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Gene information
General information
TC001624 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

scaffold_seqid2: 1083254..1086633
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001624
    iBeetle screen iB_03222
    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 10.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001624 RA
      MSGAVYGGD EVGALVFDI GHNSTRAGF GGEDSPKLD 
      IPSTVGVWL DSHDEISQK RYNIGITSI NVRRSEMEL 
      TTFLKDGMV ENWEIFENF LDYIYSNAL RAIPNDHPL 
      LITEPPWVT APKREQLTE LMFEKYNVP ALYLAKNAS 
      LAAFANGRP TCLVVDSGA THTSAVPVH DGFVLTQAV 
      VKSPAGGDY LSTQCRNYL TEKGIEIVP PCMVASKEV 
      TKAEEAPNF KRRLFHAKL TDSWHNYMI KQVMQDFQA 
      SVLQVSDNL YDEEVVKTM PAIHYEFPN GFHKDFGVE 
      RFKIPEPLF NPIAGIKSG IQPTLGVGP LVTTSVGMC 
      DIDIRPAMY GSVVVTGGN SNLQGFTER LNRDLAAKT 
      PPSMRLKII SAPGATERK FGAWSGGSI LTSLGSFQQ 
      LWVSKQEYD DGGKAIVQS KCL
    CDS sequence
    • >TC001624 RA
      atgagtggg gctgtttac gggggcgac gaagttggg 
      gcccttgtc ttcgacata ggccacaac tcgaccagg 
      gcgggattc gggggtgag gatagcccc aagctggac 
      ataccctcg actgtgggg gtctggctg gactctcat 
      gacgagatc agccagaag cgctacaat atcggcatt 
      acttcgatc aacgttagg agatccgag atggagctg 
      acgacgttt ttgaaagac ggtatggtt gagaactgg 
      gagatattt gagaatttt ttggattat atatacagt 
      aacgctttg cgggcgatc ccgaacgat cacccgttg 
      cttatcact gagccgcca tgggtaacc gcccctaag 
      agggagcaa ttaactgag ttaatgttt gagaagtac 
      aatgttcct gctctttat ttagctaag aacgcatcc 
      ctcgctgcc ttcgccaat gggcgtccg acttgcctt 
      gttgttgat agcggagct acgcacaca tccgctgtt 
      cctgtacat gacggtttt gtgctgaca caagctgtg 
      gttaagtct ccagctggt ggagactat ttgagcaca 
      caatgtcga aattattta actgagaaa ggaattgaa 
      attgtccct ccttgcatg gtagcctcg aaagaagtt 
      accaaagcc gaagaagcg cccaatttc aagcgacgg 
      ctcttccat gccaagctc accgactcc tggcacaac 
      tacatgatc aaacaagtt atgcaagac ttccaagct 
      tcagtatta caagtctcc gacaatttg tacgacgaa 
      gaagttgtt aaaacaatg cccgcgatt cattacgaa 
      ttcccgaac ggttttcat aaggatttc ggtgttgaa 
      cgctttaaa atccctgaa ccgcttttc aaccctatt 
      gcaggcatt aaatcaggc attcagccc actctaggg 
      gttggccct ttggtcaca accagtgtg ggtatgtgc 
      gacattgac atcagaccg gccatgtat ggaagtgtc 
      gtagtgact ggcggtaat tcaaatttg caaggattt 
      accgagcgg ttaaaccgc gatttagct gctaaaaca 
      cctccgagt atgcgcttg aaaataatt agtgctcct 
      ggagccacc gaacgcaaa ttcggggct tggtcgggc 
      ggctcgatt ttgacatct ttgggttca ttccagcaa 
      ctttgggtg tcaaagcaa gagtatgac gacgggggc 
      aaggccata gtgcaatca aagtgtttg taa
    mRNA sequence
    • >TC001624 RA
      atgagtggg gctgtttac gggggcgac gaagttggg 
      gcccttgtc ttcgacata ggccacaac tcgaccagg 
      gcgggattc gggggtgag gatagcccc aagctggac 
      ataccctcg actgtgggg gtctggctg gactctcat 
      gacgagatc agccagaag cgctacaat atcggcatt 
      acttcgatc aacgttagg agatccgag atggagctg 
      acgacgttt ttgaaagac ggtatggtt gagaactgg 
      gagatattt gagaatttt ttggattat atatacagt 
      aacgctttg cgggcgatc ccgaacgat cacccgttg 
      cttatcact gagccgcca tgggtaacc gcccctaag 
      agggagcaa ttaactgag ttaatgttt gagaagtac 
      aatgttcct gctctttat ttagctaag aacgcatcc 
      ctcgctgcc ttcgccaat gggcgtccg acttgcctt 
      gttgttgat agcggagct acgcacaca tccgctgtt 
      cctgtacat gacggtttt gtgctgaca caagctgtg 
      gttaagtct ccagctggt ggagactat ttgagcaca 
      caatgtcga aattattta actgagaaa ggaattgaa 
      attgtccct ccttgcatg gtagcctcg aaagaagtt 
      accaaagcc gaagaagcg cccaatttc aagcgacgg 
      ctcttccat gccaagctc accgactcc tggcacaac 
      tacatgatc aaacaagtt atgcaagac ttccaagct 
      tcagtatta caagtctcc gacaatttg tacgacgaa 
      gaagttgtt aaaacaatg cccgcgatt cattacgaa 
      ttcccgaac ggttttcat aaggatttc ggtgttgaa 
      cgctttaaa atccctgaa ccgcttttc aaccctatt 
      gcaggcatt aaatcaggc attcagccc actctaggg 
      gttggccct ttggtcaca accagtgtg ggtatgtgc 
      gacattgac atcagaccg gccatgtat ggaagtgtc 
      gtagtgact ggcggtaat tcaaatttg caaggattt 
      accgagcgg ttaaaccgc gatttagct gctaaaaca 
      cctccgagt atgcgcttg aaaataatt agtgctcct 
      ggagccacc gaacgcaaa ttcggggct tggtcgggc 
      ggctcgatt ttgacatct ttgggttca ttccagcaa 
      ctttgggtg tcaaagcaa gagtatgac gacgggggc 
      aaggccata gtgcaatca aagtgtttg taa