Status
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Gene information
General information
TC001601 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007416.3: 1205179..1210999
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001601
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
    {{ goGene }}
    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
    {{ blurEvidenceResult }}
    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
    {{ blurReferenceResult }}
    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
    You may enter your name. The contact information can help us to clarify any questions. If you agree, we may show your name on this page to honor your contribution.
    Email
    We will use your email address only for possible questions regarding this annotation. We will never show your address or give it away.
    Publish name
    If you have entered your name and agree by marking the checkbox. we might show your name on this page to honor your contribution.
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03217

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (11 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001601 RA
      MNVSDIFEE IVPKCSEEA EPPADPEIV DSTQTNPSD 
      DNSKETPDD APTNPELFD TLMDTSDQT PVEKDDNND 
      KLDSTSAPL SCQTPEDNT IPEVIESDN LDSCDKLED 
      EANKIGEMT ADVIKEIEK INEKMDIDN PDESTVIEE 
      ENKDANTSE ESEKTFEEE EPKDDTVHE ESQDKQSED 
      EADKTVENE EPREEYEYV GLDEQPVLD KSNTEDKDN 
      ENAEGNTEN EDDEQRDNP LFVNIDKED NDKETNNKD 
      ELLEEDPIA SDGFIPENQ EGADEELCI IPDTERVIS 
      QEEKEAAFS IPPLRDISE ASSSNEVPE DGATPEPPA 
      ASTDASSST NKFKISKCS NEELQTCLQ CVLKRKVKY 
      TVTKDDDVQ YICHDTCLD NFKVDNANN YVLTWEGEI 
      RVKDLATNR EPPKLSFER KCVVCKKKT DDEENNLTW 
      EIMDFCNEF CLSKYQKEI GSSCASCNA EVRPNSLGK 
      YCVRFGNDI RQFCSSHCL EEYKKGLKV CSYCQQDMS 
      TETDGFLAP VGDKGQFKD FCSQKCMEK YDIMTNNRP 
      PTVDEGTKC SVCKTDNPI TIEYETDGA PNYFCSDRC 
      FVAFSFVNN IAPGKCSMC RRYFPNEIL EKNTMFYDN 
      VQYSFCSNS CQNIYIIAH RKIVPCAWC KVKKYNFDM 
      IRRYFKTGP VLNMCSINC LSLFQVSVN AVHSKKTLC 
      NFCNKRHQP MYHLTMSDA TIRNFCSYS CVMSFQNKF 
      KKSPITLNT EDLSYPVPA GEPKKNRKF STELAKPVS 
      PPLPVISSV QSLAASLAT KNGNYTGNR DNKRAKTNS 
      NQQHILNSN VEPPVKVKH HIIIKSSPT PDQRNVATM 
      CKLRQHHKS TSIKPNMTD KAMQTDQRE AEKVLIPVP 
      IPVYIPSPL PMYSVPAPY PLPFPLPIP VPIFIPTTR 
      NSAKGIMKE IKKIQVKIP TDPYEAELL MMAEMVAGD 
      KKEENTDSE SDVDEAGPG DEGTFSPEP VDASNTFGD 
      DMLQMALKM ATELEEPAV DLEGALTAN TITAPQGSE 
      TTTEETVDD AQPILQINE RQSFRGRKR GGRGGKGGS 
      GAKRRRMSQ PAEIPVIPP QPVAPPEPA EKPDAHLCL 
      KYTFGVNAW KQWVTTKNT ELEKSTKYA KPFKTEILQ 
      LTADELNYS LCLFVKEVR KPNGAEYAP DTIYYLCLG 
      IQQYLFENG RIDNIFCDP YYEQFTDSL DEVARKFSV 
      LYNDSHYIV TRVEEEHLW ESKQLGAHS PHVLLSTLM 
      FFNTKHFNL TSVQEHMQL SFSHIMKHW KRNPNQPGV 
      TRVPGSRNV LLRFYPPQT AIQNNTRKK KVYEQQEND 
      ENPLRCPVK LYEFYLSKC PESVKTRND VFYLQPERS 
      CVPDSPVWY STTALPQEA LEKMLHRVK MVKEINVAL 
      LTS
    CDS sequence
    • >TC001601 RA
      atgaacgtc agtgacatt tttgaggag attgtcccg 
      aaatgtagc gaggaggca gagccgcca gctgacccg 
      gaaattgtt gattccact cagactaat ccaagtgat 
      gataactcc aaagaaacc ccagacgat gcacctaca 
      aacccagaa ctctttgac acccttatg gacacttct 
      gatcaaact ccggttgag aaagacgat aataatgac 
      aaattggac tcaacgtca gctccgctc agttgtcaa 
      actcctgaa gataatact atacctgaa gtcattgaa 
      tcggataat ttagatagc tgtgataag ttggaagac 
      gaagcgaat aaaatcgga gaaatgaca gcggacgtg 
      atcaaagaa attgaaaaa attaacgaa aaaatggat 
      atcgataac ccagacgaa agcactgtt attgaagaa 
      gagaataaa gacgccaat acgagtgaa gagagcgag 
      aagacgttt gaagaagag gagcccaaa gacgacaca 
      gttcacgag gaatctcaa gacaagcaa agtgaagac 
      gaggctgac aaaactgta gaaaatgag gagccgcgc 
      gaagaatac gagtatgtg ggactggat gagcagcca 
      gtccttgac aaaagcaac actgaagac aaagacaat 
      gaaaacgcg gaaggaaac accgaaaat gaagacgac 
      gagcagaga gacaacccc ttattcgtc aacattgat 
      aaagaagac aacgataaa gagacaaac aacaaggat 
      gagttgctg gaggaagac cccatcgcg tccgatggc 
      ttcattcca gaaaatcag gaaggggcc gatgaggag 
      ctgtgcata atccccgac actgaacga gtcatatca 
      caggaggag aaagaagca gctttctcg atcccgccc 
      ttaagagac atatcagaa gcgagctct tctaacgaa 
      gttcccgag gatggcgcc actcctgag ccgccggca 
      gcatccacc gacgcgtct tcctccacc aataagttt 
      aaaatatca aagtgcagc aatgaggag ctccagact 
      tgtcttcaa tgtgttttg aaacgcaaa gtcaaatac 
      accgttact aaagacgac gacgtccag tatatttgc 
      cacgacact tgcttagac aatttcaaa gtagacaac 
      gcgaataat tacgtgttg acatgggag ggcgagatc 
      cgcgtgaaa gacttggcg actaatcga gagcctcct 
      aagttgtcc tttgagcgc aagtgcgtc gtttgtaaa 
      aagaaaacc gacgatgag gagaacaat ttgacttgg 
      gaaatcatg gatttttgt aacgagttt tgtctatct 
      aagtaccaa aaggaaatc gggtcgagt tgcgcgagt 
      tgtaacgcc gaagttcgg ccgaattct ctcgggaaa 
      tattgcgtt cgtttcggc aatgacatt aggcagttt 
      tgctcaagt cactgcctt gaggagtat aaaaagggc 
      ctgaaggtc tgctcctac tgccagcaa gacatgtcg 
      acggaaacg gacggcttt ctagccccc gtgggcgac 
      aaaggccag tttaaagat ttctgttcg caaaaatgt 
      atggaaaag tacgacatt atgaccaat aataggcca 
      cccacagtc gacgagggc accaagtgt tcggtctgc 
      aaaactgac aatccgatc acgattgaa tatgagact 
      gatggagcg ccgaattat ttttgtagc gataggtgt 
      tttgtcgct ttcagtttc gttaacaat atcgcgccg 
      gggaaatgt tccatgtgc cgcagatac ttccctaat 
      gaaattcta gaaaaaaat accatgttt tatgataat 
      gttcagtat agtttttgc tcgaatagc tgtcagaat 
      atttacata atagcacat cggaaaata gtcccctgt 
      gcgtggtgc aaagtcaaa aaatataat tttgatatg 
      attcggagg tacttcaag acagggcct gtactaaac 
      atgtgcagc ataaattgt ctcagcttg ttccaagtg 
      tccgtaaac gctgtacat tcgaaaaaa accctttgt 
      aatttttgt aacaagcgg catcaaccg atgtatcac 
      cttacaatg tcggatgcc acaattcga aatttctgt 
      tcgtattcg tgtgtcatg tcgttccaa aacaagttt 
      aaaaaatcg cccattacc ctaaacact gaggatttg 
      tcgtaccct gtgcctgct ggtgagccc aaaaaaaat 
      cgaaaattt tccaccgaa cttgcaaaa ccagtctca 
      ccgccctta ccggtgatc agtagcgta cagagtttg 
      gctgcgagt ttggccacc aaaaatggg aattacaca 
      ggcaaccga gataacaaa agggcaaaa acaaactcg 
      aaccagcaa catatatta aactcaaat gttgagccc 
      cccgttaaa gtgaaacat catattatt atcaaatca 
      tcgccgaca cctgatcag cgtaatgtt gccacgatg 
      tgcaagttg agacagcac cacaaaagc accagcatt 
      aagccgaac atgacggac aaggcaatg cagacagac 
      caacgcgag gccgaaaaa gtcctaatc cctgtccca 
      atccccgtg tatatacca tcccccctc ccgatgtat 
      tcagtcccc gcgccttac ccgctccca ttcccgctc 
      cccatccca gtcccgatt ttcatccca accacccgg 
      aactcagca aagggcatc atgaaagaa atcaagaaa 
      atccaagtc aaaataccg accgatcca tacgaggct 
      gaacttctc atgatggcc gaaatggtc gccggtgac 
      aaaaaggaa gaaaacacc gactcagaa agcgacgtt 
      gacgaggcc ggccctgga gacgagggc accttcagc 
      cccgagccg gtggatgct agtaacact ttcggagac 
      gatatgcta caaatggcg ctcaaaatg gccaccgag 
      cttgaagag cccgcggtg gacctggag ggggccctc 
      acggccaac accataaca gccccccaa ggcagcgaa 
      accaccacc gaggaaaca gtcgacgat gcccaaccc 
      atcttgcaa attaacgag agacagtcg ttcaggggg 
      cgcaaacgg ggcggccga ggcgggaag gggggctcg 
      ggcgccaaa cgtcggagg atgtctcag ccggccgaa 
      atcccggtc attccccca caaccggtg gcgcccccc 
      gagcccgcc gagaaaccc gacgcgcat ctgtgcctc 
      aaatacacg tttggggtt aatgcgtgg aagcagtgg 
      gtgaccacg aaaaacacc gaactggag aaatctaca 
      aagtacgcg aaacctttc aaaacggaa attttgcaa 
      ttaactgct gatgaatta aactactcg ttgtgtctc 
      ttcgttaag gaggtgcgg aagcccaac ggggctgaa 
      tacgcccct gatacgatt tattacttg tgcttaggg 
      attcaacaa tacttattt gagaatgga cgcatcgat 
      aacattttt tgtgatcct tattacgaa cagttcact 
      gatagcttg gacgaggtg gctcgaaaa ttttcagta 
      ctctacaac gactcgcac tacatcgtt acaagagtc 
      gaggaggag cacctgtgg gagagtaaa cagctaggt 
      gcccattcc ccgcatgtc ttactgagc actttgatg 
      tttttcaac acgaaacat ttcaatctc actagtgtg 
      caagaacac atgcagttg tcgttttcc cacatcatg 
      aaacattgg aagaggaat ccgaaccaa ccaggcgtg 
      actcgagtc ccaggttcg cgtaatgtc cttttacgg 
      ttttatccg ccccaaacg gcaatacaa aacaacacg 
      cgcaagaaa aaggtgtac gagcagcag gaaaacgac 
      gagaatccc ttgcggtgc ccggtcaag ttgtacgaa 
      ttctacttg tcgaaatgt cccgaaagt gtcaaaact 
      cgcaatgat gtgttttat ttgcaaccg gaaagatcg 
      tgtgtacct gatagtcct gtttggtat tcaactacg 
      gctctgccg caagaagcg ctggagaaa atgctgcac 
      cgggttaag atggtcaag gagattaat gtcgcactt 
      ttaactagt taa
    mRNA sequence
    • >TC001601 RA
      gacatctct taaatatcc tccaaacta ccagtcaaa 
      ctgttgcag tttttatcc atggtttat ctaatttta 
      gtttgcccg ccacgggag ggaaacccc cgagatttg 
      cttcttatg tatctggcg cgtgtcggt tggcagcaa 
      tgttgttat ttctcgagc tgccaccat tattgttaa 
      tactttcgt gaaaaaatg ttagtttga tttttaatt 
      ccagcaaat cgatgtaaa tttctttaa aaatgtaat 
      atactaaag tctggatcg ttttaacta ttaattaag 
      ttgttgggt ataatttgg ctgtaaatg tgattaatt 
      ttgaggacg ccggcgttt gttaaagct agtgtagaa 
      gtgtggtgt atttaatta tttgcggat tgtttaaat 
      agtaaacaa ccgtgttag tttaagttt cttcccctt 
      tggttttta agtcgtttt taatcatga acgtcagtg 
      acatttttg aggagattg tcccgaaat gtagcgagg 
      aggcagagc cgccagctg acccggaaa ttgttgatt 
      ccactcaga ctaatccaa gtgatgata actccaaag 
      aaaccccag acgatgcac ctacaaacc cagaactct 
      ttgacaccc ttatggaca cttctgatc aaactccgg 
      ttgagaaag acgataata atgacaaat tggactcaa 
      cgtcagctc cgctcagtt gtcaaactc ctgaagata 
      atactatac ctgaagtca ttgaatcgg ataatttag 
      atagctgtg ataagttgg aagacgaag cgaataaaa 
      tcggagaaa tgacagcgg acgtgatca aagaaattg 
      aaaaaatta acgaaaaaa tggatatcg ataacccag 
      acgaaagca ctgttattg aagaagaga ataaagacg 
      ccaatacga gtgaagaga gcgagaaga cgtttgaag 
      aagaggagc ccaaagacg acacagttc acgaggaat 
      ctcaagaca agcaaagtg aagacgagg ctgacaaaa 
      ctgtagaaa atgaggagc cgcgcgaag aatacgagt 
      atgtgggac tggatgagc agccagtcc ttgacaaaa 
      gcaacactg aagacaaag acaatgaaa acgcggaag 
      gaaacaccg aaaatgaag acgacgagc agagagaca 
      accccttat tcgtcaaca ttgataaag aagacaacg 
      ataaagaga caaacaaca aggatgagt tgctggagg 
      aagacccca tcgcgtccg atggcttca ttccagaaa 
      atcaggaag gggccgatg aggagctgt gcataatcc 
      ccgacactg aacgagtca tatcacagg aggagaaag 
      aagcagctt tctcgatcc cgcccttaa gagacatat 
      cagaagcga gctcttcta acgaagttc ccgaggatg 
      gcgccactc ctgagccgc cggcagcat ccaccgacg 
      cgtcttcct ccaccaata agtttaaaa tatcaaagt 
      gcagcaatg aggagctcc agacttgtc ttcaatgtg 
      ttttgaaac gcaaagtca aatacaccg ttactaaag 
      acgacgacg tccagtata tttgccacg acacttgct 
      tagacaatt tcaaagtag acaacgcga ataattacg 
      tgttgacat gggagggcg agatccgcg tgaaagact 
      tggcgacta atcgagagc ctcctaagt tgtcctttg 
      agcgcaagt gcgtcgttt gtaaaaaga aaaccgacg 
      atgaggaga acaatttga cttgggaaa tcatggatt 
      tttgtaacg agttttgtc tatctaagt accaaaagg 
      aaatcgggt cgagttgcg cgagttgta acgccgaag 
      ttcggccga attctctcg ggaaatatt gcgttcgtt 
      tcggcaatg acattaggc agttttgct caagtcact 
      gccttgagg agtataaaa agggcctga aggtctgct 
      cctactgcc agcaagaca tgtcgacgg aaacggacg 
      gctttctag cccccgtgg gcgacaaag gccagttta 
      aagatttct gttcgcaaa aatgtatgg aaaagtacg 
      acattatga ccaataata ggccaccca cagtcgacg 
      agggcacca agtgttcgg tctgcaaaa ctgacaatc 
      cgatcacga ttgaatatg agactgatg gagcgccga 
      attattttt gtagcgata ggtgttttg tcgctttca 
      gtttcgtta acaatatcg cgccgggga aatgttcca 
      tgtgccgca gatacttcc ctaatgaaa ttctagaaa 
      aaaatacca tgttttatg ataatgttc agtatagtt 
      tttgctcga atagctgtc agaatattt acataatag 
      cacatcgga aaatagtcc cctgtgcgt ggtgcaaag 
      tcaaaaaat ataattttg atatgattc ggaggtact 
      tcaagacag ggcctgtac taaacatgt gcagcataa 
      attgtctca gcttgttcc aagtgtccg taaacgctg 
      tacattcga aaaaaaccc tttgtaatt tttgtaaca 
      agcggcatc aaccgatgt atcacctta caatgtcgg 
      atgccacaa ttcgaaatt tctgttcgt attcgtgtg 
      tcatgtcgt tccaaaaca agtttaaaa aatcgccca 
      ttaccctaa acactgagg atttgtcgt accctgtgc 
      ctgctggtg agcccaaaa aaaatcgaa aattttcca 
      ccgaacttg caaaaccag tctcaccgc ccttaccgg 
      tgatcagta gcgtacaga gtttggctg cgagtttgg 
      ccaccaaaa atgggaatt acacaggca accgagata 
      acaaaaggg caaaaacaa actcgaacc agcaacata 
      tattaaact caaatgttg agccccccg ttaaagtga 
      aacatcata ttattatca aatcatcgc cgacacctg 
      atcagcgta atgttgcca cgatgtgca agttgagac 
      agcaccaca aaagcacca gcattaagc cgaacatga 
      cggacaagg caatgcaga cagaccaac gcgaggccg 
      aaaaagtcc taatccctg tcccaatcc ccgtgtata 
      taccatccc ccctcccga tgtattcag tccccgcgc 
      cttacccgc tcccattcc cgctcccca tcccagtcc 
      cgattttca tcccaacca cccggaact cagcaaagg 
      gcatcatga aagaaatca agaaaatcc aagtcaaaa 
      taccgaccg atccatacg aggctgaac ttctcatga 
      tggccgaaa tggtcgccg gtgacaaaa aggaagaaa 
      acaccgact cagaaagcg acgttgacg aggccggcc 
      ctggagacg agggcacct tcagccccg agccggtgg 
      atgctagta acactttcg gagacgata tgctacaaa 
      tggcgctca aaatggcca ccgagcttg aagagcccg 
      cggtggacc tggaggggg ccctcacgg ccaacacca 
      taacagccc cccaaggca gcgaaacca ccaccgagg 
      aaacagtcg acgatgccc aacccatct tgcaaatta 
      acgagagac agtcgttca gggggcgca aacggggcg 
      gccgaggcg ggaaggggg gctcgggcg ccaaacgtc 
      ggaggatgt ctcagccgg ccgaaatcc cggtcattc 
      ccccacaac cggtggcgc cccccgagc ccgccgaga 
      aacccgacg cgcatctgt gcctcaaat acacgtttg 
      gggttaatg cgtggaagc agtgggtga ccacgaaaa 
      acaccgaac tggagaaat ctacaaagt acgcgaaac 
      ctttcaaaa cggaaattt tgcaattaa ctgctgatg 
      aattaaact actcgttgt gtctcttcg ttaaggagg 
      tgcggaagc ccaacgggg ctgaatacg cccctgata 
      cgatttatt acttgtgct tagggattc aacaatact 
      tatttgaga atggacgca tcgataaca ttttttgtg 
      atccttatt acgaacagt tcactgata gcttggacg 
      aggtggctc gaaaatttt cagtactct acaacgact 
      cgcactaca tcgttacaa gagtcgagg aggagcacc 
      tgtgggaga gtaaacagc taggtgccc attccccgc 
      atgtcttac tgagcactt tgatgtttt tcaacacga 
      aacatttca atctcacta gtgtgcaag aacacatgc 
      agttgtcgt tttcccaca tcatgaaac attggaaga 
      ggaatccga accaaccag gcgtgactc gagtcccag 
      gttcgcgta atgtccttt tacggtttt atccgcccc 
      aaacggcaa tacaaaaca acacgcgca agaaaaagg 
      tgtacgagc agcaggaaa acgacgaga atcccttgc 
      ggtgcccgg tcaagttgt acgaattct acttgtcga 
      aatgtcccg aaagtgtca aaactcgca atgatgtgt 
      tttatttgc aaccggaaa gatcgtgtg tacctgata 
      gtcctgttt ggtattcaa ctacggctc tgccgcaag 
      aagcgctgg agaaaatgc tgcaccggg ttaagatgg 
      tcaaggaga ttaatgtcg cacttttaa ctagttaag 
      cagttcata ttagtttgg cttattaat ttgaaggat 
      caataaatg tagattaaa atg
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY