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Gene information
General information
TC001612 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1323561..1325761
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001612
    iBeetle screen iB_03219

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 2 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001612 RA
      MGCAVSTTG DKEATERSK KIDRDLRAD GERAASEVK 
      LLLLGAGES GKSTIVKQM KIIHETGYS KEECEQYRP 
      VVYSNTIQS LMAIIRAMG QLRIDFADP SRADIARQF 
      FTYASAAEE GELTPELVM LMKKLWQDP GVQLCFARS 
      REYQLNDSA AYYLNALDR ISQPNYIPS QQDVLRTRV 
      KTTGIVETH FSFKGLHFK MFDVGGQRS ERKKWIHCF 
      EGVTAIIFC VALSGYDLV LAEDEEMNR MVESMKLFD 
      SICNSKWFV STSIILFLN KKDLFEQKI TRSPLTICF 
      PEYTGPNTY EDASAYIRM KFENLNKRK DQKEIYTHF 
      TCATDTTNI QFVFDAVTD VIINNNLKD CGLLM
    CDS sequence
    • >TC001612 RA
      atgggttgt gcggtcagt acaacgggc gataaagaa 
      gctacagaa cggtcgaag aaaattgat agagacctt 
      cgagccgat ggcgaaagg gcagcaagc gaagtcaag 
      ctgttgctg cttggcgcc ggcgaatca ggcaagagc 
      acaatcgtc aaacaaatg aagatcata cacgagacg 
      ggctactcg aaggaggaa tgcgaacag tacaggccc 
      gttgtttac agtaacact attcagagc ctaatggcc 
      atcattcgg gccatgggt cagttgcga atcgatttc 
      gctgatcct agccgagct gacatcgcg cgccagttc 
      ttcacatac gctagtgca gccgaggag ggcgaactg 
      acccccgaa cttgtgatg ttgatgaag aagctgtgg 
      caggaccct ggagtgcag ttatgcttc gcccgctcc 
      cgggaatac caactgaac gactccgca gcttattat 
      ttaaacgct ttagaccga atttcccaa cccaattac 
      attccctcc cagcaggac gtcctgcga actagagtc 
      aaaacgacg ggaatcgtg gaaacgcac ttttcgttc 
      aaaggcctt cacttcaag atgttcgat gtcgggggc 
      caacgctcg gagcgcaag aaatggata cattgtttc 
      gagggcgtg actgcgatc attttttgc gtggccttg 
      tctgggtac gacctggtt ttggccgag gatgaggag 
      atgaacaga atggtggag tcgatgaaa ctgttcgac 
      tcgatttgc aacagcaag tggttcgtg tcgacttcg 
      atcatcctg tttctgaat aagaaggat ttgttcgag 
      cagaagata acgcgaagt ccgttgacg atatgcttt 
      cccgaatat accgggccg aatacttac gaagacgcg 
      tcggcttat atccggatg aagttcgag aacttgaac 
      aagcggaaa gatcagaag gagatttat actcatttc 
      acgtgtgcc acggacact acgaatatc cagtttgtg 
      ttcgacgcg gttactgat gttattatt aataataat 
      ttaaaagat tgcggcctt ttgatgtaa
    mRNA sequence
    • >TC001612 RA
      caagttttt ctccagcgg gtggagtcg tttgtgact 
      gttacagca acaagtgtc aaaataaaa gcgatttta 
      aatataata tgggttgtg cggtcagta caacgggcg 
      ataaagaag ctacagaac ggtcgaaga aaattgata 
      gagaccttc gagccgatg gcgaaaggg cagcaagcg 
      aagtcaagc tgttgctgc ttggcgccg gcgaatcag 
      gcaagagca caatcgtca aacaaatga agatcatac 
      acgagacgg gctactcga aggaggaat gcgaacagt 
      acaggcccg ttgtttaca gtaacacta ttcagagcc 
      taatggcca tcattcggg ccatgggtc agttgcgaa 
      tcgatttcg ctgatccta gccgagctg acatcgcgc 
      gccagttct tcacatacg ctagtgcag ccgaggagg 
      gcgaactga cccccgaac ttgtgatgt tgatgaaga 
      agctgtggc aggaccctg gagtgcagt tatgcttcg 
      cccgctccc gggaatacc aactgaacg actccgcag 
      cttattatt taaacgctt tagaccgaa tttcccaac 
      ccaattaca ttccctccc agcaggacg tcctgcgaa 
      ctagagtca aaacgacgg gaatcgtgg aaacgcact 
      tttcgttca aaggccttc acttcaaga tgttcgatg 
      tcgggggcc aacgctcgg agcgcaaga aatggatac 
      attgtttcg agggcgtga ctgcgatca ttttttgcg 
      tggccttgt ctgggtacg acctggttt tggccgagg 
      atgaggaga tgaacagaa tggtggagt cgatgaaac 
      tgttcgact cgatttgca acagcaagt ggttcgtgt 
      cgacttcga tcatcctgt ttctgaata agaaggatt 
      tgttcgagc agaagataa cgcgaagtc cgttgacga 
      tatgctttc ccgaatata ccgggccga atacttacg 
      aagacgcgt cggcttata tccggatga agttcgaga 
      acttgaaca agcggaaag atcagaagg agatttata 
      ctcatttca cgtgtgcca cggacacta cgaatatcc 
      agtttgtgt tcgacgcgg ttactgatg ttattatta 
      ataataatt taaaagatt gcggccttt tgatgtaac 
      ggcctattt gataggttt atgcgaggc atttcagtt 
      tatgcaatg tataaaacg gaagggaag ctgcttgaa 
      gcggcactg atctcgttt cgtctattc cctcctctt 
      tcagtgata aaattcatt cagtattgt gttttgttt 
      cacaagcag cttctcttc tcgcgttgt cttataaag 
      atatgtatt tatttttaa cgtaagtca ttacaagtt 
      atgcaatat tttaacaca catttacac acgacaagt 
      ggcgtaacg taaaactgc caaactgtt ctgttgttt 
      tatatcgac aaatttata agagtaaga gtttacatg 
      ttatatatc tgtataaat tgccaaaag atgctcaca 
      tgccatatg tatagtaag atattttta taacgatgt 
      tgaataacg tttcccgaa tattttttg acagttttt 
      ctacataca tctgcctta acagtgaca atttcaagt 
      aagaaaaca gtttttatt tcattgtgt agtagattg 
      gcatcaagt aggtttctc gtgtaatta tgtcattgg 
      ttagaactg tacttactg tatcaaaga cttattttt 
      tatactttt gtatgttca caaagtaac aaaaatagt 
      aattaattt ttggaaaat atttacgcg ttttggcgc 
      cttttcccc aaaatgcgt aaataatga gacgcacca 
      caggtcagg tcaaataaa cattgttta ttttgtacc 
      taatgtttt gtaaagtaa caaatatat gacgccatc 
      catg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY