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Gene information
General information
TC032353
Former TC numbers

If one gene is splitted or genes are merged, the original TC numbers are listed. One listed former TC number indicates the previous gene has be splitted, serveral former TC number represents a merged gene.

TC001582,TC001581
Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3068743..3081939
Sequence information
Transcripts / Proteins (2) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC032353
    iBeetle screen iB_03214

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 50.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of embryonic musculature and early development
    number of eggs/embryos/larvae on slide: > 50 embryo/egg with embryonic tissue, no cuticle, but muscle fluorescence - Penetrance (percentage of animals/eggs on slide): less than 30%

    embryo/egg with embryonic tissue, no cuticle, no fluorescence - Penetrance (percentage of animals/eggs on slide): 50% - 80%

    larval musculature pattern irregular - Penetrance (percentage of animals/eggs on slide): less than 30%

    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    remarks: muscle pattern potentially obscured by segmentation defect,
    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    embryo/egg without visible larval cuticle in cuticle preparation - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    affected eggs/embryos/larvae - Penetrance (percentage of animals/eggs on slide): 50% - 80%
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC032353 RA
      MGTAINHAA AIKRLEALF NEVKLEQKP APEVHVEVE 
      SKISTKCDV HGKNGRRGS LGNVLHITN RRDSLGNVV 
      SQKRDSNQL YAGLFGNGV GPRRESMPA LNNYGTYYR 
      RRDSLALPN GPPKKDMEH PSQFASTLR KDPLGSSMG 
      SLRKDNGRW GPRRDSLGG SMSSLKSNF LQVPNQMRR 
      NSKNYSTDS LDGRRNSWD PNRRGSSGS SCWEEPIWQ 
      SRKTIIMDD QEQISRGER EKKLVEVPP DAELMCEIL 
      HNETQPTED TTPPEEEEK PTEEEEKTE EEDAQKQPR 
      LPHWQGIDL LARKPWPSA QRINTQKSP FNFRPPEPP 
      TWREPNPYS YQAPLYNYP NPKRDHYFL SPYYRNAMP 
      PPPNPNRLL MIDPRRRFV KQNEDSCYC RSRSMEDVR 
      ADVVTPDFE NDVNGNRVP IPMRKDFSK FANRRSMDN 
      LLVDTNFSP PRKRAGRLQ TIIINWIFQ LQPVPRNSS 
      EQDNSDSNG ARPRFVAVT TLEDVQAVR CAEFHPGGQ 
      LYAVGSNSK TLRICAYPK LSDLREDHQ TYQPMVLFK 
      RTKHHKGSI YCLAWSPMG DLMATGSND KTVKLMRFN 
      ADTSNLEGE EIELSMHDG TIRDLCFLE DTSNKSSLL 
      ISGGAGDCK IYVTDCATG TPFQALSGH TGHILSLYT 
      WGGAMFVSG SHDKTVRFW DLRTRGCVN MVTPCTIPG 
      SRQGSPVAS LCVDPSGRL LVSGHEDSS CVLYDIRGG 
      RSVQCFKPH SADVRSIRF SPSAYYLLS AGYDNKLVL 
      TDLQGWYYI YFKSFRDSA SEM
    • >TC032353 RB
      MGTAINHAA AIKRLEALF NEVKLEQKP APEVHVEVE 
      SKISTKCDV HGKNGRRGS LGNVLHITN RRDSLGNVV 
      SQKRDSNQL YAGLFGNGV GPRRESMPA LNNYGTYYR 
      RRDSLALPN GPPKKDMEH PSQFASTLR KDPLGSSMG 
      SLRKDNGRW GPRRDSLGG SMSSLKSNF LQVPNQMRR 
      NSKNYSTDS LDGRRNSWD PNRRGSSGS SCWEEPIWQ 
      SRKTIIMDD QEQISRGER EKKLVEVPP DAELMCEIL 
      HNETQPTED TTPPEEEEK PTEEEEKTE EEDAVRVCV 
      LQLSEMGDL MLKQKQPRL PHWQGIDLL ARKPWPSAQ 
      RINTQKSPF NFRPPEPPT WREPNPYSY QAPLYNYPN 
      PKRDHYFLS PYYRNAMPP PPNPNRLLM IDPRRRFVK 
      QNEDSCYCR SRSMEDVRA DVVTPDFEN DVNGNRVPI 
      PMRKDFSKF ANRRSMDNL LVDTNFSPP RKRAGRLQT 
      IIINWIFQL QPVPRNSSE QDNSDSNGA RPRFVAVTT 
      LEDVQAVRC AEFHPGGQL YAVGSNSKT LRICAYPKL 
      SDLREDHQT YQPMVLFKR TKHHKGSIY CLAWSPMGD 
      LMATGSNDK TVKLMRFNA DTSNLEGEE IELSMHDGT 
      IRDLCFLED TSNKSSLLI SGGAGDCKI YVTDCATGT 
      PFQALSGHT GHILSLYTW GGAMFVSGS HDKTVRFWD 
      LRTRGCVNM VTPCTIPGS RQGSPVASL CVDPSGRLL 
      VSGHEDSSC VLYDIRGGR SVQCFKPHS ADVRSIRFS 
      PSAYYLLSA GYDNKLVLT DLQGWYYIY FKSFRDSAS 
      EM
    CDS sequence
    • >TC032353 RA
      atgggcact gccatcaac catgcagct gccattaaa 
      aggctcgag gccctcttc aacgaggtt aagctcgag 
      caaaaacca gcaccagaa gtgcacgtc gaagtggaa 
      tcgaaaata agtacaaaa tgtgatgtg cacggaaaa 
      aacggacgg cgaggttcg ctaggaaat gttctgcat 
      attacaaat cggagagat tcgctgggg aatgttgtc 
      agtcaaaag agggactcc aaccagctt tatgccgga 
      cttttcgga aacggcgtt ggtccaagg agggaatca 
      atgcctgca cttaataat tacggcact tactatagg 
      cgccgtgac tctctggcc cttccgaac ggtccgccc 
      aaaaaagac atggagcat ccaagtcag tttgcttca 
      actttacgg aaggatcct ctgggatca tcaatggga 
      tcgttacgt aaagataac ggccgttgg ggccctcgt 
      agagactcc ctgggcgga tcaatgtcc tccctaaaa 
      tcaaacttc ttacaagtg cctaatcaa atgagacgt 
      aattcgaaa aattattcc accgattcg ctcgacggg 
      cgccgaaat tcctgggac cccaaccgc agaggaagc 
      tccggaagt tcatgttgg gaagaaccg atttggcaa 
      agcagaaag actattatt atggatgac caagaacaa 
      atttcacgc ggcgaacgc gaaaaaaaa ttagttgag 
      gtaccgccc gatgcggaa ctcatgtgt gaaattctt 
      cacaacgaa acgcaacct actgaagat acgacaccg 
      cctgaagaa gaagaaaaa cccacagag gaagaagag 
      aaaacagag gaggaagat gcgcaaaag cagccgcgt 
      ctgccccat tggcaaggc atcgaccta ctggcgagg 
      aagccgtgg ccttccgcc caaaggata aacacccag 
      aagtcgccc tttaacttc cggccaccg gaaccccca 
      acctggagg gaacccaac ccttactcc taccaggcg 
      cccctttac aactacccg aacccgaaa cgggaccat 
      tacttcctc tcgccctat taccgcaat gccatgccg 
      ccgcctcct aaccccaat cggctgctg atgatcgat 
      ccgagaaga cgcttcgtt aagcagaac gaagattcg 
      tgttattgt cgatcgaga tcgatggag gacgttagg 
      gcagatgtt gttacaccg gattttgag aacgatgtc 
      aacggcaat cgggttccg attcccatg aggaaggat 
      ttcagcaaa tttgcaaac aggaggtct atggataat 
      cttttggtg gatacgaat ttttcgcca ccgaggaaa 
      cgcgccgga aggctgcag acgataata atcaattgg 
      atttttcaa ttacagcct gttccgagg aactcatca 
      gaacaagat aatagcgac agtaatggt gctagacct 
      agatttgtg gctgttaca actttggaa gatgttcaa 
      gctgtcaga tgtgcagaa tttcatccc ggcggtcaa 
      ctgtatgct gtcggttcc aattcgaaa actttacga 
      atatgtgct tatcccaaa ctcagtgac ctcagggaa 
      gaccaccag acatatcag ccgatggtc ctcttcaag 
      agaacaaaa caccacaaa ggctcgatc tattgtcta 
      gcttggtcg ccaatgggc gacctgatg gccaccggc 
      tcgaacgac aaaaccgtc aaactcatg cgattcaac 
      gccgacacc tcgaacctc gaaggcgaa gaaatcgaa 
      ttatcgatg cacgacggc acgatacgt gacttgtgt 
      ttcctggag gacacctcg aataaatca agccttctg 
      atcagcggt ggagccgga gattgcaaa atttacgta 
      accgactgc gcgacagga acgcccttc caggccctc 
      agcgggcac actgggcac attttgtcc ctatacaca 
      tggggcggt gcgatgttt gtcagcgga tcgcacgat 
      aagactgta cgattttgg gatttgcga acgaggggt 
      tgcgtcaat atggtgacg ccctgtacc atccctggg 
      tcacggcaa ggctcacct gtggcgagt ctttgcgtt 
      gacccgtcg gggaggctg ctggtgtcg gggcatgaa 
      gatagttcg tgcgttttg tacgatatt agaggcggg 
      aggagcgtc cagtgtttc aagccgcat tcggcagat 
      gtcaggtcg atcaggttt tcgccatcg gcctactat 
      ctactgtcg gcgggatat gacaataag ctggtgctt 
      actgatttg caaggttgg tattacatt tatttcaag 
      tcgttccgt gattccgct tctgaaatg taa
    • >TC032353 RB
      atgggcact gccatcaac catgcagct gccattaaa 
      aggctcgag gccctcttc aacgaggtt aagctcgag 
      caaaaacca gcaccagaa gtgcacgtc gaagtggaa 
      tcgaaaata agtacaaaa tgtgatgtg cacggaaaa 
      aacggacgg cgaggttcg ctaggaaat gttctgcat 
      attacaaat cggagagat tcgctgggg aatgttgtc 
      agtcaaaag agggactcc aaccagctt tatgccgga 
      cttttcgga aacggcgtt ggtccaagg agggaatca 
      atgcctgca cttaataat tacggcact tactatagg 
      cgccgtgac tctctggcc cttccgaac ggtccgccc 
      aaaaaagac atggagcat ccaagtcag tttgcttca 
      actttacgg aaggatcct ctgggatca tcaatggga 
      tcgttacgt aaagataac ggccgttgg ggccctcgt 
      agagactcc ctgggcgga tcaatgtcc tccctaaaa 
      tcaaacttc ttacaagtg cctaatcaa atgagacgt 
      aattcgaaa aattattcc accgattcg ctcgacggg 
      cgccgaaat tcctgggac cccaaccgc agaggaagc 
      tccggaagt tcatgttgg gaagaaccg atttggcaa 
      agcagaaag actattatt atggatgac caagaacaa 
      atttcacgc ggcgaacgc gaaaaaaaa ttagttgag 
      gtaccgccc gatgcggaa ctcatgtgt gaaattctt 
      cacaacgaa acgcaacct actgaagat acgacaccg 
      cctgaagaa gaagaaaaa cccacagag gaagaagag 
      aaaacagag gaggaagat gcggtacgt gtctgtgta 
      ttacaatta agtgaaatg ggcgattta atgctgaag 
      caaaagcag ccgcgtctg ccccattgg caaggcatc 
      gacctactg gcgaggaag ccgtggcct tccgcccaa 
      aggataaac acccagaag tcgcccttt aacttccgg 
      ccaccggaa cccccaacc tggagggaa cccaaccct 
      tactcctac caggcgccc ctttacaac tacccgaac 
      ccgaaacgg gaccattac ttcctctcg ccctattac 
      cgcaatgcc atgccgccg cctcctaac cccaatcgg 
      ctgctgatg atcgatccg agaagacgc ttcgttaag 
      cagaacgaa gattcgtgt tattgtcga tcgagatcg 
      atggaggac gttagggca gatgttgtt acaccggat 
      tttgagaac gatgtcaac ggcaatcgg gttccgatt 
      cccatgagg aaggatttc agcaaattt gcaaacagg 
      aggtctatg gataatctt ttggtggat acgaatttt 
      tcgccaccg aggaaacgc gccggaagg ctgcagacg 
      ataataatc aattggatt tttcaatta cagcctgtt 
      ccgaggaac tcatcagaa caagataat agcgacagt 
      aatggtgct agacctaga tttgtggct gttacaact 
      ttggaagat gttcaagct gtcagatgt gcagaattt 
      catcccggc ggtcaactg tatgctgtc ggttccaat 
      tcgaaaact ttacgaata tgtgcttat cccaaactc 
      agtgacctc agggaagac caccagaca tatcagccg 
      atggtcctc ttcaagaga acaaaacac cacaaaggc 
      tcgatctat tgtctagct tggtcgcca atgggcgac 
      ctgatggcc accggctcg aacgacaaa accgtcaaa 
      ctcatgcga ttcaacgcc gacacctcg aacctcgaa 
      ggcgaagaa atcgaatta tcgatgcac gacggcacg 
      atacgtgac ttgtgtttc ctggaggac acctcgaat 
      aaatcaagc cttctgatc agcggtgga gccggagat 
      tgcaaaatt tacgtaacc gactgcgcg acaggaacg 
      cccttccag gccctcagc gggcacact gggcacatt 
      ttgtcccta tacacatgg ggcggtgcg atgtttgtc 
      agcggatcg cacgataag actgtacga ttttgggat 
      ttgcgaacg aggggttgc gtcaatatg gtgacgccc 
      tgtaccatc cctgggtca cggcaaggc tcacctgtg 
      gcgagtctt tgcgttgac ccgtcgggg aggctgctg 
      gtgtcgggg catgaagat agttcgtgc gttttgtac 
      gatattaga ggcgggagg agcgtccag tgtttcaag 
      ccgcattcg gcagatgtc aggtcgatc aggttttcg 
      ccatcggcc tactatcta ctgtcggcg ggatatgac 
      aataagctg gtgcttact gatttgcaa ggttggtat 
      tacatttat ttcaagtcg ttccgtgat tccgcttct 
      gaaatgtaa
    mRNA sequence
    • >TC032353 RA
      tggaatgtg gccgcagtc atgggaacc tgctgacac 
      actcgcatc cttagtgtt gtgtgaaat tccaacgtg 
      aatgtttaa actttgtga atgaacagt aattcaaaa 
      tttgagccg aagtgttga acgaaagtg tttttggat 
      gttgttgga catttaagt gtttttggc gaaatgggc 
      actgccatc aaccatgca gctgccatt aaaaggctc 
      gaggccctc ttcaacgag gttaagctc gagcaaaaa 
      ccagcacca gaagtgcac gtcgaagtg gaatcgaaa 
      ataagtaca aaatgtgat gtgcacgga aaaaacgga 
      cggcgaggt tcgctagga aatgttctg catattaca 
      aatcggaga gattcgctg gggaatgtt gtcagtcaa 
      aagagggac tccaaccag ctttatgcc ggacttttc 
      ggaaacggc gttggtcca aggagggaa tcaatgcct 
      gcacttaat aattacggc acttactat aggcgccgt 
      gactctctg gcccttccg aacggtccg cccaaaaaa 
      gacatggag catccaagt cagtttgct tcaacttta 
      cggaaggat cctctggga tcatcaatg ggatcgtta 
      cgtaaagat aacggccgt tggggccct cgtagagac 
      tccctgggc ggatcaatg tcctcccta aaatcaaac 
      ttcttacaa gtgcctaat caaatgaga cgtaattcg 
      aaaaattat tccaccgat tcgctcgac gggcgccga 
      aattcctgg gaccccaac cgcagagga agctccgga 
      agttcatgt tgggaagaa ccgatttgg caaagcaga 
      aagactatt attatggat gaccaagaa caaatttca 
      cgcggcgaa cgcgaaaaa aaattagtt gaggtaccg 
      cccgatgcg gaactcatg tgtgaaatt cttcacaac 
      gaaacgcaa cctactgaa gatacgaca ccgcctgaa 
      gaagaagaa aaacccaca gaggaagaa gagaaaaca 
      gaggaggaa gatgcgcaa aagcagccg cgtctgccc 
      cattggcaa ggcatcgac ctactggcg aggaagccg 
      tggccttcc gcccaaagg ataaacacc cagaagtcg 
      ccctttaac ttccggcca ccggaaccc ccaacctgg 
      agggaaccc aacccttac tcctaccag gcgcccctt 
      tacaactac ccgaacccg aaacgggac cattacttc 
      ctctcgccc tattaccgc aatgccatg ccgccgcct 
      cctaacccc aatcggctg ctgatgatc gatccgaga 
      agacgcttc gttaagcag aacgaagat tcgtgttat 
      tgtcgatcg agatcgatg gaggacgtt agggcagat 
      gttgttaca ccggatttt gagaacgat gtcaacggc 
      aatcgggtt ccgattccc atgaggaag gatttcagc 
      aaatttgca aacaggagg tctatggat aatcttttg 
      gtggatacg aatttttcg ccaccgagg aaacgcgcc 
      ggaaggctg cagacgata ataatcaat tggattttt 
      caattacag cctgttccg aggaactca tcagaacaa 
      gataatagc gacagtaat ggtgctaga cctagattt 
      gtggctgtt acaactttg gaagatgtt caagctgtc 
      agatgtgca gaatttcat cccggcggt caactgtat 
      gctgtcggt tccaattcg aaaacttta cgaatatgt 
      gcttatccc aaactcagt gacctcagg gaagaccac 
      cagacatat cagccgatg gtcctcttc aagagaaca 
      aaacaccac aaaggctcg atctattgt ctagcttgg 
      tcgccaatg ggcgacctg atggccacc ggctcgaac 
      gacaaaacc gtcaaactc atgcgattc aacgccgac 
      acctcgaac ctcgaaggc gaagaaatc gaattatcg 
      atgcacgac ggcacgata cgtgacttg tgtttcctg 
      gaggacacc tcgaataaa tcaagcctt ctgatcagc 
      ggtggagcc ggagattgc aaaatttac gtaaccgac 
      tgcgcgaca ggaacgccc ttccaggcc ctcagcggg 
      cacactggg cacattttg tccctatac acatggggc 
      ggtgcgatg tttgtcagc ggatcgcac gataagact 
      gtacgattt tgggatttg cgaacgagg ggttgcgtc 
      aatatggtg acgccctgt accatccct gggtcacgg 
      caaggctca cctgtggcg agtctttgc gttgacccg 
      tcggggagg ctgctggtg tcggggcat gaagatagt 
      tcgtgcgtt ttgtacgat attagaggc gggaggagc 
      gtccagtgt ttcaagccg cattcggca gatgtcagg 
      tcgatcagg ttttcgcca tcggcctac tatctactg 
      tcggcggga tatgacaat aagctggtg cttactgat 
      ttgcaaggt tggtattac atttatttc aagtcgttc 
      cgtgattcc gcttctgaa atgtaaagg gtatttcaa 
      atttaaacg gtcacctaa aaattatac ttctgatta 
      tgtaaaaca aatctttat agcgcaaat aatatacca 
      ttttcaatt ac
    • >TC032353 RB
      tggaatgtg gccgcagtc atgggaacc tgctgacac 
      actcgcatc cttagtgtt gtgtgaaat tccaacgtg 
      aatgtttaa actttgtga atgaacagt aattcaaaa 
      tttgagccg aagtgttga acgaaagtg tttttggat 
      gttgttgga catttaagt gtttttggc gaaatgggc 
      actgccatc aaccatgca gctgccatt aaaaggctc 
      gaggccctc ttcaacgag gttaagctc gagcaaaaa 
      ccagcacca gaagtgcac gtcgaagtg gaatcgaaa 
      ataagtaca aaatgtgat gtgcacgga aaaaacgga 
      cggcgaggt tcgctagga aatgttctg catattaca 
      aatcggaga gattcgctg gggaatgtt gtcagtcaa 
      aagagggac tccaaccag ctttatgcc ggacttttc 
      ggaaacggc gttggtcca aggagggaa tcaatgcct 
      gcacttaat aattacggc acttactat aggcgccgt 
      gactctctg gcccttccg aacggtccg cccaaaaaa 
      gacatggag catccaagt cagtttgct tcaacttta 
      cggaaggat cctctggga tcatcaatg ggatcgtta 
      cgtaaagat aacggccgt tggggccct cgtagagac 
      tccctgggc ggatcaatg tcctcccta aaatcaaac 
      ttcttacaa gtgcctaat caaatgaga cgtaattcg 
      aaaaattat tccaccgat tcgctcgac gggcgccga 
      aattcctgg gaccccaac cgcagagga agctccgga 
      agttcatgt tgggaagaa ccgatttgg caaagcaga 
      aagactatt attatggat gaccaagaa caaatttca 
      cgcggcgaa cgcgaaaaa aaattagtt gaggtaccg 
      cccgatgcg gaactcatg tgtgaaatt cttcacaac 
      gaaacgcaa cctactgaa gatacgaca ccgcctgaa 
      gaagaagaa aaacccaca gaggaagaa gagaaaaca 
      gaggaggaa gatgcggta cgtgtctgt gtattacaa 
      ttaagtgaa atgggcgat ttaatgctg aagcaaaag 
      cagccgcgt ctgccccat tggcaaggc atcgaccta 
      ctggcgagg aagccgtgg ccttccgcc caaaggata 
      aacacccag aagtcgccc tttaacttc cggccaccg 
      gaaccccca acctggagg gaacccaac ccttactcc 
      taccaggcg cccctttac aactacccg aacccgaaa 
      cgggaccat tacttcctc tcgccctat taccgcaat 
      gccatgccg ccgcctcct aaccccaat cggctgctg 
      atgatcgat ccgagaaga cgcttcgtt aagcagaac 
      gaagattcg tgttattgt cgatcgaga tcgatggag 
      gacgttagg gcagatgtt gttacaccg gattttgag 
      aacgatgtc aacggcaat cgggttccg attcccatg 
      aggaaggat ttcagcaaa tttgcaaac aggaggtct 
      atggataat cttttggtg gatacgaat ttttcgcca 
      ccgaggaaa cgcgccgga aggctgcag acgataata 
      atcaattgg atttttcaa ttacagcct gttccgagg 
      aactcatca gaacaagat aatagcgac agtaatggt 
      gctagacct agatttgtg gctgttaca actttggaa 
      gatgttcaa gctgtcaga tgtgcagaa tttcatccc 
      ggcggtcaa ctgtatgct gtcggttcc aattcgaaa 
      actttacga atatgtgct tatcccaaa ctcagtgac 
      ctcagggaa gaccaccag acatatcag ccgatggtc 
      ctcttcaag agaacaaaa caccacaaa ggctcgatc 
      tattgtcta gcttggtcg ccaatgggc gacctgatg 
      gccaccggc tcgaacgac aaaaccgtc aaactcatg 
      cgattcaac gccgacacc tcgaacctc gaaggcgaa 
      gaaatcgaa ttatcgatg cacgacggc acgatacgt 
      gacttgtgt ttcctggag gacacctcg aataaatca 
      agccttctg atcagcggt ggagccgga gattgcaaa 
      atttacgta accgactgc gcgacagga acgcccttc 
      caggccctc agcgggcac actgggcac attttgtcc 
      ctatacaca tggggcggt gcgatgttt gtcagcgga 
      tcgcacgat aagactgta cgattttgg gatttgcga 
      acgaggggt tgcgtcaat atggtgacg ccctgtacc 
      atccctggg tcacggcaa ggctcacct gtggcgagt 
      ctttgcgtt gacccgtcg gggaggctg ctggtgtcg 
      gggcatgaa gatagttcg tgcgttttg tacgatatt 
      agaggcggg aggagcgtc cagtgtttc aagccgcat 
      tcggcagat gtcaggtcg atcaggttt tcgccatcg 
      gcctactat ctactgtcg gcgggatat gacaataag 
      ctggtgctt actgatttg caaggttgg tattacatt 
      tatttcaag tcgttccgt gattccgct tctgaaatg 
      taaagggta tttcaaatt taaacggtc acctaaaaa 
      ttatacttc tgattatgt aaaacaaat ctttatagc 
      gcaaataat ataccattt tcaattac
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY