Status
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Gene information
General information
TC031231
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001679,TC001678
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007424.3: 2843913..2865157
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
      TC031231
      Gene ontology

      GO terms for Tribolium

      • {{ goTerm.term }}
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        Status: {{ goTerm.status }}
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        Quotation: {{ goTerm.quotation }}
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      There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


      GO terms for the homologous Drosophila genes


      Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


      Gene
      {{ goGene }}
      The Tribolium gene the GO term is assigned to.
      GO ID *
      {{ blurIdResult }}
      The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
      Evidence
      {{ blurEvidenceResult }}
      The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
      Reference *
      {{ blurReferenceResult }}
      The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
      Quotation
      If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
      Name
      You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
      Email
      We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
      Publish name
      If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
        {{ unavailableText }}

      * : mandatory fields
      For help on how to submit larger datasets or non experimental data please contact us.
      iBeetle screen iB_03230

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 30.0% (includes death as pupa, adult)

      eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
      Days post injection


      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      iBeetle screen iB_03231

      Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

      iB sequence
      Phenotype after pupal injection
      Usually 10 injected animals

      Pupal injection procedure

      Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

      3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

      9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

      11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

      Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

      13 dpi: Egg productivity and Ovary analysis
      The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

      14 dpi: Analysis of embryonic musculature and early embryonic development
      Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

      15 dpi: Analysis of larval instar 1 cuticle
      Offspring of injected females were analysed and cuticle phenotypes were annotated.

      22 dpi: Stink gland analysis
      Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

      Terms used in the pupal injection procedure

      larval pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      empty eggs
      Just empty shells are visible, no cuticle has been developed.
      strong defects
      L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
      strongly fragmented (cuticle crumbs)
      No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
      cuticle remnants (with unidentifiable segments)
      Segments or parts of appendages are recognizable, but identity is not clear.
      cuticle remnants (with some identifiable segments)
      At least one segment or appendage is clearly regonizable.
      number of eggs on slide
      affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
      For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
      total number of affected eggs/embryos/larvae
      Summary of all different phenotypes/ phenotypic series which were annotated.
      inside-out
      The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      Please see the help page for more information
      Metamorphosis and survival

      Lethality 11 days after pupal injection: 0.0% (includes death as pupa, adult)

      Analysis of larval stage 1 cuticle
      number of eggs/embryos/larvae on slide: < 20
      Phenotype after larval injection
      Usually 10 injected animals

      Larval injection procedure

      Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

      11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

      16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

      19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

      22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

      41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

      Terms used in the larval injection procedure

      pupal/ adult pantagmatic defects
      At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
      pupal molt not fulfilled
      The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
      eclosion not fulfilled
      The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
      starved
      Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
      Metamorphosis and survival

      Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
      Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

      RNAi sequence
      Primers
      Left: Right:
      Protein sequence
      • >TC031231 RA
        MILSRKHVA WSAPTLFRR PFILATDAS GIAISAILS 
        QKYEIEHPI CYASRSLKD AETRYSTIE REALAIVWA 
        VKYFRCYLT GARFTIITD HRPLKYLLT IKEPSSRLA 
        KWAMSLTEF DFEVQYRPG KQHHVDAFT RLEHPQEEM 
        SIQAIQQDC TPIIYNLEE IKKAQSEDK DIQSLLPQE 
        GYYQSPNGL CYRERLDGE RHDKLLVPK TMQRRILQL 
        YHDSPFMAH GGRTKTTEL LKREFYWKG KRHGIRTHG 
        TPYSKGYQN HHENWYPVI QPIADHPWL INLTIATQE 
        PHPLPGSWD VTWRETESK RWGIVVTQD FPIPLAFKC 
        GTEYEIQAV RRSPQSFTT DYTQTILYN QPCPKVPDT 
        SFSNFLKYP VHTVPWILP VLLTFTALV FLLWLLLLG 
        HIIKSSRQG KTHNVQNQN QGPTYHRCS SIAPDPEDP 
        PNETTPTRV DVPYDVPHS PPRAVFTLL PHFTPEKAI 
        EAIDILAKL QPPPKHPHH RCRCVKKLP KIVEEVHYA 
        NIGECKEVS VCKSDRARG FFLLDLRDK IVDLLLGNH 
        NVTPHFRSG CSKIALSGT LLLGVAVRV VVRKKGRHP 
        GVDPTPSSH LVGIKMNFE DVTTKMVDA MTQTTGITI 
        GEERSFADF TELAEREWT EVHYVKVKM TAISPLDMS 
        EEVSPAIII SRDKNDERG LLRSFLLRF NPVLREVMC 
        ESENPLKFE VVSIKQRVT LTEPNESPQ PPKDTYMFV 
        IVGDELEVA NGSLEKTYH SLVELKNRV KLLPVKQIA 
        VACDLRHRP EYHRKMLEY IFRERKEDC KMDTVMISG 
        RAPMITYDS TLREVREHL KDTCSITSV TWINSGKFL 
        FQVEKGLGR IVKEILLSK MGQYWIAWK GPNTQFRRP 
        EKKQDVFCY KCWELGHLG TECKGVDRR HLCQWCGEE 
        GHTRKECTN HYFCPVCNQ NKHRARTPR CGNTQKKAQ 
        KAAECTENT KTIQTEERS FLEFRKLVR SEWSDTVEY 
        EKVKTLAVS PFDMPADVA TAIIISRLQ KEEGALLRS 
        LYERYPELR VEMRECGKV MEYGVKYLR QSKVCVPHE 
        DSYVFVVVA NALKGAVGK LQRTVNALL DLNEEVRRA 
        GIAKIAVAC DQMFKLDYH RKMLEYIFR DEEVEVTFC 
        TPLSGEEVD ISLPKKDKP SHDTIFITG HGRNVTYRS 
        LLLEVREHL KDFESVITE IKKVHRRKL LMQIEKGQG 
        SYISEILRS KMSQHVIYL RAVEQPPYD GFCSKCWEY 
        GHTRKTCQG LNRFYNCRR TGLVWIFKL QDTLRLLPL 
        NLIFEAFFV LYVVPYQEH GILSVLLLS ILQIDCLQI 
        IARSSRYTS HRYLNQGAQ NLFIKIKDR CTNSNHQNG 
        ACHANFSGN CLVRNLGAT FK
      • >TC031231 RB
        MILSRKHVA WSAPTLFRR PFILATDAS GIAISAILS 
        QKYEIEHPI CYASRSLKD AETRYSTIE REALAIVWA 
        VKYFRCYLT GARFTIITD HRPLKYLLT IKEPSSRLA 
        KWAMSLTEF DFEVQYRPG KQHHVDAFT RLEHPQEEM 
        SIQAIQQDC TPIIYNLEE IKKAQSEDK DIQSLLPQE 
        GYYQSPNGL CYRERLDGE RHDKLLVPK TMQRRILQL 
        YHDSPFMAH GGRTKTTEL LKREFYWKV IQPIADHPW 
        LINLTIATQ EPHPLPGSW DVTWRETES KRWGIVVTQ 
        DFPIPLAFK CGTEYEIQA VRRSPQSFT TDYTQTILY 
        NQPCPKVPD TSFSNFLKY PVHTVPWIL PVLLTFTAL 
        VFLLWLLLL GHIIKSSRQ GKTHNVQNQ NQGPTYHRC 
        SSIAPDPED PPNETTPTR VDVPYDVPH SPPRAVFTL 
        LPHFTPEKA IEAIDILAK LQPPPKHPH HRCRCVKKL 
        PKIVEEVHY ANIGECKEV SVCKSDRAR GFFLLDLRD 
        KIVDLLLGN HNVTPHFRS GCSKIALSG TLLLGVAVR 
        VVVRKKGRH PGVDPTPSS HLVGIKMNF EDVTTKMVD 
        AMTQTTGIT IGEERSFAD FTELAEREW TEVHYVKVK 
        MTAISPLDM SEEVSPAII ISRDKNDER GLLRSFLLR 
        FNPVLREVM CESENPLKF EVVSIKQRV TLTEPNESP 
        QPPKDTYMF VIVGDELEV ANGSLEKTY HSLVELKNR 
        VKLLPVKQI AVACDLRHR PEYHRKMLE YIFRERKED 
        CKMDTVMIS GRAPMITYD STLREVREH LKDTCSITS 
        VTWINSGKF LFQVEKGLG RIVKEILLS KMGQYWIAW 
        KGPNTQFRR PEKKQDVFC YKCWELGHL GTECKGVDR 
        RHLCQWCGE EGHTRKECT NHYFCPVCN QNKHRARTP 
        RCGNTQKKA QKAAECTEN TKTIQTEER SFLEFRKLV 
        RSEWSDTVE YEKVKTLAV SPFDMPADV ATAIIISRL 
        QKEEGALLR SLYERYPEL RVEMRECGK VMEYGVKYL 
        RQSKVCVPH EDSYVFVVV ANALKGAVG KLQRTVNAL 
        LDLNEEVRR AGIAKIAVA CDQMFKLDY HRKMLEYIF 
        RDEEVEVTF CTPLSGEEV DISLPKKDK PSHDTIFIT 
        GHGRNVTYR SLLLEVREH LKDFESVIT EIKKVHRRK 
        LLMQIEKGQ GSYISEILR SKMSQHVIY LRAVEQPPY 
        DGFCSKCWE YGHTRKTCQ GLNRFYNCR RTGLVWIFK 
        LQDTLRLLP LNLIFEAFF VLYVVPYQE HGILSVLLL 
        SILQIDCLQ IIARSSRYT SHRYLNQGA QNLFIKIKD 
        RCTNSNHQN GACHANFSG NCLVRNLGA TFK
      CDS sequence
      • >TC031231 RA
        atgatatta tcaagaaag cacgtcgct tggtcagct 
        ccgactttg tttcgacgg cctttcatt ttagcaaca 
        gatgcgtcg ggaattgct atcagtgca attttatcg 
        caaaaatac gaaatcgaa catccaatt tgttatgca 
        agcagaagc ttgaaggac gcggagacc aggtacagc 
        acgattgaa cgggaggcg ttggccatt gtttgggct 
        gtcaaatac tttcgttgt tatttgaca ggtgctcga 
        tttactata attacggac catcgacct ttaaaatac 
        ctcttaaca ataaaagaa cctagttca cgcttagct 
        aaatgggct atgtcctta accgagttc gactttgaa 
        gttcaatac cgtcccgga aaacaacat catgttgat 
        gcttttacc cgattagaa cacccgcaa gaggaaatg 
        tcgattcaa gccatacaa caggattgt actccaatt 
        atctataat ctggaggag atcaagaaa gcccaaagc 
        gaagataag gacattcaa tccttgtta ccacaagag 
        ggatactac caatctcct aatggatta tgctacagg 
        gaacggctg gatggagag cgacatgat aaattattg 
        gtgcccaaa acaatgcaa agaagaatc cttcaattg 
        taccatgat tctcctttt atggcccat ggtggacgc 
        accaaaaca actgaatta ctcaagcga gagttttat 
        tggaagggg aagcgtcat ggaattaga acccatggg 
        acaccatac tcaaagggt taccaaaac caccatgag 
        aattggtat ccagtgatc caacctatc gcggaccac 
        ccttggtta atcaactta accattgcc acacaagag 
        ccacaccct ttgccaggc tcatgggat gttacttgg 
        agggaaact gaatctaag agatggggc attgtggtt 
        acgcaagat ttccccata ccactcgct ttcaaatgt 
        ggaaccgaa tacgagatc caggcagtg agaagaagt 
        cctcaatcc tttacaacg gattatacg caaactatt 
        ctctataat caaccctgt ccaaaggtt ccagacacc 
        tcattcagt aacttcttg aaataccct gttcataca 
        gtgccgtgg attcttcca gttcttctc acgtttact 
        gcattagtg ttcttgcta tggttgctt ttattgggc 
        cacataatc aaaagctca cgacaggga aagacacac 
        aatgttcaa aaccaaaat cagggtcct acgtatcat 
        cgttgctcc agcatagca cctgaccca gaggaccca 
        ccaaacgaa acaacccca accagagtg gatgtacca 
        tacgatgta ccgcattct ccaccaaga gctgtgttc 
        actctgcta ccccacttc actccagag aaagcgata 
        gaagccatt gatatatta gccaaacta cagcctcca 
        ccaaaacac cctcaccac cgttgccga tgtgtaaaa 
        aaactaccc aaaattgtt gaagaagtt cactacgca 
        aacattggt gagtgcaaa gaagtgagt gtgtgtaaa 
        agtgaccgc gcccggggc ttttttctt ttggatttg 
        cgagacaaa attgtcgac ctcctcctc ggtaatcac 
        aacgtcact ccccacttc cgcagtggt tgttcgaaa 
        atcgctttg agtggcacg ttgctcctc ggtgttgcc 
        gtcagggtc gtcgtccgg aagaagggg cgtcaccct 
        ggggtggac cccacacca tcgtcccat ctcgtggga 
        ataaaaatg aattttgaa gatgttaca acgaaaatg 
        gttgatgcc atgacacaa acgacggga ataaccata 
        ggagaagag aggtccttt gccgatttt accgaattg 
        gccgaacgt gaatggacg gaggtccat tacgtgaaa 
        gttaaaatg acggcaatt agtccgctt gatatgtca 
        gaagaggta tctccggct attatcata tctagggac 
        aaaaatgac gaaagggga ttgctgcgc tcgttttta 
        cttcgattt aatccggtt ttgagagag gttatgtgc 
        gaaagtgag aacccctta aagtttgaa gtcgtatcg 
        ataaagcaa agagtaacg ttgacggaa cccaacgaa 
        agtccccaa cctccaaaa gacacgtat atgtttgtg 
        atagtcggt gatgagcta gaagtggca aatggatca 
        ctggaaaaa acataccac tctctagtc gagctcaaa 
        aacagagta aagctattg ccagtcaaa caaatcgct 
        gtggcttgc gatcttaga cacagacct gaataccac 
        cggaagatg ctggagtac atcttcaga gagcgaaaa 
        gaggactgt aaaatggat actgtcatg ataagtggc 
        cgcgcaccg atgattact tacgattcc acgttgcgt 
        gaagtacgt gaacacttg aaggacact tgcagtatc 
        acttctgta acgtggata aattcaggc aaattcttg 
        ttccaagtg gagaaaggg ctaggaagg attgttaag 
        gagatcctc ctctctaaa atgggccaa tattggatc 
        gcatggaag ggtcctaat acacaattc cgacgtccc 
        gaaaagaag caagacgtt ttctgttac aaatgttgg 
        gagttgggt catttgggc acggagtgt aagggtgtc 
        gatagaaga catttgtgt cagtggtgt ggcgaggaa 
        ggccatacc aggaaagag tgcacaaat cactacttc 
        tgtccagtg tgcaaccaa aacaaacac agggcgagg 
        acaccaaga tgtggaaac acccaaaag aaggcacag 
        aaagccgcc gaatgtacg gaaaataca aaaacgatt 
        cagacagaa gagcgatcc tttcttgaa tttaggaag 
        ttggtcagg agtgagtgg agcgatacc gttgagtac 
        gagaaagtt aaaacgctc gctgtaagt ccgtttgac 
        atgcctgca gacgtagcc acggccata atcatatcg 
        cgactgcaa aaggaggaa ggagctttg ttacgctcg 
        ttgtacgag cgttatcca gaattgaga gtggaaatg 
        cgtgaatgc ggcaaggta atggagtat ggagttaag 
        tacttgagg caaagcaaa gtgtgtgta ccacatgag 
        gacagctac gtgtttgtg gtagtagca aacgcattg 
        aaaggggca gtcggtaaa ttacaaaga acagttaat 
        gctctactg gacctgaat gaggaagtt agaagagcg 
        ggaatcgct aaaatcgct gtggcttgc gaccagatg 
        ttcaaactc gattatcac aggaagatg cttgaatat 
        atcttccga gacgaggaa gtggaagtc actttttgc 
        actcccctc tctggcgaa gaagttgac atttcgctt 
        cctaagaaa gataaaccg agtcatgat accatcttt 
        atcactggt catgggagg aatgttaca tacagatcg 
        ctcttactt gaagtgcgt gaacacttg aaagacttt 
        gaatccgtc atcaccgaa ataaagaaa gttcacaga 
        aggaaactc ttaatgcag attgagaaa gggcaaggt 
        tcgtacatt tcggagatc cttcgctca aaaatgagc 
        caacatgtc atctattta agagcggtg gagcaaccg 
        ccgtacgat ggtttctgt tccaagtgt tgggaatat 
        ggccatacg cgtaagact tgccaaggt ttgaacaga 
        ttttataat tgccgcaga acgggtctt gtgtggata 
        tttaagctg caggatact ttgagatta cttcctttg 
        aatttaatt tttgaagct ttcttcgtt ctgtatgta 
        gttccttac caagaacat ggaatactc agcgtgtta 
        cttctgagc attctgcag attgactgt ctacaaatt 
        attgctagg tcgtcaaga tatacttca catagatac 
        ctcaatcaa ggtgcacag aacttgttc atcaagata 
        aaagatcgg tgtaccaac tctaatcat cagaatgga 
        gcctgtcat gccaacttc agtggtaat tgtctagtc 
        aggaattta ggagctaca tttaaataa
      • >TC031231 RB
        atgatatta tcaagaaag cacgtcgct tggtcagct 
        ccgactttg tttcgacgg cctttcatt ttagcaaca 
        gatgcgtcg ggaattgct atcagtgca attttatcg 
        caaaaatac gaaatcgaa catccaatt tgttatgca 
        agcagaagc ttgaaggac gcggagacc aggtacagc 
        acgattgaa cgggaggcg ttggccatt gtttgggct 
        gtcaaatac tttcgttgt tatttgaca ggtgctcga 
        tttactata attacggac catcgacct ttaaaatac 
        ctcttaaca ataaaagaa cctagttca cgcttagct 
        aaatgggct atgtcctta accgagttc gactttgaa 
        gttcaatac cgtcccgga aaacaacat catgttgat 
        gcttttacc cgattagaa cacccgcaa gaggaaatg 
        tcgattcaa gccatacaa caggattgt actccaatt 
        atctataat ctggaggag atcaagaaa gcccaaagc 
        gaagataag gacattcaa tccttgtta ccacaagag 
        ggatactac caatctcct aatggatta tgctacagg 
        gaacggctg gatggagag cgacatgat aaattattg 
        gtgcccaaa acaatgcaa agaagaatc cttcaattg 
        taccatgat tctcctttt atggcccat ggtggacgc 
        accaaaaca actgaatta ctcaagcga gagttttat 
        tggaaggtg atccaacct atcgcggac cacccttgg 
        ttaatcaac ttaaccatt gccacacaa gagccacac 
        cctttgcca ggctcatgg gatgttact tggagggaa 
        actgaatct aagagatgg ggcattgtg gttacgcaa 
        gatttcccc ataccactc gctttcaaa tgtggaacc 
        gaatacgag atccaggca gtgagaaga agtcctcaa 
        tcctttaca acggattat acgcaaact attctctat 
        aatcaaccc tgtccaaag gttccagac acctcattc 
        agtaacttc ttgaaatac cctgttcat acagtgccg 
        tggattctt ccagttctt ctcacgttt actgcatta 
        gtgttcttg ctatggttg cttttattg ggccacata 
        atcaaaagc tcacgacag ggaaagaca cacaatgtt 
        caaaaccaa aatcagggt cctacgtat catcgttgc 
        tccagcata gcacctgac ccagaggac ccaccaaac 
        gaaacaacc ccaaccaga gtggatgta ccatacgat 
        gtaccgcat tctccacca agagctgtg ttcactctg 
        ctaccccac ttcactcca gagaaagcg atagaagcc 
        attgatata ttagccaaa ctacagcct ccaccaaaa 
        caccctcac caccgttgc cgatgtgta aaaaaacta 
        cccaaaatt gttgaagaa gttcactac gcaaacatt 
        ggtgagtgc aaagaagtg agtgtgtgt aaaagtgac 
        cgcgcccgg ggctttttt cttttggat ttgcgagac 
        aaaattgtc gacctcctc ctcggtaat cacaacgtc 
        actccccac ttccgcagt ggttgttcg aaaatcgct 
        ttgagtggc acgttgctc ctcggtgtt gccgtcagg 
        gtcgtcgtc cggaagaag gggcgtcac cctggggtg 
        gaccccaca ccatcgtcc catctcgtg ggaataaaa 
        atgaatttt gaagatgtt acaacgaaa atggttgat 
        gccatgaca caaacgacg ggaataacc ataggagaa 
        gagaggtcc tttgccgat tttaccgaa ttggccgaa 
        cgtgaatgg acggaggtc cattacgtg aaagttaaa 
        atgacggca attagtccg cttgatatg tcagaagag 
        gtatctccg gctattatc atatctagg gacaaaaat 
        gacgaaagg ggattgctg cgctcgttt ttacttcga 
        tttaatccg gttttgaga gaggttatg tgcgaaagt 
        gagaacccc ttaaagttt gaagtcgta tcgataaag 
        caaagagta acgttgacg gaacccaac gaaagtccc 
        caacctcca aaagacacg tatatgttt gtgatagtc 
        ggtgatgag ctagaagtg gcaaatgga tcactggaa 
        aaaacatac cactctcta gtcgagctc aaaaacaga 
        gtaaagcta ttgccagtc aaacaaatc gctgtggct 
        tgcgatctt agacacaga cctgaatac caccggaag 
        atgctggag tacatcttc agagagcga aaagaggac 
        tgtaaaatg gatactgtc atgataagt ggccgcgca 
        ccgatgatt acttacgat tccacgttg cgtgaagta 
        cgtgaacac ttgaaggac acttgcagt atcacttct 
        gtaacgtgg ataaattca ggcaaattc ttgttccaa 
        gtggagaaa gggctagga aggattgtt aaggagatc 
        ctcctctct aaaatgggc caatattgg atcgcatgg 
        aagggtcct aatacacaa ttccgacgt cccgaaaag 
        aagcaagac gttttctgt tacaaatgt tgggagttg 
        ggtcatttg ggcacggag tgtaagggt gtcgataga 
        agacatttg tgtcagtgg tgtggcgag gaaggccat 
        accaggaaa gagtgcaca aatcactac ttctgtcca 
        gtgtgcaac caaaacaaa cacagggcg aggacacca 
        agatgtgga aacacccaa aagaaggca cagaaagcc 
        gccgaatgt acggaaaat acaaaaacg attcagaca 
        gaagagcga tcctttctt gaatttagg aagttggtc 
        aggagtgag tggagcgat accgttgag tacgagaaa 
        gttaaaacg ctcgctgta agtccgttt gacatgcct 
        gcagacgta gccacggcc ataatcata tcgcgactg 
        caaaaggag gaaggagct ttgttacgc tcgttgtac 
        gagcgttat ccagaattg agagtggaa atgcgtgaa 
        tgcggcaag gtaatggag tatggagtt aagtacttg 
        aggcaaagc aaagtgtgt gtaccacat gaggacagc 
        tacgtgttt gtggtagta gcaaacgca ttgaaaggg 
        gcagtcggt aaattacaa agaacagtt aatgctcta 
        ctggacctg aatgaggaa gttagaaga gcgggaatc 
        gctaaaatc gctgtggct tgcgaccag atgttcaaa 
        ctcgattat cacaggaag atgcttgaa tatatcttc 
        cgagacgag gaagtggaa gtcactttt tgcactccc 
        ctctctggc gaagaagtt gacatttcg cttcctaag 
        aaagataaa ccgagtcat gataccatc tttatcact 
        ggtcatggg aggaatgtt acatacaga tcgctctta 
        cttgaagtg cgtgaacac ttgaaagac tttgaatcc 
        gtcatcacc gaaataaag aaagttcac agaaggaaa 
        ctcttaatg cagattgag aaagggcaa ggttcgtac 
        atttcggag atccttcgc tcaaaaatg agccaacat 
        gtcatctat ttaagagcg gtggagcaa ccgccgtac 
        gatggtttc tgttccaag tgttgggaa tatggccat 
        acgcgtaag acttgccaa ggtttgaac agattttat 
        aattgccgc agaacgggt cttgtgtgg atatttaag 
        ctgcaggat actttgaga ttacttcct ttgaattta 
        atttttgaa gctttcttc gttctgtat gtagttcct 
        taccaagaa catggaata ctcagcgtg ttacttctg 
        agcattctg cagattgac tgtctacaa attattgct 
        aggtcgtca agatatact tcacataga tacctcaat 
        caaggtgca cagaacttg ttcatcaag ataaaagat 
        cggtgtacc aactctaat catcagaat ggagcctgt 
        catgccaac ttcagtggt aattgtcta gtcaggaat 
        ttaggagct acatttaaa taa
      mRNA sequence
      • >TC031231 RA
        taaggaatc caaaacttg ctggacaat gatattatc 
        aagaaagca cgtcgcttg gtcagctcc gactttgtt 
        tcgacggcc tttcatttt agcaacaga tgcgtcggg 
        aattgctat cagtgcaat tttatcgca aaaatacga 
        aatcgaaca tccaatttg ttatgcaag cagaagctt 
        gaaggacgc ggagaccag gtacagcac gattgaacg 
        ggaggcgtt ggccattgt ttgggctgt caaatactt 
        tcgttgtta tttgacagg tgctcgatt tactataat 
        tacggacca tcgaccttt aaaatacct cttaacaat 
        aaaagaacc tagttcacg cttagctaa atgggctat 
        gtccttaac cgagttcga ctttgaagt tcaataccg 
        tcccggaaa acaacatca tgttgatgc ttttacccg 
        attagaaca cccgcaaga ggaaatgtc gattcaagc 
        catacaaca ggattgtac tccaattat ctataatct 
        ggaggagat caagaaagc ccaaagcga agataagga 
        cattcaatc cttgttacc acaagaggg atactacca 
        atctcctaa tggattatg ctacaggga acggctgga 
        tggagagcg acatgataa attattggt gcccaaaac 
        aatgcaaag aagaatcct tcaattgta ccatgattc 
        tccttttat ggcccatgg tggacgcac caaaacaac 
        tgaattact caagcgaga gttttattg gaaggggaa 
        gcgtcatgg aattagaac ccatgggac accatactc 
        aaagggtta ccaaaacca ccatgagaa ttggtatcc 
        agtgatcca acctatcgc ggaccaccc ttggttaat 
        caacttaac cattgccac acaagagcc acacccttt 
        gccaggctc atgggatgt tacttggag ggaaactga 
        atctaagag atggggcat tgtggttac gcaagattt 
        ccccatacc actcgcttt caaatgtgg aaccgaata 
        cgagatcca ggcagtgag aagaagtcc tcaatcctt 
        tacaacgga ttatacgca aactattct ctataatca 
        accctgtcc aaaggttcc agacacctc attcagtaa 
        cttcttgaa ataccctgt tcatacagt gccgtggat 
        tcttccagt tcttctcac gtttactgc attagtgtt 
        cttgctatg gttgctttt attgggcca cataatcaa 
        aagctcacg acagggaaa gacacacaa tgttcaaaa 
        ccaaaatca gggtcctac gtatcatcg ttgctccag 
        catagcacc tgacccaga ggacccacc aaacgaaac 
        aaccccaac cagagtgga tgtaccata cgatgtacc 
        gcattctcc accaagagc tgtgttcac tctgctacc 
        ccacttcac tccagagaa agcgataga agccattga 
        tatattagc caaactaca gcctccacc aaaacaccc 
        tcaccaccg ttgccgatg tgtaaaaaa actacccaa 
        aattgttga agaagttca ctacgcaaa cattggtga 
        gtgcaaaga agtgagtgt gtgtaaaag tgaccgcgc 
        ccggggctt ttttctttt ggatttgcg agacaaaat 
        tgtcgacct cctcctcgg taatcacaa cgtcactcc 
        ccacttccg cagtggttg ttcgaaaat cgctttgag 
        tggcacgtt gctcctcgg tgttgccgt cagggtcgt 
        cgtccggaa gaaggggcg tcaccctgg ggtggaccc 
        cacaccatc gtcccatct cgtgggaat aaaaatgaa 
        ttttgaaga tgttacaac gaaaatggt tgatgccat 
        gacacaaac gacgggaat aaccatagg agaagagag 
        gtcctttgc cgattttac cgaattggc cgaacgtga 
        atggacgga ggtccatta cgtgaaagt taaaatgac 
        ggcaattag tccgcttga tatgtcaga agaggtatc 
        tccggctat tatcatatc tagggacaa aaatgacga 
        aaggggatt gctgcgctc gtttttact tcgatttaa 
        tccggtttt gagagaggt tatgtgcga aagtgagaa 
        ccccttaaa gtttgaagt cgtatcgat aaagcaaag 
        agtaacgtt gacggaacc caacgaaag tccccaacc 
        tccaaaaga cacgtatat gtttgtgat agtcggtga 
        tgagctaga agtggcaaa tggatcact ggaaaaaac 
        ataccactc tctagtcga gctcaaaaa cagagtaaa 
        gctattgcc agtcaaaca aatcgctgt ggcttgcga 
        tcttagaca cagacctga ataccaccg gaagatgct 
        ggagtacat cttcagaga gcgaaaaga ggactgtaa 
        aatggatac tgtcatgat aagtggccg cgcaccgat 
        gattactta cgattccac gttgcgtga agtacgtga 
        acacttgaa ggacacttg cagtatcac ttctgtaac 
        gtggataaa ttcaggcaa attcttgtt ccaagtgga 
        gaaagggct aggaaggat tgttaagga gatcctcct 
        ctctaaaat gggccaata ttggatcgc atggaaggg 
        tcctaatac acaattccg acgtcccga aaagaagca 
        agacgtttt ctgttacaa atgttggga gttgggtca 
        tttgggcac ggagtgtaa gggtgtcga tagaagaca 
        tttgtgtca gtggtgtgg cgaggaagg ccataccag 
        gaaagagtg cacaaatca ctacttctg tccagtgtg 
        caaccaaaa caaacacag ggcgaggac accaagatg 
        tggaaacac ccaaaagaa ggcacagaa agccgccga 
        atgtacgga aaatacaaa aacgattca gacagaaga 
        gcgatcctt tcttgaatt taggaagtt ggtcaggag 
        tgagtggag cgataccgt tgagtacga gaaagttaa 
        aacgctcgc tgtaagtcc gtttgacat gcctgcaga 
        cgtagccac ggccataat catatcgcg actgcaaaa 
        ggaggaagg agctttgtt acgctcgtt gtacgagcg 
        ttatccaga attgagagt ggaaatgcg tgaatgcgg 
        caaggtaat ggagtatgg agttaagta cttgaggca 
        aagcaaagt gtgtgtacc acatgagga cagctacgt 
        gtttgtggt agtagcaaa cgcattgaa aggggcagt 
        cggtaaatt acaaagaac agttaatgc tctactgga 
        cctgaatga ggaagttag aagagcggg aatcgctaa 
        aatcgctgt ggcttgcga ccagatgtt caaactcga 
        ttatcacag gaagatgct tgaatatat cttccgaga 
        cgaggaagt ggaagtcac tttttgcac tcccctctc 
        tggcgaaga agttgacat ttcgcttcc taagaaaga 
        taaaccgag tcatgatac catctttat cactggtca 
        tgggaggaa tgttacata cagatcgct cttacttga 
        agtgcgtga acacttgaa agactttga atccgtcat 
        caccgaaat aaagaaagt tcacagaag gaaactctt 
        aatgcagat tgagaaagg gcaaggttc gtacatttc 
        ggagatcct tcgctcaaa aatgagcca acatgtcat 
        ctatttaag agcggtgga gcaaccgcc gtacgatgg 
        tttctgttc caagtgttg ggaatatgg ccatacgcg 
        taagacttg ccaaggttt gaacagatt ttataattg 
        ccgcagaac gggtcttgt gtggatatt taagctgca 
        ggatacttt gagattact tcctttgaa tttaatttt 
        tgaagcttt cttcgttct gtatgtagt tccttacca 
        agaacatgg aatactcag cgtgttact tctgagcat 
        tctgcagat tgactgtct acaaattat tgctaggtc 
        gtcaagata tacttcaca tagatacct caatcaagg 
        tgcacagaa cttgttcat caagataaa agatcggtg 
        taccaactc taatcatca gaatggagc ctgtcatgc 
        caacttcag tggtaattg tctagtcag gaatttagg 
        agctacatt taaataaac tcaaaaaac cgcaaattt 
        ttgaagcat ttaatatgg ggagtttga agttttgtg 
        agatttttt atttatttg tttataact tgtttagtt 
        ttattcttg taataagta aatagatta tctttgcta 
        cgagaagta aacataaaa tttactgat ttatttgtt 
        attttcaaa ttgtaaatg aaagtgaat aaaggacct 
        cactagta
      • >TC031231 RB
        taaggaatc caaaacttg ctggacaat gatattatc 
        aagaaagca cgtcgcttg gtcagctcc gactttgtt 
        tcgacggcc tttcatttt agcaacaga tgcgtcggg 
        aattgctat cagtgcaat tttatcgca aaaatacga 
        aatcgaaca tccaatttg ttatgcaag cagaagctt 
        gaaggacgc ggagaccag gtacagcac gattgaacg 
        ggaggcgtt ggccattgt ttgggctgt caaatactt 
        tcgttgtta tttgacagg tgctcgatt tactataat 
        tacggacca tcgaccttt aaaatacct cttaacaat 
        aaaagaacc tagttcacg cttagctaa atgggctat 
        gtccttaac cgagttcga ctttgaagt tcaataccg 
        tcccggaaa acaacatca tgttgatgc ttttacccg 
        attagaaca cccgcaaga ggaaatgtc gattcaagc 
        catacaaca ggattgtac tccaattat ctataatct 
        ggaggagat caagaaagc ccaaagcga agataagga 
        cattcaatc cttgttacc acaagaggg atactacca 
        atctcctaa tggattatg ctacaggga acggctgga 
        tggagagcg acatgataa attattggt gcccaaaac 
        aatgcaaag aagaatcct tcaattgta ccatgattc 
        tccttttat ggcccatgg tggacgcac caaaacaac 
        tgaattact caagcgaga gttttattg gaaggtgat 
        ccaacctat cgcggacca cccttggtt aatcaactt 
        aaccattgc cacacaaga gccacaccc tttgccagg 
        ctcatggga tgttacttg gagggaaac tgaatctaa 
        gagatgggg cattgtggt tacgcaaga tttccccat 
        accactcgc tttcaaatg tggaaccga atacgagat 
        ccaggcagt gagaagaag tcctcaatc ctttacaac 
        ggattatac gcaaactat tctctataa tcaaccctg 
        tccaaaggt tccagacac ctcattcag taacttctt 
        gaaataccc tgttcatac agtgccgtg gattcttcc 
        agttcttct cacgtttac tgcattagt gttcttgct 
        atggttgct tttattggg ccacataat caaaagctc 
        acgacaggg aaagacaca caatgttca aaaccaaaa 
        tcagggtcc tacgtatca tcgttgctc cagcatagc 
        acctgaccc agaggaccc accaaacga aacaacccc 
        aaccagagt ggatgtacc atacgatgt accgcattc 
        tccaccaag agctgtgtt cactctgct accccactt 
        cactccaga gaaagcgat agaagccat tgatatatt 
        agccaaact acagcctcc accaaaaca ccctcacca 
        ccgttgccg atgtgtaaa aaaactacc caaaattgt 
        tgaagaagt tcactacgc aaacattgg tgagtgcaa 
        agaagtgag tgtgtgtaa aagtgaccg cgcccgggg 
        cttttttct tttggattt gcgagacaa aattgtcga 
        cctcctcct cggtaatca caacgtcac tccccactt 
        ccgcagtgg ttgttcgaa aatcgcttt gagtggcac 
        gttgctcct cggtgttgc cgtcagggt cgtcgtccg 
        gaagaaggg gcgtcaccc tggggtgga ccccacacc 
        atcgtccca tctcgtggg aataaaaat gaattttga 
        agatgttac aacgaaaat ggttgatgc catgacaca 
        aacgacggg aataaccat aggagaaga gaggtcctt 
        tgccgattt taccgaatt ggccgaacg tgaatggac 
        ggaggtcca ttacgtgaa agttaaaat gacggcaat 
        tagtccgct tgatatgtc agaagaggt atctccggc 
        tattatcat atctaggga caaaaatga cgaaagggg 
        attgctgcg ctcgttttt acttcgatt taatccggt 
        tttgagaga ggttatgtg cgaaagtga gaacccctt 
        aaagtttga agtcgtatc gataaagca aagagtaac 
        gttgacgga acccaacga aagtcccca acctccaaa 
        agacacgta tatgtttgt gatagtcgg tgatgagct 
        agaagtggc aaatggatc actggaaaa aacatacca 
        ctctctagt cgagctcaa aaacagagt aaagctatt 
        gccagtcaa acaaatcgc tgtggcttg cgatcttag 
        acacagacc tgaatacca ccggaagat gctggagta 
        catcttcag agagcgaaa agaggactg taaaatgga 
        tactgtcat gataagtgg ccgcgcacc gatgattac 
        ttacgattc cacgttgcg tgaagtacg tgaacactt 
        gaaggacac ttgcagtat cacttctgt aacgtggat 
        aaattcagg caaattctt gttccaagt ggagaaagg 
        gctaggaag gattgttaa ggagatcct cctctctaa 
        aatgggcca atattggat cgcatggaa gggtcctaa 
        tacacaatt ccgacgtcc cgaaaagaa gcaagacgt 
        tttctgtta caaatgttg ggagttggg tcatttggg 
        cacggagtg taagggtgt cgatagaag acatttgtg 
        tcagtggtg tggcgagga aggccatac caggaaaga 
        gtgcacaaa tcactactt ctgtccagt gtgcaacca 
        aaacaaaca cagggcgag gacaccaag atgtggaaa 
        cacccaaaa gaaggcaca gaaagccgc cgaatgtac 
        ggaaaatac aaaaacgat tcagacaga agagcgatc 
        ctttcttga atttaggaa gttggtcag gagtgagtg 
        gagcgatac cgttgagta cgagaaagt taaaacgct 
        cgctgtaag tccgtttga catgcctgc agacgtagc 
        cacggccat aatcatatc gcgactgca aaaggagga 
        aggagcttt gttacgctc gttgtacga gcgttatcc 
        agaattgag agtggaaat gcgtgaatg cggcaaggt 
        aatggagta tggagttaa gtacttgag gcaaagcaa 
        agtgtgtgt accacatga ggacagcta cgtgtttgt 
        ggtagtagc aaacgcatt gaaaggggc agtcggtaa 
        attacaaag aacagttaa tgctctact ggacctgaa 
        tgaggaagt tagaagagc gggaatcgc taaaatcgc 
        tgtggcttg cgaccagat gttcaaact cgattatca 
        caggaagat gcttgaata tatcttccg agacgagga 
        agtggaagt cactttttg cactcccct ctctggcga 
        agaagttga catttcgct tcctaagaa agataaacc 
        gagtcatga taccatctt tatcactgg tcatgggag 
        gaatgttac atacagatc gctcttact tgaagtgcg 
        tgaacactt gaaagactt tgaatccgt catcaccga 
        aataaagaa agttcacag aaggaaact cttaatgca 
        gattgagaa agggcaagg ttcgtacat ttcggagat 
        ccttcgctc aaaaatgag ccaacatgt catctattt 
        aagagcggt ggagcaacc gccgtacga tggtttctg 
        ttccaagtg ttgggaata tggccatac gcgtaagac 
        ttgccaagg tttgaacag attttataa ttgccgcag 
        aacgggtct tgtgtggat atttaagct gcaggatac 
        tttgagatt acttccttt gaatttaat ttttgaagc 
        tttcttcgt tctgtatgt agttcctta ccaagaaca 
        tggaatact cagcgtgtt acttctgag cattctgca 
        gattgactg tctacaaat tattgctag gtcgtcaag 
        atatacttc acatagata cctcaatca aggtgcaca 
        gaacttgtt catcaagat aaaagatcg gtgtaccaa 
        ctctaatca tcagaatgg agcctgtca tgccaactt 
        cagtggtaa ttgtctagt caggaattt aggagctac 
        atttaaata aactcaaaa aaccgcaaa tttttgaag 
        catttaata tggggagtt tgaagtttt gtgagattt 
        tttatttat ttgtttata acttgttta gttttattc 
        ttgtaataa gtaaataga ttatctttg ctacgagaa 
        gtaaacata aaatttact gatttattt gttattttc 
        aaattgtaa atgaaagtg aataaagga cctcactag 
        ta
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