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Gene information
General information
TC001694 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7718938..7722234
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      		TC001694
      iBeetle screen iB_06631

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection

      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
       embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      iBeetle screen iB_03234

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
       head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      head appendages random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      abdomen partially constricted - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      abdominal segment 1 constricted - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Comment: Constriction often affecting A1 or T3/-A2
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001694 RA
        MSNESAELT KSWCLVDSS GVQEVTENE THDSDTDSI 
SVISESGCS YRTDEDEVA TPFEEPQLS EEAPVGEPE 
IVEEPPPLD EVKSIEKFL YLATSAFCA VIIIGFSVA 
VQQYLYNSS DVDNYEPDF AFPRVQNIK TDPPKKYPP 
KRKSHIKLD ELENKFAKL EVDNERHDK IAPAGGDND 
PRAEGNLKS NYKLNKNKS PVKCNKENC DAVRNDQEN 
RNDETYDKK IKELKVKED YLREKEKFL IEKEHDLLK 
KEIKLNREK ESYDNTKND KKRHYKEDD AKKPKDRKK 
KQKLAEEPY RKRNLSGQW YISMHEARD NMRQKKRSL 
FKKNKAHWY FQWMVDRAR LRVK
      CDS sequence
      • >TC001694 RA
        atgagcaac gagagtgcc gaactgacg aagtcatgg 
tgccttgtg gactcctca ggagtgcag gaagttacc 
gagaatgaa acgcacgat tccgacaca gacagtatt 
agcgttatc agtgaaagt ggctgttcg tacaggaca 
gacgaggat gaagtagca acccctttt gaagaaccc 
caattaagc gaggaagct ccagttggt gagcctgaa 
attgtggaa gaaccaccg ccgctagac gaggtcaaa 
agcatcgaa aaattcctc tatctcgcc acgagcgcc 
ttttgcgct gttataata atcgggttc tcggttgcg 
gtgcaacaa tacctttac aattcttct gatgtagat 
aattatgaa ccggatttc gcgttccct cgtgtacaa 
aatatcaaa acagaccct ccaaaaaag taccccccg 
aagcggaaa agtcacatc aaactagac gaattagaa 
aataagttt gctaaatta gaagtagac aacgaaagg 
catgacaag atcgcaccg gctggtgga gacaatgac 
ccccgagcc gagggaaat ctcaaatct aattataaa 
cttaacaaa aataaaagc ccagtcaaa tgtaacaaa 
gaaaattgc gacgcagta cgaaacgac caagaaaat 
cgaaatgac gagacctac gacaaaaaa atcaaggaa 
ctgaaagtg aaggaggat tatctcaga gagaaggaa 
aagtttctc attgagaaa gaacacgac ttgttgaag 
aaggagatc aagcttaac agggagaag gagagctac 
gacaatacc aaaaatgac aagaaaaga cattataag 
gaggatgat gctaaaaag cccaaagac cgcaagaag 
aaacaaaaa ctggccgag gagccgtat agaaaacgc 
aacctttcg ggccagtgg tatataagc atgcacgaa 
gctagggac aacatgcgg cagaaaaaa cggtcgctg 
tttaaaaaa aacaaggct cattggtac ttccagtgg 
atggtggat agagcaagg cttcgcgtc aagtga
      mRNA sequence
      • >TC001694 RA
        agctgacaa tgacaggga atgaaaata aacaaatag 
taaaaacgc aatttcatt gttgatgtt cccagaagt 
gcagaatga actgatttg cacgtaaaa catcacgga 
cgggtgcag agttgcgag ttggccacc ttacatgag 
tcagggaag tgcagcacg cggcatttt gcacaaaga 
aagctgaca tattgttgc gtcgttaga caatgagca 
acgagagtg ccgaactga cgaagtcat ggtgccttg 
tggactcct caggagtgc aggaagtta ccgagaatg 
aaacgcacg attccgaca cagacagta ttagcgtta 
tcagtgaaa gtggctgtt cgtacagga cagacgagg 
atgaagtag caacccctt ttgaagaac cccaattaa 
gcgaggaag ctccagttg gtgagcctg aaattgtgg 
aagaaccac cgccgctag acgaggtca aaagcatcg 
aaaaattcc tctatctcg ccacgagcg ccttttgcg 
ctgttataa taatcgggt tctcggttg cggtgcaac 
aataccttt acaattctt ctgatgtag ataattatg 
aaccggatt tcgcgttcc ctcgtgtac aaaatatca 
aaacagacc ctccaaaaa agtaccccc cgaagcgga 
aaagtcaca tcaaactag acgaattag aaaataagt 
ttgctaaat tagaagtag acaacgaaa ggcatgaca 
agatcgcac cggctggtg gagacaatg acccccgag 
ccgagggaa atctcaaat ctaattata aacttaaca 
aaaataaaa gcccagtca aatgtaaca aagaaaatt 
gcgacgcag tacgaaacg accaagaaa atcgaaatg 
acgagacct acgacaaaa aaatcaagg aactgaaag 
tgaaggagg attatctca gagagaagg aaaagtttc 
tcattgaga aagaacacg acttgttga agaaggaga 
tcaagctta acagggaga aggagagct acgacaata 
ccaaaaatg acaagaaaa gacattata aggaggatg 
atgctaaaa agcccaaag accgcaaga agaaacaaa 
aactggccg aggagccgt atagaaaac gcaaccttt 
cgggccagt ggtatataa gcatgcacg aagctaggg 
acaacatgc ggcagaaaa aacggtcgc tgtttaaaa 
aaaacaagg ctcattggt acttccagt ggatggtgg 
atagagcaa ggcttcgcg tcaagtgaa ctatttttc 
taaattgtc actctgcat tttatttga catttcgtt 
tgagtatta ttgttttta tgtactttt tatttcgta 
attgtgaat tttttcgat tattttaat aaagactca 
cttatttgg ggttgtttt aatcgagtg ggagctggg 
cgggtaagg cgaataaaa atcacttgt atgaaataa 
accctctga tttattctt aaaattaag gtcatacaa 
tcgcttctt gttaagcat actacttaa taatagtaa 
taataatag taataacca ctactgacg tttacacgt 
ccaacaatt gaatcaaaa atatcaatg gcactggtt 
aaatatcac ccgattcta taagtatat gcgtaagta 
acaaatact tttgttatt tatttacgg tataattgt 
aacattgga gtacatgag taaattttc caaattatt 
agatcttca cgtgtttga ctaacagct tcctaacag 
tattattag tgattaaaa gcttgaatt agttaaaaa 
atatgagta cagacctgg ttaagtatt aatgtttaa 
agcttgaaa acaattgta ctacaatta cgtattaag 
taaagtaaa tgtaaatat tagacggta taatctcgt 
ttccgtttt aaatttagt ataaaaaat ctacacctc 
taacttcat acaccgagt tagttattg acttggatg 
ttacgatgg atggatacg cgatggatt cacctagtg 
tttacatta tatcacatt gttatattt cttaaccaa 
taagctttt gaatttcgt cgatgaagg tctaaaaat 
ttgtactaa ggaaaaggg aagtaaacg aaatgcaga 
gtgcccaga agagcaata cttgttaat cagagcgga 
gggataggt acatcagtc ggggtcact ctgctatac 
gcgactaga aatgagaag ataaaggag tcgggatga 
gcatgacgt gggggacgc ccgggactg tccgtcgca 
ccctcgctc ggccgcggc tgctttaaa ggtactcaa 
tcacagtag atttaacaa ccgacacgg ccacaccgc 
cggagcccc caaaaaacc ctctcatct accaagcac 
caagcacca aacccgggg gctccgacg ctgcgtggt 
gccgcaata tacaatagt atataaata tatgttata 
tacatgtga actataatg ccatttggt gcagctggc 
aactcacgt atagtcgta atatacagt tgagctggc 
gttgcgctg tgagcaagc tcggttata acgtctcta 
atataacta agctacggt aacagtaat attattgta 
atactaagg cacagaccc agctcatta cttgttcgc 
tcaacaagc acgtttgtg gaagacaag catcgctgg 
atattctac ccaactctg gggacgccc acccccgca 
ccgccatct cccaccaaa caatcggca taaaccaaa 
gtagcgttg gattcgtcc cgggcaacg caactatca 
ttagaaatc cacagaaat ccctgtaat ccgcataaa 
tcccaagta ctactctca agtcgctca ctctttttc 
gacactgtc ctgttttat atccataca tagaacatc 
taacgaacc aaactgaat ggtacaatt ttcggataa 
aaccaagaa acaagactt taccgagca gactactaa 
cacctattt tctatttat aatttccga tgaaagaat 
tcgttttcg ctatgaaaa ttgccagtg aaaaaattt 
tcaacaatt tttctggtt cgttataag cagacattg 
ctgcacaag taatatctc tatcttgta aatttggcg 
ctcaaattg aaaagtttt ttcatcgat ttttataaa 
atgcgaaga acatacaaa tcatacaaa aaaactttg 
aagtagagt ccgacgtat ctaagaaaa tcgcgattt 
ccaattgtt tcattattt ggacctaag tatttgatt 
taaagtcat agtctgacc tattgaaaa taaaatata 
acccaagtt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY