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Gene information
General information
TC000314 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13136603..13139590
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC000314
    iBeetle screen iB_03226

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000314 RA
      MGKSKGMTC AVKNCTSRY YGSNVSFFR FPLNDLDRL 
EKWKVACGR EELMLKSPT ELHTNFRVC GLHFDKKCI 
HNNRLISSS VPTLNLFQE ISVRLDHDY ILNEDQIFV 
GRYIEVGSP ASPTMGCEM STQTESEIQ KKHVGTQTE 
LFELSKWTM ETQTGKELS AATPRKRKL KAEIRSKGE 
EVKKLRRKL DIANKSLEE NEKNKKQEI SGEEFKQAC 
DRFLPPQFS KTAQVLSNL KNRKAQGRR FTGEFKQHA 
LGLYFASPK AYRIMVKKN LQLRLKSKN DNAKLCIIT 
MDEVSLKTH LFYDQKRDE IIGFHDIGK GKEFKPAQN 
ALVLMARGI EENWKQPLA FFFVNSTCN AQDLQSILH 
ECITRMLNI GFSVKAVVS DMGSNFIQL SRDLGITTK 
HTDFQVQGK TIRYFFDTP HLLKCMRNL LLQNTVHFD 
GKEASWKFV EQLFNLEKN KDLRMAPKL SEAHIHPTN 
FQKMKVKYA AQVLSSTVS SALSTYMAC GEISTLAMG 
TAEFIEKID HLFDILNSS TQASKNLYR RPYKGTPQQ 
DAFLHEMTQ FFKKIRVFS DTKTGKREI TNKIKSIKC 
FQVTIKSTM DLWEELKEE GCRNLKTRR INQDCLENF 
FGKIRQQGG NSVNPTPIQ FLRSFKKLC CVDLLQCAD 
TFNCAEDVD VLLMNNLPQ NIEISTEEQ ERTVPCQTG 
SLKITNTDF RGNDLPKKN VVHYISGYL VRKCLGKHT 
CPTCKEYAS AMDELDAST LFTHFKGHT EKYGGTGTT 
FGKLLMPAK PFVDFVETL EKALMKNIR WIACTANVQ 
DQLLQVLKE VQFSHPCSN FPVEYLLRL FGRMRCYYI 
LKYANRNFT DSSVKNRKL IILSHR
    CDS sequence
    • >TC000314 RA
      atgggtaag agcaaagga atgacatgt gcagtgaaa 
aattgcact tcacgttat tacggaagt aacgttagt 
ttttttaga ttcccattg aacgatttg gacaggttg 
gaaaaatgg aaagttgct tgtgggcgg gaggaactc 
atgttgaag agtcctacg gaacttcac accaatttc 
agggtctgt ggattgcat tttgacaaa aaatgcatc 
cacaataac aggttaata tctagctct gtgcctaca 
ttaaatttg tttcaggaa atttcagtg agactcgac 
cacgattac atcctaaat gaagaccag atctttgtt 
gggaggtac atcgaggta gggtctcca gccagtcca 
accatgggt tgtgagatg agcacccaa accgaatcg 
gagattcag aaaaagcat gttggaact cagactgaa 
ctgtttgag ctttccaaa tggacaatg gaaacccaa 
acaggtaag gaactttca gcggcaaca ccaaggaaa 
aggaagctg aaggcagaa atcagatcc aaaggagaa 
gaggtgaag aagttgagg aggaaactg gacatcgcg 
aacaagtcg ttggaagaa aacgaaaaa aacaagaag 
caggaaatc tcgggagaa gaattcaag caagcgtgc 
gacagattc ctgccacca caattcagc aaaacagcc 
caagtcctc agcaatttg aaaaacagg aaagcacaa 
ggaaggcga ttcacaggg gaattcaaa caacacgct 
ctgggattg tactttgca agccccaaa gcgtatcga 
atcatggtt aaaaagaac ttgcagctg agactgaaa 
tccaaaaat gataatgcc aaattgtgc attatcaca 
atggacgaa gtctcgctc aagacacac ttattctac 
gaccagaag cgagacgag atcatcgga ttccacgac 
attggaaaa ggcaaagaa ttcaaacca gcacaaaac 
gcgttggtt ttgatggca cgaggaatt gaagaaaat 
tggaagcag ccactcgca tttttcttc gtcaattcg 
acttgcaat gcccaggat ttgcagtcg atccttcac 
gagtgcatt acgagaatg ttgaatata ggtttcagt 
gttaaggcc gtcgtgtct gacatggga tccaatttt 
attcaactt tctcgtgat ttgggtatc acaactaaa 
catactgat tttcaggtg caaggaaaa accatccgc 
tacttcttc gacacccca caccttttg aagtgcatg 
aggaatctt ctcttgcag aacaccgtc cactttgat 
ggaaaagaa gcatcatgg aagttcgtc gagcagttg 
ttcaacctg gagaagaac aaggacctc aggatggca 
ccaaagttg agtgaagct cacattcac ccaacaaat 
ttccagaag atgaaggtg aagtacgcg gcacaagtt 
cttagttct acagtttcg tctgccctt tcaacgtac 
atggcctgc ggagagatc tccaccttg gctatggga 
acagcggag ttcattgaa aagatagat catcttttc 
gacattttg aactccagc acccaagct tcgaaaaat 
ctttaccga cggccatac aaggggacc ccacagcag 
gacgcattt cttcatgaa atgacccaa tttttcaaa 
aaaattcgt gtcttttca gacacgaaa acaggtaag 
agagaaatc acgaataaa ataaaaagc attaaatgt 
tttcaggtc acaatcaaa tcaacgatg gacctgtgg 
gaagaattg aaagaagag ggatgccga aatttgaag 
acaagaaga atcaaccag gactgtttg gaaaatttc 
ttcggaaaa attcgtcag caaggtggc aattcagta 
aatccaacg ccaattcag tttcttcgt tcattcaag 
aaactgtgt tgcgtggat ttattgcaa tgcgccgac 
acattcaac tgcgccgag gatgtggac gtcttgctg 
atgaacaac ctcccacag aacatcgaa atttcaaca 
gaagagcaa gaaagaaca gtgccatgt caaacagga 
tctctcaag atcacaaac acagatttt cgaggaaac 
gatcttcct aagaaaaac gtcgttcac tacatcagt 
gggtacctg gtcaggaag tgtctgggc aaacacaca 
tgcccaacc tgcaaagag tacgcctca gcgatggat 
gaacttgac gcttctaca cttttcacg cacttcaag 
ggacacaca gaaaaatac ggtggcacg ggtactact 
tttggaaaa cttttaatg cctgcaaaa ccttttgta 
gatttcgtc gagacattg gaaaaggcc ctgatgaaa 
aacattcgt tggattgcc tgcacagcg aatgtccaa 
gatcaactc cttcaggtt ctgaaagaa gttcagttt 
tcacacccg tgcagtaat tttcctgtt gaatatttg 
ttaagactt tttggcagg atgcgatgt tattacatc 
ctcaaatat gccaacagg aactttaca gactcgtcg 
gtgaaaaat cgaaaactg atcattctt tcacatcgt 
tga
    mRNA sequence
    • >TC000314 RA
      agtaattta ttagtaatt gtatttgtt ttataataa 
ctaaaaatt ctgtatcgt ttccaaaaa aatgggtaa 
gagcaaagg aatgacatg tgcagtgaa aaattgcac 
ttcacgtta ttacggaag taacgttag tttttttag 
attcccatt gaacgattt ggacaggtt ggaaaaatg 
gaaagttgc ttgtgggcg ggaggaact catgttgaa 
gagtcctac ggaacttca caccaattt cagggtctg 
tggattgca ttttgacaa aaaatgcat ccacaataa 
caggttaat atctagctc tgtgcctac attaaattt 
gtttcagga aatttcagt gagactcga ccacgatta 
catcctaaa tgaagacca gatctttgt tgggaggta 
catcgaggt agggtctcc agccagtcc aaccatggg 
ttgtgagat gagcaccca aaccgaatc ggagattca 
gaaaaagca tgttggaac tcagactga actgtttga 
gctttccaa atggacaat ggaaaccca aacaggtaa 
ggaactttc agcggcaac accaaggaa aaggaagct 
gaaggcaga aatcagatc caaaggaga agaggtgaa 
gaagttgag gaggaaact ggacatcgc gaacaagtc 
gttggaaga aaacgaaaa aaacaagaa gcaggaaat 
ctcgggaga agaattcaa gcaagcgtg cgacagatt 
cctgccacc acaattcag caaaacagc ccaagtcct 
cagcaattt gaaaaacag gaaagcaca aggaaggcg 
attcacagg ggaattcaa acaacacgc tctgggatt 
gtactttgc aagccccaa agcgtatcg aatcatggt 
taaaaagaa cttgcagct gagactgaa atccaaaaa 
tgataatgc caaattgtg cattatcac aatggacga 
agtctcgct caagacaca cttattcta cgaccagaa 
gcgagacga gatcatcgg attccacga cattggaaa 
aggcaaaga attcaaacc agcacaaaa cgcgttggt 
tttgatggc acgaggaat tgaagaaaa ttggaagca 
gccactcgc atttttctt cgtcaattc gacttgcaa 
tgcccagga tttgcagtc gatccttca cgagtgcat 
tacgagaat gttgaatat aggtttcag tgttaaggc 
cgtcgtgtc tgacatggg atccaattt tattcaact 
ttctcgtga tttgggtat cacaactaa acatactga 
ttttcaggt gcaaggaaa aaccatccg ctacttctt 
cgacacccc acacctttt gaagtgcat gaggaatct 
tctcttgca gaacaccgt ccactttga tggaaaaga 
agcatcatg gaagttcgt cgagcagtt gttcaacct 
ggagaagaa caaggacct caggatggc accaaagtt 
gagtgaagc tcacattca cccaacaaa tttccagaa 
gatgaaggt gaagtacgc ggcacaagt tcttagttc 
tacagtttc gtctgccct ttcaacgta catggcctg 
cggagagat ctccacctt ggctatggg aacagcgga 
gttcattga aaagataga tcatctttt cgacatttt 
gaactccag cacccaagc ttcgaaaaa tctttaccg 
acggccata caaggggac cccacagca ggacgcatt 
tcttcatga aatgaccca atttttcaa aaaaattcg 
tgtcttttc agacacgaa aacaggtaa gagagaaat 
cacgaataa aataaaaag cattaaatg ttttcaggt 
cacaatcaa atcaacgat ggacctgtg ggaagaatt 
gaaagaaga gggatgccg aaatttgaa gacaagaag 
aatcaacca ggactgttt ggaaaattt cttcggaaa 
aattcgtca gcaaggtgg caattcagt aaatccaac 
gccaattca gtttcttcg ttcattcaa gaaactgtg 
ttgcgtgga tttattgca atgcgccga cacattcaa 
ctgcgccga ggatgtgga cgtcttgct gatgaacaa 
cctcccaca gaacatcga aatttcaac agaagagca 
agaaagaac agtgccatg tcaaacagg atctctcaa 
gatcacaaa cacagattt tcgaggaaa cgatcttcc 
taagaaaaa cgtcgttca ctacatcag tgggtacct 
ggtcaggaa gtgtctggg caaacacac atgcccaac 
ctgcaaaga gtacgcctc agcgatgga tgaacttga 
cgcttctac acttttcac gcacttcaa gggacacac 
agaaaaata cggtggcac gggtactac ttttggaaa 
acttttaat gcctgcaaa accttttgt agatttcgt 
cgagacatt ggaaaaggc cctgatgaa aaacattcg 
ttggattgc ctgcacagc gaatgtcca agatcaact 
ccttcaggt tctgaaaga agttcagtt ttcacaccc 
gtgcagtaa ttttcctgt tgaatattt gttaagact 
ttttggcag gatgcgatg ttattacat cctcaaata 
tgccaacag gaactttac agactcgtc ggtgaaaaa 
tcgaaaact gatcattct ttcacatcg ttgaacaca 
gagagaaga ccattttta cagttttta atgataagt 
attggtata atttgtgta cgtaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY