Status
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Gene information
General information
TC001694 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7718938..7722234
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC001694
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
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        Status: {{ goTerm.status }}
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        Quotation: {{ goTerm.quotation }}
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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_06631

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

      adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
      Days post injection


      Analysis of embryonic musculature and early development
      number of eggs/embryos/larvae on slide: > 50 embryo/egg not developed/not fertilized - Penetrance (percentage of animals/eggs on slide): more than 80%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: > 50
      embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): more than 80%
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      iBeetle screen iB_03234

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of embryonic musculature and early development
      larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): 30% - 50%

      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 30% - 50%
      remarks: muscle pattern potentially obscured by segmentation defect,
      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 50
      head appendages position irregular - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      head appendages random not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      head capsule mostly not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      abdomen partially constricted - Penetrance (percentage of animals/eggs on slide): 50% - 80%

      abdominal segment 1 constricted - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Comment: Constriction often affecting A1 or T3/-A2
      affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC001694 RA
        MSNESAELT KSWCLVDSS GVQEVTENE THDSDTDSI 
        SVISESGCS YRTDEDEVA TPFEEPQLS EEAPVGEPE 
        IVEEPPPLD EVKSIEKFL YLATSAFCA VIIIGFSVA 
        VQQYLYNSS DVDNYEPDF AFPRVQNIK TDPPKKYPP 
        KRKSHIKLD ELENKFAKL EVDNERHDK IAPAGGDND 
        PRAEGNLKS NYKLNKNKS PVKCNKENC DAVRNDQEN 
        RNDETYDKK IKELKVKED YLREKEKFL IEKEHDLLK 
        KEIKLNREK ESYDNTKND KKRHYKEDD AKKPKDRKK 
        KQKLAEEPY RKRNLSGQW YISMHEARD NMRQKKRSL 
        FKKNKAHWY FQWMVDRAR LRVK
      CDS sequence
      • >TC001694 RA
        atgagcaac gagagtgcc gaactgacg aagtcatgg 
        tgccttgtg gactcctca ggagtgcag gaagttacc 
        gagaatgaa acgcacgat tccgacaca gacagtatt 
        agcgttatc agtgaaagt ggctgttcg tacaggaca 
        gacgaggat gaagtagca acccctttt gaagaaccc 
        caattaagc gaggaagct ccagttggt gagcctgaa 
        attgtggaa gaaccaccg ccgctagac gaggtcaaa 
        agcatcgaa aaattcctc tatctcgcc acgagcgcc 
        ttttgcgct gttataata atcgggttc tcggttgcg 
        gtgcaacaa tacctttac aattcttct gatgtagat 
        aattatgaa ccggatttc gcgttccct cgtgtacaa 
        aatatcaaa acagaccct ccaaaaaag taccccccg 
        aagcggaaa agtcacatc aaactagac gaattagaa 
        aataagttt gctaaatta gaagtagac aacgaaagg 
        catgacaag atcgcaccg gctggtgga gacaatgac 
        ccccgagcc gagggaaat ctcaaatct aattataaa 
        cttaacaaa aataaaagc ccagtcaaa tgtaacaaa 
        gaaaattgc gacgcagta cgaaacgac caagaaaat 
        cgaaatgac gagacctac gacaaaaaa atcaaggaa 
        ctgaaagtg aaggaggat tatctcaga gagaaggaa 
        aagtttctc attgagaaa gaacacgac ttgttgaag 
        aaggagatc aagcttaac agggagaag gagagctac 
        gacaatacc aaaaatgac aagaaaaga cattataag 
        gaggatgat gctaaaaag cccaaagac cgcaagaag 
        aaacaaaaa ctggccgag gagccgtat agaaaacgc 
        aacctttcg ggccagtgg tatataagc atgcacgaa 
        gctagggac aacatgcgg cagaaaaaa cggtcgctg 
        tttaaaaaa aacaaggct cattggtac ttccagtgg 
        atggtggat agagcaagg cttcgcgtc aagtga
      mRNA sequence
      • >TC001694 RA
        agctgacaa tgacaggga atgaaaata aacaaatag 
        taaaaacgc aatttcatt gttgatgtt cccagaagt 
        gcagaatga actgatttg cacgtaaaa catcacgga 
        cgggtgcag agttgcgag ttggccacc ttacatgag 
        tcagggaag tgcagcacg cggcatttt gcacaaaga 
        aagctgaca tattgttgc gtcgttaga caatgagca 
        acgagagtg ccgaactga cgaagtcat ggtgccttg 
        tggactcct caggagtgc aggaagtta ccgagaatg 
        aaacgcacg attccgaca cagacagta ttagcgtta 
        tcagtgaaa gtggctgtt cgtacagga cagacgagg 
        atgaagtag caacccctt ttgaagaac cccaattaa 
        gcgaggaag ctccagttg gtgagcctg aaattgtgg 
        aagaaccac cgccgctag acgaggtca aaagcatcg 
        aaaaattcc tctatctcg ccacgagcg ccttttgcg 
        ctgttataa taatcgggt tctcggttg cggtgcaac 
        aataccttt acaattctt ctgatgtag ataattatg 
        aaccggatt tcgcgttcc ctcgtgtac aaaatatca 
        aaacagacc ctccaaaaa agtaccccc cgaagcgga 
        aaagtcaca tcaaactag acgaattag aaaataagt 
        ttgctaaat tagaagtag acaacgaaa ggcatgaca 
        agatcgcac cggctggtg gagacaatg acccccgag 
        ccgagggaa atctcaaat ctaattata aacttaaca 
        aaaataaaa gcccagtca aatgtaaca aagaaaatt 
        gcgacgcag tacgaaacg accaagaaa atcgaaatg 
        acgagacct acgacaaaa aaatcaagg aactgaaag 
        tgaaggagg attatctca gagagaagg aaaagtttc 
        tcattgaga aagaacacg acttgttga agaaggaga 
        tcaagctta acagggaga aggagagct acgacaata 
        ccaaaaatg acaagaaaa gacattata aggaggatg 
        atgctaaaa agcccaaag accgcaaga agaaacaaa 
        aactggccg aggagccgt atagaaaac gcaaccttt 
        cgggccagt ggtatataa gcatgcacg aagctaggg 
        acaacatgc ggcagaaaa aacggtcgc tgtttaaaa 
        aaaacaagg ctcattggt acttccagt ggatggtgg 
        atagagcaa ggcttcgcg tcaagtgaa ctatttttc 
        taaattgtc actctgcat tttatttga catttcgtt 
        tgagtatta ttgttttta tgtactttt tatttcgta 
        attgtgaat tttttcgat tattttaat aaagactca 
        cttatttgg ggttgtttt aatcgagtg ggagctggg 
        cgggtaagg cgaataaaa atcacttgt atgaaataa 
        accctctga tttattctt aaaattaag gtcatacaa 
        tcgcttctt gttaagcat actacttaa taatagtaa 
        taataatag taataacca ctactgacg tttacacgt 
        ccaacaatt gaatcaaaa atatcaatg gcactggtt 
        aaatatcac ccgattcta taagtatat gcgtaagta 
        acaaatact tttgttatt tatttacgg tataattgt 
        aacattgga gtacatgag taaattttc caaattatt 
        agatcttca cgtgtttga ctaacagct tcctaacag 
        tattattag tgattaaaa gcttgaatt agttaaaaa 
        atatgagta cagacctgg ttaagtatt aatgtttaa 
        agcttgaaa acaattgta ctacaatta cgtattaag 
        taaagtaaa tgtaaatat tagacggta taatctcgt 
        ttccgtttt aaatttagt ataaaaaat ctacacctc 
        taacttcat acaccgagt tagttattg acttggatg 
        ttacgatgg atggatacg cgatggatt cacctagtg 
        tttacatta tatcacatt gttatattt cttaaccaa 
        taagctttt gaatttcgt cgatgaagg tctaaaaat 
        ttgtactaa ggaaaaggg aagtaaacg aaatgcaga 
        gtgcccaga agagcaata cttgttaat cagagcgga 
        gggataggt acatcagtc ggggtcact ctgctatac 
        gcgactaga aatgagaag ataaaggag tcgggatga 
        gcatgacgt gggggacgc ccgggactg tccgtcgca 
        ccctcgctc ggccgcggc tgctttaaa ggtactcaa 
        tcacagtag atttaacaa ccgacacgg ccacaccgc 
        cggagcccc caaaaaacc ctctcatct accaagcac 
        caagcacca aacccgggg gctccgacg ctgcgtggt 
        gccgcaata tacaatagt atataaata tatgttata 
        tacatgtga actataatg ccatttggt gcagctggc 
        aactcacgt atagtcgta atatacagt tgagctggc 
        gttgcgctg tgagcaagc tcggttata acgtctcta 
        atataacta agctacggt aacagtaat attattgta 
        atactaagg cacagaccc agctcatta cttgttcgc 
        tcaacaagc acgtttgtg gaagacaag catcgctgg 
        atattctac ccaactctg gggacgccc acccccgca 
        ccgccatct cccaccaaa caatcggca taaaccaaa 
        gtagcgttg gattcgtcc cgggcaacg caactatca 
        ttagaaatc cacagaaat ccctgtaat ccgcataaa 
        tcccaagta ctactctca agtcgctca ctctttttc 
        gacactgtc ctgttttat atccataca tagaacatc 
        taacgaacc aaactgaat ggtacaatt ttcggataa 
        aaccaagaa acaagactt taccgagca gactactaa 
        cacctattt tctatttat aatttccga tgaaagaat 
        tcgttttcg ctatgaaaa ttgccagtg aaaaaattt 
        tcaacaatt tttctggtt cgttataag cagacattg 
        ctgcacaag taatatctc tatcttgta aatttggcg 
        ctcaaattg aaaagtttt ttcatcgat ttttataaa 
        atgcgaaga acatacaaa tcatacaaa aaaactttg 
        aagtagagt ccgacgtat ctaagaaaa tcgcgattt 
        ccaattgtt tcattattt ggacctaag tatttgatt 
        taaagtcat agtctgacc tattgaaaa taaaatata 
        acccaagtt
      The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY