| General information | |||
| TC001607 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007416.3: 1268719..1275997 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001607 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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| GO ID | * {{ blurIdResult }} |
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| Reference | * {{ blurReferenceResult }} |
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03218 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001607 RA
MVGVCYLAT IIIYIISSA SCTKSKDCP EICTCLGSY VDCSSKRLQ VIPSNLPKW TTHLDLRNN TIKKLNDVT WRNLTILTE LILNKNEIS VIPNDAFSN QKQLKILEL NRNQLKSIE ALTFKSLER LYVLKLKRN QITQLKDGA FYGLLSIDK LILDYNHIL VISKGWLYG LQSLKELSL NHNYINFVE PEAWEFCKK LALLDLSNN RLESVAANT FKHLNDLQK LVLSNNKIT FIEERAFSH LPNLKYLHL NNNKISWTI EDANGVFQG LGNLIKFYL ADNNIKSIS KNAFIGLKN VTYLNLNDN NITSIQMNA FSEVPLLSD LVINTTYLL CDCNIRWFY EWLDTKQFK IRAICAYPE WLRGQSLVE IPTSNFTCD ELPKPRLTE EPEPEIMAL KGENITLSC TAMSSSNSP MTFQWKKDN VDIINMNIN ENSSSDGKT TEAVSKLNI YRVQHSNAG KYQCVVSNI FGTTYSQKS SISVLIFPT FLKRPDNVT VKAGDVAKL VCAAHGEPQ PEIAWQKDG GNDFPAARE RRMHVLPAD DSFFISNVK PTDMGIYSC TAHNAAGTI VTNASLTVY EKPSFVKSM ENKEVTAGE LVVLQCMAS GTPKPTITW YKNGEPIRA TERHFFTAE EQLMIIVDT VQSDSGKYE CHLNNSLGE KTGYSLLRV KPGNFTTTD MMGIIIITV VCCAVATSI IWVVIIYQT RRRMPPPAA PPLEGPLEL DRGVQFTDN ASDRSSCRD SGTGDSARR SNDDLAHED YKLIINEEP QPGWNVMRI ASLTYIPAD PNSSHAPLL PGTPYVRST NHDRPETPD KGAPPAEQS ETTD
- >TC001607 RA
atggtgggt gtttgttat ttggctacg atcattatt tacatcatt agttcggca agttgtacg aaaagcaaa gattgtcca gaaatttgc acttgtttg ggttcttac gtcgactgt tcgtcgaaa agattgcaa gtaattcca tcgaatctt ccaaaatgg accactcac ctcgattta cgtaataac accattaaa aaacttaac gacgttact tggagaaac ctgactatc ttaactgaa ctcatattg aacaaaaac gaaatttct gttattccc aacgacgcg ttcagtaac cagaagcag ctaaaaatt cttgaactt aaccgaaat cagctgaag tccattgaa gctttaact ttcaagtca ttagaacgt ctatatgta ttaaaattg aaacgaaac caaataacg caactcaaa gatggggcc ttctacgga ttgttgtct attgacaaa ttgattttg gattacaat cacatatta gtcatttcg aaaggatgg ctttatggt ttacagtct ctaaaagaa ttgtctttg aaccacaat tacatcaat tttgtcgaa cctgaggct tgggagttt tgtaaaaaa ctggccctc ttagacttg tctaataac aggctagaa tcggtcgca gctaatacg ttcaaacat cttaacgat ctccaaaaa ctagtatta agtaacaat aagataaca tttattgaa gaaagagct tttagccac ttgccaaat ctgaaatac ttgcattta aataacaat aaaatctcg tggacgatt gaggatgcg aacggcgtg ttccaagga ctgggcaat ttgattaag ttttacctg gccgataat aacattaaa tcgataagt aaaaacgcc tttattgga ttgaagaac gtaacgtat ttaaatttg aacgacaat aacattacc tcaatccaa atgaacgcc ttctctgaa gtgcctttg ctttcggat ctcgttatt aacaccact tatttactt tgcgactgt aacattcgt tggttttac gaatggttg gacacaaaa cagttcaaa atccgtgcg atttgtgcc tatcccgaa tggttgcga ggccagtca ttggtcgaa ataccaaca tcgaatttt acatgtgac gaactacct aaaccgaga ttaaccgaa gaaccggaa ccagaaata atggcttta aaaggagaa aatattacg ttaagttgc acagcgatg tcaagttca aacagccct atgactttc cagtggaaa aaagacaac gtcgatata attaacatg aacattaac gaaaattcc agttccgat ggtaaaact acagaagca gtatccaaa ctcaacatt tacagagtg cagcattcc aatgctggg aagtaccag tgtgttgtg tcgaacatt tttggcacc acgtactcg caaaagtcg agcatttcg gttctaatt ttcccgacg tttctcaag cgacctgat aacgtaacg gtaaaagcc ggcgacgtc gccaaatta gtctgcgcg gctcacggc gagccacaa ccggaaatc gcctggcag aaagatggc ggaaatgat tttccggcg gccagagaa agacgcatg catgtgttg ccagccgat gatagtttt ttcatctca aatgtcaaa ccaacggac atgggaata tacagttgc actgcacac aacgccgct ggtactata gtcacaaac gcctcgttg acggtttat gaaaaacct tcttttgtc aaatcgatg gagaataag gaagtgacc gctggcgaa ttggtggtg cttcaatgt atggccagt gggacccca aagcccacg atcacgtgg tacaaaaat ggggagccc attcgggca actgagcgt cattttttc accgcggag gaacaactg atgataatt gtggacact gtccaatcg gattccggc aaatacgag tgtcatttg aataattct ttgggcgag aagaccgga tatagtcta cttagagtc aagcctggc aacttcacg acgactgac atgatgggt attataata ataacggtg gtatgttgc gccgtcgcc acttccatc atctgggtg gtgatcatt taccaaacg cgtcggcgg atgccgccg ccggccgcg ccgcccctc gagggcccc ctggagctg gaccgcggc gtgcaattc accgacaac gcctccgac cggtcatcg tgccgcgac agcggaacg ggcgattcc gcccgtcgc agcaacgac gacctggcg cacgaagac tacaaactg ataattaac gaggagccg caacctggc tggaatgtg atgcgaatc gccagtctg acttacatc cccgccgac cccaacagc tcgcatgcg ccgctcctt ccaggcacg ccgtacgtc cgctccacg aaccacgac cgacctgaa acgccagac aagggggcg ccgccagca gaacaaagc gaaaccacg gattga
- >TC001607 RA
gcataattg gctcatgcg gctttccaa taaatggaa tgtcgcttc agtggccga ttaaatggc ttctgtgtg tatattact gcgtcagta tgttgcaaa tttaacgat tttaataaa ttaaacaac gccccggtt gtaaccgac gaaataata gtgcagtga gatgttgaa aaacgttaa ttccataaa aatgttgcc gaaatgtat taaaatata caaatttcg gtcaaacta ctatggata attgagtgt tttttcaaa atggtgggt gtttgttat ttggctacg atcattatt tacatcatt agttcggca agttgtacg aaaagcaaa gattgtcca gaaatttgc acttgtttg ggttcttac gtcgactgt tcgtcgaaa agattgcaa gtaattcca tcgaatctt ccaaaatgg accactcac ctcgattta cgtaataac accattaaa aaacttaac gacgttact tggagaaac ctgactatc ttaactgaa ctcatattg aacaaaaac gaaatttct gttattccc aacgacgcg ttcagtaac cagaagcag ctaaaaatt cttgaactt aaccgaaat cagctgaag tccattgaa gctttaact ttcaagtca ttagaacgt ctatatgta ttaaaattg aaacgaaac caaataacg caactcaaa gatggggcc ttctacgga ttgttgtct attgacaaa ttgattttg gattacaat cacatatta gtcatttcg aaaggatgg ctttatggt ttacagtct ctaaaagaa ttgtctttg aaccacaat tacatcaat tttgtcgaa cctgaggct tgggagttt tgtaaaaaa ctggccctc ttagacttg tctaataac aggctagaa tcggtcgca gctaatacg ttcaaacat cttaacgat ctccaaaaa ctagtatta agtaacaat aagataaca tttattgaa gaaagagct tttagccac ttgccaaat ctgaaatac ttgcattta aataacaat aaaatctcg tggacgatt gaggatgcg aacggcgtg ttccaagga ctgggcaat ttgattaag ttttacctg gccgataat aacattaaa tcgataagt aaaaacgcc tttattgga ttgaagaac gtaacgtat ttaaatttg aacgacaat aacattacc tcaatccaa atgaacgcc ttctctgaa gtgcctttg ctttcggat ctcgttatt aacaccact tatttactt tgcgactgt aacattcgt tggttttac gaatggttg gacacaaaa cagttcaaa atccgtgcg atttgtgcc tatcccgaa tggttgcga ggccagtca ttggtcgaa ataccaaca tcgaatttt acatgtgac gaactacct aaaccgaga ttaaccgaa gaaccggaa ccagaaata atggcttta aaaggagaa aatattacg ttaagttgc acagcgatg tcaagttca aacagccct atgactttc cagtggaaa aaagacaac gtcgatata attaacatg aacattaac gaaaattcc agttccgat ggtaaaact acagaagca gtatccaaa ctcaacatt tacagagtg cagcattcc aatgctggg aagtaccag tgtgttgtg tcgaacatt tttggcacc acgtactcg caaaagtcg agcatttcg gttctaatt ttcccgacg tttctcaag cgacctgat aacgtaacg gtaaaagcc ggcgacgtc gccaaatta gtctgcgcg gctcacggc gagccacaa ccggaaatc gcctggcag aaagatggc ggaaatgat tttccggcg gccagagaa agacgcatg catgtgttg ccagccgat gatagtttt ttcatctca aatgtcaaa ccaacggac atgggaata tacagttgc actgcacac aacgccgct ggtactata gtcacaaac gcctcgttg acggtttat gaaaaacct tcttttgtc aaatcgatg gagaataag gaagtgacc gctggcgaa ttggtggtg cttcaatgt atggccagt gggacccca aagcccacg atcacgtgg tacaaaaat ggggagccc attcgggca actgagcgt cattttttc accgcggag gaacaactg atgataatt gtggacact gtccaatcg gattccggc aaatacgag tgtcatttg aataattct ttgggcgag aagaccgga tatagtcta cttagagtc aagcctggc aacttcacg acgactgac atgatgggt attataata ataacggtg gtatgttgc gccgtcgcc acttccatc atctgggtg gtgatcatt taccaaacg cgtcggcgg atgccgccg ccggccgcg ccgcccctc gagggcccc ctggagctg gaccgcggc gtgcaattc accgacaac gcctccgac cggtcatcg tgccgcgac agcggaacg ggcgattcc gcccgtcgc agcaacgac gacctggcg cacgaagac tacaaactg ataattaac gaggagccg caacctggc tggaatgtg atgcgaatc gccagtctg acttacatc cccgccgac cccaacagc tcgcatgcg ccgctcctt ccaggcacg ccgtacgtc cgctccacg aaccacgac cgacctgaa acgccagac aagggggcg ccgccagca gaacaaagc gaaaccacg gattgaacg ctgctggaa gtcaatttc ctactcaat ggtacaatt aagtgtata tactgtatt ttttagttc ctgtcttag attaatcgc gtttattta atgtttttc gactgttgc cgagttatt tattaagtt atttatgtt cggagttac ataacgacg tcgaactga tttcacacg agtcgtcgt gacaataac ctaagttag gtgagattt aggccaggt ttcacggag gcacactca acggttttg gccgttggc tgtgtctaa gtggagttt ggccgagat ttataccaa agcaagttg tgatgcggt aaatgaccg gaggcctta tctataaat aaaaatatt tttctagcg atataatta tattaaagc gcgtattag aaacgatac cagttatac aaattccat taaacacca attgtggcg tcgtatgta actttggat gacttgtcc cagttttat tacttcaaa ttcccgtga tattatgtt gataagtgg acataattt ttgtatatt tacaaagtt ttattagta tactgccca ttaaaagta taattcctg tagatatta ctatgttag tgcagttgt aaagtattg aaacgtgtt tgtgttgac tgtgtttat ttattaatg tgtgtaagc cgtgcgagt gtcgtcgat attgttttt attgtgtta taagcaatc tatattaac actattttg ttaatacgt tttaaattt atggtattt ttttcgata atttttaag ttctgtaat cccacttta tagtattac taagtatac ctcattgtg atttataat ttttgtcta aaataaaat aatgcctat attatataa agtggttct taggcttgc aacaaaact cactccaag ctgccggct aggccaaaa gtgggcagc ttggcgtgc cttttggtt agtccctcc cttgaaata cgcttttgt aacaaatgt gcaactcgg aagtattgg gcatgctaa tagccttat ggcacgttt tgattaaaa gtttagtcc tatt
