| General information | |||
| TC001677 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007424.3: 2639521..2673671 | |
| Sequence information | |||
| Transcripts / Proteins (4) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC001677 | ||
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03229 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Usually 10 injected animals
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Metamorphosis and survival
Lethality 11 days after pupal injection: 20.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)Days post injection
adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Days post injection
Analysis of larval stage 1 cuticle
number of eggs/embryos/larvae on slide: < 20
Usually 10 injected animals
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Metamorphosis and survival
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001677 RA
MFCCSRLTR LGERMVSMN STLVETVSI NYEDFNESF LTCGTCLCM YDGNEHTPK LLQCSHTVC LHCLTKIAA SQTRDTGSF RCPICRELI TIPRGGVSA LPPSFLVNQ LLDLMSRQR REVIPKCSV HINQELLFC ETCDTVFCT LCTEGSHND TTNSCEHTI IPFSIAIKR MSEILLYKA NECISKLTQ AQEGVSSEL RRLETAKEN CLDQINATF QKVQAFLDN RKQEMIEAL NSTCAEKRR VLEEQHSLI ECEKNKVEQ ECQGLQYQV EVRNITQRI ESLTEKLDA ASNLGEPRE NAFLSCDFT QNNSLEIIQ EQLNILGRV RTSTTFPSL CSATVEGDI IAGIESSIT LNTVDYHGN QRKTGGDPI EAELLPVTP EQNINSVPL EVIDNEDGT YTITFRAPK SGRYGVRIS IFERSIKDS PLFFDVSEH NNPVMAYGC HGSGKDEFI QPVAVVVDE TDQSVYVLD TGNSRIKVL NENLEFVKH ITNEGLLGR SCTGITLSS NGLIVVNWR NKSITEMSK DGETIKTFT YNAFQEPID VAVDKNYGH ILVADNGMN CVFVFDADG KILFQVGKK GTFCQISSV TVGPNSEIL VADYRIQVF SAKGDFMEV IRDEGKGKG RYGGVAIDS EGRILASRS EQKGKNYVQ VLSLTDTNI NNHIDSHES KLKRPSGVA VTAGNHVIV VDLGNNCIK KYRYW
- >TC001677 RB
MDSLQYTIR TRLGERMVS MNSTLVETV SINYEDFNE SFLTCGTCL CMYDGNEHT PKLLQCSHT VCLHCLTKI AASQTRDTG SFRCPICRE LITIPRGGV SALPPSFLV NQLLDLMSR QRREVIPKC SVHINQELL FCETCDTVF CTLCTEGSH NDTTNSCEH TIIPFSIAI KRMSEILLY KANECISKL TQAQEGVSS ELRRLETAK ENCLDQINA TFQKVQAFL DNRKQEMIE ALNSTCAEK RRVLEEQHS LIECEKNKV EQECQGLQY QVEVRNITQ RIESLTEKL DAASNLGEP RENAFLSCD FTQNNSLEI IQEQLNILG RVRTSTTFP SLCSATVEG DIIAGIESS ITLNTVDYH GNQRKTGGD PIEAELLPV TPEQNINSV PLEVIDNED GTYTITFRA PKSGRYGVR ISIFERSIK DSPLFFDVS EHNNPVMAY GCHGSGKDE FIQPVAVVV DETDQSVYV LDTGNSRIK VLNENLEFV KHITNEGLL GRSCTGITL SSNGLIVVN WRNKSITEM SKDGETIKT FTYNAFQEP IDVAVDKNY GHILVADNG MNCVFVFDA DGKILFQVG KKGTFCQIS SVTVGPNSE ILVADYRIQ VFSAKGDFM EVIRDEGKG KGRYGGVAI DSEGRILAS RSEQKGKNY VQVLSLTDT NINNHIDSH ESKLKRPSG VAVTAGNHV IVVDLGNNC IKKYRYW
- >TC001677 RC
MNSTLVETV SINYEDFNE SFLTCGTCL CMYDGNEHT PKLLQCSHT VCLHCLTKI AASQTRDTG SFRCPICRE LITIPRGGV SALPPSFLV NQLLDLMSR QRREVIPKC SVHINQELL FCETCDTVF CTLCTEGSH NDTTNSCEH TIIPFSIAI KRMSEILLY KANECISKL TQAQEGVSS ELRRLETAK ENCLDQINA TFQKVQAFL DNRKQEMIE ALNSTCAEK RRVLEEQHS LIECEKNKV EQECQGLQY QVEVRNITQ RIESLTEKL DAASNLGEP RENAFLSCD FTQNNSLEI IQEQLNILG RVRTSTTFP SLCSATVEG DIIAGIESS ITLNTVDYH GNQRKTGGD PIEAELLPV TPEQNINSV PLEVIDNED GTYTITFRA PKSGRYGVR ISIFERSIK DSPLFFDVS EHNNPVMAY GCHGSGKDE FIQPVAVVV DETDQSVYV LDTGNSRIK VLNENLEFV KHITNEGLL GRSCTGITL SSNGLIVVN WRNKSITEM SKDGETIKT FTYNAFQEP IDVAVDKNY GHILVADNG MNCVFVFDA DGKILFQVG KKGTFCQIS SVTVGPNSE ILVADYRIQ VFSAKGDFM EVIRDEGKG KGRYGGVAI DSEGRILAS RSEQKGKNY VQVLSLTDT NINNHIDSH ESKLKRPSG VAVTAGNHV IVVDLGNNC IKKYRYW
- >TC001677 RD
MGCQKRDFV TKRKLFSNL HLLGMDSLQ YTIRTRLGE RMVSMNSTL VETVSINYE DFNESFLTC GTCLCMYDG NEHTPKLLQ CSHTVCLHC LTKIAASQT RDTGSFRCP ICRELITIP RGGVSALPP SFLVNQLLD LMSRQRREV IPKCSVHIN QELLFCETC DTVFCTLCT EGSHNDTTN SCEHTIIPF SIAIKRMSE ILLYKANEC ISKLTQAQE GVSSELRRL ETAKENCLD QINATFQKV QAFLDNRKQ EMIEALNST CAEKRRVLE EQHSLIECE KNKVEQECQ GLQYQVEVR NITQRIESL TEKLDAASN LGEPRENAF LSCDFTQNN SLEIIQEQL NILGRVRTS TTFPSLCSA TVEGDIIAG IESSITLNT VDYHGNQRK TGGDPIEAE LLPVTPEQN INSVPLEVI DNEDGTYTI TFRAPKSGR YGVRISIFE RSIKDSPLF FDVSEHNNP VMAYGCHGS GKDEFIQPV AVVVDETDQ SVYVLDTGN SRIKVLNEN LEFVKHITN EGLLGRSCT GITLSSNGL IVVNWRNKS ITEMSKDGE TIKTFTYNA FQEPIDVAV DKNYGHILV ADNGMNCVF VFDADGKIL FQVGKKGTF CQISSVTVG PNSEILVAD YRIQVFSAK GDFMEVIRD EGKGKGRYG GVAIDSEGR ILASRSEQK GKNYVQVLS LTDTNINNH IDSHESKLK RPSGVAVTA GNHVIVVDL GNNCIKKYR YW
- >TC001677 RA
atgttttgt tgcagtcgg ctaacgagg ctcggggag aggatggtc agtatgaac tcgaccctc gtcgaaacg gtcagcatc aactatgaa gacttcaac gaaagtttc ctcacttgc ggcacatgt ctatgcatg tacgacggc aacgagcac acaccgaaa cttcttcaa tgttcacac actgtttgt cttcactgt ttgacgaaa atcgccgct tcacaaacg agagacacg ggatccttc cgatgcccc atctgcaga gaattgata acaatacca cgagggggc gtttcggct ttaccaccc tcgttttta gtcaaccag ttgctggat ctcatgtcg agacagagg agagaagtg attccgaaa tgctcggtg cacatcaac caggaactg ctcttctgt gagacgtgc gatactgtt ttttgcaca ctctgcact gaaggatct cacaatgat acgaccaat agttgcgaa cacacgatc atccctttt tcgatcgcg atcaagagg atgtcggag attttgttg tataaggcc aacgagtgt atttcaaag ctaacgcaa gcacaagaa ggtgtcagt tcggaatta cgtaggttg gaaactgcc aaagagaat tgtcttgat cagatcaat gcgacattc cagaaagtt caagcgttt ttggacaat aggaaacag gaaatgatc gaggcgctg aattcgacg tgtgcggag aaaaggagg gttttggaa gagcagcat tcgctgatt gagtgcgaa aagaataag gtggaacaa gaatgccaa ggcctccaa taccaagta gaagtccgc aacataacc caacgcatc gaaagcctc accgagaaa ctcgacgcc gcttcaaac ttaggcgaa ccgcgcgaa aacgctttc ctctcatgc gatttcact caaaacaac tctcttgaa ataatccaa gaacaacta aacattcta ggccgcgtt cgcaccagc accaccttc ccgagcctc tgttcggcc acagtagag ggcgatata atcgcaggt atcgaaagt agcatcaca ctcaacacc gttgactac cacggcaat caacgcaaa actggtgga gacccgatc gaggccgaa ctgctcccg gtcacaccc gaacaaaac ataaactcc gtcccgttg gaagtgata gataatgaa gacggcact tatacgata acgtttaga gcgcctaaa tcgggacga tatggtgtt agaatttcg atttttgag cgctcgatc aaagatagt ccgttgttt ttcgacgtt tcggaacat aacaacccc gtcatggct tacggttgc catgggagc ggtaaagat gagttcata cagccggtg gctgttgtg gtcgacgag acggatcag agtgtctac gttctggat acgggcaat tcgcgaatc aaagttttg aacgaaaat cttgagttt gtcaaacac attacgaac gagggattg cttggtcgg agctgtacc ggaataaca ttatcaagc aatggtttg attgttgtc aattggagg aataagagt attacggag atgtccaaa gatggggag actattaag acctttacc tataatgcg ttccaggag ccaattgat gtggcggtt gataaaaat tacgggcat attttggtt gctgataat gggatgaat tgtgtgttt gtgtttgac gcagatggg aagattttg tttcaagtg ggaaagaag ggaacgttt tgtcaaatt tcctcggtt actgttggg cccaatagt gagattctt gtcgctgat tataggatt caagtgttt tcggcgaaa ggagatttt atggaagtg ataagggac gaggggaaa ggcaaagga cgttacgga ggcgtcgcc atcgattcc gagggtcgt attttggcg agtcgatcc gaacaaaag ggtaaaaac tacgttcaa gttttgagt ctcacagac actaacatc aacaatcac attgattcg cacgagagc aaattaaaa agacccagc ggtgtggcg gtcactgca ggcaatcat gtcatagtc gtagattta gggaataac tgtattaaa aaatatcga tattggtga
- >TC001677 RB
atggattcg ttacagtac actattaga acgaggctc ggggagagg atggtcagt atgaactcg accctcgtc gaaacggtc agcatcaac tatgaagac ttcaacgaa agtttcctc acttgcggc acatgtcta tgcatgtac gacggcaac gagcacaca ccgaaactt cttcaatgt tcacacact gtttgtctt cactgtttg acgaaaatc gccgcttca caaacgaga gacacggga tccttccga tgccccatc tgcagagaa ttgataaca ataccacga gggggcgtt tcggcttta ccaccctcg tttttagtc aaccagttg ctggatctc atgtcgaga cagaggaga gaagtgatt ccgaaatgc tcggtgcac atcaaccag gaactgctc ttctgtgag acgtgcgat actgttttt tgcacactc tgcactgaa ggatctcac aatgatacg accaatagt tgcgaacac acgatcatc cctttttcg atcgcgatc aagaggatg tcggagatt ttgttgtat aaggccaac gagtgtatt tcaaagcta acgcaagca caagaaggt gtcagttcg gaattacgt aggttggaa actgccaaa gagaattgt cttgatcag atcaatgcg acattccag aaagttcaa gcgtttttg gacaatagg aaacaggaa atgatcgag gcgctgaat tcgacgtgt gcggagaaa aggagggtt ttggaagag cagcattcg ctgattgag tgcgaaaag aataaggtg gaacaagaa tgccaaggc ctccaatac caagtagaa gtccgcaac ataacccaa cgcatcgaa agcctcacc gagaaactc gacgccgct tcaaactta ggcgaaccg cgcgaaaac gctttcctc tcatgcgat ttcactcaa aacaactct cttgaaata atccaagaa caactaaac attctaggc cgcgttcgc accagcacc accttcccg agcctctgt tcggccaca gtagagggc gatataatc gcaggtatc gaaagtagc atcacactc aacaccgtt gactaccac ggcaatcaa cgcaaaact ggtggagac ccgatcgag gccgaactg ctcccggtc acacccgaa caaaacata aactccgtc ccgttggaa gtgatagat aatgaagac ggcacttat acgataacg tttagagcg cctaaatcg ggacgatat ggtgttaga atttcgatt tttgagcgc tcgatcaaa gatagtccg ttgtttttc gacgtttcg gaacataac aaccccgtc atggcttac ggttgccat gggagcggt aaagatgag ttcatacag ccggtggct gttgtggtc gacgagacg gatcagagt gtctacgtt ctggatacg ggcaattcg cgaatcaaa gttttgaac gaaaatctt gagtttgtc aaacacatt acgaacgag ggattgctt ggtcggagc tgtaccgga ataacatta tcaagcaat ggtttgatt gttgtcaat tggaggaat aagagtatt acggagatg tccaaagat ggggagact attaagacc tttacctat aatgcgttc caggagcca attgatgtg gcggttgat aaaaattac gggcatatt ttggttgct gataatggg atgaattgt gtgtttgtg tttgacgca gatgggaag attttgttt caagtggga aagaaggga acgttttgt caaatttcc tcggttact gttgggccc aatagtgag attcttgtc gctgattat aggattcaa gtgttttcg gcgaaagga gattttatg gaagtgata agggacgag gggaaaggc aaaggacgt tacggaggc gtcgccatc gattccgag ggtcgtatt ttggcgagt cgatccgaa caaaagggt aaaaactac gttcaagtt ttgagtctc acagacact aacatcaac aatcacatt gattcgcac gagagcaaa ttaaaaaga cccagcggt gtggcggtc actgcaggc aatcatgtc atagtcgta gatttaggg aataactgt attaaaaaa tatcgatat tggtga
- >TC001677 RC
atgaactcg accctcgtc gaaacggtc agcatcaac tatgaagac ttcaacgaa agtttcctc acttgcggc acatgtcta tgcatgtac gacggcaac gagcacaca ccgaaactt cttcaatgt tcacacact gtttgtctt cactgtttg acgaaaatc gccgcttca caaacgaga gacacggga tccttccga tgccccatc tgcagagaa ttgataaca ataccacga gggggcgtt tcggcttta ccaccctcg tttttagtc aaccagttg ctggatctc atgtcgaga cagaggaga gaagtgatt ccgaaatgc tcggtgcac atcaaccag gaactgctc ttctgtgag acgtgcgat actgttttt tgcacactc tgcactgaa ggatctcac aatgatacg accaatagt tgcgaacac acgatcatc cctttttcg atcgcgatc aagaggatg tcggagatt ttgttgtat aaggccaac gagtgtatt tcaaagcta acgcaagca caagaaggt gtcagttcg gaattacgt aggttggaa actgccaaa gagaattgt cttgatcag atcaatgcg acattccag aaagttcaa gcgtttttg gacaatagg aaacaggaa atgatcgag gcgctgaat tcgacgtgt gcggagaaa aggagggtt ttggaagag cagcattcg ctgattgag tgcgaaaag aataaggtg gaacaagaa tgccaaggc ctccaatac caagtagaa gtccgcaac ataacccaa cgcatcgaa agcctcacc gagaaactc gacgccgct tcaaactta ggcgaaccg cgcgaaaac gctttcctc tcatgcgat ttcactcaa aacaactct cttgaaata atccaagaa caactaaac attctaggc cgcgttcgc accagcacc accttcccg agcctctgt tcggccaca gtagagggc gatataatc gcaggtatc gaaagtagc atcacactc aacaccgtt gactaccac ggcaatcaa cgcaaaact ggtggagac ccgatcgag gccgaactg ctcccggtc acacccgaa caaaacata aactccgtc ccgttggaa gtgatagat aatgaagac ggcacttat acgataacg tttagagcg cctaaatcg ggacgatat ggtgttaga atttcgatt tttgagcgc tcgatcaaa gatagtccg ttgtttttc gacgtttcg gaacataac aaccccgtc atggcttac ggttgccat gggagcggt aaagatgag ttcatacag ccggtggct gttgtggtc gacgagacg gatcagagt gtctacgtt ctggatacg ggcaattcg cgaatcaaa gttttgaac gaaaatctt gagtttgtc aaacacatt acgaacgag ggattgctt ggtcggagc tgtaccgga ataacatta tcaagcaat ggtttgatt gttgtcaat tggaggaat aagagtatt acggagatg tccaaagat ggggagact attaagacc tttacctat aatgcgttc caggagcca attgatgtg gcggttgat aaaaattac gggcatatt ttggttgct gataatggg atgaattgt gtgtttgtg tttgacgca gatgggaag attttgttt caagtggga aagaaggga acgttttgt caaatttcc tcggttact gttgggccc aatagtgag attcttgtc gctgattat aggattcaa gtgttttcg gcgaaagga gattttatg gaagtgata agggacgag gggaaaggc aaaggacgt tacggaggc gtcgccatc gattccgag ggtcgtatt ttggcgagt cgatccgaa caaaagggt aaaaactac gttcaagtt ttgagtctc acagacact aacatcaac aatcacatt gattcgcac gagagcaaa ttaaaaaga cccagcggt gtggcggtc actgcaggc aatcatgtc atagtcgta gatttaggg aataactgt attaaaaaa tatcgatat tggtga
- >TC001677 RD
atggggtgt cagaaacgc gattttgta acgaaacga aaattattt tcgaatttg caccttctc ggaatggat tcgttacag tacactatt agaacgagg ctcggggag aggatggtc agtatgaac tcgaccctc gtcgaaacg gtcagcatc aactatgaa gacttcaac gaaagtttc ctcacttgc ggcacatgt ctatgcatg tacgacggc aacgagcac acaccgaaa cttcttcaa tgttcacac actgtttgt cttcactgt ttgacgaaa atcgccgct tcacaaacg agagacacg ggatccttc cgatgcccc atctgcaga gaattgata acaatacca cgagggggc gtttcggct ttaccaccc tcgttttta gtcaaccag ttgctggat ctcatgtcg agacagagg agagaagtg attccgaaa tgctcggtg cacatcaac caggaactg ctcttctgt gagacgtgc gatactgtt ttttgcaca ctctgcact gaaggatct cacaatgat acgaccaat agttgcgaa cacacgatc atccctttt tcgatcgcg atcaagagg atgtcggag attttgttg tataaggcc aacgagtgt atttcaaag ctaacgcaa gcacaagaa ggtgtcagt tcggaatta cgtaggttg gaaactgcc aaagagaat tgtcttgat cagatcaat gcgacattc cagaaagtt caagcgttt ttggacaat aggaaacag gaaatgatc gaggcgctg aattcgacg tgtgcggag aaaaggagg gttttggaa gagcagcat tcgctgatt gagtgcgaa aagaataag gtggaacaa gaatgccaa ggcctccaa taccaagta gaagtccgc aacataacc caacgcatc gaaagcctc accgagaaa ctcgacgcc gcttcaaac ttaggcgaa ccgcgcgaa aacgctttc ctctcatgc gatttcact caaaacaac tctcttgaa ataatccaa gaacaacta aacattcta ggccgcgtt cgcaccagc accaccttc ccgagcctc tgttcggcc acagtagag ggcgatata atcgcaggt atcgaaagt agcatcaca ctcaacacc gttgactac cacggcaat caacgcaaa actggtgga gacccgatc gaggccgaa ctgctcccg gtcacaccc gaacaaaac ataaactcc gtcccgttg gaagtgata gataatgaa gacggcact tatacgata acgtttaga gcgcctaaa tcgggacga tatggtgtt agaatttcg atttttgag cgctcgatc aaagatagt ccgttgttt ttcgacgtt tcggaacat aacaacccc gtcatggct tacggttgc catgggagc ggtaaagat gagttcata cagccggtg gctgttgtg gtcgacgag acggatcag agtgtctac gttctggat acgggcaat tcgcgaatc aaagttttg aacgaaaat cttgagttt gtcaaacac attacgaac gagggattg cttggtcgg agctgtacc ggaataaca ttatcaagc aatggtttg attgttgtc aattggagg aataagagt attacggag atgtccaaa gatggggag actattaag acctttacc tataatgcg ttccaggag ccaattgat gtggcggtt gataaaaat tacgggcat attttggtt gctgataat gggatgaat tgtgtgttt gtgtttgac gcagatggg aagattttg tttcaagtg ggaaagaag ggaacgttt tgtcaaatt tcctcggtt actgttggg cccaatagt gagattctt gtcgctgat tataggatt caagtgttt tcggcgaaa ggagatttt atggaagtg ataagggac gaggggaaa ggcaaagga cgttacgga ggcgtcgcc atcgattcc gagggtcgt attttggcg agtcgatcc gaacaaaag ggtaaaaac tacgttcaa gttttgagt ctcacagac actaacatc aacaatcac attgattcg cacgagagc aaattaaaa agacccagc ggtgtggcg gtcactgca ggcaatcat gtcatagtc gtagattta gggaataac tgtattaaa aaatatcga tattggtga
- >TC001677 RA
ttagttaga aaataaaac taaaaatac ataaccaga ttttatttt aaactctat aatacaaaa gccaacact tacatacaa aattgtcaa aatcaccta aactttaac acaatatac aacacaaag aagacaaac aatgagaag aaaaataaa tacacgatg taataatta tgagacccc tttgacaac cgcaaaacc ctattcata gtattcccc aaaaacccc ctagtcctg tccatatcg tcatccaca tctcgcaac aatttgtcc tgatatttc agctcgttt cctatatct atcgaaagg gacttcagt gcaccaacc ttgtccctt aattcatta gccattcgg tcattttcc tcctcgact tcgttagaa gtttggggc agaggctcg tagtagttt ccggcatgg gcccttcgc attttgaac gattccagc tttaaggtt atgccaaac tcgtccgga aatttcact tcgggtctt cacccgttg tttcactgt aactttaca atttcacaa tgtttttgc aaatttgtt aacaggttg acgtgttca attcaattt ttgaggtta ctaacgtca agtctaggg aatttgcgt caataccac ccaccagca aataaattg ataaacaca cgtagagga taaataata atggaaatt gaattaaat ttgcgtgag gttaacaat ttgttgcat tctgatttt gtacaagtt ggttggggt cggtaattt caaaattct ggtgttttt cggtggtgg ttgttggtt gtagtttcg cgtcaggca accagtaat acaacaaag cgcacaaga agtaatatt tgtggtgaa aacgtattc acgttgatt cgtgctgtt tatgctagt tcgtgtgaa aaaccgtcg aaataatgt aaattcagc cttttccag ttcgtcgac tccggaagg tgttgtgcg gacatgtga ttgatcggg aatttacac caagtcctg aatgttttg ttgcagtcg gctaacgag gctcgggga gaggatggt cagtatgaa ctcgaccct cgtcgaaac ggtcagcat caactatga agacttcaa cgaaagttt cctcacttg cggcacatg tctatgcat gtacgacgg caacgagca cacaccgaa acttcttca atgttcaca cactgtttg tcttcactg tttgacgaa aatcgccgc ttcacaaac gagagacac gggatcctt ccgatgccc catctgcag agaattgat aacaatacc acgaggggg cgtttcggc tttaccacc ctcgttttt agtcaacca gttgctgga tctcatgtc gagacagag gagagaagt gattccgaa atgctcggt gcacatcaa ccaggaact gctcttctg tgagacgtg cgatactgt tttttgcac actctgcac tgaaggatc tcacaatga tacgaccaa tagttgcga acacacgat catcccttt ttcgatcgc gatcaagag gatgtcgga gattttgtt gtataaggc caacgagtg tatttcaaa gctaacgca agcacaaga aggtgtcag ttcggaatt acgtaggtt ggaaactgc caaagagaa ttgtcttga tcagatcaa tgcgacatt ccagaaagt tcaagcgtt tttggacaa taggaaaca ggaaatgat cgaggcgct gaattcgac gtgtgcgga gaaaaggag ggttttgga agagcagca ttcgctgat tgagtgcga aaagaataa ggtggaaca agaatgcca aggcctcca ataccaagt agaagtccg caacataac ccaacgcat cgaaagcct caccgagaa actcgacgc cgcttcaaa cttaggcga accgcgcga aaacgcttt cctctcatg cgatttcac tcaaaacaa ctctcttga aataatcca agaacaact aaacattct aggccgcgt tcgcaccag caccacctt cccgagcct ctgttcggc cacagtaga gggcgatat aatcgcagg tatcgaaag tagcatcac actcaacac cgttgacta ccacggcaa tcaacgcaa aactggtgg agacccgat cgaggccga actgctccc ggtcacacc cgaacaaaa cataaactc cgtcccgtt ggaagtgat agataatga agacggcac ttatacgat aacgtttag agcgcctaa atcgggacg atatggtgt tagaatttc gatttttga gcgctcgat caaagatag tccgttgtt tttcgacgt ttcggaaca taacaaccc cgtcatggc ttacggttg ccatgggag cggtaaaga tgagttcat acagccggt ggctgttgt ggtcgacga gacggatca gagtgtcta cgttctgga tacgggcaa ttcgcgaat caaagtttt gaacgaaaa tcttgagtt tgtcaaaca cattacgaa cgagggatt gcttggtcg gagctgtac cggaataac attatcaag caatggttt gattgttgt caattggag gaataagag tattacgga gatgtccaa agatgggga gactattaa gacctttac ctataatgc gttccagga gccaattga tgtggcggt tgataaaaa ttacgggca tattttggt tgctgataa tgggatgaa ttgtgtgtt tgtgtttga cgcagatgg gaagatttt gtttcaagt gggaaagaa gggaacgtt ttgtcaaat ttcctcggt tactgttgg gcccaatag tgagattct tgtcgctga ttataggat tcaagtgtt ttcggcgaa aggagattt tatggaagt gataaggga cgaggggaa aggcaaagg acgttacgg aggcgtcgc catcgattc cgagggtcg tattttggc gagtcgatc cgaacaaaa gggtaaaaa ctacgttca agttttgag tctcacaga cactaacat caacaatca cattgattc gcacgagag caaattaaa aagacccag cggtgtggc ggtcactgc aggcaatca tgtcatagt cgtagattt agggaataa ctgtattaa aaaatatcg atattggtg aaaatttta ccatcatta tactctctc tattttttg actgcagtt tgcagatct cagtagtca cggttttga acacatttt gtattttgt tccgtgttg acaggtcag tcattgctt atcgccact aacttatct ttaagcgaa gtgtattca aacgttgca gcgttcgat tagtttgtt agttgcttc atttttttt tctactttt atgataaac ctgaggagc gagtcgaag gaggaaaat gttactgat tgttataaa gtgatgaat ttttacgct gttttgctg aagccgtgc acgaacttg cagacttaa taaattgtc tgtgcttat aatggtttc acccagttt ttgtactta aattaaaaa taatctttt aattttttg tactgtttt tggaagtat acttttgtt ttctagtca ttcattgcg catattttt atactgtgt agtacaatt attttgtac acaataaat ttcttgata catc
- >TC001677 RB
tgtgttgta aacgcaagt tgttttttt atgtagtca gaaacgcga ttttgtaac gaaacgaaa attattttc gaatttgca ccttctcgg aatggattc gttacagta cactattag aacgaggct cggggagag gatggtcag tatgaactc gaccctcgt cgaaacggt cagcatcaa ctatgaaga cttcaacga aagtttcct cacttgcgg cacatgtct atgcatgta cgacggcaa cgagcacac accgaaact tcttcaatg ttcacacac tgtttgtct tcactgttt gacgaaaat cgccgcttc acaaacgag agacacggg atccttccg atgccccat ctgcagaga attgataac aataccacg agggggcgt ttcggcttt accaccctc gtttttagt caaccagtt gctggatct catgtcgag acagaggag agaagtgat tccgaaatg ctcggtgca catcaacca ggaactgct cttctgtga gacgtgcga tactgtttt ttgcacact ctgcactga aggatctca caatgatac gaccaatag ttgcgaaca cacgatcat ccctttttc gatcgcgat caagaggat gtcggagat tttgttgta taaggccaa cgagtgtat ttcaaagct aacgcaagc acaagaagg tgtcagttc ggaattacg taggttgga aactgccaa agagaattg tcttgatca gatcaatgc gacattcca gaaagttca agcgttttt ggacaatag gaaacagga aatgatcga ggcgctgaa ttcgacgtg tgcggagaa aaggagggt tttggaaga gcagcattc gctgattga gtgcgaaaa gaataaggt ggaacaaga atgccaagg cctccaata ccaagtaga agtccgcaa cataaccca acgcatcga aagcctcac cgagaaact cgacgccgc ttcaaactt aggcgaacc gcgcgaaaa cgctttcct ctcatgcga tttcactca aaacaactc tcttgaaat aatccaaga acaactaaa cattctagg ccgcgttcg caccagcac caccttccc gagcctctg ttcggccac agtagaggg cgatataat cgcaggtat cgaaagtag catcacact caacaccgt tgactacca cggcaatca acgcaaaac tggtggaga cccgatcga ggccgaact gctcccggt cacacccga acaaaacat aaactccgt cccgttgga agtgataga taatgaaga cggcactta tacgataac gtttagagc gcctaaatc gggacgata tggtgttag aatttcgat ttttgagcg ctcgatcaa agatagtcc gttgttttt cgacgtttc ggaacataa caaccccgt catggctta cggttgcca tgggagcgg taaagatga gttcataca gccggtggc tgttgtggt cgacgagac ggatcagag tgtctacgt tctggatac gggcaattc gcgaatcaa agttttgaa cgaaaatct tgagtttgt caaacacat tacgaacga gggattgct tggtcggag ctgtaccgg aataacatt atcaagcaa tggtttgat tgttgtcaa ttggaggaa taagagtat tacggagat gtccaaaga tggggagac tattaagac ctttaccta taatgcgtt ccaggagcc aattgatgt ggcggttga taaaaatta cgggcatat tttggttgc tgataatgg gatgaattg tgtgtttgt gtttgacgc agatgggaa gattttgtt tcaagtggg aaagaaggg aacgttttg tcaaatttc ctcggttac tgttgggcc caatagtga gattcttgt cgctgatta taggattca agtgttttc ggcgaaagg agattttat ggaagtgat aagggacga ggggaaagg caaaggacg ttacggagg cgtcgccat cgattccga gggtcgtat tttggcgag tcgatccga acaaaaggg taaaaacta cgttcaagt tttgagtct cacagacac taacatcaa caatcacat tgattcgca cgagagcaa attaaaaag acccagcgg tgtggcggt cactgcagg caatcatgt catagtcgt agatttagg gaataactg tattaaaaa atatcgata ttggtgaaa attttacca tcattatac tctctctat tttttgact gcagtttgc agatctcag tagtcacgg ttttgaaca cattttgta ttttgttcc gtgttgaca ggtcagtca ttgcttatc gccactaac ttatcttta agcgaagtg tattcaaac gttgcagcg ttcgattag tttgttagt tgcttcatt tttttttct acttttatg ataaacctg aggagcgag tcgaaggag gaaaatgtt actgattgt tataaagtg atgaatttt tacgctgtt ttgctgaag ccgtgcacg aacttgcag acttaataa attgtctgt gcttataat ggtttcacc cagtttttg tacttaaat taaaaataa tcttttaat tttttgtac tgtttttgg aagtatact tttgttttc tagtcattc attgcgcat atttttata ctgtgtagt acaattatt ttgtacaca ataaatttc ttgatacat c
- >TC001677 RC
ttagttaga aaataaaac taaaaatac ataaccaga ttttatttt aaactctat aatacaaaa gccaacact tacatacaa aattgtcaa aatcaccta aactttaac acaatatac aacacaaag aagacaaac aatgagaag aaaaataaa tacacgatg taataatta tgagacccc tttgacaac cgcaaaacc ctattcata gtattcccc aaaaacccc ctagtcctg tccatatcg tcatccaca tctcgcaac aatttgtcc tgatatttc agctcgttt cctatatct atcgaaagg gacttcagt gcaccaacc ttgtccctt aattcatta gccattcgg tcattttcc tcctcgact tcgttagaa gtttggggc agaggctcg tagtagttt ccggcatgg gcccttcgc attttgaac gattccagc tttaaggtt atgccaaac tcgtccgga aatttcact tcgggtctt cacccgttg tttcactgt aactttaca atttcacaa tgtttttgc aaatttgtt aacaggttg acgtgttca attcaattt ttgaggtta ctaacgtca agtctaggg aatttgcgt caataccac ccaccagca aataaattg ataaacaca cgtagagga taaataata atggaaatt gaattaaat ttgcgtgag gttaacaat ttgttgcat tctgatttt gtacaagtt ggttggggt cggtaattt caaaattct ggtgttttt cggtggtgg ttgttggtt gtagtttcg cgtcaggca accagtaat acaacaaag cgcacaaga agtaatatt tgtggtgaa aacgtattc acgttgatt cgtgctgtt tatgctagt tcgtgtgaa aaaccgtcg aaataatgt aaattcagc cttttccag aacgaggct cggggagag gatggtcag tatgaactc gaccctcgt cgaaacggt cagcatcaa ctatgaaga cttcaacga aagtttcct cacttgcgg cacatgtct atgcatgta cgacggcaa cgagcacac accgaaact tcttcaatg ttcacacac tgtttgtct tcactgttt gacgaaaat cgccgcttc acaaacgag agacacggg atccttccg atgccccat ctgcagaga attgataac aataccacg agggggcgt ttcggcttt accaccctc gtttttagt caaccagtt gctggatct catgtcgag acagaggag agaagtgat tccgaaatg ctcggtgca catcaacca ggaactgct cttctgtga gacgtgcga tactgtttt ttgcacact ctgcactga aggatctca caatgatac gaccaatag ttgcgaaca cacgatcat ccctttttc gatcgcgat caagaggat gtcggagat tttgttgta taaggccaa cgagtgtat ttcaaagct aacgcaagc acaagaagg tgtcagttc ggaattacg taggttgga aactgccaa agagaattg tcttgatca gatcaatgc gacattcca gaaagttca agcgttttt ggacaatag gaaacagga aatgatcga ggcgctgaa ttcgacgtg tgcggagaa aaggagggt tttggaaga gcagcattc gctgattga gtgcgaaaa gaataaggt ggaacaaga atgccaagg cctccaata ccaagtaga agtccgcaa cataaccca acgcatcga aagcctcac cgagaaact cgacgccgc ttcaaactt aggcgaacc gcgcgaaaa cgctttcct ctcatgcga tttcactca aaacaactc tcttgaaat aatccaaga acaactaaa cattctagg ccgcgttcg caccagcac caccttccc gagcctctg ttcggccac agtagaggg cgatataat cgcaggtat cgaaagtag catcacact caacaccgt tgactacca cggcaatca acgcaaaac tggtggaga cccgatcga ggccgaact gctcccggt cacacccga acaaaacat aaactccgt cccgttgga agtgataga taatgaaga cggcactta tacgataac gtttagagc gcctaaatc gggacgata tggtgttag aatttcgat ttttgagcg ctcgatcaa agatagtcc gttgttttt cgacgtttc ggaacataa caaccccgt catggctta cggttgcca tgggagcgg taaagatga gttcataca gccggtggc tgttgtggt cgacgagac ggatcagag tgtctacgt tctggatac gggcaattc gcgaatcaa agttttgaa cgaaaatct tgagtttgt caaacacat tacgaacga gggattgct tggtcggag ctgtaccgg aataacatt atcaagcaa tggtttgat tgttgtcaa ttggaggaa taagagtat tacggagat gtccaaaga tggggagac tattaagac ctttaccta taatgcgtt ccaggagcc aattgatgt ggcggttga taaaaatta cgggcatat tttggttgc tgataatgg gatgaattg tgtgtttgt gtttgacgc agatgggaa gattttgtt tcaagtggg aaagaaggg aacgttttg tcaaatttc ctcggttac tgttgggcc caatagtga gattcttgt cgctgatta taggattca agtgttttc ggcgaaagg agattttat ggaagtgat aagggacga ggggaaagg caaaggacg ttacggagg cgtcgccat cgattccga gggtcgtat tttggcgag tcgatccga acaaaaggg taaaaacta cgttcaagt tttgagtct cacagacac taacatcaa caatcacat tgattcgca cgagagcaa attaaaaag acccagcgg tgtggcggt cactgcagg caatcatgt catagtcgt agatttagg gaataactg tattaaaaa atatcgata ttggtgaaa attttacca tcattatac tctctctat tttttgact gcagtttgc agatctcag tagtcacgg ttttgaaca cattttgta ttttgttcc gtgttgaca ggtcagtca ttgcttatc gccactaac ttatcttta agcgaagtg tattcaaac gttgcagcg ttcgattag tttgttagt tgcttcatt tttttttct acttttatg ataaacctg aggagcgag tcgaaggag gaaaatgtt actgattgt tataaagtg atgaatttt tacgctgtt ttgctgaag ccgtgcacg aacttgcag acttaataa attgtctgt gcttataat ggtttcacc cagtttttg tacttaaat taaaaataa tcttttaat tttttgtac tgtttttgg aagtatact tttgttttc tagtcattc attgcgcat atttttata ctgtgtagt acaattatt ttgtacaca ataaatttc ttgatacat c
- >TC001677 RD
ttagttaga aaataaaac taaaaatac ataaccaga ttttatttt aaactctat aatacaaaa gccaacact tacatacaa aattgtcaa aatcaccta aactttaac acaatatac aacacaaag aagacaaac aatgagaag aaaaataaa tacacgatg taataatta tgagacccc tttgacaac cgcaaaacc ctattcata gtattcccc aaaaacccc ctagtcctg tccatatcg tcatccaca tctcgcaac aatttgtcc tgatatttc agctcgttt cctatatct atcgaaagg gacttcagt gcaccaacc ttgtccctt aattcatta gccattcgg tcattttcc tcctcgact tcgttagaa gtttggggc agaggctcg tagtagttt ccggcatgg gcccttcgc attttgaac gattccagc tttaaggtt atgccaaac tcgtccgga aatttcact tcgggtctt cacccgttg tttcactgt aactttaca atttcacaa tgtttttgc aaatttgtt aacaggttg acgtgttca attcaattt ttgaggtta ctaacgtca agtctaggg aatttgcgt caataccac ccaccagca aataaattg ataaacaca cgtagagga taaataata atggaaatt gaattaaat ttgcgtgag gttaacaat ttgttgcat tctgatttt gtacaagtt ggttggggt cggtaattt caaaattct ggtgttttt cggtggtgg ttgttggtt gtagtttcg cgtcaggca accagtaat acaacaaag cgcacaaga agtaatatt tgtggtgaa aacgtattc acgttgatt cgtgctgtt tatgctagt tcgtgtgaa aaaccgtcg aaataatgt aaattcagc cttttccag ttcgtcgac tccggaagg tgttgtgcg gacatgtga ttgatcggg aatttacac caagtcctg aatgttttg ttgcagtcg gctgtgagt atggggtgt cagaaacgc gattttgta acgaaacga aaattattt tcgaatttg caccttctc ggaatggat tcgttacag tacactatt agaacgagg ctcggggag aggatggtc agtatgaac tcgaccctc gtcgaaacg gtcagcatc aactatgaa gacttcaac gaaagtttc ctcacttgc ggcacatgt ctatgcatg tacgacggc aacgagcac acaccgaaa cttcttcaa tgttcacac actgtttgt cttcactgt ttgacgaaa atcgccgct tcacaaacg agagacacg ggatccttc cgatgcccc atctgcaga gaattgata acaatacca cgagggggc gtttcggct ttaccaccc tcgttttta gtcaaccag ttgctggat ctcatgtcg agacagagg agagaagtg attccgaaa tgctcggtg cacatcaac caggaactg ctcttctgt gagacgtgc gatactgtt ttttgcaca ctctgcact gaaggatct cacaatgat acgaccaat agttgcgaa cacacgatc atccctttt tcgatcgcg atcaagagg atgtcggag attttgttg tataaggcc aacgagtgt atttcaaag ctaacgcaa gcacaagaa ggtgtcagt tcggaatta cgtaggttg gaaactgcc aaagagaat tgtcttgat cagatcaat gcgacattc cagaaagtt caagcgttt ttggacaat aggaaacag gaaatgatc gaggcgctg aattcgacg tgtgcggag aaaaggagg gttttggaa gagcagcat tcgctgatt gagtgcgaa aagaataag gtggaacaa gaatgccaa ggcctccaa taccaagta gaagtccgc aacataacc caacgcatc gaaagcctc accgagaaa ctcgacgcc gcttcaaac ttaggcgaa ccgcgcgaa aacgctttc ctctcatgc gatttcact caaaacaac tctcttgaa ataatccaa gaacaacta aacattcta ggccgcgtt cgcaccagc accaccttc ccgagcctc tgttcggcc acagtagag ggcgatata atcgcaggt atcgaaagt agcatcaca ctcaacacc gttgactac cacggcaat caacgcaaa actggtgga gacccgatc gaggccgaa ctgctcccg gtcacaccc gaacaaaac ataaactcc gtcccgttg gaagtgata gataatgaa gacggcact tatacgata acgtttaga gcgcctaaa tcgggacga tatggtgtt agaatttcg atttttgag cgctcgatc aaagatagt ccgttgttt ttcgacgtt tcggaacat aacaacccc gtcatggct tacggttgc catgggagc ggtaaagat gagttcata cagccggtg gctgttgtg gtcgacgag acggatcag agtgtctac gttctggat acgggcaat tcgcgaatc aaagttttg aacgaaaat cttgagttt gtcaaacac attacgaac gagggattg cttggtcgg agctgtacc ggaataaca ttatcaagc aatggtttg attgttgtc aattggagg aataagagt attacggag atgtccaaa gatggggag actattaag acctttacc tataatgcg ttccaggag ccaattgat gtggcggtt gataaaaat tacgggcat attttggtt gctgataat gggatgaat tgtgtgttt gtgtttgac gcagatggg aagattttg tttcaagtg ggaaagaag ggaacgttt tgtcaaatt tcctcggtt actgttggg cccaatagt gagattctt gtcgctgat tataggatt caagtgttt tcggcgaaa ggagatttt atggaagtg ataagggac gaggggaaa ggcaaagga cgttacgga ggcgtcgcc atcgattcc gagggtcgt attttggcg agtcgatcc gaacaaaag ggtaaaaac tacgttcaa gttttgagt ctcacagac actaacatc aacaatcac attgattcg cacgagagc aaattaaaa agacccagc ggtgtggcg gtcactgca ggcaatcat gtcatagtc gtagattta gggaataac tgtattaaa aaatatcga tattggtga aaattttac catcattat actctctct attttttga ctgcagttt gcagatctc agtagtcac ggttttgaa cacattttg tattttgtt ccgtgttga caggtcagt cattgctta tcgccacta acttatctt taagcgaag tgtattcaa acgttgcag cgttcgatt agtttgtta gttgcttca ttttttttt ctactttta tgataaacc tgaggagcg agtcgaagg aggaaaatg ttactgatt gttataaag tgatgaatt tttacgctg ttttgctga agccgtgca cgaacttgc agacttaat aaattgtct gtgcttata atggtttca cccagtttt tgtacttaa attaaaaat aatctttta attttttgt actgttttt ggaagtata cttttgttt tctagtcat tcattgcgc atattttta tactgtgta gtacaatta ttttgtaca caataaatt tcttgatac atc
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