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Gene information
General information
TC001580 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3084266..3095613
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001580
    iBeetle screen iB_03213

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 70.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 4 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 3 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: < 20
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001580 RA
      MVVVTHTEI CIPITYNRE FSRGRFQIR YVMPSARLT 
      LREEDVVRL ALEFLHNRE LHISQLSLE RETGVINGN 
      YSDDVLFLR QLILDGQWD DVLEFIQPL EALQAFNMR 
      KFSYTILKH KYIELLCIK SEANVQGNS VDNAVEEVV 
      KVLNDLEKF APSKEDYSN LCLLLTLPR LTDHLQYKD 
      WNPSNARVQ CFREVYPLV AEFLPGDRK STDANANNS 
      AKNDRLIQL IIKGILYES CVNYCQAKA TGTTESQSH 
      EMNFSRLLD GSVGFSDSD LSLLSWLQS VPSETFSVP 
      FEQRTLNVD VERLERPSL ETSWTEHML VTPIKPKTF 
      PHSAMPFTR PRSAADIMT RSLMPVLDG LPYGFAGGS 
      SAGQNGNPR LMALSTGDI NTNGPMSRS SFASFHLTG 
      IKNNKLMNT SVDRLFENE GEVFLSNSY AEFQQLPSI 
      QEQTSTPKL NTPTGNQAK SKSPERKVS NASTPEHKG 
      RESPASVGT ARSSRRDSL NEKPIAPVD SVPAGPVET 
      SLDRSYNGD LFREFQKQK QKFQETMAH KEREREELL 
      RQMNHENKL IDDWGLAKA DGRDPKGLQ EGLYDAVKV 
      GVHFYYNQQ
    CDS sequence
    • >TC001580 RA
      atggtggtt gtgactcac accgaaatt tgtatcccc 
      attacgtat aatagggag ttctccaga gggagattt 
      caaatacgg tacgtcatg ccgtcagct cggctcacc 
      ctccgtgag gaggacgtg gttcgactg gccctcgaa 
      ttcctgcac aaccgcgag ctgcacatt tcccagttg 
      agtctcgaa cgagaaacc ggcgtaatc aacggcaat 
      tactcggat gacgttctt ttcctgcga caactgata 
      ctcgatgga caatgggat gacgtactg gaattcatc 
      caaccgttg gaggctttg caggccttc aatatgcgc 
      aaatttagc tacacgatc ctcaagcac aagtacata 
      gagctgtta tgtatcaag tcggaggcc aacgtgcaa 
      gggaacagc gtcgacaac gcggtggaa gaagtcgtt 
      aaagtccta aacgatttg gaaaagttt gcgccttcc 
      aaggaggat tactcgaat ctgtgctta cttctgacg 
      ctgccgcgg ctcaccgat catttgcaa tacaaagac 
      tggaatccg agtaacgcc cgagtgcag tgctttcga 
      gaggtgtat cctctagta gctgaattt ctaccagga 
      gataggaaa tcgacagat gccaatgcc aataacagc 
      gcaaaaaac gacagactg atccaatta attatcaaa 
      ggcattctc tacgaatcc tgtgtgaat tactgtcaa 
      gccaaagcg acaggaacc accgaatct caatcgcac 
      gaaatgaat ttctcgaga ttacttgac ggcagtgtt 
      ggttttagt gactcagat cttagttta ttgtcttgg 
      ttgcaaagc gttccttcg gaaacgttc tctgtaccc 
      ttcgagcaa cgaaccctc aacgtggac gtggaacgt 
      ctggaaaga ccttccttg gagacttcc tggaccgag 
      cacatgctc gtcactcca attaagcca aagacgttc 
      ccgcactcg gcgatgcca ttcactcgt ccgcgttcg 
      gcggccgat atcatgacc cgttccctc atgccggtg 
      ttggacggg ttgccttac ggtttcgcc ggcggaagc 
      agcgccgga cagaacggc aacccacgt ctcatggct 
      ctcagcacc ggagacatc aacaccaac ggacccatg 
      tcccgaagt agtttcgcc agttttcac ctcaccgga 
      ataaaaaac aacaaactg atgaacaca tcagtggac 
      cgcctcttc gaaaacgag ggcgaagtg ttcctgagt 
      aacagttac gcagaattc caacaactt ccctcgatt 
      caggaacag acaagtacg ccgaaatta aacacaccc 
      actggaaat caagcaaag tcgaaaagt ccggagagg 
      aaggtgtcg aacgcctcg actccggaa cataaaggg 
      agggagtcg ccggcatcg gttggaaca gcgaggtct 
      tcaaggagg gattctttg aatgagaaa ccgatcgct 
      ccagtggat agtgtacca gctgggcca gttgagacg 
      agtttagat aggagttac aatggggat ctttttaga 
      gaatttcag aagcagaaa cagaagttt caggaaacg 
      atggcgcat aaggaacgg gaaagggaa gagttgttg 
      cggcagatg aatcatgag aataaattg atcgatgat 
      tggggactt gctaaagct gatggcagg gaccctaag 
      ggactacag gaaggactc tacgatgcc gttaaggta 
      ggagtgcat ttttattac aaccaacag taa
    mRNA sequence
    • >TC001580 RA
      cgtctgaga aaactgcta caaaaagtt taaattttt 
      gtacgtgtc tctttgagt ggcggtgac ttgtgtgca 
      aaaatatac aaaatgaag attcaaaat cgtggtgaa 
      gcatggtgg ttgtgactc acaccgaaa tttgtatcc 
      ccattacgt ataataggg agttctcca gagggagat 
      ttcaaatac ggtacgtca tgccgtcag ctcggctca 
      ccctccgtg aggaggacg tggttcgac tggccctcg 
      aattcctgc acaaccgcg agctgcaca tttcccagt 
      tgagtctcg aacgagaaa ccggcgtaa tcaacggca 
      attactcgg atgacgttc ttttcctgc gacaactga 
      tactcgatg gacaatggg atgacgtac tggaattca 
      tccaaccgt tggaggctt tgcaggcct tcaatatgc 
      gcaaattta gctacacga tcctcaagc acaagtaca 
      tagagctgt tatgtatca agtcggagg ccaacgtgc 
      aagggaaca gcgtcgaca acgcggtgg aagaagtcg 
      ttaaagtcc taaacgatt tggaaaagt ttgcgcctt 
      ccaaggagg attactcga atctgtgct tacttctga 
      cgctgccgc ggctcaccg atcatttgc aatacaaag 
      actggaatc cgagtaacg cccgagtgc agtgctttc 
      gagaggtgt atcctctag tagctgaat ttctaccag 
      gagatagga aatcgacag atgccaatg ccaataaca 
      gcgcaaaaa acgacagac tgatccaat taattatca 
      aaggcattc tctacgaat cctgtgtga attactgtc 
      aagccaaag cgacaggaa ccaccgaat ctcaatcgc 
      acgaaatga atttctcga gattacttg acggcagtg 
      ttggtttta gtgactcag atcttagtt tattgtctt 
      ggttgcaaa gcgttcctt cggaaacgt tctctgtac 
      ccttcgagc aacgaaccc tcaacgtgg acgtggaac 
      gtctggaaa gaccttcct tggagactt cctggaccg 
      agcacatgc tcgtcactc caattaagc caaagacgt 
      tcccgcact cggcgatgc cattcactc gtccgcgtt 
      cggcggccg atatcatga cccgttccc tcatgccgg 
      tgttggacg ggttgcctt acggtttcg ccggcggaa 
      gcagcgccg gacagaacg gcaacccac gtctcatgg 
      ctctcagca ccggagaca tcaacacca acggaccca 
      tgtcccgaa gtagtttcg ccagttttc acctcaccg 
      gaataaaaa acaacaaac tgatgaaca catcagtgg 
      accgcctct tcgaaaacg agggcgaag tgttcctga 
      gtaacagtt acgcagaat tccaacaac ttccctcga 
      ttcaggaac agacaagta cgccgaaat taaacacac 
      ccactggaa atcaagcaa agtcgaaaa gtccggaga 
      ggaaggtgt cgaacgcct cgactccgg aacataaag 
      ggagggagt cgccggcat cggttggaa cagcgaggt 
      cttcaagga gggattctt tgaatgaga aaccgatcg 
      ctccagtgg atagtgtac cagctgggc cagttgaga 
      cgagtttag ataggagtt acaatgggg atcttttta 
      gagaatttc agaagcaga aacagaagt ttcaggaaa 
      cgatggcgc ataaggaac gggaaaggg aagagttgt 
      tgcggcaga tgaatcatg agaataaat tgatcgatg 
      attggggac ttgctaaag ctgatggca gggacccta 
      agggactac aggaaggac tctacgatg ccgttaagg 
      taggagtgc atttttatt acaaccaac agtaataat 
      aaaattgag caaactgt
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY