| General information | |||
| TC000314 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007417.3: 13136603..13139590 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs | TC000314 | ||
GO terms for Tribolium
-
{{ goTerm.term }} ({{ goTerm.evidence }}){{ goTerm.evidenceTooltip }}{{goTerm.showAll ? '[-]' : '[+]'}}Status: {{ goTerm.status }}
Evidence: {{ goTerm.evidence }} ({{ goTerm.evidenceTooltip }})
Reference: {{ goTerm.referenceText }}
Quotation: {{ goTerm.quotation }}
Submitter: {{ goTerm.name }}
There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
| Gene | {{ goGene }} |
|
|---|---|---|
| GO ID | * {{ blurIdResult }} |
|
| Evidence | {{ blurEvidenceResult }} |
|
| Reference | * {{ blurReferenceResult }} |
|
| Quotation | ||
| Name | ||
| Publish name | ||
| {{ unavailableText }} |
* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03226 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
||
Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC000314 RA
MGKSKGMTC AVKNCTSRY YGSNVSFFR FPLNDLDRL EKWKVACGR EELMLKSPT ELHTNFRVC GLHFDKKCI HNNRLISSS VPTLNLFQE ISVRLDHDY ILNEDQIFV GRYIEVGSP ASPTMGCEM STQTESEIQ KKHVGTQTE LFELSKWTM ETQTGKELS AATPRKRKL KAEIRSKGE EVKKLRRKL DIANKSLEE NEKNKKQEI SGEEFKQAC DRFLPPQFS KTAQVLSNL KNRKAQGRR FTGEFKQHA LGLYFASPK AYRIMVKKN LQLRLKSKN DNAKLCIIT MDEVSLKTH LFYDQKRDE IIGFHDIGK GKEFKPAQN ALVLMARGI EENWKQPLA FFFVNSTCN AQDLQSILH ECITRMLNI GFSVKAVVS DMGSNFIQL SRDLGITTK HTDFQVQGK TIRYFFDTP HLLKCMRNL LLQNTVHFD GKEASWKFV EQLFNLEKN KDLRMAPKL SEAHIHPTN FQKMKVKYA AQVLSSTVS SALSTYMAC GEISTLAMG TAEFIEKID HLFDILNSS TQASKNLYR RPYKGTPQQ DAFLHEMTQ FFKKIRVFS DTKTGKREI TNKIKSIKC FQVTIKSTM DLWEELKEE GCRNLKTRR INQDCLENF FGKIRQQGG NSVNPTPIQ FLRSFKKLC CVDLLQCAD TFNCAEDVD VLLMNNLPQ NIEISTEEQ ERTVPCQTG SLKITNTDF RGNDLPKKN VVHYISGYL VRKCLGKHT CPTCKEYAS AMDELDAST LFTHFKGHT EKYGGTGTT FGKLLMPAK PFVDFVETL EKALMKNIR WIACTANVQ DQLLQVLKE VQFSHPCSN FPVEYLLRL FGRMRCYYI LKYANRNFT DSSVKNRKL IILSHR
- >TC000314 RA
atgggtaag agcaaagga atgacatgt gcagtgaaa aattgcact tcacgttat tacggaagt aacgttagt ttttttaga ttcccattg aacgatttg gacaggttg gaaaaatgg aaagttgct tgtgggcgg gaggaactc atgttgaag agtcctacg gaacttcac accaatttc agggtctgt ggattgcat tttgacaaa aaatgcatc cacaataac aggttaata tctagctct gtgcctaca ttaaatttg tttcaggaa atttcagtg agactcgac cacgattac atcctaaat gaagaccag atctttgtt gggaggtac atcgaggta gggtctcca gccagtcca accatgggt tgtgagatg agcacccaa accgaatcg gagattcag aaaaagcat gttggaact cagactgaa ctgtttgag ctttccaaa tggacaatg gaaacccaa acaggtaag gaactttca gcggcaaca ccaaggaaa aggaagctg aaggcagaa atcagatcc aaaggagaa gaggtgaag aagttgagg aggaaactg gacatcgcg aacaagtcg ttggaagaa aacgaaaaa aacaagaag caggaaatc tcgggagaa gaattcaag caagcgtgc gacagattc ctgccacca caattcagc aaaacagcc caagtcctc agcaatttg aaaaacagg aaagcacaa ggaaggcga ttcacaggg gaattcaaa caacacgct ctgggattg tactttgca agccccaaa gcgtatcga atcatggtt aaaaagaac ttgcagctg agactgaaa tccaaaaat gataatgcc aaattgtgc attatcaca atggacgaa gtctcgctc aagacacac ttattctac gaccagaag cgagacgag atcatcgga ttccacgac attggaaaa ggcaaagaa ttcaaacca gcacaaaac gcgttggtt ttgatggca cgaggaatt gaagaaaat tggaagcag ccactcgca tttttcttc gtcaattcg acttgcaat gcccaggat ttgcagtcg atccttcac gagtgcatt acgagaatg ttgaatata ggtttcagt gttaaggcc gtcgtgtct gacatggga tccaatttt attcaactt tctcgtgat ttgggtatc acaactaaa catactgat tttcaggtg caaggaaaa accatccgc tacttcttc gacacccca caccttttg aagtgcatg aggaatctt ctcttgcag aacaccgtc cactttgat ggaaaagaa gcatcatgg aagttcgtc gagcagttg ttcaacctg gagaagaac aaggacctc aggatggca ccaaagttg agtgaagct cacattcac ccaacaaat ttccagaag atgaaggtg aagtacgcg gcacaagtt cttagttct acagtttcg tctgccctt tcaacgtac atggcctgc ggagagatc tccaccttg gctatggga acagcggag ttcattgaa aagatagat catcttttc gacattttg aactccagc acccaagct tcgaaaaat ctttaccga cggccatac aaggggacc ccacagcag gacgcattt cttcatgaa atgacccaa tttttcaaa aaaattcgt gtcttttca gacacgaaa acaggtaag agagaaatc acgaataaa ataaaaagc attaaatgt tttcaggtc acaatcaaa tcaacgatg gacctgtgg gaagaattg aaagaagag ggatgccga aatttgaag acaagaaga atcaaccag gactgtttg gaaaatttc ttcggaaaa attcgtcag caaggtggc aattcagta aatccaacg ccaattcag tttcttcgt tcattcaag aaactgtgt tgcgtggat ttattgcaa tgcgccgac acattcaac tgcgccgag gatgtggac gtcttgctg atgaacaac ctcccacag aacatcgaa atttcaaca gaagagcaa gaaagaaca gtgccatgt caaacagga tctctcaag atcacaaac acagatttt cgaggaaac gatcttcct aagaaaaac gtcgttcac tacatcagt gggtacctg gtcaggaag tgtctgggc aaacacaca tgcccaacc tgcaaagag tacgcctca gcgatggat gaacttgac gcttctaca cttttcacg cacttcaag ggacacaca gaaaaatac ggtggcacg ggtactact tttggaaaa cttttaatg cctgcaaaa ccttttgta gatttcgtc gagacattg gaaaaggcc ctgatgaaa aacattcgt tggattgcc tgcacagcg aatgtccaa gatcaactc cttcaggtt ctgaaagaa gttcagttt tcacacccg tgcagtaat tttcctgtt gaatatttg ttaagactt tttggcagg atgcgatgt tattacatc ctcaaatat gccaacagg aactttaca gactcgtcg gtgaaaaat cgaaaactg atcattctt tcacatcgt tga
- >TC000314 RA
agtaattta ttagtaatt gtatttgtt ttataataa ctaaaaatt ctgtatcgt ttccaaaaa aatgggtaa gagcaaagg aatgacatg tgcagtgaa aaattgcac ttcacgtta ttacggaag taacgttag tttttttag attcccatt gaacgattt ggacaggtt ggaaaaatg gaaagttgc ttgtgggcg ggaggaact catgttgaa gagtcctac ggaacttca caccaattt cagggtctg tggattgca ttttgacaa aaaatgcat ccacaataa caggttaat atctagctc tgtgcctac attaaattt gtttcagga aatttcagt gagactcga ccacgatta catcctaaa tgaagacca gatctttgt tgggaggta catcgaggt agggtctcc agccagtcc aaccatggg ttgtgagat gagcaccca aaccgaatc ggagattca gaaaaagca tgttggaac tcagactga actgtttga gctttccaa atggacaat ggaaaccca aacaggtaa ggaactttc agcggcaac accaaggaa aaggaagct gaaggcaga aatcagatc caaaggaga agaggtgaa gaagttgag gaggaaact ggacatcgc gaacaagtc gttggaaga aaacgaaaa aaacaagaa gcaggaaat ctcgggaga agaattcaa gcaagcgtg cgacagatt cctgccacc acaattcag caaaacagc ccaagtcct cagcaattt gaaaaacag gaaagcaca aggaaggcg attcacagg ggaattcaa acaacacgc tctgggatt gtactttgc aagccccaa agcgtatcg aatcatggt taaaaagaa cttgcagct gagactgaa atccaaaaa tgataatgc caaattgtg cattatcac aatggacga agtctcgct caagacaca cttattcta cgaccagaa gcgagacga gatcatcgg attccacga cattggaaa aggcaaaga attcaaacc agcacaaaa cgcgttggt tttgatggc acgaggaat tgaagaaaa ttggaagca gccactcgc atttttctt cgtcaattc gacttgcaa tgcccagga tttgcagtc gatccttca cgagtgcat tacgagaat gttgaatat aggtttcag tgttaaggc cgtcgtgtc tgacatggg atccaattt tattcaact ttctcgtga tttgggtat cacaactaa acatactga ttttcaggt gcaaggaaa aaccatccg ctacttctt cgacacccc acacctttt gaagtgcat gaggaatct tctcttgca gaacaccgt ccactttga tggaaaaga agcatcatg gaagttcgt cgagcagtt gttcaacct ggagaagaa caaggacct caggatggc accaaagtt gagtgaagc tcacattca cccaacaaa tttccagaa gatgaaggt gaagtacgc ggcacaagt tcttagttc tacagtttc gtctgccct ttcaacgta catggcctg cggagagat ctccacctt ggctatggg aacagcgga gttcattga aaagataga tcatctttt cgacatttt gaactccag cacccaagc ttcgaaaaa tctttaccg acggccata caaggggac cccacagca ggacgcatt tcttcatga aatgaccca atttttcaa aaaaattcg tgtcttttc agacacgaa aacaggtaa gagagaaat cacgaataa aataaaaag cattaaatg ttttcaggt cacaatcaa atcaacgat ggacctgtg ggaagaatt gaaagaaga gggatgccg aaatttgaa gacaagaag aatcaacca ggactgttt ggaaaattt cttcggaaa aattcgtca gcaaggtgg caattcagt aaatccaac gccaattca gtttcttcg ttcattcaa gaaactgtg ttgcgtgga tttattgca atgcgccga cacattcaa ctgcgccga ggatgtgga cgtcttgct gatgaacaa cctcccaca gaacatcga aatttcaac agaagagca agaaagaac agtgccatg tcaaacagg atctctcaa gatcacaaa cacagattt tcgaggaaa cgatcttcc taagaaaaa cgtcgttca ctacatcag tgggtacct ggtcaggaa gtgtctggg caaacacac atgcccaac ctgcaaaga gtacgcctc agcgatgga tgaacttga cgcttctac acttttcac gcacttcaa gggacacac agaaaaata cggtggcac gggtactac ttttggaaa acttttaat gcctgcaaa accttttgt agatttcgt cgagacatt ggaaaaggc cctgatgaa aaacattcg ttggattgc ctgcacagc gaatgtcca agatcaact ccttcaggt tctgaaaga agttcagtt ttcacaccc gtgcagtaa ttttcctgt tgaatattt gttaagact ttttggcag gatgcgatg ttattacat cctcaaata tgccaacag gaactttac agactcgtc ggtgaaaaa tcgaaaact gatcattct ttcacatcg ttgaacaca gagagaaga ccattttta cagttttta atgataagt attggtata atttgtgta cgtaat
