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Gene information
General information
TC001577 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3420168..3426085
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001577
    iBeetle screen iB_03212

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 40.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 1 (9 dpi)
    Days post injection


    Analysis of larval stage 1 cuticle
    number of eggs/embryos/larvae on slide: > 50
    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001577 RA
      MKLSVQETK DLHKYTKFF ALKSAQVII QSRVAERIS 
      TQCKPNTQG TDWFNLHIN DHPDVIAET KKALNGEII 
      SPCFPLCIE ISLRTSEGD QMTLESWCL SLLPEQCDP 
      TLKNIPSVY NRMGLLLKS LLSVTRVTP AYKLSRMQG 
      ADSYVMCYR IYLGQPVLH SLGEDYKQT RIGQICTPI 
      GTLQFSVAY RTKMTISPT QTGKNSIMV KSDHFNSNS 
      NTRRHRNGD DINDLTKPM RPGPFADPK PKKTVEPQL 
      PGVTRLLAM GKNKPRPTK NSENAENEV IENPRLIEE 
      DESSKKEIS NSPTKSIPK MTSSNSSRL SIFSNLKSV 
      DEEYWMQEL SAPFANRGP MGELMQFYK QCKKSPPLQ 
      SPQKNPTPE EITEMIKMY ESQLPEYDA LVQSFCESP 
      DNN
    CDS sequence
    • >TC001577 RA
      atgaagctc agcgtccag gaaacgaag gatctgcac 
      aagtacacc aagtttttc gccctgaag tcggcccag 
      gtgatcatc cagtcgcgg gtcgccgag aggatctcc 
      acccagtgt aagccgaac acccagggt acggattgg 
      ttcaacctg cacatcaac gaccaccct gatgtcatc 
      gccgagacg aagaaagcc ctcaatgga gagataatc 
      tcgccgtgt tttccgctc tgcatcgaa atctcgctg 
      cggacgtct gaaggggat caaatgacg ttggaatcc 
      tggtgcctt agtctgcta cctgaacaa tgtgacccg 
      acccttaaa aacatcccc tctgtctat aacagaatg 
      gggcttctc ttgaaatcg ttactatca gttactcga 
      gtgacccca gcttataaa ctttcacgc atgcaaggt 
      gcggattcg tacgttatg tgttatcgt atttacttg 
      ggtcagcct gtactacac agccttggc gaagattat 
      aaacaaaca aggataggg caaatttgt acgcccatc 
      gggacgttg cagttttct gtagcttac agaacgaaa 
      atgaccatt tcacccact cagactggg aaaaactca 
      atcatggtc aagagtgac cacttcaac agtaattcc 
      aacaccagg aggcacagg aatggtgat gatatcaat 
      gacttgaca aaaccaatg cgacctggt ccgtttgcg 
      gatcctaag ccgaaaaaa acggtagaa cctcagctc 
      cccggtgtc acacgatta ctagcgatg gggaaaaat 
      aaaccacga cccaccaaa aattcagaa aatgctgaa 
      aacgaagtg atagaaaac ccaagactt atagaagaa 
      gatgaatct agtaaaaaa gaaatttca aattcaccc 
      acgaaatca ataccaaaa atgacatca tcaaattca 
      tctagactt tcaattttt agtaattta aaatcggtg 
      gatgaggag tactggatg caggagctg agcgcaccg 
      tttgcaaac agaggcccc atgggcgag ctgatgcag 
      ttttacaaa cagtgcaaa aagtcgccc cctttgcaa 
      tcccctcag aaaaaccca acacctgaa gaaatcacc 
      gaaatgatc aaaatgtac gaatcgcag ttgcccgag 
      tacgatgct ctagtgcag tcattttgt gaatcaccc 
      gataataac taa
    mRNA sequence
    • >TC001577 RA
      actttttaa attctcgta gtttttgct ttgtaactg 
      cattttggt gttgtgctc gtcaaggtt aggcgtcta 
      aaactgccc cagacgagc ccttttcgc agacgcccc 
      ctttgcgcg catttggag cgcggcctt ccaacgccc 
      cacactcgt ggtattctt ggatacgtg cacgttcgt 
      tatcacacg tagttccag aagagtggg tgtcccagg 
      gctgcgccc gccagtgca atgtgcgtt tagtgactg 
      gcttcacgc tttctcatg tgtgggaaa atgtgctag 
      tgttcgccg ccacgatga agctcagcg tccaggaaa 
      cgaaggatc tgcacaagt acaccaagt ttttcgccc 
      tgaagtcgg cccaggtga tcatccagt cgcgggtcg 
      ccgagagga tctccaccc agtgtaagc cgaacaccc 
      agggtacgg attggttca acctgcaca tcaacgacc 
      accctgatg tcatcgccg agacgaaga aagccctca 
      atggagaga taatctcgc cgtgttttc cgctctgca 
      tcgaaatct cgctgcgga cgtctgaag gggatcaaa 
      tgacgttgg aatcctggt gccttagtc tgctacctg 
      aacaatgtg acccgaccc ttaaaaaca tcccctctg 
      tctataaca gaatggggc ttctcttga aatcgttac 
      tatcagtta ctcgagtga ccccagctt ataaacttt 
      cacgcatgc aaggtgcgg attcgtacg ttatgtgtt 
      atcgtattt acttgggtc agcctgtac tacacagcc 
      ttggcgaag attataaac aaacaagga tagggcaaa 
      tttgtacgc ccatcggga cgttgcagt tttctgtag 
      cttacagaa cgaaaatga ccatttcac ccactcaga 
      ctgggaaaa actcaatca tggtcaaga gtgaccact 
      tcaacagta attccaaca ccaggaggc acaggaatg 
      gtgatgata tcaatgact tgacaaaac caatgcgac 
      ctggtccgt ttgcggatc ctaagccga aaaaaacgg 
      tagaacctc agctccccg gtgtcacac gattactag 
      cgatgggga aaaataaac cacgaccca ccaaaaatt 
      cagaaaatg ctgaaaacg aagtgatag aaaacccaa 
      gacttatag aagaagatg aatctagta aaaaagaaa 
      tttcaaatt cacccacga aatcaatac caaaaatga 
      catcatcaa attcatcta gactttcaa tttttagta 
      atttaaaat cggtggatg aggagtact ggatgcagg 
      agctgagcg caccgtttg caaacagag gccccatgg 
      gcgagctga tgcagtttt acaaacagt gcaaaaagt 
      cgccccctt tgcaatccc ctcagaaaa acccaacac 
      ctgaagaaa tcaccgaaa tgatcaaaa tgtacgaat 
      cgcagttgc ccgagtacg atgctctag tgcagtcat 
      tttgtgaat cacccgata ataactaaa gtaagagtt 
      cgttttgta attaatttt tgtgtttat ttattgtca 
      cgtcataaa attagcaaa tgtcacatg taaattgtt 
      tccttctgc aaaagactg ataagaata taagagcga 
      gtgtaaaaa cgttgtaca tactgaaca tgaaattac 
      ttaaatgag acaacaaag ctgttgaac ataagttta 
      ttttttaaa gttgtaaat aatacagtt catatattt 
      acaagtttt tgaacattt gaaatttta agcggttgt 
      atttagcct attatcctg tgcattgat ctgctaaag 
      aatcgagct caaaagcat ctaattttt tgttggaag 
      ttaatgaga cagagcaag gccgccgaa gcgaggcct 
      tgacctgat ctgtgcaac cagaaaaga aagtaaatt 
      atgtggttt ttataaatt gttgcgttc cgtactaat 
      ttaattaag ttattagat actttcgag cattttttc 
      acaaaaaat agaactcac ttgtgtagt actttggta 
      ttacttcaa aacatgttt tataaatag tacatactt 
      tattttacg ttctatttg ctcaatatt taatttgtt 
      gttgcgtat tatttatta taagtatct acaatatat 
      ttttttgtg ttttggtcc attttttgt ataaaatta 
      ttttaactg gtacaaaag atttatcta tcaaatcaa 
      tacaacatc aaatacatt atttattat tataacttt 
      gtgttttat ttaaaatcg ttccaatac tgaattttc 
      tgattgtcg tcgaaagaa attcaacag taagtattc 
      caacgaaaa aggttggtt gcacaagca actatcgac 
      gaaatattg aaggtaata aattacaat aattaaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY