Status
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Gene information
General information
TC001607 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1268719..1275997
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    		TC001607
    iBeetle screen iB_03218

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection

    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection

    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001607 RA
      MVGVCYLAT IIIYIISSA SCTKSKDCP EICTCLGSY 
VDCSSKRLQ VIPSNLPKW TTHLDLRNN TIKKLNDVT 
WRNLTILTE LILNKNEIS VIPNDAFSN QKQLKILEL 
NRNQLKSIE ALTFKSLER LYVLKLKRN QITQLKDGA 
FYGLLSIDK LILDYNHIL VISKGWLYG LQSLKELSL 
NHNYINFVE PEAWEFCKK LALLDLSNN RLESVAANT 
FKHLNDLQK LVLSNNKIT FIEERAFSH LPNLKYLHL 
NNNKISWTI EDANGVFQG LGNLIKFYL ADNNIKSIS 
KNAFIGLKN VTYLNLNDN NITSIQMNA FSEVPLLSD 
LVINTTYLL CDCNIRWFY EWLDTKQFK IRAICAYPE 
WLRGQSLVE IPTSNFTCD ELPKPRLTE EPEPEIMAL 
KGENITLSC TAMSSSNSP MTFQWKKDN VDIINMNIN 
ENSSSDGKT TEAVSKLNI YRVQHSNAG KYQCVVSNI 
FGTTYSQKS SISVLIFPT FLKRPDNVT VKAGDVAKL 
VCAAHGEPQ PEIAWQKDG GNDFPAARE RRMHVLPAD 
DSFFISNVK PTDMGIYSC TAHNAAGTI VTNASLTVY 
EKPSFVKSM ENKEVTAGE LVVLQCMAS GTPKPTITW 
YKNGEPIRA TERHFFTAE EQLMIIVDT VQSDSGKYE 
CHLNNSLGE KTGYSLLRV KPGNFTTTD MMGIIIITV 
VCCAVATSI IWVVIIYQT RRRMPPPAA PPLEGPLEL 
DRGVQFTDN ASDRSSCRD SGTGDSARR SNDDLAHED 
YKLIINEEP QPGWNVMRI ASLTYIPAD PNSSHAPLL 
PGTPYVRST NHDRPETPD KGAPPAEQS ETTD
    CDS sequence
    • >TC001607 RA
      atggtgggt gtttgttat ttggctacg atcattatt 
tacatcatt agttcggca agttgtacg aaaagcaaa 
gattgtcca gaaatttgc acttgtttg ggttcttac 
gtcgactgt tcgtcgaaa agattgcaa gtaattcca 
tcgaatctt ccaaaatgg accactcac ctcgattta 
cgtaataac accattaaa aaacttaac gacgttact 
tggagaaac ctgactatc ttaactgaa ctcatattg 
aacaaaaac gaaatttct gttattccc aacgacgcg 
ttcagtaac cagaagcag ctaaaaatt cttgaactt 
aaccgaaat cagctgaag tccattgaa gctttaact 
ttcaagtca ttagaacgt ctatatgta ttaaaattg 
aaacgaaac caaataacg caactcaaa gatggggcc 
ttctacgga ttgttgtct attgacaaa ttgattttg 
gattacaat cacatatta gtcatttcg aaaggatgg 
ctttatggt ttacagtct ctaaaagaa ttgtctttg 
aaccacaat tacatcaat tttgtcgaa cctgaggct 
tgggagttt tgtaaaaaa ctggccctc ttagacttg 
tctaataac aggctagaa tcggtcgca gctaatacg 
ttcaaacat cttaacgat ctccaaaaa ctagtatta 
agtaacaat aagataaca tttattgaa gaaagagct 
tttagccac ttgccaaat ctgaaatac ttgcattta 
aataacaat aaaatctcg tggacgatt gaggatgcg 
aacggcgtg ttccaagga ctgggcaat ttgattaag 
ttttacctg gccgataat aacattaaa tcgataagt 
aaaaacgcc tttattgga ttgaagaac gtaacgtat 
ttaaatttg aacgacaat aacattacc tcaatccaa 
atgaacgcc ttctctgaa gtgcctttg ctttcggat 
ctcgttatt aacaccact tatttactt tgcgactgt 
aacattcgt tggttttac gaatggttg gacacaaaa 
cagttcaaa atccgtgcg atttgtgcc tatcccgaa 
tggttgcga ggccagtca ttggtcgaa ataccaaca 
tcgaatttt acatgtgac gaactacct aaaccgaga 
ttaaccgaa gaaccggaa ccagaaata atggcttta 
aaaggagaa aatattacg ttaagttgc acagcgatg 
tcaagttca aacagccct atgactttc cagtggaaa 
aaagacaac gtcgatata attaacatg aacattaac 
gaaaattcc agttccgat ggtaaaact acagaagca 
gtatccaaa ctcaacatt tacagagtg cagcattcc 
aatgctggg aagtaccag tgtgttgtg tcgaacatt 
tttggcacc acgtactcg caaaagtcg agcatttcg 
gttctaatt ttcccgacg tttctcaag cgacctgat 
aacgtaacg gtaaaagcc ggcgacgtc gccaaatta 
gtctgcgcg gctcacggc gagccacaa ccggaaatc 
gcctggcag aaagatggc ggaaatgat tttccggcg 
gccagagaa agacgcatg catgtgttg ccagccgat 
gatagtttt ttcatctca aatgtcaaa ccaacggac 
atgggaata tacagttgc actgcacac aacgccgct 
ggtactata gtcacaaac gcctcgttg acggtttat 
gaaaaacct tcttttgtc aaatcgatg gagaataag 
gaagtgacc gctggcgaa ttggtggtg cttcaatgt 
atggccagt gggacccca aagcccacg atcacgtgg 
tacaaaaat ggggagccc attcgggca actgagcgt 
cattttttc accgcggag gaacaactg atgataatt 
gtggacact gtccaatcg gattccggc aaatacgag 
tgtcatttg aataattct ttgggcgag aagaccgga 
tatagtcta cttagagtc aagcctggc aacttcacg 
acgactgac atgatgggt attataata ataacggtg 
gtatgttgc gccgtcgcc acttccatc atctgggtg 
gtgatcatt taccaaacg cgtcggcgg atgccgccg 
ccggccgcg ccgcccctc gagggcccc ctggagctg 
gaccgcggc gtgcaattc accgacaac gcctccgac 
cggtcatcg tgccgcgac agcggaacg ggcgattcc 
gcccgtcgc agcaacgac gacctggcg cacgaagac 
tacaaactg ataattaac gaggagccg caacctggc 
tggaatgtg atgcgaatc gccagtctg acttacatc 
cccgccgac cccaacagc tcgcatgcg ccgctcctt 
ccaggcacg ccgtacgtc cgctccacg aaccacgac 
cgacctgaa acgccagac aagggggcg ccgccagca 
gaacaaagc gaaaccacg gattga
    mRNA sequence
    • >TC001607 RA
      gcataattg gctcatgcg gctttccaa taaatggaa 
tgtcgcttc agtggccga ttaaatggc ttctgtgtg 
tatattact gcgtcagta tgttgcaaa tttaacgat 
tttaataaa ttaaacaac gccccggtt gtaaccgac 
gaaataata gtgcagtga gatgttgaa aaacgttaa 
ttccataaa aatgttgcc gaaatgtat taaaatata 
caaatttcg gtcaaacta ctatggata attgagtgt 
tttttcaaa atggtgggt gtttgttat ttggctacg 
atcattatt tacatcatt agttcggca agttgtacg 
aaaagcaaa gattgtcca gaaatttgc acttgtttg 
ggttcttac gtcgactgt tcgtcgaaa agattgcaa 
gtaattcca tcgaatctt ccaaaatgg accactcac 
ctcgattta cgtaataac accattaaa aaacttaac 
gacgttact tggagaaac ctgactatc ttaactgaa 
ctcatattg aacaaaaac gaaatttct gttattccc 
aacgacgcg ttcagtaac cagaagcag ctaaaaatt 
cttgaactt aaccgaaat cagctgaag tccattgaa 
gctttaact ttcaagtca ttagaacgt ctatatgta 
ttaaaattg aaacgaaac caaataacg caactcaaa 
gatggggcc ttctacgga ttgttgtct attgacaaa 
ttgattttg gattacaat cacatatta gtcatttcg 
aaaggatgg ctttatggt ttacagtct ctaaaagaa 
ttgtctttg aaccacaat tacatcaat tttgtcgaa 
cctgaggct tgggagttt tgtaaaaaa ctggccctc 
ttagacttg tctaataac aggctagaa tcggtcgca 
gctaatacg ttcaaacat cttaacgat ctccaaaaa 
ctagtatta agtaacaat aagataaca tttattgaa 
gaaagagct tttagccac ttgccaaat ctgaaatac 
ttgcattta aataacaat aaaatctcg tggacgatt 
gaggatgcg aacggcgtg ttccaagga ctgggcaat 
ttgattaag ttttacctg gccgataat aacattaaa 
tcgataagt aaaaacgcc tttattgga ttgaagaac 
gtaacgtat ttaaatttg aacgacaat aacattacc 
tcaatccaa atgaacgcc ttctctgaa gtgcctttg 
ctttcggat ctcgttatt aacaccact tatttactt 
tgcgactgt aacattcgt tggttttac gaatggttg 
gacacaaaa cagttcaaa atccgtgcg atttgtgcc 
tatcccgaa tggttgcga ggccagtca ttggtcgaa 
ataccaaca tcgaatttt acatgtgac gaactacct 
aaaccgaga ttaaccgaa gaaccggaa ccagaaata 
atggcttta aaaggagaa aatattacg ttaagttgc 
acagcgatg tcaagttca aacagccct atgactttc 
cagtggaaa aaagacaac gtcgatata attaacatg 
aacattaac gaaaattcc agttccgat ggtaaaact 
acagaagca gtatccaaa ctcaacatt tacagagtg 
cagcattcc aatgctggg aagtaccag tgtgttgtg 
tcgaacatt tttggcacc acgtactcg caaaagtcg 
agcatttcg gttctaatt ttcccgacg tttctcaag 
cgacctgat aacgtaacg gtaaaagcc ggcgacgtc 
gccaaatta gtctgcgcg gctcacggc gagccacaa 
ccggaaatc gcctggcag aaagatggc ggaaatgat 
tttccggcg gccagagaa agacgcatg catgtgttg 
ccagccgat gatagtttt ttcatctca aatgtcaaa 
ccaacggac atgggaata tacagttgc actgcacac 
aacgccgct ggtactata gtcacaaac gcctcgttg 
acggtttat gaaaaacct tcttttgtc aaatcgatg 
gagaataag gaagtgacc gctggcgaa ttggtggtg 
cttcaatgt atggccagt gggacccca aagcccacg 
atcacgtgg tacaaaaat ggggagccc attcgggca 
actgagcgt cattttttc accgcggag gaacaactg 
atgataatt gtggacact gtccaatcg gattccggc 
aaatacgag tgtcatttg aataattct ttgggcgag 
aagaccgga tatagtcta cttagagtc aagcctggc 
aacttcacg acgactgac atgatgggt attataata 
ataacggtg gtatgttgc gccgtcgcc acttccatc 
atctgggtg gtgatcatt taccaaacg cgtcggcgg 
atgccgccg ccggccgcg ccgcccctc gagggcccc 
ctggagctg gaccgcggc gtgcaattc accgacaac 
gcctccgac cggtcatcg tgccgcgac agcggaacg 
ggcgattcc gcccgtcgc agcaacgac gacctggcg 
cacgaagac tacaaactg ataattaac gaggagccg 
caacctggc tggaatgtg atgcgaatc gccagtctg 
acttacatc cccgccgac cccaacagc tcgcatgcg 
ccgctcctt ccaggcacg ccgtacgtc cgctccacg 
aaccacgac cgacctgaa acgccagac aagggggcg 
ccgccagca gaacaaagc gaaaccacg gattgaacg 
ctgctggaa gtcaatttc ctactcaat ggtacaatt 
aagtgtata tactgtatt ttttagttc ctgtcttag 
attaatcgc gtttattta atgtttttc gactgttgc 
cgagttatt tattaagtt atttatgtt cggagttac 
ataacgacg tcgaactga tttcacacg agtcgtcgt 
gacaataac ctaagttag gtgagattt aggccaggt 
ttcacggag gcacactca acggttttg gccgttggc 
tgtgtctaa gtggagttt ggccgagat ttataccaa 
agcaagttg tgatgcggt aaatgaccg gaggcctta 
tctataaat aaaaatatt tttctagcg atataatta 
tattaaagc gcgtattag aaacgatac cagttatac 
aaattccat taaacacca attgtggcg tcgtatgta 
actttggat gacttgtcc cagttttat tacttcaaa 
ttcccgtga tattatgtt gataagtgg acataattt 
ttgtatatt tacaaagtt ttattagta tactgccca 
ttaaaagta taattcctg tagatatta ctatgttag 
tgcagttgt aaagtattg aaacgtgtt tgtgttgac 
tgtgtttat ttattaatg tgtgtaagc cgtgcgagt 
gtcgtcgat attgttttt attgtgtta taagcaatc 
tatattaac actattttg ttaatacgt tttaaattt 
atggtattt ttttcgata atttttaag ttctgtaat 
cccacttta tagtattac taagtatac ctcattgtg 
atttataat ttttgtcta aaataaaat aatgcctat 
attatataa agtggttct taggcttgc aacaaaact 
cactccaag ctgccggct aggccaaaa gtgggcagc 
ttggcgtgc cttttggtt agtccctcc cttgaaata 
cgcttttgt aacaaatgt gcaactcgg aagtattgg 
gcatgctaa tagccttat ggcacgttt tgattaaaa 
gtttagtcc tatt
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