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Gene information
General information
TC001680 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid65: 606370..609157
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001680
    iBeetle screen iB_03232
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001680 RA
      MEGVLWKWT NYWNGWQTR WFILENGVL 
      TYYKSQEEV NQGCRGSIK VQACEINVN 
      AIDNTRLDL VIPGEQHIY LKAATSQER 
      QQWLVALGS AKACVQKRT RNEPTEPNS 
      NALKKKKSE LRLYCDLLM QQVHVIKNA 
      NGDVEKMDD ATRLLGATC DTFIKTLEE 
      CMELSNANV MYNVVPNHD AILPPTKKI
    CDS sequence
    • >TC001680 RA
      atggaagga gttctttgg aaatggact aattactgg 
      aatgggtgg caaactcgc tggtttatt ctcgaaaac 
      ggagtactg acttactac aagtcgcaa gaagaagtt 
      aatcagggt tgtagaggt tccattaaa gtgcaagca 
      tgtgagatt aatgtaaac gccattgat aacacacgt 
      ttagacctt gtaattccc ggcgaacaa cacatttat 
      ctcaaagct gcaacatcg caagaaagg caacagtgg 
      ttagttgct ctagggagt gctaaagct tgtgtgcaa 
      aaaagaact cgaaacgaa cctactgag cctaatagc 
      aatgccttg aagaaaaag aaatcagag ttaaggctc 
      tattgtgat ttattgatg caacaagtg catgttatt 
      aaaaatgca aacggcgat gttgaaaaa atggatgat 
      gctacaaga ttgttagga gccacttgt gatactttc 
      attaagaca ttagaagaa tgcatggaa ttgtcaaat 
      gctaatgtt atgtataac gttgtgccc aatcatgac 
      gctatttta ccaccgaca aagaagata taa
    mRNA sequence
    • >TC001680 RA
      ttgttggtg tcactgctt gtgattgtc aaaaattga 
      aaacgtaaa cagataagt tctcattag taaatgtta 
      aatatgtgt tttaatttc ttataaatt ttatggttc 
      cgatacaaa aaggacaat ggaaggagt tctttggaa 
      atggactaa ttactggaa tgggtggca aactcgctg 
      gtttattct cgaaaacgg agtactgac ttactacaa 
      gtcgcaaga agaagttaa tcagggttg tagaggttc 
      cattaaagt gcaagcatg tgagattaa tgtaaacgc 
      cattgataa cacacgttt agaccttgt aattcccgg 
      cgaacaaca catttatct caaagctgc aacatcgca 
      agaaaggca acagtggtt agttgctct agggagtgc 
      taaagcttg tgtgcaaaa aagaactcg aaacgaacc 
      tactgagcc taatagcaa tgccttgaa gaaaaagaa 
      atcagagtt aaggctcta ttgtgattt attgatgca 
      acaagtgca tgttattaa aaatgcaaa cggcgatgt 
      tgaaaaaat ggatgatgc tacaagatt gttaggagc 
      cacttgtga tactttcat taagacatt agaagaatg 
      catggaatt gtcaaatgc taatgttat gtataacgt 
      tgtgcccaa tcatgacgc tattttacc accgacaaa 
      gaagatata aatgtactt cttactcaa agttctcat 
      ttctaattt ttttttcag aaataatgg atcttgttt 
      atgttttat gttaccgtc aaagccggt atttcatga 
      aatgactag tcttcgatt caatatact ttaactata 
      gttgtattg ttgggtgtt ccgtctaaa ctgaacaat 
      aattatcag ccgaaaaat tcattactg ctataattg 
      gtaaggttt atattgatt atggcattt ttagattat 
      ttttattta gagtagttg tcatgttta gattttttt 
      tttcaaaaa tctataacc actccttta catgctatt 
      gtggaatcg tatttgggt atagatcgt atacaaagg 
      gaaatatta ggtcttctt tagtgtatt cataattga 
      agagaaaat agttaacaa agttccaaa agttaatat 
      ttttttcca attttaaaa cacaaaagt tgagatcta 
      acctttttc tttctagta cgattttat aagagataa 
      ggaaaagct gatgaaaac ttctttgga ttgactcga 
      gtagtctaa ctactacta gagatgaat cttaaataa 
      tacaaaagt ggtaatttc gccaaattg caaatataa 
      tctttgcac tgcctagta tgtttagcc attttgaat 
      attttttga tgtctattt acctcctct tcaaaaata 
      tattataac tctttcatt tgatgatgt tatgaaatc 
      gtacttggg tttagaagg ggaaaggtt aggtttttt 
      ttagtgcat tcataacgg aataaaaca gtttacaat 
      tttccgaat tgttttttt acaatttag aaaacacaa 
      aagatgaaa tctaacctt tttctttct agtacgtat 
      aaaaatgct gatcacaaa tctgttaag tgtttcaaa 
      aatggctag actgaaggg aagactgat taaaactct 
      aaaaattca ctcgagtaa tctaaaaat gcagtcaaa 
      agctagttt tctatttaa ctctttagt gtccaagta 
      tcgaaaaat aatgagaca aatagtttc caagatgaa 
      cgaacatac ttacatcct caagaacgt tgtaattat 
      attttaata ttcttttaa ttgctgttg aatgctatt 
      agagatgaa tcctaagcg ctggctctt gtaaatgca 
      aaaactggt tatttcttc aaattgcaa tttgtattt 
      ataatcttc gcattgata aatgcaatg tctagaatg 
      tttagaaat ttatttttt tatgtgcac ttacttctt 
      caaaaatgt aggtattac atttccttt tgatgatat 
      catgaaatc gtacttggg tgttaaaag ggaaaggtt 
      aggtcttct ttagcttat ccataattg aataaaata 
      ctagttaac aaacttcag attttatca tttttttca 
      tctgaaaat ctttaattt tgtatttga ttgcaataa 
      ttatctttt tttcgtgtt tttttgcca tcattggag 
      ggaagtctt cttttctgc ttctgtttg ttccgcaac 
      ttaatttat ttttcggtc taagcgtcg tctctagaa 
      gtagttgcc tgcataatt gaatttttt gaagggaat 
      tatcttctc ttgaacgtt tttttaaga ggaatcaac 
      gtattttct ttccccatt tttcttttt cgggaggaa 
      atatcatac ttacatgga ataaatgga aggtggaaa 
      attatatat tctactgaa accattgta caaagcgat 
      cgaaaagtc cttctatca agtaattta gaaattaaa 
      ggacgtatg tctatagaa ctcaagcct aatgtcaaa 
      agaaaaatg tcaaaaaaa t