| General information | |||
| TC031231
Former TC numbers If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene. |
Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 2843913..2865157 | |
| Sequence information | |||
| Transcripts / Proteins (2) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC031231 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03230 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03231 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC031231 RA
MILSRKHVA WSAPTLFRR PFILATDAS GIAISAILS QKYEIEHPI CYASRSLKD AETRYSTIE REALAIVWA VKYFRCYLT GARFTIITD HRPLKYLLT IKEPSSRLA KWAMSLTEF DFEVQYRPG KQHHVDAFT RLEHPQEEM SIQAIQQDC TPIIYNLEE IKKAQSEDK DIQSLLPQE GYYQSPNGL CYRERLDGE RHDKLLVPK TMQRRILQL YHDSPFMAH GGRTKTTEL LKREFYWKG KRHGIRTHG TPYSKGYQN HHENWYPVI QPIADHPWL INLTIATQE PHPLPGSWD VTWRETESK RWGIVVTQD FPIPLAFKC GTEYEIQAV RRSPQSFTT DYTQTILYN QPCPKVPDT SFSNFLKYP VHTVPWILP VLLTFTALV FLLWLLLLG HIIKSSRQG KTHNVQNQN QGPTYHRCS SIAPDPEDP PNETTPTRV DVPYDVPHS PPRAVFTLL PHFTPEKAI EAIDILAKL QPPPKHPHH RCRCVKKLP KIVEEVHYA NIGECKEVS VCKSDRARG FFLLDLRDK IVDLLLGNH NVTPHFRSG CSKIALSGT LLLGVAVRV VVRKKGRHP GVDPTPSSH LVGIKMNFE DVTTKMVDA MTQTTGITI GEERSFADF TELAEREWT EVHYVKVKM TAISPLDMS EEVSPAIII SRDKNDERG LLRSFLLRF NPVLREVMC ESENPLKFE VVSIKQRVT LTEPNESPQ PPKDTYMFV IVGDELEVA NGSLEKTYH SLVELKNRV KLLPVKQIA VACDLRHRP EYHRKMLEY IFRERKEDC KMDTVMISG RAPMITYDS TLREVREHL KDTCSITSV TWINSGKFL FQVEKGLGR IVKEILLSK MGQYWIAWK GPNTQFRRP EKKQDVFCY KCWELGHLG TECKGVDRR HLCQWCGEE GHTRKECTN HYFCPVCNQ NKHRARTPR CGNTQKKAQ KAAECTENT KTIQTEERS FLEFRKLVR SEWSDTVEY EKVKTLAVS PFDMPADVA TAIIISRLQ KEEGALLRS LYERYPELR VEMRECGKV MEYGVKYLR QSKVCVPHE DSYVFVVVA NALKGAVGK LQRTVNALL DLNEEVRRA GIAKIAVAC DQMFKLDYH RKMLEYIFR DEEVEVTFC TPLSGEEVD ISLPKKDKP SHDTIFITG HGRNVTYRS LLLEVREHL KDFESVITE IKKVHRRKL LMQIEKGQG SYISEILRS KMSQHVIYL RAVEQPPYD GFCSKCWEY GHTRKTCQG LNRFYNCRR TGLVWIFKL QDTLRLLPL NLIFEAFFV LYVVPYQEH GILSVLLLS ILQIDCLQI IARSSRYTS HRYLNQGAQ NLFIKIKDR CTNSNHQNG ACHANFSGN CLVRNLGAT FK
- >TC031231 RB
MILSRKHVA WSAPTLFRR PFILATDAS GIAISAILS QKYEIEHPI CYASRSLKD AETRYSTIE REALAIVWA VKYFRCYLT GARFTIITD HRPLKYLLT IKEPSSRLA KWAMSLTEF DFEVQYRPG KQHHVDAFT RLEHPQEEM SIQAIQQDC TPIIYNLEE IKKAQSEDK DIQSLLPQE GYYQSPNGL CYRERLDGE RHDKLLVPK TMQRRILQL YHDSPFMAH GGRTKTTEL LKREFYWKV IQPIADHPW LINLTIATQ EPHPLPGSW DVTWRETES KRWGIVVTQ DFPIPLAFK CGTEYEIQA VRRSPQSFT TDYTQTILY NQPCPKVPD TSFSNFLKY PVHTVPWIL PVLLTFTAL VFLLWLLLL GHIIKSSRQ GKTHNVQNQ NQGPTYHRC SSIAPDPED PPNETTPTR VDVPYDVPH SPPRAVFTL LPHFTPEKA IEAIDILAK LQPPPKHPH HRCRCVKKL PKIVEEVHY ANIGECKEV SVCKSDRAR GFFLLDLRD KIVDLLLGN HNVTPHFRS GCSKIALSG TLLLGVAVR VVVRKKGRH PGVDPTPSS HLVGIKMNF EDVTTKMVD AMTQTTGIT IGEERSFAD FTELAEREW TEVHYVKVK MTAISPLDM SEEVSPAII ISRDKNDER GLLRSFLLR FNPVLREVM CESENPLKF EVVSIKQRV TLTEPNESP QPPKDTYMF VIVGDELEV ANGSLEKTY HSLVELKNR VKLLPVKQI AVACDLRHR PEYHRKMLE YIFRERKED CKMDTVMIS GRAPMITYD STLREVREH LKDTCSITS VTWINSGKF LFQVEKGLG RIVKEILLS KMGQYWIAW KGPNTQFRR PEKKQDVFC YKCWELGHL GTECKGVDR RHLCQWCGE EGHTRKECT NHYFCPVCN QNKHRARTP RCGNTQKKA QKAAECTEN TKTIQTEER SFLEFRKLV RSEWSDTVE YEKVKTLAV SPFDMPADV ATAIIISRL QKEEGALLR SLYERYPEL RVEMRECGK VMEYGVKYL RQSKVCVPH EDSYVFVVV ANALKGAVG KLQRTVNAL LDLNEEVRR AGIAKIAVA CDQMFKLDY HRKMLEYIF RDEEVEVTF CTPLSGEEV DISLPKKDK PSHDTIFIT GHGRNVTYR SLLLEVREH LKDFESVIT EIKKVHRRK LLMQIEKGQ GSYISEILR SKMSQHVIY LRAVEQPPY DGFCSKCWE YGHTRKTCQ GLNRFYNCR RTGLVWIFK LQDTLRLLP LNLIFEAFF VLYVVPYQE HGILSVLLL SILQIDCLQ IIARSSRYT SHRYLNQGA QNLFIKIKD RCTNSNHQN GACHANFSG NCLVRNLGA TFK
- >TC031231 RA
atgatatta tcaagaaag cacgtcgct tggtcagct ccgactttg tttcgacgg cctttcatt ttagcaaca gatgcgtcg ggaattgct atcagtgca attttatcg caaaaatac gaaatcgaa catccaatt tgttatgca agcagaagc ttgaaggac gcggagacc aggtacagc acgattgaa cgggaggcg ttggccatt gtttgggct gtcaaatac tttcgttgt tatttgaca ggtgctcga tttactata attacggac catcgacct ttaaaatac ctcttaaca ataaaagaa cctagttca cgcttagct aaatgggct atgtcctta accgagttc gactttgaa gttcaatac cgtcccgga aaacaacat catgttgat gcttttacc cgattagaa cacccgcaa gaggaaatg tcgattcaa gccatacaa caggattgt actccaatt atctataat ctggaggag atcaagaaa gcccaaagc gaagataag gacattcaa tccttgtta ccacaagag ggatactac caatctcct aatggatta tgctacagg gaacggctg gatggagag cgacatgat aaattattg gtgcccaaa acaatgcaa agaagaatc cttcaattg taccatgat tctcctttt atggcccat ggtggacgc accaaaaca actgaatta ctcaagcga gagttttat tggaagggg aagcgtcat ggaattaga acccatggg acaccatac tcaaagggt taccaaaac caccatgag aattggtat ccagtgatc caacctatc gcggaccac ccttggtta atcaactta accattgcc acacaagag ccacaccct ttgccaggc tcatgggat gttacttgg agggaaact gaatctaag agatggggc attgtggtt acgcaagat ttccccata ccactcgct ttcaaatgt ggaaccgaa tacgagatc caggcagtg agaagaagt cctcaatcc tttacaacg gattatacg caaactatt ctctataat caaccctgt ccaaaggtt ccagacacc tcattcagt aacttcttg aaataccct gttcataca gtgccgtgg attcttcca gttcttctc acgtttact gcattagtg ttcttgcta tggttgctt ttattgggc cacataatc aaaagctca cgacaggga aagacacac aatgttcaa aaccaaaat cagggtcct acgtatcat cgttgctcc agcatagca cctgaccca gaggaccca ccaaacgaa acaacccca accagagtg gatgtacca tacgatgta ccgcattct ccaccaaga gctgtgttc actctgcta ccccacttc actccagag aaagcgata gaagccatt gatatatta gccaaacta cagcctcca ccaaaacac cctcaccac cgttgccga tgtgtaaaa aaactaccc aaaattgtt gaagaagtt cactacgca aacattggt gagtgcaaa gaagtgagt gtgtgtaaa agtgaccgc gcccggggc ttttttctt ttggatttg cgagacaaa attgtcgac ctcctcctc ggtaatcac aacgtcact ccccacttc cgcagtggt tgttcgaaa atcgctttg agtggcacg ttgctcctc ggtgttgcc gtcagggtc gtcgtccgg aagaagggg cgtcaccct ggggtggac cccacacca tcgtcccat ctcgtggga ataaaaatg aattttgaa gatgttaca acgaaaatg gttgatgcc atgacacaa acgacggga ataaccata ggagaagag aggtccttt gccgatttt accgaattg gccgaacgt gaatggacg gaggtccat tacgtgaaa gttaaaatg acggcaatt agtccgctt gatatgtca gaagaggta tctccggct attatcata tctagggac aaaaatgac gaaagggga ttgctgcgc tcgttttta cttcgattt aatccggtt ttgagagag gttatgtgc gaaagtgag aacccctta aagtttgaa gtcgtatcg ataaagcaa agagtaacg ttgacggaa cccaacgaa agtccccaa cctccaaaa gacacgtat atgtttgtg atagtcggt gatgagcta gaagtggca aatggatca ctggaaaaa acataccac tctctagtc gagctcaaa aacagagta aagctattg ccagtcaaa caaatcgct gtggcttgc gatcttaga cacagacct gaataccac cggaagatg ctggagtac atcttcaga gagcgaaaa gaggactgt aaaatggat actgtcatg ataagtggc cgcgcaccg atgattact tacgattcc acgttgcgt gaagtacgt gaacacttg aaggacact tgcagtatc acttctgta acgtggata aattcaggc aaattcttg ttccaagtg gagaaaggg ctaggaagg attgttaag gagatcctc ctctctaaa atgggccaa tattggatc gcatggaag ggtcctaat acacaattc cgacgtccc gaaaagaag caagacgtt ttctgttac aaatgttgg gagttgggt catttgggc acggagtgt aagggtgtc gatagaaga catttgtgt cagtggtgt ggcgaggaa ggccatacc aggaaagag tgcacaaat cactacttc tgtccagtg tgcaaccaa aacaaacac agggcgagg acaccaaga tgtggaaac acccaaaag aaggcacag aaagccgcc gaatgtacg gaaaataca aaaacgatt cagacagaa gagcgatcc tttcttgaa tttaggaag ttggtcagg agtgagtgg agcgatacc gttgagtac gagaaagtt aaaacgctc gctgtaagt ccgtttgac atgcctgca gacgtagcc acggccata atcatatcg cgactgcaa aaggaggaa ggagctttg ttacgctcg ttgtacgag cgttatcca gaattgaga gtggaaatg cgtgaatgc ggcaaggta atggagtat ggagttaag tacttgagg caaagcaaa gtgtgtgta ccacatgag gacagctac gtgtttgtg gtagtagca aacgcattg aaaggggca gtcggtaaa ttacaaaga acagttaat gctctactg gacctgaat gaggaagtt agaagagcg ggaatcgct aaaatcgct gtggcttgc gaccagatg ttcaaactc gattatcac aggaagatg cttgaatat atcttccga gacgaggaa gtggaagtc actttttgc actcccctc tctggcgaa gaagttgac atttcgctt cctaagaaa gataaaccg agtcatgat accatcttt atcactggt catgggagg aatgttaca tacagatcg ctcttactt gaagtgcgt gaacacttg aaagacttt gaatccgtc atcaccgaa ataaagaaa gttcacaga aggaaactc ttaatgcag attgagaaa gggcaaggt tcgtacatt tcggagatc cttcgctca aaaatgagc caacatgtc atctattta agagcggtg gagcaaccg ccgtacgat ggtttctgt tccaagtgt tgggaatat ggccatacg cgtaagact tgccaaggt ttgaacaga ttttataat tgccgcaga acgggtctt gtgtggata tttaagctg caggatact ttgagatta cttcctttg aatttaatt tttgaagct ttcttcgtt ctgtatgta gttccttac caagaacat ggaatactc agcgtgtta cttctgagc attctgcag attgactgt ctacaaatt attgctagg tcgtcaaga tatacttca catagatac ctcaatcaa ggtgcacag aacttgttc atcaagata aaagatcgg tgtaccaac tctaatcat cagaatgga gcctgtcat gccaacttc agtggtaat tgtctagtc aggaattta ggagctaca tttaaataa
- >TC031231 RB
atgatatta tcaagaaag cacgtcgct tggtcagct ccgactttg tttcgacgg cctttcatt ttagcaaca gatgcgtcg ggaattgct atcagtgca attttatcg caaaaatac gaaatcgaa catccaatt tgttatgca agcagaagc ttgaaggac gcggagacc aggtacagc acgattgaa cgggaggcg ttggccatt gtttgggct gtcaaatac tttcgttgt tatttgaca ggtgctcga tttactata attacggac catcgacct ttaaaatac ctcttaaca ataaaagaa cctagttca cgcttagct aaatgggct atgtcctta accgagttc gactttgaa gttcaatac cgtcccgga aaacaacat catgttgat gcttttacc cgattagaa cacccgcaa gaggaaatg tcgattcaa gccatacaa caggattgt actccaatt atctataat ctggaggag atcaagaaa gcccaaagc gaagataag gacattcaa tccttgtta ccacaagag ggatactac caatctcct aatggatta tgctacagg gaacggctg gatggagag cgacatgat aaattattg gtgcccaaa acaatgcaa agaagaatc cttcaattg taccatgat tctcctttt atggcccat ggtggacgc accaaaaca actgaatta ctcaagcga gagttttat tggaaggtg atccaacct atcgcggac cacccttgg ttaatcaac ttaaccatt gccacacaa gagccacac cctttgcca ggctcatgg gatgttact tggagggaa actgaatct aagagatgg ggcattgtg gttacgcaa gatttcccc ataccactc gctttcaaa tgtggaacc gaatacgag atccaggca gtgagaaga agtcctcaa tcctttaca acggattat acgcaaact attctctat aatcaaccc tgtccaaag gttccagac acctcattc agtaacttc ttgaaatac cctgttcat acagtgccg tggattctt ccagttctt ctcacgttt actgcatta gtgttcttg ctatggttg cttttattg ggccacata atcaaaagc tcacgacag ggaaagaca cacaatgtt caaaaccaa aatcagggt cctacgtat catcgttgc tccagcata gcacctgac ccagaggac ccaccaaac gaaacaacc ccaaccaga gtggatgta ccatacgat gtaccgcat tctccacca agagctgtg ttcactctg ctaccccac ttcactcca gagaaagcg atagaagcc attgatata ttagccaaa ctacagcct ccaccaaaa caccctcac caccgttgc cgatgtgta aaaaaacta cccaaaatt gttgaagaa gttcactac gcaaacatt ggtgagtgc aaagaagtg agtgtgtgt aaaagtgac cgcgcccgg ggctttttt cttttggat ttgcgagac aaaattgtc gacctcctc ctcggtaat cacaacgtc actccccac ttccgcagt ggttgttcg aaaatcgct ttgagtggc acgttgctc ctcggtgtt gccgtcagg gtcgtcgtc cggaagaag gggcgtcac cctggggtg gaccccaca ccatcgtcc catctcgtg ggaataaaa atgaatttt gaagatgtt acaacgaaa atggttgat gccatgaca caaacgacg ggaataacc ataggagaa gagaggtcc tttgccgat tttaccgaa ttggccgaa cgtgaatgg acggaggtc cattacgtg aaagttaaa atgacggca attagtccg cttgatatg tcagaagag gtatctccg gctattatc atatctagg gacaaaaat gacgaaagg ggattgctg cgctcgttt ttacttcga tttaatccg gttttgaga gaggttatg tgcgaaagt gagaacccc ttaaagttt gaagtcgta tcgataaag caaagagta acgttgacg gaacccaac gaaagtccc caacctcca aaagacacg tatatgttt gtgatagtc ggtgatgag ctagaagtg gcaaatgga tcactggaa aaaacatac cactctcta gtcgagctc aaaaacaga gtaaagcta ttgccagtc aaacaaatc gctgtggct tgcgatctt agacacaga cctgaatac caccggaag atgctggag tacatcttc agagagcga aaagaggac tgtaaaatg gatactgtc atgataagt ggccgcgca ccgatgatt acttacgat tccacgttg cgtgaagta cgtgaacac ttgaaggac acttgcagt atcacttct gtaacgtgg ataaattca ggcaaattc ttgttccaa gtggagaaa gggctagga aggattgtt aaggagatc ctcctctct aaaatgggc caatattgg atcgcatgg aagggtcct aatacacaa ttccgacgt cccgaaaag aagcaagac gttttctgt tacaaatgt tgggagttg ggtcatttg ggcacggag tgtaagggt gtcgataga agacatttg tgtcagtgg tgtggcgag gaaggccat accaggaaa gagtgcaca aatcactac ttctgtcca gtgtgcaac caaaacaaa cacagggcg aggacacca agatgtgga aacacccaa aagaaggca cagaaagcc gccgaatgt acggaaaat acaaaaacg attcagaca gaagagcga tcctttctt gaatttagg aagttggtc aggagtgag tggagcgat accgttgag tacgagaaa gttaaaacg ctcgctgta agtccgttt gacatgcct gcagacgta gccacggcc ataatcata tcgcgactg caaaaggag gaaggagct ttgttacgc tcgttgtac gagcgttat ccagaattg agagtggaa atgcgtgaa tgcggcaag gtaatggag tatggagtt aagtacttg aggcaaagc aaagtgtgt gtaccacat gaggacagc tacgtgttt gtggtagta gcaaacgca ttgaaaggg gcagtcggt aaattacaa agaacagtt aatgctcta ctggacctg aatgaggaa gttagaaga gcgggaatc gctaaaatc gctgtggct tgcgaccag atgttcaaa ctcgattat cacaggaag atgcttgaa tatatcttc cgagacgag gaagtggaa gtcactttt tgcactccc ctctctggc gaagaagtt gacatttcg cttcctaag aaagataaa ccgagtcat gataccatc tttatcact ggtcatggg aggaatgtt acatacaga tcgctctta cttgaagtg cgtgaacac ttgaaagac tttgaatcc gtcatcacc gaaataaag aaagttcac agaaggaaa ctcttaatg cagattgag aaagggcaa ggttcgtac atttcggag atccttcgc tcaaaaatg agccaacat gtcatctat ttaagagcg gtggagcaa ccgccgtac gatggtttc tgttccaag tgttgggaa tatggccat acgcgtaag acttgccaa ggtttgaac agattttat aattgccgc agaacgggt cttgtgtgg atatttaag ctgcaggat actttgaga ttacttcct ttgaattta atttttgaa gctttcttc gttctgtat gtagttcct taccaagaa catggaata ctcagcgtg ttacttctg agcattctg cagattgac tgtctacaa attattgct aggtcgtca agatatact tcacataga tacctcaat caaggtgca cagaacttg ttcatcaag ataaaagat cggtgtacc aactctaat catcagaat ggagcctgt catgccaac ttcagtggt aattgtcta gtcaggaat ttaggagct acatttaaa taa
- >TC031231 RA
taaggaatc caaaacttg ctggacaat gatattatc aagaaagca cgtcgcttg gtcagctcc gactttgtt tcgacggcc tttcatttt agcaacaga tgcgtcggg aattgctat cagtgcaat tttatcgca aaaatacga aatcgaaca tccaatttg ttatgcaag cagaagctt gaaggacgc ggagaccag gtacagcac gattgaacg ggaggcgtt ggccattgt ttgggctgt caaatactt tcgttgtta tttgacagg tgctcgatt tactataat tacggacca tcgaccttt aaaatacct cttaacaat aaaagaacc tagttcacg cttagctaa atgggctat gtccttaac cgagttcga ctttgaagt tcaataccg tcccggaaa acaacatca tgttgatgc ttttacccg attagaaca cccgcaaga ggaaatgtc gattcaagc catacaaca ggattgtac tccaattat ctataatct ggaggagat caagaaagc ccaaagcga agataagga cattcaatc cttgttacc acaagaggg atactacca atctcctaa tggattatg ctacaggga acggctgga tggagagcg acatgataa attattggt gcccaaaac aatgcaaag aagaatcct tcaattgta ccatgattc tccttttat ggcccatgg tggacgcac caaaacaac tgaattact caagcgaga gttttattg gaaggggaa gcgtcatgg aattagaac ccatgggac accatactc aaagggtta ccaaaacca ccatgagaa ttggtatcc agtgatcca acctatcgc ggaccaccc ttggttaat caacttaac cattgccac acaagagcc acacccttt gccaggctc atgggatgt tacttggag ggaaactga atctaagag atggggcat tgtggttac gcaagattt ccccatacc actcgcttt caaatgtgg aaccgaata cgagatcca ggcagtgag aagaagtcc tcaatcctt tacaacgga ttatacgca aactattct ctataatca accctgtcc aaaggttcc agacacctc attcagtaa cttcttgaa ataccctgt tcatacagt gccgtggat tcttccagt tcttctcac gtttactgc attagtgtt cttgctatg gttgctttt attgggcca cataatcaa aagctcacg acagggaaa gacacacaa tgttcaaaa ccaaaatca gggtcctac gtatcatcg ttgctccag catagcacc tgacccaga ggacccacc aaacgaaac aaccccaac cagagtgga tgtaccata cgatgtacc gcattctcc accaagagc tgtgttcac tctgctacc ccacttcac tccagagaa agcgataga agccattga tatattagc caaactaca gcctccacc aaaacaccc tcaccaccg ttgccgatg tgtaaaaaa actacccaa aattgttga agaagttca ctacgcaaa cattggtga gtgcaaaga agtgagtgt gtgtaaaag tgaccgcgc ccggggctt ttttctttt ggatttgcg agacaaaat tgtcgacct cctcctcgg taatcacaa cgtcactcc ccacttccg cagtggttg ttcgaaaat cgctttgag tggcacgtt gctcctcgg tgttgccgt cagggtcgt cgtccggaa gaaggggcg tcaccctgg ggtggaccc cacaccatc gtcccatct cgtgggaat aaaaatgaa ttttgaaga tgttacaac gaaaatggt tgatgccat gacacaaac gacgggaat aaccatagg agaagagag gtcctttgc cgattttac cgaattggc cgaacgtga atggacgga ggtccatta cgtgaaagt taaaatgac ggcaattag tccgcttga tatgtcaga agaggtatc tccggctat tatcatatc tagggacaa aaatgacga aaggggatt gctgcgctc gtttttact tcgatttaa tccggtttt gagagaggt tatgtgcga aagtgagaa ccccttaaa gtttgaagt cgtatcgat aaagcaaag agtaacgtt gacggaacc caacgaaag tccccaacc tccaaaaga cacgtatat gtttgtgat agtcggtga tgagctaga agtggcaaa tggatcact ggaaaaaac ataccactc tctagtcga gctcaaaaa cagagtaaa gctattgcc agtcaaaca aatcgctgt ggcttgcga tcttagaca cagacctga ataccaccg gaagatgct ggagtacat cttcagaga gcgaaaaga ggactgtaa aatggatac tgtcatgat aagtggccg cgcaccgat gattactta cgattccac gttgcgtga agtacgtga acacttgaa ggacacttg cagtatcac ttctgtaac gtggataaa ttcaggcaa attcttgtt ccaagtgga gaaagggct aggaaggat tgttaagga gatcctcct ctctaaaat gggccaata ttggatcgc atggaaggg tcctaatac acaattccg acgtcccga aaagaagca agacgtttt ctgttacaa atgttggga gttgggtca tttgggcac ggagtgtaa gggtgtcga tagaagaca tttgtgtca gtggtgtgg cgaggaagg ccataccag gaaagagtg cacaaatca ctacttctg tccagtgtg caaccaaaa caaacacag ggcgaggac accaagatg tggaaacac ccaaaagaa ggcacagaa agccgccga atgtacgga aaatacaaa aacgattca gacagaaga gcgatcctt tcttgaatt taggaagtt ggtcaggag tgagtggag cgataccgt tgagtacga gaaagttaa aacgctcgc tgtaagtcc gtttgacat gcctgcaga cgtagccac ggccataat catatcgcg actgcaaaa ggaggaagg agctttgtt acgctcgtt gtacgagcg ttatccaga attgagagt ggaaatgcg tgaatgcgg caaggtaat ggagtatgg agttaagta cttgaggca aagcaaagt gtgtgtacc acatgagga cagctacgt gtttgtggt agtagcaaa cgcattgaa aggggcagt cggtaaatt acaaagaac agttaatgc tctactgga cctgaatga ggaagttag aagagcggg aatcgctaa aatcgctgt ggcttgcga ccagatgtt caaactcga ttatcacag gaagatgct tgaatatat cttccgaga cgaggaagt ggaagtcac tttttgcac tcccctctc tggcgaaga agttgacat ttcgcttcc taagaaaga taaaccgag tcatgatac catctttat cactggtca tgggaggaa tgttacata cagatcgct cttacttga agtgcgtga acacttgaa agactttga atccgtcat caccgaaat aaagaaagt tcacagaag gaaactctt aatgcagat tgagaaagg gcaaggttc gtacatttc ggagatcct tcgctcaaa aatgagcca acatgtcat ctatttaag agcggtgga gcaaccgcc gtacgatgg tttctgttc caagtgttg ggaatatgg ccatacgcg taagacttg ccaaggttt gaacagatt ttataattg ccgcagaac gggtcttgt gtggatatt taagctgca ggatacttt gagattact tcctttgaa tttaatttt tgaagcttt cttcgttct gtatgtagt tccttacca agaacatgg aatactcag cgtgttact tctgagcat tctgcagat tgactgtct acaaattat tgctaggtc gtcaagata tacttcaca tagatacct caatcaagg tgcacagaa cttgttcat caagataaa agatcggtg taccaactc taatcatca gaatggagc ctgtcatgc caacttcag tggtaattg tctagtcag gaatttagg agctacatt taaataaac tcaaaaaac cgcaaattt ttgaagcat ttaatatgg ggagtttga agttttgtg agatttttt atttatttg tttataact tgtttagtt ttattcttg taataagta aatagatta tctttgcta cgagaagta aacataaaa tttactgat ttatttgtt attttcaaa ttgtaaatg aaagtgaat aaaggacct cactagta
- >TC031231 RB
taaggaatc caaaacttg ctggacaat gatattatc aagaaagca cgtcgcttg gtcagctcc gactttgtt tcgacggcc tttcatttt agcaacaga tgcgtcggg aattgctat cagtgcaat tttatcgca aaaatacga aatcgaaca tccaatttg ttatgcaag cagaagctt gaaggacgc ggagaccag gtacagcac gattgaacg ggaggcgtt ggccattgt ttgggctgt caaatactt tcgttgtta tttgacagg tgctcgatt tactataat tacggacca tcgaccttt aaaatacct cttaacaat aaaagaacc tagttcacg cttagctaa atgggctat gtccttaac cgagttcga ctttgaagt tcaataccg tcccggaaa acaacatca tgttgatgc ttttacccg attagaaca cccgcaaga ggaaatgtc gattcaagc catacaaca ggattgtac tccaattat ctataatct ggaggagat caagaaagc ccaaagcga agataagga cattcaatc cttgttacc acaagaggg atactacca atctcctaa tggattatg ctacaggga acggctgga tggagagcg acatgataa attattggt gcccaaaac aatgcaaag aagaatcct tcaattgta ccatgattc tccttttat ggcccatgg tggacgcac caaaacaac tgaattact caagcgaga gttttattg gaaggtgat ccaacctat cgcggacca cccttggtt aatcaactt aaccattgc cacacaaga gccacaccc tttgccagg ctcatggga tgttacttg gagggaaac tgaatctaa gagatgggg cattgtggt tacgcaaga tttccccat accactcgc tttcaaatg tggaaccga atacgagat ccaggcagt gagaagaag tcctcaatc ctttacaac ggattatac gcaaactat tctctataa tcaaccctg tccaaaggt tccagacac ctcattcag taacttctt gaaataccc tgttcatac agtgccgtg gattcttcc agttcttct cacgtttac tgcattagt gttcttgct atggttgct tttattggg ccacataat caaaagctc acgacaggg aaagacaca caatgttca aaaccaaaa tcagggtcc tacgtatca tcgttgctc cagcatagc acctgaccc agaggaccc accaaacga aacaacccc aaccagagt ggatgtacc atacgatgt accgcattc tccaccaag agctgtgtt cactctgct accccactt cactccaga gaaagcgat agaagccat tgatatatt agccaaact acagcctcc accaaaaca ccctcacca ccgttgccg atgtgtaaa aaaactacc caaaattgt tgaagaagt tcactacgc aaacattgg tgagtgcaa agaagtgag tgtgtgtaa aagtgaccg cgcccgggg cttttttct tttggattt gcgagacaa aattgtcga cctcctcct cggtaatca caacgtcac tccccactt ccgcagtgg ttgttcgaa aatcgcttt gagtggcac gttgctcct cggtgttgc cgtcagggt cgtcgtccg gaagaaggg gcgtcaccc tggggtgga ccccacacc atcgtccca tctcgtggg aataaaaat gaattttga agatgttac aacgaaaat ggttgatgc catgacaca aacgacggg aataaccat aggagaaga gaggtcctt tgccgattt taccgaatt ggccgaacg tgaatggac ggaggtcca ttacgtgaa agttaaaat gacggcaat tagtccgct tgatatgtc agaagaggt atctccggc tattatcat atctaggga caaaaatga cgaaagggg attgctgcg ctcgttttt acttcgatt taatccggt tttgagaga ggttatgtg cgaaagtga gaacccctt aaagtttga agtcgtatc gataaagca aagagtaac gttgacgga acccaacga aagtcccca acctccaaa agacacgta tatgtttgt gatagtcgg tgatgagct agaagtggc aaatggatc actggaaaa aacatacca ctctctagt cgagctcaa aaacagagt aaagctatt gccagtcaa acaaatcgc tgtggcttg cgatcttag acacagacc tgaatacca ccggaagat gctggagta catcttcag agagcgaaa agaggactg taaaatgga tactgtcat gataagtgg ccgcgcacc gatgattac ttacgattc cacgttgcg tgaagtacg tgaacactt gaaggacac ttgcagtat cacttctgt aacgtggat aaattcagg caaattctt gttccaagt ggagaaagg gctaggaag gattgttaa ggagatcct cctctctaa aatgggcca atattggat cgcatggaa gggtcctaa tacacaatt ccgacgtcc cgaaaagaa gcaagacgt tttctgtta caaatgttg ggagttggg tcatttggg cacggagtg taagggtgt cgatagaag acatttgtg tcagtggtg tggcgagga aggccatac caggaaaga gtgcacaaa tcactactt ctgtccagt gtgcaacca aaacaaaca cagggcgag gacaccaag atgtggaaa cacccaaaa gaaggcaca gaaagccgc cgaatgtac ggaaaatac aaaaacgat tcagacaga agagcgatc ctttcttga atttaggaa gttggtcag gagtgagtg gagcgatac cgttgagta cgagaaagt taaaacgct cgctgtaag tccgtttga catgcctgc agacgtagc cacggccat aatcatatc gcgactgca aaaggagga aggagcttt gttacgctc gttgtacga gcgttatcc agaattgag agtggaaat gcgtgaatg cggcaaggt aatggagta tggagttaa gtacttgag gcaaagcaa agtgtgtgt accacatga ggacagcta cgtgtttgt ggtagtagc aaacgcatt gaaaggggc agtcggtaa attacaaag aacagttaa tgctctact ggacctgaa tgaggaagt tagaagagc gggaatcgc taaaatcgc tgtggcttg cgaccagat gttcaaact cgattatca caggaagat gcttgaata tatcttccg agacgagga agtggaagt cactttttg cactcccct ctctggcga agaagttga catttcgct tcctaagaa agataaacc gagtcatga taccatctt tatcactgg tcatgggag gaatgttac atacagatc gctcttact tgaagtgcg tgaacactt gaaagactt tgaatccgt catcaccga aataaagaa agttcacag aaggaaact cttaatgca gattgagaa agggcaagg ttcgtacat ttcggagat ccttcgctc aaaaatgag ccaacatgt catctattt aagagcggt ggagcaacc gccgtacga tggtttctg ttccaagtg ttgggaata tggccatac gcgtaagac ttgccaagg tttgaacag attttataa ttgccgcag aacgggtct tgtgtggat atttaagct gcaggatac tttgagatt acttccttt gaatttaat ttttgaagc tttcttcgt tctgtatgt agttcctta ccaagaaca tggaatact cagcgtgtt acttctgag cattctgca gattgactg tctacaaat tattgctag gtcgtcaag atatacttc acatagata cctcaatca aggtgcaca gaacttgtt catcaagat aaaagatcg gtgtaccaa ctctaatca tcagaatgg agcctgtca tgccaactt cagtggtaa ttgtctagt caggaattt aggagctac atttaaata aactcaaaa aaccgcaaa tttttgaag catttaata tggggagtt tgaagtttt gtgagattt tttatttat ttgtttata acttgttta gttttattc ttgtaataa gtaaataga ttatctttg ctacgagaa gtaaacata aaatttact gatttattt gttattttc aaattgtaa atgaaagtg aataaagga cctcactag ta
