Status
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Gene information
General information
TC001725 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 8549703..8566521
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001725
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
    {{ blurIdResult }}
    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
      {{ unavailableText }}

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03239

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 2 (9 dpi)
    Days post injection

    Fertility
    vitellogenic egg chamber late potentiallydegenerated - Number of animals showing the phenotype: 3

    egg chamber potentially fused - Number of animals showing the phenotype: 3

    egg chamber irregular - Number of animals showing the phenotype: 3

    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001725 RA
      MEWTPQQEG LREILTLLK ESQSPDTAT QRTVQQKLE 
ELNKYPDFN NYLMFVLTK LTSEDEPTR SLSGLILKN 
NVKTHYNSL QPSVTNFVK NECLQAVGD PSPLIRATV 
GILITTIAS KGDLSSWPE LLPALCTML DSQDYNVCE 
GAFGALQKI CEDSAEALD ADTTNNPLE ILIPKFLQF 
FNHSSPKIR SYAIGCVNQ FITHRAKAL MSHIDSFLT 
NLFHVATDD DPEVRKNVC RALVMLLEV RLDRLIPQI 
ENIIEYMLV RTQDADEGV ALEACEFWL SLAEQPVCR 
NVLGPYLSR LIPVLVRSM KYSEIDIIL LKGDVEEDE 
TVPDRDEDI RPRFHKSKT TIKATSATQ NGTNSENGS 
VETDEDFDD GDDGDDGSL SDWNLRKCS AAALDVLAN 
VFREDLLPI LTPILKETL FHQDWNIKE SGILALGAI 
AEGCMSGMV NYLPELIPY LFSCLNDKK ALVRAITCW 
TLSRYSHWV VAQPHDLYL KPLMTELLK KILDGNKRV 
QEAACSAFA TLEEEACTE LVPYLGFIL ETLVFAFSK 
YQHKNLLIL YDAIGTLAD SVGHHLNKP DYISMLMPP 
LIQKWNILK DEDKDLFPL LECLSSIAT ALQSGFLPY 
CEPVYCRCV SLVQHTLYL QMANMQNPD QFDAPDKDF 
MIVALDLLS GLAEGLDGH IEKLVQNSN IMDLLHHCM 
QDSMPEVRQ SSFALLGDL TKACFQHVR PRIPDFLPI 
LGQNLNPEY ISVCNNATW AIGEISIKF GADTRPYIP 
LVLNQLIEI INRPNTPKT LSENTAITI GRLGFVCPH 
DVAPMLQQF VRQWCISLR NIRDNEEKD NAFRGMCHM 
IQLNPGGVV NDFIFFCDA VASWITPKD DLKEAFIKI 
LHSFKSQVG PENWRRFSD QFPPQLNER LSQLYGI
    CDS sequence
    • >TC001725 RA
      atggagtgg actcctcaa caggaagga ctaagggaa 
attttaacc ttactcaag gagtcacaa tcaccagac 
acagcaact caaaggact gttcagcaa aaactggag 
gagttgaac aagtacccc gacttcaat aattattta 
atgtttgtt ttgacgaaa ttaacctca gaagatgaa 
ccaacccgg tcgctgagt ggtttaata ttgaaaaat 
aacgtcaaa acacattac aacagcctc caaccaagt 
gtgacaaat ttcgtcaaa aatgaatgt ttacaagct 
gtgggtgac ccatccccc ttaatccgg gccactgtt 
ggtatttta ataacaaca atcgcgagc aaaggcgat 
ttatcgtca tggcctgaa ctattacca gctttatgc 
acaatgttg gactcacaa gactacaat gtttgtgaa 
ggggcattt ggggctttg cagaaaatt tgcgaagac 
tctgctgag gcgttggac gctgacacg acaaacaat 
cctcttgag attttaata ccgaagttt ttgcaattt 
ttcaaccac tcgagtccc aaaattcgg tcgtatgcc 
atcggatgt gtcaaccag tttatcacg catcgagct 
aaggctctt atgtcacac attgattca tttttaacg 
aatttgttc cacgttgca actgatgat gatccagaa 
gttcggaag aacgtttgc agggcattg gttatgctg 
ctagaagtg cgtctggat cggctcata cctcaaatt 
gaaaatata attgaatac atgttggtc agaacacaa 
gatgctgac gagggcgtt gcattagaa gcttgtgaa 
ttttggcta tctttggcc gaacaacct gtctgtaga 
aacgtcctt ggaccctac ctcagtaga ctgattcct 
gttttagtt agaagcatg aagtattca gagattgac 
attattttg ctcaaaggt gacgtggag gaggacgag 
acagttcct gatcgtgac gaggacatc cgtcctcgt 
ttccacaaa tcaaaaacc acaattaaa gcaacctca 
gcaacacag aatggcaca aattctgaa aatggtagt 
gtagaaacc gacgaagat tttgacgac ggagacgac 
ggtgatgac ggttctttg tccgattgg aatctgcga 
aaatgcagt gctgcagct ttggacgtg ttggcaaac 
gttttccgg gaagattta ttaccaatc cttacgcct 
atcttaaaa gagacgctt ttccaccag gactggaac 
atcaaagaa tctggaatt ttggcttta ggtgcgata 
gcagagggc tgtatgtcc ggaatggtg aattatctt 
cctgaattg attccgtat ctattcagt tgtttaaat 
gacaaaaaa gccctagtt cgagcgata acgtgttgg 
acacttagt agatattcg cattgggtt gtcgctcaa 
ccgcacgat ttatatttg aagcctctt atgactgaa 
ttacttaaa aaaattctt gacggtaat aaacgcgtt 
caagaagcg gcttgttca gcgtttgcg acattggaa 
gaagaagct tgcaccgaa ctcgtgcca tatttaggt 
tttatatta gaaacttta gtattcgcc ttttctaag 
taccagcac aagaatttg ttaattttg tacgacgct 
attggaaca ttggctgat tcagtggga caccattta 
aacaaaccg gattatatt agcatgctt atgccacct 
ttgatccag aaatggaat atcctcaaa gatgaggac 
aaggatctc tttccttta ttggagtgt ttatcaagt 
attgcaact gctcttcaa tccggattt ttgccctac 
tgtgaacca gtctattgc agatgtgtt tccttagtc 
caacacaca ctttatttg cagatggca aatatgcaa 
aatcctgac cagtttgat gcgcctgat aaggatttt 
atgattgta gctttagat ctgttgtca ggattagct 
gaaggctta gatggacat atagaaaaa ctagtgcaa 
aatagtaat atcatggac ttgcttcat cactgtatg 
caggattca atgcctgaa gttagacag tcatctttt 
gcattgctt ggtgatctg actaaagct tgttttcaa 
catgttcgc cctcgtata ccagatttt ttgccaata 
ctcgggcag aatttaaat cccgaatat atttcagtc 
tgcaataat gccacttgg gccattgga gaaattagt 
attaaattc ggtgccgat acacgacct tatatacct 
ttagtttta aaccaactt attgaaatt ataaacagg 
ccaaatact cccaaaaca ttatcagaa aatactgcc 
ataacaatt ggtcgacta ggttttgtc tgcccccat 
gatgtcgcg cccatgtta caacagttt gtacgacag 
tggtgcata tcattgcga aatatcagg gataatgaa 
gagaaagat aatgctttc cgtggcatg tgtcacatg 
atacagtta aatccggga ggagttgtg aacgatttc 
attttcttc tgtgatgct gtagcatcg tggatcaca 
ccaaaggat gatttgaag gaagctttt attaagatt 
cttcacagt tttaaatcg caagtagga ccagaaaat 
tggcgccga tttagcgat caatttcca ccacaactt 
aacgaaaga ctttcacaa ctttatgga atatga
    mRNA sequence
    • >TC001725 RA
      ctctagtag tgaaatatt tgtgctgaa gttcgatgt 
cggcctcgg cctcatctt ttgtgattt tataataaa 
acgtgtact ttggaagca ttttattat agaattgtt 
gtttaaatt aaacacgac atgaaaatg gagtggact 
cctcaacag gaaggacta agggaaatt ttaacctta 
ctcaaggag tcacaatca ccagacaca gcaactcaa 
aggactgtt cagcaaaaa ctggaggag ttgaacaag 
taccccgac ttcaataat tatttaatg tttgttttg 
acgaaatta acctcagaa gatgaacca acccggtcg 
ctgagtggt ttaatattg aaaaataac gtcaaaaca 
cattacaac agcctccaa ccaagtgtg acaaatttc 
gtcaaaaat gaatgttta caagctgtg ggtgaccca 
tccccctta atccgggcc actgttggt attttaata 
acaacaatc gcgagcaaa ggcgattta tcgtcatgg 
cctgaacta ttaccagct ttatgcaca atgttggac 
tcacaagac tacaatgtt tgtgaaggg gcatttggg 
gctttgcag aaaatttgc gaagactct gctgaggcg 
ttggacgct gacacgaca aacaatcct cttgagatt 
ttaataccg aagtttttg caatttttc aaccactcg 
agtcccaaa attcggtcg tatgccatc ggatgtgtc 
aaccagttt atcacgcat cgagctaag gctcttatg 
tcacacatt gattcattt ttaacgaat ttgttccac 
gttgcaact gatgatgat ccagaagtt cggaagaac 
gtttgcagg gcattggtt atgctgcta gaagtgcgt 
ctggatcgg ctcatacct caaattgaa aatataatt 
gaatacatg ttggtcaga acacaagat gctgacgag 
ggcgttgca ttagaagct tgtgaattt tggctatct 
ttggccgaa caacctgtc tgtagaaac gtccttgga 
ccctacctc agtagactg attcctgtt ttagttaga 
agcatgaag tattcagag attgacatt attttgctc 
aaaggtgac gtggaggag gacgagaca gttcctgat 
cgtgacgag gacatccgt cctcgtttc cacaaatca 
aaaaccaca attaaagca acctcagca acacagaat 
ggcacaaat tctgaaaat ggtagtgta gaaaccgac 
gaagatttt gacgacgga gacgacggt gatgacggt 
tctttgtcc gattggaat ctgcgaaaa tgcagtgct 
gcagctttg gacgtgttg gcaaacgtt ttccgggaa 
gatttatta ccaatcctt acgcctatc ttaaaagag 
acgcttttc caccaggac tggaacatc aaagaatct 
ggaattttg gctttaggt gcgatagca gagggctgt 
atgtccgga atggtgaat tatcttcct gaattgatt 
ccgtatcta ttcagttgt ttaaatgac aaaaaagcc 
ctagttcga gcgataacg tgttggaca cttagtaga 
tattcgcat tgggttgtc gctcaaccg cacgattta 
tatttgaag cctcttatg actgaatta cttaaaaaa 
attcttgac ggtaataaa cgcgttcaa gaagcggct 
tgttcagcg tttgcgaca ttggaagaa gaagcttgc 
accgaactc gtgccatat ttaggtttt atattagaa 
actttagta ttcgccttt tctaagtac cagcacaag 
aatttgtta attttgtac gacgctatt ggaacattg 
gctgattca gtgggacac catttaaac aaaccggat 
tatattagc atgcttatg ccacctttg atccagaaa 
tggaatatc ctcaaagat gaggacaag gatctcttt 
cctttattg gagtgttta tcaagtatt gcaactgct 
cttcaatcc ggatttttg ccctactgt gaaccagtc 
tattgcaga tgtgtttcc ttagtccaa cacacactt 
tatttgcag atggcaaat atgcaaaat cctgaccag 
tttgatgcg cctgataag gattttatg attgtagct 
ttagatctg ttgtcagga ttagctgaa ggcttagat 
ggacatata gaaaaacta gtgcaaaat agtaatatc 
atggacttg cttcatcac tgtatgcag gattcaatg 
cctgaagtt agacagtca tcttttgca ttgcttggt 
gatctgact aaagcttgt tttcaacat gttcgccct 
cgtatacca gattttttg ccaatactc gggcagaat 
ttaaatccc gaatatatt tcagtctgc aataatgcc 
acttgggcc attggagaa attagtatt aaattcggt 
gccgataca cgaccttat ataccttta gttttaaac 
caacttatt gaaattata aacaggcca aatactccc 
aaaacatta tcagaaaat actgccata acaattggt 
cgactaggt tttgtctgc ccccatgat gtcgcgccc 
atgttacaa cagtttgta cgacagtgg tgcatatca 
ttgcgaaat atcagggat aatgaagag aaagataat 
gctttccgt ggcatgtgt cacatgata cagttaaat 
ccgggagga gttgtgaac gatttcatt ttcttctgt 
gatgctgta gcatcgtgg atcacacca aaggatgat 
ttgaaggaa gcttttatt aagattctt cacagtttt 
aaatcgcaa gtaggacca gaaaattgg cgccgattt 
agcgatcaa tttccacca caacttaac gaaagactt 
tcacaactt tatggaata tgaagtgtc acaggttag 
ttttgtcat tgtttaact tatcttctt cctaatcgt 
atttatttt ttagggtaa aaggggcta tagacaaac 
tgtagaatc ggggtgggg caacttgga taactcgcg 
ataactcag ccaggtttt accaggatc aagagggtg 
ggttcttta gaaacgtta cttaaatat caacaagga 
gaacctata ctcgcaagt tgttatttg ttaactatc 
acctaattt aagaagtat tgatttgtt ttcgttttt 
tacatggta acaaaagac tgagtatag gtttaacta 
attcaaatt attaatgtt aaaaaattg ggattttca 
atgaaaaca agtgcttaa gtgtgtagt gtttaactg 
ttataagtc taagtaatt ctacagtgc acttattta 
attaagcat cagtttaat tgttagata attcaagtt 
aaaagttat acacttaag ttggataaa attagacaa 
agtagttat gcatgtggt ttggtattt taggttgta 
tttgttaac aaaatgtgt gagtgctta aataactag 
tttcgcctt caattttgg aggggaatt aaaatcggt 
atttgtaac cttgatcct tagaggact tctggctcc 
taaaaccta gtccttccc tgctcctaa tccactgtg 
atttgaccg cgtgtgaat gagttttgt aatatataa 
agtgatgtg ataataagt tagtgaacc tgaaatcct 
taaagttta tttgtgata ctttttaga tataactat 
attaagact cgcaagtat tagtcgatg gattttttt 
tatagatgt tgatcaggt ttgtacagt acttgccaa 
aaatattgg tatcaaatt tgatttaac gacggtaag 
tgagatctt tcaaccttt tgtgttttt tttttgtag 
atgcaatat ttactcatc gttgttttg ttctaagta 
tgtgtgaat gagttgtcc ttttggcta cagaagtct 
tcgttcctt caacactcc gtgatccat tccttttgt 
attcctttc tcatttaag ttagattta tttaggtgt 
gaatatttg tttagccac cacttagcg caaaggcat 
atgttttct gttcatttt tttttagtt ttgtatttc 
atgcacagg ctaaatggg tatttatcc tcgttttca 
gttaacaaa tgtgattgg tgtaaaatg ttaataatt 
gaaattgtc actttaata aagaatata tttcaattc 
tttgcagtg atctctgtc ataacttca tcgaaaaaa 
acaattcaa acttatgtt ctttgttaa agttttcac 
aacaattat tttctgtaa gtacatgtg aaatgtatt 
attttttat aagttaata ttgtttctg aacaaataa 
tttcaatta aaagttcac caaagttac actcgtttt 
attccctgt gtctaataa ctaagtaca aataaattg 
aattgacaa tt

    If you use iBeetle-Base, please cite: Dönitz J et al.: Expanded and updated data and a query pipeline for iBeetle-Base,
    Nucleic Acids Res. 2018 Jan 4;46(D1):D831-D835. doi: 10.1093/nar/gkx984.

    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY