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Gene information
General information
TC001631 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1257639..1261257
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.

    GO terms for the homologous Drosophila genes

    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.

    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
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    iBeetle screen iB_03224

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 1 (3 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Left: Right:
    Protein sequence
    • >TC001631 RA
    CDS sequence
    • >TC001631 RA
      atgagtttc ttcctttgt tataaatgt agcactaac 
      caggaaaag atagtagag ttagactat tctcgttgt 
      gggatttcc gaagtgcct gtcgatgtc tacaacaac 
      tccccgact ctagaagtg ctgcatctt gaggggaat 
      aaattgaag gatctttcg cctcagctg ttccaatgc 
      atagatctg aggtatcta aacgtcagc gacaacgaa 
      atcaggtcc attccgccg ctcatttcg aagctgaac 
      agcctgcaa gtcctaatt ttcagtaaa aatgcgctg 
      gttttggag ggagtttcg ccgaacatt gacaaactg 
      aacaagctg acgatactc gacttgagc atgaatgac 
      ttggggaag gtgcccgaa gccataatg agcctgatt 
      aatttgcaa cagctgtgc ttgaacgat acaggaatt 
      gattatgtg cctgccaat attggacgt ctttccaac 
      cttagaatc ctagagtta agggataat agcttgcga 
      gaattgcct aagtctatc aggagacta accaattta 
      caaagactt gacgttagc gacaataac ttgtcccaa 
      ttgactgaa gtttgtgaa tctcacggt aacttgact 
      gagttgtgg atcaacggt aacaatatc acgaaactg 
      tcaccgtca atcacgcat ttgaaaaaa atgaacgac 
      ttcgatgct tcctacaat aatctacaa accatacca 
      aaagaaata ggtcaatgg actaaaata acgaactta 
      attttaagc tttaatcaa atctctgtg ttgccaaaa 
      gctatagga aatttacgc aatctacaa gtattaaaa 
      ctcgaatcg aataattta gaggaactg ccaaacacg 
      attagcaaa ctcacaaat ctggaagaa ctcaacttg 
      cagaataac ttcatcatt aaacttccg tctggaatc 
      gggcatttg cgcaaattg gcaactctc atactttcg 
      gataacaag ctggaacag ttaccacca gaaatcgga 
      agttgctgc tccctcacg attttaaac gtccacaac 
      aactactta caccgccta ccggacgaa gtgggtcat 
      ttacaaaag ctcacaact ttgggcctc atcggcaac 
      aaattggaa tatttaccg ataacagta tcaaaacta 
      acgaatttg aaagcactt tggctgaca cccaaccag 
      acgcagcct ttgatccat ttacaaaac gagcaactc 
      cccgacggc caagtcgtc ctgacctca gtcctgttc 
      ccgcaagtg cccctcccg gaggccccg cccccggtg 
      cacctccct atcagcacc cagagctac cacatcacc 
      ttcaacatg gacaacatc cccaccgag gcctcccta 
      agccgcacc cccacgccc caccacaag gaactgcgc 
      cgtttgcgc aacatcctg cgcgggggc gaggccccc 
      gaaagccag cccccgaac atctcggag atcaaggag 
      gccaaagtg acgcacatt tcgggcgat ttgtccgtg 
      tcccagtcc agtttattg gacaaagag ccggagaat 
      agcgaaacc gcggtggtt tataaggcg gcgaaggag 
      gaggagctt ccgaagcaa ccgccgccg tatcacatt 
      gcagccgcg tattcgaag aacgcccac ttgttcgcg 
      cagatttgc ccgcccagc ccgcacgac caacccaag 
      cagttcagc caacaacag ccggcgaat atccaccat 
      ccgacgccc aagttggcc aaaaaccgg gagcaaatg 
      caggagttt tacgacgag ggtaagaaa ccgaagtgg 
      ccttttgga gcgcaccag atttgcaag gtgattgag 
      gtggaactg gtggggtat cgcgttttg ggcgatttt 
      cacgttgtt gcgaacgct gatggtgtt tatgtcgat 
      gaggtccat ccggccggc gttgcatat cagaagttg 
      tttccaggg gacaaaata ctcagtttc gatgacgtt 
      gatttcacc gtcatggat tcgcaaagt gcttacgaa 
      tatgcttac caaaaaatc gggacggtt aaggttgta 
      accgtctcg cgcaaacaa gtgtcttga
    mRNA sequence
    • >TC001631 RA
      gcctggtcc aaatgatca gaaatgttt tatttttaa 
      aaatgtaac ctcataaat tcggaaaga tgaattgga 
      gatgaaaag tatgaccag tgtggctat aaaatgagt 
      ttcttcctt tgttataaa tgtagcact aaccaggaa 
      aagatagta gagttagac tattctcgt tgtgggatt 
      tccgaagtg cctgtcgat gtctacaac aactccccg 
      actctagaa gtgctgcat cttgagggg aataaattg 
      aaggatctt tcgcctcag ctgttccaa tgcatagat 
      ctgaggtat ctaaacgtc agcgacaac gaaatcagg 
      tccattccg ccgctcatt tcgaagctg aacagcctg 
      caagtccta attttcagt aaaaatgcg ctggttttg 
      gagggagtt tcgccgaac attgacaaa ctgaacaag 
      ctgacgata ctcgacttg agcatgaat gacttgggg 
      aaggtgccc gaagccata atgagcctg attaatttg 
      caacagctg tgcttgaac gatacagga attgattat 
      gtgcctgcc aatattgga cgtctttcc aaccttaga 
      atcctagag ttaagggat aatagcttg cgagaattg 
      cctaagtct atcaggaga ctaaccaat ttacaaaga 
      cttgacgtt agcgacaat aacttgtcc caattgact 
      gaagtttgt gaatctcac ggtaacttg actgagttg 
      tggatcaac ggtaacaat atcacgaaa ctgtcaccg 
      tcaatcacg catttgaaa aaaatgaac gacttcgat 
      gcttcctac aataatcta caaaccata ccaaaagaa 
      ataggtcaa tggactaaa ataacgaac ttaatttta 
      agctttaat caaatctct gtgttgcca aaagctata 
      ggaaattta cgcaatcta caagtatta aaactcgaa 
      tcgaataat ttagaggaa ctgccaaac acgattagc 
      aaactcaca aatctggaa gaactcaac ttgcagaat 
      aacttcatc attaaactt ccgtctgga atcgggcat 
      ttgcgcaaa ttggcaact ctcatactt tcggataac 
      aagctggaa cagttacca ccagaaatc ggaagttgc 
      tgctccctc acgatttta aacgtccac aacaactac 
      ttacaccgc ctaccggac gaagtgggt catttacaa 
      aagctcaca actttgggc ctcatcggc aacaaattg 
      gaatattta ccgataaca gtatcaaaa ctaacgaat 
      ttgaaagca ctttggctg acacccaac cagacgcag 
      cctttgatc catttacaa aacgagcaa ctccccgac 
      ggccaagtc gtcctgacc tcagtcctg ttcccgcaa 
      gtgcccctc ccggaggcc ccgcccccg gtgcacctc 
      cctatcagc acccagagc taccacatc accttcaac 
      atggacaac atccccacc gaggcctcc ctaagccgc 
      acccccacg ccccaccac aaggaactg cgccgtttg 
      cgcaacatc ctgcgcggg ggcgaggcc cccgaaagc 
      cagcccccg aacatctcg gagatcaag gaggccaaa 
      gtgacgcac atttcgggc gatttgtcc gtgtcccag 
      tccagttta ttggacaaa gagccggag aatagcgaa 
      accgcggtg gtttataag gcggcgaag gaggaggag 
      cttccgaag caaccgccg ccgtatcac attgcagcc 
      gcgtattcg aagaacgcc cacttgttc gcgcagatt 
      tgcccgccc agcccgcac gaccaaccc aagcagttc 
      agccaacaa cagccggcg aatatccac catccgacg 
      cccaagttg gccaaaaac cgggagcaa atgcaggag 
      ttttacgac gagggtaag aaaccgaag tggcctttt 
      ggagcgcac cagatttgc aaggtgatt gaggtggaa 
      ctggtgggg tatcgcgtt ttgggcgat tttcacgtt 
      gttgcgaac gctgatggt gtttatgtc gatgaggtc 
      catccggcc ggcgttgca tatcagaag ttgtttcca 
      ggggacaaa atactcagt ttcgatgac gttgatttc 
      accgtcatg gattcgcaa agtgcttac gaatatgct 
      taccaaaaa atcgggacg gttaaggtt gtaaccgtc 
      tcgcgcaaa caagtgtct tgacaaaga gtatttttt 
      tagattagt tgcatgcct aatactcct agcgaggag 
      agtgtttca cttgcattt aacattgtt tatgttacc 
      tcatagttg cgtttttga tcatttaac tagaggctg 
      tgatttagt ttaagttac attaactca agagtattt 
      gttatttat ttatgtaca agcgtatga taattagct 
      ctctaattt tctatgact aaattgtct ttttatatt 
      tctaaaata aattatacg attgaat
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY