| General information | |||
| TC001725 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 8549703..8566521 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001725 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
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| GO ID | * {{ blurIdResult }} |
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| Evidence | {{ blurEvidenceResult }} |
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| Reference | * {{ blurReferenceResult }} |
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| Quotation | ||
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03239 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
egg chamber potentially fused - Number of animals showing the phenotype: 3
egg chamber irregular - Number of animals showing the phenotype: 3
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001725 RA
MEWTPQQEG LREILTLLK ESQSPDTAT QRTVQQKLE ELNKYPDFN NYLMFVLTK LTSEDEPTR SLSGLILKN NVKTHYNSL QPSVTNFVK NECLQAVGD PSPLIRATV GILITTIAS KGDLSSWPE LLPALCTML DSQDYNVCE GAFGALQKI CEDSAEALD ADTTNNPLE ILIPKFLQF FNHSSPKIR SYAIGCVNQ FITHRAKAL MSHIDSFLT NLFHVATDD DPEVRKNVC RALVMLLEV RLDRLIPQI ENIIEYMLV RTQDADEGV ALEACEFWL SLAEQPVCR NVLGPYLSR LIPVLVRSM KYSEIDIIL LKGDVEEDE TVPDRDEDI RPRFHKSKT TIKATSATQ NGTNSENGS VETDEDFDD GDDGDDGSL SDWNLRKCS AAALDVLAN VFREDLLPI LTPILKETL FHQDWNIKE SGILALGAI AEGCMSGMV NYLPELIPY LFSCLNDKK ALVRAITCW TLSRYSHWV VAQPHDLYL KPLMTELLK KILDGNKRV QEAACSAFA TLEEEACTE LVPYLGFIL ETLVFAFSK YQHKNLLIL YDAIGTLAD SVGHHLNKP DYISMLMPP LIQKWNILK DEDKDLFPL LECLSSIAT ALQSGFLPY CEPVYCRCV SLVQHTLYL QMANMQNPD QFDAPDKDF MIVALDLLS GLAEGLDGH IEKLVQNSN IMDLLHHCM QDSMPEVRQ SSFALLGDL TKACFQHVR PRIPDFLPI LGQNLNPEY ISVCNNATW AIGEISIKF GADTRPYIP LVLNQLIEI INRPNTPKT LSENTAITI GRLGFVCPH DVAPMLQQF VRQWCISLR NIRDNEEKD NAFRGMCHM IQLNPGGVV NDFIFFCDA VASWITPKD DLKEAFIKI LHSFKSQVG PENWRRFSD QFPPQLNER LSQLYGI
- >TC001725 RA
atggagtgg actcctcaa caggaagga ctaagggaa attttaacc ttactcaag gagtcacaa tcaccagac acagcaact caaaggact gttcagcaa aaactggag gagttgaac aagtacccc gacttcaat aattattta atgtttgtt ttgacgaaa ttaacctca gaagatgaa ccaacccgg tcgctgagt ggtttaata ttgaaaaat aacgtcaaa acacattac aacagcctc caaccaagt gtgacaaat ttcgtcaaa aatgaatgt ttacaagct gtgggtgac ccatccccc ttaatccgg gccactgtt ggtatttta ataacaaca atcgcgagc aaaggcgat ttatcgtca tggcctgaa ctattacca gctttatgc acaatgttg gactcacaa gactacaat gtttgtgaa ggggcattt ggggctttg cagaaaatt tgcgaagac tctgctgag gcgttggac gctgacacg acaaacaat cctcttgag attttaata ccgaagttt ttgcaattt ttcaaccac tcgagtccc aaaattcgg tcgtatgcc atcggatgt gtcaaccag tttatcacg catcgagct aaggctctt atgtcacac attgattca tttttaacg aatttgttc cacgttgca actgatgat gatccagaa gttcggaag aacgtttgc agggcattg gttatgctg ctagaagtg cgtctggat cggctcata cctcaaatt gaaaatata attgaatac atgttggtc agaacacaa gatgctgac gagggcgtt gcattagaa gcttgtgaa ttttggcta tctttggcc gaacaacct gtctgtaga aacgtcctt ggaccctac ctcagtaga ctgattcct gttttagtt agaagcatg aagtattca gagattgac attattttg ctcaaaggt gacgtggag gaggacgag acagttcct gatcgtgac gaggacatc cgtcctcgt ttccacaaa tcaaaaacc acaattaaa gcaacctca gcaacacag aatggcaca aattctgaa aatggtagt gtagaaacc gacgaagat tttgacgac ggagacgac ggtgatgac ggttctttg tccgattgg aatctgcga aaatgcagt gctgcagct ttggacgtg ttggcaaac gttttccgg gaagattta ttaccaatc cttacgcct atcttaaaa gagacgctt ttccaccag gactggaac atcaaagaa tctggaatt ttggcttta ggtgcgata gcagagggc tgtatgtcc ggaatggtg aattatctt cctgaattg attccgtat ctattcagt tgtttaaat gacaaaaaa gccctagtt cgagcgata acgtgttgg acacttagt agatattcg cattgggtt gtcgctcaa ccgcacgat ttatatttg aagcctctt atgactgaa ttacttaaa aaaattctt gacggtaat aaacgcgtt caagaagcg gcttgttca gcgtttgcg acattggaa gaagaagct tgcaccgaa ctcgtgcca tatttaggt tttatatta gaaacttta gtattcgcc ttttctaag taccagcac aagaatttg ttaattttg tacgacgct attggaaca ttggctgat tcagtggga caccattta aacaaaccg gattatatt agcatgctt atgccacct ttgatccag aaatggaat atcctcaaa gatgaggac aaggatctc tttccttta ttggagtgt ttatcaagt attgcaact gctcttcaa tccggattt ttgccctac tgtgaacca gtctattgc agatgtgtt tccttagtc caacacaca ctttatttg cagatggca aatatgcaa aatcctgac cagtttgat gcgcctgat aaggatttt atgattgta gctttagat ctgttgtca ggattagct gaaggctta gatggacat atagaaaaa ctagtgcaa aatagtaat atcatggac ttgcttcat cactgtatg caggattca atgcctgaa gttagacag tcatctttt gcattgctt ggtgatctg actaaagct tgttttcaa catgttcgc cctcgtata ccagatttt ttgccaata ctcgggcag aatttaaat cccgaatat atttcagtc tgcaataat gccacttgg gccattgga gaaattagt attaaattc ggtgccgat acacgacct tatatacct ttagtttta aaccaactt attgaaatt ataaacagg ccaaatact cccaaaaca ttatcagaa aatactgcc ataacaatt ggtcgacta ggttttgtc tgcccccat gatgtcgcg cccatgtta caacagttt gtacgacag tggtgcata tcattgcga aatatcagg gataatgaa gagaaagat aatgctttc cgtggcatg tgtcacatg atacagtta aatccggga ggagttgtg aacgatttc attttcttc tgtgatgct gtagcatcg tggatcaca ccaaaggat gatttgaag gaagctttt attaagatt cttcacagt tttaaatcg caagtagga ccagaaaat tggcgccga tttagcgat caatttcca ccacaactt aacgaaaga ctttcacaa ctttatgga atatga
- >TC001725 RA
ctctagtag tgaaatatt tgtgctgaa gttcgatgt cggcctcgg cctcatctt ttgtgattt tataataaa acgtgtact ttggaagca ttttattat agaattgtt gtttaaatt aaacacgac atgaaaatg gagtggact cctcaacag gaaggacta agggaaatt ttaacctta ctcaaggag tcacaatca ccagacaca gcaactcaa aggactgtt cagcaaaaa ctggaggag ttgaacaag taccccgac ttcaataat tatttaatg tttgttttg acgaaatta acctcagaa gatgaacca acccggtcg ctgagtggt ttaatattg aaaaataac gtcaaaaca cattacaac agcctccaa ccaagtgtg acaaatttc gtcaaaaat gaatgttta caagctgtg ggtgaccca tccccctta atccgggcc actgttggt attttaata acaacaatc gcgagcaaa ggcgattta tcgtcatgg cctgaacta ttaccagct ttatgcaca atgttggac tcacaagac tacaatgtt tgtgaaggg gcatttggg gctttgcag aaaatttgc gaagactct gctgaggcg ttggacgct gacacgaca aacaatcct cttgagatt ttaataccg aagtttttg caatttttc aaccactcg agtcccaaa attcggtcg tatgccatc ggatgtgtc aaccagttt atcacgcat cgagctaag gctcttatg tcacacatt gattcattt ttaacgaat ttgttccac gttgcaact gatgatgat ccagaagtt cggaagaac gtttgcagg gcattggtt atgctgcta gaagtgcgt ctggatcgg ctcatacct caaattgaa aatataatt gaatacatg ttggtcaga acacaagat gctgacgag ggcgttgca ttagaagct tgtgaattt tggctatct ttggccgaa caacctgtc tgtagaaac gtccttgga ccctacctc agtagactg attcctgtt ttagttaga agcatgaag tattcagag attgacatt attttgctc aaaggtgac gtggaggag gacgagaca gttcctgat cgtgacgag gacatccgt cctcgtttc cacaaatca aaaaccaca attaaagca acctcagca acacagaat ggcacaaat tctgaaaat ggtagtgta gaaaccgac gaagatttt gacgacgga gacgacggt gatgacggt tctttgtcc gattggaat ctgcgaaaa tgcagtgct gcagctttg gacgtgttg gcaaacgtt ttccgggaa gatttatta ccaatcctt acgcctatc ttaaaagag acgcttttc caccaggac tggaacatc aaagaatct ggaattttg gctttaggt gcgatagca gagggctgt atgtccgga atggtgaat tatcttcct gaattgatt ccgtatcta ttcagttgt ttaaatgac aaaaaagcc ctagttcga gcgataacg tgttggaca cttagtaga tattcgcat tgggttgtc gctcaaccg cacgattta tatttgaag cctcttatg actgaatta cttaaaaaa attcttgac ggtaataaa cgcgttcaa gaagcggct tgttcagcg tttgcgaca ttggaagaa gaagcttgc accgaactc gtgccatat ttaggtttt atattagaa actttagta ttcgccttt tctaagtac cagcacaag aatttgtta attttgtac gacgctatt ggaacattg gctgattca gtgggacac catttaaac aaaccggat tatattagc atgcttatg ccacctttg atccagaaa tggaatatc ctcaaagat gaggacaag gatctcttt cctttattg gagtgttta tcaagtatt gcaactgct cttcaatcc ggatttttg ccctactgt gaaccagtc tattgcaga tgtgtttcc ttagtccaa cacacactt tatttgcag atggcaaat atgcaaaat cctgaccag tttgatgcg cctgataag gattttatg attgtagct ttagatctg ttgtcagga ttagctgaa ggcttagat ggacatata gaaaaacta gtgcaaaat agtaatatc atggacttg cttcatcac tgtatgcag gattcaatg cctgaagtt agacagtca tcttttgca ttgcttggt gatctgact aaagcttgt tttcaacat gttcgccct cgtatacca gattttttg ccaatactc gggcagaat ttaaatccc gaatatatt tcagtctgc aataatgcc acttgggcc attggagaa attagtatt aaattcggt gccgataca cgaccttat ataccttta gttttaaac caacttatt gaaattata aacaggcca aatactccc aaaacatta tcagaaaat actgccata acaattggt cgactaggt tttgtctgc ccccatgat gtcgcgccc atgttacaa cagtttgta cgacagtgg tgcatatca ttgcgaaat atcagggat aatgaagag aaagataat gctttccgt ggcatgtgt cacatgata cagttaaat ccgggagga gttgtgaac gatttcatt ttcttctgt gatgctgta gcatcgtgg atcacacca aaggatgat ttgaaggaa gcttttatt aagattctt cacagtttt aaatcgcaa gtaggacca gaaaattgg cgccgattt agcgatcaa tttccacca caacttaac gaaagactt tcacaactt tatggaata tgaagtgtc acaggttag ttttgtcat tgtttaact tatcttctt cctaatcgt atttatttt ttagggtaa aaggggcta tagacaaac tgtagaatc ggggtgggg caacttgga taactcgcg ataactcag ccaggtttt accaggatc aagagggtg ggttcttta gaaacgtta cttaaatat caacaagga gaacctata ctcgcaagt tgttatttg ttaactatc acctaattt aagaagtat tgatttgtt ttcgttttt tacatggta acaaaagac tgagtatag gtttaacta attcaaatt attaatgtt aaaaaattg ggattttca atgaaaaca agtgcttaa gtgtgtagt gtttaactg ttataagtc taagtaatt ctacagtgc acttattta attaagcat cagtttaat tgttagata attcaagtt aaaagttat acacttaag ttggataaa attagacaa agtagttat gcatgtggt ttggtattt taggttgta tttgttaac aaaatgtgt gagtgctta aataactag tttcgcctt caattttgg aggggaatt aaaatcggt atttgtaac cttgatcct tagaggact tctggctcc taaaaccta gtccttccc tgctcctaa tccactgtg atttgaccg cgtgtgaat gagttttgt aatatataa agtgatgtg ataataagt tagtgaacc tgaaatcct taaagttta tttgtgata ctttttaga tataactat attaagact cgcaagtat tagtcgatg gattttttt tatagatgt tgatcaggt ttgtacagt acttgccaa aaatattgg tatcaaatt tgatttaac gacggtaag tgagatctt tcaaccttt tgtgttttt tttttgtag atgcaatat ttactcatc gttgttttg ttctaagta tgtgtgaat gagttgtcc ttttggcta cagaagtct tcgttcctt caacactcc gtgatccat tccttttgt attcctttc tcatttaag ttagattta tttaggtgt gaatatttg tttagccac cacttagcg caaaggcat atgttttct gttcatttt tttttagtt ttgtatttc atgcacagg ctaaatggg tatttatcc tcgttttca gttaacaaa tgtgattgg tgtaaaatg ttaataatt gaaattgtc actttaata aagaatata tttcaattc tttgcagtg atctctgtc ataacttca tcgaaaaaa acaattcaa acttatgtt ctttgttaa agttttcac aacaattat tttctgtaa gtacatgtg aaatgtatt attttttat aagttaata ttgtttctg aacaaataa tttcaatta aaagttcac caaagttac actcgtttt attccctgt gtctaataa ctaagtaca aataaattg aattgacaa tt
