Status
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Gene information
General information
TC000314 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007417.3: 13136603..13139590
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC000314
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03226

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC000314 RA
      MGKSKGMTC AVKNCTSRY YGSNVSFFR FPLNDLDRL 
      EKWKVACGR EELMLKSPT ELHTNFRVC GLHFDKKCI 
      HNNRLISSS VPTLNLFQE ISVRLDHDY ILNEDQIFV 
      GRYIEVGSP ASPTMGCEM STQTESEIQ KKHVGTQTE 
      LFELSKWTM ETQTGKELS AATPRKRKL KAEIRSKGE 
      EVKKLRRKL DIANKSLEE NEKNKKQEI SGEEFKQAC 
      DRFLPPQFS KTAQVLSNL KNRKAQGRR FTGEFKQHA 
      LGLYFASPK AYRIMVKKN LQLRLKSKN DNAKLCIIT 
      MDEVSLKTH LFYDQKRDE IIGFHDIGK GKEFKPAQN 
      ALVLMARGI EENWKQPLA FFFVNSTCN AQDLQSILH 
      ECITRMLNI GFSVKAVVS DMGSNFIQL SRDLGITTK 
      HTDFQVQGK TIRYFFDTP HLLKCMRNL LLQNTVHFD 
      GKEASWKFV EQLFNLEKN KDLRMAPKL SEAHIHPTN 
      FQKMKVKYA AQVLSSTVS SALSTYMAC GEISTLAMG 
      TAEFIEKID HLFDILNSS TQASKNLYR RPYKGTPQQ 
      DAFLHEMTQ FFKKIRVFS DTKTGKREI TNKIKSIKC 
      FQVTIKSTM DLWEELKEE GCRNLKTRR INQDCLENF 
      FGKIRQQGG NSVNPTPIQ FLRSFKKLC CVDLLQCAD 
      TFNCAEDVD VLLMNNLPQ NIEISTEEQ ERTVPCQTG 
      SLKITNTDF RGNDLPKKN VVHYISGYL VRKCLGKHT 
      CPTCKEYAS AMDELDAST LFTHFKGHT EKYGGTGTT 
      FGKLLMPAK PFVDFVETL EKALMKNIR WIACTANVQ 
      DQLLQVLKE VQFSHPCSN FPVEYLLRL FGRMRCYYI 
      LKYANRNFT DSSVKNRKL IILSHR
    CDS sequence
    • >TC000314 RA
      atgggtaag agcaaagga atgacatgt gcagtgaaa 
      aattgcact tcacgttat tacggaagt aacgttagt 
      ttttttaga ttcccattg aacgatttg gacaggttg 
      gaaaaatgg aaagttgct tgtgggcgg gaggaactc 
      atgttgaag agtcctacg gaacttcac accaatttc 
      agggtctgt ggattgcat tttgacaaa aaatgcatc 
      cacaataac aggttaata tctagctct gtgcctaca 
      ttaaatttg tttcaggaa atttcagtg agactcgac 
      cacgattac atcctaaat gaagaccag atctttgtt 
      gggaggtac atcgaggta gggtctcca gccagtcca 
      accatgggt tgtgagatg agcacccaa accgaatcg 
      gagattcag aaaaagcat gttggaact cagactgaa 
      ctgtttgag ctttccaaa tggacaatg gaaacccaa 
      acaggtaag gaactttca gcggcaaca ccaaggaaa 
      aggaagctg aaggcagaa atcagatcc aaaggagaa 
      gaggtgaag aagttgagg aggaaactg gacatcgcg 
      aacaagtcg ttggaagaa aacgaaaaa aacaagaag 
      caggaaatc tcgggagaa gaattcaag caagcgtgc 
      gacagattc ctgccacca caattcagc aaaacagcc 
      caagtcctc agcaatttg aaaaacagg aaagcacaa 
      ggaaggcga ttcacaggg gaattcaaa caacacgct 
      ctgggattg tactttgca agccccaaa gcgtatcga 
      atcatggtt aaaaagaac ttgcagctg agactgaaa 
      tccaaaaat gataatgcc aaattgtgc attatcaca 
      atggacgaa gtctcgctc aagacacac ttattctac 
      gaccagaag cgagacgag atcatcgga ttccacgac 
      attggaaaa ggcaaagaa ttcaaacca gcacaaaac 
      gcgttggtt ttgatggca cgaggaatt gaagaaaat 
      tggaagcag ccactcgca tttttcttc gtcaattcg 
      acttgcaat gcccaggat ttgcagtcg atccttcac 
      gagtgcatt acgagaatg ttgaatata ggtttcagt 
      gttaaggcc gtcgtgtct gacatggga tccaatttt 
      attcaactt tctcgtgat ttgggtatc acaactaaa 
      catactgat tttcaggtg caaggaaaa accatccgc 
      tacttcttc gacacccca caccttttg aagtgcatg 
      aggaatctt ctcttgcag aacaccgtc cactttgat 
      ggaaaagaa gcatcatgg aagttcgtc gagcagttg 
      ttcaacctg gagaagaac aaggacctc aggatggca 
      ccaaagttg agtgaagct cacattcac ccaacaaat 
      ttccagaag atgaaggtg aagtacgcg gcacaagtt 
      cttagttct acagtttcg tctgccctt tcaacgtac 
      atggcctgc ggagagatc tccaccttg gctatggga 
      acagcggag ttcattgaa aagatagat catcttttc 
      gacattttg aactccagc acccaagct tcgaaaaat 
      ctttaccga cggccatac aaggggacc ccacagcag 
      gacgcattt cttcatgaa atgacccaa tttttcaaa 
      aaaattcgt gtcttttca gacacgaaa acaggtaag 
      agagaaatc acgaataaa ataaaaagc attaaatgt 
      tttcaggtc acaatcaaa tcaacgatg gacctgtgg 
      gaagaattg aaagaagag ggatgccga aatttgaag 
      acaagaaga atcaaccag gactgtttg gaaaatttc 
      ttcggaaaa attcgtcag caaggtggc aattcagta 
      aatccaacg ccaattcag tttcttcgt tcattcaag 
      aaactgtgt tgcgtggat ttattgcaa tgcgccgac 
      acattcaac tgcgccgag gatgtggac gtcttgctg 
      atgaacaac ctcccacag aacatcgaa atttcaaca 
      gaagagcaa gaaagaaca gtgccatgt caaacagga 
      tctctcaag atcacaaac acagatttt cgaggaaac 
      gatcttcct aagaaaaac gtcgttcac tacatcagt 
      gggtacctg gtcaggaag tgtctgggc aaacacaca 
      tgcccaacc tgcaaagag tacgcctca gcgatggat 
      gaacttgac gcttctaca cttttcacg cacttcaag 
      ggacacaca gaaaaatac ggtggcacg ggtactact 
      tttggaaaa cttttaatg cctgcaaaa ccttttgta 
      gatttcgtc gagacattg gaaaaggcc ctgatgaaa 
      aacattcgt tggattgcc tgcacagcg aatgtccaa 
      gatcaactc cttcaggtt ctgaaagaa gttcagttt 
      tcacacccg tgcagtaat tttcctgtt gaatatttg 
      ttaagactt tttggcagg atgcgatgt tattacatc 
      ctcaaatat gccaacagg aactttaca gactcgtcg 
      gtgaaaaat cgaaaactg atcattctt tcacatcgt 
      tga
    mRNA sequence
    • >TC000314 RA
      agtaattta ttagtaatt gtatttgtt ttataataa 
      ctaaaaatt ctgtatcgt ttccaaaaa aatgggtaa 
      gagcaaagg aatgacatg tgcagtgaa aaattgcac 
      ttcacgtta ttacggaag taacgttag tttttttag 
      attcccatt gaacgattt ggacaggtt ggaaaaatg 
      gaaagttgc ttgtgggcg ggaggaact catgttgaa 
      gagtcctac ggaacttca caccaattt cagggtctg 
      tggattgca ttttgacaa aaaatgcat ccacaataa 
      caggttaat atctagctc tgtgcctac attaaattt 
      gtttcagga aatttcagt gagactcga ccacgatta 
      catcctaaa tgaagacca gatctttgt tgggaggta 
      catcgaggt agggtctcc agccagtcc aaccatggg 
      ttgtgagat gagcaccca aaccgaatc ggagattca 
      gaaaaagca tgttggaac tcagactga actgtttga 
      gctttccaa atggacaat ggaaaccca aacaggtaa 
      ggaactttc agcggcaac accaaggaa aaggaagct 
      gaaggcaga aatcagatc caaaggaga agaggtgaa 
      gaagttgag gaggaaact ggacatcgc gaacaagtc 
      gttggaaga aaacgaaaa aaacaagaa gcaggaaat 
      ctcgggaga agaattcaa gcaagcgtg cgacagatt 
      cctgccacc acaattcag caaaacagc ccaagtcct 
      cagcaattt gaaaaacag gaaagcaca aggaaggcg 
      attcacagg ggaattcaa acaacacgc tctgggatt 
      gtactttgc aagccccaa agcgtatcg aatcatggt 
      taaaaagaa cttgcagct gagactgaa atccaaaaa 
      tgataatgc caaattgtg cattatcac aatggacga 
      agtctcgct caagacaca cttattcta cgaccagaa 
      gcgagacga gatcatcgg attccacga cattggaaa 
      aggcaaaga attcaaacc agcacaaaa cgcgttggt 
      tttgatggc acgaggaat tgaagaaaa ttggaagca 
      gccactcgc atttttctt cgtcaattc gacttgcaa 
      tgcccagga tttgcagtc gatccttca cgagtgcat 
      tacgagaat gttgaatat aggtttcag tgttaaggc 
      cgtcgtgtc tgacatggg atccaattt tattcaact 
      ttctcgtga tttgggtat cacaactaa acatactga 
      ttttcaggt gcaaggaaa aaccatccg ctacttctt 
      cgacacccc acacctttt gaagtgcat gaggaatct 
      tctcttgca gaacaccgt ccactttga tggaaaaga 
      agcatcatg gaagttcgt cgagcagtt gttcaacct 
      ggagaagaa caaggacct caggatggc accaaagtt 
      gagtgaagc tcacattca cccaacaaa tttccagaa 
      gatgaaggt gaagtacgc ggcacaagt tcttagttc 
      tacagtttc gtctgccct ttcaacgta catggcctg 
      cggagagat ctccacctt ggctatggg aacagcgga 
      gttcattga aaagataga tcatctttt cgacatttt 
      gaactccag cacccaagc ttcgaaaaa tctttaccg 
      acggccata caaggggac cccacagca ggacgcatt 
      tcttcatga aatgaccca atttttcaa aaaaattcg 
      tgtcttttc agacacgaa aacaggtaa gagagaaat 
      cacgaataa aataaaaag cattaaatg ttttcaggt 
      cacaatcaa atcaacgat ggacctgtg ggaagaatt 
      gaaagaaga gggatgccg aaatttgaa gacaagaag 
      aatcaacca ggactgttt ggaaaattt cttcggaaa 
      aattcgtca gcaaggtgg caattcagt aaatccaac 
      gccaattca gtttcttcg ttcattcaa gaaactgtg 
      ttgcgtgga tttattgca atgcgccga cacattcaa 
      ctgcgccga ggatgtgga cgtcttgct gatgaacaa 
      cctcccaca gaacatcga aatttcaac agaagagca 
      agaaagaac agtgccatg tcaaacagg atctctcaa 
      gatcacaaa cacagattt tcgaggaaa cgatcttcc 
      taagaaaaa cgtcgttca ctacatcag tgggtacct 
      ggtcaggaa gtgtctggg caaacacac atgcccaac 
      ctgcaaaga gtacgcctc agcgatgga tgaacttga 
      cgcttctac acttttcac gcacttcaa gggacacac 
      agaaaaata cggtggcac gggtactac ttttggaaa 
      acttttaat gcctgcaaa accttttgt agatttcgt 
      cgagacatt ggaaaaggc cctgatgaa aaacattcg 
      ttggattgc ctgcacagc gaatgtcca agatcaact 
      ccttcaggt tctgaaaga agttcagtt ttcacaccc 
      gtgcagtaa ttttcctgt tgaatattt gttaagact 
      ttttggcag gatgcgatg ttattacat cctcaaata 
      tgccaacag gaactttac agactcgtc ggtgaaaaa 
      tcgaaaact gatcattct ttcacatcg ttgaacaca 
      gagagaaga ccattttta cagttttta atgataagt 
      attggtata atttgtgta cgtaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY