Status
Please wait ...
Gene information
General information
TC001693 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 7689825..7699959
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001693
    iBeetle screen iB_03233

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 50
    head appendages mostly shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%

    maxilla distal structure not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    labium distal structure not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    head appendages mostly size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%

    vertex triplet vertex triplet ventral not present - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    legs podomer mostly not present - Penetrance (percentage of animals/eggs on slide): more than 80%

    Comment: Coxa, tibiotarsus (potentially) and pretarsus present.
    legs size decreased - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs shape irregular - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs trochanter potentially not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    legs femur not present - Penetrance (percentage of animals/eggs on slide): more than 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): more than 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001693 RA
      MTGGAIHVP LCPFKGYRH LHRASTLAP PQRSRLLPV 
      CARKLIPTF VTALDKLGT RHLFSNSAP EPVQVYAAF 
      DIASLVLYG CQALPIRLK ILLDRLFSV LRKEQVLQV 
      LHGFGWTAE DYTRGYILQ ESHGGTLDR WSICSPEEE 
      PLVLQQFLR FGETRAITQ QLLLAQQAL QDPTLERLE 
      RRHSPNLRR AMSPNHRPS HQTVPSAFH PSRLPTMSP 
      HQKHHLNFL KMSTSNGPL PSNSPPRSV STSPLNTSP 
      LNRLQNMQP FDFRKLGSG LTNFPNRVS PDMNRRRAE 
      SESVGLNLT MATSLSSCL PPPPPRPPS LSHSAAAMA 
      AAVSVNPLA AASLVASSF SGLMSSRTP RSKSPSGEV 
      LSGNNNSEE DDDVDENSY SALNLSRDR DILKAPRQP 
      RGIPGRKPT TPTKRQWGS PGLPLNLGT QFINPATGK 
      KRVQCNVCL KTFCDKGAL KIHFSAVHL REMHKCTVE 
      GCNMMFSSR RSRNRHSAN PNPKLHSPH LRRKISPHD 
      GRSAQAHPL LIPPQTGLS LPAAAAALN PLNPFGPFP 
      LLTPSPDIR HHTALSSID FKQTLDLSM QKLEERKHS 
      SVKDYSALS LVGGTHHQS EEEEDEDDD GIVVVGGDE 
      EEEPEKSEV TNGVSDQPE DFSMPSKRP KMSVSDVEE 
      DITSNLDSN EDSLSIVDT HSLKDELNV QTNKRKRKS 
      QNPTRCAVP AMMEDAVSD CDSSNDVFS ESLNEKTHT 
      NDEKRSPSR LEAKKEEDK FEEKCDSPL NLEKRCPDV 
      NANEPEKIA SPEIKKSLA PLFMIDKLK KEKPSHDSE 
      PVEETDERP ASSVESNKS DESFDSSNA LRHLESLSH 
      GHFGDLMAK GLHLGLGQG PQFPPIAFM MGGGPPSPA 
      RSQASSAGS SNGAESPDE NSQNQFFGH FDNGQFISG 
      MDVPIDKDN PRKCTACGK IFQNHFGVK THYQNVHLK 
      LMHKCNVDG CNAAFPSKR SRDRHSANL NLHRKLLST 
      SSDKSAASL FLEKSPFAL PGDFLTRLY AEEALKNHH 
      LPPPNLDQL MLNGDRIPH PPLLLPPLG GIPFPLGSF 
      NHFAQFNGS STMKERSSR SSSPVSGSP PPTNPSPVS 
      LKYTHCVDE DQPTPDKDG NLPCRLCKT PFPTSSQLK 
      EHCESNHLS EMFKCTVMG CNKVFMSRT RRNLHAENE 
      SLHVSSSRK SSESS
    CDS sequence
    • >TC001693 RA
      atgacgggg ggtgcaata catgtccca ctatgcccg 
      ttcaagggg tatcgtcac ctacaccgt gcgtccacc 
      ttggcaccc ccccaaagg agtcggctc ctccccgtt 
      tgcgcacgc aaacttatc cccactttt gttacagca 
      ctggacaag ttgggaacg cgacatctc ttcagtaac 
      agtgccccc gaaccagtg caagtttac gccgcgttt 
      gacatcgct agtttagtc ctttacgga tgtcaagcc 
      ctcccaatt cgccttaaa atactcctg gatagactg 
      ttttcggtg cttcgcaaa gagcaagtg cttcaagtg 
      ttgcatgga tttggctgg accgccgaa gattacaca 
      agaggatat atactgcag gagtcacac ggcggcact 
      ctggaccga tggagcatc tgcagtcct gaggaggag 
      cctctggtc ctccaacag ttccttagg tttggagag 
      acgcgagcc ataacgcaa caattgctt ctggcgcag 
      caggcactt caggatccc acactggag cgcctagaa 
      cgccgccac tcgccaaac cttcgtcgg gcgatgtca 
      cctaaccat cgcccgtcc caccaaact gttccatct 
      gcttttcac ccgtctcgc ttaccgaca atgtctcct 
      catcaaaaa catcactta aatttcttg aagatgtct 
      acctccaac ggacctctc ccctccaac tctcctccg 
      agaagcgtc agtactagt ccattgaac acgtcaccc 
      ttgaaccgt ctacaaaac atgcaacct ttcgatttc 
      cgtaaattg ggtagcggg ctgacgaat tttccgaac 
      cgtgtaagt cccgacatg aatcgaaga agggcggaa 
      agtgagagc gtgggcttg aatttgacg atggccacg 
      tcgttgtct tcgtgtttg ccgccgccg cctccacgg 
      cccccgtct ttgagccac tcggcggcg gcaatggca 
      gccgctgtt tcggtcaat cccttggcg gcggccagt 
      ttggtggcc tcgtccttc tcgggactt atgtcatcg 
      aggacaccg aggagcaaa tcaccaagt ggggaagtt 
      ttaagtggc aataataat agtgaagaa gacgatgat 
      gttgacgaa aactcttat agtgcactt aatttaagt 
      cgtgataga gatattctc aaagcccca agacaaccg 
      aggggaatc ccgggacga aaacccaca acacctact 
      aaaagacag tggggatcg ccgggattg cctctgaat 
      ctaggaacg caattcatc aacccggcc acagggaag 
      aaaagagtg caatgtaac gtatgttta aagacgttt 
      tgcgacaaa ggtgctctt aaaattcat ttctcggcg 
      gtgcattta cgtgaaatg cacaaatgt acggttgaa 
      ggttgtaat atgatgttt agttcaaga aggtcgaga 
      aatcgtcat agtgctaat ccaaatcca aaattgcat 
      tcacctcat cttcgtaga aaaatttcg ccgcacgat 
      ggaagaagt gcacaagct catcctctc ctaatacct 
      ccccaaact ggtctttca ctaccagca gcagctgct 
      gccttgaat ccgctcaat cctttcgga ccatttccc 
      ttactgacc ccttcgcca gatatccga caccacacg 
      gccctaagt tccatagac ttcaaacaa actctagac 
      ctaagcatg caaaaatta gaagaaagg aagcattct 
      tcagttaaa gattatagt gctctgtcg cttgtcggt 
      ggcacccac caccaatcc gaagaagaa gaagatgaa 
      gacgatgac ggaattgtc gtcgtaggt ggtgacgaa 
      gaagaagaa cccgagaag agtgaagtc acaaacggt 
      gtttccgac cagcctgaa gatttcagt atgccttcg 
      aaaaggccc aaaatgtca gtttccgac gtagaagaa 
      gacataact agtaattta gatagtaac gaagattcg 
      ctaagtatt gtcgacacc catagtctc aaagatgaa 
      ctaaatgta caaacgaac aaaagaaaa cgtaaaagt 
      caaaacccc acccgatgc gctgtacct gcaatgatg 
      gaagacgcc gtcagcgac tgcgactcc tccaacgac 
      gttttttcc gaatctctg aacgaaaaa acccacacg 
      aatgacgaa aaacgctca ccaagccgt ctcgaagcc 
      aagaaagaa gaagacaag tttgaagaa aaatgcgac 
      tcgccgcta aacctcgaa aaacgctgc cccgacgtg 
      aatgccaat gaacccgaa aaaatcgcg agtccggaa 
      ataaagaaa agtttggca cctttattc atgatcgat 
      aagttaaaa aaagaaaaa ccatcgcac gactcggag 
      ccggtcgaa gaaaccgat gagaggccc gccagttcg 
      gtcgaaagc aacaaaagt gacgagtca ttcgactcg 
      tccaacgct ttgcgacac ttggagagc ttgtcccac 
      gggcacttc ggcgacttg atggccaag ggcctccat 
      ctgggcctg gggcaaggc ccgcagttc ccccccatc 
      gcgttcatg atggggggc ggccctccc agtccagcc 
      cgctcccag gcctcgtct gcgggcagc agcaacggc 
      gcggaatct ccggacgaa aacagccag aaccagttt 
      tttgggcac tttgacaac ggccagttc atcagcgga 
      atggatgtt cctatcgat aaagacaac ccgaggaag 
      tgcaccgcg tgcggaaaa atcttccaa aaccacttc 
      ggcgtgaaa acgcattac caaaacgtg catctgaag 
      ctgatgcac aaatgcaac gtcgacgga tgcaatgcg 
      gcgtttccc tccaagcgg agtcgcgac agacatagt 
      gcaaattta aatttacat aggaagcta ttatctacc 
      tcttcggat aagtcggcg gcgagtttg ttcctggag 
      aagtctccc ttcgccctc cctggcgat ttcctgaca 
      aggctctac gcagaagaa gctctcaag aaccaccac 
      ttgccgccg ccgaatttg gaccagttg atgctcaac 
      ggcgatcga ataccccat ccccccctc ctgttgccc 
      cccctcggg ggcatcccc ttcccgctg gggagcttc 
      aaccacttc gcccagttc aacgggtct tcgacgatg 
      aaagagcgt tcgtcgcgg tcaagttcg ccggtttct 
      ggctcccct ccgccgaca aatccgtcg cccgtttcg 
      ctgaagtac acgcactgt gtggacgaa gaccaacca 
      actcccgat aaagacggg aatctccct tgtcggctg 
      tgcaaaact ccgtttccc acttcgtcc cagttgaaa 
      gaacattgc gaaagtaac cacctgtcc gaaatgttt 
      aaatgcacg gttatgggg tgtaataag gtatttatg 
      tcgcggacg cggcggaat ttgcacgcg gaaaatgag 
      tcgcttcat gtgagttcg tcgaggaag agttcggag 
      agttcgtga
    mRNA sequence
    • >TC001693 RA
      gttttactg tagtactat acatagttg ggcaaaatg 
      tatagcata gtaagatgg agcagataa taaagcagc 
      taacaagtt tctgatata tgttctggt attgtaaga 
      agtctcgag gtggacgta gccgtagtc gtatccgta 
      tcgtgagat ggtcaaagt aagatagcg tttgaattc 
      cgagtttga agcgatagg acagcacat gacgggggg 
      tgcaataca tgtcccact atgcccgtt caaggggta 
      tcgtcacct acaccgtgc gtccacctt ggcaccccc 
      ccaaaggag tcggctcct ccccgtttg cgcacgcaa 
      acttatccc cacttttgt tacagcact ggacaagtt 
      gggaacgcg acatctctt cagtaacag tgcccccga 
      accagtgca agtttacgc cgcgtttga catcgctag 
      tttagtcct ttacggatg tcaagccct cccaattcg 
      ccttaaaat actcctgga tagactgtt ttcggtgct 
      tcgcaaaga gcaagtgct tcaagtgtt gcatggatt 
      tggctggac cgccgaaga ttacacaag aggatatat 
      actgcagga gtcacacgg cggcactct ggaccgatg 
      gagcatctg cagtcctga ggaggagcc tctggtcct 
      ccaacagtt ccttaggtt tggagagac gcgagccat 
      aacgcaaca attgcttct ggcgcagca ggcacttca 
      ggatcccac actggagcg cctagaacg ccgccactc 
      gccaaacct tcgtcgggc gatgtcacc taaccatcg 
      cccgtccca ccaaactgt tccatctgc ttttcaccc 
      gtctcgctt accgacaat gtctcctca tcaaaaaca 
      tcacttaaa tttcttgaa gatgtctac ctccaacgg 
      acctctccc ctccaactc tcctccgag aagcgtcag 
      tactagtcc attgaacac gtcaccctt gaaccgtct 
      acaaaacat gcaaccttt cgatttccg taaattggg 
      tagcgggct gacgaattt tccgaaccg tgtaagtcc 
      cgacatgaa tcgaagaag ggcggaaag tgagagcgt 
      gggcttgaa tttgacgat ggccacgtc gttgtcttc 
      gtgtttgcc gccgccgcc tccacggcc cccgtcttt 
      gagccactc ggcggcggc aatggcagc cgctgtttc 
      ggtcaatcc cttggcggc ggccagttt ggtggcctc 
      gtccttctc gggacttat gtcatcgag gacaccgag 
      gagcaaatc accaagtgg ggaagtttt aagtggcaa 
      taataatag tgaagaaga cgatgatgt tgacgaaaa 
      ctcttatag tgcacttaa tttaagtcg tgatagaga 
      tattctcaa agccccaag acaaccgag gggaatccc 
      gggacgaaa acccacaac acctactaa aagacagtg 
      gggatcgcc gggattgcc tctgaatct aggaacgca 
      attcatcaa cccggccac agggaagaa aagagtgca 
      atgtaacgt atgtttaaa gacgttttg cgacaaagg 
      tgctcttaa aattcattt ctcggcggt gcatttacg 
      tgaaatgca caaatgtac ggttgaagg ttgtaatat 
      gatgtttag ttcaagaag gtcgagaaa tcgtcatag 
      tgctaatcc aaatccaaa attgcattc acctcatct 
      tcgtagaaa aatttcgcc gcacgatgg aagaagtgc 
      acaagctca tcctctcct aatacctcc ccaaactgg 
      tctttcact accagcagc agctgctgc cttgaatcc 
      gctcaatcc tttcggacc atttccctt actgacccc 
      ttcgccaga tatccgaca ccacacggc cctaagttc 
      catagactt caaacaaac tctagacct aagcatgca 
      aaaattaga agaaaggaa gcattcttc agttaaaga 
      ttatagtgc tctgtcgct tgtcggtgg cacccacca 
      ccaatccga agaagaaga agatgaaga cgatgacgg 
      aattgtcgt cgtaggtgg tgacgaaga agaagaacc 
      cgagaagag tgaagtcac aaacggtgt ttccgacca 
      gcctgaaga tttcagtat gccttcgaa aaggcccaa 
      aatgtcagt ttccgacgt agaagaaga cataactag 
      taatttaga tagtaacga agattcgct aagtattgt 
      cgacaccca tagtctcaa agatgaact aaatgtaca 
      aacgaacaa aagaaaacg taaaagtca aaaccccac 
      ccgatgcgc tgtacctgc aatgatgga agacgccgt 
      cagcgactg cgactcctc caacgacgt tttttccga 
      atctctgaa cgaaaaaac ccacacgaa tgacgaaaa 
      acgctcacc aagccgtct cgaagccaa gaaagaaga 
      agacaagtt tgaagaaaa atgcgactc gccgctaaa 
      cctcgaaaa acgctgccc cgacgtgaa tgccaatga 
      acccgaaaa aatcgcgag tccggaaat aaagaaaag 
      tttggcacc tttattcat gatcgataa gttaaaaaa 
      agaaaaacc atcgcacga ctcggagcc ggtcgaaga 
      aaccgatga gaggcccgc cagttcggt cgaaagcaa 
      caaaagtga cgagtcatt cgactcgtc caacgcttt 
      gcgacactt ggagagctt gtcccacgg gcacttcgg 
      cgacttgat ggccaaggg cctccatct gggcctggg 
      gcaaggccc gcagttccc ccccatcgc gttcatgat 
      ggggggcgg ccctcccag tccagcccg ctcccaggc 
      ctcgtctgc gggcagcag caacggcgc ggaatctcc 
      ggacgaaaa cagccagaa ccagttttt tgggcactt 
      tgacaacgg ccagttcat cagcggaat ggatgttcc 
      tatcgataa agacaaccc gaggaagtg caccgcgtg 
      cggaaaaat cttccaaaa ccacttcgg cgtgaaaac 
      gcattacca aaacgtgca tctgaagct gatgcacaa 
      atgcaacgt cgacggatg caatgcggc gtttccctc 
      caagcggag tcgcgacag acatagtgc aaatttaaa 
      tttacatag gaagctatt atctacctc ttcggataa 
      gtcggcggc gagtttgtt cctggagaa gtctccctt 
      cgccctccc tggcgattt cctgacaag gctctacgc 
      agaagaagc tctcaagaa ccaccactt gccgccgcc 
      gaatttgga ccagttgat gctcaacgg cgatcgaat 
      accccatcc ccccctcct gttgccccc cctcggggg 
      catcccctt cccgctggg gagcttcaa ccacttcgc 
      ccagttcaa cgggtcttc gacgatgaa agagcgttc 
      gtcgcggtc aagttcgcc ggtttctgg ctcccctcc 
      gccgacaaa tccgtcgcc cgtttcgct gaagtacac 
      gcactgtgt ggacgaaga ccaaccaac tcccgataa 
      agacgggaa tctcccttg tcggctgtg caaaactcc 
      gtttcccac ttcgtccca gttgaaaga acattgcga 
      aagtaacca cctgtccga aatgtttaa atgcacggt 
      tatggggtg taataaggt atttatgtc gcggacgcg 
      gcggaattt gcacgcgga aaatgagtc gcttcatgt 
      gagttcgtc gaggaagag ttcggagag ttcgtgacc 
      gttaagtaa ctttctgga cttgcggac tgtacccct 
      gtggtggtg atcgaccga tgtacttat caaatttta 
      ggtgttttc atatatgtg attactaga ataattagc 
      tctaaggtt cagcttata taatttatt ttttacttt 
      tacttgttt caacttcct ataaatcag ccagtcttg 
      ataatactc attgtatat aaaatattt atagaatta 
      ggtggtctc gtaaactgt tatagaaaa tcgcgctat 
      caatgtgaa tatttcaat tgctcattt tgataatac 
      tgcacaagt acgttaatt gtccaaatt gataattaa 
      ctcgcgcta aaagtccgc aagcttccg cgtttggac 
      agttggcag tgcttgact cactgttaa tgcatgcaa 
      atgaatatt cactctacc agtgacaac ttcagaatc 
      gaacgtgta tatttaaat gtgcaataa ctttgtaaa 
      tatgctatc ttgcctatg aatgtatag atgtatata 
      tttactatc attagtgaa aatttgccc tagattatc 
      ataaatgta tacgagaaa cctctgtcg attcgaatg 
      attttccac gccgttctc gggaagccc ctgaaccgt 
      ggccgttca aacccaaat taatgttat atttgtaat 
      atcttaaac agcacacct ctgtaaatg ttatttgtt 
      attgcatat tgcatattc acactccag tgtacagta 
      accattatg tatcgccta tgggcttca ttaaactta 
      tagctgtcg t
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY