| General information | |||
| TC001573 | Gene
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
NC_007418.3: 3473510..3478486 | |
| Sequence information | |||
| Transcripts / Proteins (1) | Get mRNA sequence
Get CDS sequence Get protein sequence |
||
| Ortholog information (from OrthoDB)
The information about the homolog genes in Drosophila
is provided by OrthoDB http://www.orthodb.org/.
The homologs are sorted according to their similarity score. For an orthology statement additional
analysis are required. | |||
| Closest fly homologs |
|
TC001573 | |
GO terms for Tribolium
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There are no GO terms for Tribolium at the moment. You can help the community by providing some GO terms for this gene.
GO terms for the homologous Drosophila genes
Assign a GO term to this Tribolium gene by filling in the fields. Repeat to add several GO terms. Search AmiGO for the correct GO ID. Use only the most specific term - use "graph views" to browse related terms ("child terms" are more specific; the more general "parent" terms will be automatically linked). Only information based on Tribolium data should be entered - do not define terms just based on Drosophila knowledge. We will review this information and submit the annotation to the Gene Ontology consortium.
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* : mandatory fields
Attention! iBeetle is a first pass screen. Hence, the documented phenotypes need to be confirmed by independent experiments using non-overlapping dsRNA fragments! Phenotypes with a penetrance > 50% are frequently reproduced. See Schmitt-Engel et al. 2015 for details on reproducibility.
| General information | ||||
| iB_03210 | iB fragment
The sequence from the iB fragment is matched against the genes from the official gene set. The matche(s) is/are visualizied in a genome browser. |
Get FastA Get primers |
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Pupal injection procedure
Day 0: 10 female pupae of the pBA19 strain (muscle enhancer trap line) were injected with dsRNA.
3 days post injection (dpi): Hatch control: Pupal and adult lethality as well as metamorphosis defects (molting, eclosion) were documented. For mating, 4 males of the black strain were added.
9 dpi: First egg-lay was collected and incubated for cuticle analysis. Adult lethality and egg production (reduced/ no egg-lay) was documented.
11 dpi: Second egg-lay was collected and incubated for embryonic muscle analysis. Adult lethality and egg production (reduced/ no egg-lay) were documented.
Note: The adult morphology was not analyzed systematically at 3/9/11 dpi. Only obvious phenotypes, visible without magnification, have been annotated.
13 dpi: Egg productivity and Ovary analysis
The percentage of hatched larvae was documented and not hatched larvae/ eggs were embedded for cuticle analysis (15 dpi).
In case of a reduction of egg production, 4 injected females were dissected to analyze the gross morphology of the ovaries.
14 dpi: Analysis of embryonic musculature and early embryonic development
Offspring of the injected females (hatched and not hatched larvae/ eggs) were analysed for embryonic lethality and muscle defects.
15 dpi: Analysis of larval instar 1 cuticle
Offspring of injected females were analysed and cuticle phenotypes were annotated.
22 dpi: Stink gland analysis
Documentation of defects in abdominal and thoracic stink glands (colour, size, content) of the injected femals.
Terms used in the pupal injection procedure
- larval pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- empty eggs
- Just empty shells are visible, no cuticle has been developed.
- strong defects
- L1 cuticle larvae which show severe defects. These strong defects are subdivided into three categories
- strongly fragmented (cuticle crumbs)
- No segments or appendages are identifiable, only residues of cuticle and bristles can be identified.
- cuticle remnants (with unidentifiable segments)
- Segments or parts of appendages are recognizable, but identity is not clear.
- cuticle remnants (with some identifiable segments)
- At least one segment or appendage is clearly regonizable.
- number of eggs on slide
- affected embryos usually (not always) do not hatch. Only the not hatched were used for cuticle analysis.
For the embryonic muscle analysis the hatched as well as the not hatched were analysed. - total number of affected eggs/embryos/larvae
- Summary of all different phenotypes/ phenotypic series which were annotated.
- inside-out
- The L1 larva shows a partial or complete inversion., e.g. bristles, appendages or parts of the abdominal segments are inverted into the interior of the cuticle.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
Lethality 11 days after pupal injection: 80.0% (includes death as pupa, adult)
eclosion not fulfilled (with pupal and adult features) - Number of animals showing the phenotype: 3 (3 dpi)adult lethality - Number of animals showing the phenotype: 5 (9 dpi)
Larval injection procedure
Day 0: 10 female L5-6 larvae of the D17Xhom strain (females express RFP in the eyes and in the CNS; pupae express GFP in the thorax) were injected with dsRNA.
11 days post injection (dpi): Pupal morphology Documentation of larval/ pupal death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of pupal head and appendages, pupal thorax, pupal thoracic musculature, pupal legs, pupal wings, pupal abdomen and pupal genital lobes.
16 dpi: Adult morphology Documentation of larval/ pupal and adult death and defects of metamorphosis (eclosion, delayed development). Screening for alterations of adult head and appendages, adult thorax, adult elytra and hindwings, adult legs and cuticle properties. For mating 4 males of the black strain were added.
19 dpi: Sieving and egg-lay Documentation of adult lethality and enduring metamorphosis defects.
22 dpi: Ovary analysis (Fertility) In case of a reduction of egg production 4 females were dissected and the gross morphology of the ovaries were analysed.
41 dpi: Fertility and stink gland analysis Documentation of defects in abdominal and thoracic stink glands development (colour, size, content) of the injected females. Check for fertility of injected larvae (no or decreased offspring).
Terms used in the larval injection procedure
- pupal/ adult pantagmatic defects
- At least two tagmata (head, thorax, abdomen or terminus) show similar/ comparable defects.
- pupal molt not fulfilled
- The hatching from the larva to the pupa is interrupted. Either it dies as prepupa or the phenotype shows larval as well as pupal features.
- eclosion not fulfilled
- The emergence of the adult from the pupa stage is interrupted. This phenotype shows pupal as well as adult features.
- starved
- Dissected females show a strong resorption of fatbody predominately in the abdomen and the oogenesis is blocked. These are considered as eventually lethal phenotypes and the blocked oogenesis probably a secondary defect due to starvation.
Lethalities 11 days after larval injection: % (includes death as larva, prepupa, pupa)
Lethalities 22 days after larval injection: % (includes death as larva, prepupa, pupa, adult)
- >TC001573 RA
MGKRKNQAL IDSDSSNDS TSDLDSEFL NLAKKKNRK KKSPRSNSD SEYEGKSDS DEGRNNKKP KRDESSDSE FEKKEEAKN NSEPEEGEV SDSSISDEE YNDGYDDQL MGDEEDRAR LASLTEKER ETEIFKRIE QRELMKTRF EIEKKLRQA KKAERAREK PHKHKDKEK SRDHSKLQT DFSSIDHKE RSKERKKNI EENRGKVDK RVNAMAELK ARREDKQKR EEAEEKRKE EQRKKEEED ALHTNKSNV KLKASDIYS DDSESEGGK SSPQPPRQS VGSVRSRSS SSSSSSDNE QEEEEKPAY VPTKLDMNN IRLSRHKLE RFVHMPFFD RIVKGCYVK IGIGQHNNM PVYRVAEVT NVYETAKIY NLGNTRTNK GLRVRHGNQ ERVFRLEFV SNQEFTETE YHKWIEAST AASNPLPTK EHIEQKQAD IKEALNYEF NEQDIERII REKERFKPN PHNYAMRKT QLMKERDSA LARGAEDLA RELTQRISD LEERASELD KMRTSTISS ISYINDRNR KRNVEEAEK AIMAEVKAN KGKKIDDPF TRRSTKPRM NFKAAPEEK AEVVVEKKP EKVAVEVEN PKVAAQPAQ VTQDDLFSA HDFDIKIDL DVPLPVASV NVTPKTVTK DLAPRRSLN LQDYKKKRG LI
- >TC001573 RA
atgggaaaa cgcaaaaat caagctttg attgacagt gattcgagc aatgattca actagcgat ttggactct gaatttttg aatttggcg aagaaaaag aacaggaag aagaaatcg ccaaggtct aactcggat tcggaatac gagggcaaa tctgacagt gatgagggt cgaaataac aagaaaccc aaacgagat gagtcctct gactccgaa tttgaaaag aaagaggag gcgaaaaat aactcagag cctgaggaa ggtgaagtc tcagactct tcaatatct gatgaagaa tataacgac gggtatgac gaccaactt atgggcgat gaagaagac agagcccgc ttagccagt ttaactgag aaagaacga gaaacggaa attttcaaa agaatcgag caacgagaa ttgatgaaa acgcgattt gagattgag aaaaagttg agacaggcg aagaaagcg gaaagggca cgggagaaa cctcacaag cacaaagac aaagagaag agcagggac cacagtaaa ctccaaacc gatttcagt tcaatagac cacaaggaa cgtagcaaa gagcggaag aaaaacatt gaggaaaat cgcggcaaa gttgacaaa cgtgtcaac gccatggcc gaactcaaa gccagaaga gaagacaag caaaagcgg gaagaagcc gaagaaaaa cgcaaggaa gaacagagg aagaaggaa gaggaagac gctctgcac acaaacaag agcaacgta aagttaaag gcgtcagat atttattca gatgacagc gaaagtgaa ggaggtaaa agttcacct cagccacca agacaatct gtggggtct gtcagatcc cgctcttcg tctagttca agttcgagt gataatgaa caagaagaa gaggaaaaa cccgcttat gtacctacg aaacttgac atgaataat attcggttg tcgagacat aaattagaa agatttgta catatgccg ttctttgat cgtattgtt aaaggatgt tacgtaaaa atcggtatt gggcaacat aataatatg cctgtttat cgggtggcc gaagtcaca aatgtttat gaaactgcc aagatttac aatttggga aacaccaga acaaacaag ggattgcgt gtacgacac ggaaatcag gagcgagtt ttccgccta gaatttgta tccaatcaa gaattcacc gagactgag tatcacaag tggatagag gccagcaca gctgctagt aatccttta cccacaaaa gaacacatt gaacagaaa caagccgac ataaaagaa gccttaaat tacgaattt aacgaacag gatattgaa cgaattata cgcgaaaaa gagcggttc aagccaaac ccccacaat tatgcaatg cgcaaaacc caactaatg aaagaaaga gactcagcc ctggctcgc ggggctgaa gatttggcg agagaatta acccaaagg atctctgat ttggaagaa agggcgtca gagttggac aaaatgaga acatcgacc atttcaagt atttcatac atcaacgac cggaacagg aaacgcaac gttgaagag gccgaaaag gcgattatg gccgaagtt aaagcaaac aaaggcaaa aagatcgat gacccgttc acaagacgc tcgacaaaa ccaagaatg aatttcaag gcagctcct gaagagaaa gccgaagta gtggttgag aaaaagcca gagaaggtt gcagtggaa gtggaaaac ccgaaagta gcagcgcag ccggctcaa gttactcag gacgatctc ttctcagct catgacttt gatattaaa attgactta gatgttcct ttaccagtg gctagcgta aacgttaca cccaaaaca gtaacaaag gaccttgct ccgagacga agcttgaac ttgcaagac tacaagaag aagagagga cttatatag
- >TC001573 RA
cttgaaaaa ggaacagaa agaaatgaa aaatagtaa tggtggtgg cagttagtg gttgtgttg ttggatttt gatttaatt taaatttct gaagcaaaa ttgatcatc cagtttgaa atcccaaga tgggaaaac gcaaaaatc aagctttga ttgacagtg attcgagca atgattcaa ctagcgatt tggactctg aatttttga atttggcga agaaaaaga acaggaaga agaaatcgc caaggtcta actcggatt cggaatacg agggcaaat ctgacagtg atgagggtc gaaataaca agaaaccca aacgagatg agtcctctg actccgaat ttgaaaaga aagaggagg cgaaaaata actcagagc ctgaggaag gtgaagtct cagactctt caatatctg atgaagaat ataacgacg ggtatgacg accaactta tgggcgatg aagaagaca gagcccgct tagccagtt taactgaga aagaacgag aaacggaaa ttttcaaaa gaatcgagc aacgagaat tgatgaaaa cgcgatttg agattgaga aaaagttga gacaggcga agaaagcgg aaagggcac gggagaaac ctcacaagc acaaagaca aagagaaga gcagggacc acagtaaac tccaaaccg atttcagtt caatagacc acaaggaac gtagcaaag agcggaaga aaaacattg aggaaaatc gcggcaaag ttgacaaac gtgtcaacg ccatggccg aactcaaag ccagaagag aagacaagc aaaagcggg aagaagccg aagaaaaac gcaaggaag aacagagga agaaggaag aggaagacg ctctgcaca caaacaaga gcaacgtaa agttaaagg cgtcagata tttattcag atgacagcg aaagtgaag gaggtaaaa gttcacctc agccaccaa gacaatctg tggggtctg tcagatccc gctcttcgt ctagttcaa gttcgagtg ataatgaac aagaagaag aggaaaaac ccgcttatg tacctacga aacttgaca tgaataata ttcggttgt cgagacata aattagaaa gatttgtac atatgccgt tctttgatc gtattgtta aaggatgtt acgtaaaaa tcggtattg ggcaacata ataatatgc ctgtttatc gggtggccg aagtcacaa atgtttatg aaactgcca agatttaca atttgggaa acaccagaa caaacaagg gattgcgtg tacgacacg gaaatcagg agcgagttt tccgcctag aatttgtat ccaatcaag aattcaccg agactgagt atcacaagt ggatagagg ccagcacag ctgctagta atcctttac ccacaaaag aacacattg aacagaaac aagccgaca taaaagaag ccttaaatt acgaattta acgaacagg atattgaac gaattatac gcgaaaaag agcggttca agccaaacc cccacaatt atgcaatgc gcaaaaccc aactaatga aagaaagag actcagccc tggctcgcg gggctgaag atttggcga gagaattaa cccaaagga tctctgatt tggaagaaa gggcgtcag agttggaca aaatgagaa catcgacca tttcaagta tttcataca tcaacgacc ggaacagga aacgcaacg ttgaagagg ccgaaaagg cgattatgg ccgaagtta aagcaaaca aaggcaaaa agatcgatg acccgttca caagacgct cgacaaaac caagaatga atttcaagg cagctcctg aagagaaag ccgaagtag tggttgaga aaaagccag agaaggttg cagtggaag tggaaaacc cgaaagtag cagcgcagc cggctcaag ttactcagg acgatctct tctcagctc atgactttg atattaaaa ttgacttag atgttcctt taccagtgg ctagcgtaa acgttacac ccaaaacag taacaaagg accttgctc cgagacgaa gcttgaact tgcaagact acaagaaga agagaggac ttatatagt atttattaa tttattata tttttaaca aaataaatt ttagacctt aaatgttac acttaaaca ttttttatt gttaattaa gtttcattt tatttggaa acgcgttat aattttaca gtgattttt aaggaatga attaattct gtacttttg tagcctgtt gttagatct gtttgtgat tacgaattg tttcacaaa tttgaaata atagacaaa caaggaa
