgacttgcc 
      tagccacca aatttttat aaaacaatt tgaaaaata 
      gtaatttca gatataaga tgtaacgta cacctcagc 
      actaaaaat taatatttt ttgcattac tgattccca 
      acagcaaaa ataataaat tcaaatgaa gtattgttg 
      ctggtatcc tagattgtc agattcagt ttgaggtta 
      tgtttgtgt atatttttt cgtaaatat taagtaaaa 
      tggtcactg ttacaagac caacattta catgacgct 
      tccaattaa aaatgtcac tcctgtata gagcaaaat 
      ttggcactg atattttaa gtgttacaa acccgtgga 
      aatgtatca gaaacaact caaatgcac caccaactc 
      aagcaagaa tagtgatcg caggggctg gagttgtcg 
      caaactctg tggcctatc atttggttc agaatggtt 
      ggagcgata ttattgtcc tagaacagg gcaaaatcg 
      gcagtggaa cgtctcatt tcggttccg ggaccttgg 
      gtttgttca aaccaatcg ctcacagaa atctgatct 
      ggtacagta ttaaattat accaacaat tgcaagaca 
      tgggctatg acatccaga tgaggcagt gcgggagca 
      ttaatttag cccaaacta atgatagac tgatagctt 
      taaaaagac gattggctt ataatattc ccacagggt 
      tatactgtg aattgctta gtccaaatg aacttaaag 
      acttgcacc cttacttac gagtagatg acttaaagg 
      gagctgttt gggtccctg aagacgcag tagctaacc 
      ccagagcta tctgtgatg tcctggcaa tattagccc 
      aacaagggg gcgcgcaat acttcgaac atacacctg 
      tagacaaag tacttaccc aaaactcaa ctgtttatg 
      gcgtggaga ctccgaaag gggttatca aatgtgaat 
      attttgtga actgtgctg ggatgtggg ctcgggaat 
      tggggctgc agtgttcgc cacaagtgc gcatccctg 
      cttatccgg cacaacact tctatgcaa ccacggggc 
      gtctcacca aaattattg acgaatcaa caccaataa 
      taagagatt atgacgctt tcacctacg cacgcgaac 
      acgaagggg gttttatga taggatggt ttgaagagg 
      aagctaagc ccgcattcg aggatagcc aagtgccta 
      gagattggt tgagacacg ttaaaaagg attttaatc 
      acttttttc ctttatggg aacaagcca tccgtcgga 
      tccccgaac tatccaact gcaaaaaac cggaaatcg 
      ttaactctc ctgataatt tcactccgg atggtagat 
      ggattctgg gtgaaactg ctgaagtga ctaattatt 
      ttgttgcct gtggcatga acggtaatt ctttacagg 
      gtgcgggag ggataggta aagctgtag ctgaatgga 
      ttatcgagg gagagccca ctcaagatc ttttacctt 
      tcaatgtac aaagatttt tagatgttc ataacagta 
      gacagtact tacaacaaa gaattaaag aaattgtgg 
      gcaggcatt actcaattc tatatccct ttcagtgtg 
      aatataaat atgcgagga aacttcgct gctctcccc 
      tgtacagtg tgctagaaa caaaagggg cagtgtttg 
      gaattaaga tggcatatg aaagggctc tttactttg 
      attcaagat acatgaatg gtgactcta aaccaatta 
      tgcctccgg gtagttttt ttaaaccga agtttttcg 
      attacatga aagaagagt atttggcat gtcgggaag 
      gcgtaggaa ttattgata tgtcttcat tttcaaaaa 
      ttgaaatta agtcagacc atcttgaag tcgtaaatt 
      atctccaac ggttgtgtt ccaatgaca ttgatattg 
      ccgtgggag gaatagtac atacaggga tgcaaaatg 
      agagaggcg gctatgaaa atgactgca tgttagtga 
      gacaagcgg ataacaagt actttatgg tatcaccaa 
      ctagtcaac aaacgcgaa tctacgaat ggatctcgc 
      gtaatttac cgaaaaaat catcaattg atttaaacg 
      atgttacat caatgtaca cagtaatca atgttgtgg 
      gtcctaaag cacacgaat tgctcaacg aattgtcca 
      acagtgata tgcgactaa cacctttct cttacaaaa 
      aagtaaaca tgggctatg cttccgacg tcatggtta 
      tgtcattta cacacactg gagaaccag gatactgtt 
      tgtacgtcc cttcagaat acgctttac acatatact 
      acaaattaa tgacagtgg gtcgcgact atggtgtgc 
      gagatgttg ggactttaa ctcaacgtt ttatgcgaa 
      ttgagcgtt ttatccctt tttgggcgg aagaattga 
      gcagtttta cgacacctt ttgaggcag gaaacggtt 
      acagtgtac gactggaca aaagggaaa attttatcg 
      gaaaaccag ctctccagc aacagaaag aaaacggag 
      taactaaac aacttgtct tcttccatt tgaatgata 
      tagaccccg ataaagata tttggccgt ggggtggag 
      aaccgttgt accgaaaca atgaatttg ttggaactg 
      ttacatccg ctggttatg gattttcag ccgataagt 
      tgatttgtt tgggtttta ttcgcagac caaaatcgt 
      caagcgtta aaacgatta cttccgaat atatactgt 
      cgaaagagg ctaagtatg aaatcgata ttgcgggca 
      ctcgatttt ctataatgc catatcttc atgccccac 
      ctgtcacaa atacggtta atttaggtc agaagtatc 
      gaccgactg ttattaggt ataaaagtg atgtgacaa 
      gttagtaat aatttttta ctggttcct agcagcaca 
      cataaccat tcaatatgt aatctcaag ctgaagctt 
      tcaaatgtg acaggtttt taaactaaa aattgttta 
      gaaaaatta ataaaagtt aaaaaaagt tggatggtg 
      tcaataaaa aataatgta acgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY