Status
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Gene information
General information
TC001607 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1268719..1275997
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001607
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
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    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.

    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03218

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001607 RA
      MVGVCYLAT IIIYIISSA SCTKSKDCP EICTCLGSY 
      VDCSSKRLQ VIPSNLPKW TTHLDLRNN TIKKLNDVT 
      WRNLTILTE LILNKNEIS VIPNDAFSN QKQLKILEL 
      NRNQLKSIE ALTFKSLER LYVLKLKRN QITQLKDGA 
      FYGLLSIDK LILDYNHIL VISKGWLYG LQSLKELSL 
      NHNYINFVE PEAWEFCKK LALLDLSNN RLESVAANT 
      FKHLNDLQK LVLSNNKIT FIEERAFSH LPNLKYLHL 
      NNNKISWTI EDANGVFQG LGNLIKFYL ADNNIKSIS 
      KNAFIGLKN VTYLNLNDN NITSIQMNA FSEVPLLSD 
      LVINTTYLL CDCNIRWFY EWLDTKQFK IRAICAYPE 
      WLRGQSLVE IPTSNFTCD ELPKPRLTE EPEPEIMAL 
      KGENITLSC TAMSSSNSP MTFQWKKDN VDIINMNIN 
      ENSSSDGKT TEAVSKLNI YRVQHSNAG KYQCVVSNI 
      FGTTYSQKS SISVLIFPT FLKRPDNVT VKAGDVAKL 
      VCAAHGEPQ PEIAWQKDG GNDFPAARE RRMHVLPAD 
      DSFFISNVK PTDMGIYSC TAHNAAGTI VTNASLTVY 
      EKPSFVKSM ENKEVTAGE LVVLQCMAS GTPKPTITW 
      YKNGEPIRA TERHFFTAE EQLMIIVDT VQSDSGKYE 
      CHLNNSLGE KTGYSLLRV KPGNFTTTD MMGIIIITV 
      VCCAVATSI IWVVIIYQT RRRMPPPAA PPLEGPLEL 
      DRGVQFTDN ASDRSSCRD SGTGDSARR SNDDLAHED 
      YKLIINEEP QPGWNVMRI ASLTYIPAD PNSSHAPLL 
      PGTPYVRST NHDRPETPD KGAPPAEQS ETTD
    CDS sequence
    • >TC001607 RA
      atggtgggt gtttgttat ttggctacg atcattatt 
      tacatcatt agttcggca agttgtacg aaaagcaaa 
      gattgtcca gaaatttgc acttgtttg ggttcttac 
      gtcgactgt tcgtcgaaa agattgcaa gtaattcca 
      tcgaatctt ccaaaatgg accactcac ctcgattta 
      cgtaataac accattaaa aaacttaac gacgttact 
      tggagaaac ctgactatc ttaactgaa ctcatattg 
      aacaaaaac gaaatttct gttattccc aacgacgcg 
      ttcagtaac cagaagcag ctaaaaatt cttgaactt 
      aaccgaaat cagctgaag tccattgaa gctttaact 
      ttcaagtca ttagaacgt ctatatgta ttaaaattg 
      aaacgaaac caaataacg caactcaaa gatggggcc 
      ttctacgga ttgttgtct attgacaaa ttgattttg 
      gattacaat cacatatta gtcatttcg aaaggatgg 
      ctttatggt ttacagtct ctaaaagaa ttgtctttg 
      aaccacaat tacatcaat tttgtcgaa cctgaggct 
      tgggagttt tgtaaaaaa ctggccctc ttagacttg 
      tctaataac aggctagaa tcggtcgca gctaatacg 
      ttcaaacat cttaacgat ctccaaaaa ctagtatta 
      agtaacaat aagataaca tttattgaa gaaagagct 
      tttagccac ttgccaaat ctgaaatac ttgcattta 
      aataacaat aaaatctcg tggacgatt gaggatgcg 
      aacggcgtg ttccaagga ctgggcaat ttgattaag 
      ttttacctg gccgataat aacattaaa tcgataagt 
      aaaaacgcc tttattgga ttgaagaac gtaacgtat 
      ttaaatttg aacgacaat aacattacc tcaatccaa 
      atgaacgcc ttctctgaa gtgcctttg ctttcggat 
      ctcgttatt aacaccact tatttactt tgcgactgt 
      aacattcgt tggttttac gaatggttg gacacaaaa 
      cagttcaaa atccgtgcg atttgtgcc tatcccgaa 
      tggttgcga ggccagtca ttggtcgaa ataccaaca 
      tcgaatttt acatgtgac gaactacct aaaccgaga 
      ttaaccgaa gaaccggaa ccagaaata atggcttta 
      aaaggagaa aatattacg ttaagttgc acagcgatg 
      tcaagttca aacagccct atgactttc cagtggaaa 
      aaagacaac gtcgatata attaacatg aacattaac 
      gaaaattcc agttccgat ggtaaaact acagaagca 
      gtatccaaa ctcaacatt tacagagtg cagcattcc 
      aatgctggg aagtaccag tgtgttgtg tcgaacatt 
      tttggcacc acgtactcg caaaagtcg agcatttcg 
      gttctaatt ttcccgacg tttctcaag cgacctgat 
      aacgtaacg gtaaaagcc ggcgacgtc gccaaatta 
      gtctgcgcg gctcacggc gagccacaa ccggaaatc 
      gcctggcag aaagatggc ggaaatgat tttccggcg 
      gccagagaa agacgcatg catgtgttg ccagccgat 
      gatagtttt ttcatctca aatgtcaaa ccaacggac 
      atgggaata tacagttgc actgcacac aacgccgct 
      ggtactata gtcacaaac gcctcgttg acggtttat 
      gaaaaacct tcttttgtc aaatcgatg gagaataag 
      gaagtgacc gctggcgaa ttggtggtg cttcaatgt 
      atggccagt gggacccca aagcccacg atcacgtgg 
      tacaaaaat ggggagccc attcgggca actgagcgt 
      cattttttc accgcggag gaacaactg atgataatt 
      gtggacact gtccaatcg gattccggc aaatacgag 
      tgtcatttg aataattct ttgggcgag aagaccgga 
      tatagtcta cttagagtc aagcctggc aacttcacg 
      acgactgac atgatgggt attataata ataacggtg 
      gtatgttgc gccgtcgcc acttccatc atctgggtg 
      gtgatcatt taccaaacg cgtcggcgg atgccgccg 
      ccggccgcg ccgcccctc gagggcccc ctggagctg 
      gaccgcggc gtgcaattc accgacaac gcctccgac 
      cggtcatcg tgccgcgac agcggaacg ggcgattcc 
      gcccgtcgc agcaacgac gacctggcg cacgaagac 
      tacaaactg ataattaac gaggagccg caacctggc 
      tggaatgtg atgcgaatc gccagtctg acttacatc 
      cccgccgac cccaacagc tcgcatgcg ccgctcctt 
      ccaggcacg ccgtacgtc cgctccacg aaccacgac 
      cgacctgaa acgccagac aagggggcg ccgccagca 
      gaacaaagc gaaaccacg gattga
    mRNA sequence
    • >TC001607 RA
      gcataattg gctcatgcg gctttccaa taaatggaa 
      tgtcgcttc agtggccga ttaaatggc ttctgtgtg 
      tatattact gcgtcagta tgttgcaaa tttaacgat 
      tttaataaa ttaaacaac gccccggtt gtaaccgac 
      gaaataata gtgcagtga gatgttgaa aaacgttaa 
      ttccataaa aatgttgcc gaaatgtat taaaatata 
      caaatttcg gtcaaacta ctatggata attgagtgt 
      tttttcaaa atggtgggt gtttgttat ttggctacg 
      atcattatt tacatcatt agttcggca agttgtacg 
      aaaagcaaa gattgtcca gaaatttgc acttgtttg 
      ggttcttac gtcgactgt tcgtcgaaa agattgcaa 
      gtaattcca tcgaatctt ccaaaatgg accactcac 
      ctcgattta cgtaataac accattaaa aaacttaac 
      gacgttact tggagaaac ctgactatc ttaactgaa 
      ctcatattg aacaaaaac gaaatttct gttattccc 
      aacgacgcg ttcagtaac cagaagcag ctaaaaatt 
      cttgaactt aaccgaaat cagctgaag tccattgaa 
      gctttaact ttcaagtca ttagaacgt ctatatgta 
      ttaaaattg aaacgaaac caaataacg caactcaaa 
      gatggggcc ttctacgga ttgttgtct attgacaaa 
      ttgattttg gattacaat cacatatta gtcatttcg 
      aaaggatgg ctttatggt ttacagtct ctaaaagaa 
      ttgtctttg aaccacaat tacatcaat tttgtcgaa 
      cctgaggct tgggagttt tgtaaaaaa ctggccctc 
      ttagacttg tctaataac aggctagaa tcggtcgca 
      gctaatacg ttcaaacat cttaacgat ctccaaaaa 
      ctagtatta agtaacaat aagataaca tttattgaa 
      gaaagagct tttagccac ttgccaaat ctgaaatac 
      ttgcattta aataacaat aaaatctcg tggacgatt 
      gaggatgcg aacggcgtg ttccaagga ctgggcaat 
      ttgattaag ttttacctg gccgataat aacattaaa 
      tcgataagt aaaaacgcc tttattgga ttgaagaac 
      gtaacgtat ttaaatttg aacgacaat aacattacc 
      tcaatccaa atgaacgcc ttctctgaa gtgcctttg 
      ctttcggat ctcgttatt aacaccact tatttactt 
      tgcgactgt aacattcgt tggttttac gaatggttg 
      gacacaaaa cagttcaaa atccgtgcg atttgtgcc 
      tatcccgaa tggttgcga ggccagtca ttggtcgaa 
      ataccaaca tcgaatttt acatgtgac gaactacct 
      aaaccgaga ttaaccgaa gaaccggaa ccagaaata 
      atggcttta aaaggagaa aatattacg ttaagttgc 
      acagcgatg tcaagttca aacagccct atgactttc 
      cagtggaaa aaagacaac gtcgatata attaacatg 
      aacattaac gaaaattcc agttccgat ggtaaaact 
      acagaagca gtatccaaa ctcaacatt tacagagtg 
      cagcattcc aatgctggg aagtaccag tgtgttgtg 
      tcgaacatt tttggcacc acgtactcg caaaagtcg 
      agcatttcg gttctaatt ttcccgacg tttctcaag 
      cgacctgat aacgtaacg gtaaaagcc ggcgacgtc 
      gccaaatta gtctgcgcg gctcacggc gagccacaa 
      ccggaaatc gcctggcag aaagatggc ggaaatgat 
      tttccggcg gccagagaa agacgcatg catgtgttg 
      ccagccgat gatagtttt ttcatctca aatgtcaaa 
      ccaacggac atgggaata tacagttgc actgcacac 
      aacgccgct ggtactata gtcacaaac gcctcgttg 
      acggtttat gaaaaacct tcttttgtc aaatcgatg 
      gagaataag gaagtgacc gctggcgaa ttggtggtg 
      cttcaatgt atggccagt gggacccca aagcccacg 
      atcacgtgg tacaaaaat ggggagccc attcgggca 
      actgagcgt cattttttc accgcggag gaacaactg 
      atgataatt gtggacact gtccaatcg gattccggc 
      aaatacgag tgtcatttg aataattct ttgggcgag 
      aagaccgga tatagtcta cttagagtc aagcctggc 
      aacttcacg acgactgac atgatgggt attataata 
      ataacggtg gtatgttgc gccgtcgcc acttccatc 
      atctgggtg gtgatcatt taccaaacg cgtcggcgg 
      atgccgccg ccggccgcg ccgcccctc gagggcccc 
      ctggagctg gaccgcggc gtgcaattc accgacaac 
      gcctccgac cggtcatcg tgccgcgac agcggaacg 
      ggcgattcc gcccgtcgc agcaacgac gacctggcg 
      cacgaagac tacaaactg ataattaac gaggagccg 
      caacctggc tggaatgtg atgcgaatc gccagtctg 
      acttacatc cccgccgac cccaacagc tcgcatgcg 
      ccgctcctt ccaggcacg ccgtacgtc cgctccacg 
      aaccacgac cgacctgaa acgccagac aagggggcg 
      ccgccagca gaacaaagc gaaaccacg gattgaacg 
      ctgctggaa gtcaatttc ctactcaat ggtacaatt 
      aagtgtata tactgtatt ttttagttc ctgtcttag 
      attaatcgc gtttattta atgtttttc gactgttgc 
      cgagttatt tattaagtt atttatgtt cggagttac 
      ataacgacg tcgaactga tttcacacg agtcgtcgt 
      gacaataac ctaagttag gtgagattt aggccaggt 
      ttcacggag gcacactca acggttttg gccgttggc 
      tgtgtctaa gtggagttt ggccgagat ttataccaa 
      agcaagttg tgatgcggt aaatgaccg gaggcctta 
      tctataaat aaaaatatt tttctagcg atataatta 
      tattaaagc gcgtattag aaacgatac cagttatac 
      aaattccat taaacacca attgtggcg tcgtatgta 
      actttggat gacttgtcc cagttttat tacttcaaa 
      ttcccgtga tattatgtt gataagtgg acataattt 
      ttgtatatt tacaaagtt ttattagta tactgccca 
      ttaaaagta taattcctg tagatatta ctatgttag 
      tgcagttgt aaagtattg aaacgtgtt tgtgttgac 
      tgtgtttat ttattaatg tgtgtaagc cgtgcgagt 
      gtcgtcgat attgttttt attgtgtta taagcaatc 
      tatattaac actattttg ttaatacgt tttaaattt 
      atggtattt ttttcgata atttttaag ttctgtaat 
      cccacttta tagtattac taagtatac ctcattgtg 
      atttataat ttttgtcta aaataaaat aatgcctat 
      attatataa agtggttct taggcttgc aacaaaact 
      cactccaag ctgccggct aggccaaaa gtgggcagc 
      ttggcgtgc cttttggtt agtccctcc cttgaaata 
      cgcttttgt aacaaatgt gcaactcgg aagtattgg 
      gcatgctaa tagccttat ggcacgttt tgattaaaa 
      gtttagtcc tatt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY