Status
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Gene information
General information
TC001573 Gene

The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser.

NC_007418.3: 3473510..3478486
Sequence information
Transcripts / Proteins (1) Get mRNA sequence
Get CDS sequence
Get protein sequence
Ortholog information (from OrthoDB)

The information about the homolog genes in Drosophila is provided by OrthoDB http://www.orthodb.org/. The homologs are sorted according to their similarity score. For an orthology statement additional analysis are required.
The data basis for the orthologs is OrthoDB v9 containing OGS3 for Tribolium castaneum and 2015_04 (r6.07) for Drosophila melanogaster.

Closest fly homologs
    TC001573
    Gene ontology

    GO terms for Tribolium

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    There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.


    GO terms for the homologous Drosophila genes


    Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.


    Gene
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    The Tribolium gene the GO term is assigned to.
    GO ID *
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    The ID of the GO term you assign to the gene. Please enter only the ID of the GO term with proceeding 'GO:'. This information is mandatory.
    Evidence
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    The evidence code describes with which method the function or location, expressed by the GO term, was show for this gene. Please refer to this page for a definition of the evidence code and a decision tree.
    Reference *
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    The reference where the function or location is described for the gene. Please enter a PubMed ID. This information is mandatory.
    Quotation
    If you paste here the relevant section of the reference it is for us a great help to review your annotation. The quotation may be also be displayed to the users of iBeetle-Base.
    Name
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    Email
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    * : mandatory fields
    For help on how to submit larger datasets or non experimental data please contact us.
    iBeetle screen iB_03210

    Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.

    iB sequence
    Phenotype after pupal injection
    Usually 10 injected animals

    Pupal injection procedure

    Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.

    3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.

    9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.

    11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.

    Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.

    13 dpi: Egg productivity and Ovary analysis
    The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi). In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.

    14 dpi: Analysis of embryonic musculature and early embryonic development
    Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.

    15 dpi: Analysis of larval instar 1 cuticle
    Offspring of injected females were analysed and cuticle phenotypes were annotated.

    22 dpi: Stink gland analysis
    Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.

    Terms used in the pupal injection procedure

    larval pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    empty eggs
    Just empty shells are visible, no cuticle has been developed.
    strong defects
    L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
    strongly fragmented (cuticle crumbs)
    No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
    cuticle remnants (with unidentifiable segments)
    Segments or parts of appendages are recognizable, but identity is not clear.
    cuticle remnants (with some identifiable segments)
    At least one segment or appendage is clearly regonizable.
    number of eggs on slide
    affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
    For the embryonic muscle analysis the hatched as well as the not hatched were analysed.
    total number of affected eggs/embryos/larvae
    Summary of all different phenotypes/ phenotypic series which were annotated.
    inside-out
    The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    Please see the help page for more information
    Metamorphosis and survival

    Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)

    eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)
    Days post injection


    adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
    Days post injection


    Phenotype after larval injection
    Usually 10 injected animals

    Larval injection procedure

    Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.

    11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.

    16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.

    19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.

    22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.

    41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).

    Terms used in the larval injection procedure

    pupal/ adult pantagmatic defects
    At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
    pupal molt not fulfilled
    The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
    eclosion not fulfilled
    The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
    starved
    Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
    Metamorphosis and survival

    Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
    Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)

    RNAi sequence
    Primers
    Left: Right:
    Protein sequence
    • >TC001573 RA
      MGKRKNQAL IDSDSSNDS TSDLDSEFL NLAKKKNRK 
      KKSPRSNSD SEYEGKSDS DEGRNNKKP KRDESSDSE 
      FEKKEEAKN NSEPEEGEV SDSSISDEE YNDGYDDQL 
      MGDEEDRAR LASLTEKER ETEIFKRIE QRELMKTRF 
      EIEKKLRQA KKAERAREK PHKHKDKEK SRDHSKLQT 
      DFSSIDHKE RSKERKKNI EENRGKVDK RVNAMAELK 
      ARREDKQKR EEAEEKRKE EQRKKEEED ALHTNKSNV 
      KLKASDIYS DDSESEGGK SSPQPPRQS VGSVRSRSS 
      SSSSSSDNE QEEEEKPAY VPTKLDMNN IRLSRHKLE 
      RFVHMPFFD RIVKGCYVK IGIGQHNNM PVYRVAEVT 
      NVYETAKIY NLGNTRTNK GLRVRHGNQ ERVFRLEFV 
      SNQEFTETE YHKWIEAST AASNPLPTK EHIEQKQAD 
      IKEALNYEF NEQDIERII REKERFKPN PHNYAMRKT 
      QLMKERDSA LARGAEDLA RELTQRISD LEERASELD 
      KMRTSTISS ISYINDRNR KRNVEEAEK AIMAEVKAN 
      KGKKIDDPF TRRSTKPRM NFKAAPEEK AEVVVEKKP 
      EKVAVEVEN PKVAAQPAQ VTQDDLFSA HDFDIKIDL 
      DVPLPVASV NVTPKTVTK DLAPRRSLN LQDYKKKRG 
      LI
    CDS sequence
    • >TC001573 RA
      atgggaaaa cgcaaaaat caagctttg attgacagt 
      gattcgagc aatgattca actagcgat ttggactct 
      gaatttttg aatttggcg aagaaaaag aacaggaag 
      aagaaatcg ccaaggtct aactcggat tcggaatac 
      gagggcaaa tctgacagt gatgagggt cgaaataac 
      aagaaaccc aaacgagat gagtcctct gactccgaa 
      tttgaaaag aaagaggag gcgaaaaat aactcagag 
      cctgaggaa ggtgaagtc tcagactct tcaatatct 
      gatgaagaa tataacgac gggtatgac gaccaactt 
      atgggcgat gaagaagac agagcccgc ttagccagt 
      ttaactgag aaagaacga gaaacggaa attttcaaa 
      agaatcgag caacgagaa ttgatgaaa acgcgattt 
      gagattgag aaaaagttg agacaggcg aagaaagcg 
      gaaagggca cgggagaaa cctcacaag cacaaagac 
      aaagagaag agcagggac cacagtaaa ctccaaacc 
      gatttcagt tcaatagac cacaaggaa cgtagcaaa 
      gagcggaag aaaaacatt gaggaaaat cgcggcaaa 
      gttgacaaa cgtgtcaac gccatggcc gaactcaaa 
      gccagaaga gaagacaag caaaagcgg gaagaagcc 
      gaagaaaaa cgcaaggaa gaacagagg aagaaggaa 
      gaggaagac gctctgcac acaaacaag agcaacgta 
      aagttaaag gcgtcagat atttattca gatgacagc 
      gaaagtgaa ggaggtaaa agttcacct cagccacca 
      agacaatct gtggggtct gtcagatcc cgctcttcg 
      tctagttca agttcgagt gataatgaa caagaagaa 
      gaggaaaaa cccgcttat gtacctacg aaacttgac 
      atgaataat attcggttg tcgagacat aaattagaa 
      agatttgta catatgccg ttctttgat cgtattgtt 
      aaaggatgt tacgtaaaa atcggtatt gggcaacat 
      aataatatg cctgtttat cgggtggcc gaagtcaca 
      aatgtttat gaaactgcc aagatttac aatttggga 
      aacaccaga acaaacaag ggattgcgt gtacgacac 
      ggaaatcag gagcgagtt ttccgccta gaatttgta 
      tccaatcaa gaattcacc gagactgag tatcacaag 
      tggatagag gccagcaca gctgctagt aatccttta 
      cccacaaaa gaacacatt gaacagaaa caagccgac 
      ataaaagaa gccttaaat tacgaattt aacgaacag 
      gatattgaa cgaattata cgcgaaaaa gagcggttc 
      aagccaaac ccccacaat tatgcaatg cgcaaaacc 
      caactaatg aaagaaaga gactcagcc ctggctcgc 
      ggggctgaa gatttggcg agagaatta acccaaagg 
      atctctgat ttggaagaa agggcgtca gagttggac 
      aaaatgaga acatcgacc atttcaagt atttcatac 
      atcaacgac cggaacagg aaacgcaac gttgaagag 
      gccgaaaag gcgattatg gccgaagtt aaagcaaac 
      aaaggcaaa aagatcgat gacccgttc acaagacgc 
      tcgacaaaa ccaagaatg aatttcaag gcagctcct 
      gaagagaaa gccgaagta gtggttgag aaaaagcca 
      gagaaggtt gcagtggaa gtggaaaac ccgaaagta 
      gcagcgcag ccggctcaa gttactcag gacgatctc 
      ttctcagct catgacttt gatattaaa attgactta 
      gatgttcct ttaccagtg gctagcgta aacgttaca 
      cccaaaaca gtaacaaag gaccttgct ccgagacga 
      agcttgaac ttgcaagac tacaagaag aagagagga 
      cttatatag
    mRNA sequence
    • >TC001573 RA
      cttgaaaaa ggaacagaa agaaatgaa aaatagtaa 
      tggtggtgg cagttagtg gttgtgttg ttggatttt 
      gatttaatt taaatttct gaagcaaaa ttgatcatc 
      cagtttgaa atcccaaga tgggaaaac gcaaaaatc 
      aagctttga ttgacagtg attcgagca atgattcaa 
      ctagcgatt tggactctg aatttttga atttggcga 
      agaaaaaga acaggaaga agaaatcgc caaggtcta 
      actcggatt cggaatacg agggcaaat ctgacagtg 
      atgagggtc gaaataaca agaaaccca aacgagatg 
      agtcctctg actccgaat ttgaaaaga aagaggagg 
      cgaaaaata actcagagc ctgaggaag gtgaagtct 
      cagactctt caatatctg atgaagaat ataacgacg 
      ggtatgacg accaactta tgggcgatg aagaagaca 
      gagcccgct tagccagtt taactgaga aagaacgag 
      aaacggaaa ttttcaaaa gaatcgagc aacgagaat 
      tgatgaaaa cgcgatttg agattgaga aaaagttga 
      gacaggcga agaaagcgg aaagggcac gggagaaac 
      ctcacaagc acaaagaca aagagaaga gcagggacc 
      acagtaaac tccaaaccg atttcagtt caatagacc 
      acaaggaac gtagcaaag agcggaaga aaaacattg 
      aggaaaatc gcggcaaag ttgacaaac gtgtcaacg 
      ccatggccg aactcaaag ccagaagag aagacaagc 
      aaaagcggg aagaagccg aagaaaaac gcaaggaag 
      aacagagga agaaggaag aggaagacg ctctgcaca 
      caaacaaga gcaacgtaa agttaaagg cgtcagata 
      tttattcag atgacagcg aaagtgaag gaggtaaaa 
      gttcacctc agccaccaa gacaatctg tggggtctg 
      tcagatccc gctcttcgt ctagttcaa gttcgagtg 
      ataatgaac aagaagaag aggaaaaac ccgcttatg 
      tacctacga aacttgaca tgaataata ttcggttgt 
      cgagacata aattagaaa gatttgtac atatgccgt 
      tctttgatc gtattgtta aaggatgtt acgtaaaaa 
      tcggtattg ggcaacata ataatatgc ctgtttatc 
      gggtggccg aagtcacaa atgtttatg aaactgcca 
      agatttaca atttgggaa acaccagaa caaacaagg 
      gattgcgtg tacgacacg gaaatcagg agcgagttt 
      tccgcctag aatttgtat ccaatcaag aattcaccg 
      agactgagt atcacaagt ggatagagg ccagcacag 
      ctgctagta atcctttac ccacaaaag aacacattg 
      aacagaaac aagccgaca taaaagaag ccttaaatt 
      acgaattta acgaacagg atattgaac gaattatac 
      gcgaaaaag agcggttca agccaaacc cccacaatt 
      atgcaatgc gcaaaaccc aactaatga aagaaagag 
      actcagccc tggctcgcg gggctgaag atttggcga 
      gagaattaa cccaaagga tctctgatt tggaagaaa 
      gggcgtcag agttggaca aaatgagaa catcgacca 
      tttcaagta tttcataca tcaacgacc ggaacagga 
      aacgcaacg ttgaagagg ccgaaaagg cgattatgg 
      ccgaagtta aagcaaaca aaggcaaaa agatcgatg 
      acccgttca caagacgct cgacaaaac caagaatga 
      atttcaagg cagctcctg aagagaaag ccgaagtag 
      tggttgaga aaaagccag agaaggttg cagtggaag 
      tggaaaacc cgaaagtag cagcgcagc cggctcaag 
      ttactcagg acgatctct tctcagctc atgactttg 
      atattaaaa ttgacttag atgttcctt taccagtgg 
      ctagcgtaa acgttacac ccaaaacag taacaaagg 
      accttgctc cgagacgaa gcttgaact tgcaagact 
      acaagaaga agagaggac ttatatagt atttattaa 
      tttattata tttttaaca aaataaatt ttagacctt 
      aaatgttac acttaaaca ttttttatt gttaattaa 
      gtttcattt tatttggaa acgcgttat aattttaca 
      gtgattttt aaggaatga attaattct gtacttttg 
      tagcctgtt gttagatct gtttgtgat tacgaattg 
      tttcacaaa tttgaaata atagacaaa caaggaa
    The data is licensed under a Creative Commons Attribution 3.0 Unported License. (CC-BY) CC-BY